Restriction map for
pFN21AA1122
(Circular) (Six-base) MAPSORT of: pFN21AA1122.seq Check: 5152 from: 1 to: 7789
pFN21AA1122 7789 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2597 2597
Size: 7789
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5599 278
Size: 53 83 186 936 4063 2468
Fragments arranged by size:
4063 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5132 1937
Size: 3195 4594
Acc65I G'GTAC_C
Cuts at: 4698 5110 4698
Size: 412 7377
AclI AA'CG_TT
Cuts at: 1563 5916 6289 1563
Size: 4353 373 3063
Fragments arranged by size:
4353 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2145 2160 2406 2474 2628 3923 4812 5852
Size: 513 15 246 68 154 1295 889 1040
Cuts at: 5852 7236 7504 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1295 1040 889 513 268 246
154 68 15
AflII C'TTAA_G
Cuts at: 820 1017 2537 820
Size: 197 1520 6072
Fragments arranged by size:
6072 1520 197
AflIII A'CryG_T
Cuts at: 1203 4035 4501 6688 7665 1203
Size: 2832 466 2187 977 1327
Fragments arranged by size:
2832 2187 1327 977 466
AgeI A'CCGG_T
Cuts at: 6620 6620
Size: 7789
AhdI GACnn_n'nnGTC
Cuts at: 6518 6518
Size: 7789
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7789
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7789
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 7757
AlwNI CAG_nnn'CTG
Cuts at: 3095 5532 7104 3095
Size: 2437 1572 3780
Fragments arranged by size:
3780 2437 1572
ApaI G_GGCC'C
Cuts at: 2619 2619
Size: 7789
ApaLI G'TGCA_C
Cuts at: 5846 7002 5846
Size: 1156 6633
ApoI r'AATT_y
Cuts at: 1493 2666 2789 2996 3168 4018 4574 5098 5324
Size: 1173 123 207 172 850 556 524 226
Cuts at: 5324 5476 7585 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1173 850 556 524 226 207
172 152 123
AseI AT'TA_AT
Cuts at: 160 1032 3945 4530 6343 160
Size: 872 2913 585 1813 1606
Fragments arranged by size:
2913 1813 1606 872 585
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7789
AvaI C'yCGr_G
Cuts at: 1870 1943 5114 1870
Size: 73 3171 4545
Fragments arranged by size:
4545 3171 73
AvrII C'CTAG_G
Cuts at: 3580 3580
Size: 7789
BamHI G'GATC_C
Cuts at: 5119 5486 5119
Size: 367 7422
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3705 4698 5110 6565 618
Size: 325 217 627 1918 993 412 1455 1842
Fragments arranged by size:
1918 1842 1455 993 627 412 325 217
BanII G_rGCy'C
Cuts at: 1391 2619 3917 5108 1391
Size: 1228 1298 1191 4072
Fragments arranged by size:
4072 1298 1228 1191
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2280 2457 2475 2892 3078 3399 928
Size: 971 381 177 18 417 186 321 5318
Fragments arranged by size:
5318 971 417 381 321 186 177 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5980 6014 1903
Size: 34 4043 34 3678
Fragments arranged by size:
4043 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5681 6891 5681
Size: 1210 6579
BclI T'GATC_A
Cuts at: 1548 2677 1548
Size: 1129 6660
BfrBI ATG'CAT
Cuts at: 4593 4593
Size: 7789
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6400 136
Size: 107 122 71 631 605 4728 1525
Fragments arranged by size:
4728 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7784 7784
Size: 7789
BlpI GC'TnA_GC
Cuts at: 5206 5206
Size: 7789
Bme1580I G_kGCm'C
Cuts at: 1790 2619 3710 5850 7006 1790
Size: 829 1091 2140 1156 2573
Fragments arranged by size:
2573 2140 1156 1091 829
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7789
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5520 6478 442
Size: 416 53 83 399 24 4103 958 1753
Fragments arranged by size:
4103 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7789
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2843
Size: 7789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2202 6449 1239
Size: 963 4247 2579
Fragments arranged by size:
4247 2579 963
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2813 3479 3726 4172 4385 5914 6779 7077 2813
Size: 666 247 446 213 1529 865 298 3525
Fragments arranged by size:
3525 1529 865 666 446 298 247 213
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6452 882
Size: 617 4953 2219
Fragments arranged by size:
4953 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2423 5580 493
Size: 711 1219 3157 2702
Fragments arranged by size:
3157 2702 1219 711
BsaBI GATnn'nnATC
Cuts at: 7783 7783
Size: 7789
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5596 5978 6613 275
Size: 53 83 186 936 4063 382 635 1451
Fragments arranged by size:
4063 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6222 6620 6894 7041 7452 6222
Size: 398 274 147 411 6559
Fragments arranged by size:
6559 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3269 3299 1178
Size: 30 165 30 1866 30 5668
Fragments arranged by size:
5668 1866 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 1186
Size: 958 6831
BseYI C'CCAG_C
Cuts at: 2088 6992 7488 2088
Size: 4904 496 2389
Fragments arranged by size:
4904 2389 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7216
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5239 6000 7028 1540
Size: 275 186 3238 761 1028 2301
Fragments arranged by size:
3238 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5850 5935 7006 5850
Size: 85 1071 6633
Fragments arranged by size:
6633 1071 85
BsmI GAATG_Cn'
Cuts at: 2140 5300 5393 2140
Size: 3160 93 4536
Fragments arranged by size:
4536 3160 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2619 3710 3917 5108 5850 5935 7006
Size: 399 829 1091 207 1191 742 85 1071
Cuts at: 7006 1391
Size: 2174
Fragments arranged by size:
2174 1191 1091 1071 829 742 399 207
85
BspEI T'CCGG_A
Cuts at: 7452 7452
Size: 7789
BspHI T'CATG_A
Cuts at: 4332 5678 7408 4332
Size: 1346 1730 4713
Fragments arranged by size:
4713 1730 1346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2597 5144 844
Size: 1753 2547 3489
Fragments arranged by size:
3489 2547 1753
BsrBI CCG'CTC
Cuts at: 1394 1823 5676 1394
Size: 429 3853 3507
Fragments arranged by size:
3853 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3600 4236 4333 6284 6458 65
Size: 1183 2352 636 97 1951 174 1396
Fragments arranged by size:
2352 1951 1396 1183 636 174 97
BsrFI r'CCGG_y
Cuts at: 1700 1810 6433 6620 1700
Size: 110 4623 187 2869
Fragments arranged by size:
4623 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2847 3982 96
Size: 2751 1135 3903
Fragments arranged by size:
3903 2751 1135
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7789
BssSI C'ACGA_G
Cuts at: 5849 6861 5849
Size: 1012 6777
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2579 794
Size: 1785 6004
BstBI TT'CG_AA
Cuts at: 4494 6593 4494
Size: 2099 5690
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7789
BstXI CCAn_nnnn'nTGG
Cuts at: 4657 4657
Size: 7789
BstYI r'GATC_y
Cuts at: 1972 5119 5486 5871 5888 7340 7426 7438 7784
Size: 3147 367 385 17 1452 86 12 346
Cuts at: 7784 1972
Size: 1977
Fragments arranged by size:
3147 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2415 513
Size: 552 1350 5887
Fragments arranged by size:
5887 1350 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4358 487
Size: 683 56 3132 3918
Fragments arranged by size:
3918 3132 683 56
BtsI GCAGTG_nn'
Cuts at: 5305 6099 6119 5305
Size: 794 20 6975
Fragments arranged by size:
6975 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7789
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7754
DraI TTT'AAA
Cuts at: 5093 5093
Size: 7789
DraIII CAC_nnn'GTG
Cuts at: 2915 4442 2915
Size: 1527 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6796 809
Size: 507 5480 1802
Fragments arranged by size:
5480 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4353 5236 6125
Size: 54 1425 50 275 18 2523 883 889
Cuts at: 6125 7754 8
Size: 1629 43
Fragments arranged by size:
2523 1629 1425 889 883 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5236 1537
Size: 275 3424 4090
Fragments arranged by size:
4090 3424 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2388 4162 4173 5719 1353
Size: 768 267 1774 11 1546 3423
Fragments arranged by size:
3423 1774 1546 768 267 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6376 6750 6896 387
Size: 1064 138 4787 374 146 1280
Fragments arranged by size:
4787 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2145 2160 2202 2406 2474 2628 3923
Size: 393 513 15 42 204 68 154 1295
Cuts at: 3923 4812 5852 6449 7236 7504 1239
Size: 889 1040 597 787 268 1524
Fragments arranged by size:
1524 1295 1040 889 787 597 513 393
268 204 154 68 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3623 3623
Size: 7789
EcoO109I rG'GnC_Cy
Cuts at: 3721 3721
Size: 7789
EcoRI G'AATT_C
Cuts at: 5098 5476 5098
Size: 378 7411
EcoRV GAT'ATC
Cuts at: 4663 4774 4663
Size: 111 7678
FspI TGC'GCA
Cuts at: 6295 6295
Size: 7789
HaeII r_GCGC'y
Cuts at: 1678 2066 6936 1678
Size: 388 4870 2531
Fragments arranged by size:
4870 2531 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2666 2698 6430
Size: 32 135 32 809 32 253 32 3732
Cuts at: 6430 6462 6504 6536 1373
Size: 32 42 32 2626
Fragments arranged by size:
3732 2626 809 253 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5133 5379 669
Size: 988 281 3195 246 3079
Fragments arranged by size:
3195 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2856 748
Size: 2108 5681
HpaI GTT'AAC
Cuts at: 5379 5379
Size: 7789
KpnI G_GTAC'C
Cuts at: 4702 5114 4702
Size: 412 7377
MfeI C'AATT_G
Cuts at: 2574 3195 5388 2574
Size: 621 2193 4975
Fragments arranged by size:
4975 2193 621
MluI A'CGCG_T
Cuts at: 7665 7665
Size: 7789
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4215 6903 7087 1305
Size: 2910 2688 184 2007
Fragments arranged by size:
2910 2688 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4355 10
Size: 54 1425 343 2523 3444
Fragments arranged by size:
3444 2523 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3293 3543 4319 5747 6106 6265 518
Size: 648 2127 250 776 1428 359 159 2042
Fragments arranged by size:
2127 2042 1428 776 648 359 250 159
MspA1I CmG'CkG
Cuts at: 1606 4391 5205 5882 7030 7275 1606
Size: 2785 814 677 1148 245 2120
Fragments arranged by size:
2785 2120 1148 814 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2415 513
Size: 552 1350 5887
Fragments arranged by size:
5887 1350 552
NdeI CA'TA_TG
Cuts at: 387 2653 3897 387
Size: 2266 1244 4279
Fragments arranged by size:
4279 2266 1244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7789
NotI GC'GGCC_GC
Cuts at: 5236 5236
Size: 7789
NsiI A_TGCA'T
Cuts at: 4595 4595
Size: 7789
NspI r_CATG'y
Cuts at: 1132 1229 4039 4136 4505 5147 6692 1132
Size: 97 2810 97 369 642 1545 2229
Fragments arranged by size:
2810 2229 1545 642 369 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7789
PciI A'CATG_T
Cuts at: 4035 4501 6688 4035
Size: 466 2187 5136
Fragments arranged by size:
5136 2187 466
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7789
PmeI GTTT'AAAC
Cuts at: 5093 5093
Size: 7789
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 5858 5890 7371 7403 3269
Size: 32 2557 32 1481 32 3655
Fragments arranged by size:
3655 2557 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3721 3721
Size: 7789
PshAI GACnn'nnGTC
Cuts at: 1613 3846 1613
Size: 2233 5556
PsiI TTA'TAA
Cuts at: 3371 4148 4872 4934 5359 3371
Size: 777 724 62 425 5801
Fragments arranged by size:
5801 777 724 425 62
PspOMI G'GGCC_C
Cuts at: 2615 2615
Size: 7789
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4488 4520 4488
Size: 32 7757
PstI C_TGCA'G
Cuts at: 830 2734 5141 830
Size: 1904 2407 3478
Fragments arranged by size:
3478 2407 1904
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7789
PvuII CAG'CTG
Cuts at: 4391 4391
Size: 7789
SalI G'TCGA_C
Cuts at: 1936 5131 1936
Size: 3195 4594
SbfI CC_TGCA'GG
Cuts at: 5141 5141
Size: 7789
ScaI AGT'ACT
Cuts at: 2826 4926 6037 7749 2826
Size: 2100 1111 1712 2866
Fragments arranged by size:
2866 2100 1712 1111
SfcI C'TryA_G
Cuts at: 826 1045 2730 3124 3672 5016 5137 6272 6953
Size: 219 1685 394 548 1344 121 1135 681
Cuts at: 6953 7144 826
Size: 191 1471
Fragments arranged by size:
1685 1471 1344 1135 681 548 394 219
191 121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7789
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7789
SmaI CCC'GGG
Cuts at: 1872 5116 1872
Size: 3244 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2537 2828 3494 3741 4187 4400
Size: 197 926 594 291 666 247 446 213
Cuts at: 4400 5893 6794 7056 820
Size: 1493 901 262 1553
Fragments arranged by size:
1553 1493 926 901 666 594 446 291
262 247 213 197
SnaBI TAC'GTA
Cuts at: 493 5580 493
Size: 5087 2702
SpeI A'CTAG_T
Cuts at: 152 4927 152
Size: 4775 3014
SphI G_CATG'C
Cuts at: 1132 5147 1132
Size: 4015 3774
SspI AAT'ATT
Cuts at: 5 52 4382 5713 5
Size: 47 4330 1331 2081
Fragments arranged by size:
4330 2081 1331 47
StuI AGG'CCT
Cuts at: 1517 2406 1517
Size: 889 6900
StyI C'CwwG_G
Cuts at: 513 1065 2401 2415 3580 4948 5228 513
Size: 552 1336 14 1165 1368 280 3074
Fragments arranged by size:
3074 1368 1336 1165 552 280 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5816 5833 5986 6171 6616 325
Size: 1255 4236 17 153 185 445 1498
Fragments arranged by size:
4236 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2770 2824 2847
Size: 274 80 33 51 1443 793 54 23
Cuts at: 2847 3982 4504 4924 6035 7747 96
Size: 1135 522 420 1111 1712 138
Fragments arranged by size:
1712 1443 1135 1111 793 522 420 274
138 80 54 51 33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7749 7781 7749
Size: 32 7757
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7789
XbaI T'CTAG_A
Cuts at: 5125 5125
Size: 7789
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3607 3607
Size: 7789
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7789
XmaI C'CCGG_G
Cuts at: 1870 5114 1870
Size: 3244 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 2345 2508 4072 4182 4737 5918 2012
Size: 333 163 1564 110 555 1181 3883
Fragments arranged by size:
3883 1564 1181 555 333 163 110
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5597 276
Size: 53 83 186 936 4063 2468
Fragments arranged by size:
4063 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI
BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI