Restriction map for
pFN21AA1147
(Circular) (Six-base) MAPSORT of: pFN21AA1147.seq Check: 1001 from: 1 to: 6070
pFN21AA1147 6070 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3051 3051
Size: 6070
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3003 3880 278
Size: 53 83 186 936 1467 877 2468
Fragments arranged by size:
2468 1467 936 877 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2640 3003 3413 1937
Size: 703 363 410 4594
Fragments arranged by size:
4594 703 410 363
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 2850 4197 4570 1563
Size: 1287 1347 373 3063
Fragments arranged by size:
3063 1347 1287 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3077 4133 5517 5785 1632
Size: 1445 1056 1384 268 1917
Fragments arranged by size:
1917 1445 1384 1056 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 2667 4969 5946 1203
Size: 1464 2302 977 1327
Fragments arranged by size:
2302 1464 1327 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 4799 4799
Size: 6070
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2645 1184
Size: 1461 4609
AlwNI CAG_nnn'CTG
Cuts at: 3052 3115 3813 5385 3052
Size: 63 698 1572 3737
Fragments arranged by size:
3737 1572 698 63
ApaI G_GGCC'C
Cuts at: 2054 2391 2054
Size: 337 5733
ApaLI G'TGCA_C
Cuts at: 4127 5283 4127
Size: 1156 4914
ApoI r'AATT_y
Cuts at: 1493 2756 3379 3605 3757 5866 1493
Size: 1263 623 226 152 2109 1697
Fragments arranged by size:
2109 1697 1263 623 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2159 2195 2269 3264 3395 1870
Size: 73 216 36 74 995 131 4545
Fragments arranged by size:
4545 995 216 131 74 73 36
BamHI G'GATC_C
Cuts at: 3400 3767 3400
Size: 367 5703
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2120 2199 2532 2873
Size: 325 217 627 239 94 79 333 341
Cuts at: 2873 3391 4846 618
Size: 518 1455 1842
Fragments arranged by size:
1842 1455 627 518 341 333 325 239
217 94 79
BanII G_rGCy'C
Cuts at: 1391 2054 2160 2391 3389 1391
Size: 663 106 231 998 4072
Fragments arranged by size:
4072 998 663 231 106
BbeI G_GCGC'C
Cuts at: 2030 2124 2203 2030
Size: 94 79 5897
Fragments arranged by size:
5897 94 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3032 3147 928
Size: 971 1133 115 3851
Fragments arranged by size:
3851 1133 971 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4261 4295 1903
Size: 34 2324 34 3678
Fragments arranged by size:
3678 2324 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6070
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2109 2129 2149
Size: 107 122 71 631 605 437 20 20
Cuts at: 2149 4681 136
Size: 2532 1525
Fragments arranged by size:
2532 1525 631 605 437 122 107 71
20 20
BglII A'GATC_T
Cuts at: 6065 6065
Size: 6070
BlpI GC'TnA_GC
Cuts at: 3487 3487
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2391 2876 4131 5287 1790
Size: 264 337 485 1255 1156 2573
Fragments arranged by size:
2573 1255 1156 485 337 264
BmgBI CAC'GTC
Cuts at: 1318 2218 1318
Size: 900 5170
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3801 4759 442
Size: 416 53 83 399 24 2384 958 1753
Fragments arranged by size:
2384 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2951 3338 4730 1239
Size: 986 726 387 1392 2579
Fragments arranged by size:
2579 1392 986 726 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2283 4195 5060 5358 2283
Size: 1912 865 298 2995
Fragments arranged by size:
2995 1912 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3285 4733 882
Size: 617 1786 1448 2219
Fragments arranged by size:
2219 1786 1448 617
BsaAI yAC'GTr
Cuts at: 493 1204 2905 3007 3861 493
Size: 711 1701 102 854 2702
Fragments arranged by size:
2702 1701 854 711 102
BsaBI GATnn'nnATC
Cuts at: 2684 2744 6064 2684
Size: 60 3320 2690
Fragments arranged by size:
3320 2690 60
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2121 2200 3000
Size: 53 83 186 936 494 94 79 800
Cuts at: 3000 3877 4259 4894 275
Size: 877 382 635 1451
Fragments arranged by size:
1451 936 877 800 635 494 382 186
94 83 79 53
BsaWI w'CCGG_w
Cuts at: 3198 3294 4503 4901 5175 5322 5733 3198
Size: 96 1209 398 274 147 411 3535
Fragments arranged by size:
3535 1209 411 398 274 147 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5845
Fragments arranged by size:
5845 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2237 2993 1186
Size: 991 60 756 4263
Fragments arranged by size:
4263 991 756 60
BseYI C'CCAG_C
Cuts at: 2040 2097 2209 2695 5273 5769 2040
Size: 57 112 486 2578 496 2341
Fragments arranged by size:
2578 2341 496 486 112 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2941 1163
Size: 573 1205 4292
Fragments arranged by size:
4292 1205 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2129 2133 2172 3520 4281 5309
Size: 275 186 128 4 39 1348 761 1028
Cuts at: 5309 1540
Size: 2301
Fragments arranged by size:
2301 1348 1028 761 275 186 128 39
4
BsiHKAI G_wGCw'C
Cuts at: 2601 4131 4216 5287 2601
Size: 1530 85 1071 3384
Fragments arranged by size:
3384 1530 1071 85
BsmI GAATG_Cn'
Cuts at: 3581 3674 3581
Size: 93 5977
BsmBI CGTCTCn'nnnn_
Cuts at: 2013 2065 2013
Size: 52 6018
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2160 2391 2601 2876 3389 4131
Size: 399 264 106 231 210 275 513 742
Cuts at: 4131 4216 5287 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 513 399 275 264 231
210 106 85
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 3321 3425 844
Size: 2207 270 104 3489
Fragments arranged by size:
3489 2207 270 104
BsrBI CCG'CTC
Cuts at: 1394 1823 2268 3957 1394
Size: 429 445 1689 3507
Fragments arranged by size:
3507 1689 445 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4565 4739 65
Size: 1183 3317 174 1396
Fragments arranged by size:
3317 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2128 2167 3090 4714 4901 1700
Size: 110 318 39 923 1624 187 2869
Fragments arranged by size:
2869 1624 923 318 187 110 39
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 2448 1925
Size: 523 5547
BssSI C'ACGA_G
Cuts at: 4130 5142 4130
Size: 1012 5058
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3052 3260 794
Size: 2258 208 3604
Fragments arranged by size:
3604 2258 208
BstBI TT'CG_AA
Cuts at: 2770 4874 2770
Size: 2104 3966
BstEII G'GTnAC_C
Cuts at: 1184 2251 1184
Size: 1067 5003
BstYI r'GATC_y
Cuts at: 1972 2739 3400 3767 4152 4169 5621 5707 5719
Size: 767 661 367 385 17 1452 86 12
Cuts at: 5719 6065 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 767 661 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2133 2424 513
Size: 552 939 129 291 4159
Fragments arranged by size:
4159 939 552 291 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 2489 2855 3586 4380 4400 2489
Size: 366 731 794 20 4159
Fragments arranged by size:
4159 794 731 366 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 3374 3374
Size: 6070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5077 809
Size: 507 3761 1802
Fragments arranged by size:
3761 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2126 2130 2169
Size: 54 1425 50 275 18 296 4 39
Cuts at: 2169 2238 2337 2844 3517 4406 6035 8
Size: 69 99 507 673 889 1629 43
Fragments arranged by size:
1629 1425 889 673 507 296 275 99
69 54 50 43 39 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2126 2130 2169 3517 1537
Size: 275 314 4 39 1348 4090
Fragments arranged by size:
4090 1348 314 275 39 4
EarI CTCTTCn'nnn_
Cuts at: 1353 3153 3173 4000 1353
Size: 1800 20 827 3423
Fragments arranged by size:
3423 1800 827 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4657 5031 5177 387
Size: 1064 138 3068 374 146 1280
Fragments arranged by size:
3068 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2951 3077 3338 4133 4730 5517
Size: 393 593 726 126 261 795 597 787
Cuts at: 5517 5785 1239
Size: 268 1524
Fragments arranged by size:
1524 795 787 726 597 593 393 268
261 126
EcoNI CCTnn'n_nnAGG
Cuts at: 2069 3317 2069
Size: 1248 4822
EcoO109I rG'GnC_Cy
Cuts at: 2050 2051 2074 2346 2387 2388 3163 2050
Size: 1 23 272 41 1 775 4957
Fragments arranged by size:
4957 775 272 41 23 1 1
EcoRI G'AATT_C
Cuts at: 3379 3757 3379
Size: 378 5692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 6070
FseI GG_CCGG'CC
Cuts at: 2132 2132
Size: 6070
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 2030 2124 2203 5217 1678
Size: 352 94 79 3014 2531
Fragments arranged by size:
3014 2531 352 94 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2315 2347 2724
Size: 32 135 32 680 32 31 32 377
Cuts at: 2724 2756 2776 2808 4711 4743 4785 4817 1373
Size: 32 20 32 1903 32 42 32 2626
Fragments arranged by size:
2626 1903 680 377 135 42 32 32
32 32 32 32 32 32 31 20
HincII GTy'rAC
Cuts at: 669 1657 1938 3414 3660 669
Size: 988 281 1476 246 3079
Fragments arranged by size:
3079 1476 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6070
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KasI G'GCGC_C
Cuts at: 2026 2120 2199 2026
Size: 94 79 5897
Fragments arranged by size:
5897 94 79
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2520 3198 5184 5368 1305
Size: 1215 678 1986 184 2007
Fragments arranged by size:
2007 1986 1215 678 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2339 2846 10
Size: 54 1425 343 507 507 3234
Fragments arranged by size:
3234 1425 507 507 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 4028 4387 4546 518
Size: 648 1257 1605 359 159 2042
Fragments arranged by size:
2042 1605 1257 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2135 2699 3052 3486 4163 5311 5556
Size: 426 103 564 353 434 677 1148 245
Cuts at: 5556 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 564 434 426 353 245
103
NaeI GCC'GGC
Cuts at: 2130 2169 3092 2130
Size: 39 923 5108
Fragments arranged by size:
5108 923 39
NarI GG'CG_CC
Cuts at: 2027 2121 2200 2027
Size: 94 79 5897
Fragments arranged by size:
5897 94 79
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4579
Fragments arranged by size:
4579 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6070
NgoMIV G'CCGG_C
Cuts at: 2128 2167 3090 2128
Size: 39 923 5108
Fragments arranged by size:
5108 923 39
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 2422 2509 2671 2710 3263 3428 4973
Size: 97 1193 87 162 39 553 165 1545
Cuts at: 4973 1132
Size: 2229
Fragments arranged by size:
2229 1545 1193 553 165 162 97 87
39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6070
PciI A'CATG_T
Cuts at: 2667 4969 2667
Size: 2302 3768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2236 1108
Size: 1128 4942
PfoI T'CCnGG_A
Cuts at: 3306 3306
Size: 6070
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2727 2759 4139 4171 5652 5684 2727
Size: 32 1380 32 1481 32 3113
Fragments arranged by size:
3113 1481 1380 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2346 3163 2346
Size: 817 5253
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6070
PsiI TTA'TAA
Cuts at: 2720 3640 2720
Size: 920 5150
PspOMI G'GGCC_C
Cuts at: 2050 2387 2050
Size: 337 5733
PstI C_TGCA'G
Cuts at: 830 2193 3048 3422 830
Size: 1363 855 374 3478
Fragments arranged by size:
3478 1363 855 374
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
PvuII CAG'CTG
Cuts at: 2699 2699
Size: 6070
SacII CC_GC'GG
Cuts at: 2136 2136
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SanDI GG'GwC_CC
Cuts at: 2346 2346
Size: 6070
SbfI CC_TGCA'GG
Cuts at: 3422 3422
Size: 6070
ScaI AGT'ACT
Cuts at: 4318 6030 4318
Size: 1712 4358
SexAI A'CCwGG_T
Cuts at: 2229 2523 2229
Size: 294 5776
SfcI C'TryA_G
Cuts at: 826 1045 2189 2818 3044 3418 4553 5234 5425
Size: 219 1144 629 226 374 1135 681 191
Cuts at: 5425 826
Size: 1471
Fragments arranged by size:
1471 1144 1135 681 629 374 226 219
191
SfoI GGC'GCC
Cuts at: 2028 2122 2201 2028
Size: 94 79 5897
Fragments arranged by size:
5897 94 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 2197 3266 3397 1872
Size: 325 1069 131 4545
Fragments arranged by size:
4545 1069 325 131
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2298 4174 5075 5337 820
Size: 197 926 355 1876 901 262 1553
Fragments arranged by size:
1876 1553 926 901 355 262 197
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3263 3428 1132
Size: 2131 165 3774
Fragments arranged by size:
3774 2131 165
SrfI GCCC'GGGC
Cuts at: 2197 2197
Size: 6070
SspI AAT'ATT
Cuts at: 5 52 3994 5
Size: 47 3942 2081
Fragments arranged by size:
3942 2081 47
StuI AGG'CCT
Cuts at: 1517 3323 1517
Size: 1806 4264
StyI C'CwwG_G
Cuts at: 513 1065 2004 3286 3509 513
Size: 552 939 1282 223 3074
Fragments arranged by size:
3074 1282 939 552 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2709 4097 4114 4267 4452 4897 325
Size: 1255 1129 1388 17 153 185 445 1498
Fragments arranged by size:
1498 1388 1255 1129 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2826 3120 4316
Size: 274 80 33 51 1443 849 294 1196
Cuts at: 4316 6028 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1196 849 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6030 6062 6030
Size: 32 6038
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2422 2422
Size: 6070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 2195 3264 3395 1870
Size: 325 1069 131 4545
Fragments arranged by size:
4545 1069 325 131
XmnI GAAnn'nnTTC
Cuts at: 2972 4199 2972
Size: 1227 4843
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3001 3878 276
Size: 53 83 186 936 1467 877 2468
Fragments arranged by size:
2468 1467 936 877 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SapI SfiI SwaI