Restriction map for
pFN21AA1291
(Circular) (Six-base) MAPSORT of: pFN21AA1291.seq Check: 4042 from: 1 to: 8317
pFN21AA1291 8317 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6127 278
Size: 53 83 186 936 4591 2468
Fragments arranged by size:
4591 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4179 5660 1937
Size: 2242 1481 4594
Fragments arranged by size:
4594 2242 1481
Acc65I G'GTAC_C
Cuts at: 5325 5638 5325
Size: 313 8004
AclI AA'CG_TT
Cuts at: 1563 6444 6817 1563
Size: 4881 373 3063
Fragments arranged by size:
4881 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 2294 2557 2777 2981 3124 6380 7764
Size: 560 102 263 220 204 143 3256 1384
Cuts at: 7764 8032 1632
Size: 268 1917
Fragments arranged by size:
3256 1917 1384 560 268 263 220 204
143 102
AfeI AGC'GCT
Cuts at: 2087 5085 2087
Size: 2998 5319
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8120
AflIII A'CryG_T
Cuts at: 1203 2221 7216 8193 1203
Size: 1018 4995 977 1327
Fragments arranged by size:
4995 1327 1018 977
AgeI A'CCGG_T
Cuts at: 7148 7148
Size: 8317
AhdI GACnn_n'nnGTC
Cuts at: 2716 7046 2716
Size: 4330 3987
AleI CACnn'nnGTG
Cuts at: 1166 4962 5228 1166
Size: 3796 266 4255
Fragments arranged by size:
4255 3796 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8317
AlwNI CAG_nnn'CTG
Cuts at: 2215 3026 4041 4312 6060 7632 2215
Size: 811 1015 271 1748 1572 2900
Fragments arranged by size:
2900 1748 1572 1015 811 271
ApaLI G'TGCA_C
Cuts at: 4158 6374 7530 4158
Size: 2216 1156 4945
Fragments arranged by size:
4945 2216 1156
ApoI r'AATT_y
Cuts at: 1493 3442 3579 5626 5852 6004 8113 1493
Size: 1949 137 2047 226 152 2109 1697
Fragments arranged by size:
2109 2047 1949 1697 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 5090 6871 160
Size: 872 4058 1781 1606
Fragments arranged by size:
4058 1781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8317
AvaI C'yCGr_G
Cuts at: 1870 1943 3321 3786 4902 5642 1870
Size: 73 1378 465 1116 740 4545
Fragments arranged by size:
4545 1378 1116 740 465 73
AvrII C'CTAG_G
Cuts at: 4555 4555
Size: 8317
BamHI G'GATC_C
Cuts at: 3364 3637 4138 5647 6014 3364
Size: 273 501 1509 367 5667
Fragments arranged by size:
5667 1509 501 367 273
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4024 5325 5638 7093 618
Size: 325 217 627 2237 1301 313 1455 1842
Fragments arranged by size:
2237 1842 1455 1301 627 325 313 217
BanII G_rGCy'C
Cuts at: 1391 3136 5636 1391
Size: 1745 2500 4072
Fragments arranged by size:
4072 2500 1745
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2633 2807 2856 3481 3715 4480 928
Size: 971 734 174 49 625 234 765 4765
Fragments arranged by size:
4765 971 765 734 625 234 174 49
BbvCI CC'TCA_GC
Cuts at: 5020 5020
Size: 8317
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6508 6542 1903
Size: 34 4571 34 3678
Fragments arranged by size:
4571 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4750 6209 7419 4750
Size: 1459 1210 5648
Fragments arranged by size:
5648 1459 1210
BclI T'GATC_A
Cuts at: 1548 2373 1548
Size: 825 7492
BfrBI ATG'CAT
Cuts at: 2893 2893
Size: 8317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4388 6928 136
Size: 107 122 71 631 605 2716 2540 1525
Fragments arranged by size:
2716 2540 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8312 8312
Size: 8317
BlpI GC'TnA_GC
Cuts at: 4627 5734 4627
Size: 1107 7210
Bme1580I G_kGCm'C
Cuts at: 1790 3946 4162 6378 7534 1790
Size: 2156 216 2216 1156 2573
Fragments arranged by size:
2573 2216 2156 1156 216
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8317
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2722 6048 7006
Size: 416 53 83 399 24 1305 3326 958
Cuts at: 7006 442
Size: 1753
Fragments arranged by size:
3326 1753 1305 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4382 1056
Size: 3326 4991
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4803 4803
Size: 8317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2777 3486 4154 4895 5351 5582 6977 1239
Size: 1538 709 668 741 456 231 1395 2579
Fragments arranged by size:
2579 1538 1395 741 709 668 456 231
Bpu10I CC'TnA_GC
Cuts at: 5020 5020
Size: 8317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 2179 4035 4848 6442 7307 7605 2090
Size: 89 1856 813 1594 865 298 2802
Fragments arranged by size:
2802 1856 1594 865 813 298 89
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6980 882
Size: 617 5481 2219
Fragments arranged by size:
5481 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5214 6108 493
Size: 711 4010 894 2702
Fragments arranged by size:
4010 2702 894 711
BsaBI GATnn'nnATC
Cuts at: 2468 8311 2468
Size: 5843 2474
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6124 6506 7141 275
Size: 53 83 186 936 4591 382 635 1451
Fragments arranged by size:
4591 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2853 6750 7148 7422 7569 7980 2853
Size: 3897 398 274 147 411 3190
Fragments arranged by size:
3897 3190 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 2252 2282 2750
Size: 30 165 30 657 30 162 30 468
Cuts at: 2750 2780 2922 2952 4127 4157 1178
Size: 30 142 30 1175 30 5338
Fragments arranged by size:
5338 1175 657 468 165 162 142 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2342 2943 3307 3820 3893 4028 4892
Size: 946 210 601 364 513 73 135 864
Cuts at: 4892 5159 1186
Size: 267 4344
Fragments arranged by size:
4344 946 864 601 513 364 267 210
135 73
BseYI C'CCAG_C
Cuts at: 2082 2989 3013 3389 3852 4241 4614 4972 7520
Size: 907 24 376 463 389 373 358 2548
Cuts at: 7520 8016 2082
Size: 496 2383
Fragments arranged by size:
2548 2383 907 496 463 389 376 373
358 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3039 3527 3660 4282 1163
Size: 573 1303 488 133 622 5198
Fragments arranged by size:
5198 1303 622 573 488 133
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5767 6528 7556 1540
Size: 275 186 3766 761 1028 2301
Fragments arranged by size:
3766 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3328 4162 4707 4725 4969 6378 6463 7534 3328
Size: 834 545 18 244 1409 85 1071 4111
Fragments arranged by size:
4111 1409 1071 834 545 244 85 18
BsmI GAATG_Cn'
Cuts at: 4185 4515 5828 5921 4185
Size: 330 1313 93 6581
Fragments arranged by size:
6581 1313 330 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4977 5558 4977
Size: 581 7736
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3136 3328 3946 4162 4707 4725 4969
Size: 399 1346 192 618 216 545 18 244
Cuts at: 4969 5636 6378 6463 7534 1391
Size: 667 742 85 1071 2174
Fragments arranged by size:
2174 1346 1071 742 667 618 545 399
244 216 192 85 18
BspEI T'CCGG_A
Cuts at: 7980 7980
Size: 8317
BspHI T'CATG_A
Cuts at: 2061 6206 7936 2061
Size: 4145 1730 2442
Fragments arranged by size:
4145 2442 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5672 844
Size: 4828 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6204 1394
Size: 429 4381 3507
Fragments arranged by size:
4381 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2820 3008 5463 6812 6986 65
Size: 1183 1572 188 2455 1349 174 1396
Fragments arranged by size:
2455 1572 1396 1349 1183 188 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 6961 7148 1700
Size: 110 281 4870 187 2869
Fragments arranged by size:
4870 2869 281 187 110
BsrGI T'GTAC_A
Cuts at: 96 5078 96
Size: 4982 3335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8317
BssSI C'ACGA_G
Cuts at: 6377 7389 6377
Size: 1012 7305
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2918 4066 794
Size: 2124 1148 5045
Fragments arranged by size:
5045 2124 1148
BstBI TT'CG_AA
Cuts at: 3559 7121 3559
Size: 3562 4755
BstEII G'GTnAC_C
Cuts at: 1184 5011 1184
Size: 3827 4490
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2397 3389 4411 5329 2011
Size: 386 992 1022 918 4999
Fragments arranged by size:
4999 1022 992 918 386
BstYI r'GATC_y
Cuts at: 1972 2211 2568 3364 3637 4138 5647 6014 6399
Size: 239 357 796 273 501 1509 367 385
Cuts at: 6399 6416 7868 7954 7966 8312 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1509 1452 796 501 385 367 357
346 273 239 86 17 12
BstZ17I GTA'TAC
Cuts at: 4180 4180
Size: 8317
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6826
Fragments arranged by size:
6826 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2024 3303 487
Size: 683 56 798 1279 5501
Fragments arranged by size:
5501 1279 798 683 56
BtsI GCAGTG_nn'
Cuts at: 2733 3515 3798 5192 5312 5833 6627 6647 2733
Size: 782 283 1394 120 521 794 20 4403
Fragments arranged by size:
4403 1394 794 782 521 283 120 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5590 5625 529
Size: 35 5026 35 3221
Fragments arranged by size:
5026 3221 35 35
DraI TTT'AAA
Cuts at: 2125 5406 5621 2125
Size: 3281 215 4821
Fragments arranged by size:
4821 3281 215
DraIII CAC_nnn'GTG
Cuts at: 4066 4066
Size: 8317
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4484 7324 809
Size: 507 3168 2840 1802
Fragments arranged by size:
3168 2840 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2387 4032 5001
Size: 54 1425 50 275 18 557 1645 969
Cuts at: 5001 5598 5764 6653 8282 8
Size: 597 166 889 1629 43
Fragments arranged by size:
1645 1629 1425 969 889 597 557 275
166 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5764 1537
Size: 275 3952 4090
Fragments arranged by size:
4090 3952 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 2957 3115 3143 4109 4224 4712 6247
Size: 966 638 158 28 966 115 488 1535
Cuts at: 6247 1353
Size: 3423
Fragments arranged by size:
3423 1535 966 966 638 488 158 115
28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2896 6904 7278 7424 387
Size: 1064 138 1307 4008 374 146 1280
Fragments arranged by size:
4008 1307 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2294 2557 2777 2981 3124 3486
Size: 393 560 102 263 220 204 143 362
Cuts at: 3486 4154 4895 5351 5582 6380 6977 7764 8032
Size: 668 741 456 231 798 597 787 268
Cuts at: 8032 1239
Size: 1524
Fragments arranged by size:
1524 798 787 741 668 597 560 456
393 362 268 263 231 220 204 143
102
EcoNI CCTnn'n_nnAGG
Cuts at: 3716 4229 4559 5137 3716
Size: 513 330 578 6896
Fragments arranged by size:
6896 578 513 330
EcoO109I rG'GnC_Cy
Cuts at: 3775 4568 4603 3775
Size: 793 35 7489
Fragments arranged by size:
7489 793 35
EcoRI G'AATT_C
Cuts at: 3442 5626 6004 3442
Size: 2184 378 5755
Fragments arranged by size:
5755 2184 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3247 3247
Size: 8317
FspI TGC'GCA
Cuts at: 6823 6823
Size: 8317
HaeII r_GCGC'y
Cuts at: 1678 2089 5087 5440 7464 1678
Size: 411 2998 353 2024 2531
Fragments arranged by size:
2998 2531 2024 411 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2336 2368 2702
Size: 32 135 32 488 32 244 32 334
Cuts at: 2702 2734 2956 2988 5114 5146 6958 6990 7032
Size: 32 222 32 2126 32 1812 32 42
Cuts at: 7032 7064 1373
Size: 32 2626
Fragments arranged by size:
2626 2126 1812 488 334 244 222 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4639 4899 5661 5907 669
Size: 988 281 2701 260 762 246 3079
Fragments arranged by size:
3079 2701 988 762 281 260 246
HindIII A'AGCT_T
Cuts at: 748 5399 748
Size: 4651 3666
HpaI GTT'AAC
Cuts at: 4899 5907 4899
Size: 1008 7309
KpnI G_GTAC'C
Cuts at: 5329 5642 5329
Size: 313 8004
MfeI C'AATT_G
Cuts at: 5916 5916
Size: 8317
MluI A'CGCG_T
Cuts at: 8193 8193
Size: 8317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2826 3439 7431 7615 1305
Size: 1521 613 3992 184 2007
Fragments arranged by size:
3992 2007 1521 613 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2389 4034 5003 5600 10
Size: 54 1425 343 557 1645 969 597 2727
Fragments arranged by size:
2727 1645 1425 969 597 557 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3551 3833 4962 5070 5228 6275
Size: 648 843 1542 282 1129 108 158 1047
Cuts at: 6275 6634 6793 518
Size: 359 159 2042
Fragments arranged by size:
2042 1542 1129 1047 843 648 359 282
159 158 108
MspA1I CmG'CkG
Cuts at: 1606 2041 2807 3001 5733 6410 7558 7803 1606
Size: 435 766 194 2732 677 1148 245 2120
Fragments arranged by size:
2732 2120 1148 766 677 435 245 194
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6826
Fragments arranged by size:
6826 939 552
NdeI CA'TA_TG
Cuts at: 387 4776 387
Size: 4389 3928
NheI G'CTAG_C
Cuts at: 1052 4378 1052
Size: 3326 4991
NotI GC'GGCC_GC
Cuts at: 5764 5764
Size: 8317
NsiI A_TGCA'T
Cuts at: 2895 2895
Size: 8317
NspI r_CATG'y
Cuts at: 1132 1229 2251 2410 3357 3815 3880 4060 5675
Size: 97 1022 159 947 458 65 180 1615
Cuts at: 5675 7220 1132
Size: 1545 2229
Fragments arranged by size:
2229 1615 1545 1022 947 458 180 159
97 65
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8317
PciI A'CATG_T
Cuts at: 7216 7216
Size: 8317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 4041 4979 1108
Size: 1452 1481 938 4446
Fragments arranged by size:
4446 1481 1452 938
PfoI T'CCnGG_A
Cuts at: 2214 2754 2214
Size: 540 7777
PmeI GTTT'AAAC
Cuts at: 5621 5621
Size: 8317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6386 6418 7899 7931 6386
Size: 32 1481 32 6772
Fragments arranged by size:
6772 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4603 4603
Size: 8317
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8317
PsiI TTA'TAA
Cuts at: 3885 4343 5887 3885
Size: 458 1544 6315
Fragments arranged by size:
6315 1544 458
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4339 4371 4339
Size: 32 8285
PstI C_TGCA'G
Cuts at: 830 3457 4846 5421 5669 830
Size: 2627 1389 575 248 3478
Fragments arranged by size:
3478 2627 1389 575 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8317
PvuII CAG'CTG
Cuts at: 2041 3001 2041
Size: 960 7357
SalI G'TCGA_C
Cuts at: 1936 5659 1936
Size: 3723 4594
SapI GCTCTTCn'nnn_
Cuts at: 3115 4109 4712 3115
Size: 994 603 6720
Fragments arranged by size:
6720 994 603
SbfI CC_TGCA'GG
Cuts at: 5669 5669
Size: 8317
ScaI AGT'ACT
Cuts at: 6565 8277 6565
Size: 1712 6605
SfcI C'TryA_G
Cuts at: 826 1045 3453 3697 4842 5417 5665 6800 7481
Size: 219 2408 244 1145 575 248 1135 681
Cuts at: 7481 7672 826
Size: 191 1471
Fragments arranged by size:
2408 1471 1145 1135 681 575 248 244
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8317
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8317
SmaI CCC'GGG
Cuts at: 1872 3788 5644 1872
Size: 1916 1856 4545
Fragments arranged by size:
4545 1916 1856
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 2158 4050 4863 6421 7322
Size: 197 926 162 53 1892 813 1558 901
Cuts at: 7322 7584 820
Size: 262 1553
Fragments arranged by size:
1892 1558 1553 926 901 813 262 197
162 53
SnaBI TAC'GTA
Cuts at: 493 6108 493
Size: 5615 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8317
SphI G_CATG'C
Cuts at: 1132 3815 5675 1132
Size: 2683 1860 3774
Fragments arranged by size:
3774 2683 1860
SspI AAT'ATT
Cuts at: 5 52 3266 4651 6241 5
Size: 47 3214 1385 1590 2081
Fragments arranged by size:
3214 2081 1590 1385 47
StuI AGG'CCT
Cuts at: 1517 4131 1517
Size: 2614 5703
StyI C'CwwG_G
Cuts at: 513 1065 2004 3633 4404 4555 5487 5756 513
Size: 552 939 1629 771 151 932 269 3074
Fragments arranged by size:
3074 1629 939 932 771 552 269 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2096 2704 3005 3563 4181 6344 6361
Size: 1255 516 608 301 558 618 2163 17
Cuts at: 6361 6514 6699 7144 325
Size: 153 185 445 1498
Fragments arranged by size:
2163 1498 1255 618 608 558 516 445
301 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5078 6563 8275
Size: 274 80 33 51 1443 3101 1485 1712
Cuts at: 8275 96
Size: 138
Fragments arranged by size:
3101 1712 1485 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2750 2782 3073 3105 4127 4159 4239 4271 8277
Size: 32 291 32 1022 32 80 32 4006
Cuts at: 8277 8309 2750
Size: 32 2758
Fragments arranged by size:
4006 2758 1022 291 80 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8317
XbaI T'CTAG_A
Cuts at: 5653 5653
Size: 8317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3214 3214
Size: 8317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8317
XmaI C'CCGG_G
Cuts at: 1870 3786 5642 1870
Size: 1916 1856 4545
Fragments arranged by size:
4545 1916 1856
XmnI GAAnn'nnTTC
Cuts at: 3069 3579 4004 6446 3069
Size: 510 425 2442 4940
Fragments arranged by size:
4940 2442 510 425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6125 276
Size: 53 83 186 936 4591 2468
Fragments arranged by size:
4591 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BsiWI Bsu36I
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI