Restriction map for
pFN21AA1115
(Circular) (Six-base) MAPSORT of: pFN21AA1115.seq Check: 93 from: 1 to: 7348
pFN21AA1115 7348 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2172 2172
Size: 7348
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3341 5158 278
Size: 53 83 186 936 1805 1817 2468
Fragments arranged by size:
2468 1817 1805 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4691 1937
Size: 2754 4594
Acc65I G'GTAC_C
Cuts at: 4669 4669
Size: 7348
AclI AA'CG_TT
Cuts at: 1563 5475 5848 1563
Size: 3912 373 3063
Fragments arranged by size:
3912 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2359 2863 3497 4400 5411 6795 7063 1632
Size: 727 504 634 903 1011 1384 268 1917
Fragments arranged by size:
1917 1384 1011 903 727 634 504 268
AflII C'TTAA_G
Cuts at: 820 1017 2758 820
Size: 197 1741 5410
Fragments arranged by size:
5410 1741 197
AflIII A'CryG_T
Cuts at: 1203 2766 6247 7224 1203
Size: 1563 3481 977 1327
Fragments arranged by size:
3481 1563 1327 977
AgeI A'CCGG_T
Cuts at: 6179 6179
Size: 7348
AhdI GACnn_n'nnGTC
Cuts at: 6077 6077
Size: 7348
AleI CACnn'nnGTG
Cuts at: 1166 2519 1166
Size: 1353 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7348
AlwNI CAG_nnn'CTG
Cuts at: 2587 2647 2708 2818 2995 3475 4273 5091 6663
Size: 60 61 110 177 480 798 818 1572
Cuts at: 6663 2587
Size: 3272
Fragments arranged by size:
3272 1572 818 798 480 177 110 61
60
ApaI G_GGCC'C
Cuts at: 2923 3450 3531 3967 4239 4507 2923
Size: 527 81 436 272 268 5764
Fragments arranged by size:
5764 527 436 272 268 81
ApaLI G'TGCA_C
Cuts at: 5405 6561 5405
Size: 1156 6192
ApoI r'AATT_y
Cuts at: 1493 4657 4883 5035 7144 1493
Size: 3164 226 152 2109 1697
Fragments arranged by size:
3164 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5902 160
Size: 872 4870 1606
Fragments arranged by size:
4870 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7348
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 4185 4673 1870
Size: 73 135 2107 488 4545
Fragments arranged by size:
4545 2107 488 135 73
BamHI G'GATC_C
Cuts at: 2808 3330 4434 4678 5045 2808
Size: 522 1104 244 367 5111
Fragments arranged by size:
5111 1104 522 367 244
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2294 2477 2948 3419 3932
Size: 325 217 627 507 183 471 471 513
Cuts at: 3932 4669 6124 618
Size: 737 1455 1842
Fragments arranged by size:
1842 1455 737 627 513 507 471 471
325 217 183
BanII G_rGCy'C
Cuts at: 1391 2838 2907 2923 3135 3450 3531 3908 3967
Size: 1447 69 16 212 315 81 377 59
Cuts at: 3967 4239 4377 4507 4544 4551 4667 1391
Size: 272 138 130 37 7 116 4072
Fragments arranged by size:
4072 1447 377 315 272 212 138 130
116 81 69 59 37 16 7
BbeI G_GCGC'C
Cuts at: 2952 3936 2952
Size: 984 6364
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2103 3178 4056 928
Size: 971 204 1075 878 4220
Fragments arranged by size:
4220 1075 971 878 204
BbvCI CC'TCA_GC
Cuts at: 2532 2537 4119 4333 4378 2532
Size: 5 1582 214 45 5502
Fragments arranged by size:
5502 1582 214 45 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2752 2786 5539 5573 1903
Size: 34 815 34 2753 34 3678
Fragments arranged by size:
3678 2753 815 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5240 6450 5240
Size: 1210 6138
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7348
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2842 2918 3962
Size: 107 122 71 631 605 1170 76 1044
Cuts at: 3962 4502 4555 5959 136
Size: 540 53 1404 1525
Fragments arranged by size:
1525 1404 1170 1044 631 605 540 122
107 76 71 53
BglII A'GATC_T
Cuts at: 7343 7343
Size: 7348
BlpI GC'TnA_GC
Cuts at: 2975 3236 4765 2975
Size: 261 1529 5558
Fragments arranged by size:
5558 1529 261
Bme1580I G_kGCm'C
Cuts at: 1790 2274 2923 3100 3153 3424 3450 3531 3967
Size: 484 649 177 53 271 26 81 436
Cuts at: 3967 4239 4251 4507 4579 5409 6565 1790
Size: 272 12 256 72 830 1156 2573
Fragments arranged by size:
2573 1156 830 649 484 436 272 271
256 177 81 72 53 26 12
BmgBI CAC'GTC
Cuts at: 1318 2101 3148 1318
Size: 783 1047 5518
Fragments arranged by size:
5518 1047 783
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2232 2635 4151
Size: 416 53 83 399 24 815 403 1516
Cuts at: 4151 4224 5079 6037 442
Size: 73 855 958 1753
Fragments arranged by size:
1753 1516 958 855 815 416 403 399
83 73 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2750 2852 2921 3020 3035 3577 4110
Size: 836 675 102 69 99 15 542 533
Cuts at: 4110 4206 6008 1239
Size: 96 1802 2579
Fragments arranged by size:
2579 1802 836 675 542 533 102 99
96 69 15
Bpu10I CC'TnA_GC
Cuts at: 2069 2532 2537 2666 3101 3907 4119 4307 4333
Size: 463 5 129 435 806 212 188 26
Cuts at: 4333 4378 2069
Size: 45 5039
Fragments arranged by size:
5039 806 463 435 212 188 129 45
26 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3602 3851 5473 6338 6636 3602
Size: 249 1622 865 298 4314
Fragments arranged by size:
4314 1622 865 298 249
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3533 4317 4467 6011 882
Size: 617 2034 784 150 1544 2219
Fragments arranged by size:
2219 2034 1544 784 617 150
BsaAI yAC'GTr
Cuts at: 493 1204 3076 5139 493
Size: 711 1872 2063 2702
Fragments arranged by size:
2702 2063 1872 711
BsaBI GATnn'nnATC
Cuts at: 2619 7342 2619
Size: 4723 2625
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2949 3272 3338 3933
Size: 53 83 186 936 1416 323 66 595
Cuts at: 3933 4296 5155 5537 6172 275
Size: 363 859 382 635 1451
Fragments arranged by size:
1451 1416 936 859 635 595 382 363
323 186 83 66 53
BsaWI w'CCGG_w
Cuts at: 3990 5781 6179 6453 6600 7011 3990
Size: 1791 398 274 147 411 4327
Fragments arranged by size:
4327 1791 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3572 3602 4204 4234 4579
Size: 30 165 30 2169 30 602 30 345
Cuts at: 4579 4609 1178
Size: 30 3917
Fragments arranged by size:
3917 2169 602 345 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2582 3362 3580 3653 3740 3758 3896
Size: 1042 354 780 218 73 87 18 138
Cuts at: 3896 4043 4052 4055 4058 4070 4444 4584 1186
Size: 147 9 3 3 12 374 140 3950
Fragments arranged by size:
3950 1042 780 374 354 218 147 140
138 87 73 18 12 9 3 3
BseYI C'CCAG_C
Cuts at: 3488 4106 4133 4448 4492 6551 7047 3488
Size: 618 27 315 44 2059 496 3789
Fragments arranged by size:
3789 2059 618 496 315 44 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2009 3056 3449 4092 4193 1163
Size: 573 273 1047 393 643 101 4318
Fragments arranged by size:
4318 1047 643 573 393 273 101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3358 3613 4798 5559 6587 1540
Size: 275 186 1357 255 1185 761 1028 2301
Fragments arranged by size:
2301 1357 1185 1028 761 275 255 186
BsiHKAI G_wGCw'C
Cuts at: 2342 2907 3135 3230 5409 5494 6565 2342
Size: 565 228 95 2179 85 1071 3125
Fragments arranged by size:
3125 2179 1071 565 228 95 85
BsmI GAATG_Cn'
Cuts at: 4859 4952 4859
Size: 93 7255
BsmBI CGTCTCn'nnnn_
Cuts at: 2735 3263 2735
Size: 528 6820
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 2342 2838 2907 2923 3100 3135
Size: 399 484 68 496 69 16 177 35
Cuts at: 3135 3153 3230 3424 3450 3531 3908 3967 4239
Size: 18 77 194 26 81 377 59 272
Cuts at: 4239 4251 4377 4507 4544 4551 4579 4667 5409
Size: 12 126 130 37 7 28 88 742
Cuts at: 5409 5494 6565 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 496 484 399 377 272
194 177 130 126 88 85 81 77
69 68 59 37 35 28 26 18
16 12 7
BspEI T'CCGG_A
Cuts at: 3990 7011 3990
Size: 3021 4327
BspHI T'CATG_A
Cuts at: 5237 6967 5237
Size: 1730 5618
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2031 2172 2466 4703 844
Size: 1187 141 294 2237 3489
Fragments arranged by size:
3489 2237 1187 294 141
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 2218 2527 3328 5235 1394
Size: 429 236 159 309 801 1907 3507
Fragments arranged by size:
3507 1907 801 429 309 236 159
BsrDI GCAATG_nn'
Cuts at: 65 1248 2178 5843 6017 65
Size: 1183 930 3665 174 1396
Fragments arranged by size:
3665 1396 1183 930 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2316 2342 3416 3612 5992 6179 1700
Size: 110 506 26 1074 196 2380 187 2869
Fragments arranged by size:
2869 2380 1074 506 196 187 110 26
BsrGI T'GTAC_A
Cuts at: 96 3361 96
Size: 3265 4083
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7348
BssSI C'ACGA_G
Cuts at: 2420 3128 5408 6420 2420
Size: 708 2280 1012 3348
Fragments arranged by size:
3348 2280 1012 708
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7348
BstBI TT'CG_AA
Cuts at: 6152 6152
Size: 7348
BstEII G'GTnAC_C
Cuts at: 1184 2185 3398 1184
Size: 1001 1213 5134
Fragments arranged by size:
5134 1213 1001
BstXI CCAn_nnnn'nTGG
Cuts at: 3983 3983
Size: 7348
BstYI r'GATC_y
Cuts at: 1972 2598 2808 3330 4434 4678 5045 5430 5447
Size: 626 210 522 1104 244 367 385 17
Cuts at: 5447 6899 6985 6997 7343 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1104 626 522 385 367 346
244 210 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3636 4341 3636
Size: 705 6643
BtgI C'CryG_G
Cuts at: 513 1065 2969 4078 4429 513
Size: 552 1904 1109 351 3432
Fragments arranged by size:
3432 1904 1109 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6609
Fragments arranged by size:
6609 683 56
BtsI GCAGTG_nn'
Cuts at: 2357 2890 3317 3512 4864 5658 5678 2357
Size: 533 427 195 1352 794 20 4027
Fragments arranged by size:
4027 1352 794 533 427 195 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7348
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7313
DraI TTT'AAA
Cuts at: 4652 4652
Size: 7348
DraIII CAC_nnn'GTG
Cuts at: 3986 3986
Size: 7348
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6355 809
Size: 507 5039 1802
Fragments arranged by size:
5039 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 2484 2721
Size: 54 1425 50 275 18 534 120 237
Cuts at: 2721 3090 3414 3954 4275 4795 5684 7313 8
Size: 369 324 540 321 520 889 1629 43
Fragments arranged by size:
1629 1425 889 540 534 520 369 324
321 275 237 120 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4795 1537
Size: 275 2983 4090
Fragments arranged by size:
4090 2983 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3176 3873 3888 4020 4197 4532 5278 1353
Size: 1823 697 15 132 177 335 746 3423
Fragments arranged by size:
3423 1823 746 697 335 177 132 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2651 3039 3551 5935 6309 6455
Size: 1064 138 1062 388 512 2384 374 146
Cuts at: 6455 387
Size: 1280
Fragments arranged by size:
2384 1280 1064 1062 512 388 374 146
138
EcoICRI GAG'CTC
Cuts at: 2905 3133 2905
Size: 228 7120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2359 2750 2852 2863 2921 3020
Size: 393 443 284 391 102 11 58 99
Cuts at: 3020 3035 3497 3577 4110 4206 4400 5411 6008
Size: 15 462 80 533 96 194 1011 597
Cuts at: 6008 6795 7063 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1011 787 597 533 462 443 393
391 284 268 194 102 99 96 80
58 15 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3384 3384
Size: 7348
EcoO109I rG'GnC_Cy
Cuts at: 2065 2919 2920 3377 3446 3527 3528 3963 4130
Size: 854 1 457 69 81 1 435 167
Cuts at: 4130 4235 4236 4255 4318 4411 4503 4582 4625
Size: 105 1 19 63 93 92 79 43
Cuts at: 4625 2065
Size: 4788
Fragments arranged by size:
4788 854 457 435 167 105 93 92
81 79 69 63 43 19 1 1
1
EcoRI G'AATT_C
Cuts at: 4657 5035 4657
Size: 378 6970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 7348
FspI TGC'GCA
Cuts at: 5854 5854
Size: 7348
HaeII r_GCGC'y
Cuts at: 1678 2952 3108 3936 6495 1678
Size: 1274 156 828 2559 2531
Fragments arranged by size:
2559 2531 1274 828 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5989 6021 6063 6095 1373
Size: 32 135 32 4417 32 42 32 2626
Fragments arranged by size:
4417 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2125 4692 4938 669
Size: 988 281 187 2567 246 3079
Fragments arranged by size:
3079 2567 988 281 246 187
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7348
HpaI GTT'AAC
Cuts at: 4938 4938
Size: 7348
KasI G'GCGC_C
Cuts at: 2948 3932 2948
Size: 984 6364
KpnI G_GTAC'C
Cuts at: 4673 4673
Size: 7348
MfeI C'AATT_G
Cuts at: 2554 4947 2554
Size: 2393 4955
MluI A'CGCG_T
Cuts at: 7224 7224
Size: 7348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 6462 6646 1305
Size: 1350 3807 184 2007
Fragments arranged by size:
3807 2007 1350 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2366 2723 3092 3956 4277
Size: 54 1425 343 534 357 369 864 321
Cuts at: 4277 10
Size: 3081
Fragments arranged by size:
3081 1425 864 534 369 357 343 321
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2519 5306 5665 5824 518
Size: 648 1353 2787 359 159 2042
Fragments arranged by size:
2787 2042 1353 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2536 2584 2899 2971 2979 3463 3472 3952
Size: 930 48 315 72 8 484 9 480
Cuts at: 3952 4137 4764 5441 6589 6834 1606
Size: 185 627 677 1148 245 2120
Fragments arranged by size:
2120 1148 930 677 627 484 480 315
245 185 72 48 9 8
NarI GG'CG_CC
Cuts at: 2949 3933 2949
Size: 984 6364
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6796
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7348
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7348
NotI GC'GGCC_GC
Cuts at: 4795 4795
Size: 7348
NspI r_CATG'y
Cuts at: 1132 1229 2770 3043 3206 4706 6251 1132
Size: 97 1541 273 163 1500 1545 2229
Fragments arranged by size:
2229 1545 1541 1500 273 163 97
PasI CC'CwG_GG
Cuts at: 1348 2290 3901 4252 1348
Size: 942 1611 351 4444
Fragments arranged by size:
4444 1611 942 351
PciI A'CATG_T
Cuts at: 2766 6247 2766
Size: 3481 3867
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2719 4396 1108
Size: 902 709 1677 4060
Fragments arranged by size:
4060 1677 902 709
PfoI T'CCnGG_A
Cuts at: 2691 2691
Size: 7348
PmeI GTTT'AAAC
Cuts at: 4652 4652
Size: 7348
PmlI CAC'GTG
Cuts at: 3076 3076
Size: 7348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5417 5449 6930 6962 5417
Size: 32 1481 32 5803
Fragments arranged by size:
5803 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 4318 4411 2065
Size: 2253 93 5002
Fragments arranged by size:
5002 2253 93
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7348
PsiI TTA'TAA
Cuts at: 4918 4918
Size: 7348
PspOMI G'GGCC_C
Cuts at: 2919 3446 3527 3963 4235 4503 2919
Size: 527 81 436 272 268 5764
Fragments arranged by size:
5764 527 436 272 268 81
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2306 2338 2306
Size: 32 7316
PstI C_TGCA'G
Cuts at: 830 2109 2154 2337 2469 3309 3654 4305 4700
Size: 1279 45 183 132 840 345 651 395
Cuts at: 4700 830
Size: 3478
Fragments arranged by size:
3478 1279 840 651 395 345 183 132
45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7348
PvuII CAG'CTG
Cuts at: 2536 2899 2979 3463 3472 3952 4137 2536
Size: 363 80 484 9 480 185 5747
Fragments arranged by size:
5747 484 480 363 185 80 9
SacI G_AGCT'C
Cuts at: 2907 3135 2907
Size: 228 7120
SacII CC_GC'GG
Cuts at: 2972 2972
Size: 7348
SalI G'TCGA_C
Cuts at: 1936 4690 1936
Size: 2754 4594
SapI GCTCTTCn'nnn_
Cuts at: 4197 4532 4197
Size: 335 7013
SbfI CC_TGCA'GG
Cuts at: 4700 4700
Size: 7348
ScaI AGT'ACT
Cuts at: 5596 7308 5596
Size: 1712 5636
SexAI A'CCwGG_T
Cuts at: 3564 3982 3564
Size: 418 6930
SfcI C'TryA_G
Cuts at: 826 1045 2105 2150 2333 2465 3023 3305 3650
Size: 219 1060 45 183 132 558 282 345
Cuts at: 3650 4164 4301 4696 5831 6512 6703 826
Size: 514 137 395 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 681 558 514 395 345
282 219 191 183 137 132 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 2918 3962 2918
Size: 1044 6304
SfoI GGC'GCC
Cuts at: 2950 3934 2950
Size: 984 6364
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7348
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7348
SmaI CCC'GGG
Cuts at: 1872 4675 1872
Size: 2803 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2758 3617 3830 5452 6353 6615
Size: 197 926 815 859 213 1622 901 262
Cuts at: 6615 820
Size: 1553
Fragments arranged by size:
1622 1553 926 901 859 815 262 213
197
SnaBI TAC'GTA
Cuts at: 493 5139 493
Size: 4646 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7348
SphI G_CATG'C
Cuts at: 1132 3043 3206 4706 1132
Size: 1911 163 1500 3774
Fragments arranged by size:
3774 1911 1500 163
SspI AAT'ATT
Cuts at: 5 52 5272 5
Size: 47 5220 2081
Fragments arranged by size:
5220 2081 47
StuI AGG'CCT
Cuts at: 1517 3659 4394 1517
Size: 2142 735 4471
Fragments arranged by size:
4471 2142 735
StyI C'CwwG_G
Cuts at: 513 1065 2914 4395 4551 4787 513
Size: 552 1849 1481 156 236 3074
Fragments arranged by size:
3074 1849 1481 552 236 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2282 3590 5375 5392 5545 5730
Size: 1255 624 78 1308 1785 17 153 185
Cuts at: 5730 6175 325
Size: 445 1498
Fragments arranged by size:
1785 1498 1308 1255 624 445 185 153
78 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3361 5594 7306
Size: 274 80 33 51 1443 1384 2233 1712
Cuts at: 7306 96
Size: 138
Fragments arranged by size:
2233 1712 1443 1384 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3972 4004 4204 4236 4579 4611 7308
Size: 32 368 32 200 32 343 32 2697
Cuts at: 7308 7340 3572
Size: 32 3580
Fragments arranged by size:
3580 2697 368 343 200 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7348
XbaI T'CTAG_A
Cuts at: 4684 4684
Size: 7348
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3160 3238 4134 4270 3160
Size: 78 896 136 6238
Fragments arranged by size:
6238 896 136 78
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7348
XmaI C'CCGG_G
Cuts at: 1870 4673 1870
Size: 2803 4545
XmnI GAAnn'nnTTC
Cuts at: 2873 5477 2873
Size: 2604 4744
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3339 5156 276
Size: 53 83 186 936 1805 1817 2468
Fragments arranged by size:
2468 1817 1805 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI RsrII SanDI SrfI SwaI