Restriction map for
pFN21AA1250
(Circular) (Six-base) MAPSORT of: pFN21AA1250.seq Check: 5450 from: 1 to: 10018
pFN21AA1250 10018 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5330 5330
Size: 10018
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7828 278
Size: 53 83 186 936 6292 2468
Fragments arranged by size:
6292 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6212 7361 1937
Size: 4275 1149 4594
Fragments arranged by size:
4594 4275 1149
Acc65I G'GTAC_C
Cuts at: 7339 7339
Size: 10018
AclI AA'CG_TT
Cuts at: 1563 7219 8145 8518 1563
Size: 5656 926 373 3063
Fragments arranged by size:
5656 3063 926 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3951 4343 4563 4992 5789 5880 5971 6456
Size: 2319 392 220 429 797 91 91 485
Cuts at: 6456 7014 8081 9465 9733 1632
Size: 558 1067 1384 268 1917
Fragments arranged by size:
2319 1917 1384 1067 797 558 485 429
392 268 220 91 91
AfeI AGC'GCT
Cuts at: 2865 2865
Size: 10018
AflII C'TTAA_G
Cuts at: 820 1017 6530 820
Size: 197 5513 4308
Fragments arranged by size:
5513 4308 197
AflIII A'CryG_T
Cuts at: 1203 3965 8917 9894 1203
Size: 2762 4952 977 1327
Fragments arranged by size:
4952 2762 1327 977
AgeI A'CCGG_T
Cuts at: 8849 8849
Size: 10018
AhdI GACnn_n'nnGTC
Cuts at: 8747 8747
Size: 10018
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10018
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2499 1184
Size: 1315 8703
AlwNI CAG_nnn'CTG
Cuts at: 2767 4366 7761 9333 2767
Size: 1599 3395 1572 3452
Fragments arranged by size:
3452 3395 1599 1572
ApaI G_GGCC'C
Cuts at: 5334 5396 6206 5334
Size: 62 810 9146
Fragments arranged by size:
9146 810 62
ApaLI G'TGCA_C
Cuts at: 2255 8075 9231 2255
Size: 5820 1156 3042
Fragments arranged by size:
5820 3042 1156
ApoI r'AATT_y
Cuts at: 1493 2667 4162 4565 5083 5626 5788 6117 7096
Size: 1174 1495 403 518 543 162 329 979
Cuts at: 7096 7327 7553 7705 9814 1493
Size: 231 226 152 2109 1697
Fragments arranged by size:
2109 1697 1495 1174 979 543 518 403
329 231 226 162 152
AseI AT'TA_AT
Cuts at: 160 1032 2174 8572 160
Size: 872 1142 6398 1606
Fragments arranged by size:
6398 1606 1142 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10018
AvaI C'yCGr_G
Cuts at: 1870 1943 5127 5584 6206 7343 1870
Size: 73 3184 457 622 1137 4545
Fragments arranged by size:
4545 3184 1137 622 457 73
AvrII C'CTAG_G
Cuts at: 5270 5270
Size: 10018
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5222 5255 5222
Size: 33 9985
BamHI G'GATC_C
Cuts at: 6424 7348 7715 6424
Size: 924 367 8727
Fragments arranged by size:
8727 924 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2384 5275 5802 6026 7339
Size: 325 217 627 597 2891 527 224 1313
Cuts at: 7339 8794 618
Size: 1455 1842
Fragments arranged by size:
2891 1842 1455 1313 627 597 527 325
224 217
BanII G_rGCy'C
Cuts at: 1391 5334 5396 5958 6206 6281 7260 7337 1391
Size: 3943 62 562 248 75 979 77 4072
Fragments arranged by size:
4072 3943 979 562 248 77 75 62
BbeI G_GCGC'C
Cuts at: 5279 5279
Size: 10018
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3450 5094 5868 6954 928
Size: 971 1551 1644 774 1086 3992
Fragments arranged by size:
3992 1644 1551 1086 971 774
BbvCI CC'TCA_GC
Cuts at: 3683 5397 5556 3683
Size: 1714 159 8145
Fragments arranged by size:
8145 1714 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3849 3883 8209 8243 1903
Size: 34 1912 34 4326 34 3678
Fragments arranged by size:
4326 3678 1912 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2806 4614 7910 9120 2806
Size: 1808 3296 1210 3704
Fragments arranged by size:
3704 3296 1808 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10018
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4268 6903 8629
Size: 107 122 71 631 605 2596 2635 1726
Cuts at: 8629 136
Size: 1525
Fragments arranged by size:
2635 2596 1726 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6230 10013 6230
Size: 3783 6235
BlpI GC'TnA_GC
Cuts at: 6684 7435 6684
Size: 751 9267
Bme1580I G_kGCm'C
Cuts at: 1790 2259 3343 5334 5396 6031 6206 8079 9235
Size: 469 1084 1991 62 635 175 1873 1156
Cuts at: 9235 1790
Size: 2573
Fragments arranged by size:
2573 1991 1873 1156 1084 635 469 175
62
BmgBI CAC'GTC
Cuts at: 1318 5319 5486 1318
Size: 4001 167 5850
Fragments arranged by size:
5850 4001 167
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4897 5108 5606
Size: 416 53 83 399 24 3480 211 498
Cuts at: 5606 6184 7749 8707 442
Size: 578 1565 958 1753
Fragments arranged by size:
3480 1753 1565 958 578 498 416 399
211 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4905 1056
Size: 3849 6169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2885 2885
Size: 10018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5944 6654 7059 8678 1239
Size: 4705 710 405 1619 2579
Fragments arranged by size:
4705 2579 1619 710 405
Bpu10I CC'TnA_GC
Cuts at: 3683 5397 5556 3683
Size: 1714 159 8145
Fragments arranged by size:
8145 1714 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3416 7116 8143 9008 9306 3416
Size: 3700 1027 865 298 4128
Fragments arranged by size:
4128 3700 1027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 8681 882
Size: 617 7182 2219
Fragments arranged by size:
7182 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5851 7809 493
Size: 711 4647 1958 2702
Fragments arranged by size:
4647 2702 1958 711
BsaBI GATnn'nnATC
Cuts at: 2592 10012 2592
Size: 7420 2598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5276 5969 7825 8207
Size: 53 83 186 936 3743 693 1856 382
Cuts at: 8207 8842 275
Size: 635 1451
Fragments arranged by size:
3743 1856 1451 936 693 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4935 7252 8451 8849 9123 9270 9681 4935
Size: 2317 1199 398 274 147 411 5272
Fragments arranged by size:
5272 2317 1199 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2574 2604 1178
Size: 30 165 30 1171 30 8592
Fragments arranged by size:
8592 1171 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3713 5572 1186
Size: 2527 1859 5632
Fragments arranged by size:
5632 2527 1859
BseYI C'CCAG_C
Cuts at: 5599 5896 6825 7043 9221 9717 5599
Size: 297 929 218 2178 496 5900
Fragments arranged by size:
5900 2178 929 496 297 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2520 2604 2780 4187 6161 6785 1163
Size: 573 784 84 176 1407 1974 624 4396
Fragments arranged by size:
4396 1974 1407 784 624 573 176 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7468 8229 9257 1540
Size: 275 186 5467 761 1028 2301
Fragments arranged by size:
5467 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 2301 5312 5958 7075 7260 7286 8079 8164
Size: 42 3011 646 1117 185 26 793 85
Cuts at: 8164 9235 2259
Size: 1071 3042
Fragments arranged by size:
3042 3011 1117 1071 793 646 185 85
42 26
BsmI GAATG_Cn'
Cuts at: 6974 7311 7529 7622 6974
Size: 337 218 93 9370
Fragments arranged by size:
9370 337 218 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4587 4587
Size: 10018
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2301 3343 5312 5334 5396 5958
Size: 399 469 42 1042 1969 22 62 562
Cuts at: 5958 6031 6206 6281 7075 7260 7286 7337 8079
Size: 73 175 75 794 185 26 51 742
Cuts at: 8079 8164 9235 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1969 1071 1042 794 742 562 469
399 185 175 85 75 73 62 51
42 26 22
BspEI T'CCGG_A
Cuts at: 7252 9681 7252
Size: 2429 7589
BspHI T'CATG_A
Cuts at: 4227 7907 9637 4227
Size: 3680 1730 4608
Fragments arranged by size:
4608 3680 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5330 7373 844
Size: 4486 2043 3489
Fragments arranged by size:
4486 3489 2043
BsrBI CCG'CTC
Cuts at: 1394 1823 5248 7905 1394
Size: 429 3425 2657 3507
Fragments arranged by size:
3507 3425 2657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4404 4561 6174 8513 8687 65
Size: 1183 3156 157 1613 2339 174 1396
Fragments arranged by size:
3156 2339 1613 1396 1183 174 157
BsrFI r'CCGG_y
Cuts at: 1700 1810 8662 8849 1700
Size: 110 6852 187 2869
Fragments arranged by size:
6852 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2409 96
Size: 2313 7705
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10018
BssSI C'ACGA_G
Cuts at: 8078 9090 8078
Size: 1012 9006
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2767 794
Size: 1973 8045
BstBI TT'CG_AA
Cuts at: 5994 8822 5994
Size: 2828 7190
BstEII G'GTnAC_C
Cuts at: 1184 4780 1184
Size: 3596 6422
BstXI CCAn_nnnn'nTGG
Cuts at: 2430 3388 2430
Size: 958 9060
BstYI r'GATC_y
Cuts at: 1972 2764 5167 6230 6424 6770 6913 7246 7348
Size: 792 2403 1063 194 346 143 333 102
Cuts at: 7348 7715 8100 8117 9569 9655 9667 10013 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2403 1977 1452 1063 792 385 367 346
346 333 194 143 102 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2577 513
Size: 552 1512 7954
Fragments arranged by size:
7954 1512 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9279
Fragments arranged by size:
9279 683 56
BtsI GCAGTG_nn'
Cuts at: 2640 3343 3591 4931 5896 6608 7534 8328 8348
Size: 703 248 1340 965 712 926 794 20
Cuts at: 8348 2640
Size: 4310
Fragments arranged by size:
4310 1340 965 926 794 712 703 248
20
ClaI AT'CG_AT
Cuts at: 1554 4323 6378 1554
Size: 2769 2055 5194
Fragments arranged by size:
5194 2769 2055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3738 3773 4293 4328 529
Size: 35 3174 35 520 35 6219
Fragments arranged by size:
6219 3174 520 35 35 35
DraI TTT'AAA
Cuts at: 7322 7322
Size: 10018
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9025 809
Size: 507 7709 1802
Fragments arranged by size:
7709 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2574 4260
Size: 54 1425 50 275 18 286 458 1686
Cuts at: 4260 4492 4928 5734 7465 8354 9983 8
Size: 232 436 806 1731 889 1629 43
Fragments arranged by size:
1731 1686 1629 1425 889 806 458 436
286 275 232 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7465 1537
Size: 275 5653 4090
Fragments arranged by size:
5653 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2079 2669 3245 3643 4035 4636 4959 5317
Size: 726 590 576 398 392 601 323 358
Cuts at: 5317 5991 6356 6515 7279 7948 1353
Size: 674 365 159 764 669 3423
Fragments arranged by size:
3423 764 726 674 669 601 590 576
398 392 365 358 323 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6830 8605 8979 9125 387
Size: 1064 138 5241 1775 374 146 1280
Fragments arranged by size:
5241 1775 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5956 7258 5956
Size: 1302 8716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3951 4343 4563 4992 5789 5880 5944
Size: 393 2319 392 220 429 797 91 64
Cuts at: 5944 5971 6456 6654 7014 7059 8081 8678 9465
Size: 27 485 198 360 45 1022 597 787
Cuts at: 9465 9733 1239
Size: 268 1524
Fragments arranged by size:
2319 1524 1022 797 787 597 485 429
393 392 360 268 220 198 91 64
45 27
EcoO109I rG'GnC_Cy
Cuts at: 3679 5109 5264 5331 5393 6202 6203 3679
Size: 1430 155 67 62 809 1 7494
Fragments arranged by size:
7494 1430 809 155 67 62 1
EcoRI G'AATT_C
Cuts at: 4162 5626 7327 7705 4162
Size: 1464 1701 378 6475
Fragments arranged by size:
6475 1701 1464 378
EcoRV GAT'ATC
Cuts at: 2019 2965 2019
Size: 946 9072
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2093 2093
Size: 10018
FspI TGC'GCA
Cuts at: 4508 8524 4508
Size: 4016 6002
HaeII r_GCGC'y
Cuts at: 1678 2832 2867 5279 9165 1678
Size: 1154 35 2412 3886 2531
Fragments arranged by size:
3886 2531 2412 1154 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3466 3498 4729 4761 4855
Size: 32 135 32 1894 32 1231 32 94
Cuts at: 4855 4887 5202 5234 5380 5412 6360 6371 6392
Size: 32 315 32 146 32 948 11 21
Cuts at: 6392 6403 6434 6466 6563 6595 6707 6739 8659
Size: 11 31 32 97 32 112 32 1920
Cuts at: 8659 8691 8733 8765 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1920 1894 1231 948 315 146 135
112 97 94 42 32 32 32 32
32 32 32 32 32 32 32 32
31 21 11 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2290 2494 4051 7362 7608 669
Size: 988 281 352 204 1557 3311 246 3079
Fragments arranged by size:
3311 3079 1557 988 352 281 246 204
HindIII A'AGCT_T
Cuts at: 748 3777 6125 6470 6527 748
Size: 3029 2348 345 57 4239
Fragments arranged by size:
4239 3029 2348 345 57
HpaI GTT'AAC
Cuts at: 2290 7608 2290
Size: 5318 4700
KasI G'GCGC_C
Cuts at: 5275 5275
Size: 10018
KpnI G_GTAC'C
Cuts at: 7343 7343
Size: 10018
MfeI C'AATT_G
Cuts at: 7617 7617
Size: 10018
MluI A'CGCG_T
Cuts at: 9894 9894
Size: 10018
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3644 5057 9132 9316 1305
Size: 2339 1413 4075 184 2007
Fragments arranged by size:
4075 2339 2007 1413 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2118 2576 4262 4494 4930
Size: 54 1425 343 286 458 1686 232 436
Cuts at: 4930 10
Size: 5098
Fragments arranged by size:
5098 1686 1425 458 436 343 286 232
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3050 3386 5305 7976 8335 8494 518
Size: 648 1884 336 1919 2671 359 159 2042
Fragments arranged by size:
2671 2042 1919 1884 648 359 336 159
MspA1I CmG'CkG
Cuts at: 1606 5382 6873 7434 8111 9259 9504 1606
Size: 3776 1491 561 677 1148 245 2120
Fragments arranged by size:
3776 2120 1491 1148 677 561 245
NarI GG'CG_CC
Cuts at: 5276 5276
Size: 10018
NcoI C'CATG_G
Cuts at: 513 1065 2577 513
Size: 552 1512 7954
Fragments arranged by size:
7954 1512 552
NdeI CA'TA_TG
Cuts at: 387 2785 387
Size: 2398 7620
NheI G'CTAG_C
Cuts at: 1052 4901 1052
Size: 3849 6169
NotI GC'GGCC_GC
Cuts at: 7465 7465
Size: 10018
NspI r_CATG'y
Cuts at: 1132 1229 2255 2340 3316 3969 4507 7014 7376
Size: 97 1026 85 976 653 538 2507 362
Cuts at: 7376 8921 1132
Size: 1545 2229
Fragments arranged by size:
2507 2229 1545 1026 976 653 538 362
97 85
PacI TTA_AT'TAA
Cuts at: 2178 2178
Size: 10018
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10018
PciI A'CATG_T
Cuts at: 3965 8917 3965
Size: 4952 5066
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3575 1108
Size: 2467 7551
PmeI GTTT'AAAC
Cuts at: 7322 7322
Size: 10018
PmlI CAC'GTG
Cuts at: 5851 5851
Size: 10018
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4778 4810 7135 7167 8087 8119 9600 9632 4778
Size: 32 2325 32 920 32 1481 32 5164
Fragments arranged by size:
5164 2325 1481 920 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3679 5109 5264 3679
Size: 1430 155 8433
Fragments arranged by size:
8433 1430 155
PshAI GACnn'nnGTC
Cuts at: 1613 3286 4806 1613
Size: 1673 1520 6825
Fragments arranged by size:
6825 1673 1520
PsiI TTA'TAA
Cuts at: 3023 3228 7588 3023
Size: 205 4360 5453
Fragments arranged by size:
5453 4360 205
PspOMI G'GGCC_C
Cuts at: 5330 5392 6202 5330
Size: 62 810 9146
Fragments arranged by size:
9146 810 62
PspXI vC'TCGA_Gb
Cuts at: 5584 5584
Size: 10018
PstI C_TGCA'G
Cuts at: 830 7370 830
Size: 6540 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10018
SacI G_AGCT'C
Cuts at: 5958 7260 5958
Size: 1302 8716
SalI G'TCGA_C
Cuts at: 1936 7360 1936
Size: 5424 4594
SapI GCTCTTCn'nnn_
Cuts at: 2079 5317 5991 2079
Size: 3238 674 6106
Fragments arranged by size:
6106 3238 674
SbfI CC_TGCA'GG
Cuts at: 7370 7370
Size: 10018
ScaI AGT'ACT
Cuts at: 5823 8266 9978 5823
Size: 2443 1712 5863
Fragments arranged by size:
5863 2443 1712
SfcI C'TryA_G
Cuts at: 826 1045 3093 3238 4098 4614 6163 7366 8501
Size: 219 2048 145 860 516 1549 1203 1135
Cuts at: 8501 9182 9373 826
Size: 681 191 1471
Fragments arranged by size:
2048 1549 1471 1203 1135 860 681 516
219 191 145
SfoI GGC'GCC
Cuts at: 5277 5277
Size: 10018
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10018
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10018
SmaI CCC'GGG
Cuts at: 1872 6208 7345 1872
Size: 4336 1137 4545
Fragments arranged by size:
4545 4336 1137
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3431 5127 5584 6530 7131 8122
Size: 197 926 1488 1696 457 946 601 991
Cuts at: 8122 9023 9285 820
Size: 901 262 1553
Fragments arranged by size:
1696 1553 1488 991 946 926 901 601
457 262 197
SnaBI TAC'GTA
Cuts at: 493 7809 493
Size: 7316 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10018
SphI G_CATG'C
Cuts at: 1132 7014 7376 1132
Size: 5882 362 3774
Fragments arranged by size:
5882 3774 362
SspI AAT'ATT
Cuts at: 5 52 3232 3270 4837 5029 7942 5
Size: 47 3180 38 1567 192 2913 2081
Fragments arranged by size:
3180 2913 2081 1567 192 47 38
StuI AGG'CCT
Cuts at: 1517 7147 1517
Size: 5630 4388
StyI C'CwwG_G
Cuts at: 513 1065 2577 4660 5270 5447 7457 513
Size: 552 1512 2083 610 177 2010 3074
Fragments arranged by size:
3074 2083 2010 1512 610 552 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3394 3582 5587 8045 8062 8215 8400
Size: 1255 1814 188 2005 2458 17 153 185
Cuts at: 8400 8845 325
Size: 445 1498
Fragments arranged by size:
2458 2005 1814 1498 1255 445 188 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2409 5821 8264
Size: 274 80 33 51 1443 432 3412 2443
Cuts at: 8264 9976 96
Size: 1712 138
Fragments arranged by size:
3412 2443 1712 1443 432 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2574 2606 2772 2804 3570 3602 6304 6336 9978
Size: 32 166 32 766 32 2702 32 3642
Cuts at: 9978 10010 2574
Size: 32 2582
Fragments arranged by size:
3642 2702 2582 766 166 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4352 4373 1316
Size: 3036 21 6961
Fragments arranged by size:
6961 3036 21
XbaI T'CTAG_A
Cuts at: 7354 7354
Size: 10018
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3572 3572
Size: 10018
XhoI C'TCGA_G
Cuts at: 1943 5127 5584 1943
Size: 3184 457 6377
Fragments arranged by size:
6377 3184 457
XmaI C'CCGG_G
Cuts at: 1870 6206 7343 1870
Size: 4336 1137 4545
Fragments arranged by size:
4545 4336 1137
XmnI GAAnn'nnTTC
Cuts at: 2055 2856 3422 3458 4645 4977 7311 8147 2055
Size: 801 566 36 1187 332 2334 836 3926
Fragments arranged by size:
3926 2334 1187 836 801 566 332 36
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7826 276
Size: 53 83 186 936 6292 2468
Fragments arranged by size:
6292 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI NaeI NgoMIV NruI NsiI PfoI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI