Restriction map for
pFN21AA1319
(Circular) (Six-base) MAPSORT of: pFN21AA1319.seq Check: 102 from: 1 to: 8314
pFN21AA1319 8314 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6124 278
Size: 53 83 186 936 4588 2468
Fragments arranged by size:
4588 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2046 5222 5553 5657 1937
Size: 109 3176 331 104 4594
Fragments arranged by size:
4594 3176 331 109 104
Acc65I G'GTAC_C
Cuts at: 5635 5635
Size: 8314
AclI AA'CG_TT
Cuts at: 1563 6441 6814 1563
Size: 4878 373 3063
Fragments arranged by size:
4878 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2517 2810 3422 3524 3681 4262 4721 4916
Size: 885 293 612 102 157 581 459 195
Cuts at: 4916 5114 5214 6377 7761 8029 1632
Size: 198 100 1163 1384 268 1917
Fragments arranged by size:
1917 1384 1163 885 612 581 459 293
268 198 195 157 102 100
AflII C'TTAA_G
Cuts at: 820 1017 3926 820
Size: 197 2909 5208
Fragments arranged by size:
5208 2909 197
AflIII A'CryG_T
Cuts at: 1203 3364 3553 7213 8190 1203
Size: 2161 189 3660 977 1327
Fragments arranged by size:
3660 2161 1327 977 189
AgeI A'CCGG_T
Cuts at: 7145 7145
Size: 8314
AhdI GACnn_n'nnGTC
Cuts at: 4254 7043 4254
Size: 2789 5525
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2486 3236 3617 1184
Size: 1302 750 381 5881
Fragments arranged by size:
5881 1302 750 381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4038 4070 5247 5279 4038
Size: 32 1177 32 7073
Fragments arranged by size:
7073 1177 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2435 2773 3229 4081 4240 4312 4333 4459 4756
Size: 338 456 852 159 72 21 126 297
Cuts at: 4756 4768 4831 4840 5050 5498 5610 6057 7629
Size: 12 63 9 210 448 112 447 1572
Cuts at: 7629 2435
Size: 3120
Fragments arranged by size:
3120 1572 852 456 448 447 338 297
210 159 126 112 72 63 21 12
9
ApaI G_GGCC'C
Cuts at: 2042 3514 4471 4888 2042
Size: 1472 957 417 5468
Fragments arranged by size:
5468 1472 957 417
ApaLI G'TGCA_C
Cuts at: 2853 6371 7527 2853
Size: 3518 1156 3640
Fragments arranged by size:
3640 3518 1156
ApoI r'AATT_y
Cuts at: 1493 4205 5540 5623 5849 6001 8110 1493
Size: 2712 1335 83 226 152 2109 1697
Fragments arranged by size:
2712 2109 1697 1335 226 152 83
AseI AT'TA_AT
Cuts at: 160 1032 6868 160
Size: 872 5836 1606
Fragments arranged by size:
5836 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8314
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2583 3420 3855 4251 4904 5639
Size: 73 91 549 837 435 396 653 735
Cuts at: 5639 1870
Size: 4545
Fragments arranged by size:
4545 837 735 653 549 435 396 91
73
BamHI G'GATC_C
Cuts at: 2446 5644 6011 2446
Size: 3198 367 4749
Fragments arranged by size:
4749 3198 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 5635 7090 618
Size: 325 217 627 227 3621 1455 1842
Fragments arranged by size:
3621 1842 1455 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2034 2042 2284 2291 3276 3514 4471 4888
Size: 643 8 242 7 985 238 957 417
Cuts at: 4888 5022 5531 5633 1391
Size: 134 509 102 4072
Fragments arranged by size:
4072 985 957 643 509 417 242 238
134 102 8 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2523 4718 5102 5213 5280 928
Size: 971 624 2195 384 111 67 3962
Fragments arranged by size:
3962 2195 971 624 384 111 67
BbvCI CC'TCA_GC
Cuts at: 3473 5009 3473
Size: 1536 6778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6505 6539 1903
Size: 34 4568 34 3678
Fragments arranged by size:
4568 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6206 7416 6206
Size: 1210 7104
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2426 4391 4703
Size: 107 122 71 631 605 754 1965 312
Cuts at: 4703 4772 6925 136
Size: 69 2153 1525
Fragments arranged by size:
2153 1965 1525 754 631 605 312 122
107 71 69
BglII A'GATC_T
Cuts at: 8309 8309
Size: 8314
BlpI GC'TnA_GC
Cuts at: 2028 4419 4518 4785 5312 5491 5525 5731 2028
Size: 2391 99 267 527 179 34 206 4611
Fragments arranged by size:
4611 2391 527 267 206 179 99 34
Bme1580I G_kGCm'C
Cuts at: 1790 2042 2105 2744 2857 3514 4396 4471 4888
Size: 252 63 639 113 657 882 75 417
Cuts at: 4888 6375 7531 1790
Size: 1487 1156 2573
Fragments arranged by size:
2573 1487 1156 882 657 639 417 252
113 75 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2435 2827 6045
Size: 416 53 83 399 24 1018 392 3218
Cuts at: 6045 7003 442
Size: 958 1753
Fragments arranged by size:
3218 1753 1018 958 416 399 392 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2483 1056
Size: 1427 6887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2998 3547 4732 5485 2998
Size: 549 1185 753 5827
Fragments arranged by size:
5827 1185 753 549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2495 2874 3047 3200 3212 3254 3260 3347
Size: 1256 379 173 153 12 42 6 87
Cuts at: 3347 3653 3707 3888 4040 4058 4334 4355 4418
Size: 306 54 181 152 18 276 21 63
Cuts at: 4418 4493 4544 4604 4652 4740 4862 5072 5258
Size: 75 51 60 48 88 122 210 186
Cuts at: 5258 5423 5480 6974 1239
Size: 165 57 1494 2579
Fragments arranged by size:
2579 1494 1256 379 306 276 210 186
181 173 165 153 152 122 88 87
75 63 60 57 54 51 48 42
21 18 12 6
Bpu10I CC'TnA_GC
Cuts at: 2133 3378 3387 3473 5009 2133
Size: 1245 9 86 1536 5438
Fragments arranged by size:
5438 1536 1245 86 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3878 5174 6439 7304 7602 3878
Size: 1296 1265 865 298 4590
Fragments arranged by size:
4590 1296 1265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2898 3323 3410 4178 4907 6977 882
Size: 617 1399 425 87 768 729 2070 2219
Fragments arranged by size:
2219 2070 1399 768 729 617 425 87
BsaAI yAC'GTr
Cuts at: 493 1204 6105 493
Size: 711 4901 2702
Fragments arranged by size:
4901 2702 711
BsaBI GATnn'nnATC
Cuts at: 8308 8308
Size: 8314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2125 6121 6503 7138
Size: 53 83 186 936 592 3996 382 635
Cuts at: 7138 275
Size: 1451
Fragments arranged by size:
3996 1451 936 635 592 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3909 6747 7145 7419 7566 7977 3909
Size: 2838 398 274 147 411 4246
Fragments arranged by size:
4246 2838 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2678 2708 3476 3506 4038
Size: 30 165 30 1275 30 768 30 532
Cuts at: 4038 4068 5051 5081 1178
Size: 30 983 30 4411
Fragments arranged by size:
4411 1275 983 768 532 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2384 2852 2863 3019 3125 3209 3253 3479
Size: 1198 468 11 156 106 84 44 226
Cuts at: 3479 3536 3704 3716 3809 3821 3935 4352 4379
Size: 57 168 12 93 12 114 417 27
Cuts at: 4379 4427 4490 4541 4958 5426 1186
Size: 48 63 51 417 468 4074
Fragments arranged by size:
4074 1198 468 468 417 417 226 168
156 114 106 93 84 63 57 51
48 44 27 12 12 11
BseYI C'CCAG_C
Cuts at: 2128 2186 3255 3965 4144 4626 4674 4824 5397
Size: 58 1069 710 179 482 48 150 573
Cuts at: 5397 7517 8013 2128
Size: 2120 496 2429
Fragments arranged by size:
2429 2120 1069 710 573 496 482 179
150 58 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2112 2195 3232 1163
Size: 573 376 83 1037 6245
Fragments arranged by size:
6245 1037 573 376 83
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5764 6525 7553 1540
Size: 275 186 3763 761 1028 2301
Fragments arranged by size:
3763 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 2284 2291 2857 3276 3894 4503 5022 5531
Size: 76 7 566 419 618 609 519 509
Cuts at: 5531 6375 6460 7531 2208
Size: 844 85 1071 2991
Fragments arranged by size:
2991 1071 844 618 609 566 519 509
419 85 76 7
BsmI GAATG_Cn'
Cuts at: 4354 5825 5918 4354
Size: 1471 93 6750
Fragments arranged by size:
6750 1471 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4163 4163
Size: 8314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2042 2105 2208 2284 2291 2744
Size: 399 244 8 63 103 76 7 453
Cuts at: 2744 2857 3276 3514 3894 4396 4471 4503 4888
Size: 113 419 238 380 502 75 32 385
Cuts at: 4888 5022 5531 5633 6375 6460 7531 1391
Size: 134 509 102 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 509 502 453 419 399
385 380 244 238 134 113 103 102
85 76 75 63 32 8 7
BspEI T'CCGG_A
Cuts at: 3909 7977 3909
Size: 4068 4246
BspHI T'CATG_A
Cuts at: 6203 7933 6203
Size: 1730 6584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5669 844
Size: 4825 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3184 3487 4789 5182 5242 5281 5490
Size: 429 1361 303 1302 393 60 39 209
Cuts at: 5490 6201 1394
Size: 711 3507
Fragments arranged by size:
3507 1361 1302 711 429 393 303 209
60 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 5568 6809 6983 65
Size: 1183 4320 1241 174 1396
Fragments arranged by size:
4320 1396 1241 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4212 6958 7145 1700
Size: 110 2402 2746 187 2869
Fragments arranged by size:
2869 2746 2402 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8314
BssSI C'ACGA_G
Cuts at: 6374 7386 6374
Size: 1012 7302
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3229 4609 4768 794
Size: 2435 1380 159 4340
Fragments arranged by size:
4340 2435 1380 159
BstBI TT'CG_AA
Cuts at: 2747 7118 2747
Size: 4371 3943
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8314
BstYI r'GATC_y
Cuts at: 1972 2446 3745 4746 5644 6011 6396 6413 7865
Size: 474 1299 1001 898 367 385 17 1452
Cuts at: 7865 7951 7963 8309 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1299 1001 898 474 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2275 2969 4010 5318 5384 2275
Size: 694 1041 1308 66 5205
Fragments arranged by size:
5205 1308 1041 694 66
BtgI C'CryG_G
Cuts at: 513 1065 2004 2050 2663 2843 3075 4956 513
Size: 552 939 46 613 180 232 1881 3871
Fragments arranged by size:
3871 1881 939 613 552 232 180 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7575
Fragments arranged by size:
7575 683 56
BtsI GCAGTG_nn'
Cuts at: 2517 2938 3633 4170 5576 5830 6624 6644 2517
Size: 421 695 537 1406 254 794 20 4187
Fragments arranged by size:
4187 1406 794 695 537 421 254 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8279
DraI TTT'AAA
Cuts at: 5618 5618
Size: 8314
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2054 7321 809
Size: 507 738 5267 1802
Fragments arranged by size:
5267 1802 738 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2596 4122 4987
Size: 54 1425 50 275 18 766 1526 865
Cuts at: 4987 5106 5761 6650 8279 8
Size: 119 655 889 1629 43
Fragments arranged by size:
1629 1526 1425 889 865 766 655 275
119 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5761 1537
Size: 275 3949 4090
Fragments arranged by size:
4090 3949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3129 3201 3429 3798 4092 4356 5190 5352
Size: 1776 72 228 369 294 264 834 162
Cuts at: 5352 6244 1353
Size: 892 3423
Fragments arranged by size:
3423 1776 892 834 369 294 264 228
162 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4199 6901 7275 7421 387
Size: 1064 138 2610 2702 374 146 1280
Fragments arranged by size:
2702 2610 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2282 2289 3274 5020 5529 2282
Size: 7 985 1746 509 5067
Fragments arranged by size:
5067 1746 985 509 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2495 2517 2810 2874 3047 3200 3212
Size: 393 863 22 293 64 173 153 12
Cuts at: 3212 3254 3260 3347 3422 3524 3653 3681 3707
Size: 42 6 87 75 102 129 28 26
Cuts at: 3707 3888 4040 4058 4262 4334 4355 4418 4493
Size: 181 152 18 204 72 21 63 75
Cuts at: 4493 4544 4604 4652 4721 4740 4862 4916 5072
Size: 51 60 48 69 19 122 54 156
Cuts at: 5072 5114 5214 5258 5423 5480 6377 6974 7761
Size: 42 100 44 165 57 897 597 787
Cuts at: 7761 8029 1239
Size: 268 1524
Fragments arranged by size:
1524 897 863 787 597 393 293 268
204 181 173 165 156 153 152 129
122 102 100 87 75 75 72 69
64 63 60 57 54 51 48 44
42 42 28 26 22 21 19 18
12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2972 3001 3518 4412 4442 4475 2972
Size: 29 517 894 30 33 6811
Fragments arranged by size:
6811 894 517 33 30 29
EcoO109I rG'GnC_Cy
Cuts at: 2038 2039 2271 2379 2432 2821 2860 3374 3406
Size: 1 232 108 53 389 39 514 32
Cuts at: 3406 3510 3511 4342 4468 4765 4884 5047 5089
Size: 104 1 831 126 297 119 163 42
Cuts at: 5089 2038
Size: 5263
Fragments arranged by size:
5263 831 514 389 297 232 163 126
119 108 104 53 42 39 32 1
1
EcoRI G'AATT_C
Cuts at: 4205 5540 5623 6001 4205
Size: 1335 83 378 6518
Fragments arranged by size:
6518 1335 378 83
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3310 3827 3310
Size: 517 7797
FspI TGC'GCA
Cuts at: 6820 6820
Size: 8314
HaeII r_GCGC'y
Cuts at: 1678 2692 3292 4681 7461 1678
Size: 1014 600 1389 2780 2531
Fragments arranged by size:
2780 2531 1389 1014 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2089 2121 2678 2710 3025
Size: 32 135 32 517 32 557 32 315
Cuts at: 3025 3057 4240 4272 5051 5083 5440 5472 5549
Size: 32 1183 32 779 32 357 32 77
Cuts at: 5549 5581 6955 6987 7029 7061 1373
Size: 32 1374 32 42 32 2626
Fragments arranged by size:
2626 1374 1183 779 557 517 357 315
135 77 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2567 3495 5658 5904 669
Size: 988 281 629 928 2163 246 3079
Fragments arranged by size:
3079 2163 988 928 629 281 246
HindIII A'AGCT_T
Cuts at: 748 5481 748
Size: 4733 3581
HpaI GTT'AAC
Cuts at: 5904 5904
Size: 8314
KpnI G_GTAC'C
Cuts at: 5639 5639
Size: 8314
MfeI C'AATT_G
Cuts at: 5913 5913
Size: 8314
MluI A'CGCG_T
Cuts at: 8190 8190
Size: 8314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2570 3969 4557 7428 7612 1305
Size: 1265 1399 588 2871 184 2007
Fragments arranged by size:
2871 2007 1399 1265 588 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5108 10
Size: 54 1425 343 3276 3216
Fragments arranged by size:
3276 3216 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5292 6272 6631 6790 518
Size: 648 4126 980 359 159 2042
Fragments arranged by size:
4126 2042 980 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2845 3190 3472 3778 4000 4153 4240 4309
Size: 1239 345 282 306 222 153 87 69
Cuts at: 4309 4333 4423 4492 4630 4894 5008 5495 5730
Size: 24 90 69 138 264 114 487 235
Cuts at: 5730 6407 7555 7800 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1239 1148 677 487 345 306 282
264 245 235 222 153 138 114 90
87 69 69 24
NaeI GCC'GGC
Cuts at: 4214 4214
Size: 8314
NcoI C'CATG_G
Cuts at: 513 1065 2004 2050 513
Size: 552 939 46 6777
Fragments arranged by size:
6777 939 552 46
NdeI CA'TA_TG
Cuts at: 387 2943 387
Size: 2556 5758
NgoMIV G'CCGG_C
Cuts at: 4212 4212
Size: 8314
NheI G'CTAG_C
Cuts at: 1052 2479 1052
Size: 1427 6887
NotI GC'GGCC_GC
Cuts at: 5761 5761
Size: 8314
NspI r_CATG'y
Cuts at: 1132 1229 2473 3368 3557 3604 5291 5672 7217
Size: 97 1244 895 189 47 1687 381 1545
Cuts at: 7217 1132
Size: 2229
Fragments arranged by size:
2229 1687 1545 1244 895 381 189 97
47
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8314
PciI A'CATG_T
Cuts at: 3364 3553 7213 3364
Size: 189 3660 4465
Fragments arranged by size:
4465 3660 189
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2087 2706 3340 3547 3961 4692 1108
Size: 979 619 634 207 414 731 4730
Fragments arranged by size:
4730 979 731 634 619 414 207
PmeI GTTT'AAAC
Cuts at: 5618 5618
Size: 8314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2303 2335 4038 4070 6383 6415 7896 7928 2303
Size: 32 1703 32 2313 32 1481 32 2689
Fragments arranged by size:
2689 2313 1703 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2821 2860 3374 3406 5047 5089 2821
Size: 39 514 32 1641 42 6046
Fragments arranged by size:
6046 1641 514 42 39 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8314
PsiI TTA'TAA
Cuts at: 4641 5884 4641
Size: 1243 7071
PspOMI G'GGCC_C
Cuts at: 2038 3510 4467 4884 2038
Size: 1472 957 417 5468
Fragments arranged by size:
5468 1472 957 417
PspXI vC'TCGA_Gb
Cuts at: 4251 4251
Size: 8314
PstI C_TGCA'G
Cuts at: 830 2877 3225 3351 3678 3777 3939 4308 4614
Size: 2047 348 126 327 99 162 369 306
Cuts at: 4614 4773 4836 5220 5666 830
Size: 159 63 384 446 3478
Fragments arranged by size:
3478 2047 446 384 369 348 327 306
162 159 126 99 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8314
PvuII CAG'CTG
Cuts at: 3190 3472 3778 4000 4240 4333 4630 4894 5008
Size: 282 306 222 240 93 297 264 114
Cuts at: 5008 5495 3190
Size: 487 6009
Fragments arranged by size:
6009 487 306 297 282 264 240 222
114 93
SacI G_AGCT'C
Cuts at: 2284 2291 3276 5022 5531 2284
Size: 7 985 1746 509 5067
Fragments arranged by size:
5067 1746 985 509 7
SacII CC_GC'GG
Cuts at: 2846 2846
Size: 8314
SalI G'TCGA_C
Cuts at: 1936 5656 1936
Size: 3720 4594
SbfI CC_TGCA'GG
Cuts at: 4773 5666 4773
Size: 893 7421
ScaI AGT'ACT
Cuts at: 6562 8274 6562
Size: 1712 6602
SexAI A'CCwGG_T
Cuts at: 4995 4995
Size: 8314
SfcI C'TryA_G
Cuts at: 826 1045 2873 3116 3221 3347 3674 3773 3935
Size: 219 1828 243 105 126 327 99 162
Cuts at: 3935 4304 4610 4769 4832 5186 5216 5597 5662
Size: 369 306 159 63 354 30 381 65
Cuts at: 5662 6797 7478 7669 826
Size: 1135 681 191 1471
Fragments arranged by size:
1828 1471 1135 681 381 369 354 327
306 243 219 191 162 159 126 105
99 65 63 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8314
SgrAI Cr'CCGG_yG
Cuts at: 1700 4212 1700
Size: 2512 5802
SmaI CCC'GGG
Cuts at: 1872 2036 2585 3422 5641 1872
Size: 164 549 837 2219 4545
Fragments arranged by size:
4545 2219 837 549 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3893 3926 4251 4904 5153 6418
Size: 197 926 1950 33 325 653 249 1265
Cuts at: 6418 7319 7581 820
Size: 901 262 1553
Fragments arranged by size:
1950 1553 1265 926 901 653 325 262
249 197 33
SnaBI TAC'GTA
Cuts at: 493 6105 493
Size: 5612 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8314
SphI G_CATG'C
Cuts at: 1132 2473 3604 5672 1132
Size: 1341 1131 2068 3774
Fragments arranged by size:
3774 2068 1341 1131
SspI AAT'ATT
Cuts at: 5 52 6238 5
Size: 47 6186 2081
Fragments arranged by size:
6186 2081 47
StuI AGG'CCT
Cuts at: 1517 2420 1517
Size: 903 7411
StyI C'CwwG_G
Cuts at: 513 1065 2004 2050 2266 4107 4686 5070 5753
Size: 552 939 46 216 1841 579 384 683
Cuts at: 5753 513
Size: 3074
Fragments arranged by size:
3074 1841 939 683 579 552 384 216
46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2032 4575 6341 6358 6511 6696 7141
Size: 1255 452 2543 1766 17 153 185 445
Cuts at: 7141 325
Size: 1498
Fragments arranged by size:
2543 1766 1498 1255 452 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6560 8272 96
Size: 274 80 33 51 1443 4583 1712 138
Fragments arranged by size:
4583 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5039 5071 8274 8306 5039
Size: 32 3203 32 5047
Fragments arranged by size:
5047 3203 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2819 5549 1316
Size: 1503 2730 4081
Fragments arranged by size:
4081 2730 1503
XbaI T'CTAG_A
Cuts at: 5650 5650
Size: 8314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3962 4114 4393 5233 3962
Size: 152 279 840 7043
Fragments arranged by size:
7043 840 279 152
XhoI C'TCGA_G
Cuts at: 1943 4251 4904 1943
Size: 2308 653 5353
Fragments arranged by size:
5353 2308 653
XmaI C'CCGG_G
Cuts at: 1870 2034 2583 3420 5639 1870
Size: 164 549 837 2219 4545
Fragments arranged by size:
4545 2219 837 549 164
XmnI GAAnn'nnTTC
Cuts at: 6443 6443
Size: 8314
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6122 276
Size: 53 83 186 936 4588 2468
Fragments arranged by size:
4588 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PsrI RsrII SanDI
SapI SfiI SfoI SrfI SwaI