Restriction map for
pFN21AA1132
(Circular) (Six-base) MAPSORT of: pFN21AA1132.seq Check: 9751 from: 1 to: 10864
pFN21AA1132 10864 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2095 7285 2095
Size: 5190 5674
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8674 278
Size: 53 83 186 936 7138 2468
Fragments arranged by size:
7138 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5154 8207 1937
Size: 3217 3053 4594
Fragments arranged by size:
4594 3217 3053
Acc65I G'GTAC_C
Cuts at: 2181 8185 2181
Size: 6004 4860
AclI AA'CG_TT
Cuts at: 1563 8991 9364 1563
Size: 7428 373 3063
Fragments arranged by size:
7428 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 2439 2996 3229 4580 4582 6430 6887
Size: 447 360 557 233 1351 2 1848 457
Cuts at: 6887 8927 10311 10579 1632
Size: 2040 1384 268 1917
Fragments arranged by size:
2040 1917 1848 1384 1351 557 457 447
360 268 233 2
AfeI AGC'GCT
Cuts at: 6317 8005 6317
Size: 1688 9176
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10667
AflIII A'CryG_T
Cuts at: 1203 6087 7158 9763 10740 1203
Size: 4884 1071 2605 977 1327
Fragments arranged by size:
4884 2605 1327 1071 977
AgeI A'CCGG_T
Cuts at: 3453 7230 9695 3453
Size: 3777 2465 4622
Fragments arranged by size:
4622 3777 2465
AhdI GACnn_n'nnGTC
Cuts at: 9593 9593
Size: 10864
AleI CACnn'nnGTG
Cuts at: 1166 3286 6938 8159 1166
Size: 2120 3652 1221 3871
Fragments arranged by size:
3871 3652 2120 1221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4733 5561 6065 1184
Size: 3549 828 504 5983
Fragments arranged by size:
5983 3549 828 504
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4577 4609 4577
Size: 32 10832
AlwNI CAG_nnn'CTG
Cuts at: 2666 2817 4243 5506 5788 5960 7064 7595 8607
Size: 151 1426 1263 282 172 1104 531 1012
Cuts at: 8607 10179 2666
Size: 1572 3351
Fragments arranged by size:
3351 1572 1426 1263 1104 1012 531 282
172 151
ApaI G_GGCC'C
Cuts at: 3331 4971 5232 5302 6962 7885 8051 3331
Size: 1640 261 70 1660 923 166 6144
Fragments arranged by size:
6144 1660 1640 923 261 166 70
ApaLI G'TGCA_C
Cuts at: 3457 6091 8921 10077 3457
Size: 2634 2830 1156 4244
Fragments arranged by size:
4244 2830 2634 1156
ApoI r'AATT_y
Cuts at: 1493 3812 3950 8173 8399 8551 10660 1493
Size: 2319 138 4223 226 152 2109 1697
Fragments arranged by size:
4223 2319 2109 1697 226 152 138
AseI AT'TA_AT
Cuts at: 160 1032 9418 160
Size: 872 8386 1606
Fragments arranged by size:
8386 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10864
AvaI C'yCGr_G
Cuts at: 1870 1943 2752 3266 3865 3925 5696 7440 8087
Size: 73 809 514 599 60 1771 1744 647
Cuts at: 8087 8189 1870
Size: 102 4545
Fragments arranged by size:
4545 1771 1744 809 647 599 514 102
73 60
BamHI G'GATC_C
Cuts at: 8194 8561 8194
Size: 367 10497
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2137 2181 2873 3302
Size: 325 217 627 282 68 44 692 429
Cuts at: 3302 3395 4325 5029 5894 6018 7544 8185 9640
Size: 93 930 704 865 124 1526 641 1455
Cuts at: 9640 618
Size: 1842
Fragments arranged by size:
1842 1526 1455 930 865 704 692 641
627 429 325 282 217 124 93 68
44
BanII G_rGCy'C
Cuts at: 1391 2159 2348 3026 3102 3331 3345 3962 4665
Size: 768 189 678 76 229 14 617 703
Cuts at: 4665 4935 4971 5232 5302 5346 6392 6441 6962
Size: 270 36 261 70 44 1046 49 521
Cuts at: 6962 7316 7761 7885 8051 8072 8183 1391
Size: 354 445 124 166 21 111 4072
Fragments arranged by size:
4072 1046 768 703 678 617 521 445
354 270 261 229 189 166 124 111
76 70 49 44 36 21 14
BbeI G_GCGC'C
Cuts at: 3306 3306
Size: 10864
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2425 2984 5994 928
Size: 971 526 559 3010 5798
Fragments arranged by size:
5798 3010 971 559 526
BbvCI CC'TCA_GC
Cuts at: 6069 6187 6069
Size: 118 10746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3571 3605 5231 5265 9055 9089 1903
Size: 34 1634 34 1626 34 3790 34 3678
Fragments arranged by size:
3790 3678 1634 1626 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8756 9966 8756
Size: 1210 9654
BclI T'GATC_A
Cuts at: 1548 3912 4374 1548
Size: 2364 462 8038
Fragments arranged by size:
8038 2364 462
BfrBI ATG'CAT
Cuts at: 4011 4011
Size: 10864
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8015 9475 136
Size: 107 122 71 631 605 6343 1460 1525
Fragments arranged by size:
6343 1525 1460 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3969 10859 3969
Size: 6890 3974
BlpI GC'TnA_GC
Cuts at: 3101 7983 8281 3101
Size: 4882 298 5684
Fragments arranged by size:
5684 4882 298
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2647 3016 3331 3461 3792 4071 4221
Size: 352 505 369 315 130 331 279 150
Cuts at: 4221 4971 5232 5274 5302 5897 6021 6095 6962
Size: 750 261 42 28 595 124 74 867
Cuts at: 6962 7668 7885 8051 8925 10081 1790
Size: 706 217 166 874 1156 2573
Fragments arranged by size:
2573 1156 874 867 750 706 595 505
369 352 331 315 279 261 217 166
150 130 124 74 42 28
BmgBI CAC'GTC
Cuts at: 1318 2212 2230 3694 4273 5320 7754 1318
Size: 894 18 1464 579 1047 2434 4428
Fragments arranged by size:
4428 2434 1464 1047 894 579 18
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5179 6691 6824
Size: 416 53 83 399 24 3762 1512 133
Cuts at: 6824 7037 8595 9553 442
Size: 213 1558 958 1753
Fragments arranged by size:
3762 1753 1558 1512 958 416 399 213
133 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5619 1056
Size: 4563 6301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3295 4567 7432 3103
Size: 192 1272 2865 6535
Fragments arranged by size:
6535 2865 1272 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3913 4703 5282 6292 6569 7484 8056 9524
Size: 2674 790 579 1010 277 915 572 1468
Cuts at: 9524 1239
Size: 2579
Fragments arranged by size:
2674 2579 1468 1010 915 790 579 572
277
Bpu10I CC'TnA_GC
Cuts at: 6069 6187 6069
Size: 118 10746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2768 3233 3665 3971 4550 5156 6323 6735 7222
Size: 465 432 306 579 606 1167 412 487
Cuts at: 7222 8989 9854 10152 2768
Size: 1767 865 298 3480
Fragments arranged by size:
3480 1767 1167 865 606 579 487 465
432 412 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2618 8121 9527 882
Size: 617 1119 5503 1406 2219
Fragments arranged by size:
5503 2219 1406 1119 617
BsaAI yAC'GTr
Cuts at: 493 1204 3775 6090 6351 6639 8655 493
Size: 711 2571 2315 261 288 2016 2702
Fragments arranged by size:
2702 2571 2315 2016 711 288 261
BsaBI GATnn'nnATC
Cuts at: 10858 10858
Size: 10864
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2577 3303 5367 7107
Size: 53 83 186 936 1044 726 2064 1740
Cuts at: 7107 8671 9053 9688 275
Size: 1564 382 635 1451
Fragments arranged by size:
2064 1740 1564 1451 1044 936 726 635
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2340 3453 5754 7230 7794 9297 9695 9969 10116
Size: 1113 2301 1476 564 1503 398 274 147
Cuts at: 10116 10527 2340
Size: 411 2677
Fragments arranged by size:
2677 2301 1503 1476 1113 564 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2249 2279 2486 2516 2567
Size: 30 165 30 846 30 207 30 51
Cuts at: 2567 2597 3710 3740 6464 6494 8017 8047 1178
Size: 30 1113 30 2724 30 1523 30 3995
Fragments arranged by size:
3995 2724 1523 1113 846 207 165 51
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4964 6296 7445 7481 1186
Size: 3778 1332 1149 36 4569
Fragments arranged by size:
4569 3778 1332 1149 36
BseYI C'CCAG_C
Cuts at: 2162 3521 3747 4200 4467 4728 5273 5522 5717
Size: 1359 226 453 267 261 545 249 195
Cuts at: 5717 5869 7317 7942 7960 8000 8021 10067 10563
Size: 152 1448 625 18 40 21 2046 496
Cuts at: 10563 2162
Size: 2463
Fragments arranged by size:
2463 2046 1448 1359 625 545 496 453
267 261 249 226 195 152 40 21
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2296 2480 2728 3716 3748 4417 6078
Size: 573 560 184 248 988 32 669 1661
Cuts at: 6078 1163
Size: 5949
Fragments arranged by size:
5949 1661 988 669 573 560 248 184
32
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2151 5821 8314 9075 10103 1540
Size: 275 186 150 3670 2493 761 1028 2301
Fragments arranged by size:
3670 2493 2301 1028 761 275 186 150
BsiHKAI G_wGCw'C
Cuts at: 3461 3962 4131 4935 5550 6095 7142 7583 8099
Size: 501 169 804 615 545 1047 441 516
Cuts at: 8099 8925 9010 10081 3461
Size: 826 85 1071 4244
Fragments arranged by size:
4244 1071 1047 826 804 615 545 516
501 441 169 85
BsiWI C'GTAC_G
Cuts at: 5533 6838 5533
Size: 1305 9559
BsmI GAATG_Cn'
Cuts at: 8375 8468 8375
Size: 93 10771
BsmBI CGTCTCn'nnnn_
Cuts at: 3104 3241 3985 4555 5723 7114 3104
Size: 137 744 570 1168 1391 6854
Fragments arranged by size:
6854 1391 1168 744 570 137
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2159 2348 2647 3016 3026 3102
Size: 399 352 17 189 299 369 10 76
Cuts at: 3102 3331 3345 3461 3792 3962 4071 4131 4221
Size: 229 14 116 331 170 109 60 90
Cuts at: 4221 4665 4935 4971 5232 5274 5302 5346 5550
Size: 444 270 36 261 42 28 44 204
Cuts at: 5550 5897 6021 6095 6392 6441 6962 7142 7316
Size: 347 124 74 297 49 521 180 174
Cuts at: 7316 7583 7668 7761 7885 8051 8072 8099 8183
Size: 267 85 93 124 166 21 27 84
Cuts at: 8183 8925 9010 10081 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 521 444 399 369 352
347 331 299 297 270 267 261 229
204 189 180 174 170 166 124 124
116 109 93 90 85 85 84 76
74 60 49 44 42 36 28 27
21 17 14 10
BspEI T'CCGG_A
Cuts at: 2340 7794 10527 2340
Size: 5454 2733 2677
Fragments arranged by size:
5454 2733 2677
BspHI T'CATG_A
Cuts at: 3954 8753 10483 3954
Size: 4799 1730 4335
Fragments arranged by size:
4799 4335 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2095 4725 6510 7285 7713 8219 844
Size: 1251 2630 1785 775 428 506 3489
Fragments arranged by size:
3489 2630 1785 1251 775 506 428
BsrBI CCG'CTC
Cuts at: 1394 1823 4191 4450 4958 5455 8751 1394
Size: 429 2368 259 508 497 3296 3507
Fragments arranged by size:
3507 3296 2368 508 497 429 259
BsrDI GCAATG_nn'
Cuts at: 65 1248 2644 3204 6691 9359 9533 65
Size: 1183 1396 560 3487 2668 174 1396
Fragments arranged by size:
3487 2668 1396 1396 1183 560 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2330 2720 2793 2981 3453 5710 5816
Size: 110 520 390 73 188 472 2257 106
Cuts at: 5816 6021 6555 7230 7946 9508 9695 1700
Size: 205 534 675 716 1562 187 2869
Fragments arranged by size:
2869 2257 1562 716 675 534 520 472
390 205 188 187 110 106 73
BsrGI T'GTAC_A
Cuts at: 96 2550 4875 96
Size: 2454 2325 6085
Fragments arranged by size:
6085 2454 2325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10864
BssSI C'ACGA_G
Cuts at: 8037 8924 9936 8037
Size: 887 1012 8965
Fragments arranged by size:
8965 1012 887
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5788 794
Size: 4994 5870
BstBI TT'CG_AA
Cuts at: 3810 7738 9668 3810
Size: 3928 1930 5006
Fragments arranged by size:
5006 3928 1930
BstEII G'GTnAC_C
Cuts at: 1184 2107 2890 3319 5017 5842 1184
Size: 923 783 429 1698 825 6206
Fragments arranged by size:
6206 1698 923 825 783 429
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2125 2689 4852 5260 6038 6547 2011
Size: 114 564 2163 408 778 509 6328
Fragments arranged by size:
6328 2163 778 564 509 408 114
BstYI r'GATC_y
Cuts at: 1972 2337 3432 3969 4239 4362 4686 5950 8194
Size: 365 1095 537 270 123 324 1264 2244
Cuts at: 8194 8561 8946 8963 10415 10501 10513 10859 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2244 1977 1452 1264 1095 537 385 367
365 346 324 270 123 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2124 2151 2316 2539 2730 3094 3315
Size: 552 1059 27 165 223 191 364 221
Cuts at: 3315 4579 4974 5136 5773 6009 6427 6962 7308
Size: 1264 395 162 637 236 418 535 346
Cuts at: 7308 7767 513
Size: 459 3610
Fragments arranged by size:
3610 1264 1059 637 552 535 459 418
395 364 346 236 223 221 191 165
162 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3997 4384 4504 487
Size: 683 56 2771 387 120 6847
Fragments arranged by size:
6847 2771 683 387 120 56
BtsI GCAGTG_nn'
Cuts at: 3487 3736 4921 5548 5626 6044 6066 6824 8380
Size: 249 1185 627 78 418 22 758 1556
Cuts at: 8380 9174 9194 3487
Size: 794 20 5157
Fragments arranged by size:
5157 1556 1185 794 758 627 418 249
78 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10864
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6091 6126 529
Size: 35 5527 35 5267
Fragments arranged by size:
5527 5267 35 35
DraI TTT'AAA
Cuts at: 8168 8168
Size: 10864
DraIII CAC_nnn'GTG
Cuts at: 2731 4216 4445 7503 7781 2731
Size: 1485 229 3058 278 5814
Fragments arranged by size:
5814 3058 1485 278 229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4547 5150 5324 9871 809
Size: 507 3231 603 174 4547 1802
Fragments arranged by size:
4547 3231 1802 603 507 174
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2187 2427
Size: 54 1425 50 275 18 318 39 240
Cuts at: 2427 2722 2791 3294 3832 4228 4368 4909 5322
Size: 295 69 503 538 396 140 541 413
Cuts at: 5322 5704 5799 5818 5994 6096 6807 7172 7926
Size: 382 95 19 176 102 711 365 754
Cuts at: 7926 8311 9200 10829 8
Size: 385 889 1629 43
Fragments arranged by size:
1629 1425 889 754 711 541 538 503
413 396 385 382 365 318 295 275
240 176 140 102 95 69 54 50
43 39 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2148 5818 8311 1537
Size: 275 336 3670 2493 4090
Fragments arranged by size:
4090 3670 2493 336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2458 3131 4275 5239 5753 6470 6836 6842
Size: 1105 673 1144 964 514 717 366 6
Cuts at: 6842 7423 8794 1353
Size: 581 1371 3423
Fragments arranged by size:
3423 1371 1144 1105 964 717 673 581
514 366 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2263 4256 9451 9825 9971 387
Size: 1064 138 674 1993 5195 374 146 1280
Fragments arranged by size:
5195 1993 1280 1064 674 374 146 138
EcoICRI GAG'CTC
Cuts at: 3960 4933 3960
Size: 973 9891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2439 2996 3229 3913 4580 4582
Size: 393 447 360 557 233 684 667 2
Cuts at: 4582 4703 5282 6292 6430 6569 6887 7484 8056
Size: 121 579 1010 138 139 318 597 572
Cuts at: 8056 8927 9524 10311 10579 1239
Size: 871 597 787 268 1524
Fragments arranged by size:
1524 1010 871 787 684 667 597 597
579 572 557 447 393 360 318 268
233 139 138 121 2
EcoNI CCTnn'n_nnAGG
Cuts at: 4280 4304 6942 7367 4280
Size: 24 2638 425 7777
Fragments arranged by size:
7777 2638 425 24
EcoO109I rG'GnC_Cy
Cuts at: 3150 3328 3425 3675 4968 5228 5229 5299 5579
Size: 178 97 250 1293 260 1 70 280
Cuts at: 5579 5866 6567 6583 6958 7145 7881 7882 8047
Size: 287 701 16 375 187 736 1 165
Cuts at: 8047 8136 3150
Size: 89 5878
Fragments arranged by size:
5878 1293 736 701 375 287 280 260
250 187 178 165 97 89 70 16
1 1
EcoRI G'AATT_C
Cuts at: 3812 3950 8173 8551 3812
Size: 138 4223 378 6125
Fragments arranged by size:
6125 4223 378 138
FseI GG_CCGG'CC
Cuts at: 2724 2797 2724
Size: 73 10791
FspI TGC'GCA
Cuts at: 6653 9370 6653
Size: 2717 8147
HaeII r_GCGC'y
Cuts at: 1678 3306 4051 6319 8007 10011 1678
Size: 1628 745 2268 1688 2004 2531
Fragments arranged by size:
2531 2268 2004 1688 1628 745
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2393 2425 2486 2518 2567
Size: 32 135 32 821 32 61 32 49
Cuts at: 2567 2599 3897 3929 7137 7169 7508 7540 9505
Size: 32 1298 32 3208 32 339 32 1965
Cuts at: 9505 9537 9579 9611 1373
Size: 32 42 32 2626
Fragments arranged by size:
3208 2626 1965 1298 821 339 135 61
49 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3262 6133 7084 8208 8454 669
Size: 988 281 1324 2871 951 1124 246 3079
Fragments arranged by size:
3079 2871 1324 1124 988 951 281 246
HindIII A'AGCT_T
Cuts at: 748 6925 748
Size: 6177 4687
HpaI GTT'AAC
Cuts at: 8454 8454
Size: 10864
KasI G'GCGC_C
Cuts at: 3302 3302
Size: 10864
KpnI G_GTAC'C
Cuts at: 2185 8189 2185
Size: 6004 4860
MfeI C'AATT_G
Cuts at: 8463 8463
Size: 10864
MluI A'CGCG_T
Cuts at: 10740 10740
Size: 10864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3369 4669 7320 9978 10162 1305
Size: 2064 1300 2651 2658 184 2007
Fragments arranged by size:
2658 2651 2064 2007 1300 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 5706 6809 7928 10
Size: 54 1425 343 357 3517 1103 1119 2946
Fragments arranged by size:
3517 2946 1425 1119 1103 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 2930 3286 4886 5015 6002 6036
Size: 648 1132 632 356 1600 129 987 34
Cuts at: 6036 6938 7052 7157 8060 8159 8822 9181 9340
Size: 902 114 105 903 99 663 359 159
Cuts at: 9340 518
Size: 2042
Fragments arranged by size:
2042 1600 1132 987 903 902 663 648
632 359 356 159 129 114 105 99
34
MspA1I CmG'CkG
Cuts at: 1606 2153 2318 2778 2817 2871 3045 3838 4467
Size: 547 165 460 39 54 174 793 629
Cuts at: 4467 4574 4602 5775 6994 7474 7889 8280 8957
Size: 107 28 1173 1219 480 415 391 677
Cuts at: 8957 10105 10350 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1219 1173 1148 793 677 629 547
480 460 415 391 245 174 165 107
54 39 28
NaeI GCC'GGC
Cuts at: 2332 2722 2795 5712 5818 7948 2332
Size: 390 73 2917 106 2130 5248
Fragments arranged by size:
5248 2917 2130 390 106 73
NarI GG'CG_CC
Cuts at: 3303 3303
Size: 10864
NcoI C'CATG_G
Cuts at: 513 1065 4974 6009 513
Size: 552 3909 1035 5368
Fragments arranged by size:
5368 3909 1035 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10864
NgoMIV G'CCGG_C
Cuts at: 2330 2720 2793 5710 5816 7946 2330
Size: 390 73 2917 106 2130 5248
Fragments arranged by size:
5248 2917 2130 390 106 73
NheI G'CTAG_C
Cuts at: 1052 5615 1052
Size: 4563 6301
NotI GC'GGCC_GC
Cuts at: 8311 8311
Size: 10864
NsiI A_TGCA'T
Cuts at: 4013 4013
Size: 10864
NspI r_CATG'y
Cuts at: 1132 1229 3877 4011 4181 4891 6491 7162 7457
Size: 97 2648 134 170 710 1600 671 295
Cuts at: 7457 8222 9767 1132
Size: 765 1545 2229
Fragments arranged by size:
2648 2229 1600 1545 765 710 671 295
170 134 97
PasI CC'CwG_GG
Cuts at: 1348 6955 6984 7877 1348
Size: 5607 29 893 4335
Fragments arranged by size:
5607 4335 893 29
PciI A'CATG_T
Cuts at: 7158 9763 7158
Size: 2605 8259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2731 2778 3045 3615 5506 1108
Size: 1623 47 267 570 1891 6466
Fragments arranged by size:
6466 1891 1623 570 267 47
PfoI T'CCnGG_A
Cuts at: 2031 2688 4689 7193 2031
Size: 657 2001 2504 5702
Fragments arranged by size:
5702 2504 2001 657
PmeI GTTT'AAAC
Cuts at: 8168 8168
Size: 10864
PmlI CAC'GTG
Cuts at: 6090 6351 6639 6090
Size: 261 288 10315
Fragments arranged by size:
10315 288 261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7220 7252 8933 8965 10446 10478 7220
Size: 32 1681 32 1481 32 7606
Fragments arranged by size:
7606 1681 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3675 5579 6567 7145 3675
Size: 1904 988 578 7394
Fragments arranged by size:
7394 1904 988 578
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10864
PsiI TTA'TAA
Cuts at: 8434 8434
Size: 10864
PspOMI G'GGCC_C
Cuts at: 3327 4967 5228 5298 6958 7881 8047 3327
Size: 1640 261 70 1660 923 166 6144
Fragments arranged by size:
6144 1660 1640 923 261 166 70
PstI C_TGCA'G
Cuts at: 830 2253 3584 3969 5049 6507 7479 8216 830
Size: 1423 1331 385 1080 1458 972 737 3478
Fragments arranged by size:
3478 1458 1423 1331 1080 972 737 385
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10864
PvuII CAG'CTG
Cuts at: 3838 4602 6994 7474 3838
Size: 764 2392 480 7228
Fragments arranged by size:
7228 2392 764 480
RsrII CG'GwC_CG
Cuts at: 2859 7642 2859
Size: 4783 6081
SacI G_AGCT'C
Cuts at: 3962 4935 3962
Size: 973 9891
SacII CC_GC'GG
Cuts at: 2154 2319 5776 2154
Size: 165 3457 7242
Fragments arranged by size:
7242 3457 165
SalI G'TCGA_C
Cuts at: 1936 8206 1936
Size: 6270 4594
SanDI GG'GwC_CC
Cuts at: 3675 3675
Size: 10864
SapI GCTCTTCn'nnn_
Cuts at: 3131 5753 6836 3131
Size: 2622 1083 7159
Fragments arranged by size:
7159 2622 1083
SbfI CC_TGCA'GG
Cuts at: 6507 8216 6507
Size: 1709 9155
ScaI AGT'ACT
Cuts at: 6263 9112 10824 6263
Size: 2849 1712 6303
Fragments arranged by size:
6303 2849 1712
SfcI C'TryA_G
Cuts at: 826 1045 2249 3580 3965 5045 5080 5491 6257
Size: 219 1204 1331 385 1080 35 411 766
Cuts at: 6257 6503 7351 7475 8212 9347 10028 10219 826
Size: 246 848 124 737 1135 681 191 1471
Fragments arranged by size:
1471 1331 1204 1135 1080 848 766 737
681 411 385 246 219 191 124 35
SfoI GGC'GCC
Cuts at: 3304 3304
Size: 10864
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10864
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10864
SmaI CCC'GGG
Cuts at: 1872 3268 5698 7442 8191 1872
Size: 1396 2430 1744 749 4545
Fragments arranged by size:
4545 2430 1744 1396 749
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2783 3212 3680 3986 4565 5171
Size: 197 926 840 429 468 306 579 606
Cuts at: 5171 6338 6750 7237 8087 8968 9869 10131 820
Size: 1167 412 487 850 881 901 262 1553
Fragments arranged by size:
1553 1167 926 901 881 850 840 606
579 487 468 429 412 306 262 197
SnaBI TAC'GTA
Cuts at: 493 8655 493
Size: 8162 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10864
SphI G_CATG'C
Cuts at: 1132 4181 6491 7457 8222 1132
Size: 3049 2310 966 765 3774
Fragments arranged by size:
3774 3049 2310 966 765
SrfI GCCC'GGGC
Cuts at: 7442 7442
Size: 10864
SspI AAT'ATT
Cuts at: 5 52 8788 5
Size: 47 8736 2081
Fragments arranged by size:
8736 2081 47
StuI AGG'CCT
Cuts at: 1517 7268 7730 7787 1517
Size: 5751 462 57 4594
Fragments arranged by size:
5751 4594 462 57
StyI C'CwwG_G
Cuts at: 513 1065 3297 4161 4182 4527 4974 5562 5909
Size: 552 2232 864 21 345 447 588 347
Cuts at: 5909 5976 6009 6561 7860 8303 513
Size: 67 33 552 1299 443 3074
Fragments arranged by size:
3074 2232 1299 864 588 552 552 447
443 347 345 67 33 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2876 3535 4174 5047 5531 5785 6485
Size: 1255 1296 659 639 873 484 254 700
Cuts at: 6485 6751 7137 7639 7835 7890 8005 8891 8908
Size: 266 386 502 196 55 115 886 17
Cuts at: 8908 9061 9246 9691 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1296 1255 886 873 700 659 639
502 484 445 386 266 254 196 185
153 115 55 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2550 3378 4875
Size: 274 80 33 51 1443 573 828 1497
Cuts at: 4875 4890 5112 5673 6261 7100 9110 10822 96
Size: 15 222 561 588 839 2010 1712 138
Fragments arranged by size:
2010 1712 1497 1443 839 828 588 573
561 274 222 138 80 51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3510 3542 3710 3742 4995 5027 6205 6237 6464
Size: 32 168 32 1253 32 1178 32 227
Cuts at: 6464 6496 8017 8049 10824 10856 3510
Size: 32 1521 32 2775 32 3518
Fragments arranged by size:
3518 2775 1521 1253 1178 227 168 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2498 4484 4502 4544 1316
Size: 1182 1986 18 42 7636
Fragments arranged by size:
7636 1986 1182 42 18
XbaI T'CTAG_A
Cuts at: 8200 8200
Size: 10864
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6382 6817 7798 7858 6382
Size: 435 981 60 9388
Fragments arranged by size:
9388 981 435 60
XhoI C'TCGA_G
Cuts at: 1943 8087 1943
Size: 6144 4720
XmaI C'CCGG_G
Cuts at: 1870 3266 5696 7440 8189 1870
Size: 1396 2430 1744 749 4545
Fragments arranged by size:
4545 2430 1744 1396 749
XmnI GAAnn'nnTTC
Cuts at: 6779 8993 6779
Size: 2214 8650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8672 276
Size: 53 83 186 936 7138 2468
Fragments arranged by size:
7138 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BstZ17I Bsu36I EcoRV FalI FspAI
NruI PacI PspXI PsrI SexAI SfiI SwaI