Restriction map for
pFN21AA1216
(Circular) (Six-base) MAPSORT of: pFN21AA1216.seq Check: 711 from: 1 to: 8809
pFN21AA1216 8809 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5540 6619 278
Size: 53 83 186 936 4004 1079 2468
Fragments arranged by size:
4004 2468 1079 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5748 6152 1937
Size: 3811 404 4594
Fragments arranged by size:
4594 3811 404
Acc65I G'GTAC_C
Cuts at: 6130 6130
Size: 8809
AclI AA'CG_TT
Cuts at: 1563 6936 7309 1563
Size: 5373 373 3063
Fragments arranged by size:
5373 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 2119 2601 2623 3537 4065 4136 5126
Size: 483 4 482 22 914 528 71 990
Cuts at: 5126 5721 6872 8256 8524 1632
Size: 595 1151 1384 268 1917
Fragments arranged by size:
1917 1384 1151 990 914 595 528 483
482 268 71 22 4
AfeI AGC'GCT
Cuts at: 2891 2891
Size: 8809
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8612
AflIII A'CryG_T
Cuts at: 1203 7708 8685 1203
Size: 6505 977 1327
Fragments arranged by size:
6505 1327 977
AgeI A'CCGG_T
Cuts at: 7640 7640
Size: 8809
AhdI GACnn_n'nnGTC
Cuts at: 7538 7538
Size: 8809
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8809
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2166 2198 2166
Size: 32 8777
AlwNI CAG_nnn'CTG
Cuts at: 2485 2558 3239 3874 4043 4115 4669 6552 8124
Size: 73 681 635 169 72 554 1883 1572
Cuts at: 8124 2485
Size: 3170
Fragments arranged by size:
3170 1883 1572 681 635 554 169 73
72
ApaLI G'TGCA_C
Cuts at: 6866 8022 6866
Size: 1156 7653
ApoI r'AATT_y
Cuts at: 1493 2229 3454 4548 4723 4991 5729 6118 6344
Size: 736 1225 1094 175 268 738 389 226
Cuts at: 6344 6496 8605 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1225 1094 738 736 389 268
226 175 152
AseI AT'TA_AT
Cuts at: 160 1032 4413 4598 7363 160
Size: 872 3381 185 2765 1606
Fragments arranged by size:
3381 2765 1606 872 185
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8809
AvaI C'yCGr_G
Cuts at: 1870 1943 6134 1870
Size: 73 4191 4545
Fragments arranged by size:
4545 4191 73
BamHI G'GATC_C
Cuts at: 2734 4828 5878 6139 6506 2734
Size: 2094 1050 261 367 5037
Fragments arranged by size:
5037 2094 1050 367 261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2708 5652 6082 6130 7585
Size: 325 217 627 921 2944 430 48 1455
Cuts at: 7585 618
Size: 1842
Fragments arranged by size:
2944 1842 1455 921 627 430 325 217
48
BanII G_rGCy'C
Cuts at: 1391 2465 3487 4358 5340 6128 1391
Size: 1074 1022 871 982 788 4072
Fragments arranged by size:
4072 1074 1022 982 871 788
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2675 4370 4877 5729 5981 928
Size: 971 776 1695 507 852 252 3756
Fragments arranged by size:
3756 1695 971 852 776 507 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3929 3963 5212 5246 7000 7034 1903
Size: 34 1992 34 1249 34 1754 34 3678
Fragments arranged by size:
3678 1992 1754 1249 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2080 2192 2586 2626 3516 4954 6701 7911 2080
Size: 112 394 40 890 1438 1747 1210 2978
Fragments arranged by size:
2978 1747 1438 1210 890 394 112 40
BclI T'GATC_A
Cuts at: 1548 5457 1548
Size: 3909 4900
BfrBI ATG'CAT
Cuts at: 3443 3794 3841 4542 3443
Size: 351 47 701 7710
Fragments arranged by size:
7710 701 351 47
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7420 136
Size: 107 122 71 631 605 5748 1525
Fragments arranged by size:
5748 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2410 8804 2410
Size: 6394 2415
BlpI GC'TnA_GC
Cuts at: 2251 6226 2251
Size: 3975 4834
Bme1580I G_kGCm'C
Cuts at: 1790 6087 6870 8026 1790
Size: 4297 783 1156 2573
Fragments arranged by size:
4297 2573 1156 783
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3227 3250 6540
Size: 416 53 83 399 24 1810 23 3290
Cuts at: 6540 7498 442
Size: 958 1753
Fragments arranged by size:
3290 1810 1753 958 416 399 83 53
24 23
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8809
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3543 3887 3966 4188 3543
Size: 344 79 222 8164
Fragments arranged by size:
8164 344 222 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2924 3669 4976 5299 6045 7469 1239
Size: 1685 745 1307 323 746 1424 2579
Fragments arranged by size:
2579 1685 1424 1307 746 745 323
Bpu10I CC'TnA_GC
Cuts at: 4199 4199
Size: 8809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 2992 3839 6934 7799 8097 2345
Size: 647 847 3095 865 298 3057
Fragments arranged by size:
3095 3057 865 847 647 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2279 2945 5049 6063 7472 882
Size: 617 780 666 2104 1014 1409 2219
Fragments arranged by size:
2219 2104 1409 1014 780 666 617
BsaAI yAC'GTr
Cuts at: 493 1204 6600 493
Size: 711 5396 2702
Fragments arranged by size:
5396 2702 711
BsaBI GATnn'nnATC
Cuts at: 2784 8803 2784
Size: 6019 2790
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5537 6616 6998 7633
Size: 53 83 186 936 4004 1079 382 635
Cuts at: 7633 275
Size: 1451
Fragments arranged by size:
4004 1451 1079 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3834 4939 7242 7640 7914 8061 8472 3834
Size: 1105 2303 398 274 147 411 4171
Fragments arranged by size:
4171 2303 1105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2897 2927 3474 3504 3798
Size: 30 165 30 1494 30 547 30 294
Cuts at: 3798 3828 4277 4307 1178
Size: 30 449 30 5680
Fragments arranged by size:
5680 1494 547 449 294 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2972 4164 4301 4304 4353 1186
Size: 1786 1192 137 3 49 5642
Fragments arranged by size:
5642 1786 1192 137 49 3
BseYI C'CCAG_C
Cuts at: 5302 5528 8012 8508 5302
Size: 226 2484 496 5603
Fragments arranged by size:
5603 2484 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5015 1163
Size: 573 3279 4957
Fragments arranged by size:
4957 3279 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5120 6259 7020 8048 1540
Size: 275 186 3119 1139 761 1028 2301
Fragments arranged by size:
3119 2301 1139 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2465 3487 3947 4032 4737 5340 6870 6955 8026
Size: 1022 460 85 705 603 1530 85 1071
Cuts at: 8026 2465
Size: 3248
Fragments arranged by size:
3248 1530 1071 1022 705 603 460 85
85
BsmI GAATG_Cn'
Cuts at: 4226 5170 5380 5449 6320 6413 4226
Size: 944 210 69 871 93 6622
Fragments arranged by size:
6622 944 871 210 93 69
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2003
Size: 8809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2465 3487 3947 4032 4358 4737 5340
Size: 399 675 1022 460 85 326 379 603
Cuts at: 5340 6087 6128 6870 6955 8026 1391
Size: 747 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 1022 747 742 675 603 460
399 379 326 85 85 41
BspEI T'CCGG_A
Cuts at: 8472 8472
Size: 8809
BspHI T'CATG_A
Cuts at: 2170 4161 4996 5497 6698 8428 2170
Size: 1991 835 501 1201 1730 2551
Fragments arranged by size:
2551 1991 1730 1201 835 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6164 844
Size: 5320 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6696 1394
Size: 429 4873 3507
Fragments arranged by size:
4873 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2262 3282 3630 7304 7478 65
Size: 1183 1014 1020 348 3674 174 1396
Fragments arranged by size:
3674 1396 1183 1020 1014 348 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7453 7640 1700
Size: 110 5643 187 2869
Fragments arranged by size:
5643 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2473 4837 96
Size: 2377 2364 4068
Fragments arranged by size:
4068 2377 2364
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8809
BssSI C'ACGA_G
Cuts at: 5169 6869 7881 5169
Size: 1700 1012 6097
Fragments arranged by size:
6097 1700 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8809
BstBI TT'CG_AA
Cuts at: 2031 7613 2031
Size: 5582 3227
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8809
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 4459 4737 4954 5759 2848
Size: 1611 278 217 805 5898
Fragments arranged by size:
5898 1611 805 278 217
BstYI r'GATC_y
Cuts at: 1972 2410 2734 2785 2920 3557 3801 4828 4842
Size: 438 324 51 135 637 244 1027 14
Cuts at: 4842 5878 6139 6506 6891 6908 8360 8446 8458
Size: 1036 261 367 385 17 1452 86 12
Cuts at: 8458 8804 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1036 1027 637 438 385 367
346 324 261 244 135 86 51 17
14 12
BstZ17I GTA'TAC
Cuts at: 5749 5749
Size: 8809
Bsu36I CC'TnA_GG
Cuts at: 4251 4251
Size: 8809
BtgI C'CryG_G
Cuts at: 513 1065 2004 2937 3477 513
Size: 552 939 933 540 5845
Fragments arranged by size:
5845 939 933 552 540
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8070
Fragments arranged by size:
8070 683 56
BtsI GCAGTG_nn'
Cuts at: 3945 5463 5583 6036 6325 7119 7139 3945
Size: 1518 120 453 289 794 20 5615
Fragments arranged by size:
5615 1518 794 453 289 120 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2975 3010 529
Size: 35 2411 35 6328
Fragments arranged by size:
6328 2411 35 35
DraI TTT'AAA
Cuts at: 2362 2871 5868 5979 6113 2362
Size: 509 2997 111 134 5058
Fragments arranged by size:
5058 2997 509 134 111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7816 809
Size: 507 6500 1802
Fragments arranged by size:
6500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4611 6256 7145
Size: 54 1425 50 275 18 2781 1645 889
Cuts at: 7145 8774 8
Size: 1629 43
Fragments arranged by size:
2781 1645 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6256 1537
Size: 275 4444 4090
Fragments arranged by size:
4444 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 2055 2751 3075 3203 6739 1353
Size: 675 27 696 324 128 3536 3423
Fragments arranged by size:
3536 3423 696 675 324 128 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5558 7396 7770 7916 387
Size: 1064 138 3969 1838 374 146 1280
Fragments arranged by size:
3969 1838 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2463 3485 5338 2463
Size: 1022 1853 5934
Fragments arranged by size:
5934 1853 1022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2119 2601 2623 2924 3537 3669
Size: 393 483 4 482 22 301 613 132
Cuts at: 3669 4065 4136 4976 5126 5299 5721 6045 6872
Size: 396 71 840 150 173 422 324 827
Cuts at: 6872 7469 8256 8524 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 840 827 787 613 597 483 482
422 396 393 324 301 268 173 150
132 71 22 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2760 2760
Size: 8809
EcoO109I rG'GnC_Cy
Cuts at: 2130 4455 4810 2130
Size: 2325 355 6129
Fragments arranged by size:
6129 2325 355
EcoRI G'AATT_C
Cuts at: 5729 6118 6496 5729
Size: 389 378 8042
Fragments arranged by size:
8042 389 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2250 2606 4166 5160 5424 5726 2250
Size: 356 1560 994 264 302 5333
Fragments arranged by size:
5333 1560 994 356 302 264
FspI TGC'GCA
Cuts at: 7315 7315
Size: 8809
HaeII r_GCGC'y
Cuts at: 1678 2893 7956 1678
Size: 1215 5063 2531
Fragments arranged by size:
5063 2531 1215
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4587 4619 4834 4866 7450
Size: 32 135 32 3015 32 215 32 2584
Cuts at: 7450 7482 7524 7556 1373
Size: 32 42 32 2626
Fragments arranged by size:
3015 2626 2584 215 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 5848 6153 6399 669
Size: 988 281 1323 2587 305 246 3079
Fragments arranged by size:
3079 2587 1323 988 305 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8809
HpaI GTT'AAC
Cuts at: 6399 6399
Size: 8809
KpnI G_GTAC'C
Cuts at: 6134 6134
Size: 8809
MfeI C'AATT_G
Cuts at: 5448 6408 5448
Size: 960 7849
MluI A'CGCG_T
Cuts at: 8685 8685
Size: 8809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5740 7923 8107 1305
Size: 4435 2183 184 2007
Fragments arranged by size:
4435 2183 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4613 10
Size: 54 1425 343 2781 4206
Fragments arranged by size:
4206 2781 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 3793 3933 5037 6767 7126 7285
Size: 648 915 1712 140 1104 1730 359 159
Cuts at: 7285 518
Size: 2042
Fragments arranged by size:
2042 1730 1712 1104 915 648 359 159
140
MspA1I CmG'CkG
Cuts at: 1606 5306 6026 6225 6902 8050 8295 1606
Size: 3700 720 199 677 1148 245 2120
Fragments arranged by size:
3700 2120 1148 720 677 245 199
NcoI C'CATG_G
Cuts at: 513 1065 2004 2937 3477 513
Size: 552 939 933 540 5845
Fragments arranged by size:
5845 939 933 552 540
NdeI CA'TA_TG
Cuts at: 387 2776 3057 387
Size: 2389 281 6139
Fragments arranged by size:
6139 2389 281
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8809
NotI GC'GGCC_GC
Cuts at: 6256 6256
Size: 8809
NsiI A_TGCA'T
Cuts at: 3445 3796 3843 4544 3445
Size: 351 47 701 7710
Fragments arranged by size:
7710 701 351 47
NspI r_CATG'y
Cuts at: 1132 1229 2080 3794 5645 5836 6167 7712 1132
Size: 97 851 1714 1851 191 331 1545 2229
Fragments arranged by size:
2229 1851 1714 1545 851 331 191 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8809
PciI A'CATG_T
Cuts at: 7708 7708
Size: 8809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2917 1108
Size: 1809 7000
PfoI T'CCnGG_A
Cuts at: 3585 3585
Size: 8809
PmeI GTTT'AAAC
Cuts at: 6113 6113
Size: 8809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6878 6910 8391 8423 6878
Size: 32 1481 32 7264
Fragments arranged by size:
7264 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8809
PsiI TTA'TAA
Cuts at: 3962 6379 3962
Size: 2417 6392
PstI C_TGCA'G
Cuts at: 830 2022 3381 3901 6031 6161 830
Size: 1192 1359 520 2130 130 3478
Fragments arranged by size:
3478 2130 1359 1192 520 130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8809
PvuII CAG'CTG
Cuts at: 5306 6026 5306
Size: 720 8089
SacI G_AGCT'C
Cuts at: 2465 3487 5340 2465
Size: 1022 1853 5934
Fragments arranged by size:
5934 1853 1022
SalI G'TCGA_C
Cuts at: 1936 6151 1936
Size: 4215 4594
SapI GCTCTTCn'nnn_
Cuts at: 3203 3203
Size: 8809
SbfI CC_TGCA'GG
Cuts at: 6161 6161
Size: 8809
ScaI AGT'ACT
Cuts at: 5706 7057 8769 5706
Size: 1351 1712 5746
Fragments arranged by size:
5746 1712 1351
SfcI C'TryA_G
Cuts at: 826 1045 2018 2677 3332 3377 3897 4220 4426
Size: 219 973 659 655 45 520 323 206
Cuts at: 4426 5715 6027 6157 7292 7973 8164 826
Size: 1289 312 130 1135 681 191 1471
Fragments arranged by size:
1471 1289 1135 973 681 659 655 520
323 312 219 206 191 130 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8809
SmaI CCC'GGG
Cuts at: 1872 6136 1872
Size: 4264 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2324 3007 3818 6913 7814 8076
Size: 197 926 381 683 811 3095 901 262
Cuts at: 8076 820
Size: 1553
Fragments arranged by size:
3095 1553 926 901 811 683 381 262
197
SnaBI TAC'GTA
Cuts at: 493 6600 493
Size: 6107 2702
SpeI A'CTAG_T
Cuts at: 152 5227 152
Size: 5075 3734
SphI G_CATG'C
Cuts at: 1132 6167 1132
Size: 5035 3774
SspI AAT'ATT
Cuts at: 5 52 6733 5
Size: 47 6681 2081
Fragments arranged by size:
6681 2081 47
StuI AGG'CCT
Cuts at: 1517 2766 1517
Size: 1249 7560
StyI C'CwwG_G
Cuts at: 513 1065 2004 2937 3477 3678 4074 4458 5183
Size: 552 939 933 540 201 396 384 725
Cuts at: 5183 5346 6248 513
Size: 163 902 3074
Fragments arranged by size:
3074 939 933 902 725 552 540 396
384 201 163
SwaI ATTT'AAAT
Cuts at: 5868 5868
Size: 8809
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2299 2969 5741 5876 6836 6853 7006
Size: 1255 719 670 2772 135 960 17 153
Cuts at: 7006 7191 7636 325
Size: 185 445 1498
Fragments arranged by size:
2772 1498 1255 960 719 670 445 185
153 135 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2473 4837 5704
Size: 274 80 33 51 1443 496 2364 867
Cuts at: 5704 7055 8767 96
Size: 1351 1712 138
Fragments arranged by size:
2364 1712 1443 1351 867 496 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8769 8801 8769
Size: 32 8777
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8809
XbaI T'CTAG_A
Cuts at: 6145 6145
Size: 8809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3484 3676 5093 5274 5481 3484
Size: 192 1417 181 207 6812
Fragments arranged by size:
6812 1417 207 192 181
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8809
XmaI C'CCGG_G
Cuts at: 1870 6134 1870
Size: 4264 4545
XmnI GAAnn'nnTTC
Cuts at: 3197 5706 6938 3197
Size: 2509 1232 5068
Fragments arranged by size:
5068 2509 1232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5538 6617 276
Size: 53 83 186 936 4004 1079 2468
Fragments arranged by size:
4004 2468 1079 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI
DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI