Restriction map for
pFN21AA1254
(Circular) (Six-base) MAPSORT of: pFN21AA1254.seq Check: 3288 from: 1 to: 6976
pFN21AA1254 6976 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4786 278
Size: 53 83 186 936 3250 2468
Fragments arranged by size:
3250 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2435 4319 1937
Size: 498 1884 4594
Fragments arranged by size:
4594 1884 498
Acc65I G'GTAC_C
Cuts at: 4297 4297
Size: 6976
AclI AA'CG_TT
Cuts at: 1563 5103 5476 1563
Size: 3540 373 3063
Fragments arranged by size:
3540 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 2595 2892 5039 6423 6691 1632
Size: 918 45 297 2147 1384 268 1917
Fragments arranged by size:
2147 1917 1384 918 297 268 45
AflII C'TTAA_G
Cuts at: 820 1017 3065 820
Size: 197 2048 4731
Fragments arranged by size:
4731 2048 197
AflIII A'CryG_T
Cuts at: 1203 3993 5875 6852 1203
Size: 2790 1882 977 1327
Fragments arranged by size:
2790 1882 1327 977
AgeI A'CCGG_T
Cuts at: 5807 5807
Size: 6976
AhdI GACnn_n'nnGTC
Cuts at: 5705 5705
Size: 6976
AleI CACnn'nnGTG
Cuts at: 1166 3944 1166
Size: 2778 4198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6976
AlwNI CAG_nnn'CTG
Cuts at: 2456 2573 4719 6291 2456
Size: 117 2146 1572 3141
Fragments arranged by size:
3141 2146 1572 117
ApaLI G'TGCA_C
Cuts at: 5033 6189 5033
Size: 1156 5820
ApoI r'AATT_y
Cuts at: 1493 2014 2086 3496 3575 3809 3826 3865 3968
Size: 521 72 1410 79 234 17 39 103
Cuts at: 3968 4285 4511 4663 6772 1493
Size: 317 226 152 2109 1697
Fragments arranged by size:
2109 1697 1410 521 317 234 226 152
103 79 72 39 17
AseI AT'TA_AT
Cuts at: 160 1032 5530 160
Size: 872 4498 1606
Fragments arranged by size:
4498 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6976
AvaI C'yCGr_G
Cuts at: 1870 1943 4301 1870
Size: 73 2358 4545
Fragments arranged by size:
4545 2358 73
BamHI G'GATC_C
Cuts at: 4306 4673 4306
Size: 367 6609
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3730 4297 5752 618
Size: 325 217 627 1943 567 1455 1842
Fragments arranged by size:
1943 1842 1455 627 567 325 217
BanII G_rGCy'C
Cuts at: 1391 2118 2417 4295 1391
Size: 727 299 1878 4072
Fragments arranged by size:
4072 1878 727 299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2286 2406 2558 2583 928
Size: 971 387 120 152 25 5321
Fragments arranged by size:
5321 971 387 152 120 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5167 5201 1903
Size: 34 3230 34 3678
Fragments arranged by size:
3678 3230 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2950 4003 4868 6078 2950
Size: 1053 865 1210 3848
Fragments arranged by size:
3848 1210 1053 865
BclI T'GATC_A
Cuts at: 1548 4036 4081 1548
Size: 2488 45 4443
Fragments arranged by size:
4443 2488 45
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2193 5587 136
Size: 107 122 71 631 605 521 3394 1525
Fragments arranged by size:
3394 1525 631 605 521 122 107 71
BglII A'GATC_T
Cuts at: 6971 6971
Size: 6976
BlpI GC'TnA_GC
Cuts at: 4393 4393
Size: 6976
Bme1580I G_kGCm'C
Cuts at: 1790 5037 6193 1790
Size: 3247 1156 2573
Fragments arranged by size:
3247 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6976
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2511 2788 4707
Size: 416 53 83 399 24 1094 277 1919
Cuts at: 4707 5665 442
Size: 958 1753
Fragments arranged by size:
1919 1753 1094 958 416 399 277 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2966 3182 3188 5636 1239
Size: 1727 216 6 2448 2579
Fragments arranged by size:
2579 2448 1727 216 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 5101 5966 6264 2351
Size: 2750 865 298 3063
Fragments arranged by size:
3063 2750 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2651 5639 882
Size: 617 1152 2988 2219
Fragments arranged by size:
2988 2219 1152 617
BsaAI yAC'GTr
Cuts at: 493 1204 4767 493
Size: 711 3563 2702
Fragments arranged by size:
3563 2702 711
BsaBI GATnn'nnATC
Cuts at: 2681 6970 2681
Size: 4289 2687
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2536 4783 5165 5800
Size: 53 83 186 936 1003 2247 382 635
Cuts at: 5800 275
Size: 1451
Fragments arranged by size:
2247 1451 1003 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5409 5807 6081 6228 6639 5409
Size: 398 274 147 411 5746
Fragments arranged by size:
5746 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3113 3143 1178
Size: 30 165 30 1710 30 5011
Fragments arranged by size:
5011 1710 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 1186
Size: 976 6000
BseYI C'CCAG_C
Cuts at: 6179 6675 6179
Size: 496 6480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6403
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4426 5187 6215 1540
Size: 275 186 2425 761 1028 2301
Fragments arranged by size:
2425 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3913 5037 5122 6193 3913
Size: 1124 85 1071 4696
Fragments arranged by size:
4696 1124 1071 85
BsmI GAATG_Cn'
Cuts at: 4487 4580 4487
Size: 93 6883
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2417 3913 4295 5037 5122 6193
Size: 399 328 299 1496 382 742 85 1071
Cuts at: 6193 1391
Size: 2174
Fragments arranged by size:
2174 1496 1071 742 399 382 328 299
85
BspEI T'CCGG_A
Cuts at: 6639 6639
Size: 6976
BspHI T'CATG_A
Cuts at: 2059 4865 6595 2059
Size: 2806 1730 2440
Fragments arranged by size:
2806 2440 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4331 844
Size: 3487 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4863 1394
Size: 429 3040 3507
Fragments arranged by size:
3507 3040 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2159 5471 5645 65
Size: 1183 911 3312 174 1396
Fragments arranged by size:
3312 1396 1183 911 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2580 5620 5807 1700
Size: 110 770 3040 187 2869
Fragments arranged by size:
3040 2869 770 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6976
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6976
BssSI C'ACGA_G
Cuts at: 5036 6048 5036
Size: 1012 5964
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6976
BstBI TT'CG_AA
Cuts at: 3324 5780 3324
Size: 2456 4520
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6976
BstXI CCAn_nnnn'nTGG
Cuts at: 2708 2708
Size: 6976
BstYI r'GATC_y
Cuts at: 1972 4306 4673 5058 5075 6527 6613 6625 6971
Size: 2334 367 385 17 1452 86 12 346
Cuts at: 6971 1972
Size: 1977
Fragments arranged by size:
2334 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2436 2436
Size: 6976
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6237
Fragments arranged by size:
6237 683 56
BtsI GCAGTG_nn'
Cuts at: 4492 5286 5306 4492
Size: 794 20 6162
Fragments arranged by size:
6162 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6976
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6941
DraI TTT'AAA
Cuts at: 3613 3805 4280 3613
Size: 192 475 6309
Fragments arranged by size:
6309 475 192
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5983 809
Size: 507 4667 1802
Fragments arranged by size:
4667 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2698 4423 5312
Size: 54 1425 50 275 18 868 1725 889
Cuts at: 5312 6941 8
Size: 1629 43
Fragments arranged by size:
1725 1629 1425 889 868 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4423 1537
Size: 275 2611 4090
Fragments arranged by size:
4090 2611 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2422 2976 3075 4097 4906 1353
Size: 1069 554 99 1022 809 3423
Fragments arranged by size:
3423 1069 1022 809 554 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5563 5937 6083 387
Size: 1064 138 3974 374 146 1280
Fragments arranged by size:
3974 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2550 2595 2892 2966 3182 3188 5039
Size: 393 918 45 297 74 216 6 1851
Cuts at: 5039 5636 6423 6691 1239
Size: 597 787 268 1524
Fragments arranged by size:
1851 1524 918 787 597 393 297 268
216 74 45 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2948 2948
Size: 6976
EcoO109I rG'GnC_Cy
Cuts at: 3758 3758
Size: 6976
EcoRI G'AATT_C
Cuts at: 2086 3496 3809 4285 4663 2086
Size: 1410 313 476 378 4399
Fragments arranged by size:
4399 1410 476 378 313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2168 2864 2905 3337 3649 4142 2168
Size: 696 41 432 312 493 5002
Fragments arranged by size:
5002 696 493 432 312 41
FspI TGC'GCA
Cuts at: 5482 5482
Size: 6976
HaeII r_GCGC'y
Cuts at: 1678 6123 1678
Size: 4445 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 2614 2646 2816
Size: 32 135 32 899 32 111 32 170
Cuts at: 2816 2848 2870 2902 5617 5649 5691 5723 1373
Size: 32 22 32 2715 32 42 32 2626
Fragments arranged by size:
2715 2626 899 170 135 111 42 32
32 32 32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 2735 2801 3132 4320 4566 669
Size: 988 281 797 66 331 1188 246 3079
Fragments arranged by size:
3079 1188 988 797 331 281 246 66
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6976
HpaI GTT'AAC
Cuts at: 4566 4566
Size: 6976
KpnI G_GTAC'C
Cuts at: 4301 4301
Size: 6976
MfeI C'AATT_G
Cuts at: 2250 4575 2250
Size: 2325 4651
MluI A'CGCG_T
Cuts at: 6852 6852
Size: 6976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2024 2840 4158 6090 6274 1305
Size: 719 816 1318 1932 184 2007
Fragments arranged by size:
2007 1932 1318 816 719 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2700 10
Size: 54 1425 343 868 4286
Fragments arranged by size:
4286 1425 868 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2706 2760 3944 4934 5293 5452 518
Size: 648 1540 54 1184 990 359 159 2042
Fragments arranged by size:
2042 1540 1184 990 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2109 3680 4392 5069 6217 6462 1606
Size: 503 1571 712 677 1148 245 2120
Fragments arranged by size:
2120 1571 1148 712 677 503 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6424
NdeI CA'TA_TG
Cuts at: 387 4228 387
Size: 3841 3135
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6976
NotI GC'GGCC_GC
Cuts at: 4423 4423
Size: 6976
NspI r_CATG'y
Cuts at: 1132 1229 3997 4334 5879 1132
Size: 97 2768 337 1545 2229
Fragments arranged by size:
2768 2229 1545 337 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6976
PciI A'CATG_T
Cuts at: 3993 5875 3993
Size: 1882 5094
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6976
PmeI GTTT'AAAC
Cuts at: 4280 4280
Size: 6976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5045 5077 6558 6590 5045
Size: 32 1481 32 5431
Fragments arranged by size:
5431 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4237 1613
Size: 2624 4352
PsiI TTA'TAA
Cuts at: 2829 4546 2829
Size: 1717 5259
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2256 2288 2256
Size: 32 6944
PstI C_TGCA'G
Cuts at: 830 4328 830
Size: 3498 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6976
PvuII CAG'CTG
Cuts at: 2109 3680 2109
Size: 1571 5405
SalI G'TCGA_C
Cuts at: 1936 4318 1936
Size: 2382 4594
SapI GCTCTTCn'nnn_
Cuts at: 2422 4097 2422
Size: 1675 5301
SbfI CC_TGCA'GG
Cuts at: 4328 4328
Size: 6976
ScaI AGT'ACT
Cuts at: 3037 5224 6936 3037
Size: 2187 1712 3077
Fragments arranged by size:
3077 2187 1712
SfcI C'TryA_G
Cuts at: 826 1045 2916 3735 4324 5459 6140 6331 826
Size: 219 1871 819 589 1135 681 191 1471
Fragments arranged by size:
1871 1471 1135 819 681 589 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6976
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6976
SmaI CCC'GGG
Cuts at: 1872 4303 1872
Size: 2431 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2330 3065 5080 5981 6243 820
Size: 197 926 387 735 2015 901 262 1553
Fragments arranged by size:
2015 1553 926 901 735 387 262 197
SnaBI TAC'GTA
Cuts at: 493 4767 493
Size: 4274 2702
SpeI A'CTAG_T
Cuts at: 152 3175 152
Size: 3023 3953
SphI G_CATG'C
Cuts at: 1132 4334 1132
Size: 3202 3774
SspI AAT'ATT
Cuts at: 5 52 3015 4900 5
Size: 47 2963 1885 2081
Fragments arranged by size:
2963 2081 1885 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6976
StyI C'CwwG_G
Cuts at: 513 1065 3504 4415 513
Size: 552 2439 911 3074
Fragments arranged by size:
3074 2439 911 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5003 5020 5173 5358 5803 325
Size: 1255 3423 17 153 185 445 1498
Fragments arranged by size:
3423 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3035 5222 6934
Size: 274 80 33 51 1443 1058 2187 1712
Cuts at: 6934 96
Size: 138
Fragments arranged by size:
2187 1712 1443 1058 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4202 4234 6936 6968 4202
Size: 32 2702 32 4210
Fragments arranged by size:
4210 2702 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6976
XbaI T'CTAG_A
Cuts at: 4312 4312
Size: 6976
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3212 3212
Size: 6976
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6976
XmaI C'CCGG_G
Cuts at: 1870 4301 1870
Size: 2431 4545
XmnI GAAnn'nnTTC
Cuts at: 3575 4091 5105 3575
Size: 516 1014 5446
Fragments arranged by size:
5446 1014 516
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4784 276
Size: 53 83 186 936 3250 2468
Fragments arranged by size:
3250 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI