Restriction map for
pFN21AA1139
(Circular) (Six-base) MAPSORT of: pFN21AA1139.seq Check: 6710 from: 1 to: 8311
pFN21AA1139 8311 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3188 4087 4771 3188
Size: 899 684 6728
Fragments arranged by size:
6728 899 684
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6121 278
Size: 53 83 186 936 4585 2468
Fragments arranged by size:
4585 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5654 1937
Size: 3717 4594
Acc65I G'GTAC_C
Cuts at: 5632 5632
Size: 8311
AclI AA'CG_TT
Cuts at: 1563 6438 6811 1563
Size: 4875 373 3063
Fragments arranged by size:
4875 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2876 6374 7758 8026 1632
Size: 1244 3498 1384 268 1917
Fragments arranged by size:
3498 1917 1384 1244 268
AfeI AGC'GCT
Cuts at: 2868 2868
Size: 8311
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8114
AflIII A'CryG_T
Cuts at: 1203 7210 8187 1203
Size: 6007 977 1327
Fragments arranged by size:
6007 1327 977
AgeI A'CCGG_T
Cuts at: 2054 7142 2054
Size: 5088 3223
AhdI GACnn_n'nnGTC
Cuts at: 7040 7040
Size: 8311
AleI CACnn'nnGTG
Cuts at: 1166 2520 1166
Size: 1354 6957
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2267 2385 2607 3141 1184
Size: 1083 118 222 534 6354
Fragments arranged by size:
6354 1083 534 222 118
AlwNI CAG_nnn'CTG
Cuts at: 2023 2068 2629 2833 3322 3349 3460 4811 4970
Size: 45 561 204 489 27 111 1351 159
Cuts at: 4970 5446 6054 7626 2023
Size: 476 608 1572 2708
Fragments arranged by size:
2708 1572 1351 608 561 489 476 204
159 111 45 27
ApaI G_GGCC'C
Cuts at: 4356 4594 4689 4710 4779 5426 4356
Size: 238 95 21 69 647 7241
Fragments arranged by size:
7241 647 238 95 69 21
ApaLI G'TGCA_C
Cuts at: 3708 6368 7524 3708
Size: 2660 1156 4495
Fragments arranged by size:
4495 2660 1156
ApoI r'AATT_y
Cuts at: 1493 2474 5620 5846 5998 8107 1493
Size: 981 3146 226 152 2109 1697
Fragments arranged by size:
3146 2109 1697 981 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6865 160
Size: 872 5833 1606
Fragments arranged by size:
5833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8311
AvaI C'yCGr_G
Cuts at: 1870 1943 3019 3168 3821 4554 4830 4917 5120
Size: 73 1076 149 653 733 276 87 203
Cuts at: 5120 5636 1870
Size: 516 4545
Fragments arranged by size:
4545 1076 733 653 516 276 203 149
87 73
AvrII C'CTAG_G
Cuts at: 4471 4471
Size: 8311
BamHI G'GATC_C
Cuts at: 2850 5641 6008 2850
Size: 2791 367 5153
Fragments arranged by size:
5153 2791 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3912 5287 5504 5522 5632
Size: 325 217 627 2125 1375 217 18 110
Cuts at: 5632 7087 618
Size: 1455 1842
Fragments arranged by size:
2125 1842 1455 1375 627 325 217 217
110 18
BanII G_rGCy'C
Cuts at: 1391 2111 2196 3269 4289 4356 4594 4682 4689
Size: 720 85 1073 1020 67 238 88 7
Cuts at: 4689 4710 4752 4779 5127 5426 5630 1391
Size: 21 42 27 348 299 204 4072
Fragments arranged by size:
4072 1073 1020 720 348 299 238 204
88 85 67 42 27 21 7
BbeI G_GCGC'C
Cuts at: 3916 3916
Size: 8311
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2456 928
Size: 971 557 6783
Fragments arranged by size:
6783 971 557
BbvCI CC'TCA_GC
Cuts at: 3179 3899 4437 4631 3179
Size: 720 538 194 6859
Fragments arranged by size:
6859 720 538 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2882 2916 5554 5588 6502 6536 1903
Size: 34 945 34 2638 34 914 34 3678
Fragments arranged by size:
3678 2638 945 914 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6203 7413 6203
Size: 1210 7101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2299 2321 2991
Size: 107 122 71 631 605 627 22 670
Cuts at: 2991 4416 4589 4714 6922 136
Size: 1425 173 125 2208 1525
Fragments arranged by size:
2208 1525 1425 670 631 627 605 173
125 122 107 71 22
BglII A'GATC_T
Cuts at: 3426 8306 3426
Size: 4880 3431
BlpI GC'TnA_GC
Cuts at: 3497 3645 3650 5224 5728 3497
Size: 148 5 1574 504 6080
Fragments arranged by size:
6080 1574 504 148 5
Bme1580I G_kGCm'C
Cuts at: 1790 2996 3278 3618 3712 4114 4211 4356 4516
Size: 1206 282 340 94 402 97 145 160
Cuts at: 4516 4594 4689 4710 4779 4935 5292 5426 5525
Size: 78 95 21 69 156 357 134 99
Cuts at: 5525 6372 7528 1790
Size: 847 1156 2573
Fragments arranged by size:
2573 1206 1156 847 402 357 340 282
160 156 145 134 99 97 95 94
78 69 21
BmgBI CAC'GTC
Cuts at: 1318 2743 3442 1318
Size: 1425 699 6187
Fragments arranged by size:
6187 1425 699
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3722 5378 5421
Size: 416 53 83 399 24 2305 1656 43
Cuts at: 5421 6042 7000 442
Size: 621 958 1753
Fragments arranged by size:
2305 1753 1656 958 621 416 399 83
53 43 24
BmtI G_CTAG'C
Cuts at: 1056 5363 1056
Size: 4307 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2309 2393 2555 2747 3354 3482 3533
Size: 971 99 84 162 192 607 128 51
Cuts at: 3533 3716 3977 5082 5172 5468 6971 1239
Size: 183 261 1105 90 296 1503 2579
Fragments arranged by size:
2579 1503 1105 971 607 296 261 192
183 162 128 99 90 84 51
Bpu10I CC'TnA_GC
Cuts at: 2217 3179 3386 3899 4224 4386 4406 4437 4631
Size: 962 207 513 325 162 20 31 194
Cuts at: 4631 5355 2217
Size: 724 5173
Fragments arranged by size:
5173 962 724 513 325 207 194 162
31 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 2855 5267 6436 7301 7599 2471
Size: 384 2412 1169 865 298 3183
Fragments arranged by size:
3183 2412 1169 865 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6974 882
Size: 617 5475 2219
Fragments arranged by size:
5475 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2760 6102 493
Size: 711 1556 3342 2702
Fragments arranged by size:
3342 2702 1556 711
BsaBI GATnn'nnATC
Cuts at: 2645 3944 8305 2645
Size: 1299 4361 2651
Fragments arranged by size:
4361 2651 1299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2453 3913 4820 5577
Size: 53 83 186 936 920 1460 907 757
Cuts at: 5577 5598 6118 6500 7135 275
Size: 21 520 382 635 1451
Fragments arranged by size:
1460 1451 936 920 907 757 635 520
382 186 83 53 21
BsaWI w'CCGG_w
Cuts at: 2054 2751 3931 4521 6744 7142 7416 7563 7974
Size: 697 1180 590 2223 398 274 147 411
Cuts at: 7974 2054
Size: 2391
Fragments arranged by size:
2391 2223 1180 697 590 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3689 3719 4056 4086 1178
Size: 30 165 30 2286 30 337 30 5403
Fragments arranged by size:
5403 2286 337 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 3806 3986 4554 4808 4811 4814 5085
Size: 1210 1410 180 568 254 3 3 271
Cuts at: 5085 5270 5382 1186
Size: 185 112 4115
Fragments arranged by size:
4115 1410 1210 568 271 254 185 180
112 3 3
BseYI C'CCAG_C
Cuts at: 2496 2636 3193 3315 3671 3844 3973 4091 4160
Size: 140 557 122 356 173 129 118 69
Cuts at: 4160 4175 4549 4649 4772 5115 5126 5168 5334
Size: 15 374 100 123 343 11 42 166
Cuts at: 5334 5436 5528 7514 8010 2496
Size: 102 92 1986 496 2797
Fragments arranged by size:
2797 1986 557 496 374 356 343 173
166 140 129 123 122 118 102 100
92 69 42 15 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2246 2345 2355 2567 5314 5460
Size: 573 345 165 99 10 212 2747 146
Cuts at: 5460 1163
Size: 4014
Fragments arranged by size:
4014 2747 573 345 212 165 146 99
10
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2959 5265 5761 6522 7550 1540
Size: 275 186 958 2306 496 761 1028 2301
Fragments arranged by size:
2306 2301 1028 958 761 496 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 3269 3712 5411 6372 6457 7528 2196
Size: 1073 443 1699 961 85 1071 2979
Fragments arranged by size:
2979 1699 1073 1071 961 443 85
BsmI GAATG_Cn'
Cuts at: 5822 5915 5822
Size: 93 8218
BsmBI CGTCTCn'nnnn_
Cuts at: 4645 4645
Size: 8311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2196 2996 3269 3278 3618 3712
Size: 399 321 85 800 273 9 340 94
Cuts at: 3712 4114 4211 4289 4356 4516 4594 4682 4689
Size: 402 97 78 67 160 78 88 7
Cuts at: 4689 4710 4752 4779 4935 5127 5292 5411 5426
Size: 21 42 27 156 192 165 119 15
Cuts at: 5426 5525 5630 6372 6457 7528 1391
Size: 99 105 742 85 1071 2174
Fragments arranged by size:
2174 1071 800 742 402 399 340 321
273 192 165 160 156 119 105 99
97 94 88 85 85 78 78 67
42 27 21 15 9 7
BspEI T'CCGG_A
Cuts at: 2751 3931 4521 7974 2751
Size: 1180 590 3453 3088
Fragments arranged by size:
3453 3088 1180 590
BspHI T'CATG_A
Cuts at: 6200 7930 6200
Size: 1730 6581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3188 3699 4087 4741 4771 5666 844
Size: 2344 511 388 654 30 895 3489
Fragments arranged by size:
3489 2344 895 654 511 388 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3414 4086 4216 6198 1394
Size: 429 1591 672 130 1982 3507
Fragments arranged by size:
3507 1982 1591 672 429 130
BsrDI GCAATG_nn'
Cuts at: 65 1248 2251 6806 6980 65
Size: 1183 1003 4555 174 1396
Fragments arranged by size:
4555 1396 1183 1003 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2054 2283 3251 3542 3915 4392 6955
Size: 110 244 229 968 291 373 477 2563
Cuts at: 6955 7142 1700
Size: 187 2869
Fragments arranged by size:
2869 2563 968 477 373 291 244 229
187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8311
BssHII G'CGCG_C
Cuts at: 1925 2311 1925
Size: 386 7925
BssSI C'ACGA_G
Cuts at: 2681 2978 6371 7383 2681
Size: 297 3393 1012 3609
Fragments arranged by size:
3609 3393 1012 297
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2833 794
Size: 2039 6272
BstBI TT'CG_AA
Cuts at: 7115 7115
Size: 8311
BstEII G'GTnAC_C
Cuts at: 1184 3601 1184
Size: 2417 5894
BstXI CCAn_nnnn'nTGG
Cuts at: 3293 3769 3293
Size: 476 7835
BstYI r'GATC_y
Cuts at: 1972 2352 2850 3426 4171 5641 6008 6393 6410
Size: 380 498 576 745 1470 367 385 17
Cuts at: 6410 7862 7948 7960 8306 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1470 1452 745 576 498 385 380
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2845 3579 4812 2845
Size: 734 1233 6344
Fragments arranged by size:
6344 1233 734
BtgI C'CryG_G
Cuts at: 513 1065 2004 3286 3762 3989 4041 5396 513
Size: 552 939 1282 476 227 52 1355 3428
Fragments arranged by size:
3428 1355 1282 939 552 476 227 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3640 487
Size: 683 56 2414 5158
Fragments arranged by size:
5158 2414 683 56
BtsI GCAGTG_nn'
Cuts at: 2528 2898 3226 3253 3320 3522 4516 5006 5018
Size: 370 328 27 67 202 994 490 12
Cuts at: 5018 5066 5827 6621 6641 2528
Size: 48 761 794 20 4198
Fragments arranged by size:
4198 994 794 761 490 370 328 202
67 48 27 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8276
DraI TTT'AAA
Cuts at: 5615 5615
Size: 8311
DraIII CAC_nnn'GTG
Cuts at: 4727 4727
Size: 8311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5461 7318 809
Size: 507 4145 1857 1802
Fragments arranged by size:
4145 1857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2479 2602 2956
Size: 54 1425 50 275 18 649 123 354
Cuts at: 2956 3283 3402 3446 3591 3774 3921 3942 4038
Size: 327 119 44 145 183 147 21 96
Cuts at: 4038 4129 4246 4347 4499 5037 5262 5758 6647
Size: 91 117 101 152 538 225 496 889
Cuts at: 6647 8276 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 649 538 496 354 327
275 225 183 152 147 145 123 119
117 101 96 91 54 50 44 43
21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2956 5262 5758 1537
Size: 275 1144 2306 496 4090
Fragments arranged by size:
4090 2306 1144 496 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5489 6241 1353
Size: 4136 752 3423
Fragments arranged by size:
4136 3423 752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3878 6898 7272 7418 387
Size: 1064 138 2289 3020 374 146 1280
Fragments arranged by size:
3020 2289 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2194 3267 2194
Size: 1073 7238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2309 2393 2555 2747 2876 3354
Size: 393 578 99 84 162 192 129 478
Cuts at: 3354 3482 3533 3716 3977 5082 5172 5468 6374
Size: 128 51 183 261 1105 90 296 906
Cuts at: 6374 6971 7758 8026 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1105 906 787 597 578 478 393
296 268 261 192 183 162 129 128
99 90 84 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3884 4202 4248 5282 3884
Size: 318 46 1034 6913
Fragments arranged by size:
6913 1034 318 46
EcoO109I rG'GnC_Cy
Cuts at: 2020 3583 3877 3895 4253 4353 4604 4685 4706
Size: 1563 294 18 358 100 251 81 21
Cuts at: 4706 4707 4808 2020
Size: 1 101 5523
Fragments arranged by size:
5523 1563 358 294 251 101 100 81
21 18 1
EcoRI G'AATT_C
Cuts at: 5620 5998 5620
Size: 378 7933
FspI TGC'GCA
Cuts at: 6817 6817
Size: 8311
HaeII r_GCGC'y
Cuts at: 1678 2678 2777 2870 3916 4393 4630 4894 5366
Size: 1000 99 93 1046 477 237 264 472
Cuts at: 5366 5535 7458 1678
Size: 169 1923 2531
Fragments arranged by size:
2531 1923 1046 1000 477 472 264 237
169 99 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2601 2625 2633
Size: 32 135 32 566 32 431 24 8
Cuts at: 2633 2657 6952 6984 7026 7058 1373
Size: 24 4295 32 42 32 2626
Fragments arranged by size:
4295 2626 566 431 135 42 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2230 2437 3814 5655 5901 669
Size: 988 281 292 207 1377 1841 246 3079
Fragments arranged by size:
3079 1841 1377 988 292 281 246 207
HindIII A'AGCT_T
Cuts at: 748 4226 748
Size: 3478 4833
HpaI GTT'AAC
Cuts at: 5901 5901
Size: 8311
KasI G'GCGC_C
Cuts at: 3912 3912
Size: 8311
KpnI G_GTAC'C
Cuts at: 5636 5636
Size: 8311
MfeI C'AATT_G
Cuts at: 5910 5910
Size: 8311
MluI A'CGCG_T
Cuts at: 8187 8187
Size: 8311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2445 5177 7425 7609 1305
Size: 1140 2732 2248 184 2007
Fragments arranged by size:
2732 2248 2007 1140 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2604 3285 3404 3923 3944
Size: 54 1425 343 772 681 119 519 21
Cuts at: 3944 4131 4248 5039 10
Size: 187 117 791 3282
Fragments arranged by size:
3282 1425 791 772 681 519 343 187
119 117 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2520 2933 3599 6269 6628 6787 518
Size: 648 1354 413 666 2670 359 159 2042
Fragments arranged by size:
2670 2042 1354 666 648 413 359 159
MspA1I CmG'CkG
Cuts at: 1606 2260 2500 2961 3258 3457 3511 3739 3750
Size: 654 240 461 297 199 54 228 11
Cuts at: 3750 3983 4164 4904 5060 5338 5474 5593 5727
Size: 233 181 740 156 278 136 119 134
Cuts at: 5727 6404 7552 7797 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 740 677 654 461 297 278
245 240 233 228 199 181 156 136
134 119 54 11
NaeI GCC'GGC
Cuts at: 2285 3253 3544 3917 2285
Size: 968 291 373 6679
Fragments arranged by size:
6679 968 373 291
NarI GG'CG_CC
Cuts at: 3913 3913
Size: 8311
NcoI C'CATG_G
Cuts at: 513 1065 2004 3286 3762 4041 513
Size: 552 939 1282 476 279 4783
Fragments arranged by size:
4783 1282 939 552 476 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8311
NgoMIV G'CCGG_C
Cuts at: 2283 3251 3542 3915 2283
Size: 968 291 373 6679
Fragments arranged by size:
6679 968 373 291
NheI G'CTAG_C
Cuts at: 1052 5359 1052
Size: 4307 4004
NotI GC'GGCC_GC
Cuts at: 5758 5758
Size: 8311
NspI r_CATG'y
Cuts at: 1132 1229 2254 5669 7214 1132
Size: 97 1025 3415 1545 2229
Fragments arranged by size:
3415 2229 1545 1025 97
PasI CC'CwG_GG
Cuts at: 1348 4282 4307 4329 4682 5204 1348
Size: 2934 25 22 353 522 4455
Fragments arranged by size:
4455 2934 522 353 25 22
PciI A'CATG_T
Cuts at: 7210 7210
Size: 8311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4319 4673 5084 1108
Size: 3211 354 411 4335
Fragments arranged by size:
4335 3211 411 354
PfoI T'CCnGG_A
Cuts at: 3107 3107
Size: 8311
PmeI GTTT'AAAC
Cuts at: 5615 5615
Size: 8311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6380 6412 7893 7925 6380
Size: 32 1481 32 6766
Fragments arranged by size:
6766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 3583 4253 2020
Size: 1563 670 6078
Fragments arranged by size:
6078 1563 670
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8311
PsiI TTA'TAA
Cuts at: 5881 5881
Size: 8311
PspOMI G'GGCC_C
Cuts at: 4352 4590 4685 4706 4775 5422 4352
Size: 238 95 21 69 647 7241
Fragments arranged by size:
7241 647 238 95 69 21
PstI C_TGCA'G
Cuts at: 830 3064 3540 3801 3888 5013 5663 830
Size: 2234 476 261 87 1125 650 3478
Fragments arranged by size:
3478 2234 1125 650 476 261 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8311
PvuII CAG'CTG
Cuts at: 2500 3457 3511 3739 3983 5338 5474 5593 2500
Size: 957 54 228 244 1355 136 119 5218
Fragments arranged by size:
5218 1355 957 244 228 136 119 54
SacI G_AGCT'C
Cuts at: 2196 3269 2196
Size: 1073 7238
SalI G'TCGA_C
Cuts at: 1936 5653 1936
Size: 3717 4594
SapI GCTCTTCn'nnn_
Cuts at: 5489 5489
Size: 8311
SbfI CC_TGCA'GG
Cuts at: 5663 5663
Size: 8311
ScaI AGT'ACT
Cuts at: 6559 8271 6559
Size: 1712 6599
SexAI A'CCwGG_T
Cuts at: 3342 3342
Size: 8311
SfcI C'TryA_G
Cuts at: 826 1045 3060 3154 3536 3797 3884 3978 4031
Size: 219 2015 94 382 261 87 94 53
Cuts at: 4031 5009 5659 6794 7475 7666 826
Size: 978 650 1135 681 191 1471
Fragments arranged by size:
2015 1471 1135 978 681 650 382 261
219 191 94 94 87 53
SfoI GGC'GCC
Cuts at: 3914 3914
Size: 8311
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8311
SmaI CCC'GGG
Cuts at: 1872 3021 5638 1872
Size: 1149 2617 4545
Fragments arranged by size:
4545 2617 1149
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 2870 5282 6415 7316 7578
Size: 197 926 543 384 2412 1133 901 262
Cuts at: 7578 820
Size: 1553
Fragments arranged by size:
2412 1553 1133 926 901 543 384 262
197
SnaBI TAC'GTA
Cuts at: 493 2760 6102 493
Size: 2267 3342 2702
Fragments arranged by size:
3342 2702 2267
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8311
SphI G_CATG'C
Cuts at: 1132 2254 5669 1132
Size: 1122 3415 3774
Fragments arranged by size:
3774 3415 1122
SspI AAT'ATT
Cuts at: 5 52 6235 5
Size: 47 6183 2081
Fragments arranged by size:
6183 2081 47
StuI AGG'CCT
Cuts at: 1517 2843 1517
Size: 1326 6985
StyI C'CwwG_G
Cuts at: 513 1065 2004 3286 3762 3924 4041 4249 4471
Size: 552 939 1282 476 162 117 208 222
Cuts at: 4471 4689 5143 5508 5750 513
Size: 218 454 365 242 3074
Fragments arranged by size:
3074 1282 939 552 476 454 365 242
222 218 208 162 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2720 3097 3449 5348 6338 6355 6508
Size: 1255 1140 377 352 1899 990 17 153
Cuts at: 6508 6693 7138 325
Size: 185 445 1498
Fragments arranged by size:
1899 1498 1255 1140 990 445 377 352
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6557 8269 96
Size: 274 80 33 51 1443 4580 1712 138
Fragments arranged by size:
4580 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3353 3385 3689 3721 4056 4088 8271 8303 3353
Size: 32 304 32 335 32 4183 32 3361
Fragments arranged by size:
4183 3361 335 304 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8311
XbaI T'CTAG_A
Cuts at: 5647 5647
Size: 8311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2144 2428 3769 3952 5464 5548 2144
Size: 284 1341 183 1512 84 4907
Fragments arranged by size:
4907 1512 1341 284 183 84
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8311
XmaI C'CCGG_G
Cuts at: 1870 3019 5636 1870
Size: 1149 2617 4545
Fragments arranged by size:
4545 2617 1149
XmnI GAAnn'nnTTC
Cuts at: 2792 6440 2792
Size: 3648 4663
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6119 276
Size: 53 83 186 936 4585 2468
Fragments arranged by size:
4585 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BplI BsiWI BstZ17I EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SfiI SrfI SwaI