Restriction map for
pFN21AA1261
(Circular) (Six-base) MAPSORT of: pFN21AA1261.seq Check: 4042 from: 1 to: 7024
pFN21AA1261 7024 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2350 2350
Size: 7024
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4834 278
Size: 53 83 186 936 3298 2468
Fragments arranged by size:
3298 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4367 1937
Size: 2430 4594
Acc65I G'GTAC_C
Cuts at: 4345 4345
Size: 7024
AclI AA'CG_TT
Cuts at: 1563 5151 5524 1563
Size: 3588 373 3063
Fragments arranged by size:
3588 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 2445 2604 2727 5087 6471 6739 1632
Size: 539 274 159 123 2360 1384 268 1917
Fragments arranged by size:
2360 1917 1384 539 274 268 159 123
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6827
AflIII A'CryG_T
Cuts at: 1203 3505 3507 5923 6900 1203
Size: 2302 2 2416 977 1327
Fragments arranged by size:
2416 2302 1327 977 2
AgeI A'CCGG_T
Cuts at: 5855 5855
Size: 7024
AhdI GACnn_n'nnGTC
Cuts at: 5753 5753
Size: 7024
AleI CACnn'nnGTG
Cuts at: 1166 3294 1166
Size: 2128 4896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2079 1184
Size: 895 6129
AlwNI CAG_nnn'CTG
Cuts at: 3224 3939 4767 6339 3224
Size: 715 828 1572 3909
Fragments arranged by size:
3909 1572 828 715
ApaLI G'TGCA_C
Cuts at: 2289 5081 6237 2289
Size: 2792 1156 3076
Fragments arranged by size:
3076 2792 1156
ApoI r'AATT_y
Cuts at: 1493 2078 4333 4559 4711 6820 1493
Size: 585 2255 226 152 2109 1697
Fragments arranged by size:
2255 2109 1697 585 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5578 160
Size: 872 4546 1606
Fragments arranged by size:
4546 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
AvaI C'yCGr_G
Cuts at: 1870 1943 2802 4349 1870
Size: 73 859 1547 4545
Fragments arranged by size:
4545 1547 859 73
BamHI G'GATC_C
Cuts at: 2788 4354 4721 2788
Size: 1566 367 5091
Fragments arranged by size:
5091 1566 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 3896 4345 5800 618
Size: 325 217 627 263 1846 449 1455 1842
Fragments arranged by size:
1846 1842 1455 627 449 325 263 217
BanII G_rGCy'C
Cuts at: 1391 2469 2618 2813 3192 4343 1391
Size: 1078 149 195 379 1151 4072
Fragments arranged by size:
4072 1151 1078 379 195 149
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2189 928
Size: 971 290 5763
Fragments arranged by size:
5763 971 290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5215 5249 1903
Size: 34 3278 34 3678
Fragments arranged by size:
3678 3278 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2635 4916 6126 2635
Size: 2281 1210 3533
Fragments arranged by size:
3533 2281 1210
BclI T'GATC_A
Cuts at: 1548 2584 1548
Size: 1036 5988
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3558 5635 136
Size: 107 122 71 631 605 1886 2077 1525
Fragments arranged by size:
2077 1886 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3990 7019 3990
Size: 3029 3995
BlpI GC'TnA_GC
Cuts at: 4241 4441 4241
Size: 200 6824
Bme1580I G_kGCm'C
Cuts at: 1790 2293 3138 5085 6241 1790
Size: 503 845 1947 1156 2573
Fragments arranged by size:
2573 1947 1156 845 503
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7024
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4755 5713 442
Size: 416 53 83 399 24 3338 958 1753
Fragments arranged by size:
3338 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7024
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 2453 2506 4038 4207 5684 1239
Size: 1148 66 53 1532 169 1477 2579
Fragments arranged by size:
2579 1532 1477 1148 169 66 53
Bpu10I CC'TnA_GC
Cuts at: 2018 2018
Size: 7024
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 3061 4109 5149 6014 6312 2843
Size: 218 1048 1040 865 298 3555
Fragments arranged by size:
3555 1048 1040 865 298 218
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2438 5687 882
Size: 617 939 3249 2219
Fragments arranged by size:
3249 2219 939 617
BsaAI yAC'GTr
Cuts at: 493 1204 3508 4815 493
Size: 711 2304 1307 2702
Fragments arranged by size:
2702 2304 1307 711
BsaBI GATnn'nnATC
Cuts at: 7018 7018
Size: 7024
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3405 4831 5213 5848
Size: 53 83 186 936 1872 1426 382 635
Cuts at: 5848 275
Size: 1451
Fragments arranged by size:
1872 1451 1426 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5457 5855 6129 6276 6687 5457
Size: 398 274 147 411 5794
Fragments arranged by size:
5794 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2418 2448 2588 2618 2651
Size: 30 165 30 1015 30 140 30 33
Cuts at: 2651 2681 2968 2998 3185 3215 3458 3488 3968
Size: 30 287 30 187 30 243 30 480
Cuts at: 3968 3998 1178
Size: 30 4204
Fragments arranged by size:
4204 1015 480 287 243 187 165 140
33 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2671 2905 1186
Size: 1485 234 5305
Fragments arranged by size:
5305 1485 234
BseYI C'CCAG_C
Cuts at: 2409 2812 3185 3579 6227 6723 2409
Size: 403 373 394 2648 496 2710
Fragments arranged by size:
2710 2648 496 403 394 373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 3806 1163
Size: 573 621 1449 4381
Fragments arranged by size:
4381 1449 621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4474 5235 6263 1540
Size: 275 186 2473 761 1028 2301
Fragments arranged by size:
2473 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2293 2322 2530 2618 5085 5170 6241 2293
Size: 29 208 88 2467 85 1071 3076
Fragments arranged by size:
3076 2467 1071 208 88 85 29
BsmI GAATG_Cn'
Cuts at: 2284 3327 4186 4535 4628 2284
Size: 1043 859 349 93 4680
Fragments arranged by size:
4680 1043 859 349 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2968 2968
Size: 7024
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2293 2322 2469 2530 2618 2813 3138
Size: 399 503 29 147 61 88 195 325
Cuts at: 3138 3192 4343 5085 5170 6241 1391
Size: 54 1151 742 85 1071 2174
Fragments arranged by size:
2174 1151 1071 742 503 399 325 195
147 88 85 61 54 29
BspEI T'CCGG_A
Cuts at: 6687 6687
Size: 7024
BspHI T'CATG_A
Cuts at: 4102 4913 6643 4102
Size: 811 1730 4483
Fragments arranged by size:
4483 1730 811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2350 3954 4379 844
Size: 1506 1604 425 3489
Fragments arranged by size:
3489 1604 1506 425
BsrBI CCG'CTC
Cuts at: 1394 1823 3176 4911 1394
Size: 429 1353 1735 3507
Fragments arranged by size:
3507 1735 1353 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4057 5519 5693 65
Size: 1183 2809 1462 174 1396
Fragments arranged by size:
2809 1462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5668 5855 1700
Size: 110 3858 187 2869
Fragments arranged by size:
3858 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3510 96
Size: 3414 3610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7024
BssSI C'ACGA_G
Cuts at: 3499 5084 6096 3499
Size: 1585 1012 4427
Fragments arranged by size:
4427 1585 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3224 794
Size: 2430 4594
BstBI TT'CG_AA
Cuts at: 5828 5828
Size: 7024
BstEII G'GTnAC_C
Cuts at: 1184 2362 3481 1184
Size: 1178 1119 4727
Fragments arranged by size:
4727 1178 1119
BstXI CCAn_nnnn'nTGG
Cuts at: 3863 3863
Size: 7024
BstYI r'GATC_y
Cuts at: 1972 2788 3817 3990 4354 4721 5106 5123 6575
Size: 816 1029 173 364 367 385 17 1452
Cuts at: 6575 6661 6673 7019 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1029 816 385 367 364 346
173 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3083 3083
Size: 7024
BtgI C'CryG_G
Cuts at: 513 1065 2367 3460 4257 513
Size: 552 1302 1093 797 3280
Fragments arranged by size:
3280 1302 1093 797 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2754 3790 487
Size: 683 56 1528 1036 3721
Fragments arranged by size:
3721 1528 1036 683 56
BtsI GCAGTG_nn'
Cuts at: 2506 4038 4540 5334 5354 2506
Size: 1532 502 794 20 4176
Fragments arranged by size:
4176 1532 794 502 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6989
DraI TTT'AAA
Cuts at: 2077 4328 2077
Size: 2251 4773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3929 6031 809
Size: 507 2613 2102 1802
Fragments arranged by size:
2613 2102 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3753 4471 5360
Size: 54 1425 50 275 18 1923 718 889
Cuts at: 5360 6989 8
Size: 1629 43
Fragments arranged by size:
1923 1629 1425 889 718 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4471 1537
Size: 275 2659 4090
Fragments arranged by size:
4090 2659 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2623 4954 1353
Size: 756 514 2331 3423
Fragments arranged by size:
3423 2331 756 514
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5611 5985 6131 387
Size: 1064 138 4022 374 146 1280
Fragments arranged by size:
4022 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2616 2616
Size: 7024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2387 2445 2453 2506 2604 2727
Size: 393 539 216 58 8 53 98 123
Cuts at: 2727 4038 4207 5087 5684 6471 6739 1239
Size: 1311 169 880 597 787 268 1524
Fragments arranged by size:
1524 1311 880 787 597 539 393 268
216 169 123 98 58 53 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3838 3950 3838
Size: 112 6912
EcoO109I rG'GnC_Cy
Cuts at: 3087 3685 3936 3943 3087
Size: 598 251 7 6168
Fragments arranged by size:
6168 598 251 7
EcoRI G'AATT_C
Cuts at: 4333 4711 4333
Size: 378 6646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3796 3796
Size: 7024
FspI TGC'GCA
Cuts at: 5530 5530
Size: 7024
HaeII r_GCGC'y
Cuts at: 1678 6171 1678
Size: 4493 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2418 2450 2588 2592 2620
Size: 32 135 32 846 32 138 4 28
Cuts at: 2620 2624 2666 2698 2777 2809 3408 3440 3710
Size: 4 42 32 79 32 599 32 270
Cuts at: 3710 3742 3968 3982 4000 4014 5665 5697 5739
Size: 32 226 14 18 14 1651 32 42
Cuts at: 5739 5771 1373
Size: 32 2626
Fragments arranged by size:
2626 1651 846 599 270 226 138 135
79 42 42 32 32 32 32 32
32 32 32 32 28 18 14 14
4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2773 3064 4368 4614 669
Size: 988 281 835 291 1304 246 3079
Fragments arranged by size:
3079 1304 988 835 291 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7024
HpaI GTT'AAC
Cuts at: 4614 4614
Size: 7024
KpnI G_GTAC'C
Cuts at: 4349 4349
Size: 7024
MfeI C'AATT_G
Cuts at: 4623 4623
Size: 7024
MluI A'CGCG_T
Cuts at: 6900 6900
Size: 7024
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3140 3634 3722 6138 6322 1305
Size: 1835 494 88 2416 184 2007
Fragments arranged by size:
2416 2007 1835 494 184 88
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3755 10
Size: 54 1425 343 1923 3279
Fragments arranged by size:
3279 1923 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2427 2580 3294 3296 3809 3861 4101
Size: 648 1261 153 714 2 513 52 240
Cuts at: 4101 4256 4982 5341 5500 518
Size: 155 726 359 159 2042
Fragments arranged by size:
2042 1261 726 714 648 513 359 240
159 155 153 52 2
MspA1I CmG'CkG
Cuts at: 1606 2197 3221 3236 3873 3964 4440 5117 6265
Size: 591 1024 15 637 91 476 677 1148
Cuts at: 6265 6510 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1024 677 637 591 476 245
91 15
NcoI C'CATG_G
Cuts at: 513 1065 2367 513
Size: 552 1302 5170
Fragments arranged by size:
5170 1302 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7024
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7024
NotI GC'GGCC_GC
Cuts at: 4471 4471
Size: 7024
NruI TCG'CGA
Cuts at: 2013 2013
Size: 7024
NspI r_CATG'y
Cuts at: 1132 1229 3295 3437 4070 4382 5927 1132
Size: 97 2066 142 633 312 1545 2229
Fragments arranged by size:
2229 2066 1545 633 312 142 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7024
PciI A'CATG_T
Cuts at: 5923 5923
Size: 7024
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3835 3906 1108
Size: 2727 71 4226
Fragments arranged by size:
4226 2727 71
PfoI T'CCnGG_A
Cuts at: 3025 3331 3025
Size: 306 6718
PmeI GTTT'AAAC
Cuts at: 4328 4328
Size: 7024
PmlI CAC'GTG
Cuts at: 3508 3508
Size: 7024
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5093 5125 6606 6638 5093
Size: 32 1481 32 5479
Fragments arranged by size:
5479 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3087 3685 3936 3943 3087
Size: 598 251 7 6168
Fragments arranged by size:
6168 598 251 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7024
PsiI TTA'TAA
Cuts at: 4594 4594
Size: 7024
PspXI vC'TCGA_Gb
Cuts at: 2802 2802
Size: 7024
PstI C_TGCA'G
Cuts at: 830 3623 3794 3969 4376 830
Size: 2793 171 175 407 3478
Fragments arranged by size:
3478 2793 407 175 171
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7024
PvuII CAG'CTG
Cuts at: 3221 3873 3964 3221
Size: 652 91 6281
Fragments arranged by size:
6281 652 91
SacI G_AGCT'C
Cuts at: 2618 2618
Size: 7024
SalI G'TCGA_C
Cuts at: 1936 4366 1936
Size: 2430 4594
SapI GCTCTTCn'nnn_
Cuts at: 2623 2623
Size: 7024
SbfI CC_TGCA'GG
Cuts at: 4376 4376
Size: 7024
ScaI AGT'ACT
Cuts at: 2908 3670 5272 6984 2908
Size: 762 1602 1712 2948
Fragments arranged by size:
2948 1712 1602 762
SfcI C'TryA_G
Cuts at: 826 1045 3619 3790 3965 4372 5507 6188 6379
Size: 219 2574 171 175 407 1135 681 191
Cuts at: 6379 826
Size: 1471
Fragments arranged by size:
2574 1471 1135 681 407 219 191 175
171
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7024
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7024
SmaI CCC'GGG
Cuts at: 1872 4351 1872
Size: 2479 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2802 2858 3076 4124 5128 6029
Size: 197 926 859 56 218 1048 1004 901
Cuts at: 6029 6291 820
Size: 262 1553
Fragments arranged by size:
1553 1048 1004 926 901 859 262 218
197 56
SnaBI TAC'GTA
Cuts at: 493 4815 493
Size: 4322 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7024
SphI G_CATG'C
Cuts at: 1132 3437 4382 1132
Size: 2305 945 3774
Fragments arranged by size:
3774 2305 945
SspI AAT'ATT
Cuts at: 5 52 4948 5
Size: 47 4896 2081
Fragments arranged by size:
4896 2081 47
StuI AGG'CCT
Cuts at: 1517 3037 1517
Size: 1520 5504
StyI C'CwwG_G
Cuts at: 513 1065 2367 3771 3834 4463 513
Size: 552 1302 1404 63 629 3074
Fragments arranged by size:
3074 1404 1302 629 552 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2503 3570 5051 5068 5221 5406 5851
Size: 1255 923 1067 1481 17 153 185 445
Cuts at: 5851 325
Size: 1498
Fragments arranged by size:
1498 1481 1255 1067 923 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2906 3510 3668
Size: 274 80 33 51 1443 929 604 158
Cuts at: 3668 5270 6982 96
Size: 1602 1712 138
Fragments arranged by size:
1712 1602 1443 929 604 274 158 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2651 2683 3185 3217 3458 3490 6984 7016 2651
Size: 32 502 32 241 32 3494 32 2659
Fragments arranged by size:
3494 2659 502 241 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2430 1316
Size: 1114 5910
XbaI T'CTAG_A
Cuts at: 4360 4360
Size: 7024
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3685 4020 3685
Size: 335 6689
XhoI C'TCGA_G
Cuts at: 1943 2802 1943
Size: 859 6165
XmaI C'CCGG_G
Cuts at: 1870 4349 1870
Size: 2479 4545
XmnI GAAnn'nnTTC
Cuts at: 2118 5153 2118
Size: 3035 3989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4832 276
Size: 53 83 186 936 3298 2468
Fragments arranged by size:
3298 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PspOMI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI