Restriction map for
pFN21AA1180
(Circular) (Six-base) MAPSORT of: pFN21AA1180.seq Check: 8847 from: 1 to: 5761
pFN21AA1180 5761 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 2253 3082 2253
Size: 829 4932
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2497 2798 2853 3824 5208 5476 1632
Size: 865 301 55 971 1384 268 1917
Fragments arranged by size:
1917 1384 971 865 301 268 55
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 2613 4660 5637 1203
Size: 1410 2047 977 1327
Fragments arranged by size:
2047 1410 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2298 1184
Size: 1114 4647
AlwNI CAG_nnn'CTG
Cuts at: 2792 3504 5076 2792
Size: 712 1572 3477
Fragments arranged by size:
3477 1572 712
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 3070 3296 3448 5557 1493
Size: 1577 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 1870
Size: 73 1143 4545
Fragments arranged by size:
4545 1143 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2253 2345 2659 3082 4537
Size: 325 217 627 466 92 314 423 1455
Cuts at: 4537 618
Size: 1842
Fragments arranged by size:
1842 1455 627 466 423 325 314 217
92
BanII G_rGCy'C
Cuts at: 1391 2078 3080 1391
Size: 687 1002 4072
Fragments arranged by size:
4072 1002 687
BbeI G_GCGC'C
Cuts at: 2349 2349
Size: 5761
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BbvCI CC'TCA_GC
Cuts at: 2721 2914 2721
Size: 193 5568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3952 3986 1903
Size: 34 50 34 1931 34 3678
Fragments arranged by size:
3678 1931 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 3178 3178
Size: 5761
Bme1580I G_kGCm'C
Cuts at: 1790 2664 2913 3822 4978 1790
Size: 874 249 909 1156 2573
Fragments arranged by size:
2573 1156 909 874 249
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2458 3492 4450
Size: 416 53 83 399 24 1041 1034 958
Cuts at: 4450 442
Size: 1753
Fragments arranged by size:
1753 1041 1034 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2814 4421 1239
Size: 1575 1607 2579
Fragments arranged by size:
2579 1607 1575
Bpu10I CC'TnA_GC
Cuts at: 2721 2914 2721
Size: 193 5568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3886 4751 5049 3886
Size: 865 298 4598
Fragments arranged by size:
4598 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2625 4424 882
Size: 617 1126 1799 2219
Fragments arranged by size:
2219 1799 1126 617
BsaAI yAC'GTr
Cuts at: 493 1204 2206 3552 493
Size: 711 1002 1346 2702
Fragments arranged by size:
2702 1346 1002 711
BsaBI GATnn'nnATC
Cuts at: 2333 5755 2333
Size: 3422 2339
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2234 2346 3568 3950
Size: 53 83 186 936 701 112 1222 382
Cuts at: 3950 4585 275
Size: 635 1451
Fragments arranged by size:
1451 1222 936 701 635 382 186 112
83 53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2343 2373 2892 2922 1178
Size: 30 165 30 940 30 519 30 4017
Fragments arranged by size:
4017 940 519 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2537 2916 1186
Size: 1351 379 4031
Fragments arranged by size:
4031 1351 379
BseYI C'CCAG_C
Cuts at: 2016 2095 2294 3025 4964 5460 2016
Size: 79 199 731 1939 496 2317
Fragments arranged by size:
2317 1939 731 496 199 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2324 2570 2983 1163
Size: 573 588 246 413 3941
Fragments arranged by size:
3941 588 573 413 246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2502 3822 3907 4978 2502
Size: 1320 85 1071 3285
Fragments arranged by size:
3285 1320 1071 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2502 2664 2913 3080 3822 3907
Size: 399 288 424 162 249 167 742 85
Cuts at: 3907 4978 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 424 399 288 249 167
162 85
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2604 3116 844
Size: 1760 512 3489
Fragments arranged by size:
3489 1760 512
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2868 4256 4430 65
Size: 1183 1620 1388 174 1396
Fragments arranged by size:
1620 1396 1388 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2559 2646 4405 4592 1700
Size: 110 749 87 1759 187 2869
Fragments arranged by size:
2869 1759 749 187 110 87
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 3241
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstXI CCAn_nnnn'nTGG
Cuts at: 2974 2974
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756
Size: 1119 367 385 17 1452 86 12 346
Cuts at: 5756 1972
Size: 1977
Fragments arranged by size:
1977 1452 1119 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2247 2247
Size: 5761
BtgI C'CryG_G
Cuts at: 513 1065 2268 3045 513
Size: 552 1203 777 3229
Fragments arranged by size:
3229 1203 777 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 2913 2946 3277 4071 4091 2913
Size: 33 331 794 20 4583
Fragments arranged by size:
4583 794 331 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DraIII CAC_nnn'GTG
Cuts at: 2194 2194
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 3208 4097
Size: 54 1425 50 275 18 534 844 889
Cuts at: 4097 5726 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 844 534 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2516 2609 3691 1353
Size: 1163 93 1082 3423
Fragments arranged by size:
3423 1163 1082 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2497 2798 2814 2853 3824 4421 5208
Size: 393 865 301 16 39 971 597 787
Cuts at: 5208 5476 1239
Size: 268 1524
Fragments arranged by size:
1524 971 865 787 597 393 301 268
39 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2396
Size: 5761
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2349 4908 1678
Size: 671 2559 2531
Fragments arranged by size:
2559 2531 671
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373
Size: 32 135 32 2830 32 42 32 2626
Fragments arranged by size:
2830 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3037 3105 3351 669
Size: 988 281 1099 68 246 3079
Fragments arranged by size:
3079 1099 988 281 246 68
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KasI G'GCGC_C
Cuts at: 2345 2345
Size: 5761
KpnI G_GTAC'C
Cuts at: 2257 3086 2257
Size: 829 4932
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4875 5059 1305
Size: 3570 184 2007
Fragments arranged by size:
3570 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2366 10
Size: 54 1425 343 534 3405
Fragments arranged by size:
3405 1425 534 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2208 2339 3050 3719 4078 4237 518
Size: 648 1042 131 711 669 359 159 2042
Fragments arranged by size:
2042 1042 711 669 648 359 159 131
MspA1I CmG'CkG
Cuts at: 1606 2278 2682 2720 3177 3854 5002 5247 1606
Size: 672 404 38 457 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 457 404 245 38
NarI GG'CG_CC
Cuts at: 2346 2346
Size: 5761
NcoI C'CATG_G
Cuts at: 513 1065 2268 3045 513
Size: 552 1203 777 3229
Fragments arranged by size:
3229 1203 777 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2063 2173 2617 3119 4664 1132
Size: 97 834 110 444 502 1545 2229
Fragments arranged by size:
2229 1545 834 502 444 110 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 2613 4660 2613
Size: 2047 3714
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2119 3046 1108
Size: 1011 927 3823
Fragments arranged by size:
3823 1011 927
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PmlI CAC'GTG
Cuts at: 2206 2206
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2601 3113 830
Size: 1771 512 3478
Fragments arranged by size:
3478 1771 512
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2278 2278
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SapI GCTCTTCn'nnn_
Cuts at: 2609 2609
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SexAI A'CCwGG_T
Cuts at: 2391 2391
Size: 5761
SfcI C'TryA_G
Cuts at: 826 1045 2597 3109 4244 4925 5116 826
Size: 219 1552 512 1135 681 191 1471
Fragments arranged by size:
1552 1471 1135 681 512 219 191
SfoI GGC'GCC
Cuts at: 2347 2347
Size: 5761
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3865 4766 5028 820
Size: 197 926 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 2996 1517
Size: 1479 4282
StyI C'CwwG_G
Cuts at: 513 1065 2082 2268 3045 3200 513
Size: 552 1017 186 777 155 3074
Fragments arranged by size:
3074 1017 777 552 186 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2901 3017 3788 3805 3958 4143 4588
Size: 1255 1321 116 771 17 153 185 445
Cuts at: 4588 325
Size: 1498
Fragments arranged by size:
1498 1321 1255 771 445 185 153 116
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 4007 5719
Size: 274 80 33 51 1443 639 1391 1712
Cuts at: 5719 96
Size: 138
Fragments arranged by size:
1712 1443 1391 639 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2211 2243 2343 2367 2375 2399 2892 2924 5721
Size: 32 100 24 8 24 493 32 2797
Cuts at: 5721 5753 2211
Size: 32 2219
Fragments arranged by size:
2797 2219 493 100 32 32 32 24
24 8
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2191 2275 2191
Size: 84 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SrfI SwaI