Restriction map for
pFN21AA1150
(Circular) (Six-base) MAPSORT of: pFN21AA1150.seq Check: 6134 from: 1 to: 6484
pFN21AA1150 6484 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4547 5931 6199 1632
Size: 2915 1384 268 1917
Fragments arranged by size:
2915 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 2778 2794 5383 6360 1203
Size: 1575 16 2589 977 1327
Fragments arranged by size:
2589 1575 1327 977 16
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6484
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 6452
AlwNI CAG_nnn'CTG
Cuts at: 4227 5799 4227
Size: 1572 4912
ApaI G_GGCC'C
Cuts at: 2698 2840 2698
Size: 142 6342
ApaLI G'TGCA_C
Cuts at: 4541 5697 4541
Size: 1156 5328
ApoI r'AATT_y
Cuts at: 1493 2716 3793 4019 4171 6280 1493
Size: 1223 1077 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 1077 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 3809 1870
Size: 73 1866 4545
Fragments arranged by size:
4545 1866 73
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 3610 3805 5260 618
Size: 325 217 627 392 1431 195 1455 1842
Fragments arranged by size:
1842 1455 1431 627 392 325 217 195
BanII G_rGCy'C
Cuts at: 1391 2698 2840 3803 1391
Size: 1307 142 963 4072
Fragments arranged by size:
4072 1307 963 142
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2606 928
Size: 971 707 4806
Fragments arranged by size:
4806 971 707
BbvCI CC'TCA_GC
Cuts at: 2510 2510
Size: 6484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4675 4709 1903
Size: 34 2738 34 3678
Fragments arranged by size:
3678 2738 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BfrBI ATG'CAT
Cuts at: 3395 3395
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3065 5095 136
Size: 107 122 71 631 605 1393 2030 1525
Fragments arranged by size:
2030 1525 1393 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 3901 3901
Size: 6484
Bme1580I G_kGCm'C
Cuts at: 1790 2698 2840 3268 4545 5701 1790
Size: 908 142 428 1277 1156 2573
Fragments arranged by size:
2573 1277 1156 908 428 142
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3262 3610 3696
Size: 416 53 83 399 24 1845 348 86
Cuts at: 3696 4215 5173 442
Size: 519 958 1753
Fragments arranged by size:
1845 1753 958 519 416 399 348 86
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2589 3119 3459 3542 5144 1239
Size: 1350 530 340 83 1602 2579
Fragments arranged by size:
2579 1602 1350 530 340 83
Bpu10I CC'TnA_GC
Cuts at: 2510 2844 2510
Size: 334 6150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 4609 5474 5772 2195
Size: 2414 865 298 2907
Fragments arranged by size:
2907 2414 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3497 5147 882
Size: 617 1998 1650 2219
Fragments arranged by size:
2219 1998 1650 617
BsaAI yAC'GTr
Cuts at: 493 1204 4275 493
Size: 711 3071 2702
Fragments arranged by size:
3071 2702 711
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4291 4673 5308 275
Size: 53 83 186 936 2758 382 635 1451
Fragments arranged by size:
2758 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4917 5315 5589 5736 6147 4917
Size: 398 274 147 411 5254
Fragments arranged by size:
5254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3457 3487 1178
Size: 30 165 30 2054 30 4175
Fragments arranged by size:
4175 2054 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 1186
Size: 1312 5172
BseYI C'CCAG_C
Cuts at: 2062 2812 2817 3020 5687 6183 2062
Size: 750 5 203 2667 496 2363
Fragments arranged by size:
2667 2363 750 496 203 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3171 1163
Size: 573 1435 4476
Fragments arranged by size:
4476 1435 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3934 4695 5723 1540
Size: 275 186 1933 761 1028 2301
Fragments arranged by size:
2301 1933 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4545 4630 5701 4545
Size: 85 1071 5328
Fragments arranged by size:
5328 1071 85
BsmI GAATG_Cn'
Cuts at: 2623 3995 4088 2623
Size: 1372 93 5019
Fragments arranged by size:
5019 1372 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2698 2840 3268 3803 4545 4630 5701
Size: 399 908 142 428 535 742 85 1071
Cuts at: 5701 1391
Size: 2174
Fragments arranged by size:
2174 1071 908 742 535 428 399 142
85
BspEI T'CCGG_A
Cuts at: 6147 6147
Size: 6484
BspHI T'CATG_A
Cuts at: 3510 4373 6103 3510
Size: 863 1730 3891
Fragments arranged by size:
3891 1730 863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3839 844
Size: 2995 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2488 3585 4371 1394
Size: 429 665 1097 786 3507
Fragments arranged by size:
3507 1097 786 665 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2144 3660 4979 5153 65
Size: 1183 896 1516 1319 174 1396
Fragments arranged by size:
1516 1396 1319 1183 896 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3381 5128 5315 1700
Size: 110 1571 1747 187 2869
Fragments arranged by size:
2869 1747 1571 187 110
BsrGI T'GTAC_A
Cuts at: 96 2611 96
Size: 2515 3969
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 4544 5556 4544
Size: 1012 5472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 3102 3814 4181 4566 4583 6035 6121 6133
Size: 1130 712 367 385 17 1452 86 12
Cuts at: 6133 6479 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1130 712 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2121 513
Size: 552 939 117 4876
Fragments arranged by size:
4876 939 552 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5745
Fragments arranged by size:
5745 683 56
BtsI GCAGTG_nn'
Cuts at: 2218 3338 4000 4794 4814 2218
Size: 1120 662 794 20 3888
Fragments arranged by size:
3888 1120 794 662 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2763 2798 529
Size: 35 2199 35 4215
Fragments arranged by size:
4215 2199 35 35
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3931 4820 6449
Size: 54 1425 50 275 18 2101 889 1629
Cuts at: 6449 8
Size: 43
Fragments arranged by size:
2101 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3931 1537
Size: 275 2119 4090
Fragments arranged by size:
4090 2119 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2034 3075 4414 1353
Size: 681 1041 1339 3423
Fragments arranged by size:
3423 1339 1041 681
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5071 5445 5591 387
Size: 1064 138 3482 374 146 1280
Fragments arranged by size:
3482 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2589 3119 3459 3542 4547 5144 5931
Size: 393 957 530 340 83 1005 597 787
Cuts at: 5931 6199 1239
Size: 268 1524
Fragments arranged by size:
1524 1005 957 787 597 530 393 340
268 83
EcoNI CCTnn'n_nnAGG
Cuts at: 3413 3413
Size: 6484
EcoO109I rG'GnC_Cy
Cuts at: 2665 2694 2836 3698 2665
Size: 29 142 862 5451
Fragments arranged by size:
5451 862 142 29
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2045 2071 2255 2045
Size: 26 184 6274
Fragments arranged by size:
6274 184 26
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 3027 5631 1678
Size: 1349 2604 2531
Fragments arranged by size:
2604 2531 1349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 2554 2586 5125
Size: 32 135 32 495 32 455 32 2539
Cuts at: 5125 5157 5199 5231 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2539 495 455 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3658 3828 4074 669
Size: 988 281 1720 170 246 3079
Fragments arranged by size:
3079 1720 988 281 246 170
HindIII A'AGCT_T
Cuts at: 748 2675 748
Size: 1927 4557
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3369 5598 5782 1305
Size: 2064 2229 184 2007
Fragments arranged by size:
2229 2064 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4662
Fragments arranged by size:
4662 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2783 2954 3173 4442 4801 4960 518
Size: 648 1617 171 219 1269 359 159 2042
Fragments arranged by size:
2042 1617 1269 648 359 219 171 159
MspA1I CmG'CkG
Cuts at: 1606 2509 2833 3082 3479 3900 4577 5725 5970
Size: 903 324 249 397 421 677 1148 245
Cuts at: 5970 1606
Size: 2120
Fragments arranged by size:
2120 1148 903 677 421 397 324 249
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2121 513
Size: 552 939 117 4876
Fragments arranged by size:
4876 939 552 117
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NsiI A_TGCA'T
Cuts at: 3397 3397
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 2782 3842 5387 1132
Size: 97 1553 1060 1545 2229
Fragments arranged by size:
2229 1553 1545 1060 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 2778 5383 2778
Size: 2605 3879
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6484
PfoI T'CCnGG_A
Cuts at: 3096 3096
Size: 6484
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PspOMI G'GGCC_C
Cuts at: 2694 2836 2694
Size: 142 6342
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3423 3455 3423
Size: 32 6452
PstI C_TGCA'G
Cuts at: 830 3058 3836 830
Size: 2228 778 3478
Fragments arranged by size:
3478 2228 778
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2509 3082 3479 2509
Size: 573 397 5514
Fragments arranged by size:
5514 573 397
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SapI GCTCTTCn'nnn_
Cuts at: 2034 3075 2034
Size: 1041 5443
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SfcI C'TryA_G
Cuts at: 826 1045 2576 2822 3054 3410 3832 4967 5648
Size: 219 1531 246 232 356 422 1135 681
Cuts at: 5648 5839 826
Size: 191 1471
Fragments arranged by size:
1531 1471 1135 681 422 356 246 232
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3811 1872
Size: 1939 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 4588 5489 5751 820
Size: 197 926 231 2414 901 262 1553
Fragments arranged by size:
2414 1553 926 901 262 231 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3842 1132
Size: 2710 3774
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 2257 1517
Size: 740 5744
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2698 3217 3640 3923 513
Size: 552 939 117 577 519 423 283 3074
Fragments arranged by size:
3074 939 577 552 519 423 283 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2887 3729 4511 4528 4681 4866 5311
Size: 1255 1307 842 782 17 153 185 445
Cuts at: 5311 325
Size: 1498
Fragments arranged by size:
1498 1307 1255 842 782 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2611 4730 6442
Size: 274 80 33 51 1443 634 2119 1712
Cuts at: 6442 96
Size: 138
Fragments arranged by size:
2119 1712 1443 634 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3457 3489 6444 6476 3457
Size: 32 2955 32 3465
Fragments arranged by size:
3465 2955 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2568 1316
Size: 1252 5232
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2425 2425
Size: 6484
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 3809 1870
Size: 1939 4545
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspXI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI