Restriction map for
pFN21AA1231
(Circular) (Six-base) MAPSORT of: pFN21AA1231.seq Check: 136 from: 1 to: 7888
pFN21AA1231 7888 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3201 3605 5080 3201
Size: 404 1475 6009
Fragments arranged by size:
6009 1475 404
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5698 278
Size: 53 83 186 936 4162 2468
Fragments arranged by size:
4162 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2953 5231 1937
Size: 1016 2278 4594
Fragments arranged by size:
4594 2278 1016
Acc65I G'GTAC_C
Cuts at: 5209 5209
Size: 7888
AclI AA'CG_TT
Cuts at: 1563 6015 6388 1563
Size: 4452 373 3063
Fragments arranged by size:
4452 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2280 2729 3284 4142 5951 7335 7603 1632
Size: 648 449 555 858 1809 1384 268 1917
Fragments arranged by size:
1917 1809 1384 858 648 555 449 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7691
AflIII A'CryG_T
Cuts at: 1203 3646 4842 6787 7764 1203
Size: 2443 1196 1945 977 1327
Fragments arranged by size:
2443 1945 1327 1196 977
AgeI A'CCGG_T
Cuts at: 6719 6719
Size: 7888
AhdI GACnn_n'nnGTC
Cuts at: 6617 6617
Size: 7888
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7888
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5055 5186 1184
Size: 3871 131 3886
Fragments arranged by size:
3886 3871 131
AlwNI CAG_nnn'CTG
Cuts at: 2068 2638 2707 3307 4798 5631 7203 2068
Size: 570 69 600 1491 833 1572 2753
Fragments arranged by size:
2753 1572 1491 833 600 570 69
ApaLI G'TGCA_C
Cuts at: 3682 5945 7101 3682
Size: 2263 1156 4469
Fragments arranged by size:
4469 2263 1156
ApoI r'AATT_y
Cuts at: 1493 2754 2975 4515 4825 4864 5197 5423 5575
Size: 1261 221 1540 310 39 333 226 152
Cuts at: 5575 7684 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1540 1261 333 310 226 221
152 39
AseI AT'TA_AT
Cuts at: 160 1032 6442 160
Size: 872 5410 1606
Fragments arranged by size:
5410 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7888
AvaI C'yCGr_G
Cuts at: 1870 1943 2352 5213 1870
Size: 73 409 2861 4545
Fragments arranged by size:
4545 2861 409 73
BamHI G'GATC_C
Cuts at: 5218 5585 5218
Size: 367 7521
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3220 4018 4226 5209 6664
Size: 325 217 627 1433 798 208 983 1455
Cuts at: 6664 618
Size: 1842
Fragments arranged by size:
1842 1455 1433 983 798 627 325 217
208
BanII G_rGCy'C
Cuts at: 1391 2477 2697 4146 4752 5207 1391
Size: 1086 220 1449 606 455 4072
Fragments arranged by size:
4072 1449 1086 606 455 220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 2559 3147 3509 928
Size: 971 303 357 588 362 5307
Fragments arranged by size:
5307 971 588 362 357 303
BbvCI CC'TCA_GC
Cuts at: 3132 4563 4659 4726 3132
Size: 1431 96 67 6294
Fragments arranged by size:
6294 1431 96 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4420 4454 5054 5088 6079 6113 1903
Size: 34 2483 34 600 34 991 34 3678
Fragments arranged by size:
3678 2483 991 600 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5780 6990 5780
Size: 1210 6678
BclI T'GATC_A
Cuts at: 1548 4371 1548
Size: 2823 5065
BfrBI ATG'CAT
Cuts at: 3829 3829
Size: 7888
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4736 6499 136
Size: 107 122 71 631 605 3064 1763 1525
Fragments arranged by size:
3064 1763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3672 7883 3672
Size: 4211 3677
BlpI GC'TnA_GC
Cuts at: 4202 4740 4751 5305 4202
Size: 538 11 554 6785
Fragments arranged by size:
6785 554 538 11
Bme1580I G_kGCm'C
Cuts at: 1790 3225 3686 4229 5949 7105 1790
Size: 1435 461 543 1720 1156 2573
Fragments arranged by size:
2573 1720 1435 1156 543 461
BmgBI CAC'GTC
Cuts at: 1318 3900 1318
Size: 2582 5306
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2233 2434 3145
Size: 416 53 83 399 24 816 201 711
Cuts at: 3145 4848 5619 6577 442
Size: 1703 771 958 1753
Fragments arranged by size:
1753 1703 958 816 771 711 416 399
201 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7888
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4160
Size: 7888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2648 4203 4434 4538 4935 6548 1239
Size: 917 492 1555 231 104 397 1613 2579
Fragments arranged by size:
2579 1613 1555 917 492 397 231 104
Bpu10I CC'TnA_GC
Cuts at: 3132 4563 4659 4726 3132
Size: 1431 96 67 6294
Fragments arranged by size:
6294 1431 96 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2489 6013 6878 7176 2489
Size: 3524 865 298 3201
Fragments arranged by size:
3524 3201 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6551 882
Size: 617 5052 2219
Fragments arranged by size:
5052 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3649 4845 5679 493
Size: 711 2445 1196 834 2702
Fragments arranged by size:
2702 2445 1196 834 711
BsaBI GATnn'nnATC
Cuts at: 7882 7882
Size: 7888
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2965 4544 5695 6077
Size: 53 83 186 936 1432 1579 1151 382
Cuts at: 6077 6712 275
Size: 635 1451
Fragments arranged by size:
1579 1451 1432 1151 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6321 6719 6993 7140 7551 6321
Size: 398 274 147 411 6658
Fragments arranged by size:
6658 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3711 3741 4407 4437 4894
Size: 30 165 30 2308 30 666 30 457
Cuts at: 4894 4924 5046 5069 5076 5099 1178
Size: 30 122 23 7 23 3967
Fragments arranged by size:
3967 2308 666 457 165 122 30 30
30 30 30 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 1186
Size: 1102 6786
BseYI C'CCAG_C
Cuts at: 4215 4329 4384 4671 4731 7091 7587 4215
Size: 114 55 287 60 2360 496 4516
Fragments arranged by size:
4516 2360 496 287 114 60 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3173 4709 4856 5058 1163
Size: 573 1437 1536 147 202 3993
Fragments arranged by size:
3993 1536 1437 573 202 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2342 2960 3862 5338 6099 7127
Size: 275 186 341 618 902 1476 761 1028
Cuts at: 7127 1540
Size: 2301
Fragments arranged by size:
2301 1476 1028 902 761 618 341 275
186
BsiHKAI G_wGCw'C
Cuts at: 2349 2382 3236 3686 4146 4208 5949 6034 7105
Size: 33 854 450 460 62 1741 85 1071
Cuts at: 7105 2349
Size: 3132
Fragments arranged by size:
3132 1741 1071 854 460 450 85 62
33
BsmI GAATG_Cn'
Cuts at: 3295 3742 4155 5399 5492 3295
Size: 447 413 1244 93 5691
Fragments arranged by size:
5691 1244 447 413 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2573 2573
Size: 7888
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2349 2382 2477 2697 3225 3236 3686
Size: 399 559 33 95 220 528 11 450
Cuts at: 3686 4146 4208 4229 4752 5207 5949 6034 7105
Size: 460 62 21 523 455 742 85 1071
Cuts at: 7105 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 559 528 523 460 455
450 399 220 95 85 62 33 21
11
BspEI T'CCGG_A
Cuts at: 7551 7551
Size: 7888
BspHI T'CATG_A
Cuts at: 2758 2875 5777 7507 2758
Size: 117 2902 1730 3139
Fragments arranged by size:
3139 2902 1730 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3201 3605 4446 4857 5080 5243 844
Size: 2357 404 841 411 223 163 3489
Fragments arranged by size:
3489 2357 841 411 404 223 163
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 5775 1394
Size: 429 2050 1902 3507
Fragments arranged by size:
3507 2050 1902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2045 4458 6383 6557 65
Size: 1183 797 2413 1925 174 1396
Fragments arranged by size:
2413 1925 1396 1183 797 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2337 2341 6532 6719 1700
Size: 110 527 4 4191 187 2869
Fragments arranged by size:
4191 2869 527 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7888
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7888
BssSI C'ACGA_G
Cuts at: 5948 6960 5948
Size: 1012 6876
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2732 3522 3826 4798 794
Size: 1938 790 304 972 3884
Fragments arranged by size:
3884 1938 972 790 304
BstBI TT'CG_AA
Cuts at: 4306 6692 4306
Size: 2386 5502
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7888
BstXI CCAn_nnnn'nTGG
Cuts at: 4706 4706
Size: 7888
BstYI r'GATC_y
Cuts at: 1972 2212 3672 5218 5585 5970 5987 7439 7525
Size: 240 1460 1546 367 385 17 1452 86
Cuts at: 7525 7537 7883 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1546 1460 1452 385 367 346 240
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2451 3400 4873 513
Size: 552 1386 949 1473 3528
Fragments arranged by size:
3528 1473 1386 949 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4216 487
Size: 683 56 2990 4159
Fragments arranged by size:
4159 2990 683 56
BtsI GCAGTG_nn'
Cuts at: 3353 4258 5404 6198 6218 3353
Size: 905 1146 794 20 5023
Fragments arranged by size:
5023 1146 905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2244 2279 529
Size: 35 1680 35 6138
Fragments arranged by size:
6138 1680 35 35
DraI TTT'AAA
Cuts at: 3337 5192 3337
Size: 1855 6033
DraIII CAC_nnn'GTG
Cuts at: 2665 3690 5081 2665
Size: 1025 1391 5472
Fragments arranged by size:
5472 1391 1025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4176 4462 6895 809
Size: 507 2860 286 2433 1802
Fragments arranged by size:
2860 2433 1802 507 286
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2339 2609 2656
Size: 54 1425 50 275 18 509 270 47
Cuts at: 2656 2957 3567 4599 4642 4963 5335 6224 7853
Size: 301 610 1032 43 321 372 889 1629
Cuts at: 7853 8
Size: 43
Fragments arranged by size:
1629 1425 1032 889 610 509 372 321
301 275 270 54 50 47 43 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2339 2957 5335 1537
Size: 275 527 618 2378 4090
Fragments arranged by size:
4090 2378 618 527 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 3204 3303 3983 4008 5818 1353
Size: 792 1059 99 680 25 1810 3423
Fragments arranged by size:
3423 1810 1059 792 680 99 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2972 6475 6849 6995 387
Size: 1064 138 1383 3503 374 146 1280
Fragments arranged by size:
3503 1383 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4144 4144
Size: 7888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2280 2648 2729 3284 4142 4203
Size: 393 524 124 368 81 555 858 61
Cuts at: 4203 4434 4538 4935 5951 6548 7335 7603 1239
Size: 231 104 397 1016 597 787 268 1524
Fragments arranged by size:
1524 1016 858 787 597 555 524 397
393 368 268 231 124 104 81 61
EcoO109I rG'GnC_Cy
Cuts at: 2459 2459
Size: 7888
EcoRI G'AATT_C
Cuts at: 4515 4825 4864 5197 5575 4515
Size: 310 39 333 378 6828
Fragments arranged by size:
6828 378 333 310 39
FspI TGC'GCA
Cuts at: 3257 3653 6394 3257
Size: 396 2741 4751
Fragments arranged by size:
4751 2741 396
HaeII r_GCGC'y
Cuts at: 1678 4410 7035 1678
Size: 2732 2625 2531
Fragments arranged by size:
2732 2625 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3418 3450 3817 3849 4894
Size: 32 135 32 1846 32 367 32 1045
Cuts at: 4894 4926 5046 5078 6529 6561 6603 6635 1373
Size: 32 120 32 1451 32 42 32 2626
Fragments arranged by size:
2626 1846 1451 1045 367 135 120 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2954 4029 5232 5478 669
Size: 988 281 1016 1075 1203 246 3079
Fragments arranged by size:
3079 1203 1075 1016 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7888
HpaI GTT'AAC
Cuts at: 4029 5478 4029
Size: 1449 6439
KpnI G_GTAC'C
Cuts at: 5213 5213
Size: 7888
MfeI C'AATT_G
Cuts at: 5487 5487
Size: 7888
MluI A'CGCG_T
Cuts at: 7764 7764
Size: 7888
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2121 4192 4362 7002 7186 1305
Size: 816 2071 170 2640 184 2007
Fragments arranged by size:
2640 2071 2007 816 184 170
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2658 3569 4644 4965 10
Size: 54 1425 343 826 911 1075 321 2933
Fragments arranged by size:
2933 1425 1075 911 826 343 321 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3579 4529 4685 5846 6205 6364 518
Size: 648 2413 950 156 1161 359 159 2042
Fragments arranged by size:
2413 2042 1161 950 648 359 159 156
MspA1I CmG'CkG
Cuts at: 1606 3136 4175 4219 4367 4388 4576 5304 5981
Size: 1530 1039 44 148 21 188 728 677
Cuts at: 5981 7129 7374 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1530 1148 1039 728 677 245 188
148 44 21
NcoI C'CATG_G
Cuts at: 513 1065 3400 513
Size: 552 2335 5001
Fragments arranged by size:
5001 2335 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7888
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7888
NotI GC'GGCC_GC
Cuts at: 5335 5335
Size: 7888
NsiI A_TGCA'T
Cuts at: 3831 3831
Size: 7888
NspI r_CATG'y
Cuts at: 1132 1229 2294 3256 3736 3820 3970 5246 6791
Size: 97 1065 962 480 84 150 1276 1545
Cuts at: 6791 1132
Size: 2229
Fragments arranged by size:
2229 1545 1276 1065 962 480 150 97
84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7888
PciI A'CATG_T
Cuts at: 6787 6787
Size: 7888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4860 1108
Size: 3752 4136
PfoI T'CCnGG_A
Cuts at: 3363 4180 3363
Size: 817 7071
PmeI GTTT'AAAC
Cuts at: 5192 5192
Size: 7888
PmlI CAC'GTG
Cuts at: 3649 3649
Size: 7888
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5957 5989 7470 7502 5957
Size: 32 1481 32 6343
Fragments arranged by size:
6343 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7888
PsiI TTA'TAA
Cuts at: 2840 4089 5458 2840
Size: 1249 1369 5270
Fragments arranged by size:
5270 1369 1249
PstI C_TGCA'G
Cuts at: 830 2118 3198 3288 4803 5240 830
Size: 1288 1080 90 1515 437 3478
Fragments arranged by size:
3478 1515 1288 1080 437 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7888
PvuII CAG'CTG
Cuts at: 3136 4219 4367 4576 3136
Size: 1083 148 209 6448
Fragments arranged by size:
6448 1083 209 148
RsrII CG'GwC_CG
Cuts at: 3748 3748
Size: 7888
SacI G_AGCT'C
Cuts at: 4146 4146
Size: 7888
SalI G'TCGA_C
Cuts at: 1936 2952 5230 1936
Size: 1016 2278 4594
Fragments arranged by size:
4594 2278 1016
SapI GCTCTTCn'nnn_
Cuts at: 3303 3303
Size: 7888
SbfI CC_TGCA'GG
Cuts at: 5240 5240
Size: 7888
ScaI AGT'ACT
Cuts at: 6136 7848 6136
Size: 1712 6176
SexAI A'CCwGG_T
Cuts at: 2061 2637 2061
Size: 576 7312
SfcI C'TryA_G
Cuts at: 826 1045 2114 3194 3284 4398 4799 5236 6371
Size: 219 1069 1080 90 1114 401 437 1135
Cuts at: 6371 7052 7243 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1114 1080 1069 681 437 401
219 191 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7888
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7888
SmaI CCC'GGG
Cuts at: 1872 2354 5215 1872
Size: 482 2861 4545
Fragments arranged by size:
4545 2861 482
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2504 5992 6893 7155 820
Size: 197 926 561 3488 901 262 1553
Fragments arranged by size:
3488 1553 926 901 561 262 197
SnaBI TAC'GTA
Cuts at: 493 5679 493
Size: 5186 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7888
SphI G_CATG'C
Cuts at: 1132 2294 3970 5246 1132
Size: 1162 1676 1276 3774
Fragments arranged by size:
3774 1676 1276 1162
SspI AAT'ATT
Cuts at: 5 52 5812 5
Size: 47 5760 2081
Fragments arranged by size:
5760 2081 47
StuI AGG'CCT
Cuts at: 1517 2042 3359 3701 1517
Size: 525 1317 342 5704
Fragments arranged by size:
5704 1317 525 342
StyI C'CwwG_G
Cuts at: 513 1065 3400 3468 5327 513
Size: 552 2335 68 1859 3074
Fragments arranged by size:
3074 2335 1859 552 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2453 2933 3242 4011 4146 4229 4460
Size: 1255 873 480 309 769 135 83 231
Cuts at: 4460 5915 5932 6085 6270 6715 325
Size: 1455 17 153 185 445 1498
Fragments arranged by size:
1498 1455 1255 873 769 480 445 309
231 185 153 135 83 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4272 4521 5127
Size: 274 80 33 51 1443 2295 249 606
Cuts at: 5127 6134 7846 96
Size: 1007 1712 138
Fragments arranged by size:
2295 1712 1443 1007 606 274 249 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4407 4439 5069 5101 7848 7880 4407
Size: 32 630 32 2747 32 4415
Fragments arranged by size:
4415 2747 630 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7888
XbaI T'CTAG_A
Cuts at: 5224 5224
Size: 7888
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2262 2686 2262
Size: 424 7464
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7888
XmaI C'CCGG_G
Cuts at: 1870 2352 5213 1870
Size: 482 2861 4545
Fragments arranged by size:
4545 2861 482
XmnI GAAnn'nnTTC
Cuts at: 3323 3359 3617 4868 6017 3323
Size: 36 258 1251 1149 5194
Fragments arranged by size:
5194 1251 1149 258 36
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5696 276
Size: 53 83 186 936 4162 2468
Fragments arranged by size:
4162 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI
PsrI SacII SanDI SfiI SfoI SrfI SwaI