Restriction map for
pFN21AA1145
(Circular) (Six-base) MAPSORT of: pFN21AA1145.seq Check: 6257 from: 1 to: 6043
pFN21AA1145 6043 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3853 278
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3386 1937
Size: 1449 4594
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 2411 2864 2993 4106 5490 5758 1632
Size: 512 267 453 129 1113 1384 268 1917
Fragments arranged by size:
1917 1384 1113 512 453 268 267 129
AfeI AGC'GCT
Cuts at: 2638 3026 2638
Size: 388 5655
AflII C'TTAA_G
Cuts at: 820 1017 2014 820
Size: 197 997 4849
Fragments arranged by size:
4849 997 197
AflIII A'CryG_T
Cuts at: 1203 4942 5919 1203
Size: 3739 977 1327
Fragments arranged by size:
3739 1327 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6043
AlwNI CAG_nnn'CTG
Cuts at: 2887 2983 3786 5358 2887
Size: 96 803 1572 3572
Fragments arranged by size:
3572 1572 803 96
ApaLI G'TGCA_C
Cuts at: 4100 5256 4100
Size: 1156 4887
ApoI r'AATT_y
Cuts at: 1493 2133 2584 3352 3578 3730 5839 1493
Size: 640 451 768 226 152 2109 1697
Fragments arranged by size:
2109 1697 768 640 451 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 3368 1870
Size: 73 1425 4545
Fragments arranged by size:
4545 1425 73
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3254 3364 4819 618
Size: 325 217 627 1467 110 1455 1842
Fragments arranged by size:
1842 1467 1455 627 325 217 110
BanII G_rGCy'C
Cuts at: 1391 2366 3087 3362 1391
Size: 975 721 275 4072
Fragments arranged by size:
4072 975 721 275
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5072
BbvCI CC'TCA_GC
Cuts at: 2826 2826
Size: 6043
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2303 2337 2552 2586 4234 4268 1903
Size: 34 366 34 215 34 1648 34 3678
Fragments arranged by size:
3678 1648 366 215 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2800 3935 5145 2800
Size: 1135 1210 3698
Fragments arranged by size:
3698 1210 1135
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3014 4654 136
Size: 107 122 71 631 605 1342 1640 1525
Fragments arranged by size:
1640 1525 1342 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6038 6038
Size: 6043
BlpI GC'TnA_GC
Cuts at: 2817 3460 2817
Size: 643 5400
Bme1580I G_kGCm'C
Cuts at: 1790 4104 5260 1790
Size: 2314 1156 2573
Fragments arranged by size:
2573 2314 1156
BmgBI CAC'GTC
Cuts at: 1318 2694 1318
Size: 1376 4667
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3307 3774 4732
Size: 416 53 83 399 24 1890 467 958
Cuts at: 4732 442
Size: 1753
Fragments arranged by size:
1890 1753 958 467 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2804 2939 3101 4703 1239
Size: 1565 135 162 1602 2579
Fragments arranged by size:
2579 1602 1565 162 135
Bpu10I CC'TnA_GC
Cuts at: 2826 2826
Size: 6043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4168 5033 5331 4168
Size: 865 298 4880
Fragments arranged by size:
4880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2488 4706 882
Size: 617 989 2218 2219
Fragments arranged by size:
2219 2218 989 617
BsaAI yAC'GTr
Cuts at: 493 1204 2425 3834 493
Size: 711 1221 1409 2702
Fragments arranged by size:
2702 1409 1221 711
BsaBI GATnn'nnATC
Cuts at: 2400 6037 2400
Size: 3637 2406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3850 4232 4867 275
Size: 53 83 186 936 2317 382 635 1451
Fragments arranged by size:
2317 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2541 4476 4874 5148 5295 5706 2541
Size: 1935 398 274 147 411 2878
Fragments arranged by size:
2878 1935 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5818
Fragments arranged by size:
5818 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2609 3011 1186
Size: 1423 402 4218
Fragments arranged by size:
4218 1423 402
BseYI C'CCAG_C
Cuts at: 2292 5246 5742 2292
Size: 2954 496 2593
Fragments arranged by size:
2954 2593 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2870 1163
Size: 573 1134 4336
Fragments arranged by size:
4336 1134 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2706 3493 4254 5282 1540
Size: 275 186 705 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 705 275 186
BsiHKAI G_wGCw'C
Cuts at: 2366 3087 4104 4189 5260 2366
Size: 721 1017 85 1071 3149
Fragments arranged by size:
3149 1071 1017 721 85
BsmI GAATG_Cn'
Cuts at: 3133 3554 3647 3133
Size: 421 93 5529
Fragments arranged by size:
5529 421 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3223 3223
Size: 6043
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2366 3087 3362 4104 4189 5260 1391
Size: 399 576 721 275 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 721 576 399 275 85
BspEI T'CCGG_A
Cuts at: 2541 5706 2541
Size: 3165 2878
BspHI T'CATG_A
Cuts at: 3174 3932 5662 3174
Size: 758 1730 3555
Fragments arranged by size:
3555 1730 758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2955 3398 844
Size: 2111 443 3489
Fragments arranged by size:
3489 2111 443
BsrBI CCG'CTC
Cuts at: 1394 1823 3930 1394
Size: 429 2107 3507
Fragments arranged by size:
3507 2107 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2446 2568 4538 4712 65
Size: 1183 1198 122 1970 174 1396
Fragments arranged by size:
1970 1396 1198 1183 174 122
BsrFI r'CCGG_y
Cuts at: 1700 1810 4687 4874 1700
Size: 110 2877 187 2869
Fragments arranged by size:
2877 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6043
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6043
BssSI C'ACGA_G
Cuts at: 4103 5115 4103
Size: 1012 5031
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3109 794
Size: 2315 3728
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6043
BstXI CCAn_nnnn'nTGG
Cuts at: 2729 2752 3251 2729
Size: 23 499 5521
Fragments arranged by size:
5521 499 23
BstYI r'GATC_y
Cuts at: 1972 3373 3740 4125 4142 5594 5680 5692 6038
Size: 1401 367 385 17 1452 86 12 346
Cuts at: 6038 1972
Size: 1977
Fragments arranged by size:
1977 1452 1401 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5491
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2157 2193 2272 2387 3190 487
Size: 683 56 931 36 79 115 803 3340
Fragments arranged by size:
3340 931 803 683 115 79 56 36
BtsI GCAGTG_nn'
Cuts at: 2109 2674 3559 4353 4373 2109
Size: 565 885 794 20 3779
Fragments arranged by size:
3779 885 794 565 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2703 2985 3490
Size: 54 1425 50 275 18 873 282 505
Cuts at: 3490 4379 6008 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 873 505 282 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2703 3490 1537
Size: 275 891 787 4090
Fragments arranged by size:
4090 891 787 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3227 3279 3973 1353
Size: 1874 52 694 3423
Fragments arranged by size:
3423 1874 694 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4630 5004 5150 387
Size: 1064 138 3041 374 146 1280
Fragments arranged by size:
3041 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2364 3085 2364
Size: 721 5322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2411 2804 2864 2939 2993 3101
Size: 393 512 267 393 60 75 54 108
Cuts at: 3101 4106 4703 5490 5758 1239
Size: 1005 597 787 268 1524
Fragments arranged by size:
1524 1005 787 597 512 393 393 268
267 108 75 60 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2891 2975 2891
Size: 84 5959
EcoO109I rG'GnC_Cy
Cuts at: 2622 2622
Size: 6043
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 6043
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 2562 2640 3028 5190 1678
Size: 884 78 388 2162 2531
Fragments arranged by size:
2531 2162 884 388 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 3220 3252 4684
Size: 32 135 32 584 32 1032 32 1432
Cuts at: 4684 4716 4758 4790 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1432 1032 584 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3633 669
Size: 988 281 1449 246 3079
Fragments arranged by size:
3079 1449 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5157 5341 1305
Size: 3852 184 2007
Fragments arranged by size:
3852 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2987 10
Size: 54 1425 343 1155 3066
Fragments arranged by size:
3066 1425 1155 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2955 3158 3188 4001 4360 4519 518
Size: 648 1789 203 30 813 359 159 2042
Fragments arranged by size:
2042 1789 813 648 359 203 159 30
MspA1I CmG'CkG
Cuts at: 1606 2296 2821 2929 3459 4136 5284 5529 1606
Size: 690 525 108 530 677 1148 245 2120
Fragments arranged by size:
2120 1148 690 677 530 525 245 108
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5491
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NruI TCG'CGA
Cuts at: 3209 3209
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 2476 3401 4946 1132
Size: 97 1247 925 1545 2229
Fragments arranged by size:
2229 1545 1247 925 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6043
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2452 1108
Size: 1344 4699
PfoI T'CCnGG_A
Cuts at: 2073 2085 2781 3039 2073
Size: 12 696 258 5077
Fragments arranged by size:
5077 696 258 12
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PmlI CAC'GTG
Cuts at: 2425 2425
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PstI C_TGCA'G
Cuts at: 830 2116 2301 3114 3395 830
Size: 1286 185 813 281 3478
Fragments arranged by size:
3478 1286 813 281 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2296 2821 2929 2296
Size: 525 108 5410
Fragments arranged by size:
5410 525 108
SacI G_AGCT'C
Cuts at: 2366 3087 2366
Size: 721 5322
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SfcI C'TryA_G
Cuts at: 826 1045 2112 2297 3110 3391 4526 5207 5398
Size: 219 1067 185 813 281 1135 681 191
Cuts at: 5398 826
Size: 1471
Fragments arranged by size:
1471 1135 1067 813 681 281 219 191
185
SfiI GGCCn_nnn'nGGCC
Cuts at: 3014 3014
Size: 6043
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 3370 1872
Size: 1498 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2014 4147 5048 5310 820
Size: 197 926 71 2133 901 262 1553
Fragments arranged by size:
2133 1553 926 901 262 197 71
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 2206 152
Size: 2054 3989
SphI G_CATG'C
Cuts at: 1132 2476 3401 1132
Size: 1344 925 3774
Fragments arranged by size:
3774 1344 925
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 3017 1517
Size: 1500 4543
StyI C'CwwG_G
Cuts at: 513 1065 3048 3482 513
Size: 552 1983 434 3074
Fragments arranged by size:
3074 1983 552 434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3238 4070 4087 4240 4425 4870 325
Size: 1255 1658 832 17 153 185 445 1498
Fragments arranged by size:
1658 1498 1255 832 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4289 6001 96
Size: 274 80 33 51 1443 2312 1712 138
Fragments arranged by size:
2312 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6003 6035 6003
Size: 32 6011
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 3368 1870
Size: 1498 4545
XmnI GAAnn'nnTTC
Cuts at: 4172 4172
Size: 6043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3851 276
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI
SrfI SwaI XcmI