Restriction map for
pFN21AA1168
(Circular) (Six-base) MAPSORT of: pFN21AA1168.seq Check: 291 from: 1 to: 8539
pFN21AA1168 8539 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2902 3376 5631 2902
Size: 474 2255 5810
Fragments arranged by size:
5810 2255 474
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6349 278
Size: 53 83 186 936 4813 2468
Fragments arranged by size:
4813 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4185 5181 5882 1937
Size: 2248 996 701 4594
Fragments arranged by size:
4594 2248 996 701
Acc65I G'GTAC_C
Cuts at: 5860 5860
Size: 8539
AclI AA'CG_TT
Cuts at: 1563 4252 6666 7039 1563
Size: 2689 2414 373 3063
Fragments arranged by size:
3063 2689 2414 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4346 5036 5699 6602 7986 8254 1632
Size: 2714 690 663 903 1384 268 1917
Fragments arranged by size:
2714 1917 1384 903 690 663 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8342
AflIII A'CryG_T
Cuts at: 1203 2502 4336 5052 7438 8415 1203
Size: 1299 1834 716 2386 977 1327
Fragments arranged by size:
2386 1834 1327 1299 977 716
AgeI A'CCGG_T
Cuts at: 7370 7370
Size: 8539
AhdI GACnn_n'nnGTC
Cuts at: 3839 7268 3839
Size: 3429 5110
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8539
AlwNI CAG_nnn'CTG
Cuts at: 2587 4135 5065 6282 7854 2587
Size: 1548 930 1217 1572 3272
Fragments arranged by size:
3272 1572 1548 1217 930
ApaI G_GGCC'C
Cuts at: 5411 5411
Size: 8539
ApaLI G'TGCA_C
Cuts at: 3004 6596 7752 3004
Size: 3592 1156 3791
Fragments arranged by size:
3791 3592 1156
ApoI r'AATT_y
Cuts at: 1493 2894 3344 3950 4795 5848 6074 6226 8335
Size: 1401 450 606 845 1053 226 152 2109
Cuts at: 8335 1493
Size: 1697
Fragments arranged by size:
2109 1697 1401 1053 845 606 450 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2874 7093 160
Size: 872 1842 4219 1606
Fragments arranged by size:
4219 1842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8539
AvaI C'yCGr_G
Cuts at: 1870 1943 2274 3691 5864 1870
Size: 73 331 1417 2173 4545
Fragments arranged by size:
4545 2173 1417 331 73
BamHI G'GATC_C
Cuts at: 4745 4989 5869 6236 4745
Size: 244 880 367 7048
Fragments arranged by size:
7048 880 367 244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2917 3290 3640 5860 7315
Size: 325 217 627 1130 373 350 2220 1455
Cuts at: 7315 618
Size: 1842
Fragments arranged by size:
2220 1842 1455 1130 627 373 350 325
217
BanII G_rGCy'C
Cuts at: 1391 3093 3128 3171 3486 3582 5043 5411 5858
Size: 1702 35 43 315 96 1461 368 447
Cuts at: 5858 1391
Size: 4072
Fragments arranged by size:
4072 1702 1461 447 368 315 96 43
35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2339 3406 3815 4597 928
Size: 971 440 1067 409 782 4870
Fragments arranged by size:
4870 1067 971 782 440 409
BbvCI CC'TCA_GC
Cuts at: 4155 5283 4155
Size: 1128 7411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2383 2417 3720 3754 6730 6764 1903
Size: 34 446 34 1303 34 2976 34 3678
Fragments arranged by size:
3678 2976 1303 446 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4305 6431 7641 4305
Size: 2126 1210 5203
Fragments arranged by size:
5203 2126 1210
BclI T'GATC_A
Cuts at: 1548 3372 4174 1548
Size: 1824 802 5913
Fragments arranged by size:
5913 1824 802
BfrBI ATG'CAT
Cuts at: 2088 2156 2088
Size: 68 8471
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3490 3735 7150
Size: 107 122 71 631 605 1818 245 3415
Cuts at: 7150 136
Size: 1525
Fragments arranged by size:
3415 1818 1525 631 605 245 122 107
71
BglII A'GATC_T
Cuts at: 2322 4203 5712 8534 2322
Size: 1881 1509 2822 2327
Fragments arranged by size:
2822 2327 1881 1509
BlpI GC'TnA_GC
Cuts at: 5523 5956 5523
Size: 433 8106
Bme1580I G_kGCm'C
Cuts at: 1790 2922 3008 3645 5411 5676 6600 7756 1790
Size: 1132 86 637 1766 265 924 1156 2573
Fragments arranged by size:
2573 1766 1156 1132 924 637 265 86
BmgBI CAC'GTC
Cuts at: 1318 2017 3003 3223 4339 5053 5437 1318
Size: 699 986 220 1116 714 384 4420
Fragments arranged by size:
4420 1116 986 714 699 384 220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2602 2662 2769
Size: 416 53 83 399 24 1185 60 107
Cuts at: 2769 3022 3571 5560 6270 7228 442
Size: 253 549 1989 710 958 1753
Fragments arranged by size:
1989 1753 1185 958 710 549 416 399
253 107 83 60 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8539
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3253 4996 3253
Size: 1743 6796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2188 2243 2369 3980 4490 4502 4898 5015
Size: 949 55 126 1611 510 12 396 117
Cuts at: 5015 5225 7199 1239
Size: 210 1974 2579
Fragments arranged by size:
2579 1974 1611 949 510 396 210 126
117 55 12
Bpu10I CC'TnA_GC
Cuts at: 3127 4155 5283 3127
Size: 1028 1128 6383
Fragments arranged by size:
6383 1128 1028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2780 3619 6664 7529 7827 2780
Size: 839 3045 865 298 3492
Fragments arranged by size:
3492 3045 865 839 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2957 7202 882
Size: 617 1458 4245 2219
Fragments arranged by size:
4245 2219 1458 617
BsaAI yAC'GTr
Cuts at: 493 1204 6330 493
Size: 711 5126 2702
Fragments arranged by size:
5126 2702 711
BsaBI GATnn'nnATC
Cuts at: 8533 8533
Size: 8539
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4281 5427 6346 6728
Size: 53 83 186 936 2748 1146 919 382
Cuts at: 6728 7363 275
Size: 635 1451
Fragments arranged by size:
2748 1451 1146 936 919 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5211 5694 6972 7370 7644 7791 8202 5211
Size: 483 1278 398 274 147 411 5548
Fragments arranged by size:
5548 1278 483 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2775 2805 4439 4457 4469
Size: 30 165 30 1372 30 1634 18 12
Cuts at: 4469 4487 5318 5348 1178
Size: 18 831 30 4369
Fragments arranged by size:
4369 1634 1372 831 165 30 30 30
30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2723 2783 3170 3832 5090 5141 1186
Size: 1537 60 387 662 1258 51 4584
Fragments arranged by size:
4584 1537 1258 662 387 60 51
BseYI C'CCAG_C
Cuts at: 3933 4383 5272 5578 7742 8238 3933
Size: 450 889 306 2164 496 4234
Fragments arranged by size:
4234 2164 889 496 450 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2560 5654 1163
Size: 573 824 3094 4048
Fragments arranged by size:
4048 3094 824 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5695 5989 6750 7778 1540
Size: 275 186 3694 294 761 1028 2301
Fragments arranged by size:
3694 2301 1028 761 294 275 186
BsiHKAI G_wGCw'C
Cuts at: 2974 3008 3093 3171 3393 5043 6600 6685 7756
Size: 34 85 78 222 1650 1557 85 1071
Cuts at: 7756 2974
Size: 3757
Fragments arranged by size:
3757 1650 1557 1071 222 85 85 78
34
BsmI GAATG_Cn'
Cuts at: 2158 5373 6050 6143 2158
Size: 3215 677 93 4554
Fragments arranged by size:
4554 3215 677 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2922 2974 3008 3093 3128 3171 3393
Size: 399 1132 52 34 85 35 43 222
Cuts at: 3393 3486 3582 3645 5043 5411 5676 5858 6600
Size: 93 96 63 1398 368 265 182 742
Cuts at: 6600 6685 7756 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1398 1132 1071 742 399 368 265
222 182 96 93 85 85 63 52
43 35 34
BspEI T'CCGG_A
Cuts at: 5211 8202 5211
Size: 2991 5548
BspHI T'CATG_A
Cuts at: 2370 6428 8158 2370
Size: 4058 1730 2751
Fragments arranged by size:
4058 2751 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 2902 3376 4723 5394 5631 5894 844
Size: 1214 844 474 1347 671 237 263 3489
Fragments arranged by size:
3489 1347 1214 844 671 474 263 237
BsrBI CCG'CTC
Cuts at: 1394 1823 2407 5245 5269 5329 6426 1394
Size: 429 584 2838 24 60 1097 3507
Fragments arranged by size:
3507 2838 1097 584 429 60 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 2169 3449 3783 4761 5086 5295 7034
Size: 1183 921 1280 334 978 325 209 1739
Cuts at: 7034 7208 65
Size: 174 1396
Fragments arranged by size:
1739 1396 1280 1183 978 921 334 325
209 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4491 5460 7183 7370 1700
Size: 110 2681 969 1723 187 2869
Fragments arranged by size:
2869 2681 1723 969 187 110
BsrGI T'GTAC_A
Cuts at: 96 2253 3753 4086 96
Size: 2157 1500 333 4549
Fragments arranged by size:
4549 2157 1500 333
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8539
BssSI C'ACGA_G
Cuts at: 6599 7611 6599
Size: 1012 7527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2917 4507 794
Size: 2123 1590 4826
Fragments arranged by size:
4826 2123 1590
BstBI TT'CG_AA
Cuts at: 3544 7343 3544
Size: 3799 4740
BstEII G'GTnAC_C
Cuts at: 1184 2359 5309 1184
Size: 1175 2950 4414
Fragments arranged by size:
4414 2950 1175
BstXI CCAn_nnnn'nTGG
Cuts at: 2644 2644
Size: 8539
BstYI r'GATC_y
Cuts at: 1972 2322 2602 3057 4081 4203 4745 4989 5712
Size: 350 280 455 1024 122 542 244 723
Cuts at: 5712 5869 6236 6621 6638 8090 8176 8188 8534
Size: 157 367 385 17 1452 86 12 346
Cuts at: 8534 1972
Size: 1977
Fragments arranged by size:
1977 1452 1024 723 542 455 385 367
350 346 280 244 157 122 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3815 5066 3815
Size: 1251 7288
BtgI C'CryG_G
Cuts at: 513 1065 3294 3969 4224 5760 513
Size: 552 2229 675 255 1536 3292
Fragments arranged by size:
3292 2229 1536 675 552 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4837 5553 487
Size: 683 56 3611 716 3473
Fragments arranged by size:
3611 3473 716 683 56
BtsI GCAGTG_nn'
Cuts at: 3923 4240 4319 5534 6055 6849 6869 3923
Size: 317 79 1215 521 794 20 5593
Fragments arranged by size:
5593 1215 794 521 317 79 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8504
DraI TTT'AAA
Cuts at: 4123 5843 4123
Size: 1720 6819
DraIII CAC_nnn'GTG
Cuts at: 2670 4570 2670
Size: 1900 6639
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7546 809
Size: 507 6230 1802
Fragments arranged by size:
6230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 2949 4585
Size: 54 1425 50 275 18 816 303 1636
Cuts at: 4585 5688 5826 5986 6875 8504 8
Size: 1103 138 160 889 1629 43
Fragments arranged by size:
1636 1629 1425 1103 889 816 303 275
160 138 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5986 1537
Size: 275 4174 4090
Fragments arranged by size:
4174 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2861 2976 3176 3727 4883 5096 6469
Size: 699 809 115 200 551 1156 213 1373
Cuts at: 6469 1353
Size: 3423
Fragments arranged by size:
3423 1373 1156 809 699 551 213 200
115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 7126 7500 7646 387
Size: 1064 138 2975 2562 374 146 1280
Fragments arranged by size:
2975 2562 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3091 3169 5041 3091
Size: 78 1872 6589
Fragments arranged by size:
6589 1872 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2243 2369 3980 4346 4490 4502
Size: 393 556 55 126 1611 366 144 12
Cuts at: 4502 4898 5015 5036 5225 5699 6602 7199 7986
Size: 396 117 21 189 474 903 597 787
Cuts at: 7986 8254 1239
Size: 268 1524
Fragments arranged by size:
1611 1524 903 787 597 556 474 396
393 366 268 189 144 126 117 55
21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2569 3491 5321 2569
Size: 922 1830 5787
Fragments arranged by size:
5787 1830 922
EcoO109I rG'GnC_Cy
Cuts at: 3613 4447 5277 5392 5407 3613
Size: 834 830 115 15 6745
Fragments arranged by size:
6745 834 830 115 15
EcoRI G'AATT_C
Cuts at: 3950 5848 6226 3950
Size: 1898 378 6263
Fragments arranged by size:
6263 1898 378
EcoRV GAT'ATC
Cuts at: 3634 3634
Size: 8539
FspI TGC'GCA
Cuts at: 7045 7045
Size: 8539
HaeII r_GCGC'y
Cuts at: 1678 3219 4101 5203 5475 7686 1678
Size: 1541 882 1102 272 2211 2531
Fragments arranged by size:
2531 2211 1541 1102 882 272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2307 2339 2447
Size: 32 135 32 449 32 254 32 108
Cuts at: 2447 2479 2587 2594 2619 2626 2860 2892 2956
Size: 32 108 7 25 7 234 32 64
Cuts at: 2956 2975 2988 3007 3618 3650 3825 3857 4439
Size: 19 13 19 611 32 175 32 582
Cuts at: 4439 4471 5169 5201 5206 5238 7180 7212 7254
Size: 32 698 32 5 32 1942 32 42
Cuts at: 7254 7286 1373
Size: 32 2626
Fragments arranged by size:
2626 1942 698 611 582 449 254 234
175 135 108 108 64 42 32 32
32 32 32 32 32 32 32 32
32 32 32 25 19 19 13 7
7 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2731 3966 5883 6129 669
Size: 988 281 793 1235 1917 246 3079
Fragments arranged by size:
3079 1917 1235 988 793 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8539
HpaI GTT'AAC
Cuts at: 3966 6129 3966
Size: 2163 6376
KpnI G_GTAC'C
Cuts at: 5864 5864
Size: 8539
MfeI C'AATT_G
Cuts at: 4359 6138 4359
Size: 1779 6760
MluI A'CGCG_T
Cuts at: 8415 8415
Size: 8539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2064 4224 7653 7837 1305
Size: 759 2160 3429 184 2007
Fragments arranged by size:
3429 2160 2007 759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 2951 5828 10
Size: 54 1425 343 816 303 2877 2721
Fragments arranged by size:
2877 2721 1425 816 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 2501 2738 3230 3998 4856 4974
Size: 648 1072 263 237 492 768 858 118
Cuts at: 4974 5439 5498 6497 6856 7015 518
Size: 465 59 999 359 159 2042
Fragments arranged by size:
2042 1072 999 858 768 648 492 465
359 263 237 159 118 59
MspA1I CmG'CkG
Cuts at: 1606 2268 2908 3296 4345 5014 5522 5955 6632
Size: 662 640 388 1049 669 508 433 677
Cuts at: 6632 7780 8025 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1049 677 669 662 640 508
433 388 245
NcoI C'CATG_G
Cuts at: 513 1065 3969 4224 513
Size: 552 2904 255 4828
Fragments arranged by size:
4828 2904 552 255
NdeI CA'TA_TG
Cuts at: 387 2787 4521 387
Size: 2400 1734 4405
Fragments arranged by size:
4405 2400 1734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8539
NotI GC'GGCC_GC
Cuts at: 5986 5986
Size: 8539
NruI TCG'CGA
Cuts at: 3165 5304 3165
Size: 2139 6400
NsiI A_TGCA'T
Cuts at: 2090 2158 2090
Size: 68 8471
NspI r_CATG'y
Cuts at: 1132 1229 2506 2938 4604 5792 5897 7442 1132
Size: 97 1277 432 1666 1188 105 1545 2229
Fragments arranged by size:
2229 1666 1545 1277 1188 432 105 97
PasI CC'CwG_GG
Cuts at: 1348 4985 1348
Size: 3637 4902
PciI A'CATG_T
Cuts at: 2502 7438 2502
Size: 4936 3603
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3226 4011 4033 4348 5824 1108
Size: 2118 785 22 315 1476 3823
Fragments arranged by size:
3823 2118 1476 785 315 22
PfoI T'CCnGG_A
Cuts at: 2514 2616 3060 3957 4032 4992 2514
Size: 102 444 897 75 960 6061
Fragments arranged by size:
6061 960 897 444 102 75
PmeI GTTT'AAAC
Cuts at: 5843 5843
Size: 8539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6608 6640 8121 8153 6608
Size: 32 1481 32 6994
Fragments arranged by size:
6994 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3613 4447 5277 5392 3613
Size: 834 830 115 6760
Fragments arranged by size:
6760 834 830 115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8539
PsiI TTA'TAA
Cuts at: 6109 6109
Size: 8539
PspOMI G'GGCC_C
Cuts at: 5407 5407
Size: 8539
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2747 2779 3425 3457 2747
Size: 32 646 32 7829
Fragments arranged by size:
7829 646 32 32
PstI C_TGCA'G
Cuts at: 830 2417 2913 3198 3387 3915 4832 5391 5891
Size: 1587 496 285 189 528 917 559 500
Cuts at: 5891 830
Size: 3478
Fragments arranged by size:
3478 1587 917 559 528 500 496 285
189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8539
PvuII CAG'CTG
Cuts at: 2268 2908 4345 5014 5522 2268
Size: 640 1437 669 508 5285
Fragments arranged by size:
5285 1437 669 640 508
SacI G_AGCT'C
Cuts at: 3093 3171 5043 3093
Size: 78 1872 6589
Fragments arranged by size:
6589 1872 78
SacII CC_GC'GG
Cuts at: 3297 3297
Size: 8539
SalI G'TCGA_C
Cuts at: 1936 5881 1936
Size: 3945 4594
SanDI GG'GwC_CC
Cuts at: 5277 5277
Size: 8539
SapI GCTCTTCn'nnn_
Cuts at: 2052 3176 4883 2052
Size: 1124 1707 5708
Fragments arranged by size:
5708 1707 1124
SbfI CC_TGCA'GG
Cuts at: 3387 5391 5891 3387
Size: 2004 500 6035
Fragments arranged by size:
6035 2004 500
ScaI AGT'ACT
Cuts at: 6787 8499 6787
Size: 1712 6827
SexAI A'CCwGG_T
Cuts at: 5250 5250
Size: 8539
SfcI C'TryA_G
Cuts at: 826 1045 2193 2413 2909 3100 3194 3383 3533
Size: 219 1148 220 496 191 94 189 150
Cuts at: 3533 3911 4768 4780 4789 4828 5387 5887 7022
Size: 378 857 12 9 39 559 500 1135
Cuts at: 7022 7703 7894 826
Size: 681 191 1471
Fragments arranged by size:
1471 1148 1135 857 681 559 500 496
378 220 219 191 191 189 150 94
39 12 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8539
SmaI CCC'GGG
Cuts at: 1872 2276 5866 1872
Size: 404 3590 4545
Fragments arranged by size:
4545 3590 404
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2795 3598 6643 7544 7806 820
Size: 197 926 852 803 3045 901 262 1553
Fragments arranged by size:
3045 1553 926 901 852 803 262 197
SnaBI TAC'GTA
Cuts at: 493 6330 493
Size: 5837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8539
SphI G_CATG'C
Cuts at: 1132 5897 1132
Size: 4765 3774
SspI AAT'ATT
Cuts at: 5 52 6463 5
Size: 47 6411 2081
Fragments arranged by size:
6411 2081 47
StuI AGG'CCT
Cuts at: 1517 2471 3381 3677 1517
Size: 954 910 296 6379
Fragments arranged by size:
6379 954 910 296
StyI C'CwwG_G
Cuts at: 513 1065 2485 3969 4224 4413 4900 5322 5823
Size: 552 1420 1484 255 189 487 422 501
Cuts at: 5823 5978 513
Size: 155 3074
Fragments arranged by size:
3074 1484 1420 552 501 487 422 255
189 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2681 3023 4706 5824 6566 6583 6736
Size: 1255 1101 342 1683 1118 742 17 153
Cuts at: 6736 6921 7366 325
Size: 185 445 1498
Fragments arranged by size:
1683 1498 1255 1118 1101 742 445 342
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 3030 3234
Size: 274 80 33 51 1443 276 777 204
Cuts at: 3234 3753 4086 5487 6785 8497 96
Size: 519 333 1401 1298 1712 138
Fragments arranged by size:
1712 1443 1401 1298 777 519 333 276
274 204 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3854 3886 4457 4489 4556 4588 5318 5350 8499
Size: 32 571 32 67 32 730 32 3149
Cuts at: 8499 8531 3854
Size: 32 3862
Fragments arranged by size:
3862 3149 730 571 67 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2459 2726 1316
Size: 1143 267 7129
Fragments arranged by size:
7129 1143 267
XbaI T'CTAG_A
Cuts at: 5875 5875
Size: 8539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4465 4465
Size: 8539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8539
XmaI C'CCGG_G
Cuts at: 1870 2274 5864 1870
Size: 404 3590 4545
Fragments arranged by size:
4545 3590 404
XmnI GAAnn'nnTTC
Cuts at: 2061 6668 2061
Size: 4607 3932
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6347 276
Size: 53 83 186 936 4813 2468
Fragments arranged by size:
4813 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV
PacI PmlI PspXI RsrII SfiI SfoI SrfI SwaI