Restriction map for
pFN21AA1119
(Circular) (Six-base) MAPSORT of: pFN21AA1119.seq Check: 3871 from: 1 to: 10252
pFN21AA1119 10252 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3181 4440 5386 6674 3181
Size: 1259 946 1288 6759
Fragments arranged by size:
6759 1288 1259 946
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6647 7116 8062 278
Size: 53 83 186 936 5111 469 946 2468
Fragments arranged by size:
5111 2468 946 936 469 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3072 7595 1937
Size: 1135 4523 4594
Fragments arranged by size:
4594 4523 1135
Acc65I G'GTAC_C
Cuts at: 2697 5205 7573 2697
Size: 2508 2368 5376
Fragments arranged by size:
5376 2508 2368
AclI AA'CG_TT
Cuts at: 1563 8379 8752 1563
Size: 6816 373 3063
Fragments arranged by size:
6816 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3764 4385 4733 4910 5468 5627 5663 6131
Size: 2132 621 348 177 558 159 36 468
Cuts at: 6131 6308 6707 8315 9699 9967 1632
Size: 177 399 1608 1384 268 1917
Fragments arranged by size:
2132 1917 1608 1384 621 558 468 399
348 268 177 177 159 36
AfeI AGC'GCT
Cuts at: 6929 6929
Size: 10252
AflII C'TTAA_G
Cuts at: 820 1017 6752 820
Size: 197 5735 4320
Fragments arranged by size:
5735 4320 197
AflIII A'CryG_T
Cuts at: 1203 6525 7455 9151 10128 1203
Size: 5322 930 1696 977 1327
Fragments arranged by size:
5322 1696 1327 977 930
AgeI A'CCGG_T
Cuts at: 4119 9083 4119
Size: 4964 5288
AhdI GACnn_n'nnGTC
Cuts at: 2369 8981 2369
Size: 6612 3640
AleI CACnn'nnGTG
Cuts at: 1166 7197 1166
Size: 6031 4221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2195 3246 4994 5091 7151 1184
Size: 1011 1051 1748 97 2060 4285
Fragments arranged by size:
4285 2060 1748 1051 1011 97
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7212 7244 7212
Size: 32 10220
AlwNI CAG_nnn'CTG
Cuts at: 4044 4651 6061 6484 6787 7246 7435 7995 9567
Size: 607 1410 423 303 459 189 560 1572
Cuts at: 9567 4044
Size: 4729
Fragments arranged by size:
4729 1572 1410 607 560 459 423 303
189
ApaI G_GGCC'C
Cuts at: 4489 4990 4489
Size: 501 9751
ApaLI G'TGCA_C
Cuts at: 2032 4904 6569 8309 9465 2032
Size: 2872 1665 1740 1156 2819
Fragments arranged by size:
2872 2819 1740 1665 1156
ApoI r'AATT_y
Cuts at: 1493 2822 5138 6182 6757 7329 7358 7535 7561
Size: 1329 2316 1044 575 572 29 177 26
Cuts at: 7561 7787 7939 10048 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2316 2109 1697 1329 1044 575 572 226
177 152 29 26
AseI AT'TA_AT
Cuts at: 160 1032 2334 5862 8806 160
Size: 872 1302 3528 2944 1606
Fragments arranged by size:
3528 2944 1606 1302 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10252
AvaI C'yCGr_G
Cuts at: 1870 1943 4542 5768 6101 7577 1870
Size: 73 2599 1226 333 1476 4545
Fragments arranged by size:
4545 2599 1476 1226 333 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2299 2332 2299
Size: 33 10219
BamHI G'GATC_C
Cuts at: 2046 7582 7949 2046
Size: 5536 367 4349
Fragments arranged by size:
5536 4349 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2697 5205 7573 9028 618
Size: 325 217 627 910 2508 2368 1455 1842
Fragments arranged by size:
2508 2368 1842 1455 910 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 3580 4152 4489 4922 4990 5769 6366
Size: 631 1558 572 337 433 68 779 597
Cuts at: 6366 7571 1391
Size: 1205 4072
Fragments arranged by size:
4072 1558 1205 779 631 597 572 433
337 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2516 2746 2807 2930 5434 928
Size: 971 617 230 61 123 2504 5746
Fragments arranged by size:
5746 2504 971 617 230 123 61
BbvCI CC'TCA_GC
Cuts at: 2910 5042 5663 7307 7428 2910
Size: 2132 621 1644 121 5734
Fragments arranged by size:
5734 2132 1644 621 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8443 8477 1903
Size: 34 6506 34 3678
Fragments arranged by size:
6506 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8144 9354 8144
Size: 1210 9042
BclI T'GATC_A
Cuts at: 1548 3195 7071 1548
Size: 1647 3876 4729
Fragments arranged by size:
4729 3876 1647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2664 4493 5921
Size: 107 122 71 631 605 992 1829 1428
Cuts at: 5921 8863 136
Size: 2942 1525
Fragments arranged by size:
2942 1829 1525 1428 992 631 605 122
107 71
BglII A'GATC_T
Cuts at: 4239 5004 5587 6807 10247 4239
Size: 765 583 1220 3440 4244
Fragments arranged by size:
4244 3440 1220 765 583
BlpI GC'TnA_GC
Cuts at: 2074 3030 4425 4738 6042 6096 7669 2074
Size: 956 1395 313 1304 54 1573 4657
Fragments arranged by size:
4657 1573 1395 1304 956 313 54
Bme1580I G_kGCm'C
Cuts at: 1790 2036 4489 4908 4990 6494 6501 6573 8313
Size: 246 2453 419 82 1504 7 72 1740
Cuts at: 8313 9469 1790
Size: 1156 2573
Fragments arranged by size:
2573 2453 1740 1504 1156 419 246 82
72 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10252
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3229 3583 7983
Size: 416 53 83 399 24 1812 354 4400
Cuts at: 7983 8941 442
Size: 958 1753
Fragments arranged by size:
4400 1812 1753 958 416 399 354 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10252
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3700 4753 3700
Size: 1053 9199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2306 2520 3374 3443 3586 3719 4211
Size: 911 156 214 854 69 143 133 492
Cuts at: 4211 4292 4445 5084 5207 5274 5375 5435 5495
Size: 81 153 639 123 67 101 60 60
Cuts at: 5495 5528 5642 5686 6056 6083 6437 6919 8912
Size: 33 114 44 370 27 354 482 1993
Cuts at: 8912 1239
Size: 2579
Fragments arranged by size:
2579 1993 911 854 639 492 482 370
354 214 156 153 143 133 123 114
101 81 69 67 60 60 44 33
27
Bpu10I CC'TnA_GC
Cuts at: 2234 2910 3077 5042 5663 7307 7428 2234
Size: 676 167 1965 621 1644 121 5058
Fragments arranged by size:
5058 1965 1644 676 621 167 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3272 4679 5732 6074 6548 7184 8377 9242 9540
Size: 1407 1053 342 474 636 1193 865 298
Cuts at: 9540 3272
Size: 3984
Fragments arranged by size:
3984 1407 1193 1053 865 636 474 342
298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3155 3932 8915 882
Size: 617 1656 777 4983 2219
Fragments arranged by size:
4983 2219 1656 777 617
BsaAI yAC'GTr
Cuts at: 493 1204 4615 8043 493
Size: 711 3411 3428 2702
Fragments arranged by size:
3428 3411 2702 711
BsaBI GATnn'nnATC
Cuts at: 3737 4067 10246 3737
Size: 330 6179 3743
Fragments arranged by size:
6179 3743 330
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5016 6644 7113 8059
Size: 53 83 186 936 3483 1628 469 946
Cuts at: 8059 8441 9076 275
Size: 382 635 1451
Fragments arranged by size:
3483 1628 1451 946 936 635 469 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4119 4296 4686 6450 6921 8685 9083 9357 9504
Size: 177 390 1764 471 1764 398 274 147
Cuts at: 9504 9915 4119
Size: 411 4456
Fragments arranged by size:
4456 1764 1764 471 411 398 390 274
177 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3584 3614 1178
Size: 30 165 30 2181 30 7816
Fragments arranged by size:
7816 2181 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2892 2936 4967 5159 5204 5207 5777 5798
Size: 1706 44 2031 192 45 3 570 21
Cuts at: 5798 6086 6089 6131 6434 6922 7181 1186
Size: 288 3 42 303 488 259 4257
Fragments arranged by size:
4257 2031 1706 570 488 303 288 259
192 45 44 42 21 3 3
BseYI C'CCAG_C
Cuts at: 3514 7239 7314 7516 9455 9951 3514
Size: 3725 75 202 1939 496 3815
Fragments arranged by size:
3815 3725 1939 496 202 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2868 3260 4049 4095 5522 1163
Size: 573 1132 392 789 46 1427 5893
Fragments arranged by size:
5893 1427 1132 789 573 392 46
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7702 8463 9491 1540
Size: 275 186 5701 761 1028 2301
Fragments arranged by size:
5701 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2036 3111 3126 3261 3888 4152 4431 4461
Size: 14 1075 15 135 627 264 279 30
Cuts at: 4461 4590 4908 5769 5793 6366 6573 7130 8313
Size: 129 318 861 24 573 207 557 1183
Cuts at: 8313 8398 9469 2022
Size: 85 1071 2805
Fragments arranged by size:
2805 1183 1075 1071 861 627 573 557
318 279 264 207 135 129 85 30
24 15 14
BsmI GAATG_Cn'
Cuts at: 3952 5587 6885 6997 7763 7856 3952
Size: 1635 1298 112 766 93 6348
Fragments arranged by size:
6348 1635 1298 766 112 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4052 4763 4052
Size: 711 9541
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2036 3111 3126 3261 3580 3888
Size: 399 232 14 1075 15 135 319 308
Cuts at: 3888 4152 4431 4461 4489 4590 4908 4922 4990
Size: 264 279 30 28 101 318 14 68
Cuts at: 4990 5769 5793 6366 6494 6501 6573 7130 7571
Size: 779 24 573 128 7 72 557 441
Cuts at: 7571 8313 8398 9469 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1075 1071 779 742 573 557 441
399 319 318 308 279 264 232 135
128 101 85 72 68 30 28 24
15 14 14 7
BspEI T'CCGG_A
Cuts at: 4296 4686 6450 9915 4296
Size: 390 1764 3465 4633
Fragments arranged by size:
4633 3465 1764 390
BspHI T'CATG_A
Cuts at: 2245 3781 4311 5172 6714 8141 9871 2245
Size: 1536 530 861 1542 1427 1730 2626
Fragments arranged by size:
2626 1730 1542 1536 1427 861 530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3181 4170 4440 5386 6364 6674 7036 7104
Size: 2337 989 270 946 978 310 362 68
Cuts at: 7104 7607 844
Size: 503 3489
Fragments arranged by size:
3489 2337 989 978 946 503 362 310
270 68
BsrBI CCG'CTC
Cuts at: 1394 1823 4448 4737 4885 7417 8139 1394
Size: 429 2625 289 148 2532 722 3507
Fragments arranged by size:
3507 2625 2532 722 429 289 148
BsrDI GCAATG_nn'
Cuts at: 65 1248 2650 8747 8921 65
Size: 1183 1402 6097 174 1396
Fragments arranged by size:
6097 1402 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2810 3594 4119 5837 6914 8896 9083
Size: 110 1000 784 525 1718 1077 1982 187
Cuts at: 9083 1700
Size: 2869
Fragments arranged by size:
2869 1982 1718 1077 1000 784 525 187
110
BsrGI T'GTAC_A
Cuts at: 96 2164 96
Size: 2068 8184
BssHII G'CGCG_C
Cuts at: 1925 3203 1925
Size: 1278 8974
BssSI C'ACGA_G
Cuts at: 5759 8312 9324 5759
Size: 2553 1012 6687
Fragments arranged by size:
6687 2553 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3087 6577 794
Size: 2293 3490 4469
Fragments arranged by size:
4469 3490 2293
BstBI TT'CG_AA
Cuts at: 3833 4066 9056 3833
Size: 233 4990 5029
Fragments arranged by size:
5029 4990 233
BstEII G'GTnAC_C
Cuts at: 1184 3148 1184
Size: 1964 8288
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 4095 6034 2011
Size: 2084 1939 6229
Fragments arranged by size:
6229 2084 1939
BstYI r'GATC_y
Cuts at: 1972 2046 3450 4239 5004 5587 6807 7582 7949
Size: 74 1404 789 765 583 1220 775 367
Cuts at: 7949 8334 8351 9803 9889 9901 10247 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1404 1220 789 775 765 583
385 367 346 86 74 17 12
BstZ17I GTA'TAC
Cuts at: 3073 3073
Size: 10252
Bsu36I CC'TnA_GG
Cuts at: 4721 4940 5798 4721
Size: 219 858 9175
Fragments arranged by size:
9175 858 219
BtgI C'CryG_G
Cuts at: 513 1065 4872 5427 5673 5707 7215 7497 513
Size: 552 3807 555 246 34 1508 282 3268
Fragments arranged by size:
3807 3268 1508 555 552 282 246 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3122 487
Size: 683 56 1896 7617
Fragments arranged by size:
7617 1896 683 56
BtsI GCAGTG_nn'
Cuts at: 4032 6726 7086 7768 8562 8582 4032
Size: 2694 360 682 794 20 5702
Fragments arranged by size:
5702 2694 794 682 360 20
ClaI AT'CG_AT
Cuts at: 1554 4806 1554
Size: 3252 7000
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10217
DraI TTT'AAA
Cuts at: 2827 5976 7335 7556 2827
Size: 3149 1359 221 5523
Fragments arranged by size:
5523 3149 1359 221
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5438 9259 809
Size: 507 4122 3821 1802
Fragments arranged by size:
4122 3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2389 2454 2562
Size: 54 1425 50 275 18 559 65 108
Cuts at: 2562 3901 4228 5676 7699 8588 10217 8
Size: 1339 327 1448 2023 889 1629 43
Fragments arranged by size:
2023 1629 1448 1425 1339 889 559 327
275 108 65 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7699 1537
Size: 275 5887 4090
Fragments arranged by size:
5887 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2577 3720 4136 5453 5507 5603 7067 8182
Size: 1224 1143 416 1317 54 96 1464 1115
Cuts at: 8182 1353
Size: 3423
Fragments arranged by size:
3423 1464 1317 1224 1143 1115 416 96
54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5317 5561 6971 8839 9213 9359
Size: 1064 138 3728 244 1410 1868 374 146
Cuts at: 9359 387
Size: 1280
Fragments arranged by size:
3728 1868 1410 1280 1064 374 244 146
138
EcoICRI GAG'CTC
Cuts at: 2020 4150 5767 6364 2020
Size: 2130 1617 597 5908
Fragments arranged by size:
5908 2130 1617 597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2306 2520 3374 3443 3586 3719
Size: 393 518 156 214 854 69 143 133
Cuts at: 3719 3764 4211 4292 4385 4445 4733 4910 5084
Size: 45 447 81 93 60 288 177 174
Cuts at: 5084 5207 5274 5375 5435 5468 5495 5528 5627
Size: 123 67 101 60 33 27 33 99
Cuts at: 5627 5642 5663 5686 6056 6083 6131 6308 6437
Size: 15 21 23 370 27 48 177 129
Cuts at: 6437 6707 6919 8315 8912 9699 9967 1239
Size: 270 212 1396 597 787 268 1524
Fragments arranged by size:
1524 1396 854 787 597 518 447 393
370 288 270 268 214 212 177 177
174 156 143 133 129 123 101 99
93 81 69 67 60 60 48 45
33 33 27 27 23 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3188 4202 4501 5219 5393 5417 6113 3188
Size: 1014 299 718 174 24 696 7327
Fragments arranged by size:
7327 1014 718 696 299 174 24
EcoO109I rG'GnC_Cy
Cuts at: 3271 3310 3550 3793 4207 4485 4576 4717 4986
Size: 39 240 243 414 278 91 141 269
Cuts at: 4986 5802 6106 6313 6439 3271
Size: 816 304 207 126 7084
Fragments arranged by size:
7084 816 414 304 278 269 243 240
207 141 126 91 39
EcoRI G'AATT_C
Cuts at: 6182 7535 7561 7939 6182
Size: 1353 26 378 8495
Fragments arranged by size:
8495 1353 378 26
EcoRV GAT'ATC
Cuts at: 2203 2203
Size: 10252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4018 4261 4018
Size: 243 10009
FspI TGC'GCA
Cuts at: 4970 5777 8758 4970
Size: 807 2981 6464
Fragments arranged by size:
6464 2981 807
HaeII r_GCGC'y
Cuts at: 1678 2074 5820 6358 6931 9399 1678
Size: 396 3746 538 573 2468 2531
Fragments arranged by size:
3746 2531 2468 573 538 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2355 2387 3584 3616 4832
Size: 32 135 32 783 32 1197 32 1216
Cuts at: 4832 4864 5183 5215 5609 5641 6629 6661 7103
Size: 32 319 32 394 32 988 32 442
Cuts at: 7103 7135 8893 8925 8967 8999 1373
Size: 32 1758 32 42 32 2626
Fragments arranged by size:
2626 1758 1216 1197 988 783 442 394
319 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2085 5902 7596 7842 669
Size: 988 281 147 3817 1694 246 3079
Fragments arranged by size:
3817 3079 1694 988 281 246 147
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10252
HpaI GTT'AAC
Cuts at: 2085 7842 2085
Size: 5757 4495
KpnI G_GTAC'C
Cuts at: 2701 5209 7577 2701
Size: 2508 2368 5376
Fragments arranged by size:
5376 2508 2368
MfeI C'AATT_G
Cuts at: 2157 7851 2157
Size: 5694 4558
MluI A'CGCG_T
Cuts at: 10128 10128
Size: 10252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2316 3513 3660 3900 4035 5217 6684 6852
Size: 1011 1197 147 240 135 1182 1467 168
Cuts at: 6852 9366 9550 1305
Size: 2514 184 2007
Fragments arranged by size:
2514 2007 1467 1197 1182 1011 240 184
168 147 135
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2391 2456 3903 10
Size: 54 1425 343 559 65 1447 6359
Fragments arranged by size:
6359 1447 1425 559 343 65 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3228 4093 4310 4553 5432 6627
Size: 648 843 1219 865 217 243 879 1195
Cuts at: 6627 7133 7197 8210 8569 8728 518
Size: 506 64 1013 359 159 2042
Fragments arranged by size:
2042 1219 1195 1013 879 865 843 648
506 359 243 217 159 64
MspA1I CmG'CkG
Cuts at: 1606 2078 2179 3972 4079 4648 4792 4798 5506
Size: 472 101 1793 107 569 144 6 708
Cuts at: 5506 5827 6046 6388 6699 7668 8345 9493 9738
Size: 321 219 342 311 969 677 1148 245
Cuts at: 9738 1606
Size: 2120
Fragments arranged by size:
2120 1793 1148 969 708 677 569 472
342 321 311 245 219 144 107 101
6
NaeI GCC'GGC
Cuts at: 5839 5839
Size: 10252
NcoI C'CATG_G
Cuts at: 513 1065 4872 5427 7215 7497 513
Size: 552 3807 555 1788 282 3268
Fragments arranged by size:
3807 3268 1788 555 552 282
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10252
NgoMIV G'CCGG_C
Cuts at: 5837 5837
Size: 10252
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10252
NotI GC'GGCC_GC
Cuts at: 7699 7699
Size: 10252
NspI r_CATG'y
Cuts at: 1132 1229 3413 3634 4480 5900 6415 6529 7138
Size: 97 2184 221 846 1420 515 114 609
Cuts at: 7138 7459 7610 9155 1132
Size: 321 151 1545 2229
Fragments arranged by size:
2229 2184 1545 1420 846 609 515 321
221 151 114 97
PasI CC'CwG_GG
Cuts at: 1348 3815 1348
Size: 2467 7785
PciI A'CATG_T
Cuts at: 6525 7455 9151 6525
Size: 930 1696 7626
Fragments arranged by size:
7626 1696 930
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 2398 5398 6061 6334 7024 1108
Size: 1252 38 3000 663 273 690 4336
Fragments arranged by size:
4336 3000 1252 690 663 273 38
PfoI T'CCnGG_A
Cuts at: 2283 3312 4188 2283
Size: 1029 876 8347
Fragments arranged by size:
8347 1029 876
PmeI GTTT'AAAC
Cuts at: 7556 7556
Size: 10252
PmlI CAC'GTG
Cuts at: 4615 4615
Size: 10252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8321 8353 9834 9866 8321
Size: 32 1481 32 8707
Fragments arranged by size:
8707 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3310 3550 3793 4207 4576 5802 6313 3310
Size: 240 243 414 369 1226 511 7249
Fragments arranged by size:
7249 1226 511 414 369 243 240
PshAI GACnn'nnGTC
Cuts at: 1613 3167 1613
Size: 1554 8698
PsiI TTA'TAA
Cuts at: 7822 7822
Size: 10252
PspOMI G'GGCC_C
Cuts at: 4485 4986 4485
Size: 501 9751
PspXI vC'TCGA_Gb
Cuts at: 5768 6101 5768
Size: 333 9919
PstI C_TGCA'G
Cuts at: 830 2257 3189 4181 4344 4389 4797 4950 4983
Size: 1427 932 992 163 45 408 153 33
Cuts at: 4983 5457 6051 7047 7604 830
Size: 474 594 996 557 3478
Fragments arranged by size:
3478 1427 996 992 932 594 557 474
408 163 153 45 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10252
PvuII CAG'CTG
Cuts at: 2078 2179 4079 4792 5506 6046 2078
Size: 101 1900 713 714 540 6284
Fragments arranged by size:
6284 1900 714 713 540 101
SacI G_AGCT'C
Cuts at: 2022 4152 5769 6366 2022
Size: 2130 1617 597 5908
Fragments arranged by size:
5908 2130 1617 597
SalI G'TCGA_C
Cuts at: 1936 7594 1936
Size: 5658 4594
SapI GCTCTTCn'nnn_
Cuts at: 3720 7067 3720
Size: 3347 6905
SbfI CC_TGCA'GG
Cuts at: 7047 7604 7047
Size: 557 9695
ScaI AGT'ACT
Cuts at: 8500 10212 8500
Size: 1712 8540
SexAI A'CCwGG_T
Cuts at: 6456 6456
Size: 10252
SfcI C'TryA_G
Cuts at: 826 1045 2023 2117 2253 2383 3049 3185 4177
Size: 219 978 94 136 130 666 136 992
Cuts at: 4177 4340 4385 4793 4946 4979 5453 5725 6047
Size: 163 45 408 153 33 474 272 322
Cuts at: 6047 7043 7600 8735 9416 9607 826
Size: 996 557 1135 681 191 1471
Fragments arranged by size:
1471 1135 996 992 978 681 666 557
474 408 322 272 219 191 163 153
136 136 130 94 45 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 4493 4493
Size: 10252
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10252
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10252
SmaI CCC'GGG
Cuts at: 1872 4544 7579 1872
Size: 2672 3035 4545
Fragments arranged by size:
4545 3035 2672
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3287 4694 5747 5768 6089 6101
Size: 197 926 1344 1407 1053 21 321 12
Cuts at: 6101 6563 6752 7163 8356 9257 9519 820
Size: 462 189 411 1193 901 262 1553
Fragments arranged by size:
1553 1407 1344 1193 1053 926 901 462
411 321 262 197 189 21 12
SnaBI TAC'GTA
Cuts at: 493 8043 493
Size: 7550 2702
SpeI A'CTAG_T
Cuts at: 152 3763 152
Size: 3611 6641
SphI G_CATG'C
Cuts at: 1132 4480 6415 7138 7610 1132
Size: 3348 1935 723 472 3774
Fragments arranged by size:
3774 3348 1935 723 472
SspI AAT'ATT
Cuts at: 5 52 5228 8176 5
Size: 47 5176 2948 2081
Fragments arranged by size:
5176 2948 2081 47
StuI AGG'CCT
Cuts at: 1517 4496 4535 5924 6012 1517
Size: 2979 39 1389 88 5757
Fragments arranged by size:
5757 2979 1389 88 39
StyI C'CwwG_G
Cuts at: 513 1065 2088 3007 3455 4872 5427 6007 7215
Size: 552 1023 919 448 1417 555 580 1208
Cuts at: 7215 7497 7691 513
Size: 282 194 3074
Fragments arranged by size:
3074 1417 1208 1023 919 580 555 552
448 282 194
SwaI ATTT'AAAT
Cuts at: 5976 5976
Size: 10252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7328 8279 8296 8449 8634 9079 325
Size: 1255 5748 951 17 153 185 445 1498
Fragments arranged by size:
5748 1498 1255 951 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2164 2670 6693
Size: 274 80 33 51 1443 187 506 4023
Cuts at: 6693 8498 10210 96
Size: 1805 1712 138
Fragments arranged by size:
4023 1805 1712 1443 506 274 187 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10212 10244 10212
Size: 32 10220
Tth111I GACn'n_nGTC
Cuts at: 1316 3178 1316
Size: 1862 8390
XbaI T'CTAG_A
Cuts at: 7588 7588
Size: 10252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2608 2896 6223 2608
Size: 288 3327 6637
Fragments arranged by size:
6637 3327 288
XhoI C'TCGA_G
Cuts at: 1943 5768 6101 1943
Size: 3825 333 6094
Fragments arranged by size:
6094 3825 333
XmaI C'CCGG_G
Cuts at: 1870 4542 7577 1870
Size: 2672 3035 4545
Fragments arranged by size:
4545 3035 2672
XmnI GAAnn'nnTTC
Cuts at: 2142 6401 8381 2142
Size: 4259 1980 4013
Fragments arranged by size:
4259 4013 1980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6645 7114 8060 276
Size: 53 83 186 936 5111 469 946 2468
Fragments arranged by size:
5111 2468 946 936 469 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BbeI BfrBI BsiWI DraIII FseI FspAI
KasI NarI NruI NsiI PacI PsrI RsrII SacII
SanDI SfoI SrfI