Restriction map for
pFN21AA1286
(Circular) (Six-base) MAPSORT of: pFN21AA1286.seq Check: 3218 from: 1 to: 8320
pFN21AA1286 8320 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4140 5533 4140
Size: 1393 6927
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6130 278
Size: 53 83 186 936 4594 2468
Fragments arranged by size:
4594 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2133 2568 5663 1937
Size: 196 435 3095 4594
Fragments arranged by size:
4594 3095 435 196
Acc65I G'GTAC_C
Cuts at: 4754 5641 4754
Size: 887 7433
AclI AA'CG_TT
Cuts at: 1563 6447 6820 1563
Size: 4884 373 3063
Fragments arranged by size:
4884 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2211 2460 2916 3263 3671 4259 5246 5381
Size: 579 249 456 347 408 588 987 135
Cuts at: 5381 6383 7767 8035 1632
Size: 1002 1384 268 1917
Fragments arranged by size:
1917 1384 1002 987 588 579 456 408
347 268 249 135
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8123
AflIII A'CryG_T
Cuts at: 1203 3056 7219 8196 1203
Size: 1853 4163 977 1327
Fragments arranged by size:
4163 1853 1327 977
AgeI A'CCGG_T
Cuts at: 7151 7151
Size: 8320
AhdI GACnn_n'nnGTC
Cuts at: 7049 7049
Size: 8320
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4148 5555 1184
Size: 2964 1407 3949
Fragments arranged by size:
3949 2964 1407
AlwNI CAG_nnn'CTG
Cuts at: 4348 4944 5390 6063 7635 4348
Size: 596 446 673 1572 5033
Fragments arranged by size:
5033 1572 673 596 446
ApaI G_GGCC'C
Cuts at: 2617 2869 4216 4294 4422 5592 2617
Size: 252 1347 78 128 1170 5345
Fragments arranged by size:
5345 1347 1170 252 128 78
ApaLI G'TGCA_C
Cuts at: 3060 6377 7533 3060
Size: 3317 1156 3847
Fragments arranged by size:
3847 3317 1156
ApoI r'AATT_y
Cuts at: 1493 3887 5629 5855 6007 8116 1493
Size: 2394 1742 226 152 2109 1697
Fragments arranged by size:
2394 2109 1742 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4590 6874 160
Size: 872 3558 2284 1606
Fragments arranged by size:
3558 2284 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8320
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 4115 4136 5133 5304 5645 1870
Size: 73 145 2027 21 997 171 341 4545
Fragments arranged by size:
4545 2027 997 341 171 145 73 21
BamHI G'GATC_C
Cuts at: 2889 3829 5650 6017 2889
Size: 940 1821 367 5192
Fragments arranged by size:
5192 1821 940 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2834 3119 4754 5641 7096
Size: 325 217 627 1047 285 1635 887 1455
Cuts at: 7096 618
Size: 1842
Fragments arranged by size:
1842 1635 1455 1047 887 627 325 285
217
BanII G_rGCy'C
Cuts at: 1391 2617 2869 3078 3096 4216 4294 4422 4524
Size: 1226 252 209 18 1120 78 128 102
Cuts at: 4524 4572 4650 5592 5639 1391
Size: 48 78 942 47 4072
Fragments arranged by size:
4072 1226 1120 942 252 209 128 102
78 78 48 47 18
BbeI G_GCGC'C
Cuts at: 2838 2838
Size: 8320
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2448 2643 3632 4161 4185 4476 5217
Size: 971 549 195 989 529 24 291 741
Cuts at: 5217 5487 928
Size: 270 3761
Fragments arranged by size:
3761 989 971 741 549 529 291 270
195 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6511 6545 1903
Size: 34 4574 34 3678
Fragments arranged by size:
4574 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4405 6212 7422 4405
Size: 1807 1210 5303
Fragments arranged by size:
5303 1807 1210
BclI T'GATC_A
Cuts at: 1548 5283 1548
Size: 3735 4585
BfrBI ATG'CAT
Cuts at: 2324 2324
Size: 8320
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3117 4417 4528
Size: 107 122 71 631 605 1445 1300 111
Cuts at: 4528 6931 136
Size: 2403 1525
Fragments arranged by size:
2403 1525 1445 1300 631 605 122 111
107 71
BglII A'GATC_T
Cuts at: 3462 8315 3462
Size: 4853 3467
BlpI GC'TnA_GC
Cuts at: 3603 5737 3603
Size: 2134 6186
Bme1580I G_kGCm'C
Cuts at: 1790 2532 2617 2862 2869 3064 3339 4127 4216
Size: 742 85 245 7 195 275 788 89
Cuts at: 4216 4294 4422 5142 5592 6381 7537 1790
Size: 78 128 720 450 789 1156 2573
Fragments arranged by size:
2573 1156 789 788 742 720 450 275
245 195 128 89 85 78 7
BmgBI CAC'GTC
Cuts at: 1318 4183 5356 1318
Size: 2865 1173 4282
Fragments arranged by size:
4282 2865 1173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2872 4536 6051
Size: 416 53 83 399 24 1455 1664 1515
Cuts at: 6051 7009 442
Size: 958 1753
Fragments arranged by size:
1753 1664 1515 1455 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8320
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2031 3178 3743 4143 4933 5417 2031
Size: 1147 565 400 790 484 4934
Fragments arranged by size:
4934 1147 790 565 484 400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3791 3953 4271 4463 4538 4868 4875 5412
Size: 2552 162 318 192 75 330 7 537
Cuts at: 5412 6980 1239
Size: 1568 2579
Fragments arranged by size:
2579 2552 1568 537 330 318 192 162
75 7
Bpu10I CC'TnA_GC
Cuts at: 5182 5573 5182
Size: 391 7929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 2597 3288 3311 3883 5483 6445 7310 7608
Size: 540 691 23 572 1600 962 865 298
Cuts at: 7608 2057
Size: 2769
Fragments arranged by size:
2769 1600 962 865 691 572 540 298
23
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2087 3479 5060 5214 6983 882
Size: 617 588 1392 1581 154 1769 2219
Fragments arranged by size:
2219 1769 1581 1392 617 588 154
BsaAI yAC'GTr
Cuts at: 493 1204 6111 493
Size: 711 4907 2702
Fragments arranged by size:
4907 2702 711
BsaBI GATnn'nnATC
Cuts at: 8314 8314
Size: 8320
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2146 2739 2835 5096
Size: 53 83 186 936 613 593 96 2261
Cuts at: 5096 6127 6509 7144 275
Size: 1031 382 635 1451
Fragments arranged by size:
2261 1451 1031 936 635 613 593 382
186 96 83 53
BsaWI w'CCGG_w
Cuts at: 6753 7151 7425 7572 7983 6753
Size: 398 274 147 411 7090
Fragments arranged by size:
7090 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3392 3422 3638 3668 4154
Size: 30 165 30 1989 30 216 30 486
Cuts at: 4154 4184 4933 4963 5228 5258 1178
Size: 30 749 30 265 30 4240
Fragments arranged by size:
4240 1989 749 486 265 216 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2492 3275 3659 3974 3983 4475 4494 1186
Size: 1306 783 384 315 9 492 19 5012
Fragments arranged by size:
5012 1306 783 492 384 315 19 9
BseYI C'CCAG_C
Cuts at: 2115 2354 3071 4974 5365 7523 8019 2115
Size: 239 717 1903 391 2158 496 2416
Fragments arranged by size:
2416 2158 1903 717 496 391 239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3027 3654 3819 4253 4518 5303 5561
Size: 573 1291 627 165 434 265 785 258
Cuts at: 5561 1163
Size: 3922
Fragments arranged by size:
3922 1291 785 627 573 434 265 258
165
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4752 5770 6531 7559 1540
Size: 275 186 2751 1018 761 1028 2301
Fragments arranged by size:
2751 2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2081 3064 4650 6381 6466 7537 2081
Size: 983 1586 1731 85 1071 2864
Fragments arranged by size:
2864 1731 1586 1071 983 85
BsmI GAATG_Cn'
Cuts at: 2041 2228 3044 5831 5924 2041
Size: 187 816 2787 93 4437
Fragments arranged by size:
4437 2787 816 187 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2532 2617 2862 2869 3064 3078
Size: 399 291 451 85 245 7 195 14
Cuts at: 3078 3096 3339 4127 4216 4294 4422 4524 4572
Size: 18 243 788 89 78 128 102 48
Cuts at: 4572 4650 5142 5592 5639 6381 6466 7537 1391
Size: 78 492 450 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 788 742 492 451 450 399
291 245 243 195 128 102 89 85
85 78 78 48 47 18 14 7
BspEI T'CCGG_A
Cuts at: 7983 7983
Size: 8320
BspHI T'CATG_A
Cuts at: 3081 6209 7939 3081
Size: 3128 1730 3462
Fragments arranged by size:
3462 3128 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2984 3730 3822 4140 4602 5533 5675 844
Size: 2140 746 92 318 462 931 142 3489
Fragments arranged by size:
3489 2140 931 746 462 318 142 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3685 6207 1394
Size: 429 1862 2522 3507
Fragments arranged by size:
3507 2522 1862 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2689 6815 6989 65
Size: 1183 1441 4126 174 1396
Fragments arranged by size:
4126 1441 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 2571 4751 6964 7151 1700
Size: 110 236 525 2180 2213 187 2869
Fragments arranged by size:
2869 2213 2180 525 236 187 110
BsrGI T'GTAC_A
Cuts at: 96 2719 96
Size: 2623 5697
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8320
BssSI C'ACGA_G
Cuts at: 6380 7392 6380
Size: 1012 7308
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2817 4453 4696 794
Size: 2023 1636 243 4418
Fragments arranged by size:
4418 2023 1636 243
BstBI TT'CG_AA
Cuts at: 7124 7124
Size: 8320
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8320
BstXI CCAn_nnnn'nTGG
Cuts at: 3769 3904 4633 5617 3769
Size: 135 729 984 6472
Fragments arranged by size:
6472 984 729 135
BstYI r'GATC_y
Cuts at: 1972 2889 3462 3829 5650 6017 6402 6419 7871
Size: 917 573 367 1821 367 385 17 1452
Cuts at: 7871 7957 7969 8315 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1821 1452 917 573 385 367 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2754 2754
Size: 8320
BtgI C'CryG_G
Cuts at: 513 1065 3762 4864 5113 5215 5487 513
Size: 552 2697 1102 249 102 272 3346
Fragments arranged by size:
3346 2697 1102 552 272 249 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3243 5576 487
Size: 683 56 2017 2333 3231
Fragments arranged by size:
3231 2333 2017 683 56
BtsI GCAGTG_nn'
Cuts at: 2614 3180 3642 4664 5092 5447 5836 6630 6650
Size: 566 462 1022 428 355 389 794 20
Cuts at: 6650 2614
Size: 4284
Fragments arranged by size:
4284 1022 794 566 462 428 389 355
20
ClaI AT'CG_AT
Cuts at: 1554 2631 5289 1554
Size: 1077 2658 4585
Fragments arranged by size:
4585 2658 1077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2812 2847 529
Size: 35 2248 35 6002
Fragments arranged by size:
6002 2248 35 35
DraI TTT'AAA
Cuts at: 5624 5624
Size: 8320
DraIII CAC_nnn'GTG
Cuts at: 3173 4766 3173
Size: 1593 6727
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7327 809
Size: 507 6011 1802
Fragments arranged by size:
6011 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3177 4749 4928
Size: 54 1425 50 275 18 1347 1572 179
Cuts at: 4928 5767 6656 8285 8
Size: 839 889 1629 43
Fragments arranged by size:
1629 1572 1425 1347 889 839 275 179
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4749 5767 1537
Size: 275 2937 1018 4090
Fragments arranged by size:
4090 2937 1018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2699 3083 3264 3270 3285 3710 4799 4902
Size: 1346 384 181 6 15 425 1089 103
Cuts at: 4902 4980 5408 5420 6250 1353
Size: 78 428 12 830 3423
Fragments arranged by size:
3423 1346 1089 830 428 425 384 181
103 78 15 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3940 4034 4298 4497 6907 7281
Size: 1064 138 2351 94 264 199 2410 374
Cuts at: 7281 7427 387
Size: 146 1280
Fragments arranged by size:
2410 2351 1280 1064 374 264 199 146
138 94
EcoICRI GAG'CTC
Cuts at: 4648 4648
Size: 8320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2460 2916 3263 3671 3791 3953
Size: 393 579 249 456 347 408 120 162
Cuts at: 3953 4259 4271 4463 4538 4868 4875 5246 5381
Size: 306 12 192 75 330 7 371 135
Cuts at: 5381 5412 6383 6980 7767 8035 1239
Size: 31 971 597 787 268 1524
Fragments arranged by size:
1524 971 787 597 579 456 408 393
371 347 330 306 268 249 192 162
135 120 75 31 12 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3448 3484 3818 3448
Size: 36 334 7950
Fragments arranged by size:
7950 334 36
EcoO109I rG'GnC_Cy
Cuts at: 2482 2613 4054 4290 4418 4419 4843 4968 5311
Size: 131 1441 236 128 1 424 125 343
Cuts at: 5311 5588 5589 2482
Size: 277 1 5213
Fragments arranged by size:
5213 1441 424 343 277 236 131 128
125 1 1
EcoRI G'AATT_C
Cuts at: 3887 5629 6007 3887
Size: 1742 378 6200
Fragments arranged by size:
6200 1742 378
EcoRV GAT'ATC
Cuts at: 2215 2215
Size: 8320
FspI TGC'GCA
Cuts at: 3137 6826 3137
Size: 3689 4631
HaeII r_GCGC'y
Cuts at: 1678 2838 3571 4275 4915 7467 1678
Size: 1160 733 704 640 2552 2531
Fragments arranged by size:
2552 2531 1160 733 704 640
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2570 2602 3392 3424 3726
Size: 32 135 32 998 32 790 32 302
Cuts at: 3726 3758 4026 4053 4058 4085 4541 4573 4713
Size: 32 268 27 5 27 456 32 140
Cuts at: 4713 4745 5275 5307 6961 6993 7035 7067 1373
Size: 32 530 32 1654 32 42 32 2626
Fragments arranged by size:
2626 1654 998 790 530 456 302 268
140 135 42 32 32 32 32 32
32 32 32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3859 5664 5910 669
Size: 988 281 1921 1805 246 3079
Fragments arranged by size:
3079 1921 1805 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5079 748
Size: 4331 3989
HpaI GTT'AAC
Cuts at: 5910 5910
Size: 8320
KasI G'GCGC_C
Cuts at: 2834 2834
Size: 8320
KpnI G_GTAC'C
Cuts at: 4758 5645 4758
Size: 887 7433
MfeI C'AATT_G
Cuts at: 5919 5919
Size: 8320
MluI A'CGCG_T
Cuts at: 8196 8196
Size: 8320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2000 7434 7618 1305
Size: 695 5434 184 2007
Fragments arranged by size:
5434 2007 695 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6498
Fragments arranged by size:
6498 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6278 6637 6796 518
Size: 648 5112 359 159 2042
Fragments arranged by size:
5112 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2401 2775 2964 3067 3508 4040 4237
Size: 513 282 374 189 103 441 532 197
Cuts at: 4237 4436 5217 5350 5736 6413 7561 7806 1606
Size: 199 781 133 386 677 1148 245 2120
Fragments arranged by size:
2120 1148 781 677 532 513 441 386
374 282 245 199 197 189 133 103
NaeI GCC'GGC
Cuts at: 2048 2048
Size: 8320
NarI GG'CG_CC
Cuts at: 2835 2835
Size: 8320
NcoI C'CATG_G
Cuts at: 513 1065 3762 4864 513
Size: 552 2697 1102 3969
Fragments arranged by size:
3969 2697 1102 552
NdeI CA'TA_TG
Cuts at: 387 2104 387
Size: 1717 6603
NgoMIV G'CCGG_C
Cuts at: 2046 2046
Size: 8320
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8320
NotI GC'GGCC_GC
Cuts at: 5767 5767
Size: 8320
NsiI A_TGCA'T
Cuts at: 2326 2326
Size: 8320
NspI r_CATG'y
Cuts at: 1132 1229 2324 3060 5678 7223 1132
Size: 97 1095 736 2618 1545 2229
Fragments arranged by size:
2618 2229 1545 1095 736 97
PasI CC'CwG_GG
Cuts at: 1348 4423 1348
Size: 3075 5245
PciI A'CATG_T
Cuts at: 3056 7219 3056
Size: 4163 4157
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4766 4870 1108
Size: 3658 104 4558
Fragments arranged by size:
4558 3658 104
PfoI T'CCnGG_A
Cuts at: 4845 4919 5145 4845
Size: 74 226 8020
Fragments arranged by size:
8020 226 74
PmeI GTTT'AAAC
Cuts at: 5624 5624
Size: 8320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5150 5182 6389 6421 7902 7934 5150
Size: 32 1207 32 1481 32 5536
Fragments arranged by size:
5536 1481 1207 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2482 4054 4843 4968 2482
Size: 1572 789 125 5834
Fragments arranged by size:
5834 1572 789 125
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8320
PsiI TTA'TAA
Cuts at: 5890 5890
Size: 8320
PspOMI G'GGCC_C
Cuts at: 2613 2865 4212 4290 4418 5588 2613
Size: 252 1347 78 128 1170 5345
Fragments arranged by size:
5345 1347 1170 252 128 78
PstI C_TGCA'G
Cuts at: 830 2548 2787 3588 3822 4671 5672 830
Size: 1718 239 801 234 849 1001 3478
Fragments arranged by size:
3478 1718 1001 849 801 239 234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8320
PvuII CAG'CTG
Cuts at: 4040 4237 4436 4040
Size: 197 199 7924
Fragments arranged by size:
7924 199 197
SacI G_AGCT'C
Cuts at: 4650 4650
Size: 8320
SacII CC_GC'GG
Cuts at: 5218 5218
Size: 8320
SalI G'TCGA_C
Cuts at: 1936 5662 1936
Size: 3726 4594
SapI GCTCTTCn'nnn_
Cuts at: 3083 3285 4902 5408 3083
Size: 202 1617 506 5995
Fragments arranged by size:
5995 1617 506 202
SbfI CC_TGCA'GG
Cuts at: 5672 5672
Size: 8320
ScaI AGT'ACT
Cuts at: 6568 8280 6568
Size: 1712 6608
SexAI A'CCwGG_T
Cuts at: 3169 4263 5616 3169
Size: 1094 1353 5873
Fragments arranged by size:
5873 1353 1094
SfcI C'TryA_G
Cuts at: 826 1045 2065 2544 2783 3466 3584 3818 4667
Size: 219 1020 479 239 683 118 234 849
Cuts at: 4667 4823 4945 5668 6803 7484 7675 826
Size: 156 122 723 1135 681 191 1471
Fragments arranged by size:
1471 1135 1020 849 723 683 681 479
239 234 219 191 156 122 118
SfoI GGC'GCC
Cuts at: 2836 2836
Size: 8320
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8320
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8320
SmaI CCC'GGG
Cuts at: 1872 2090 5306 5647 1872
Size: 218 3216 341 4545
Fragments arranged by size:
4545 3216 341 218
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2072 2576 3303 3326 3862 5133
Size: 197 926 129 504 727 23 536 1271
Cuts at: 5133 5498 6424 7325 7587 820
Size: 365 926 901 262 1553
Fragments arranged by size:
1553 1271 926 926 901 727 536 504
365 262 197 129 23
SnaBI TAC'GTA
Cuts at: 493 6111 493
Size: 5618 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8320
SphI G_CATG'C
Cuts at: 1132 2324 5678 1132
Size: 1192 3354 3774
Fragments arranged by size:
3774 3354 1192
SspI AAT'ATT
Cuts at: 5 52 6244 5
Size: 47 6192 2081
Fragments arranged by size:
6192 2081 47
StuI AGG'CCT
Cuts at: 1517 4451 1517
Size: 2934 5386
StyI C'CwwG_G
Cuts at: 513 1065 2289 3762 3954 4864 4931 5232 5450
Size: 552 1224 1473 192 910 67 301 218
Cuts at: 5450 5469 5759 513
Size: 19 290 3074
Fragments arranged by size:
3074 1473 1224 910 552 301 290 218
192 67 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2098 4159 5462 6347 6364 6517 6702
Size: 1255 518 2061 1303 885 17 153 185
Cuts at: 6702 7147 325
Size: 445 1498
Fragments arranged by size:
2061 1498 1303 1255 885 518 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2719 6566 8278
Size: 274 80 33 51 1443 742 3847 1712
Cuts at: 8278 96
Size: 138
Fragments arranged by size:
3847 1712 1443 742 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4933 4965 5228 5260 8280 8312 4933
Size: 32 263 32 3020 32 4941
Fragments arranged by size:
4941 3020 263 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4841 5459 1316
Size: 3525 618 4177
Fragments arranged by size:
4177 3525 618
XbaI T'CTAG_A
Cuts at: 5656 5656
Size: 8320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2362 2732 2362
Size: 370 7950
XhoI C'TCGA_G
Cuts at: 1943 5133 1943
Size: 3190 5130
XmaI C'CCGG_G
Cuts at: 1870 2088 5304 5645 1870
Size: 218 3216 341 4545
Fragments arranged by size:
4545 3216 341 218
XmnI GAAnn'nnTTC
Cuts at: 5402 6449 5402
Size: 1047 7273
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6128 276
Size: 53 83 186 936 4594 2468
Fragments arranged by size:
4594 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BsiWI BsmBI
BstZ17I FalI FseI FspAI NruI PacI PmlI PspXI
PsrI RsrII SanDI SfiI SrfI SwaI