Restriction map for
pFN21AA1193
(Circular) (Six-base) MAPSORT of: pFN21AA1193.seq Check: 5109 from: 1 to: 6340
pFN21AA1193 6340 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3683 1937
Size: 1746 4594
Acc65I G'GTAC_C
Cuts at: 3661 3661
Size: 6340
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4403 5787 6055 1632
Size: 2771 1384 268 1917
Fragments arranged by size:
2771 1917 1384 268
AfeI AGC'GCT
Cuts at: 3050 3050
Size: 6340
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 5239 6216 1203
Size: 4036 977 1327
Fragments arranged by size:
4036 1327 977
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 5069 5069
Size: 6340
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2273 2445 1184
Size: 1089 172 5079
Fragments arranged by size:
5079 1089 172
AlwNI CAG_nnn'CTG
Cuts at: 2815 3130 3304 4083 5655 2815
Size: 315 174 779 1572 3500
Fragments arranged by size:
3500 1572 779 315 174
ApaLI G'TGCA_C
Cuts at: 4397 5553 4397
Size: 1156 5184
ApoI r'AATT_y
Cuts at: 1493 3649 3875 4027 6136 1493
Size: 2156 226 152 2109 1697
Fragments arranged by size:
2156 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2194 3593 3665 1870
Size: 73 114 137 1399 72 4545
Fragments arranged by size:
4545 1399 137 114 73 72
BamHI G'GATC_C
Cuts at: 3478 3670 4037 3478
Size: 192 367 5781
Fragments arranged by size:
5781 367 192
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2199 3661 5116 618
Size: 325 217 627 412 1462 1455 1842
Fragments arranged by size:
1842 1462 1455 627 412 325 217
BanII G_rGCy'C
Cuts at: 1391 2120 2901 3246 3612 3659 1391
Size: 729 781 345 366 47 4072
Fragments arranged by size:
4072 781 729 366 345 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2523 2679 928
Size: 971 624 156 4589
Fragments arranged by size:
4589 971 624 156
BbvCI CC'TCA_GC
Cuts at: 2624 2772 3470 2624
Size: 148 698 5494
Fragments arranged by size:
5494 698 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2021 2055 2374 2408 4531 4565 1903
Size: 34 84 34 319 34 2123 34 3678
Fragments arranged by size:
3678 2123 319 84 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4232 5442 4232
Size: 1210 5130
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4951 136
Size: 107 122 71 631 605 3279 1525
Fragments arranged by size:
3279 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2658 6335 2658
Size: 3677 2663
BlpI GC'TnA_GC
Cuts at: 3757 3757
Size: 6340
Bme1580I G_kGCm'C
Cuts at: 1790 2075 2204 3587 4401 5557 1790
Size: 285 129 1383 814 1156 2573
Fragments arranged by size:
2573 1383 1156 814 285 129
BmgBI CAC'GTC
Cuts at: 1318 2338 3553 1318
Size: 1020 1215 4105
Fragments arranged by size:
4105 1215 1020
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3198 4071 5029
Size: 416 53 83 399 24 1781 873 958
Cuts at: 5029 442
Size: 1753
Fragments arranged by size:
1781 1753 958 873 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2209 2514 2548 2578 2915 2035
Size: 174 305 34 30 337 5460
Fragments arranged by size:
5460 337 305 174 34 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2246 3439 5000 1239
Size: 903 104 1193 1561 2579
Fragments arranged by size:
2579 1561 1193 903 104
Bpu10I CC'TnA_GC
Cuts at: 2624 2772 3470 2624
Size: 148 698 5494
Fragments arranged by size:
5494 698 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4465 5330 5628 4465
Size: 865 298 5177
Fragments arranged by size:
5177 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2053 2118 3239 5003 882
Size: 617 554 65 1121 1764 2219
Fragments arranged by size:
2219 1764 1121 617 554 65
BsaAI yAC'GTr
Cuts at: 493 1204 3582 4131 493
Size: 711 2378 549 2702
Fragments arranged by size:
2702 2378 711 549
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2297 3399 4147 4529
Size: 53 83 186 936 764 1102 748 382
Cuts at: 4529 5164 275
Size: 635 1451
Fragments arranged by size:
1451 1102 936 764 748 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2487 3104 3388 3481 4773 5171 5445 5592 6003
Size: 617 284 93 1292 398 274 147 411
Cuts at: 6003 2487
Size: 2824
Fragments arranged by size:
2824 1292 617 411 398 284 274 147
93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2020 2050 2225 2255 2453
Size: 30 165 30 617 30 175 30 198
Cuts at: 2453 2483 2714 2744 2778 2808 3599 3629 1178
Size: 30 231 30 34 30 791 30 3889
Fragments arranged by size:
3889 791 617 231 198 175 165 34
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2008 2201 2234 2533 2570 2735 2864 2933
Size: 822 193 33 299 37 165 129 69
Cuts at: 2933 3500 1186
Size: 567 4026
Fragments arranged by size:
4026 822 567 299 193 165 129 69
37 33
BseYI C'CCAG_C
Cuts at: 2023 2693 3066 3079 3343 3358 3499 5543 6039
Size: 670 373 13 264 15 141 2044 496
Cuts at: 6039 2023
Size: 2324
Fragments arranged by size:
2324 2044 670 496 373 264 141 15
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2618 2944 1163
Size: 573 882 326 4559
Fragments arranged by size:
4559 882 573 326
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3228 3790 4551 5579 1540
Size: 275 186 1159 68 562 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 562 275 186 68
BsiHKAI G_wGCw'C
Cuts at: 2064 2940 4401 4486 5557 2064
Size: 876 1461 85 1071 2847
Fragments arranged by size:
2847 1461 1071 876 85
BsmI GAATG_Cn'
Cuts at: 3851 3944 3851
Size: 93 6247
BsmBI CGTCTCn'nnnn_
Cuts at: 2411 2411
Size: 6340
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2075 2120 2204 2901 2940 3246
Size: 399 274 11 45 84 697 39 306
Cuts at: 3246 3587 3612 3659 4401 4486 5557 1391
Size: 341 25 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 697 399 341 306 274
85 84 47 45 39 25 11
BspEI T'CCGG_A
Cuts at: 3104 3388 3481 6003 3104
Size: 284 93 2522 3441
Fragments arranged by size:
3441 2522 284 93
BspHI T'CATG_A
Cuts at: 4229 5959 4229
Size: 1730 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2640 3644 3695 844
Size: 1796 1004 51 3489
Fragments arranged by size:
3489 1796 1004 51
BsrBI CCG'CTC
Cuts at: 1394 1823 4227 1394
Size: 429 2404 3507
Fragments arranged by size:
3507 2404 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4835 5009 65
Size: 1183 3587 174 1396
Fragments arranged by size:
3587 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2643 3227 4984 5171 1700
Size: 110 833 584 1757 187 2869
Fragments arranged by size:
2869 1757 833 584 187 110
BsrGI T'GTAC_A
Cuts at: 96 2730 96
Size: 2634 3706
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 2462 2756 4400 5412 2462
Size: 294 1644 1012 3390
Fragments arranged by size:
3390 1644 1012 294
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6340
BstBI TT'CG_AA
Cuts at: 5144 5144
Size: 6340
BstEII G'GTnAC_C
Cuts at: 1184 3520 1184
Size: 2336 4004
BstXI CCAn_nnnn'nTGG
Cuts at: 3463 3463
Size: 6340
BstYI r'GATC_y
Cuts at: 1972 2148 2495 2658 3202 3478 3670 4037 4422
Size: 176 347 163 544 276 192 367 385
Cuts at: 4422 4439 5891 5977 5989 6335 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 544 385 367 347 346 276
192 176 163 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2112 3111 3417 513
Size: 552 939 108 999 306 3436
Fragments arranged by size:
3436 999 939 552 306 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5601
Fragments arranged by size:
5601 683 56
BtsI GCAGTG_nn'
Cuts at: 2109 3381 3856 4650 4670 2109
Size: 1272 475 794 20 3779
Fragments arranged by size:
3779 1272 794 475 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2085 2120 529
Size: 35 1521 35 4749
Fragments arranged by size:
4749 1521 35 35
DraI TTT'AAA
Cuts at: 3644 3644
Size: 6340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2696 5347 809
Size: 507 1380 2651 1802
Fragments arranged by size:
2651 1802 1380 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2763 3157 3225
Size: 54 1425 50 275 18 933 394 68
Cuts at: 3225 3351 3414 3567 3787 4676 6305 8
Size: 126 63 153 220 889 1629 43
Fragments arranged by size:
1629 1425 933 889 394 275 220 153
126 68 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 3225 3787 1537
Size: 275 1345 68 562 4090
Fragments arranged by size:
4090 1345 562 275 68
EarI CTCTTCn'nnn_
Cuts at: 1353 2403 2409 2483 2539 2571 2910 4270 1353
Size: 1050 6 74 56 32 339 1360 3423
Fragments arranged by size:
3423 1360 1050 339 74 56 32 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4927 5301 5447 387
Size: 1064 138 435 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2246 3439 4403 5000 5787 6055
Size: 393 510 104 1193 964 597 787 268
Cuts at: 6055 1239
Size: 1524
Fragments arranged by size:
1524 1193 964 787 597 510 393 268
104
EcoNI CCTnn'n_nnAGG
Cuts at: 2975 3434 2975
Size: 459 5881
EcoO109I rG'GnC_Cy
Cuts at: 2603 2689 2854 3127 2603
Size: 86 165 273 5816
Fragments arranged by size:
5816 273 165 86
EcoRI G'AATT_C
Cuts at: 3649 4027 3649
Size: 378 5962
FspI TGC'GCA
Cuts at: 2993 3197 4846 2993
Size: 204 1649 4487
Fragments arranged by size:
4487 1649 204
FspAI rTGC'GCAy
Cuts at: 2993 3197 2993
Size: 204 6136
HaeII r_GCGC'y
Cuts at: 1678 2816 3052 5487 1678
Size: 1138 236 2435 2531
Fragments arranged by size:
2531 2435 1138 236
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 2138 2170 2225
Size: 32 135 32 448 32 86 32 55
Cuts at: 2225 2257 2408 2440 3347 3379 3599 3631 4981
Size: 32 151 32 907 32 220 32 1350
Cuts at: 4981 5013 5055 5087 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 907 448 220 151 135 86
55 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3684 3930 669
Size: 988 281 1746 246 3079
Fragments arranged by size:
3079 1746 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6340
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KpnI G_GTAC'C
Cuts at: 3665 3665
Size: 6340
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2496 2571 3024 5454 5638 1305
Size: 1191 75 453 2430 184 2007
Fragments arranged by size:
2430 2007 1191 453 184 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4518
Fragments arranged by size:
4518 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4298 4657 4816 518
Size: 648 3132 359 159 2042
Fragments arranged by size:
3132 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2680 3304 3320 3377 3474 3756 4433 5581
Size: 1074 624 16 57 97 282 677 1148
Cuts at: 5581 5826 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1074 677 624 282 245 97
57 16
NcoI C'CATG_G
Cuts at: 513 1065 2004 2112 513
Size: 552 939 108 4741
Fragments arranged by size:
4741 939 552 108
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6340
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6340
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 2260 3698 5243 1132
Size: 97 1031 1438 1545 2229
Fragments arranged by size:
2229 1545 1438 1031 97
PasI CC'CwG_GG
Cuts at: 1348 2075 1348
Size: 727 5613
PciI A'CATG_T
Cuts at: 5239 5239
Size: 6340
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3130 3304 1108
Size: 2022 174 4144
Fragments arranged by size:
4144 2022 174
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PmlI CAC'GTG
Cuts at: 3582 3582
Size: 6340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 5922 5954 4409
Size: 32 1481 32 4795
Fragments arranged by size:
4795 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2603 2689 3127 2603
Size: 86 438 5816
Fragments arranged by size:
5816 438 86
PshAI GACnn'nnGTC
Cuts at: 1613 3268 1613
Size: 1655 4685
PsiI TTA'TAA
Cuts at: 3910 3910
Size: 6340
PspXI vC'TCGA_Gb
Cuts at: 2057 2194 2057
Size: 137 6203
PstI C_TGCA'G
Cuts at: 830 3692 830
Size: 2862 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
PvuII CAG'CTG
Cuts at: 2680 3304 3377 2680
Size: 624 73 5643
Fragments arranged by size:
5643 624 73
SalI G'TCGA_C
Cuts at: 1936 3682 1936
Size: 1746 4594
SanDI GG'GwC_CC
Cuts at: 2689 2689
Size: 6340
SapI GCTCTTCn'nnn_
Cuts at: 2539 2539
Size: 6340
SbfI CC_TGCA'GG
Cuts at: 3692 3692
Size: 6340
ScaI AGT'ACT
Cuts at: 3023 4588 6300 3023
Size: 1565 1712 3063
Fragments arranged by size:
3063 1712 1565
SfcI C'TryA_G
Cuts at: 826 1045 2164 3688 4823 5504 5695 826
Size: 219 1119 1524 1135 681 191 1471
Fragments arranged by size:
1524 1471 1135 1119 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 3595 3667 1872
Size: 1723 72 4545
Fragments arranged by size:
4545 1723 72
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 2194 4444 5345 5607 820
Size: 197 926 114 137 2250 901 262 1553
Fragments arranged by size:
2250 1553 926 901 262 197 137 114
SnaBI TAC'GTA
Cuts at: 493 4131 493
Size: 3638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 3698 1132
Size: 2566 3774
SspI AAT'ATT
Cuts at: 5 52 4264 5
Size: 47 4212 2081
Fragments arranged by size:
4212 2081 47
StuI AGG'CCT
Cuts at: 1517 2015 2192 2468 1517
Size: 498 177 276 5389
Fragments arranged by size:
5389 498 276 177
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 2214 3407 3435 3779 513
Size: 552 939 108 102 1193 28 344 3074
Fragments arranged by size:
3074 1193 939 552 344 108 102 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3508 4367 4384 4537 4722 5167 325
Size: 1255 1928 859 17 153 185 445 1498
Fragments arranged by size:
1928 1498 1255 859 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2730 3021 4586
Size: 274 80 33 51 1443 753 291 1565
Cuts at: 4586 6298 96
Size: 1712 138
Fragments arranged by size:
1712 1565 1443 753 291 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2453 2485 2778 2810 6300 6332 2453
Size: 32 293 32 3490 32 2461
Fragments arranged by size:
3490 2461 293 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6340
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 3131 3409 2221
Size: 910 278 5152
Fragments arranged by size:
5152 910 278
XhoI C'TCGA_G
Cuts at: 1943 2057 2194 1943
Size: 114 137 6089
Fragments arranged by size:
6089 137 114
XmaI C'CCGG_G
Cuts at: 1870 3593 3665 1870
Size: 1723 72 4545
Fragments arranged by size:
4545 1723 72
XmnI GAAnn'nnTTC
Cuts at: 2234 3560 4469 2234
Size: 1326 909 4105
Fragments arranged by size:
4105 1326 909
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspOMI PsrI RsrII SacI SacII SexAI SfiI SfoI
SrfI SwaI