Restriction map for
pFN21AA1271
(Circular) (Six-base) MAPSORT of: pFN21AA1271.seq Check: 7148 from: 1 to: 6325
pFN21AA1271 6325 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2147 2377 2618 2881 3469 2147
Size: 230 241 263 588 5003
Fragments arranged by size:
5003 588 263 241 230
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2277 3288 3668 1937
Size: 340 1011 380 4594
Fragments arranged by size:
4594 1011 380 340
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2037 2174 4388 5772 6040 1632
Size: 405 137 2214 1384 268 1917
Fragments arranged by size:
2214 1917 1384 405 268 137
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6128
AflIII A'CryG_T
Cuts at: 1203 5224 6201 1203
Size: 4021 977 1327
Fragments arranged by size:
4021 1327 977
AgeI A'CCGG_T
Cuts at: 5156 5156
Size: 6325
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 3116 1166
Size: 1950 4375
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6325
AlwNI CAG_nnn'CTG
Cuts at: 2692 2801 2957 3439 4068 5640 2692
Size: 109 156 482 629 1572 3377
Fragments arranged by size:
3377 1572 629 482 156 109
ApaI G_GGCC'C
Cuts at: 2361 3357 2361
Size: 996 5329
ApaLI G'TGCA_C
Cuts at: 4382 5538 4382
Size: 1156 5169
ApoI r'AATT_y
Cuts at: 1493 2470 3634 3860 4012 6121 1493
Size: 977 1164 226 152 2109 1697
Fragments arranged by size:
2109 1697 1164 977 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4879 160
Size: 872 3847 1606
Fragments arranged by size:
3847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 3301 3650 1870
Size: 73 1358 349 4545
Fragments arranged by size:
4545 1358 349 73
BamHI G'GATC_C
Cuts at: 3405 3655 4022 3405
Size: 250 367 5708
Fragments arranged by size:
5708 367 250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2449 2756 2869 3079 3191
Size: 325 217 627 662 307 113 210 112
Cuts at: 3191 3395 3520 3646 5101 618
Size: 204 125 126 1455 1842
Fragments arranged by size:
1842 1455 662 627 325 307 217 210
204 126 125 113 112
BanII G_rGCy'C
Cuts at: 1391 2361 3357 3550 3644 1391
Size: 970 996 193 94 4072
Fragments arranged by size:
4072 996 970 193 94
BbeI G_GCGC'C
Cuts at: 2453 3195 2453
Size: 742 5583
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 928
Size: 971 126 5228
Fragments arranged by size:
5228 971 126
BbvCI CC'TCA_GC
Cuts at: 2564 2564
Size: 6325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3302 3336 4516 4550 1903
Size: 34 50 34 1281 34 1180 34 3678
Fragments arranged by size:
3678 1281 1180 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4217 5427 4217
Size: 1210 5115
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6325
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4936 136
Size: 107 122 71 631 605 3264 1525
Fragments arranged by size:
3264 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6320 6320
Size: 6325
BlpI GC'TnA_GC
Cuts at: 2495 3208 3742 2495
Size: 713 534 5078
Fragments arranged by size:
5078 713 534
Bme1580I G_kGCm'C
Cuts at: 1790 2361 2973 3357 3398 4386 5542 1790
Size: 571 612 384 41 988 1156 2573
Fragments arranged by size:
2573 1156 988 612 571 384 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6325
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2618 4056 5014
Size: 416 53 83 399 24 1201 1438 958
Cuts at: 5014 442
Size: 1753
Fragments arranged by size:
1753 1438 1201 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 6325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2558 2563 2623 2839 3219 3461 3542
Size: 1106 213 5 60 216 380 242 81
Cuts at: 3542 4985 1239
Size: 1443 2579
Fragments arranged by size:
2579 1443 1106 380 242 216 213 81
60 5
Bpu10I CC'TnA_GC
Cuts at: 2564 2564
Size: 6325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4450 5315 5613 4450
Size: 865 298 5162
Fragments arranged by size:
5162 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2109 2435 3158 4988 882
Size: 617 610 326 723 1830 2219
Fragments arranged by size:
2219 1830 723 617 610 326
BsaAI yAC'GTr
Cuts at: 493 1204 3412 4116 493
Size: 711 2208 704 2702
Fragments arranged by size:
2702 2208 711 704
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2450 3192 3616 4132
Size: 53 83 186 936 917 742 424 516
Cuts at: 4132 4514 5149 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 917 742 635 516 424 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3498 4758 5156 5430 5577 5988 3498
Size: 1260 398 274 147 411 3835
Fragments arranged by size:
3835 1260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 3365 3395 1178
Size: 30 165 30 915 30 1017 30 4108
Fragments arranged by size:
4108 1017 915 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2536 2758 2761 3176 3386 1186
Size: 1350 222 3 415 210 4125
Fragments arranged by size:
4125 1350 415 222 210 3
BseYI C'CCAG_C
Cuts at: 2205 2950 3169 3425 5528 6024 2205
Size: 745 219 256 2103 496 2506
Fragments arranged by size:
2506 2103 745 496 256 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2125 2841 3027 3605 1163
Size: 573 389 716 186 578 3883
Fragments arranged by size:
3883 716 578 573 389 186
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 3775 4536 5564 1540
Size: 275 186 1498 276 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 276 275 186
BsiHKAI G_wGCw'C
Cuts at: 3111 4386 4471 5542 3111
Size: 1275 85 1071 3894
Fragments arranged by size:
3894 1275 1071 85
BsmI GAATG_Cn'
Cuts at: 3836 3929 3836
Size: 93 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2973 3111 3357 3398 3550 3644
Size: 399 571 612 138 246 41 152 94
Cuts at: 3644 4386 4471 5542 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 612 571 399 246 152
138 94 85 41
BspEI T'CCGG_A
Cuts at: 5988 5988
Size: 6325
BspHI T'CATG_A
Cuts at: 4214 5944 4214
Size: 1730 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 2147 2377 2618 2881 3469 3680 844
Size: 1259 44 230 241 263 588 211 3489
Fragments arranged by size:
3489 1259 588 263 241 230 211 44
BsrBI CCG'CTC
Cuts at: 1394 1823 4212 1394
Size: 429 2389 3507
Fragments arranged by size:
3507 2389 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2071 2220 4820 4994 65
Size: 1183 823 149 2600 174 1396
Fragments arranged by size:
2600 1396 1183 823 174 149
BsrFI r'CCGG_y
Cuts at: 1700 1810 3188 3609 4969 5156 1700
Size: 110 1378 421 1360 187 2869
Fragments arranged by size:
2869 1378 1360 421 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 4385 5397 4385
Size: 1012 5313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2061 2485 2801 794
Size: 1267 424 316 4318
Fragments arranged by size:
4318 1267 424 316
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6325
BstYI r'GATC_y
Cuts at: 1972 3316 3405 3655 4022 4407 4424 5876 5962
Size: 1344 89 250 367 385 17 1452 86
Cuts at: 5962 5974 6320 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1344 385 367 346 250 89
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2656 3361 513
Size: 552 1591 705 3477
Fragments arranged by size:
3477 1591 705 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5586
Fragments arranged by size:
5586 683 56
BtsI GCAGTG_nn'
Cuts at: 3015 3841 4635 4655 3015
Size: 826 794 20 4685
Fragments arranged by size:
4685 826 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6290
DraI TTT'AAA
Cuts at: 3629 3629
Size: 6325
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5332 809
Size: 507 4016 1802
Fragments arranged by size:
4016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3772 4661 6290
Size: 54 1425 50 275 18 1942 889 1629
Cuts at: 6290 8
Size: 43
Fragments arranged by size:
1942 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3772 1537
Size: 275 1960 4090
Fragments arranged by size:
4090 1960 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 4255 1353
Size: 830 2072 3423
Fragments arranged by size:
3423 2072 830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4912 5286 5432 387
Size: 1064 138 3323 374 146 1280
Fragments arranged by size:
3323 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2174 2345 2558 2563 2623 2839
Size: 393 405 137 171 213 5 60 216
Cuts at: 2839 3219 3461 3542 4388 4985 5772 6040 1239
Size: 380 242 81 846 597 787 268 1524
Fragments arranged by size:
1524 846 787 597 405 393 380 268
242 216 213 171 137 81 60 5
EcoO109I rG'GnC_Cy
Cuts at: 2357 2358 3241 3353 3354 3534 2357
Size: 1 883 112 1 180 5148
Fragments arranged by size:
5148 883 180 112 1 1
EcoRI G'AATT_C
Cuts at: 2470 3634 4012 2470
Size: 1164 378 4783
Fragments arranged by size:
4783 1164 378
FspI TGC'GCA
Cuts at: 4831 4831
Size: 6325
HaeII r_GCGC'y
Cuts at: 1678 2453 3195 5472 1678
Size: 775 742 2277 2531
Fragments arranged by size:
2531 2277 775 742
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2318 2350 3212 3244 3371
Size: 32 135 32 746 32 862 32 127
Cuts at: 3371 3403 4966 4998 5040 5072 1373
Size: 32 1563 32 42 32 2626
Fragments arranged by size:
2626 1563 862 746 135 127 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3669 3915 669
Size: 988 281 1731 246 3079
Fragments arranged by size:
3079 1731 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6325
HpaI GTT'AAC
Cuts at: 3915 3915
Size: 6325
KasI G'GCGC_C
Cuts at: 2449 3191 2449
Size: 742 5583
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3203 3231 5439 5623 1305
Size: 1898 28 2208 184 2007
Fragments arranged by size:
2208 2007 1898 184 28
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4503
Fragments arranged by size:
4503 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2909 3116 4283 4642 4801 518
Size: 648 1743 207 1167 359 159 2042
Fragments arranged by size:
2042 1743 1167 648 359 207 159
MspA1I CmG'CkG
Cuts at: 1606 2194 2400 2583 3741 4418 5566 5811 1606
Size: 588 206 183 1158 677 1148 245 2120
Fragments arranged by size:
2120 1158 1148 677 588 245 206 183
NaeI GCC'GGC
Cuts at: 3190 3190
Size: 6325
NarI GG'CG_CC
Cuts at: 2450 3192 2450
Size: 742 5583
NcoI C'CATG_G
Cuts at: 513 1065 3361 513
Size: 552 2296 3477
Fragments arranged by size:
3477 2296 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6325
NgoMIV G'CCGG_C
Cuts at: 3188 3188
Size: 6325
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 3683 5228 1132
Size: 97 2454 1545 2229
Fragments arranged by size:
2454 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2709 3452 1348
Size: 1361 743 4221
Fragments arranged by size:
4221 1361 743
PciI A'CATG_T
Cuts at: 5224 5224
Size: 6325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2528 2657 2692 3439 1108
Size: 1420 129 35 747 3994
Fragments arranged by size:
3994 1420 747 129 35
PfoI T'CCnGG_A
Cuts at: 2436 3438 3507 2436
Size: 1002 69 5254
Fragments arranged by size:
5254 1002 69
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PmlI CAC'GTG
Cuts at: 3412 3412
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 3895 3895
Size: 6325
PspOMI G'GGCC_C
Cuts at: 2357 3353 2357
Size: 996 5329
PstI C_TGCA'G
Cuts at: 830 2405 2490 2797 2806 3677 830
Size: 1575 85 307 9 871 3478
Fragments arranged by size:
3478 1575 871 307 85 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
PvuII CAG'CTG
Cuts at: 2400 2400
Size: 6325
RsrII CG'GwC_CG
Cuts at: 2305 2305
Size: 6325
SalI G'TCGA_C
Cuts at: 1936 3667 1936
Size: 1731 4594
SbfI CC_TGCA'GG
Cuts at: 3677 3677
Size: 6325
ScaI AGT'ACT
Cuts at: 2216 4573 6285 2216
Size: 2357 1712 2256
Fragments arranged by size:
2357 2256 1712
SfcI C'TryA_G
Cuts at: 826 1045 2401 2486 2793 2802 3673 4808 5489
Size: 219 1356 85 307 9 871 1135 681
Cuts at: 5489 5680 826
Size: 191 1471
Fragments arranged by size:
1471 1356 1135 871 681 307 219 191
85 9
SfoI GGC'GCC
Cuts at: 2451 3193 2451
Size: 742 5583
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 3188 1700
Size: 1488 4837
SmaI CCC'GGG
Cuts at: 1872 3652 1872
Size: 1780 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4429 5330 5592 820
Size: 197 926 2486 901 262 1553
Fragments arranged by size:
2486 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 3683 1132
Size: 2551 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6325
StyI C'CwwG_G
Cuts at: 513 1065 2513 3114 3244 3342 3361 3764 513
Size: 552 1448 601 130 98 19 403 3074
Fragments arranged by size:
3074 1448 601 552 403 130 98 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2197 4352 4369 4522 4707 5152 325
Size: 1255 617 2155 17 153 185 445 1498
Fragments arranged by size:
2155 1498 1255 617 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 4571 6283
Size: 274 80 33 51 1443 237 2357 1712
Cuts at: 6283 96
Size: 138
Fragments arranged by size:
2357 1712 1443 274 237 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6285 6317 6285
Size: 32 6293
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6325
XbaI T'CTAG_A
Cuts at: 3661 3661
Size: 6325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3340 3340
Size: 6325
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6325
XmaI C'CCGG_G
Cuts at: 1870 3650 1870
Size: 1780 4545
XmnI GAAnn'nnTTC
Cuts at: 4454 4454
Size: 6325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI