Restriction map for
pFN21AA1179
(Circular) (Six-base) MAPSORT of: pFN21AA1179.seq Check: 1096 from: 1 to: 9952
pFN21AA1179 9952 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4262 4439 4262
Size: 177 9775
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7762 278
Size: 53 83 186 936 6226 2468
Fragments arranged by size:
6226 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2280 6723 7295 1937
Size: 343 4443 572 4594
Fragments arranged by size:
4594 4443 572 343
Acc65I G'GTAC_C
Cuts at: 2525 7273 2525
Size: 4748 5204
AclI AA'CG_TT
Cuts at: 1563 3103 8079 8452 1563
Size: 1540 4976 373 3063
Fragments arranged by size:
4976 3063 1540 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2418 3493 4209 5148 5508 8015 9399 9667
Size: 786 1075 716 939 360 2507 1384 268
Cuts at: 9667 1632
Size: 1917
Fragments arranged by size:
2507 1917 1384 1075 939 786 716 360
268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9755
AflIII A'CryG_T
Cuts at: 1203 4842 5193 5269 8851 9828 1203
Size: 3639 351 76 3582 977 1327
Fragments arranged by size:
3639 3582 1327 977 351 76
AgeI A'CCGG_T
Cuts at: 8783 8783
Size: 9952
AhdI GACnn_n'nnGTC
Cuts at: 8681 8681
Size: 9952
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4632 5389 1184
Size: 3448 757 5747
Fragments arranged by size:
5747 3448 757
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2714 2746 4721 4753 2714
Size: 32 1975 32 7913
Fragments arranged by size:
7913 1975 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4243 6976 6988 7695 9267 4243
Size: 2733 12 707 1572 4928
Fragments arranged by size:
4928 2733 1572 707 12
ApaI G_GGCC'C
Cuts at: 5683 5683
Size: 9952
ApaLI G'TGCA_C
Cuts at: 8009 9165 8009
Size: 1156 8796
ApoI r'AATT_y
Cuts at: 1493 2093 2159 2502 3305 5384 5474 5495 7100
Size: 600 66 343 803 2079 90 21 1605
Cuts at: 7100 7145 7199 7261 7487 7639 9748 1493
Size: 45 54 62 226 152 2109 1697
Fragments arranged by size:
2109 2079 1697 1605 803 600 343 226
152 90 66 62 54 45 21
AseI AT'TA_AT
Cuts at: 160 1032 3339 4626 8506 160
Size: 872 2307 1287 3880 1606
Fragments arranged by size:
3880 2307 1606 1287 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9952
AvaI C'yCGr_G
Cuts at: 1870 1943 3039 4017 4368 5162 5429 5625 6993
Size: 73 1096 978 351 794 267 196 1368
Cuts at: 6993 7277 1870
Size: 284 4545
Fragments arranged by size:
4545 1368 1096 978 794 351 284 267
196 73
AvrII C'CTAG_G
Cuts at: 3091 3091
Size: 9952
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2517 2550 2727 2760 2517
Size: 33 177 33 9709
Fragments arranged by size:
9709 177 33 33
BamHI G'GATC_C
Cuts at: 2808 7282 7649 2808
Size: 4474 367 5111
Fragments arranged by size:
5111 4474 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2525 3443 3664 4200 4679
Size: 325 217 627 738 918 221 536 479
Cuts at: 4679 6889 7007 7273 8728 618
Size: 2210 118 266 1455 1842
Fragments arranged by size:
2210 1842 1455 918 738 627 536 479
325 266 221 217 118
BanII G_rGCy'C
Cuts at: 1391 2039 4338 4806 5683 6066 6418 6476 7054
Size: 648 2299 468 877 383 352 58 578
Cuts at: 7054 7227 7271 1391
Size: 173 44 4072
Fragments arranged by size:
4072 2299 877 648 578 468 383 352
173 58 44
BbeI G_GCGC'C
Cuts at: 7011 7011
Size: 9952
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 6177 928
Size: 971 4278 4703
Fragments arranged by size:
4703 4278 971
BbvCI CC'TCA_GC
Cuts at: 4797 5577 4797
Size: 780 9172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 8143 8177 1903
Size: 34 50 34 6122 34 3678
Fragments arranged by size:
6122 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4024 6862 7067 7844 9054 4024
Size: 2838 205 777 1210 4922
Fragments arranged by size:
4922 2838 1210 777 205
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9952
BfrBI ATG'CAT
Cuts at: 3231 4772 4896 3231
Size: 1541 124 8287
Fragments arranged by size:
8287 1541 124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5630 7016 8563
Size: 107 122 71 631 605 3958 1386 1547
Cuts at: 8563 136
Size: 1525
Fragments arranged by size:
3958 1547 1525 1386 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4714 5080 9947 4714
Size: 366 4867 4719
Fragments arranged by size:
4867 4719 366
BlpI GC'TnA_GC
Cuts at: 2033 5565 6903 7369 2033
Size: 3532 1338 466 4616
Fragments arranged by size:
4616 3532 1338 466
Bme1580I G_kGCm'C
Cuts at: 1790 2918 4203 4684 5683 5766 8013 9169 1790
Size: 1128 1285 481 999 83 2247 1156 2573
Fragments arranged by size:
2573 2247 1285 1156 1128 999 481 83
BmgBI CAC'GTC
Cuts at: 1318 3613 4064 7192 1318
Size: 2295 451 3128 4078
Fragments arranged by size:
4078 3128 2295 451
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4547 5869 6955
Size: 416 53 83 399 24 3130 1322 1086
Cuts at: 6955 7172 7683 8641 442
Size: 217 511 958 1753
Fragments arranged by size:
3130 1753 1322 1086 958 511 416 399
217 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4307 4385 1056
Size: 3251 78 6623
Fragments arranged by size:
6623 3251 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 5754 6394 6548 6592 3065
Size: 2689 640 154 44 6425
Fragments arranged by size:
6425 2689 640 154 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2583 3734 4232 4511 5141 5480 5795 5892
Size: 1344 1151 498 279 630 339 315 97
Cuts at: 5892 6564 6998 8612 1239
Size: 672 434 1614 2579
Fragments arranged by size:
2579 1614 1344 1151 672 630 498 434
339 315 279 97
Bpu10I CC'TnA_GC
Cuts at: 4797 5577 6147 4797
Size: 780 570 8602
Fragments arranged by size:
8602 780 570
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3035 3916 5229 5399 6188 8077 8942 9240 3035
Size: 881 1313 170 789 1889 865 298 3747
Fragments arranged by size:
3747 1889 1313 881 865 789 298 170
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3983 4894 5680 8615 882
Size: 617 2484 911 786 2935 2219
Fragments arranged by size:
2935 2484 2219 911 786 617
BsaAI yAC'GTr
Cuts at: 493 1204 2467 5272 7743 493
Size: 711 1263 2805 2471 2702
Fragments arranged by size:
2805 2702 2471 1263 711
BsaBI GATnn'nnATC
Cuts at: 9946 9946
Size: 9952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7008 7759 8141 8776
Size: 53 83 186 936 5475 751 382 635
Cuts at: 8776 275
Size: 1451
Fragments arranged by size:
5475 1451 936 751 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3516 4725 6916 8385 8783 9057 9204 9615 3516
Size: 1209 2191 1469 398 274 147 411 3853
Fragments arranged by size:
3853 2191 1469 1209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2714 2744 3486 3516 3566
Size: 30 165 30 1311 30 742 30 50
Cuts at: 3566 3596 5135 5165 5360 5390 7004 7034 1178
Size: 30 1539 30 195 30 1614 30 4096
Fragments arranged by size:
4096 1614 1539 1311 742 195 165 50
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3494 4145 4277 4589 4814 5075 5316 5654
Size: 2308 651 132 312 225 261 241 338
Cuts at: 5654 6134 6605 1186
Size: 480 471 4533
Fragments arranged by size:
4533 2308 651 480 471 338 312 261
241 225 132
BseYI C'CCAG_C
Cuts at: 3139 4204 4331 5332 5765 6024 6510 6708 6768
Size: 1065 127 1001 433 259 486 198 60
Cuts at: 6768 6898 7229 9155 9651 3139
Size: 130 331 1926 496 3440
Fragments arranged by size:
3440 1926 1065 1001 496 486 433 331
259 198 130 127 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2089 4619 6072 6314 1163
Size: 573 353 2530 1453 242 4801
Fragments arranged by size:
4801 2530 1453 573 353 242
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7402 8163 9191 1540
Size: 275 186 5401 761 1028 2301
Fragments arranged by size:
5401 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3051 4063 4652 4806 6066 6476 7040 8013 8098
Size: 1012 589 154 1260 410 564 973 85
Cuts at: 8098 9169 3051
Size: 1071 3834
Fragments arranged by size:
3834 1260 1071 1012 973 589 564 410
154 85
BsmI GAATG_Cn'
Cuts at: 4126 4514 6782 7463 7556 4126
Size: 388 2268 681 93 6522
Fragments arranged by size:
6522 2268 681 388 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2918 3051 4063 4203 4338 4652
Size: 399 249 879 133 1012 140 135 314
Cuts at: 4652 4684 4806 5683 5766 6066 6418 6476 7040
Size: 32 122 877 83 300 352 58 564
Cuts at: 7040 7054 7227 7271 8013 8098 9169 1391
Size: 14 173 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 1012 879 877 742 564 399
352 314 300 249 173 140 135 133
122 85 83 58 44 32 14
BspEI T'CCGG_A
Cuts at: 9615 9615
Size: 9952
BspHI T'CATG_A
Cuts at: 3309 4518 7841 9571 3309
Size: 1209 3323 1730 3690
Fragments arranged by size:
3690 3323 1730 1209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2072 4262 4439 6973 7307 844
Size: 1228 2190 177 2534 334 3489
Fragments arranged by size:
3489 2534 2190 1228 334 177
BsrBI CCG'CTC
Cuts at: 1394 1823 2725 3718 5800 7839 1394
Size: 429 902 993 2082 2039 3507
Fragments arranged by size:
3507 2082 2039 993 902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2366 3202 3888 3930 6747 8447 8621
Size: 1183 1118 836 686 42 2817 1700 174
Cuts at: 8621 65
Size: 1396
Fragments arranged by size:
2817 1700 1396 1183 1118 836 686 174
42
BsrFI r'CCGG_y
Cuts at: 1700 1810 2365 8596 8783 1700
Size: 110 555 6231 187 2869
Fragments arranged by size:
6231 2869 555 187 110
BsrGI T'GTAC_A
Cuts at: 96 4845 5022 6403 96
Size: 4749 177 1381 3645
Fragments arranged by size:
4749 3645 1381 177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9952
BssSI C'ACGA_G
Cuts at: 4754 8012 9024 4754
Size: 3258 1012 5682
Fragments arranged by size:
5682 3258 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6101 6749 6988 794
Size: 5307 648 239 3758
Fragments arranged by size:
5307 3758 648 239
BstBI TT'CG_AA
Cuts at: 5478 8756 5478
Size: 3278 6674
BstEII G'GTnAC_C
Cuts at: 1184 2059 1184
Size: 875 9077
BstXI CCAn_nnnn'nTGG
Cuts at: 4298 5773 5830 6032 4298
Size: 1475 57 202 8218
Fragments arranged by size:
8218 1475 202 57
BstYI r'GATC_y
Cuts at: 1972 2808 3185 3418 4714 5080 6157 6453 7282
Size: 836 377 233 1296 366 1077 296 829
Cuts at: 7282 7649 8034 8051 9503 9589 9601 9947 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1296 1077 836 829 385 377
367 366 346 296 233 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2021 2040 2718 2021
Size: 19 678 9255
Fragments arranged by size:
9255 678 19
BtgI C'CryG_G
Cuts at: 513 1065 4137 5889 6669 6963 513
Size: 552 3072 1752 780 294 3502
Fragments arranged by size:
3502 3072 1752 780 552 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4901 487
Size: 683 56 3675 5538
Fragments arranged by size:
5538 3675 683 56
BtsI GCAGTG_nn'
Cuts at: 2871 3131 3599 4410 4731 7468 8262 8282 2871
Size: 260 468 811 321 2737 794 20 4541
Fragments arranged by size:
4541 2737 811 794 468 321 260 20
ClaI AT'CG_AT
Cuts at: 1554 6099 1554
Size: 4545 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2227 2262 529
Size: 35 1663 35 8219
Fragments arranged by size:
8219 1663 35 35
DraI TTT'AAA
Cuts at: 7256 7256
Size: 9952
DraIII CAC_nnn'GTG
Cuts at: 2924 4597 2924
Size: 1673 8279
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2120 2312 8959 809
Size: 507 804 192 6647 1802
Fragments arranged by size:
6647 1802 804 507 192
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2363 5235 7109
Size: 54 1425 50 275 18 533 2872 1874
Cuts at: 7109 7155 7399 8288 9917 8
Size: 46 244 889 1629 43
Fragments arranged by size:
2872 1874 1629 1425 889 533 275 244
54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7399 1537
Size: 275 5587 4090
Fragments arranged by size:
5587 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2192 2826 3072 3512 5214 5475 6539 6927
Size: 839 634 246 440 1702 261 1064 388
Cuts at: 6927 7882 1353
Size: 955 3423
Fragments arranged by size:
3423 1702 1064 955 839 634 440 388
261 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4044 5045 5951 8539 8913 9059
Size: 1064 138 2455 1001 906 2588 374 146
Cuts at: 9059 387
Size: 1280
Fragments arranged by size:
2588 2455 1280 1064 1001 906 374 146
138
EcoICRI GAG'CTC
Cuts at: 4804 6064 6474 4804
Size: 1260 410 8282
Fragments arranged by size:
8282 1260 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2418 2583 3493 3734 4209 4232 4511
Size: 393 786 165 910 241 475 23 279
Cuts at: 4511 5141 5148 5480 5508 5795 5892 6564 6998
Size: 630 7 332 28 287 97 672 434
Cuts at: 6998 8015 8612 9399 9667 1239
Size: 1017 597 787 268 1524
Fragments arranged by size:
1524 1017 910 787 786 672 630 597
475 434 393 332 287 279 268 241
165 97 28 23 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3095 4424 6086 3095
Size: 1329 1662 6961
Fragments arranged by size:
6961 1662 1329
EcoO109I rG'GnC_Cy
Cuts at: 2025 2999 3145 3598 3787 4486 5631 6020 2025
Size: 974 146 453 189 699 1145 389 5957
Fragments arranged by size:
5957 1145 974 699 453 389 189 146
EcoRI G'AATT_C
Cuts at: 2502 3305 5495 7261 7639 2502
Size: 803 2190 1766 378 4815
Fragments arranged by size:
4815 2190 1766 803 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3226 3523 3226
Size: 297 9655
FspI TGC'GCA
Cuts at: 5744 8458 5744
Size: 2714 7238
HaeII r_GCGC'y
Cuts at: 1678 7011 9099 1678
Size: 5333 2088 2531
Fragments arranged by size:
5333 2531 2088
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2543 2575 3486
Size: 32 135 32 575 32 364 32 911
Cuts at: 3486 3518 3991 3998 4023 4030 4459 4491 5360
Size: 32 473 7 25 7 429 32 869
Cuts at: 5360 5392 8593 8625 8667 8699 1373
Size: 32 3201 32 42 32 2626
Fragments arranged by size:
3201 2626 911 869 575 473 429 364
135 42 32 32 32 32 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2587 2988 5356 7296 7542 669
Size: 988 281 649 401 2368 1940 246 3079
Fragments arranged by size:
3079 2368 1940 988 649 401 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9952
HpaI GTT'AAC
Cuts at: 2587 7542 2587
Size: 4955 4997
KasI G'GCGC_C
Cuts at: 7007 7007
Size: 9952
KpnI G_GTAC'C
Cuts at: 2529 7277 2529
Size: 4748 5204
MfeI C'AATT_G
Cuts at: 4662 5371 7551 4662
Size: 709 2180 7063
Fragments arranged by size:
7063 2180 709
MluI A'CGCG_T
Cuts at: 9828 9828
Size: 9952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 9066 9250 1305
Size: 7761 184 2007
Fragments arranged by size:
7761 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5237 7157 10
Size: 54 1425 343 3405 1920 2805
Fragments arranged by size:
3405 2805 1920 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2520 3537 4142 4841 5018 6336 6464
Size: 648 1354 1017 605 699 177 1318 128
Cuts at: 6464 6624 7910 8269 8428 518
Size: 160 1286 359 159 2042
Fragments arranged by size:
2042 1354 1318 1286 1017 699 648 605
359 177 160 159 128
MspA1I CmG'CkG
Cuts at: 1606 2177 2741 5773 5928 5960 6028 6056 6661
Size: 571 564 3032 155 32 68 28 605
Cuts at: 6661 6973 7368 8045 9193 9438 1606
Size: 312 395 677 1148 245 2120
Fragments arranged by size:
3032 2120 1148 677 605 571 564 395
312 245 155 68 32 28
NaeI GCC'GGC
Cuts at: 2367 2367
Size: 9952
NarI GG'CG_CC
Cuts at: 7008 7008
Size: 9952
NcoI C'CATG_G
Cuts at: 513 1065 4137 6963 513
Size: 552 3072 2826 3502
Fragments arranged by size:
3502 3072 2826 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9952
NgoMIV G'CCGG_C
Cuts at: 2365 2365
Size: 9952
NheI G'CTAG_C
Cuts at: 1052 4303 4381 1052
Size: 3251 78 6623
Fragments arranged by size:
6623 3251 78
NotI GC'GGCC_GC
Cuts at: 7399 7399
Size: 9952
NsiI A_TGCA'T
Cuts at: 3233 4774 4898 3233
Size: 1541 124 8287
Fragments arranged by size:
8287 1541 124
NspI r_CATG'y
Cuts at: 1132 1229 2689 4147 4846 5197 6914 7310 8855
Size: 97 1460 1458 699 351 1717 396 1545
Cuts at: 8855 1132
Size: 2229
Fragments arranged by size:
2229 1717 1545 1460 1458 699 396 351
97
PasI CC'CwG_GG
Cuts at: 1348 3286 4675 1348
Size: 1938 1389 6625
Fragments arranged by size:
6625 1938 1389
PciI A'CATG_T
Cuts at: 4842 5193 8851 4842
Size: 351 3658 5943
Fragments arranged by size:
5943 3658 351
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4882 4945 6391 6976 1108
Size: 3774 63 1446 585 4084
Fragments arranged by size:
4084 3774 1446 585 63
PfoI T'CCnGG_A
Cuts at: 5340 5457 6366 6478 5340
Size: 117 909 112 8814
Fragments arranged by size:
8814 909 117 112
PmeI GTTT'AAAC
Cuts at: 7256 7256
Size: 9952
PmlI CAC'GTG
Cuts at: 5272 5272
Size: 9952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 6921 6953 8021 8053 9534 9566 2714
Size: 32 4175 32 1068 32 1481 32 3100
Fragments arranged by size:
4175 3100 1481 1068 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2025 2999 3145 3598 6020 2025
Size: 974 146 453 2422 5957
Fragments arranged by size:
5957 2422 974 453 146
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9952
PsiI TTA'TAA
Cuts at: 5712 6192 7522 5712
Size: 480 1330 8142
Fragments arranged by size:
8142 1330 480
PspOMI G'GGCC_C
Cuts at: 5679 5679
Size: 9952
PspXI vC'TCGA_Gb
Cuts at: 5162 5162
Size: 9952
PstI C_TGCA'G
Cuts at: 830 2644 4417 4782 5314 5368 5787 6061 6643
Size: 1814 1773 365 532 54 419 274 582
Cuts at: 6643 7304 830
Size: 661 3478
Fragments arranged by size:
3478 1814 1773 661 582 532 419 365
274 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9952
PvuII CAG'CTG
Cuts at: 2177 2741 5773 5960 6028 6056 6661 2177
Size: 564 3032 187 68 28 605 5468
Fragments arranged by size:
5468 3032 605 564 187 68 28
SacI G_AGCT'C
Cuts at: 4806 6066 6476 4806
Size: 1260 410 8282
Fragments arranged by size:
8282 1260 410
SalI G'TCGA_C
Cuts at: 1936 7294 1936
Size: 5358 4594
SanDI GG'GwC_CC
Cuts at: 3598 3598
Size: 9952
SapI GCTCTTCn'nnn_
Cuts at: 2192 2192
Size: 9952
SbfI CC_TGCA'GG
Cuts at: 4782 7304 4782
Size: 2522 7430
ScaI AGT'ACT
Cuts at: 8200 9912 8200
Size: 1712 8240
SexAI A'CCwGG_T
Cuts at: 4593 6552 4593
Size: 1959 7993
SfcI C'TryA_G
Cuts at: 826 1045 2640 3411 4280 4413 4718 4778 5138
Size: 219 1595 771 869 133 305 60 360
Cuts at: 5138 5227 5310 5364 5783 6015 6057 6639 6720
Size: 89 83 54 419 232 42 582 81
Cuts at: 6720 7300 8435 9116 9307 826
Size: 580 1135 681 191 1471
Fragments arranged by size:
1595 1471 1135 869 771 681 582 580
419 360 305 232 219 191 133 89
83 81 60 54 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 5630 5630
Size: 9952
SfoI GGC'GCC
Cuts at: 7009 7009
Size: 9952
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9952
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9952
SmaI CCC'GGG
Cuts at: 1872 4019 5627 7279 1872
Size: 2147 1608 1652 4545
Fragments arranged by size:
4545 2147 1652 1608
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3050 3895 5162 5244 5414 6203
Size: 197 926 1107 845 1267 82 170 789
Cuts at: 6203 8056 8957 9219 820
Size: 1853 901 262 1553
Fragments arranged by size:
1853 1553 1267 1107 926 901 845 789
262 197 170 82
SnaBI TAC'GTA
Cuts at: 493 7743 493
Size: 7250 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9952
SphI G_CATG'C
Cuts at: 1132 7310 1132
Size: 6178 3774
SspI AAT'ATT
Cuts at: 5 52 7876 5
Size: 47 7824 2081
Fragments arranged by size:
7824 2081 47
StuI AGG'CCT
Cuts at: 1517 5225 5694 6500 7019 1517
Size: 3708 469 806 519 4450
Fragments arranged by size:
4450 3708 806 519 469
StyI C'CwwG_G
Cuts at: 513 1065 2610 2844 2877 3091 4137 4194 4291
Size: 552 1545 234 33 214 1046 57 97
Cuts at: 4291 5517 5634 6756 6963 7391 513
Size: 1226 117 1122 207 428 3074
Fragments arranged by size:
3074 1545 1226 1122 1046 552 428 234
214 207 117 97 57 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2130 3749 4218 4667 4865 6224 6722
Size: 1255 550 1619 469 449 198 1359 498
Cuts at: 6722 6830 6937 7979 7996 8149 8334 8779 325
Size: 108 107 1042 17 153 185 445 1498
Fragments arranged by size:
1619 1498 1359 1255 1042 550 498 469
449 445 198 185 153 108 107 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4845 4993 5022
Size: 274 80 33 51 1443 2868 148 29
Cuts at: 5022 5877 6403 8198 9910 96
Size: 855 526 1795 1712 138
Fragments arranged by size:
2868 1795 1712 1443 855 526 274 148
138 80 51 33 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3027 3059 3597 3629 4757 4789 9912 9944 3027
Size: 32 538 32 1128 32 5123 32 3035
Fragments arranged by size:
5123 3035 1128 538 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2458 5597 6083 1316
Size: 1142 3139 486 5185
Fragments arranged by size:
5185 3139 1142 486
XbaI T'CTAG_A
Cuts at: 6838 7288 6838
Size: 450 9502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2632 2875 4393 2632
Size: 243 1518 8191
Fragments arranged by size:
8191 1518 243
XhoI C'TCGA_G
Cuts at: 1943 5162 1943
Size: 3219 6733
XmaI C'CCGG_G
Cuts at: 1870 4017 5625 7277 1870
Size: 2147 1608 1652 4545
Fragments arranged by size:
4545 2147 1652 1608
XmnI GAAnn'nnTTC
Cuts at: 3206 6006 6936 7145 8081 3206
Size: 2800 930 209 936 5077
Fragments arranged by size:
5077 2800 936 930 209
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7760 276
Size: 53 83 186 936 6226 2468
Fragments arranged by size:
6226 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BsiWI BsmBI BstZ17I EcoRV FseI FspAI
NruI PacI PsrI RsrII SacII SrfI SwaI