Restriction map for
pFN21AA1352
(Circular) (Six-base) MAPSORT of: pFN21AA1352.seq Check: 1111 from: 1 to: 8233
pFN21AA1352 8233 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3759 3759
Size: 8233
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6043 278
Size: 53 83 186 936 4507 2468
Fragments arranged by size:
4507 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2601 2714 5576 1937
Size: 664 113 2862 4594
Fragments arranged by size:
4594 2862 664 113
Acc65I G'GTAC_C
Cuts at: 2229 5554 2229
Size: 3325 4908
AclI AA'CG_TT
Cuts at: 1563 6360 6733 1563
Size: 4797 373 3063
Fragments arranged by size:
4797 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 4970 6296 7680 7948 1632
Size: 918 2420 1326 1384 268 1917
Fragments arranged by size:
2420 1917 1384 1326 918 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8036
AflIII A'CryG_T
Cuts at: 1203 2193 7132 8109 1203
Size: 990 4939 977 1327
Fragments arranged by size:
4939 1327 990 977
AgeI A'CCGG_T
Cuts at: 7064 7064
Size: 8233
AhdI GACnn_n'nnGTC
Cuts at: 6962 6962
Size: 8233
AleI CACnn'nnGTG
Cuts at: 1166 3164 4600 1166
Size: 1998 1436 4799
Fragments arranged by size:
4799 1998 1436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3767 1184
Size: 2583 5650
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3447 3479 4533 4565 3447
Size: 32 1054 32 7115
Fragments arranged by size:
7115 1054 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3772 4673 5976 7548 3772
Size: 901 1303 1572 4457
Fragments arranged by size:
4457 1572 1303 901
ApaLI G'TGCA_C
Cuts at: 6290 7446 6290
Size: 1156 7077
ApoI r'AATT_y
Cuts at: 1493 2522 2699 3072 3354 3682 3992 4202 5042
Size: 1029 177 373 282 328 310 210 840
Cuts at: 5042 5469 5542 5768 5920 8029 1493
Size: 427 73 226 152 2109 1697
Fragments arranged by size:
2109 1697 1029 840 427 373 328 310
282 226 210 177 152 73
AseI AT'TA_AT
Cuts at: 160 1032 3059 6787 160
Size: 872 2027 3728 1606
Fragments arranged by size:
3728 2027 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8233
AvaI C'yCGr_G
Cuts at: 1870 1943 5558 1870
Size: 73 3615 4545
Fragments arranged by size:
4545 3615 73
BamHI G'GATC_C
Cuts at: 5563 5930 5563
Size: 367 7866
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2229 3040 3289 5554 7009
Size: 325 217 627 442 811 249 2265 1455
Cuts at: 7009 618
Size: 1842
Fragments arranged by size:
2265 1842 1455 811 627 442 325 249
217
BanII G_rGCy'C
Cuts at: 1391 5552 1391
Size: 4161 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3485 4781 5160 5423 928
Size: 971 1586 1296 379 263 3738
Fragments arranged by size:
3738 1586 1296 971 379 263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6424 6458 1903
Size: 34 4487 34 3678
Fragments arranged by size:
4487 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2071 4011 6125 7335 2071
Size: 1940 2114 1210 2969
Fragments arranged by size:
2969 2114 1940 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8233
BfrBI ATG'CAT
Cuts at: 2105 2105
Size: 8233
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6844 136
Size: 107 122 71 631 605 5172 1525
Fragments arranged by size:
5172 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8228 8228
Size: 8233
BlpI GC'TnA_GC
Cuts at: 5650 5650
Size: 8233
Bme1580I G_kGCm'C
Cuts at: 1790 6294 7450 1790
Size: 4504 1156 2573
Fragments arranged by size:
4504 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8233
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4354 5964 6922
Size: 416 53 83 399 24 2937 1610 958
Cuts at: 6922 442
Size: 1753
Fragments arranged by size:
2937 1753 1610 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2790 3528 5024 6893 1239
Size: 1551 738 1496 1869 2579
Fragments arranged by size:
2579 1869 1551 1496 738
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2840 3709 4727 5117 6358 7223 7521 2840
Size: 869 1018 390 1241 865 298 3552
Fragments arranged by size:
3552 1241 1018 869 865 390 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2888 3665 6896 882
Size: 617 1389 777 3231 2219
Fragments arranged by size:
3231 2219 1389 777 617
BsaAI yAC'GTr
Cuts at: 493 1204 4871 6024 493
Size: 711 3667 1153 2702
Fragments arranged by size:
3667 2702 1153 711
BsaBI GATnn'nnATC
Cuts at: 2634 8227 2634
Size: 5593 2640
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2176 2608 6040 6422
Size: 53 83 186 936 643 432 3432 382
Cuts at: 6422 7057 275
Size: 635 1451
Fragments arranged by size:
3432 1451 936 643 635 432 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4551 6666 7064 7338 7485 7896 4551
Size: 2115 398 274 147 411 4888
Fragments arranged by size:
4888 2115 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2763 2793 4119 4149 1178
Size: 30 165 30 1360 30 1326 30 5262
Fragments arranged by size:
5262 1360 1326 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3683 4124 4568 4687 1186
Size: 2497 441 444 119 4732
Fragments arranged by size:
4732 2497 444 441 119
BseYI C'CCAG_C
Cuts at: 3871 5226 7436 7932 3871
Size: 1355 2210 496 4172
Fragments arranged by size:
4172 2210 1355 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 3078 1163
Size: 573 522 820 6318
Fragments arranged by size:
6318 820 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5683 6444 7472 1540
Size: 275 186 3682 761 1028 2301
Fragments arranged by size:
3682 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4611 6294 6379 7450 4611
Size: 1683 85 1071 5394
Fragments arranged by size:
5394 1683 1071 85
BsmI GAATG_Cn'
Cuts at: 2292 4224 4414 4654 5744 5837 2292
Size: 1932 190 240 1090 93 4688
Fragments arranged by size:
4688 1932 1090 240 190 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2948 4024 2948
Size: 1076 7157
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4611 5552 6294 6379 7450 1391
Size: 399 2821 941 742 85 1071 2174
Fragments arranged by size:
2821 2174 1071 941 742 399 85
BspEI T'CCGG_A
Cuts at: 7896 7896
Size: 8233
BspHI T'CATG_A
Cuts at: 2755 5457 6122 7852 2755
Size: 2702 665 1730 3136
Fragments arranged by size:
3136 2702 1730 665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3759 4280 4658 5588 844
Size: 2915 521 378 930 3489
Fragments arranged by size:
3489 2915 930 521 378
BsrBI CCG'CTC
Cuts at: 1394 1823 6120 1394
Size: 429 4297 3507
Fragments arranged by size:
4297 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4807 4917 6728 6902 65
Size: 1183 3559 110 1811 174 1396
Fragments arranged by size:
3559 1811 1396 1183 174 110
BsrFI r'CCGG_y
Cuts at: 1700 1810 3286 6877 7064 1700
Size: 110 1476 3591 187 2869
Fragments arranged by size:
3591 2869 1476 187 110
BsrGI T'GTAC_A
Cuts at: 96 4305 96
Size: 4209 4024
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8233
BssSI C'ACGA_G
Cuts at: 6293 7305 6293
Size: 1012 7221
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8233
BstBI TT'CG_AA
Cuts at: 7037 7037
Size: 8233
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8233
BstXI CCAn_nnnn'nTGG
Cuts at: 4270 4822 4270
Size: 552 7681
BstYI r'GATC_y
Cuts at: 1972 2459 4030 5563 5930 6315 6332 7784 7870
Size: 487 1571 1533 367 385 17 1452 86
Cuts at: 7870 7882 8228 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1571 1533 1452 487 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2715 2715
Size: 8233
Bsu36I CC'TnA_GG
Cuts at: 2787 3697 2787
Size: 910 7323
BtgI C'CryG_G
Cuts at: 513 1065 2004 4278 513
Size: 552 939 2274 4468
Fragments arranged by size:
4468 2274 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7494
Fragments arranged by size:
7494 683 56
BtsI GCAGTG_nn'
Cuts at: 3773 5749 6543 6563 3773
Size: 1976 794 20 5443
Fragments arranged by size:
5443 1976 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8233
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8198
DraI TTT'AAA
Cuts at: 2580 3451 4456 5537 2580
Size: 871 1005 1081 5276
Fragments arranged by size:
5276 1081 1005 871
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4913 7240 809
Size: 507 3597 2327 1802
Fragments arranged by size:
3597 2327 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4082 5239 5680
Size: 54 1425 50 275 18 2252 1157 441
Cuts at: 5680 6569 8198 8
Size: 889 1629 43
Fragments arranged by size:
2252 1629 1425 1157 889 441 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5680 1537
Size: 275 3868 4090
Fragments arranged by size:
4090 3868 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2361 2367 2505 3606 6163 1353
Size: 1008 6 138 1101 2557 3423
Fragments arranged by size:
3423 2557 1101 1008 138 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 6820 7194 7340 387
Size: 1064 138 435 4796 374 146 1280
Fragments arranged by size:
4796 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2550 2790 3528 4970 5024 6296 6893
Size: 393 918 240 738 1442 54 1272 597
Cuts at: 6893 7680 7948 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1442 1272 918 787 738 597 393
268 240 54
EcoNI CCTnn'n_nnAGG
Cuts at: 3833 4665 5399 3833
Size: 832 734 6667
Fragments arranged by size:
6667 832 734
EcoO109I rG'GnC_Cy
Cuts at: 4670 5015 5375 4670
Size: 345 360 7528
Fragments arranged by size:
7528 360 345
EcoRI G'AATT_C
Cuts at: 3992 5542 5920 3992
Size: 1550 378 6305
Fragments arranged by size:
6305 1550 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3198 3253 3198
Size: 55 8178
FspI TGC'GCA
Cuts at: 6739 6739
Size: 8233
HaeII r_GCGC'y
Cuts at: 1678 7380 1678
Size: 5702 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4119 4139 4151 4171 4725
Size: 32 135 32 2547 20 12 20 554
Cuts at: 4725 4757 5002 5034 5376 5408 6874 6906 6948
Size: 32 245 32 342 32 1466 32 42
Cuts at: 6948 6980 1373
Size: 32 2626
Fragments arranged by size:
2626 2547 1466 554 342 245 135 42
32 32 32 32 32 32 32 20
20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 5577 5823 669
Size: 988 281 3639 246 3079
Fragments arranged by size:
3639 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4461 748
Size: 3713 4520
HpaI GTT'AAC
Cuts at: 5823 5823
Size: 8233
KpnI G_GTAC'C
Cuts at: 2233 5558 2233
Size: 3325 4908
MfeI C'AATT_G
Cuts at: 5832 5832
Size: 8233
MluI A'CGCG_T
Cuts at: 8109 8109
Size: 8233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2089 2761 3210 7347 7531 1305
Size: 784 672 449 4137 184 2007
Fragments arranged by size:
4137 2007 784 672 449 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5241 10
Size: 54 1425 343 3409 3002
Fragments arranged by size:
3409 3002 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3164 3437 3637 4600 4602 4820 6191
Size: 648 1998 273 200 963 2 218 1371
Cuts at: 6191 6550 6709 518
Size: 359 159 2042
Fragments arranged by size:
2042 1998 1371 963 648 359 273 218
200 159 2
MspA1I CmG'CkG
Cuts at: 1606 3871 4112 5649 6326 7474 7719 1606
Size: 2265 241 1537 677 1148 245 2120
Fragments arranged by size:
2265 2120 1537 1148 677 245 241
NcoI C'CATG_G
Cuts at: 513 1065 2004 4278 513
Size: 552 939 2274 4468
Fragments arranged by size:
4468 2274 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8233
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8233
NotI GC'GGCC_GC
Cuts at: 5680 5680
Size: 8233
NsiI A_TGCA'T
Cuts at: 2107 2107
Size: 8233
NspI r_CATG'y
Cuts at: 1132 1229 2304 3719 5438 5591 7136 1132
Size: 97 1075 1415 1719 153 1545 2229
Fragments arranged by size:
2229 1719 1545 1415 1075 153 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8233
PciI A'CATG_T
Cuts at: 7132 7132
Size: 8233
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3314 5237 1108
Size: 2206 1923 4104
Fragments arranged by size:
4104 2206 1923
PmeI GTTT'AAAC
Cuts at: 5537 5537
Size: 8233
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6302 6334 7815 7847 6302
Size: 32 1481 32 6688
Fragments arranged by size:
6688 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4670 4670
Size: 8233
PshAI GACnn'nnGTC
Cuts at: 1613 2607 5160 1613
Size: 994 2553 4686
Fragments arranged by size:
4686 2553 994
PsiI TTA'TAA
Cuts at: 4065 4421 5803 4065
Size: 356 1382 6495
Fragments arranged by size:
6495 1382 356
PstI C_TGCA'G
Cuts at: 830 4797 5585 830
Size: 3967 788 3478
Fragments arranged by size:
3967 3478 788
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8233
PvuII CAG'CTG
Cuts at: 4112 4112
Size: 8233
SalI G'TCGA_C
Cuts at: 1936 5575 1936
Size: 3639 4594
SbfI CC_TGCA'GG
Cuts at: 5585 5585
Size: 8233
ScaI AGT'ACT
Cuts at: 6481 8193 6481
Size: 1712 6521
SexAI A'CCwGG_T
Cuts at: 5197 5197
Size: 8233
SfcI C'TryA_G
Cuts at: 826 1045 2223 4107 4251 4793 5581 6716 7397
Size: 219 1178 1884 144 542 788 1135 681
Cuts at: 7397 7588 826
Size: 191 1471
Fragments arranged by size:
1884 1471 1178 1135 788 681 542 219
191 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8233
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8233
SmaI CCC'GGG
Cuts at: 1872 5560 1872
Size: 3688 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2819 3724 4742 5096 6337 7238
Size: 197 926 876 905 1018 354 1241 901
Cuts at: 7238 7500 820
Size: 262 1553
Fragments arranged by size:
1553 1241 1018 926 905 901 876 354
262 197
SnaBI TAC'GTA
Cuts at: 493 4871 6024 493
Size: 4378 1153 2702
Fragments arranged by size:
4378 2702 1153
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8233
SphI G_CATG'C
Cuts at: 1132 5438 5591 1132
Size: 4306 153 3774
Fragments arranged by size:
4306 3774 153
SspI AAT'ATT
Cuts at: 5 52 2863 6157 5
Size: 47 2811 3294 2081
Fragments arranged by size:
3294 2811 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8233
StyI C'CwwG_G
Cuts at: 513 1065 2004 2494 3755 3897 4278 4841 5672
Size: 552 939 490 1261 142 381 563 831
Cuts at: 5672 513
Size: 3074
Fragments arranged by size:
3074 1261 939 831 563 552 490 381
142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4312 6260 6277 6430 6615 7060 325
Size: 1255 2732 1948 17 153 185 445 1498
Fragments arranged by size:
2732 1948 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2279 2940 4305
Size: 274 80 33 51 1443 302 661 1365
Cuts at: 4305 5406 6479 8191 96
Size: 1101 1073 1712 138
Fragments arranged by size:
1712 1443 1365 1101 1073 661 302 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3752 3784 8193 8225 3752
Size: 32 4409 32 3760
Fragments arranged by size:
4409 3760 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3269 1316
Size: 1953 6280
XbaI T'CTAG_A
Cuts at: 5569 5569
Size: 8233
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3802 5234 3802
Size: 1432 6801
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8233
XmaI C'CCGG_G
Cuts at: 1870 5558 1870
Size: 3688 4545
XmnI GAAnn'nnTTC
Cuts at: 3322 6362 3322
Size: 3040 5193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6041 276
Size: 53 83 186 936 4507 2468
Fragments arranged by size:
4507 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI