Restriction map for
pFN21AA1248
(Circular) (Six-base) MAPSORT of: pFN21AA1248.seq Check: 9414 from: 1 to: 5776
pFN21AA1248 5776 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3839 5223 5491 1632
Size: 2207 1384 268 1917
Fragments arranged by size:
2207 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 2988 4607 2988
Size: 1619 4157
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 2206 3519 5091 2206
Size: 1313 1572 2891
Fragments arranged by size:
2891 1572 1313
ApaI G_GGCC'C
Cuts at: 3048 3048
Size: 5776
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 2368 2590 3085 3311 3463 5572 1493
Size: 875 222 495 226 152 2109 1697
Fragments arranged by size:
2109 1697 875 495 226 222 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 3049 3101 1870
Size: 73 1106 52 4545
Fragments arranged by size:
4545 1106 73 52
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2594 2627 2594
Size: 33 5743
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2129 3097 4552 618
Size: 325 217 627 342 968 1455 1842
Fragments arranged by size:
1842 1455 968 627 342 325 217
BanII G_rGCy'C
Cuts at: 1391 2290 2583 3048 3095 1391
Size: 899 293 465 47 4072
Fragments arranged by size:
4072 899 465 293 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3052 4387 136
Size: 107 122 71 631 605 1380 1335 1525
Fragments arranged by size:
1525 1380 1335 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 3048 3057 3837 4993 1790
Size: 1258 9 780 1156 2573
Fragments arranged by size:
2573 1258 1156 780 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2445 2445
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2424 4436 1239
Size: 1185 2012 2579
Fragments arranged by size:
2579 2012 1185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2804 3901 4766 5064 2804
Size: 1097 865 298 3516
Fragments arranged by size:
3516 1097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2679 2742 2949 3073 4439 882
Size: 617 1180 63 207 124 1366 2219
Fragments arranged by size:
2219 1366 1180 617 207 124 63
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2063 3583 3965 4600
Size: 53 83 186 936 530 1520 382 635
Cuts at: 4600 275
Size: 1451
Fragments arranged by size:
1520 1451 936 635 530 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2542 2834 2988 4209 4607 4881 5028 5439 2542
Size: 292 154 1221 398 274 147 411 2879
Fragments arranged by size:
2879 1221 411 398 292 274 154 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2277 2307 2379 2409 1178
Size: 30 165 30 874 30 72 30 4545
Fragments arranged by size:
4545 874 165 72 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 1186
Size: 865 4911
BseYI C'CCAG_C
Cuts at: 2149 2847 4979 5475 2149
Size: 698 2132 496 2450
Fragments arranged by size:
2450 2132 698 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2045 2988 1163
Size: 573 309 943 3951
Fragments arranged by size:
3951 943 573 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2583 3837 3922 4993 2583
Size: 1254 85 1071 3366
Fragments arranged by size:
3366 1254 1071 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2290 2583 3048 3057 3095 3837 3922
Size: 399 500 293 465 9 38 742 85
Cuts at: 3922 4993 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 500 465 399 293 85
38 9
BspEI T'CCGG_A
Cuts at: 2542 2834 5439 2542
Size: 292 2605 2879
Fragments arranged by size:
2879 2605 292
BspHI T'CATG_A
Cuts at: 2761 3665 5395 2761
Size: 904 1730 3142
Fragments arranged by size:
3142 1730 904
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3131 844
Size: 2287 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2997 3663 1394
Size: 429 1174 666 3507
Fragments arranged by size:
3507 1174 666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2988 4420 4607 1700
Size: 110 1178 1432 187 2869
Fragments arranged by size:
2869 1432 1178 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2023 794
Size: 1229 4547
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771
Size: 1134 367 385 17 1452 86 12 346
Cuts at: 5771 1972
Size: 1977
Fragments arranged by size:
1977 1452 1134 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2067 2720 2067
Size: 653 5123
BtgI C'CryG_G
Cuts at: 513 1065 2004 3060 513
Size: 552 939 1056 3229
Fragments arranged by size:
3229 1056 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2778 3292 4086 4106 2778
Size: 514 794 20 4448
Fragments arranged by size:
4448 794 514 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DraIII CAC_nnn'GTG
Cuts at: 2089 2173 2089
Size: 84 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2935 4783 809
Size: 507 1619 1848 1802
Fragments arranged by size:
1848 1802 1619 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 3223 4112
Size: 54 1425 50 275 18 243 1150 889
Cuts at: 4112 5741 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1150 889 275 243 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2274 2539 2571 3706 1353
Size: 921 265 32 1135 3423
Fragments arranged by size:
3423 1135 921 265 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4363 4737 4883 387
Size: 1064 138 435 2339 374 146 1280
Fragments arranged by size:
2339 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2581 2581
Size: 5776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2424 3839 4436 5223 5491 1239
Size: 393 792 1415 597 787 268 1524
Fragments arranged by size:
1524 1415 792 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3044 3045 3044
Size: 1 5775
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
EcoRV GAT'ATC
Cuts at: 2309 2713 2309
Size: 404 5372
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 4923 1678
Size: 3245 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2397 2429 2697 2729 4417
Size: 32 135 32 825 32 268 32 1688
Cuts at: 4417 4449 4491 4523 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1688 825 268 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2377 2474 4890 5074 1305
Size: 1072 97 2416 184 2007
Fragments arranged by size:
2416 2007 1072 184 97
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2262 2766 3734 4093 4252 518
Size: 648 1096 504 968 359 159 2042
Fragments arranged by size:
2042 1096 968 648 504 359 159
MspA1I CmG'CkG
Cuts at: 1606 2851 3192 3869 5017 5262 1606
Size: 1245 341 677 1148 245 2120
Fragments arranged by size:
2120 1245 1148 677 341 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3060 513
Size: 552 939 1056 3229
Fragments arranged by size:
3229 1056 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2227 2633 3134 4679 1132
Size: 97 998 406 501 1545 2229
Fragments arranged by size:
2229 1545 998 501 406 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2173 2493 1108
Size: 1065 320 4391
Fragments arranged by size:
4391 1065 320
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspOMI G'GGCC_C
Cuts at: 3044 3044
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2019 2361 3128 830
Size: 1189 342 767 3478
Fragments arranged by size:
3478 1189 767 342
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2851 2851
Size: 5776
SacI G_AGCT'C
Cuts at: 2583 2583
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2571 2571
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 2888 4024 5736 2888
Size: 1136 1712 2928
Fragments arranged by size:
2928 1712 1136
SfcI C'TryA_G
Cuts at: 826 1045 2015 2357 3124 4259 4940 5131 826
Size: 219 970 342 767 1135 681 191 1471
Fragments arranged by size:
1471 1135 970 767 681 342 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3051 3103 1872
Size: 1179 52 4545
Fragments arranged by size:
4545 1179 52
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2819 3880 4781 5043 820
Size: 197 926 876 1061 901 262 1553
Fragments arranged by size:
1553 1061 926 901 876 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 2526 2879 1517
Size: 1009 353 4414
Fragments arranged by size:
4414 1009 353
StyI C'CwwG_G
Cuts at: 513 1065 2004 2487 2554 3060 3215 513
Size: 552 939 483 67 506 155 3074
Fragments arranged by size:
3074 939 552 506 483 155 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2650 2819 2888 3803 3820 3973 4158
Size: 1255 1070 169 69 915 17 153 185
Cuts at: 4158 4603 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1070 915 445 185 169 153
69 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2610 2658 2886
Size: 274 80 33 51 1443 633 48 228
Cuts at: 2886 4022 5734 96
Size: 1136 1712 138
Fragments arranged by size:
1712 1443 1136 633 274 228 138 80
51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2277 2309 5736 5768 2277
Size: 32 3427 32 2285
Fragments arranged by size:
3427 2285 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2494 2494
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3049 3101 1870
Size: 1179 52 4545
Fragments arranged by size:
4545 1179 52
XmnI GAAnn'nnTTC
Cuts at: 2235 3034 3905 2235
Size: 799 871 4106
Fragments arranged by size:
4106 871 799
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI