Restriction map for
pFN21AA1332
(Circular) (Six-base) MAPSORT of: pFN21AA1332.seq Check: 7162 from: 1 to: 6856
pFN21AA1332 6856 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4666 278
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4199 1937
Size: 2262 4594
Acc65I G'GTAC_C
Cuts at: 4177 4177
Size: 6856
AclI AA'CG_TT
Cuts at: 1563 4983 5356 1563
Size: 3420 373 3063
Fragments arranged by size:
3420 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2487 3049 3249 3438 4919 6303 6571 1632
Size: 855 562 200 189 1481 1384 268 1917
Fragments arranged by size:
1917 1481 1384 855 562 268 200 189
AfeI AGC'GCT
Cuts at: 2360 2360
Size: 6856
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6659
AflIII A'CryG_T
Cuts at: 1203 5755 6732 1203
Size: 4552 977 1327
Fragments arranged by size:
4552 1327 977
AgeI A'CCGG_T
Cuts at: 5687 5687
Size: 6856
AhdI GACnn_n'nnGTC
Cuts at: 5585 5585
Size: 6856
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3950 1184
Size: 2766 4090
AlwNI CAG_nnn'CTG
Cuts at: 3821 4599 6171 3821
Size: 778 1572 4506
Fragments arranged by size:
4506 1572 778
ApaI G_GGCC'C
Cuts at: 3013 3013
Size: 6856
ApaLI G'TGCA_C
Cuts at: 4913 6069 4913
Size: 1156 5700
ApoI r'AATT_y
Cuts at: 1493 4165 4391 4543 6652 1493
Size: 2672 226 152 2109 1697
Fragments arranged by size:
2672 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5410 160
Size: 872 4378 1606
Fragments arranged by size:
4378 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
AvaI C'yCGr_G
Cuts at: 1870 1943 2850 3162 3273 4181 1870
Size: 73 907 312 111 908 4545
Fragments arranged by size:
4545 908 907 312 111 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2222 2255 2222
Size: 33 6823
BamHI G'GATC_C
Cuts at: 2487 2759 3497 4186 4553 2487
Size: 272 738 689 367 4790
Fragments arranged by size:
4790 738 689 367 272
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3024 3665 4177 5632 618
Size: 325 217 627 1237 641 512 1455 1842
Fragments arranged by size:
1842 1455 1237 641 627 512 325 217
BanII G_rGCy'C
Cuts at: 1391 2100 3013 3091 3250 4175 1391
Size: 709 913 78 159 925 4072
Fragments arranged by size:
4072 925 913 709 159 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3055 3744 928
Size: 971 1156 689 4040
Fragments arranged by size:
4040 1156 971 689
BbvCI CC'TCA_GC
Cuts at: 2118 2118
Size: 6856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5047 5081 1903
Size: 34 3110 34 3678
Fragments arranged by size:
3678 3110 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3416 4748 5958 3416
Size: 1332 1210 4314
Fragments arranged by size:
4314 1332 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6856
BfrBI ATG'CAT
Cuts at: 2956 2956
Size: 6856
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3867 5467 136
Size: 107 122 71 631 605 2195 1600 1525
Fragments arranged by size:
2195 1600 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3484 3505 6851 3484
Size: 21 3346 3489
Fragments arranged by size:
3489 3346 21
BlpI GC'TnA_GC
Cuts at: 4273 4273
Size: 6856
Bme1580I G_kGCm'C
Cuts at: 1790 3013 3643 3670 3804 4917 6073 1790
Size: 1223 630 27 134 1113 1156 2573
Fragments arranged by size:
2573 1223 1156 1113 630 134 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6856
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 2832 3096
Size: 416 53 83 399 24 682 733 264
Cuts at: 3096 4587 5545 442
Size: 1491 958 1753
Fragments arranged by size:
1753 1491 958 733 682 416 399 264
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6856
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3190 3354 3609 3110
Size: 80 164 255 6357
Fragments arranged by size:
6357 255 164 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2479 2550 3126 3168 3324 3741 5516 1239
Size: 1240 71 576 42 156 417 1775 2579
Fragments arranged by size:
2579 1775 1240 576 417 156 71 42
Bpu10I CC'TnA_GC
Cuts at: 2118 2118
Size: 6856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2819 2969 4981 5846 6144 2819
Size: 150 2012 865 298 3531
Fragments arranged by size:
3531 2012 865 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3232 5519 882
Size: 617 1733 2287 2219
Fragments arranged by size:
2287 2219 1733 617
BsaAI yAC'GTr
Cuts at: 493 1204 3631 3946 4647 493
Size: 711 2427 315 701 2702
Fragments arranged by size:
2702 2427 711 701 315
BsaBI GATnn'nnATC
Cuts at: 2391 3496 6850 2391
Size: 1105 3354 2397
Fragments arranged by size:
3354 2397 1105
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4663 5045 5680 275
Size: 53 83 186 936 3130 382 635 1451
Fragments arranged by size:
3130 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5289 5687 5961 6108 6519 5289
Size: 398 274 147 411 5626
Fragments arranged by size:
5626 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3763 3783 3793 3813 1178
Size: 30 165 30 2360 20 10 20 4221
Fragments arranged by size:
4221 2360 165 30 30 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2005 2108 2135 2701 3129 3315 3732 1186
Size: 819 103 27 566 428 186 417 4310
Fragments arranged by size:
4310 819 566 428 417 186 103 27
BseYI C'CCAG_C
Cuts at: 3225 3292 6059 6555 3225
Size: 67 2767 496 3526
Fragments arranged by size:
3526 2767 496 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6283
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 4306 5067 6095 1540
Size: 275 186 1222 1083 761 1028 2301
Fragments arranged by size:
2301 1222 1083 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4917 5002 6073 4917
Size: 85 1071 5700
Fragments arranged by size:
5700 1071 85
BsmI GAATG_Cn'
Cuts at: 2956 4367 4460 2956
Size: 1411 93 5352
Fragments arranged by size:
5352 1411 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3297 3661 3297
Size: 364 6492
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 3013 3091 3250 3643 3670 3804
Size: 399 310 913 78 159 393 27 134
Cuts at: 3804 4175 4917 5002 6073 1391
Size: 371 742 85 1071 2174
Fragments arranged by size:
2174 1071 913 742 399 393 371 310
159 134 85 78 27
BspEI T'CCGG_A
Cuts at: 6519 6519
Size: 6856
BspHI T'CATG_A
Cuts at: 2283 4745 6475 2283
Size: 2462 1730 2664
Fragments arranged by size:
2664 2462 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4211 844
Size: 3367 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3174 4743 1394
Size: 429 1351 1569 3507
Fragments arranged by size:
3507 1569 1351 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2443 2782 3852 5351 5525 65
Size: 1183 1195 339 1070 1499 174 1396
Fragments arranged by size:
1499 1396 1195 1183 1070 339 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5500 5687 1700
Size: 110 3690 187 2869
Fragments arranged by size:
3690 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2547 3382 96
Size: 2451 835 3570
Fragments arranged by size:
3570 2451 835
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6856
BssSI C'ACGA_G
Cuts at: 4916 5928 4916
Size: 1012 5844
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6856
BstBI TT'CG_AA
Cuts at: 5660 5660
Size: 6856
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6856
BstXI CCAn_nnnn'nTGG
Cuts at: 3021 3833 3021
Size: 812 6044
BstYI r'GATC_y
Cuts at: 1972 2487 2759 3484 3497 3505 3517 4186 4553
Size: 515 272 725 13 8 12 669 367
Cuts at: 4553 4938 4955 6407 6493 6505 6851 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 725 669 515 385 367 346
272 86 17 13 12 12 8
BtgI C'CryG_G
Cuts at: 513 1065 2004 2683 2697 3546 4044 513
Size: 552 939 679 14 849 498 3325
Fragments arranged by size:
3325 939 849 679 552 498 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3713 487
Size: 683 56 2487 3630
Fragments arranged by size:
3630 2487 683 56
BtsI GCAGTG_nn'
Cuts at: 2810 3793 4372 5166 5186 2810
Size: 983 579 794 20 4480
Fragments arranged by size:
4480 983 794 579 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6821
DraI TTT'AAA
Cuts at: 4160 4160
Size: 6856
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5863 809
Size: 507 4547 1802
Fragments arranged by size:
4547 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2585 2704
Size: 54 1425 50 275 18 177 578 119
Cuts at: 2704 2857 3220 3633 4303 5192 6821 8
Size: 153 363 413 670 889 1629 43
Fragments arranged by size:
1629 1425 889 670 578 413 363 275
177 153 119 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 4303 1537
Size: 275 1408 1083 4090
Fragments arranged by size:
4090 1408 1083 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 2994 3370 4786 1353
Size: 801 840 376 1416 3423
Fragments arranged by size:
3423 1416 840 801 376
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5443 5817 5963 387
Size: 1064 138 3854 374 146 1280
Fragments arranged by size:
3854 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2479 2487 2550 3049 3126 3168 3249
Size: 393 847 8 63 499 77 42 81
Cuts at: 3249 3324 3438 3741 4919 5516 6303 6571 1239
Size: 75 114 303 1178 597 787 268 1524
Fragments arranged by size:
1524 1178 847 787 597 499 393 303
268 114 81 77 75 63 42 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3813 3825 3813
Size: 12 6844
EcoO109I rG'GnC_Cy
Cuts at: 2508 2788 3009 3010 3713 3818 2508
Size: 280 221 1 703 105 5546
Fragments arranged by size:
5546 703 280 221 105 1
EcoRI G'AATT_C
Cuts at: 4165 4543 4165
Size: 378 6478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3302 3302
Size: 6856
FspI TGC'GCA
Cuts at: 5362 5362
Size: 6856
HaeII r_GCGC'y
Cuts at: 1678 2362 6003 1678
Size: 684 3641 2531
Fragments arranged by size:
3641 2531 684
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2068 2100 5497 5529 5571
Size: 32 135 32 496 32 3397 32 42
Cuts at: 5571 5603 1373
Size: 32 2626
Fragments arranged by size:
3397 2626 496 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3217 4200 4446 669
Size: 988 281 1279 983 246 3079
Fragments arranged by size:
3079 1279 988 983 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6856
HpaI GTT'AAC
Cuts at: 3217 4446 3217
Size: 1229 5627
KpnI G_GTAC'C
Cuts at: 4181 4181
Size: 6856
MfeI C'AATT_G
Cuts at: 4455 4455
Size: 6856
MluI A'CGCG_T
Cuts at: 6732 6732
Size: 6856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 2515 3656 3997 5970 6154 1305
Size: 822 388 1141 341 1973 184 2007
Fragments arranged by size:
2007 1973 1141 822 388 341 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2706 2859 3635 10
Size: 54 1425 343 177 697 153 776 3231
Fragments arranged by size:
3231 1425 776 697 343 177 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 3938 4814 5173 5332 518
Size: 648 879 1893 876 359 159 2042
Fragments arranged by size:
2042 1893 879 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2433 3225 3353 4272 4949 6097 6342 1606
Size: 827 792 128 919 677 1148 245 2120
Fragments arranged by size:
2120 1148 919 827 792 677 245 128
NcoI C'CATG_G
Cuts at: 513 1065 2004 2683 2697 3546 513
Size: 552 939 679 14 849 3823
Fragments arranged by size:
3823 939 849 679 552 14
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6856
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6856
NotI GC'GGCC_GC
Cuts at: 4303 4303
Size: 6856
NsiI A_TGCA'T
Cuts at: 2958 2958
Size: 6856
NspI r_CATG'y
Cuts at: 1132 1229 2379 2956 4214 5759 1132
Size: 97 1150 577 1258 1545 2229
Fragments arranged by size:
2229 1545 1258 1150 577 97
PasI CC'CwG_GG
Cuts at: 1348 3243 3815 1348
Size: 1895 572 4389
Fragments arranged by size:
4389 1895 572
PciI A'CATG_T
Cuts at: 5755 5755
Size: 6856
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6856
PfoI T'CCnGG_A
Cuts at: 2295 2338 3340 2295
Size: 43 1002 5811
Fragments arranged by size:
5811 1002 43
PmeI GTTT'AAAC
Cuts at: 4160 4160
Size: 6856
PmlI CAC'GTG
Cuts at: 3631 3631
Size: 6856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4925 4957 6438 6470 4925
Size: 32 1481 32 5311
Fragments arranged by size:
5311 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2508 2788 2508
Size: 280 6576
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6856
PsiI TTA'TAA
Cuts at: 4426 4426
Size: 6856
PspOMI G'GGCC_C
Cuts at: 3009 3009
Size: 6856
PspXI vC'TCGA_Gb
Cuts at: 2850 2850
Size: 6856
PstI C_TGCA'G
Cuts at: 830 3730 4208 830
Size: 2900 478 3478
Fragments arranged by size:
3478 2900 478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6856
PvuII CAG'CTG
Cuts at: 2433 3353 2433
Size: 920 5936
SalI G'TCGA_C
Cuts at: 1936 4198 1936
Size: 2262 4594
SapI GCTCTTCn'nnn_
Cuts at: 2994 3370 2994
Size: 376 6480
SbfI CC_TGCA'GG
Cuts at: 4208 4208
Size: 6856
ScaI AGT'ACT
Cuts at: 3403 4112 5104 6816 3403
Size: 709 992 1712 3443
Fragments arranged by size:
3443 1712 992 709
SfcI C'TryA_G
Cuts at: 826 1045 3726 4204 5339 6020 6211 826
Size: 219 2681 478 1135 681 191 1471
Fragments arranged by size:
2681 1471 1135 681 478 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6856
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6856
SmaI CCC'GGG
Cuts at: 1872 3275 4183 1872
Size: 1403 908 4545
Fragments arranged by size:
4545 1403 908
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2798 2850 2984 4960 5861 6123
Size: 197 926 855 52 134 1976 901 262
Cuts at: 6123 820
Size: 1553
Fragments arranged by size:
1976 1553 926 901 855 262 197 134
52
SnaBI TAC'GTA
Cuts at: 493 4647 493
Size: 4154 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6856
SphI G_CATG'C
Cuts at: 1132 2379 4214 1132
Size: 1247 1835 3774
Fragments arranged by size:
3774 1835 1247
SspI AAT'ATT
Cuts at: 5 52 4780 5
Size: 47 4728 2081
Fragments arranged by size:
4728 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6856
StyI C'CwwG_G
Cuts at: 513 1065 2004 2683 2697 2966 3546 3826 3966
Size: 552 939 679 14 269 580 280 140
Cuts at: 3966 4295 513
Size: 329 3074
Fragments arranged by size:
3074 939 679 580 552 329 280 269
140 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2118 3347 4883 4900 5053 5238 5683
Size: 1255 538 1229 1536 17 153 185 445
Cuts at: 5683 325
Size: 1498
Fragments arranged by size:
1536 1498 1255 1229 538 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 3382 3401
Size: 274 80 33 51 1443 570 835 19
Cuts at: 3401 4110 4134 5102 6814 96
Size: 709 24 968 1712 138
Fragments arranged by size:
1712 1443 968 835 709 570 274 138
80 51 33 24 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3039 3071 3783 3815 6816 6848 3039
Size: 32 712 32 3001 32 3047
Fragments arranged by size:
3047 3001 712 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6856
XbaI T'CTAG_A
Cuts at: 4192 4192
Size: 6856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3077 3553 3077
Size: 476 6380
XhoI C'TCGA_G
Cuts at: 1943 2850 1943
Size: 907 5949
XmaI C'CCGG_G
Cuts at: 1870 3273 4181 1870
Size: 1403 908 4545
Fragments arranged by size:
4545 1403 908
XmnI GAAnn'nnTTC
Cuts at: 4985 4985
Size: 6856
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4664 276
Size: 53 83 186 936 3130 2468
Fragments arranged by size:
3130 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BsiWI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI