Restriction map for
pFN21AA1161
(Circular) (Six-base) MAPSORT of: pFN21AA1161.seq Check: 9394 from: 1 to: 6745
pFN21AA1161 6745 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2044 2983 3252 2044
Size: 939 269 5537
Fragments arranged by size:
5537 939 269
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2769 3927 4555 278
Size: 53 83 186 936 1233 1158 628 2468
Fragments arranged by size:
2468 1233 1158 936 628 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2544 4008 4088 1937
Size: 607 1464 80 4594
Fragments arranged by size:
4594 1464 607 80
Acc65I G'GTAC_C
Cuts at: 4066 4066
Size: 6745
AclI AA'CG_TT
Cuts at: 1563 4872 5245 1563
Size: 3309 373 3063
Fragments arranged by size:
3309 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2660 3307 4808 6192 6460 1632
Size: 452 576 647 1501 1384 268 1917
Fragments arranged by size:
1917 1501 1384 647 576 452 268
AfeI AGC'GCT
Cuts at: 2582 2663 2582
Size: 81 6664
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6548
AflIII A'CryG_T
Cuts at: 1203 2964 3027 5644 6621 1203
Size: 1761 63 2617 977 1327
Fragments arranged by size:
2617 1761 1327 977 63
AgeI A'CCGG_T
Cuts at: 5576 5576
Size: 6745
AhdI GACnn_n'nnGTC
Cuts at: 5474 5474
Size: 6745
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6745
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3593 1184
Size: 2409 4336
AlwNI CAG_nnn'CTG
Cuts at: 3357 4488 6060 3357
Size: 1131 1572 4042
Fragments arranged by size:
4042 1572 1131
ApaLI G'TGCA_C
Cuts at: 3074 4802 5958 3074
Size: 1728 1156 3861
Fragments arranged by size:
3861 1728 1156
ApoI r'AATT_y
Cuts at: 1493 3008 4054 4280 4432 6541 1493
Size: 1515 1046 226 152 2109 1697
Fragments arranged by size:
2109 1697 1515 1046 226 152
AscI GG'CGCG_CC
Cuts at: 2878 2878
Size: 6745
AseI AT'TA_AT
Cuts at: 160 1032 5299 160
Size: 872 4267 1606
Fragments arranged by size:
4267 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
AvaI C'yCGr_G
Cuts at: 1870 1943 2576 2831 3608 4070 1870
Size: 73 633 255 777 462 4545
Fragments arranged by size:
4545 777 633 462 255 73
BamHI G'GATC_C
Cuts at: 4075 4442 4075
Size: 367 6378
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2288 2465 2821 3748 4066
Size: 325 217 627 501 177 356 927 318
Cuts at: 4066 5521 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 927 627 501 356 325 318
217 177
BanII G_rGCy'C
Cuts at: 1391 2081 2520 2762 3621 4064 1391
Size: 690 439 242 859 443 4072
Fragments arranged by size:
4072 859 690 443 439 242
BbeI G_GCGC'C
Cuts at: 2292 2469 2292
Size: 177 6568
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 928
Size: 971 325 5449
Fragments arranged by size:
5449 971 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4936 4970 1903
Size: 34 2999 34 3678
Fragments arranged by size:
3678 2999 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4637 5847 4637
Size: 1210 5535
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6745
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2286 2505 3734
Size: 107 122 71 631 605 614 219 1229
Cuts at: 3734 5356 136
Size: 1622 1525
Fragments arranged by size:
1622 1525 1229 631 614 605 219 122
107 71
BglII A'GATC_T
Cuts at: 6740 6740
Size: 6745
BlpI GC'TnA_GC
Cuts at: 2738 4162 2738
Size: 1424 5321
Bme1580I G_kGCm'C
Cuts at: 1790 2634 3078 3582 3609 4806 5962 1790
Size: 844 444 504 27 1197 1156 2573
Fragments arranged by size:
2573 1197 1156 844 504 444 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6745
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4476 5434 442
Size: 416 53 83 399 24 3059 958 1753
Fragments arranged by size:
3059 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3379 3920 5405 1239
Size: 2140 541 1485 2579
Fragments arranged by size:
2579 2140 1485 541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3782 4870 5735 6033 3782
Size: 1088 865 298 4494
Fragments arranged by size:
