Restriction map for
pFN21AA1221
(Circular) (Six-base) MAPSORT of: pFN21AA1221.seq Check: 1170 from: 1 to: 8686
pFN21AA1221 8686 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3208 3208
Size: 8686
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6496 278
Size: 53 83 186 936 4960 2468
Fragments arranged by size:
4960 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2495 2973 3009 6029 1937
Size: 558 478 36 3020 4594
Fragments arranged by size:
4594 3020 558 478 36
Acc65I G'GTAC_C
Cuts at: 3515 6007 3515
Size: 2492 6194
AclI AA'CG_TT
Cuts at: 1563 6813 7186 1563
Size: 5250 373 3063
Fragments arranged by size:
5250 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 2471 2583 3449 3774 5427 5967 6749
Size: 618 221 112 866 325 1653 540 782
Cuts at: 6749 8133 8401 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1653 1384 866 782 618 540 325
268 221 112
AflII C'TTAA_G
Cuts at: 820 1017 2263 820
Size: 197 1246 7243
Fragments arranged by size:
7243 1246 197
AflIII A'CryG_T
Cuts at: 1203 4095 4417 7585 8562 1203
Size: 2892 322 3168 977 1327
Fragments arranged by size:
3168 2892 1327 977 322
AgeI A'CCGG_T
Cuts at: 7517 7517
Size: 8686
AhdI GACnn_n'nnGTC
Cuts at: 7415 7415
Size: 8686
AleI CACnn'nnGTG
Cuts at: 1166 4416 1166
Size: 3250 5436
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8686
AlwNI CAG_nnn'CTG
Cuts at: 5423 6429 8001 5423
Size: 1006 1572 6108
Fragments arranged by size:
6108 1572 1006
ApaLI G'TGCA_C
Cuts at: 3483 6743 7899 3483
Size: 3260 1156 4270
Fragments arranged by size:
4270 3260 1156
ApoI r'AATT_y
Cuts at: 1493 2620 2733 3256 3444 4032 4312 4666 5446
Size: 1127 113 523 188 588 280 354 780
Cuts at: 5446 5557 5995 6221 6373 8482 1493
Size: 111 438 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 780 588 523 438 354
280 226 188 152 113 111
AseI AT'TA_AT
Cuts at: 160 1032 4650 7240 160
Size: 872 3618 2590 1606
Fragments arranged by size:
3618 2590 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8686
AvaI C'yCGr_G
Cuts at: 1870 1943 6011 1870
Size: 73 4068 4545
Fragments arranged by size:
4545 4068 73
BamHI G'GATC_C
Cuts at: 2782 6016 6383 2782
Size: 3234 367 5085
Fragments arranged by size:
5085 3234 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3515 6007 7462 618
Size: 325 217 627 1728 2492 1455 1842
Fragments arranged by size:
2492 1842 1728 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 6005 1391
Size: 4614 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3066 3430 4482 5844 928
Size: 971 1167 364 1052 1362 3770
Fragments arranged by size:
3770 1362 1167 1052 971 364
BbvCI CC'TCA_GC
Cuts at: 4812 4812
Size: 8686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6877 6911 1903
Size: 34 4940 34 3678
Fragments arranged by size:
4940 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6578 7788 6578
Size: 1210 7476
BclI T'GATC_A
Cuts at: 1548 4251 1548
Size: 2703 5983
BfrBI ATG'CAT
Cuts at: 3026 3026
Size: 8686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3636 7297 136
Size: 107 122 71 631 605 1964 3661 1525
Fragments arranged by size:
3661 1964 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5726 8681 5726
Size: 2955 5731
BlpI GC'TnA_GC
Cuts at: 6103 6103
Size: 8686
Bme1580I G_kGCm'C
Cuts at: 1790 3487 6747 7903 1790
Size: 1697 3260 1156 2573
Fragments arranged by size:
3260 2573 1697 1156
BmgBI CAC'GTC
Cuts at: 1318 4418 5916 1318
Size: 3100 1498 4088
Fragments arranged by size:
4088 3100 1498
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2788 5481 6417
Size: 416 53 83 399 24 1371 2693 936
Cuts at: 6417 7375 442
Size: 958 1753
Fragments arranged by size:
2693 1753 1371 958 936 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4731 5913 7346 1239
Size: 3492 1182 1433 2579
Fragments arranged by size:
3492 2579 1433 1182
Bpu10I CC'TnA_GC
Cuts at: 4812 4812
Size: 8686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4904 6811 7676 7974 4904
Size: 1907 865 298 5616
Fragments arranged by size:
5616 1907 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7349 882
Size: 617 5850 2219
Fragments arranged by size:
5850 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6477 493
Size: 711 5273 2702
Fragments arranged by size:
5273 2702 711
BsaBI GATnn'nnATC
Cuts at: 4488 4650 8680 4488
Size: 162 4030 4494
Fragments arranged by size:
4494 4030 162
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4383 6493 6875 7510
Size: 53 83 186 936 2850 2110 382 635
Cuts at: 7510 275
Size: 1451
Fragments arranged by size:
2850 2110 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7119 7517 7791 7938 8349 7119
Size: 398 274 147 411 7456
Fragments arranged by size:
7456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2334 2364 1178
Size: 30 165 30 931 30 7500
Fragments arranged by size:
7500 931 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2362 4107 5562 1186
Size: 1176 1745 1455 4310
Fragments arranged by size:
4310 1745 1455 1176
BseYI C'CCAG_C
Cuts at: 4909 7889 8385 4909
Size: 2980 496 5210
Fragments arranged by size:
5210 2980 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6136 6897 7925 1540
Size: 275 186 4135 761 1028 2301
Fragments arranged by size:
4135 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2305 3487 6747 6832 7903 2305
Size: 1182 3260 85 1071 3088
Fragments arranged by size:
3260 3088 1182 1071 85
BsmI GAATG_Cn'
Cuts at: 3632 3829 6197 6290 3632
Size: 197 2368 93 6028
Fragments arranged by size:
6028 2368 197 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2305 3487 6005 6747 6832 7903 1391
Size: 399 515 1182 2518 742 85 1071 2174
Fragments arranged by size:
2518 2174 1182 1071 742 515 399 85
BspEI T'CCGG_A
Cuts at: 8349 8349
Size: 8686
BspHI T'CATG_A
Cuts at: 3717 6575 8305 3717
Size: 2858 1730 4098
Fragments arranged by size:
4098 2858 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3208 5784 6041 844
Size: 2364 2576 257 3489
Fragments arranged by size:
3489 2576 2364 257
BsrBI CCG'CTC
Cuts at: 1394 1823 2166 6573 1394
Size: 429 343 4407 3507
Fragments arranged by size:
4407 3507 429 343
BsrDI GCAATG_nn'
Cuts at: 65 1248 3256 3639 4074 7181 7355 65
Size: 1183 2008 383 435 3107 174 1396
Fragments arranged by size:
3107 2008 1396 1183 435 383 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7330 7517 1700
Size: 110 5520 187 2869
Fragments arranged by size:
5520 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3239 96
Size: 3143 5543
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8686
BssSI C'ACGA_G
Cuts at: 6746 7758 6746
Size: 1012 7674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8686
BstBI TT'CG_AA
Cuts at: 7490 7490
Size: 8686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8686
BstXI CCAn_nnnn'nTGG
Cuts at: 2080 4745 2080
Size: 2665 6021
BstYI r'GATC_y
Cuts at: 1972 2782 5580 5726 6016 6383 6768 6785 8237
Size: 810 2798 146 290 367 385 17 1452
Cuts at: 8237 8323 8335 8681 1972
Size: 86 12 346 1977
Fragments arranged by size:
2798 1977 1452 810 385 367 346 290
146 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2740 2740
Size: 8686
BtgI C'CryG_G
Cuts at: 513 1065 5885 513
Size: 552 4820 3314
Fragments arranged by size:
4820 3314 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7947
Fragments arranged by size:
7947 683 56
BtsI GCAGTG_nn'
Cuts at: 2413 3612 3660 6202 6996 7016 2413
Size: 1199 48 2542 794 20 4083
Fragments arranged by size:
4083 2542 1199 794 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8651
DraI TTT'AAA
Cuts at: 3807 3975 5990 3807
Size: 168 2015 6503
Fragments arranged by size:
6503 2015 168
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4876 7693 809
Size: 507 3560 2817 1802
Fragments arranged by size:
3560 2817 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4043 5970 6133
Size: 54 1425 50 275 18 2213 1927 163
Cuts at: 6133 7022 8651 8
Size: 889 1629 43
Fragments arranged by size:
2213 1927 1629 1425 889 275 163 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6133 1537
Size: 275 4321 4090
Fragments arranged by size:
4321 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2988 3114 3174 3213 3582 6616 1353
Size: 1635 126 60 39 369 3034 3423
Fragments arranged by size:
3423 3034 1635 369 126 60 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7273 7647 7793 387
Size: 1064 138 5684 374 146 1280
Fragments arranged by size:
5684 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2471 2583 3449 3774 4731 5427
Size: 393 618 221 112 866 325 957 696
Cuts at: 5427 5913 5967 6749 7346 8133 8401 1239
Size: 486 54 782 597 787 268 1524
Fragments arranged by size:
1524 957 866 787 782 696 618 597
486 393 325 268 221 112 54
EcoO109I rG'GnC_Cy
Cuts at: 5819 5819
Size: 8686
EcoRI G'AATT_C
Cuts at: 2620 5557 5995 6373 2620
Size: 2937 438 378 4933
Fragments arranged by size:
4933 2937 438 378
FspI TGC'GCA
Cuts at: 7192 7192
Size: 8686
HaeII r_GCGC'y
Cuts at: 1678 7833 1678
Size: 6155 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3053 3085 3179 3211 4792
Size: 32 135 32 1481 32 94 32 1581
Cuts at: 4792 4824 4877 4909 5342 5374 5395 5427 5572
Size: 32 53 32 433 32 21 32 145
Cuts at: 5572 5604 7327 7359 7401 7433 1373
Size: 32 1723 32 42 32 2626
Fragments arranged by size:
2626 1723 1581 1481 433 145 135 94
53 42 32 32 32 32 32 32
32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2220 2516 3948 4150 6030 6276
Size: 988 281 282 296 1432 202 1880 246
Cuts at: 6276 669
Size: 3079
Fragments arranged by size:
3079 1880 1432 988 296 282 281 246
202
HindIII A'AGCT_T
Cuts at: 748 4399 748
Size: 3651 5035
HpaI GTT'AAC
Cuts at: 6276 6276
Size: 8686
KpnI G_GTAC'C
Cuts at: 3519 6011 3519
Size: 2492 6194
MfeI C'AATT_G
Cuts at: 3520 6285 3520
Size: 2765 5921
MluI A'CGCG_T
Cuts at: 8562 8562
Size: 8686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3109 4447 7800 7984 1305
Size: 1804 1338 3353 184 2007
Fragments arranged by size:
3353 2007 1804 1338 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6864
Fragments arranged by size:
6864 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 3303 3330 3605 3838 4125 4233
Size: 648 1132 1005 27 275 233 287 108
Cuts at: 4233 4416 6644 7003 7162 518
Size: 183 2228 359 159 2042
Fragments arranged by size:
2228 2042 1132 1005 648 359 287 275
233 183 159 108 27
MspA1I CmG'CkG
Cuts at: 1606 2480 2491 6102 6779 7927 8172 1606
Size: 874 11 3611 677 1148 245 2120
Fragments arranged by size:
3611 2120 1148 874 677 245 11
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8134
NdeI CA'TA_TG
Cuts at: 387 3288 3346 3402 3764 387
Size: 2901 58 56 362 5309
Fragments arranged by size:
5309 2901 362 58 56
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8686
NotI GC'GGCC_GC
Cuts at: 6133 6133
Size: 8686
NsiI A_TGCA'T
Cuts at: 3028 3028
Size: 8686
NspI r_CATG'y
Cuts at: 1132 1229 2297 2555 3548 4099 4664 6044 7589
Size: 97 1068 258 993 551 565 1380 1545
Cuts at: 7589 1132
Size: 2229
Fragments arranged by size:
2229 1545 1380 1068 993 565 551 258
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8686
PciI A'CATG_T
Cuts at: 4095 7585 4095
Size: 3490 5196
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5891 1108
Size: 4783 3903
PmeI GTTT'AAAC
Cuts at: 5990 5990
Size: 8686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6755 6787 8268 8300 6755
Size: 32 1481 32 7141
Fragments arranged by size:
7141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5819 5819
Size: 8686
PshAI GACnn'nnGTC
Cuts at: 1613 4517 5824 1613
Size: 2904 1307 4475
Fragments arranged by size:
4475 2904 1307
PsiI TTA'TAA
Cuts at: 3099 6256 3099
Size: 3157 5529
PstI C_TGCA'G
Cuts at: 830 4831 6038 830
Size: 4001 1207 3478
Fragments arranged by size:
4001 3478 1207
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8686
PvuII CAG'CTG
Cuts at: 2480 2491 2480
Size: 11 8675
SalI G'TCGA_C
Cuts at: 1936 6028 1936
Size: 4092 4594
SapI GCTCTTCn'nnn_
Cuts at: 2988 3213 2988
Size: 225 8461
SbfI CC_TGCA'GG
Cuts at: 6038 6038
Size: 8686
ScaI AGT'ACT
Cuts at: 3824 6934 8646 3824
Size: 3110 1712 3864
Fragments arranged by size:
3864 3110 1712
SfcI C'TryA_G
Cuts at: 826 1045 3729 3906 4243 4639 4827 5088 6034
Size: 219 2684 177 337 396 188 261 946
Cuts at: 6034 7169 7850 8041 826
Size: 1135 681 191 1471
Fragments arranged by size:
2684 1471 1135 946 681 396 337 261
219 191 188 177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8686
SmaI CCC'GGG
Cuts at: 1872 6013 1872
Size: 4141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2263 4883 6790 7691 7953 820
Size: 197 926 320 2620 1907 901 262 1553
Fragments arranged by size:
2620 1907 1553 926 901 320 262 197
SnaBI TAC'GTA
Cuts at: 493 6477 493
Size: 5984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8686
SphI G_CATG'C
Cuts at: 1132 6044 1132
Size: 4912 3774
SspI AAT'ATT
Cuts at: 5 52 6610 5
Size: 47 6558 2081
Fragments arranged by size:
6558 2081 47
StuI AGG'CCT
Cuts at: 1517 4393 1517
Size: 2876 5810
StyI C'CwwG_G
Cuts at: 513 1065 3338 4200 6125 513
Size: 552 2273 862 1925 3074
Fragments arranged by size:
3074 2273 1925 862 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2582 2666 6713 6730 6883 7068 7513
Size: 1255 1002 84 4047 17 153 185 445
Cuts at: 7513 325
Size: 1498
Fragments arranged by size:
4047 1498 1255 1002 445 185 153 84
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3239 3822 6932
Size: 274 80 33 51 1443 1262 583 3110
Cuts at: 6932 8644 96
Size: 1712 138
Fragments arranged by size:
3110 1712 1443 1262 583 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2418 2450 8646 8678 2418
Size: 32 6196 32 2426
Fragments arranged by size:
6196 2426 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8686
XbaI T'CTAG_A
Cuts at: 2045 2863 2980 6022 2045
Size: 818 117 3042 4709
Fragments arranged by size:
4709 3042 818 117
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8686
XmaI C'CCGG_G
Cuts at: 1870 6011 1870
Size: 4141 4545
XmnI GAAnn'nnTTC
Cuts at: 6815 6815
Size: 8686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6494 276
Size: 53 83 186 936 4960 2468
Fragments arranged by size:
4960 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI