Restriction map for
pFN21AA1198
(Circular) (Six-base) MAPSORT of: pFN21AA1198.seq Check: 3072 from: 1 to: 6292
pFN21AA1198 6292 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3517 3580 3635 1937
Size: 1580 63 55 4594
Fragments arranged by size:
4594 1580 63 55
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2932 3203 3361 3520 4355 5739 6007 1632
Size: 1300 271 158 159 835 1384 268 1917
Fragments arranged by size:
1917 1384 1300 835 271 268 159 158
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 3058 3092 5191 6168 1203
Size: 1855 34 2099 977 1327
Fragments arranged by size:
2099 1855 1327 977 34
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 3413 1166
Size: 2247 4045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6292
AlwNI CAG_nnn'CTG
Cuts at: 4035 5607 4035
Size: 1572 4720
ApaLI G'TGCA_C
Cuts at: 3140 3560 4349 5505 3140
Size: 420 789 1156 3927
Fragments arranged by size:
3927 1156 789 420
ApoI r'AATT_y
Cuts at: 1493 2014 2646 2730 3463 3486 3601 3827 3979
Size: 521 632 84 733 23 115 226 152
Cuts at: 3979 6088 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 733 632 521 226 152 115
84 23
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 3617 1870
Size: 73 105 1569 4545
Fragments arranged by size:
4545 1569 105 73
BamHI G'GATC_C
Cuts at: 2227 3622 3989 2227
Size: 1395 367 4530
Fragments arranged by size:
4530 1395 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2802 3613 5068 618
Size: 325 217 627 1015 811 1455 1842
Fragments arranged by size:
1842 1455 1015 811 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3611 1391
Size: 2220 4072
BbeI G_GCGC'C
Cuts at: 2806 2806
Size: 6292
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2488 3358 928
Size: 971 589 870 3862
Fragments arranged by size:
3862 971 870 589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2677 2711 4483 4517 1903
Size: 34 50 34 656 34 1772 34 3678
Fragments arranged by size:
3678 1772 656 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BfrBI ATG'CAT
Cuts at: 2503 2503
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4903 136
Size: 107 122 71 631 605 3231 1525
Fragments arranged by size:
3231 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2531 6287 2531
Size: 3756 2536
BlpI GC'TnA_GC
Cuts at: 3709 3709
Size: 6292
Bme1580I G_kGCm'C
Cuts at: 1790 3144 3564 4353 5509 1790
Size: 1354 420 789 1156 2573
Fragments arranged by size:
2573 1354 1156 789 420
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4023 4981 442
Size: 416 53 83 399 24 2606 958 1753
Fragments arranged by size:
2606 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2228 2608 2676 2760 2844 2928 3012
Size: 863 126 380 68 84 84 84 84
Cuts at: 3012 3432 3516 4952 1239
Size: 420 84 1436 2579
Fragments arranged by size:
2579 1436 863 420 380 126 84 84
84 84 84 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 3028 4417 5282 5580 2201
Size: 827 1389 865 298 2913
Fragments arranged by size:
2913 1389 865 827 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2515 4955 882
Size: 617 1016 2440 2219
Fragments arranged by size:
2440 2219 1016 617
BsaAI yAC'GTr
Cuts at: 493 1204 2961 3061 3559 4083 493
Size: 711 1757 100 498 524 2702
Fragments arranged by size:
2702 1757 711 524 498 100
BsaBI GATnn'nnATC
Cuts at: 6286 6286
Size: 6292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2803 4099 4481 5116
Size: 53 83 186 936 1270 1296 382 635
Cuts at: 5116 275
Size: 1451
Fragments arranged by size:
1451 1296 1270 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3074 4725 5123 5397 5544 5955 3074
Size: 1651 398 274 147 411 3411
Fragments arranged by size:
3411 1651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2159 2189 1178
Size: 30 165 30 756 30 5281
Fragments arranged by size:
5281 756 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2225 1186
Size: 880 159 5253
Fragments arranged by size:
5253 880 159
BseYI C'CCAG_C
Cuts at: 5495 5991 5495
Size: 496 5796
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5719
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3144 3564 4353 4438 5509 3144
Size: 420 789 85 1071 3927
Fragments arranged by size:
3927 1071 789 420 85
BsmI GAATG_Cn'
Cuts at: 2777 3350 3803 3896 2777
Size: 573 453 93 5173
Fragments arranged by size:
5173 573 453 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3144 3564 3611 4353 4438 5509 1391
Size: 399 1354 420 47 742 85 1071 2174
Fragments arranged by size:
2174 1354 1071 742 420 399 85 47
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 2974 4181 5911 2974
Size: 1207 1730 3355
Fragments arranged by size:
3355 1730 1207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3647 844
Size: 2803 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4179 1394
Size: 429 2356 3507
Fragments arranged by size:
3507 2356 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2939 4787 4961 65
Size: 1183 1691 1848 174 1396
Fragments arranged by size:
1848 1691 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4936 5123 1700
Size: 110 3126 187 2869
Fragments arranged by size:
3126 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6292
BstBI TT'CG_AA
Cuts at: 2632 2716 3052 3304 3388 5096 2632
Size: 84 336 252 84 1708 3828
Fragments arranged by size:
3828 1708 336 252 84 84
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 2227 2531 3622 3989 4374 4391 5843 5929
Size: 255 304 1091 367 385 17 1452 86
Cuts at: 5929 5941 6287 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1091 385 367 346 304 255
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2140 513
Size: 552 1075 4665
Fragments arranged by size:
4665 1075 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5553
Fragments arranged by size:
5553 683 56
BtsI GCAGTG_nn'
Cuts at: 2485 2790 3443 3808 4602 4622 2485
Size: 305 653 365 794 20 4155
Fragments arranged by size:
4155 794 653 365 305 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2581 2616 3337 3372 529
Size: 35 2017 35 721 35 3449
Fragments arranged by size:
3449 2017 721 35 35 35
DraI TTT'AAA
Cuts at: 3462 3596 3462
Size: 134 6158
DraIII CAC_nnn'GTG
Cuts at: 2378 2378
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 3739 4628
Size: 54 1425 50 275 18 403 1506 889
Cuts at: 4628 6257 8
Size: 1629 43
Fragments arranged by size:
1629 1506 1425 889 403 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2802 4222 1353
Size: 1449 1420 3423
Fragments arranged by size:
3423 1449 1420
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2228 2608 2676 2760 2844 2928
Size: 393 470 126 380 68 84 84 84
Cuts at: 2928 2932 3012 3203 3361 3432 3516 3520 4355
Size: 4 80 191 158 71 84 4 835
Cuts at: 4355 4952 5739 6007 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 835 787 597 470 393 380 268
191 158 126 84 84 84 84 80
71 68 4 4
EcoRI G'AATT_C
Cuts at: 2014 2646 2730 3486 3601 3979 2014
Size: 632 84 756 115 378 4327
Fragments arranged by size:
4327 756 632 378 115 84
EcoRV GAT'ATC
Cuts at: 2786 2786
Size: 6292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3302 3302
Size: 6292
FspI TGC'GCA
Cuts at: 2009 4798 2009
Size: 2789 3503
HaeII r_GCGC'y
Cuts at: 1678 2806 5439 1678
Size: 1128 2633 2531
Fragments arranged by size:
2633 2531 1128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2136 2159 2168 2191 2413
Size: 32 135 32 564 23 9 23 222
Cuts at: 2413 2445 4933 4965 5007 5039 1373
Size: 32 2488 32 42 32 2626
Fragments arranged by size:
2626 2488 564 222 135 42 32 32
32 32 32 23 23 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 3882 669
Size: 988 281 1698 246 3079
Fragments arranged by size:
3079 1698 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3447 748
Size: 2699 3593
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KasI G'GCGC_C
Cuts at: 2802 2802
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5406 5590 1305
Size: 4101 184 2007
Fragments arranged by size:
4101 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2235 10
Size: 54 1425 343 403 4067
Fragments arranged by size:
4067 1425 403 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2568 2587 3413 3427 4250 4609 4768
Size: 648 1402 19 826 14 823 359 159
Cuts at: 4768 518
Size: 2042
Fragments arranged by size:
2042 1402 826 823 648 359 159 19
14
MspA1I CmG'CkG
Cuts at: 1606 3708 4385 5533 5778 1606
Size: 2102 677 1148 245 2120
Fragments arranged by size:
2120 2102 1148 677 245
NarI GG'CG_CC
Cuts at: 2803 2803
Size: 6292
NcoI C'CATG_G
Cuts at: 513 1065 2140 513
Size: 552 1075 4665
Fragments arranged by size:
4665 1075 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NsiI A_TGCA'T
Cuts at: 2505 2505
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3096 3650 5195 1132
Size: 97 1867 554 1545 2229
Fragments arranged by size:
2229 1867 1545 554 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6292
PciI A'CATG_T
Cuts at: 3092 5191 3092
Size: 2099 4193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6292
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PmlI CAC'GTG
Cuts at: 3061 3559 3061
Size: 498 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PspXI vC'TCGA_Gb
Cuts at: 2048 2048
Size: 6292
PstI C_TGCA'G
Cuts at: 830 2480 3644 830
Size: 1650 1164 3478
Fragments arranged by size:
3478 1650 1164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SapI GCTCTTCn'nnn_
Cuts at: 2802 2802
Size: 6292
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SfcI C'TryA_G
Cuts at: 826 1045 2248 2476 3640 4775 5456 5647 826
Size: 219 1203 228 1164 1135 681 191 1471
Fragments arranged by size:
1471 1203 1164 1135 681 228 219 191
SfoI GGC'GCC
Cuts at: 2804 2804
Size: 6292
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3619 1872
Size: 1747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 2180 3043 4396 5297 5559
Size: 197 926 105 132 863 1353 901 262
Cuts at: 5559 820
Size: 1553
Fragments arranged by size:
1553 1353 926 901 863 262 197 132
105
SnaBI TAC'GTA
Cuts at: 493 4083 493
Size: 3590 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6292
StyI C'CwwG_G
Cuts at: 513 1065 2140 3731 513
Size: 552 1075 1591 3074
Fragments arranged by size:
3074 1591 1075 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4319 4336 4489 4674 5119 325
Size: 1255 2739 17 153 185 445 1498
Fragments arranged by size:
2739 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3475 4538 6250
Size: 274 80 33 51 1443 1498 1063 1712
Cuts at: 6250 96
Size: 138
Fragments arranged by size:
1712 1498 1443 1063 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6252 6284 6252
Size: 32 6260
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6292
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XhoI C'TCGA_G
Cuts at: 1943 2048 1943
Size: 105 6187
XmaI C'CCGG_G
Cuts at: 1870 3617 1870
Size: 1747 4545
XmnI GAAnn'nnTTC
Cuts at: 2609 2646 2777 2945 3234 4421 2609
Size: 37 131 168 289 1187 4480
Fragments arranged by size:
4480 1187 289 168 131 37
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI PacI
PfoI PpuMI PspOMI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI XcmI