4494 1088 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2838 5408 882
Size: 617 1339 2570 2219
Fragments arranged by size:
2570 2219 1339 617
BsaAI yAC'GTr
Cuts at: 493 1204 4536 493
Size: 711 3332 2702
Fragments arranged by size:
3332 2702 711
BsaBI GATnn'nnATC
Cuts at: 6739 6739
Size: 6745
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2289 2466 2556 2766
Size: 53 83 186 936 756 177 90 210
Cuts at: 2766 3048 3924 3980 4040 4552 4934 5569 275
Size: 282 876 56 60 512 382 635 1451
Fragments arranged by size:
1451 936 876 756 635 512 382 282
210 186 177 90 83 60 56 53
BsaWI w'CCGG_w
Cuts at: 5178 5576 5850 5997 6408 5178
Size: 398 274 147 411 5515
Fragments arranged by size:
5515 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2126 2156 2214 2244 2255
Size: 30 165 30 723 30 58 30 11
Cuts at: 2255 2285 2756 2786 1178
Size: 30 471 30 5137
Fragments arranged by size:
5137 723 471 165 58 30 30 30
30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2408 2539 1186
Size: 1222 131 5392
Fragments arranged by size:
5392 1222 131
BseYI C'CCAG_C
Cuts at: 2074 2296 2388 2520 3350 3532 3582 5948 6444
Size: 222 92 132 830 182 50 2366 496
Cuts at: 6444 2074
Size: 2375
Fragments arranged by size:
2375 2366 830 496 222 182 132 92
50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 2912 1163
Size: 573 579 597 4996
Fragments arranged by size:
4996 597 579 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2316 2607 2719 3338 4008 4195
Size: 275 186 315 291 112 619 670 187
Cuts at: 4195 4956 5984 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 670 619 315 291 275
187 186 112
BsiHKAI G_wGCw'C
Cuts at: 2424 3078 3519 3621 4806 4891 5962 2424
Size: 654 441 102 1185 85 1071 3207
Fragments arranged by size:
3207 1185 1071 654 441 102 85
BsmI GAATG_Cn'
Cuts at: 4256 4349 4256
Size: 93 6652
BsmBI CGTCTCn'nnnn_
Cuts at: 2146 2146
Size: 6745
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2424 2520 2634 2762 3078 3519
Size: 399 291 343 96 114 128 316 441
Cuts at: 3519 3582 3609 3621 4064 4806 4891 5962 1391
Size: 63 27 12 443 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 441 399 343 316
291 128 114 96 85 63 27 12
BspEI T'CCGG_A
Cuts at: 6408 6408
Size: 6745
BspHI T'CATG_A
Cuts at: 4634 6364 4634
Size: 1730 5015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2044 2218 2983 3169 3252 3318 4100 844
Size: 1200 174 765 186 83 66 782 3489
Fragments arranged by size:
3489 1200 782 765 186 174 83 66
BsrBI CCG'CTC
Cuts at: 1394 1823 2250 4632 1394
Size: 429 427 2382 3507
Fragments arranged by size:
3507 2382 429 427
BsrDI GCAATG_nn'
Cuts at: 65 1248 5240 5414 65
Size: 1183 3992 174 1396
Fragments arranged by size:
3992 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2714 3050 3153 3333 3363 3593 3655
Size: 110 904 336 103 180 30 230 62
Cuts at: 3655 3938 3982 5389 5576 1700
Size: 283 44 1407 187 2869
Fragments arranged by size:
2869 1407 904 336 283 230 187 180
110 103 62 44 30
BsrGI T'GTAC_A
Cuts at: 96 2007 2967 96
Size: 1911 960 3874
Fragments arranged by size:
3874 1911 960
BssHII G'CGCG_C
Cuts at: 1925 2256 2599 2681 2878 1925
Size: 331 343 82 197 5792
Fragments arranged by size:
5792 343 331 197 82
BssSI C'ACGA_G
Cuts at: 4805 5817 4805
Size: 1012 5733
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6745
BstBI TT'CG_AA
Cuts at: 5549 5549
Size: 6745
BstEII G'GTnAC_C
Cuts at: 1184 3156 3778 3791 1184
Size: 1972 622 13 4138
Fragments arranged by size:
4138 1972 622 13
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2459 2500 2434
Size: 25 41 6679
Fragments arranged by size:
6679 41 25
BstYI r'GATC_y
Cuts at: 1972 2919 4075 4442 4827 4844 6296 6382 6394
Size: 947 1156 367 385 17 1452 86 12
Cuts at: 6394 6740 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1156 947 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2082 2112 2415 2883 3147 3270 3314
Size: 552 1017 30 303 468 264 123 44
Cuts at: 3314 3510 513
Size: 196 3748
Fragments arranged by size:
3748 1017 552 468 303 264 196 123
44 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2298 2553 3615 3694 487
Size: 683 56 1072 255 1062 79 3538
Fragments arranged by size:
3538 1072 1062 683 255 79 56
BtsI GCAGTG_nn'
Cuts at: 4261 5055 5075 4261
Size: 794 20 5931
Fragments arranged by size:
5931 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6745
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6710
DraI TTT'AAA
Cuts at: 4049 4049
Size: 6745
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3353 5752 809
Size: 507 2037 2399 1802
Fragments arranged by size:
2399 2037 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2313 2506
Size: 54 1425 50 275 18 279 204 193
Cuts at: 2506 2604 2716 3335 3642 3657 3705 4192 5081
Size: 98 112 619 307 15 48 487 889
Cuts at: 5081 6710 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 619 487 307 279 275
204 193 112 98 54 50 48 43
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2313 2604 2716 3335 4192 1537
Size: 275 501 291 112 619 857 4090
Fragments arranged by size:
4090 857 619 501 291 275 112
EarI CTCTTCn'nnn_
Cuts at: 1353 4675 1353
Size: 3322 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2171 2225 2486 2912 3023 3419
Size: 1064 138 582 54 261 426 111 396
Cuts at: 3419 3440 5332 5706 5852 387
Size: 21 1892 374 146 1280
Fragments arranged by size:
1892 1280 1064 582 426 396 374 261
146 138 111 54 21
EcoICRI GAG'CTC
Cuts at: 3619 3619
Size: 6745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2660 3307 3379 3920 4808 5405
Size: 393 452 576 647 72 541 888 597
Cuts at: 5405 6192 6460 1239
Size: 787 268 1524
Fragments arranged by size:
1524 888 787 647 597 576 541 452
393 268 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 2150
Size: 6745
EcoRI G'AATT_C
Cuts at: 4054 4432 4054
Size: 378 6367
FspI TGC'GCA
Cuts at: 5251 5251
Size: 6745
HaeII r_GCGC'y
Cuts at: 1678 2292 2405 2469 2584 2665 2732 2870 3370
Size: 614 113 64 115 81 67 138 500
Cuts at: 3370 3386 3688 5892 1678
Size: 16 302 2204 2531
Fragments arranged by size:
2531 2204 614 500 302 138 115 113
81 67 64 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2214 2246 2255
Size: 32 135 32 443 32 167 32 9
Cuts at: 2255 2287 2531 2563 2756 2788 3452 3484 5386
Size: 32 244 32 193 32 664 32 1902
Cuts at: 5386 5418 5460 5492 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1902 664 443 244 193 167 135
42 32 32 32 32 32 32 32
32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3088 4009 4089 4335 669
Size: 988 281 1150 921 80 246 3079
Fragments arranged by size:
3079 1150 988 921 281 246 80
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6745
HpaI GTT'AAC
Cuts at: 4335 4335
Size: 6745
KasI G'GCGC_C
Cuts at: 2288 2465 2288
Size: 177 6568
KpnI G_GTAC'C
Cuts at: 4070 4070
Size: 6745
MfeI C'AATT_G
Cuts at: 4344 4344
Size: 6745
MluI A'CGCG_T
Cuts at: 6621 6621
Size: 6745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2577 5859 6043 1305
Size: 1272 3282 184 2007
Fragments arranged by size:
3282 2007 1272 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2508 3707 10
Size: 54 1425 343 676 1199 3048
Fragments arranged by size:
3048 1425 1199 676 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 2457 3377 4703 5062 5221 518
Size: 648 1203 88 920 1326 359 159 2042
Fragments arranged by size:
2042 1326 1203 920 648 359 159 88
MspA1I CmG'CkG
Cuts at: 1606 2071 2084 2114 2329 3316 3340 3512 3586
Size: 465 13 30 215 987 24 172 74
Cuts at: 3586 3754 4161 4838 5986 6231 1606
Size: 168 407 677 1148 245 2120
Fragments arranged by size:
2120 1148 987 677 465 407 245 215
172 168 74 30 24 13
NaeI GCC'GGC
Cuts at: 2716 3052 3365 3595 3657 3940 2716
Size: 336 313 230 62 283 5521
Fragments arranged by size:
5521 336 313 283 230 62
NarI GG'CG_CC
Cuts at: 2289 2466 2289
Size: 177 6568
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6193
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6745
NgoMIV G'CCGG_C
Cuts at: 2714 3050 3363 3593 3655 3938 2714
Size: 336 313 230 62 283 5521
Fragments arranged by size:
5521 336 313 283 230 62
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6745
NotI GC'GGCC_GC
Cuts at: 2604 4192 2604
Size: 1588 5157
NruI TCG'CGA
Cuts at: 3465 3465
Size: 6745
NspI r_CATG'y
Cuts at: 1132 1229 2968 3031 3532 4103 5648 1132
Size: 97 1739 63 501 571 1545 2229
Fragments arranged by size:
2229 1739 1545 571 501 97 63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6745
PciI A'CATG_T
Cuts at: 2964 3027 5644 2964
Size: 63 2617 4065
Fragments arranged by size:
4065 2617 63
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3357 1108
Size: 2249 4496
PmeI GTTT'AAAC
Cuts at: 4049 4049
Size: 6745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4814 4846 6327 6359 4814
Size: 32 1481 32 5200
Fragments arranged by size:
5200 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6745
PsiI TTA'TAA
Cuts at: 4315 4315
Size: 6745
PspXI vC'TCGA_Gb
Cuts at: 2576 2576
Size: 6745
PstI C_TGCA'G
Cuts at: 830 2279 4097 830
Size: 1449 1818 3478
Fragments arranged by size:
3478 1818 1449
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6745
SacI G_AGCT'C
Cuts at: 3621 3621
Size: 6745
SacII CC_GC'GG
Cuts at: 2085 2115 3317 3513 2085
Size: 30 1202 196 5317
Fragments arranged by size:
5317 1202 196 30
SalI G'TCGA_C
Cuts at: 1936 4007 4087 1936
Size: 2071 80 4594
Fragments arranged by size:
4594 2071 80
SbfI CC_TGCA'GG
Cuts at: 4097 4097
Size: 6745
ScaI AGT'ACT
Cuts at: 4993 6705 4993
Size: 1712 5033
SfcI C'TryA_G
Cuts at: 826 1045 2134 2275 4093 5228 5909 6100 826
Size: 219 1089 141 1818 1135 681 191 1471
Fragments arranged by size:
1818 1471 1135 1089 681 219 191 141
SfoI GGC'GCC
Cuts at: 2290 2467 2290
Size: 177 6568
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
SgrAI Cr'CCGG_yG
Cuts at: 1700 3982 1700
Size: 2282 4463
SmaI CCC'GGG
Cuts at: 1872 4072 1872
Size: 2200 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2576 3761 4849 5750 6012 820
Size: 197 926 633 1185 1088 901 262 1553
Fragments arranged by size:
1553 1185 1088 926 901 633 262 197
SnaBI TAC'GTA
Cuts at: 493 4536 493
Size: 4043 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6745
SphI G_CATG'C
Cuts at: 1132 3532 4103 1132
Size: 2400 571 3774
Fragments arranged by size:
3774 2400 571
SspI AAT'ATT
Cuts at: 5 52 4669 5
Size: 47 4617 2081
Fragments arranged by size:
4617 2081 47
StuI AGG'CCT
Cuts at: 1517 2837 1517
Size: 1320 5425
StyI C'CwwG_G
Cuts at: 513 1065 2358 4184 513
Size: 552 1293 1826 3074
Fragments arranged by size:
3074 1826 1293 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3061 3228 4772 4789 4942 5127 5572
Size: 1255 1481 167 1544 17 153 185 445
Cuts at: 5572 325
Size: 1498
Fragments arranged by size:
1544 1498 1481 1255 445 185 167 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2784 2967
Size: 274 80 33 51 1443 30 777 183
Cuts at: 2967 4991 6703 96
Size: 2024 1712 138
Fragments arranged by size:
2024 1712 1443 777 274 183 138 80
51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6705 6737 6705
Size: 32 6713
Tth111I GACn'n_nGTC
Cuts at: 1316 2366 1316
Size: 1050 5695
XbaI T'CTAG_A
Cuts at: 4081 4081
Size: 6745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2459 2459
Size: 6745
XhoI C'TCGA_G
Cuts at: 1943 2576 1943
Size: 633 6112
XmaI C'CCGG_G
Cuts at: 1870 4070 1870
Size: 2200 4545
XmnI GAAnn'nnTTC
Cuts at: 4874 4874
Size: 6745
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2767 3925 4553 276
Size: 53 83 186 936 1233 1158 628 2468
Fragments arranged by size:
2468 1233 1158 936 628 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI
FspAI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI