Restriction map for
pFN21AA1230
(Circular) (Six-base) MAPSORT of: pFN21AA1230.seq Check: 9580 from: 1 to: 7738
pFN21AA1230 7738 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5548 278
Size: 53 83 186 936 4012 2468
Fragments arranged by size:
4012 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4369 5081 1937
Size: 2432 712 4594
Fragments arranged by size:
4594 2432 712
Acc65I G'GTAC_C
Cuts at: 5059 5059
Size: 7738
AclI AA'CG_TT
Cuts at: 1563 5034 5865 6238 1563
Size: 3471 831 373 3063
Fragments arranged by size:
3471 3063 831 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 2342 2654 2810 2859 3434 3444 4155
Size: 614 96 312 156 49 575 10 711
Cuts at: 4155 4307 4953 5801 7185 7453 1632
Size: 152 646 848 1384 268 1917
Fragments arranged by size:
1917 1384 848 711 646 614 575 312
268 156 152 96 49 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7541
AflIII A'CryG_T
Cuts at: 1203 2025 3828 4956 4974 6637 7614 1203
Size: 822 1803 1128 18 1663 977 1327
Fragments arranged by size:
1803 1663 1327 1128 977 822 18
AgeI A'CCGG_T
Cuts at: 4875 6569 4875
Size: 1694 6044
AhdI GACnn_n'nnGTC
Cuts at: 4279 6467 4279
Size: 2188 5550
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7738
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4035 1184
Size: 2851 4887
AlwNI CAG_nnn'CTG
Cuts at: 3001 3251 4339 4694 5481 7053 3001
Size: 250 1088 355 787 1572 3686
Fragments arranged by size:
3686 1572 1088 787 355 250
ApaI G_GGCC'C
Cuts at: 3879 3879
Size: 7738
ApaLI G'TGCA_C
Cuts at: 3624 5795 6951 3624
Size: 2171 1156 4411
Fragments arranged by size:
4411 2171 1156
ApoI r'AATT_y
Cuts at: 1493 3842 4000 4738 5047 5273 5425 7534 1493
Size: 2349 158 738 309 226 152 2109 1697
Fragments arranged by size:
2349 2109 1697 738 309 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 6292 160
Size: 872 5260 1606
Fragments arranged by size:
5260 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7738
AvaI C'yCGr_G
Cuts at: 1870 1943 3894 5063 1870
Size: 73 1951 1169 4545
Fragments arranged by size:
4545 1951 1169 73
BamHI G'GATC_C
Cuts at: 5068 5435 5068
Size: 367 7371
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3338 5059 6514 618
Size: 325 217 627 1551 1721 1455 1842
Fragments arranged by size:
1842 1721 1551 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2149 3475 3879 3992 4515 5057 1391
Size: 758 1326 404 113 523 542 4072
Fragments arranged by size:
4072 1326 758 542 523 404 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2169 3461 4600 5031 928
Size: 971 270 1292 1139 431 3635
Fragments arranged by size:
3635 1292 1139 971 431 270
BbvCI CC'TCA_GC
Cuts at: 4632 4632
Size: 7738
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5929 5963 1903
Size: 34 3992 34 3678
Fragments arranged by size:
3992 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5630 6840 5630
Size: 1210 6528
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7738
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3520 6349 136
Size: 107 122 71 631 605 1848 2829 1525
Fragments arranged by size:
2829 1848 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2986 3569 3670 4057 7733 2986
Size: 583 101 387 3676 2991
Fragments arranged by size:
3676 2991 583 387 101
BlpI GC'TnA_GC
Cuts at: 5155 5155
Size: 7738
Bme1580I G_kGCm'C
Cuts at: 1790 3628 3879 5799 6955 1790
Size: 1838 251 1920 1156 2573
Fragments arranged by size:
2573 1920 1838 1156 251
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7738
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3159 4180 4213
Size: 416 53 83 399 24 1742 1021 33
Cuts at: 4213 4998 5469 6427 442
Size: 785 471 958 1753
Fragments arranged by size:
1753 1742 1021 958 785 471 416 399
83 53 33 24
BmtI G_CTAG'C
Cuts at: 1056 4115 1056
Size: 3059 4679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3132 3146 3726 4091 6398 1239
Size: 1893 14 580 365 2307 2579
Fragments arranged by size:
2579 2307 1893 580 365 14
Bpu10I CC'TnA_GC
Cuts at: 2391 3291 4632 2391
Size: 900 1341 5497
Fragments arranged by size:
5497 1341 900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2233 5863 6728 7026 2233
Size: 3630 865 298 2945
Fragments arranged by size:
3630 2945 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3897 6401 882
Size: 617 2398 2504 2219
Fragments arranged by size:
2504 2398 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5529 493
Size: 711 4325 2702
Fragments arranged by size:
4325 2702 711
BsaBI GATnn'nnATC
Cuts at: 2307 7732 2307
Size: 5425 2313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5545 5927 6562 275
Size: 53 83 186 936 4012 382 635 1451
Fragments arranged by size:
4012 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4875 6171 6569 6843 6990 7401 4875
Size: 1296 398 274 147 411 5212
Fragments arranged by size:
5212 1296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2874 2904 3490 3520 1178
Size: 30 165 30 1471 30 586 30 5396
Fragments arranged by size:
5396 1471 586 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3082 3867 1186
Size: 1896 785 5057
Fragments arranged by size:
5057 1896 785
BseYI C'CCAG_C
Cuts at: 6941 7437 6941
Size: 496 7242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 1163
Size: 573 399 6766
Fragments arranged by size:
6766 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5188 5949 6977 1540
Size: 275 186 3187 761 1028 2301
Fragments arranged by size:
3187 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2149 2436 3475 3628 3666 5799 5884 6955 2149
Size: 287 1039 153 38 2133 85 1071 2932
Fragments arranged by size:
2932 2133 1071 1039 287 153 85 38
BsmI GAATG_Cn'
Cuts at: 2455 4084 4254 4925 5249 5342 2455
Size: 1629 170 671 324 93 4851
Fragments arranged by size:
4851 1629 671 324 170 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2149 2436 3475 3628 3666 3879 3992
Size: 399 359 287 1039 153 38 213 113
Cuts at: 3992 4515 5057 5799 5884 6955 1391
Size: 523 542 742 85 1071 2174
Fragments arranged by size:
2174 1071 1039 742 542 523 399 359
287 213 153 113 85 38
BspEI T'CCGG_A
Cuts at: 7401 7401
Size: 7738
BspHI T'CATG_A
Cuts at: 3771 5627 7357 3771
Size: 1856 1730 4152
Fragments arranged by size:
4152 1856 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 2175 3946 5093 844
Size: 1213 118 1771 1147 3489
Fragments arranged by size:
3489 1771 1213 1147 118
BsrBI CCG'CTC
Cuts at: 1394 1823 5625 1394
Size: 429 3802 3507
Fragments arranged by size:
3802 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6233 6407 65
Size: 1183 4985 174 1396
Fragments arranged by size:
4985 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4875 6382 6569 1700
Size: 110 3065 1507 187 2869
Fragments arranged by size:
3065 2869 1507 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7738
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7738
BssSI C'ACGA_G
Cuts at: 5798 6810 5798
Size: 1012 6726
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4475 794
Size: 3681 4057
BstBI TT'CG_AA
Cuts at: 6542 6542
Size: 7738
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7738
BstXI CCAn_nnnn'nTGG
Cuts at: 3403 3403
Size: 7738
BstYI r'GATC_y
Cuts at: 1972 2223 2515 2986 3158 3569 3670 4057 4130
Size: 251 292 471 172 411 101 387 73
Cuts at: 4130 5068 5435 5820 5837 7289 7375 7387 7733
Size: 938 367 385 17 1452 86 12 346
Cuts at: 7733 1972
Size: 1977
Fragments arranged by size:
1977 1452 938 471 411 387 385 367
346 292 251 172 101 86 73 17
12
Bsu36I CC'TnA_GG
Cuts at: 4340 4340
Size: 7738
BtgI C'CryG_G
Cuts at: 513 1065 3534 3870 4458 4504 4659 513
Size: 552 2469 336 588 46 155 3592
Fragments arranged by size:
3592 2469 588 552 336 155 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4384 487
Size: 683 56 3158 3841
Fragments arranged by size:
3841 3158 683 56
BtsI GCAGTG_nn'
Cuts at: 4501 5254 6048 6068 4501
Size: 753 794 20 6171
Fragments arranged by size:
6171 794 753 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7738
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7703
DraI TTT'AAA
Cuts at: 2811 3595 4831 4981 5042 2811
Size: 784 1236 150 61 5507
Fragments arranged by size:
5507 1236 784 150 61
DraIII CAC_nnn'GTG
Cuts at: 2158 2158
Size: 7738
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6745 809
Size: 507 5429 1802
Fragments arranged by size:
5429 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2709 3580 3692
Size: 54 1425 50 275 18 879 871 112
Cuts at: 3692 4027 4379 4602 5185 6074 7703 8
Size: 335 352 223 583 889 1629 43
Fragments arranged by size:
1629 1425 889 879 871 583 352 335
275 223 112 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5185 1537
Size: 275 3373 4090
Fragments arranged by size:
4090 3373 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2424 3060 3192 5668 1353
Size: 1071 636 132 2476 3423
Fragments arranged by size:
3423 2476 1071 636 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6325 6699 6845 387
Size: 1064 138 4736 374 146 1280
Fragments arranged by size:
4736 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2147 3473 2147
Size: 1326 6412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2342 2654 2810 2859 3132 3146
Size: 393 614 96 312 156 49 273 14
Cuts at: 3146 3434 3444 3726 4091 4155 4307 4953 5801
Size: 288 10 282 365 64 152 646 848
Cuts at: 5801 6398 7185 7453 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 848 787 646 614 597 393 365
312 288 282 273 268 156 152 96
64 49 14 10
EcoNI CCTnn'n_nnAGG
Cuts at: 4856 4856
Size: 7738
EcoO109I rG'GnC_Cy
Cuts at: 3498 3513 3875 3876 4336 3498
Size: 15 362 1 460 6900
Fragments arranged by size:
6900 460 362 15 1
EcoRI G'AATT_C
Cuts at: 3842 4000 5047 5425 3842
Size: 158 1047 378 6155
Fragments arranged by size:
6155 1047 378 158
FspI TGC'GCA
Cuts at: 6244 6244
Size: 7738
HaeII r_GCGC'y
Cuts at: 1678 6885 1678
Size: 5207 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2208 2240 2330
Size: 32 135 32 524 32 80 32 90
Cuts at: 2330 2362 3490 3522 3700 3718 3732 3750 3815
Size: 32 1128 32 178 18 14 18 65
Cuts at: 3815 3847 4265 4297 4417 4449 5012 5044 6379
Size: 32 418 32 120 32 563 32 1335
Cuts at: 6379 6411 6453 6485 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1335 1128 563 524 418 178 135
120 90 80 65 42 32 32 32
32 32 32 32 32 32 32 32
32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2373 3285 5082 5328 669
Size: 988 281 435 912 1797 246 3079
Fragments arranged by size:
3079 1797 988 912 435 281 246
HindIII A'AGCT_T
Cuts at: 748 2684 748
Size: 1936 5802
HpaI GTT'AAC
Cuts at: 2373 5328 2373
Size: 2955 4783
KpnI G_GTAC'C
Cuts at: 5063 5063
Size: 7738
MfeI C'AATT_G
Cuts at: 3964 5337 3964
Size: 1373 6365
MluI A'CGCG_T
Cuts at: 7614 7614
Size: 7738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2022 2493 4167 6852 7036 1305
Size: 717 471 1674 2685 184 2007
Fragments arranged by size:
2685 2007 1674 717 471 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2711 4029 4604 10
Size: 54 1425 343 879 1318 575 3144
Fragments arranged by size:
3144 1425 1318 879 575 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3533 5696 6055 6214 518
Size: 648 2367 2163 359 159 2042
Fragments arranged by size:
2367 2163 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2558 2764 3872 5154 5831 6979 7224 1606
Size: 952 206 1108 1282 677 1148 245 2120
Fragments arranged by size:
2120 1282 1148 1108 952 677 245 206
NcoI C'CATG_G
Cuts at: 513 1065 3534 4504 4659 513
Size: 552 2469 970 155 3592
Fragments arranged by size:
3592 2469 970 552 155
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7738
NheI G'CTAG_C
Cuts at: 1052 4111 1052
Size: 3059 4679
NotI GC'GGCC_GC
Cuts at: 5185 5185
Size: 7738
NspI r_CATG'y
Cuts at: 1132 1229 2029 3832 4960 4978 5096 6641 1132
Size: 97 800 1803 1128 18 118 1545 2229
Fragments arranged by size:
2229 1803 1545 1128 800 118 97 18
PasI CC'CwG_GG
Cuts at: 1348 3518 4126 1348
Size: 2170 608 4960
Fragments arranged by size:
4960 2170 608
PciI A'CATG_T
Cuts at: 2025 3828 4956 4974 6637 2025
Size: 1803 1128 18 1663 3126
Fragments arranged by size:
3126 1803 1663 1128 18
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4126 1108
Size: 3018 4720
PmeI GTTT'AAAC
Cuts at: 5042 5042
Size: 7738
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5807 5839 7320 7352 5807
Size: 32 1481 32 6193
Fragments arranged by size:
6193 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3498 4336 3498
Size: 838 6900
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7738
PsiI TTA'TAA
Cuts at: 5308 5308
Size: 7738
PspOMI G'GGCC_C
Cuts at: 3875 3875
Size: 7738
PstI C_TGCA'G
Cuts at: 830 2038 2472 3390 5090 830
Size: 1208 434 918 1700 3478
Fragments arranged by size:
3478 1700 1208 918 434
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7738
PvuII CAG'CTG
Cuts at: 2558 2558
Size: 7738
SacI G_AGCT'C
Cuts at: 2149 3475 2149
Size: 1326 6412
SacII CC_GC'GG
Cuts at: 3873 3873
Size: 7738
SalI G'TCGA_C
Cuts at: 1936 5080 1936
Size: 3144 4594
SanDI GG'GwC_CC
Cuts at: 3498 3498
Size: 7738
SapI GCTCTTCn'nnn_
Cuts at: 2424 3060 2424
Size: 636 7102
SbfI CC_TGCA'GG
Cuts at: 5090 5090
Size: 7738
ScaI AGT'ACT
Cuts at: 5986 7698 5986
Size: 1712 6026
SexAI A'CCwGG_T
Cuts at: 2379 4834 2379
Size: 2455 5283
SfcI C'TryA_G
Cuts at: 826 1045 2034 2468 2559 3386 5086 6221 6902
Size: 219 989 434 91 827 1700 1135 681
Cuts at: 6902 7093 826
Size: 191 1471
Fragments arranged by size:
1700 1471 1135 989 827 681 434 219
191 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7738
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7738
SmaI CCC'GGG
Cuts at: 1872 5065 1872
Size: 3193 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2212 3894 5842 6743 7005 820
Size: 197 926 269 1682 1948 901 262 1553
Fragments arranged by size:
1948 1682 1553 926 901 269 262 197
SnaBI TAC'GTA
Cuts at: 493 5529 493
Size: 5036 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7738
SphI G_CATG'C
Cuts at: 1132 5096 1132
Size: 3964 3774
SspI AAT'ATT
Cuts at: 5 52 2087 5662 5
Size: 47 2035 3575 2081
Fragments arranged by size:
3575 2081 2035 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7738
StyI C'CwwG_G
Cuts at: 513 1065 3043 3334 3534 3948 4504 4659 5177
Size: 552 1978 291 200 414 556 155 518
Cuts at: 5177 513
Size: 3074
Fragments arranged by size:
3074 1978 556 552 518 414 291 200
155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3157 4588 5765 5782 5935 6120 6565
Size: 1255 1577 1431 1177 17 153 185 445
Cuts at: 6565 325
Size: 1498
Fragments arranged by size:
1577 1498 1431 1255 1177 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2917 4678 5984
Size: 274 80 33 51 1443 940 1761 1306
Cuts at: 5984 7696 96
Size: 1712 138
Fragments arranged by size:
1761 1712 1443 1306 940 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3175 3207 7698 7730 3175
Size: 32 4491 32 3183
Fragments arranged by size:
4491 3183 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7738
XbaI T'CTAG_A
Cuts at: 4060 4522 5074 4060
Size: 462 552 6724
Fragments arranged by size:
6724 552 462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2704 3050 3151 3939 2704
Size: 346 101 788 6503
Fragments arranged by size:
6503 788 346 101
XhoI C'TCGA_G
Cuts at: 1943 3894 1943
Size: 1951 5787
XmaI C'CCGG_G
Cuts at: 1870 5063 1870
Size: 3193 4545
XmnI GAAnn'nnTTC
Cuts at: 2259 2988 3447 5867 2259
Size: 729 459 2420 4130
Fragments arranged by size:
4130 2420 729 459
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5546 276
Size: 53 83 186 936 4012 2468
Fragments arranged by size:
4012 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SfiI SfoI SrfI SwaI