Restriction map for
pFN21AA1364
(Circular) (Six-base) MAPSORT of: pFN21AA1364.seq Check: 4258 from: 1 to: 7549
pFN21AA1364 7549 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2084 2084
Size: 7549
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4797 5359 278
Size: 53 83 186 936 3261 562 2468
Fragments arranged by size:
3261 2468 936 562 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4317 4892 1937
Size: 2380 575 4594
Fragments arranged by size:
4594 2380 575
Acc65I G'GTAC_C
Cuts at: 4729 4870 4729
Size: 141 7408
AclI AA'CG_TT
Cuts at: 1563 5676 6049 1563
Size: 4113 373 3063
Fragments arranged by size:
4113 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3367 5612 6996 7264 1632
Size: 1735 2245 1384 268 1917
Fragments arranged by size:
2245 1917 1735 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7352
AflIII A'CryG_T
Cuts at: 1203 6448 7425 1203
Size: 5245 977 1327
Fragments arranged by size:
5245 1327 977
AgeI A'CCGG_T
Cuts at: 2055 6380 2055
Size: 4325 3224
AhdI GACnn_n'nnGTC
Cuts at: 6278 6278
Size: 7549
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7549
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7549
AlwNI CAG_nnn'CTG
Cuts at: 2836 3055 4192 4276 5292 6864 2836
Size: 219 1137 84 1016 1572 3521
Fragments arranged by size:
3521 1572 1137 1016 219 84
ApaLI G'TGCA_C
Cuts at: 2254 2615 5606 6762 2254
Size: 361 2991 1156 3041
Fragments arranged by size:
3041 2991 1156 361
ApoI r'AATT_y
Cuts at: 1493 2549 2648 2711 2746 2822 3517 4199 4436
Size: 1056 99 63 35 76 695 682 237
Cuts at: 4436 4858 5084 5236 7345 1493
Size: 422 226 152 2109 1697
Fragments arranged by size:
2109 1697 1056 695 682 422 237 226
152 99 76 63 35
AseI AT'TA_AT
Cuts at: 160 1032 6103 160
Size: 872 5071 1606
Fragments arranged by size:
5071 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7549
AvaI C'yCGr_G
Cuts at: 1870 1943 2982 3240 4083 4874 1870
Size: 73 1039 258 843 791 4545
Fragments arranged by size:
4545 1039 843 791 258 73
AvrII C'CTAG_G
Cuts at: 2521 2521
Size: 7549
BamHI G'GATC_C
Cuts at: 4879 5246 4879
Size: 367 7182
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2426 3585 4547 4661 4729
Size: 325 217 627 639 1159 962 114 68
Cuts at: 4729 4781 4827 4870 6325 618
Size: 52 46 43 1455 1842
Fragments arranged by size:
1842 1455 1159 962 639 627 325 217
114 68 52 46 43
BanII G_rGCy'C
Cuts at: 1391 2109 2973 3305 4250 4638 4868 1391
Size: 718 864 332 945 388 230 4072
Fragments arranged by size:
4072 945 864 718 388 332 230
BbeI G_GCGC'C
Cuts at: 4551 4785 4831 4551
Size: 234 46 7269
Fragments arranged by size:
7269 234 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6578
BbvCI CC'TCA_GC
Cuts at: 4713 4713
Size: 7549
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 5740 5774 1903
Size: 34 2234 34 1535 34 3678
Fragments arranged by size:
3678 2234 1535 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2489 3426 5441 6651 2489
Size: 937 2015 1210 3387
Fragments arranged by size:
3387 2015 1210 937
BclI T'GATC_A
Cuts at: 1548 2658 1548
Size: 1110 6439
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2201 2741 3026
Size: 107 122 71 631 605 529 540 285
Cuts at: 3026 3217 6160 136
Size: 191 2943 1525
Fragments arranged by size:
2943 1525 631 605 540 529 285 191
122 107 71
BglII A'GATC_T
Cuts at: 3628 7544 3628
Size: 3916 3633
BlpI GC'TnA_GC
Cuts at: 3011 4966 3011
Size: 1955 5594
Bme1580I G_kGCm'C
Cuts at: 1790 2258 2611 2619 4664 5610 6766 1790
Size: 468 353 8 2045 946 1156 2573
Fragments arranged by size:
2573 2045 1156 946 468 353 8
BmgBI CAC'GTC
Cuts at: 1318 2539 1318
Size: 1221 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2336 5280 6238
Size: 416 53 83 399 24 919 2944 958
Cuts at: 6238 442
Size: 1753
Fragments arranged by size:
2944 1753 958 919 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2478 4649 4703 6209 1239
Size: 1239 2171 54 1506 2579
Fragments arranged by size:
2579 2171 1506 1239 54
Bpu10I CC'TnA_GC
Cuts at: 3965 4713 4760 3965
Size: 748 47 6754
Fragments arranged by size:
6754 748 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2075 2147 5674 6539 6837 2075
Size: 72 3527 865 298 2787
Fragments arranged by size:
3527 2787 865 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 2303 3294 4486 6212 882
Size: 617 519 285 991 1192 1726 2219
Fragments arranged by size:
2219 1726 1192 991 617 519 285
BsaAI yAC'GTr
Cuts at: 493 1204 4321 5340 493
Size: 711 3117 1019 2702
Fragments arranged by size:
3117 2702 1019 711
BsaBI GATnn'nnATC
Cuts at: 7543 7543
Size: 7549
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4548 4782 4794 4828
Size: 53 83 186 936 3015 234 12 34
Cuts at: 4828 5356 5738 6373 275
Size: 528 382 635 1451
Fragments arranged by size:
3015 1451 936 635 528 382 234 186
83 53 34 12
BsaWI w'CCGG_w
Cuts at: 2055 4359 4732 5982 6380 6654 6801 7212 2055
Size: 2304 373 1250 398 274 147 411 2392
Fragments arranged by size:
2392 2304 1250 411 398 373 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 1178
Size: 30 165 30 726 30 6568
Fragments arranged by size:
6568 726 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 3932 4060 4071 4082 4085 4110 4646
Size: 838 1908 128 11 11 3 25 536
Cuts at: 4646 4751 4802 1186
Size: 105 51 3933
Fragments arranged by size:
3933 1908 838 536 128 105 51 25
11 11 3
BseYI C'CCAG_C
Cuts at: 2035 3045 3577 4037 4185 4637 6752 7248 2035
Size: 1010 532 460 148 452 2115 496 2336
Fragments arranged by size:
2336 2115 1010 532 496 460 452 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6976
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4999 5760 6788 1540
Size: 275 186 2998 761 1028 2301
Fragments arranged by size:
2998 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2109 2258 2619 2973 4121 4755 5610 5695 6766
Size: 149 361 354 1148 634 855 85 1071
Cuts at: 6766 2109
Size: 2892
Fragments arranged by size:
2892 1148 1071 855 634 361 354 149
85
BsmI GAATG_Cn'
Cuts at: 4216 5060 5153 4216
Size: 844 93 6612
Fragments arranged by size:
6612 844 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3066 3066
Size: 7549
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2258 2611 2619 2973 3305 4121
Size: 399 319 149 353 8 354 332 816
Cuts at: 4121 4250 4638 4664 4755 4868 5610 5695 6766
Size: 129 388 26 91 113 742 85 1071
Cuts at: 6766 1391
Size: 2174
Fragments arranged by size:
2174 1071 816 742 399 388 354 353
332 319 149 129 113 91 85 26
8
BspEI T'CCGG_A
Cuts at: 7212 7212
Size: 7549
BspHI T'CATG_A
Cuts at: 3789 5438 7168 3789
Size: 1649 1730 4170
Fragments arranged by size:
4170 1730 1649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2084 4794 4904 844
Size: 1240 2710 110 3489
Fragments arranged by size:
3489 2710 1240 110
BsrBI CCG'CTC
Cuts at: 1394 1823 2145 4672 5436 1394
Size: 429 322 2527 764 3507
Fragments arranged by size:
3507 2527 764 429 322
BsrDI GCAATG_nn'
Cuts at: 65 1248 3553 3756 6044 6218 65
Size: 1183 2305 203 2288 174 1396
Fragments arranged by size:
2305 2288 1396 1183 203 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 4495 6193 6380 1700
Size: 110 245 2440 1698 187 2869
Fragments arranged by size:
2869 2440 1698 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7549
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7549
BssSI C'ACGA_G
Cuts at: 5609 6621 5609
Size: 1012 6537
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2926 3055 794
Size: 2132 129 5288
Fragments arranged by size:
5288 2132 129
BstBI TT'CG_AA
Cuts at: 6353 6353
Size: 7549
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7549
BstXI CCAn_nnnn'nTGG
Cuts at: 4582 4582
Size: 7549
BstYI r'GATC_y
Cuts at: 1972 3628 3947 4879 5246 5631 5648 7100 7186
Size: 1656 319 932 367 385 17 1452 86
Cuts at: 7186 7198 7544 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1656 1452 932 385 367 346 319
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2574 4703 2574
Size: 2129 5420
BtgI C'CryG_G
Cuts at: 513 1065 2004 2716 3813 3837 4581 513
Size: 552 939 712 1097 24 744 3481
Fragments arranged by size:
3481 1097 939 744 712 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2716 4627 487
Size: 683 56 1490 1911 3409
Fragments arranged by size:
3409 1911 1490 683 56
BtsI GCAGTG_nn'
Cuts at: 4506 4718 5065 5859 5879 4506
Size: 212 347 794 20 6176
Fragments arranged by size:
6176 794 347 212 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7549
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2836 2871 529
Size: 35 2272 35 5207
Fragments arranged by size:
5207 2272 35 35
DraI TTT'AAA
Cuts at: 4853 4853
Size: 7549
DraIII CAC_nnn'GTG
Cuts at: 2268 2268
Size: 7549
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2060 6556 809
Size: 507 744 4496 1802
Fragments arranged by size:
4496 1802 744 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2393 2727 3102
Size: 54 1425 50 275 18 563 334 375
Cuts at: 3102 3218 3443 3976 4194 4584 4996 5885 7514
Size: 116 225 533 218 390 412 889 1629
Cuts at: 7514 8
Size: 43
Fragments arranged by size:
1629 1425 889 563 533 412 390 375
334 275 225 218 116 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4996 1537
Size: 275 3184 4090
Fragments arranged by size:
4090 3184 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2781 4037 4200 4484 5479 1353
Size: 1428 1256 163 284 995 3423
Fragments arranged by size:
3423 1428 1256 995 284 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6136 6510 6656 387
Size: 1064 138 4547 374 146 1280
Fragments arranged by size:
4547 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2107 2971 2107
Size: 864 6685
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2478 3367 4649 4703 5612 6209 6996
Size: 393 846 889 1282 54 909 597 787
Cuts at: 6996 7264 1239
Size: 268 1524
Fragments arranged by size:
1524 1282 909 889 846 787 597 393
268 54
EcoNI CCTnn'n_nnAGG
Cuts at: 4706 4706
Size: 7549
EcoO109I rG'GnC_Cy
Cuts at: 4532 4532
Size: 7549
EcoRI G'AATT_C
Cuts at: 2549 2711 3517 4858 5236 2549
Size: 162 806 1341 378 4862
Fragments arranged by size:
4862 1341 806 378 162
FspI TGC'GCA
Cuts at: 6055 6055
Size: 7549
HaeII r_GCGC'y
Cuts at: 1678 4551 4785 4831 6696 1678
Size: 2873 234 46 1865 2531
Fragments arranged by size:
2873 2531 1865 234 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2351 2383 3818
Size: 32 135 32 557 32 190 32 1435
Cuts at: 3818 3850 3939 3971 3973 4005 6190 6222 6264
Size: 32 89 32 2 32 2185 32 42
Cuts at: 6264 6296 1373
Size: 32 2626
Fragments arranged by size:
2626 2185 1435 557 190 135 89 42
32 32 32 32 32 32 32 32
32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3433 4893 5139 669
Size: 988 281 1495 1460 246 3079
Fragments arranged by size:
3079 1495 1460 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2171 748
Size: 1423 6126
HpaI GTT'AAC
Cuts at: 5139 5139
Size: 7549
KasI G'GCGC_C
Cuts at: 4547 4781 4827 4547
Size: 234 46 7269
Fragments arranged by size:
7269 234 46
KpnI G_GTAC'C
Cuts at: 4733 4874 4733
Size: 141 7408
MfeI C'AATT_G
Cuts at: 5148 5148
Size: 7549
MluI A'CGCG_T
Cuts at: 7425 7425
Size: 7549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2515 2657 2684 6663 6847 1305
Size: 1210 142 27 3979 184 2007
Fragments arranged by size:
3979 2007 1210 184 142 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2395 2729 3104 3220 3978
Size: 54 1425 343 563 334 375 116 758
Cuts at: 3978 4196 4586 10
Size: 218 390 2973
Fragments arranged by size:
2973 1425 758 563 390 375 343 334
218 116 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 3122 3465 3842 5507 5866 6025
Size: 648 1375 581 343 377 1665 359 159
Cuts at: 6025 518
Size: 2042
Fragments arranged by size:
2042 1665 1375 648 581 377 359 343
159
MspA1I CmG'CkG
Cuts at: 1606 3055 4189 4965 5642 6790 7035 1606
Size: 1449 1134 776 677 1148 245 2120
Fragments arranged by size:
2120 1449 1148 1134 776 677 245
NarI GG'CG_CC
Cuts at: 4548 4782 4828 4548
Size: 234 46 7269
Fragments arranged by size:
7269 234 46
NcoI C'CATG_G
Cuts at: 513 1065 2004 3837 513
Size: 552 939 1833 4225
Fragments arranged by size:
4225 1833 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7549
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7549
NotI GC'GGCC_GC
Cuts at: 4996 4996
Size: 7549
NspI r_CATG'y
Cuts at: 1132 1229 4296 4907 6452 1132
Size: 97 3067 611 1545 2229
Fragments arranged by size:
3067 2229 1545 611 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7549
PciI A'CATG_T
Cuts at: 6448 6448
Size: 7549
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3642 4192 1108
Size: 2534 550 4465
Fragments arranged by size:
4465 2534 550
PfoI T'CCnGG_A
Cuts at: 2100 2829 3641 2100
Size: 729 812 6008
Fragments arranged by size:
6008 812 729
PmeI GTTT'AAAC
Cuts at: 4853 4853
Size: 7549
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5618 5650 7131 7163 4334
Size: 32 1252 32 1481 32 4720
Fragments arranged by size:
4720 1481 1252 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4532 4532
Size: 7549
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7549
PsiI TTA'TAA
Cuts at: 5119 5119
Size: 7549
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 7517
PstI C_TGCA'G
Cuts at: 830 4542 4725 4825 4901 830
Size: 3712 183 100 76 3478
Fragments arranged by size:
3712 3478 183 100 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7549
PvuII CAG'CTG
Cuts at: 3055 4189 3055
Size: 1134 6415
SacI G_AGCT'C
Cuts at: 2109 2973 2109
Size: 864 6685
SalI G'TCGA_C
Cuts at: 1936 4891 1936
Size: 2955 4594
SbfI CC_TGCA'GG
Cuts at: 4542 4901 4542
Size: 359 7190
ScaI AGT'ACT
Cuts at: 4382 5797 7509 4382
Size: 1415 1712 4422
Fragments arranged by size:
4422 1712 1415
SfcI C'TryA_G
Cuts at: 826 1045 3555 4538 4721 4821 4897 6032 6713
Size: 219 2510 983 183 100 76 1135 681
Cuts at: 6713 6904 826
Size: 191 1471
Fragments arranged by size:
2510 1471 1135 983 681 219 191 183
100 76
SfoI GGC'GCC
Cuts at: 4549 4783 4829 4549
Size: 234 46 7269
Fragments arranged by size:
7269 234 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7549
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7549
SmaI CCC'GGG
Cuts at: 1872 3242 4876 1872
Size: 1370 1634 4545
Fragments arranged by size:
4545 1634 1370
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 2162 5653 6554 6816 820
Size: 197 926 147 72 3491 901 262 1553
Fragments arranged by size:
3491 1553 926 901 262 197 147 72
SnaBI TAC'GTA
Cuts at: 493 5340 493
Size: 4847 2702
SpeI A'CTAG_T
Cuts at: 152 3178 152
Size: 3026 4523
SphI G_CATG'C
Cuts at: 1132 4907 1132
Size: 3775 3774
SspI AAT'ATT
Cuts at: 5 52 5473 5
Size: 47 5421 2081
Fragments arranged by size:
5421 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7549
StyI C'CwwG_G
Cuts at: 513 1065 2004 2334 2521 2554 2688 3147 3837
Size: 552 939 330 187 33 134 459 690
Cuts at: 3837 3900 4005 4988 513
Size: 63 105 983 3074
Fragments arranged by size:
3074 983 939 690 552 459 330 187
134 105 63 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2414 4120 5576 5593 5746 5931 6376
Size: 1255 834 1706 1456 17 153 185 445
Cuts at: 6376 325
Size: 1498
Fragments arranged by size:
1706 1498 1456 1255 834 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3390 4173 4380
Size: 274 80 33 51 1443 1413 783 207
Cuts at: 4380 5795 7507 96
Size: 1415 1712 138
Fragments arranged by size:
1712 1443 1415 1413 783 274 207 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3400 3432 7509 7541 3400
Size: 32 4077 32 3408
Fragments arranged by size:
4077 3408 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3404 1316
Size: 2088 5461
XbaI T'CTAG_A
Cuts at: 4885 4885
Size: 7549
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7549
XmaI C'CCGG_G
Cuts at: 1870 3240 4874 1870
Size: 1370 1634 4545
Fragments arranged by size:
4545 1634 1370
XmnI GAAnn'nnTTC
Cuts at: 2697 2711 4262 5678 2697
Size: 14 1551 1416 4568
Fragments arranged by size:
4568 1551 1416 14
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4795 5357 276
Size: 53 83 186 936 3261 562 2468
Fragments arranged by size:
3261 2468 936 562 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI