Restriction map for
pFN21AA1256
(Circular) (Six-base) MAPSORT of: pFN21AA1256.seq Check: 2162 from: 1 to: 9715
pFN21AA1256 9715 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5302 5302
Size: 9715
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7525 278
Size: 53 83 186 936 5989 2468
Fragments arranged by size:
5989 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2617 6486 7058 1937
Size: 680 3869 572 4594
Fragments arranged by size:
4594 3869 680 572
Acc65I G'GTAC_C
Cuts at: 3667 7036 3667
Size: 3369 6346
AclI AA'CG_TT
Cuts at: 1563 7842 8215 1563
Size: 6279 373 3063
Fragments arranged by size:
6279 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2085 2094 2288 2421 2807 3515 3620 5091
Size: 453 9 194 133 386 708 105 1471
Cuts at: 5091 5591 5682 5747 6603 7778 9162 9430 1632
Size: 500 91 65 856 1175 1384 268 1917
Fragments arranged by size:
1917 1471 1384 1175 856 708 500 453
386 268 194 133 105 91 65 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9518
AflIII A'CryG_T
Cuts at: 1203 2046 8614 9591 1203
Size: 843 6568 977 1327
Fragments arranged by size:
6568 1327 977 843
AgeI A'CCGG_T
Cuts at: 8546 8546
Size: 9715
AhdI GACnn_n'nnGTC
Cuts at: 8444 8444
Size: 9715
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9715
AlwNI CAG_nnn'CTG
Cuts at: 2635 4493 5835 7458 9030 2635
Size: 1858 1342 1623 1572 3320
Fragments arranged by size:
3320 1858 1623 1572 1342
ApaI G_GGCC'C
Cuts at: 6219 6219
Size: 9715
ApaLI G'TGCA_C
Cuts at: 7772 8928 7772
Size: 1156 8559
ApoI r'AATT_y
Cuts at: 1493 2756 3396 4104 4333 5004 5903 6772 6879
Size: 1263 640 708 229 671 899 869 107
Cuts at: 6879 6894 7024 7250 7402 9511 1493
Size: 15 130 226 152 2109 1697
Fragments arranged by size:
2109 1697 1263 899 869 708 671 640
229 226 152 130 107 15
AseI AT'TA_AT
Cuts at: 160 1032 2339 3024 5165 5385 6510 8269 160
Size: 872 1307 685 2141 220 1125 1759 1606
Fragments arranged by size:
2141 1759 1606 1307 1125 872 685 220
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9715
AvaI C'yCGr_G
Cuts at: 1870 1943 6068 7040 1870
Size: 73 4125 972 4545
Fragments arranged by size:
4545 4125 972 73
BamHI G'GATC_C
Cuts at: 6144 7045 7412 6144
Size: 901 367 8447
Fragments arranged by size:
8447 901 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3667 6630 7036 8491 618
Size: 325 217 627 1880 2963 406 1455 1842
Fragments arranged by size:
2963 1880 1842 1455 627 406 325 217
BanII G_rGCy'C
Cuts at: 1391 5361 5703 6219 6722 7034 1391
Size: 3970 342 516 503 312 4072
Fragments arranged by size:
4072 3970 516 503 342 312
BbeI G_GCGC'C
Cuts at: 6634 6634
Size: 9715
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2418 5756 5882 5892 6451 928
Size: 971 519 3338 126 10 559 4192
Fragments arranged by size:
4192 3338 971 559 519 126 10
BbvCI CC'TCA_GC
Cuts at: 2388 2727 4128 4167 2388
Size: 339 1401 39 7936
Fragments arranged by size:
7936 1401 339 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7906 7940 1903
Size: 34 5969 34 3678
Fragments arranged by size:
5969 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4147 7607 8817 4147
Size: 3460 1210 5045
Fragments arranged by size:
5045 3460 1210
BclI T'GATC_A
Cuts at: 1548 2131 1548
Size: 583 9132
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 8326 136
Size: 107 122 71 631 605 337 6317 1525
Fragments arranged by size:
6317 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3516 9710 3516
Size: 6194 3521
BlpI GC'TnA_GC
Cuts at: 5802 7132 5802
Size: 1330 8385
Bme1580I G_kGCm'C
Cuts at: 1790 6219 7776 8932 1790
Size: 4429 1557 1156 2573
Fragments arranged by size:
4429 2573 1557 1156
BmgBI CAC'GTC
Cuts at: 1318 6814 1318
Size: 5496 4219
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2712 7446 8404
Size: 416 53 83 399 24 1295 4734 958
Cuts at: 8404 442
Size: 1753
Fragments arranged by size:
4734 1753 1295 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 5061 1056
Size: 4005 5710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6106 6106
Size: 9715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2385 4064 4284 4935 5088 5938 6038 6158
Size: 1146 1679 220 651 153 850 100 120
Cuts at: 6158 8375 1239
Size: 2217 2579
Fragments arranged by size:
2579 2217 1679 1146 850 651 220 153
120 100
Bpu10I CC'TnA_GC
Cuts at: 2388 2727 2976 4128 4167 5097 2388
Size: 339 249 1152 39 930 7006
Fragments arranged by size:
7006 1152 930 339 249 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3503 6078 7840 8705 9003 3503
Size: 2575 1762 865 298 4215
Fragments arranged by size:
4215 2575 1762 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5450 6834 8378 882
Size: 617 3951 1384 1544 2219
Fragments arranged by size:
3951 2219 1544 1384 617
BsaAI yAC'GTr
Cuts at: 493 1204 2141 6157 7506 493
Size: 711 937 4016 1349 2702
Fragments arranged by size:
4016 2702 1349 937 711
BsaBI GATnn'nnATC
Cuts at: 4585 9709 4585
Size: 5124 4591
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3148 6548 6631 7522
Size: 53 83 186 936 1615 3400 83 891
Cuts at: 7522 7904 8539 275
Size: 382 635 1451
Fragments arranged by size:
3400 1615 1451 936 891 635 382 186
83 83 53
BsaWI w'CCGG_w
Cuts at: 8148 8546 8820 8967 9378 8148
Size: 398 274 147 411 8485
Fragments arranged by size:
8485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2956 2986 6035 6065 1178
Size: 30 165 30 1553 30 3049 30 4828
Fragments arranged by size:
4828 3049 1553 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3015 3077 4145 4184 6095 1186
Size: 1829 62 1068 39 1911 4806
Fragments arranged by size:
4806 1911 1829 1068 62 39
BseYI C'CCAG_C
Cuts at: 8918 9414 8918
Size: 496 9219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2391 5795 6179 6257 6551 1163
Size: 573 655 3404 384 78 294 4327
Fragments arranged by size:
4327 3404 655 573 384 294 78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7165 7926 8954 1540
Size: 275 186 5164 761 1028 2301
Fragments arranged by size:
5164 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5361 5703 6092 7776 7861 8932 5361
Size: 342 389 1684 85 1071 6144
Fragments arranged by size:
6144 1684 1071 389 342 85
BsmI GAATG_Cn'
Cuts at: 5949 7226 7319 5949
Size: 1277 93 8345
Fragments arranged by size:
8345 1277 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5361 5703 6092 6219 6722 7034 7776
Size: 399 3571 342 389 127 503 312 742
Cuts at: 7776 7861 8932 1391
Size: 85 1071 2174
Fragments arranged by size:
3571 2174 1071 742 503 399 389 342
312 127 85
BspEI T'CCGG_A
Cuts at: 9378 9378
Size: 9715
BspHI T'CATG_A
Cuts at: 5566 7604 9334 5566
Size: 2038 1730 5947
Fragments arranged by size:
5947 2038 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5302 6993 7070 844
Size: 4458 1691 77 3489
Fragments arranged by size:
4458 3489 1691 77
BsrBI CCG'CTC
Cuts at: 1394 1823 5590 6073 6606 7602 1394
Size: 429 3767 483 533 996 3507
Fragments arranged by size:
3767 3507 996 533 483 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2393 2561 2925 8210 8384 65
Size: 1183 1145 168 364 5285 174 1396
Fragments arranged by size:
5285 1396 1183 1145 364 174 168
BsrFI r'CCGG_y
Cuts at: 1700 1810 2167 5770 8359 8546 1700
Size: 110 357 3603 2589 187 2869
Fragments arranged by size:
3603 2869 2589 357 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9715
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9715
BssSI C'ACGA_G
Cuts at: 7775 8787 7775
Size: 1012 8703
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5117 794
Size: 4323 5392
BstBI TT'CG_AA
Cuts at: 6898 8519 6898
Size: 1621 8094
BstEII G'GTnAC_C
Cuts at: 1184 4951 1184
Size: 3767 5948
BstXI CCAn_nnnn'nTGG
Cuts at: 2049 4964 5852 6211 2049
Size: 2915 888 359 5553
Fragments arranged by size:
5553 2915 888 359
BstYI r'GATC_y
Cuts at: 1972 2648 3516 4580 5485 6144 6799 7045 7412
Size: 676 868 1064 905 659 655 246 367
Cuts at: 7412 7797 7814 9266 9352 9364 9710 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1064 905 868 676 659 655
385 367 346 246 86 17 12
BstZ17I GTA'TAC
Cuts at: 2618 2618
Size: 9715
BtgI C'CryG_G
Cuts at: 513 1065 5930 513
Size: 552 4865 4298
Fragments arranged by size:
4865 4298 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6462 487
Size: 683 56 5236 3740
Fragments arranged by size:
5236 3740 683 56
BtsI GCAGTG_nn'
Cuts at: 3596 5996 6167 7231 8025 8045 3596
Size: 2400 171 1064 794 20 5266
Fragments arranged by size:
5266 2400 1064 794 171 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9715
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9680
DraI TTT'AAA
Cuts at: 5910 7019 5910
Size: 1109 8606
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8722 809
Size: 507 7406 1802
Fragments arranged by size:
7406 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2937 5796 7162
Size: 54 1425 50 275 18 1107 2859 1366
Cuts at: 7162 8051 9680 8
Size: 889 1629 43
Fragments arranged by size:
2859 1629 1425 1366 1107 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7162 1537
Size: 275 5350 4090
Fragments arranged by size:
5350 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2898 3054 3204 4107 4744 5580 6290 7645
Size: 1545 156 150 903 637 836 710 1355
Cuts at: 7645 1353
Size: 3423
Fragments arranged by size:
3423 1545 1355 903 836 710 637 156
150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8302 8676 8822 387
Size: 1064 138 6713 374 146 1280
Fragments arranged by size:
6713 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5359 5701 5359
Size: 342 9373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2094 2288 2385 2421 2807 3515
Size: 393 453 9 194 97 36 386 708
Cuts at: 3515 3620 4064 4284 4935 5088 5091 5591 5682
Size: 105 444 220 651 153 3 500 91
Cuts at: 5682 5747 5938 6038 6158 6603 7778 8375 9162
Size: 65 191 100 120 445 1175 597 787
Cuts at: 9162 9430 1239
Size: 268 1524
Fragments arranged by size:
1524 1175 787 708 651 597 500 453
445 444 393 386 268 220 194 191
153 120 105 100 97 91 65 36
9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2112 2210 3729 2112
Size: 98 1519 8098
Fragments arranged by size:
8098 1519 98
EcoO109I rG'GnC_Cy
Cuts at: 2706 4636 6215 6216 6920 6952 2706
Size: 1930 1579 1 704 32 5469
Fragments arranged by size:
5469 1930 1579 704 32 1
EcoRI G'AATT_C
Cuts at: 3396 7024 7402 3396
Size: 3628 378 5709
Fragments arranged by size:
5709 3628 378
EcoRV GAT'ATC
Cuts at: 2789 2789
Size: 9715
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4061 4429 4061
Size: 368 9347
FspI TGC'GCA
Cuts at: 8221 8221
Size: 9715
HaeII r_GCGC'y
Cuts at: 1678 6634 8862 1678
Size: 4956 2228 2531
Fragments arranged by size:
4956 2531 2228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2455 2487 2633 2665 2773
Size: 32 135 32 883 32 146 32 108
Cuts at: 2773 2805 2956 2988 4483 4515 6035 6067 8356
Size: 32 151 32 1495 32 1520 32 2289
Cuts at: 8356 8388 8430 8462 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2289 1520 1495 883 151 146 135
108 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5495 5689 7059 7305 669
Size: 988 281 3557 194 1370 246 3079
Fragments arranged by size:
3557 3079 1370 988 281 246 194
HindIII A'AGCT_T
Cuts at: 748 2270 3653 4818 6591 748
Size: 1522 1383 1165 1773 3872
Fragments arranged by size:
3872 1773 1522 1383 1165
HpaI GTT'AAC
Cuts at: 5689 7305 5689
Size: 1616 8099
KasI G'GCGC_C
Cuts at: 6630 6630
Size: 9715
KpnI G_GTAC'C
Cuts at: 3671 7040 3671
Size: 3369 6346
MfeI C'AATT_G
Cuts at: 5538 7314 5538
Size: 1776 7939
MluI A'CGCG_T
Cuts at: 9591 9591
Size: 9715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3468 3565 4849 5736 8829 9013 1305
Size: 2163 97 1284 887 3093 184 2007
Fragments arranged by size:
3093 2163 2007 1284 887 184 97
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2939 10
Size: 54 1425 343 1107 6786
Fragments arranged by size:
6786 1425 1107 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2249 7673 8032 8191 518
Size: 648 861 222 5424 359 159 2042
Fragments arranged by size:
5424 2042 861 648 359 222 159
MspA1I CmG'CkG
Cuts at: 1606 2405 2848 4832 5801 5841 5932 7131 7808
Size: 799 443 1984 969 40 91 1199 677
Cuts at: 7808 8956 9201 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1984 1199 1148 969 799 677 443
245 91 40
NaeI GCC'GGC
Cuts at: 2169 2169
Size: 9715
NarI GG'CG_CC
Cuts at: 6631 6631
Size: 9715
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 9163
NdeI CA'TA_TG
Cuts at: 387 5113 387
Size: 4726 4989
NgoMIV G'CCGG_C
Cuts at: 2167 2167
Size: 9715
NheI G'CTAG_C
Cuts at: 1052 5057 1052
Size: 4005 5710
NotI GC'GGCC_GC
Cuts at: 7162 7162
Size: 9715
NspI r_CATG'y
Cuts at: 1132 1229 2050 2371 3709 5818 6647 7073 8618
Size: 97 821 321 1338 2109 829 426 1545
Cuts at: 8618 1132
Size: 2229
Fragments arranged by size:
2229 2109 1545 1338 829 821 426 321
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9715
PciI A'CATG_T
Cuts at: 2046 8614 2046
Size: 6568 3147
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9715
PfoI T'CCnGG_A
Cuts at: 4080 4646 6747 4080
Size: 566 2101 7048
Fragments arranged by size:
7048 2101 566
PmeI GTTT'AAAC
Cuts at: 7019 7019
Size: 9715
PmlI CAC'GTG
Cuts at: 6157 6157
Size: 9715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7784 7816 9297 9329 7784
Size: 32 1481 32 8170
Fragments arranged by size:
8170 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2706 6952 2706
Size: 4246 5469
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9715
PsiI TTA'TAA
Cuts at: 3918 7285 3918
Size: 3367 6348
PspOMI G'GGCC_C
Cuts at: 6215 6215
Size: 9715
PspXI vC'TCGA_Gb
Cuts at: 6068 6068
Size: 9715
PstI C_TGCA'G
Cuts at: 830 5840 6990 7067 830
Size: 5010 1150 77 3478
Fragments arranged by size:
5010 3478 1150 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9715
PvuII CAG'CTG
Cuts at: 2405 2848 4832 5841 2405
Size: 443 1984 1009 6279
Fragments arranged by size:
6279 1984 1009 443
RsrII CG'GwC_CG
Cuts at: 6130 6130
Size: 9715
SacI G_AGCT'C
Cuts at: 5361 5703 5361
Size: 342 9373
SacII CC_GC'GG
Cuts at: 5933 5933
Size: 9715
SalI G'TCGA_C
Cuts at: 1936 7057 1936
Size: 5121 4594
SapI GCTCTTCn'nnn_
Cuts at: 5580 5580
Size: 9715
SbfI CC_TGCA'GG
Cuts at: 7067 7067
Size: 9715
ScaI AGT'ACT
Cuts at: 7963 9675 7963
Size: 1712 8003
SexAI A'CCwGG_T
Cuts at: 4303 4303
Size: 9715
SfcI C'TryA_G
Cuts at: 826 1045 4425 5836 6657 6986 7063 8198 8879
Size: 219 3380 1411 821 329 77 1135 681
Cuts at: 8879 9070 826
Size: 191 1471
Fragments arranged by size:
3380 1471 1411 1135 821 681 329 219
191 77
SfoI GGC'GCC
Cuts at: 6632 6632
Size: 9715
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9715
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9715
SmaI CCC'GGG
Cuts at: 1872 7042 1872
Size: 5170 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3482 6068 6093 7819 8720 8982
Size: 197 926 1539 2586 25 1726 901 262
Cuts at: 8982 820
Size: 1553
Fragments arranged by size:
2586 1726 1553 1539 926 901 262 197
25
SnaBI TAC'GTA
Cuts at: 493 7506 493
Size: 7013 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9715
SphI G_CATG'C
Cuts at: 1132 2371 7073 1132
Size: 1239 4702 3774
Fragments arranged by size:
4702 3774 1239
SspI AAT'ATT
Cuts at: 5 52 4947 5163 7639 5
Size: 47 4895 216 2476 2081
Fragments arranged by size:
4895 2476 2081 216 47
StuI AGG'CCT
Cuts at: 1517 4412 6171 1517
Size: 2895 1759 5061
Fragments arranged by size:
5061 2895 1759
StyI C'CwwG_G
Cuts at: 513 1065 5370 7154 513
Size: 552 4305 1784 3074
Fragments arranged by size:
4305 3074 1784 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2710 5178 5597 6147 6517 7742 7759
Size: 1255 1130 2468 419 550 370 1225 17
Cuts at: 7759 7912 8097 8542 325
Size: 153 185 445 1498
Fragments arranged by size:
2468 1498 1255 1225 1130 550 445 419
370 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6558 7961 9673
Size: 274 80 33 51 1443 4581 1403 1712
Cuts at: 9673 96
Size: 138
Fragments arranged by size:
4581 1712 1443 1403 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3575 3607 6745 6777 9675 9707 3575
Size: 32 3138 32 2898 32 3583
Fragments arranged by size:
3583 3138 2898 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3375 1316
Size: 2059 7656
XbaI T'CTAG_A
Cuts at: 7051 7051
Size: 9715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5496 5496
Size: 9715
XhoI C'TCGA_G
Cuts at: 1943 6068 1943
Size: 4125 5590
XmaI C'CCGG_G
Cuts at: 1870 7040 1870
Size: 5170 4545
XmnI GAAnn'nnTTC
Cuts at: 4104 6353 7844 4104
Size: 2249 1491 5975
Fragments arranged by size:
5975 2249 1491
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7523 276
Size: 53 83 186 936 5989 2468
Fragments arranged by size:
5989 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
Bsu36I DraIII FseI FspAI NruI NsiI PacI PsrI
SanDI SfiI SrfI SwaI