Restriction map for
pFN21AA1104
(Circular) (Six-base) MAPSORT of: pFN21AA1104.seq Check: 8592 from: 1 to: 7816
pFN21AA1104 7816 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5626 278
Size: 53 83 186 936 4090 2468
Fragments arranged by size:
4090 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 4821 5159 1937
Size: 1386 1498 338 4594
Fragments arranged by size:
4594 1498 1386 338
Acc65I G'GTAC_C
Cuts at: 5137 5137
Size: 7816
AclI AA'CG_TT
Cuts at: 1563 5943 6316 1563
Size: 4380 373 3063
Fragments arranged by size:
4380 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2651 4207 4896 5879 7263 7531 1632
Size: 1019 1556 689 983 1384 268 1917
Fragments arranged by size:
1917 1556 1384 1019 983 689 268
AflII C'TTAA_G
Cuts at: 820 1017 2753 820
Size: 197 1736 5883
Fragments arranged by size:
5883 1736 197
AflIII A'CryG_T
Cuts at: 1203 2691 2939 6715 7692 1203
Size: 1488 248 3776 977 1327
Fragments arranged by size:
3776 1488 1327 977 248
AgeI A'CCGG_T
Cuts at: 2388 6647 2388
Size: 4259 3557
AhdI GACnn_n'nnGTC
Cuts at: 6545 6545
Size: 7816
AleI CACnn'nnGTG
Cuts at: 1166 2891 1166
Size: 1725 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2781 2909 5001 1184
Size: 1597 128 2092 3999
Fragments arranged by size:
3999 2092 1597 128
AlwNI CAG_nnn'CTG
Cuts at: 2032 2833 3436 5559 7131 2032
Size: 801 603 2123 1572 2717
Fragments arranged by size:
2717 2123 1572 801 603
ApaI G_GGCC'C
Cuts at: 3075 3075
Size: 7816
ApaLI G'TGCA_C
Cuts at: 5873 7029 5873
Size: 1156 6660
ApoI r'AATT_y
Cuts at: 1493 2841 2864 2927 3422 3452 4143 4682 4698
Size: 1348 23 63 495 30 691 539 16
Cuts at: 4698 4841 5125 5351 5503 7612 1493
Size: 143 284 226 152 2109 1697
Fragments arranged by size:
2109 1697 1348 691 539 495 284 226
152 143 63 30 23 16
AseI AT'TA_AT
Cuts at: 160 1032 6370 160
Size: 872 5338 1606
Fragments arranged by size:
5338 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7816
AvaI C'yCGr_G
Cuts at: 1870 1943 2177 3827 5072 5141 1870
Size: 73 234 1650 1245 69 4545
Fragments arranged by size:
4545 1650 1245 234 73 69
AvrII C'CTAG_G
Cuts at: 2921 2921
Size: 7816
BamHI G'GATC_C
Cuts at: 5146 5513 5146
Size: 367 7449
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2893 4158 5137 6592 618
Size: 325 217 627 1106 1265 979 1455 1842
Fragments arranged by size:
1842 1455 1265 1106 979 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2116 3075 3396 4134 5135 1391
Size: 725 959 321 738 1001 4072
Fragments arranged by size:
4072 1001 959 738 725 321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2256 3038 3045 3935 4401 4571 4873
Size: 971 357 782 7 890 466 170 302
Cuts at: 4873 928
Size: 3871
Fragments arranged by size:
3871 971 890 782 466 357 302 170
7
BbvCI CC'TCA_GC
Cuts at: 2042 3836 4362 2042
Size: 1794 526 5496
Fragments arranged by size:
5496 1794 526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6007 6041 1903
Size: 34 4070 34 3678
Fragments arranged by size:
4070 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2451 5708 6918 2451
Size: 3257 1210 3349
Fragments arranged by size:
3349 3257 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7816
BfrBI ATG'CAT
Cuts at: 4611 4611
Size: 7816
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6427 136
Size: 107 122 71 631 605 4755 1525
Fragments arranged by size:
4755 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3612 7811 3612
Size: 4199 3617
BlpI GC'TnA_GC
Cuts at: 2051 3912 5233 2051
Size: 1861 1321 4634
Fragments arranged by size:
4634 1861 1321
Bme1580I G_kGCm'C
Cuts at: 1790 3075 3852 4161 4473 5877 7033 1790
Size: 1285 777 309 312 1404 1156 2573
Fragments arranged by size:
2573 1404 1285 1156 777 312 309
BmgBI CAC'GTC
Cuts at: 1318 3730 1318
Size: 2412 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4834 5046 5547
Size: 416 53 83 399 24 3417 212 501
Cuts at: 5547 6505 442
Size: 958 1753
Fragments arranged by size:
3417 1753 958 501 416 399 212 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7816
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3966 4997 3966
Size: 1031 6785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3765 3793 4784 6476 1239
Size: 2526 28 991 1692 2579
Fragments arranged by size:
2579 2526 1692 991 28
Bpu10I CC'TnA_GC
Cuts at: 2042 3836 4362 2042
Size: 1794 526 5496
Fragments arranged by size:
5496 1794 526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 3554 3642 5941 6806 7104 2342
Size: 1212 88 2299 865 298 3054
Fragments arranged by size:
3054 2299 1212 865 298 88
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2349 2358 2710 3203 4211 6479 882
Size: 617 850 9 352 493 1008 2268 2219
Fragments arranged by size:
2268 2219 1008 850 617 493 352 9
BsaAI yAC'GTr
Cuts at: 493 1204 2694 2805 3958 4304 4600 5607 493
Size: 711 1490 111 1153 346 296 1007 2702
Fragments arranged by size:
2702 1490 1153 1007 711 346 296 111
BsaBI GATnn'nnATC
Cuts at: 7810 7810
Size: 7816
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2585 5623 6005 6640
Size: 53 83 186 936 1052 3038 382 635
Cuts at: 6640 275
Size: 1451
Fragments arranged by size:
3038 1451 1052 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2388 3007 3932 6249 6647 6921 7068 7479 2388
Size: 619 925 2317 398 274 147 411 2725
Fragments arranged by size:
2725 2317 925 619 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7591
Fragments arranged by size:
7591 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 3845 4000 4106 4350 1186
Size: 1285 1374 155 106 244 4652
Fragments arranged by size:
4652 1374 1285 244 155 106
BseYI C'CCAG_C
Cuts at: 2658 3771 3877 4472 7019 7515 2658
Size: 1113 106 595 2547 496 2959
Fragments arranged by size:
2959 2547 1113 595 496 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2294 2380 1163
Size: 573 558 86 6599
Fragments arranged by size:
6599 573 558 86
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4392 4417 4458 5266 6027 7055
Size: 275 186 2391 25 41 808 761 1028
Cuts at: 7055 1540
Size: 2301
Fragments arranged by size:
2391 2301 1028 808 761 275 186 41
25
BsiHKAI G_wGCw'C
Cuts at: 3396 3963 4098 4134 5048 5877 5962 7033 3396
Size: 567 135 36 914 829 85 1071 4179
Fragments arranged by size:
4179 1071 914 829 567 135 85 36
BsiWI C'GTAC_G
Cuts at: 2805 2805
Size: 7816
BsmI GAATG_Cn'
Cuts at: 4357 4613 5327 5420 4357
Size: 256 714 93 6753
Fragments arranged by size:
6753 714 256 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4787 4787
Size: 7816
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 3075 3396 3852 3963 4098 4134
Size: 399 326 959 321 456 111 135 36
Cuts at: 4134 4161 4473 5048 5135 5877 5962 7033 1391
Size: 27 312 575 87 742 85 1071 2174
Fragments arranged by size:
2174 1071 959 742 575 456 399 326
321 312 135 111 87 85 36 27
BspEI T'CCGG_A
Cuts at: 7479 7479
Size: 7816
BspHI T'CATG_A
Cuts at: 2406 5705 7435 2406
Size: 3299 1730 2787
Fragments arranged by size:
3299 2787 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4041 4218 5171 844
Size: 3197 177 953 3489
Fragments arranged by size:
3489 3197 953 177
BsrBI CCG'CTC
Cuts at: 1394 1823 2055 5703 1394
Size: 429 232 3648 3507
Fragments arranged by size:
3648 3507 429 232
BsrDI GCAATG_nn'
Cuts at: 65 1248 2794 6311 6485 65
Size: 1183 1546 3517 174 1396
Fragments arranged by size:
3517 1546 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2388 6460 6647 1700
Size: 110 578 4072 187 2869
Fragments arranged by size:
4072 2869 578 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7816
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7816
BssSI C'ACGA_G
Cuts at: 2846 3154 3536 5876 6888 2846
Size: 308 382 2340 1012 3774
Fragments arranged by size:
3774 2340 1012 382 308
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4000 794
Size: 3206 4610
BstBI TT'CG_AA
Cuts at: 6620 6620
Size: 7816
BstEII G'GTnAC_C
Cuts at: 1184 2714 1184
Size: 1530 6286
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 3787 4808 2293
Size: 1494 1021 5301
Fragments arranged by size:
5301 1494 1021
BstYI r'GATC_y
Cuts at: 1972 2139 2965 3612 5146 5513 5898 5915 7367
Size: 167 826 647 1534 367 385 17 1452
Cuts at: 7367 7453 7465 7811 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1534 1452 826 647 385 367 346
167 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4289 4289
Size: 7816
BtgI C'CryG_G
Cuts at: 513 1065 2143 2292 2889 4504 513
Size: 552 1078 149 597 1615 3825
Fragments arranged by size:
3825 1615 1078 597 552 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2098 487
Size: 683 56 872 6205
Fragments arranged by size:
6205 872 683 56
BtsI GCAGTG_nn'
Cuts at: 2202 5332 6126 6146 2202
Size: 3130 794 20 3872
Fragments arranged by size:
3872 3130 794 20
ClaI AT'CG_AT
Cuts at: 1554 3512 1554
Size: 1958 5858
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2871 2906 529
Size: 35 2307 35 5439
Fragments arranged by size:
5439 2307 35 35
DraI TTT'AAA
Cuts at: 2599 5120 2599
Size: 2521 5295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4801 6823 809
Size: 507 3485 2022 1802
Fragments arranged by size:
3485 2022 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 5010 5058
Size: 54 1425 50 275 18 411 2769 48
Cuts at: 5058 5263 6152 7781 8
Size: 205 889 1629 43
Fragments arranged by size:
2769 1629 1425 889 411 275 205 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5263 1537
Size: 275 3451 4090
Fragments arranged by size:
4090 3451 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3860 4083 4247 5036 5746 1353
Size: 2507 223 164 789 710 3423
Fragments arranged by size:
3423 2507 789 710 223 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2073 6403 6777 6923 387
Size: 1064 138 484 4330 374 146 1280
Fragments arranged by size:
4330 1280 1064 484 374 146 138
EcoICRI GAG'CTC
Cuts at: 3394 4132 3394
Size: 738 7078
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2651 3765 3793 4207 4784 4896 5879
Size: 393 1019 1114 28 414 577 112 983
Cuts at: 5879 6476 7263 7531 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1114 1019 983 787 597 577 414
393 268 112 28
EcoO109I rG'GnC_Cy
Cuts at: 2029 3382 3840 4207 4777 5095 2029
Size: 1353 458 367 570 318 4750
Fragments arranged by size:
4750 1353 570 458 367 318
EcoRI G'AATT_C
Cuts at: 2927 4143 5125 5503 2927
Size: 1216 982 378 5240
Fragments arranged by size:
5240 1216 982 378
EcoRV GAT'ATC
Cuts at: 3751 4276 3751
Size: 525 7291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4333 4418 4588 4889 4333
Size: 85 170 301 7260
Fragments arranged by size:
7260 301 170 85
FspI TGC'GCA
Cuts at: 6322 6322
Size: 7816
HaeII r_GCGC'y
Cuts at: 1678 2014 6963 1678
Size: 336 4949 2531
Fragments arranged by size:
4949 2531 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2124 2156 3301 3333 3575
Size: 32 135 32 552 32 1145 32 242
Cuts at: 3575 3607 4552 4584 6457 6489 6531 6563 1373
Size: 32 945 32 1873 32 42 32 2626
Fragments arranged by size:
2626 1873 1145 945 552 242 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3644 4822 5160 5406 669
Size: 988 281 1706 1178 338 246 3079
Fragments arranged by size:
3079 1706 1178 988 338 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7816
HpaI GTT'AAC
Cuts at: 5406 5406
Size: 7816
KpnI G_GTAC'C
Cuts at: 5141 5141
Size: 7816
MfeI C'AATT_G
Cuts at: 5415 5415
Size: 7816
MluI A'CGCG_T
Cuts at: 7692 7692
Size: 7816
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3118 3705 4397 6930 7114 1305
Size: 1813 587 692 2533 184 2007
Fragments arranged by size:
2533 2007 1813 692 587 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2243 5060 10
Size: 54 1425 343 411 2817 2766
Fragments arranged by size:
2817 2766 1425 411 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 2405 2891 5774 6133 6292 518
Size: 648 1125 114 486 2883 359 159 2042
Fragments arranged by size:
2883 2042 1125 648 486 359 159 114
MspA1I CmG'CkG
Cuts at: 1606 3743 4227 5232 5909 7057 7302 1606
Size: 2137 484 1005 677 1148 245 2120
Fragments arranged by size:
2137 2120 1148 1005 677 484 245
NcoI C'CATG_G
Cuts at: 513 1065 2143 2292 4504 513
Size: 552 1078 149 2212 3825
Fragments arranged by size:
3825 2212 1078 552 149
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7816
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7816
NotI GC'GGCC_GC
Cuts at: 5263 5263
Size: 7816
NsiI A_TGCA'T
Cuts at: 4613 4613
Size: 7816
NspI r_CATG'y
Cuts at: 1132 1229 2066 2943 5174 6719 1132
Size: 97 837 877 2231 1545 2229
Fragments arranged by size:
2231 2229 1545 877 837 97
PasI CC'CwG_GG
Cuts at: 1348 2910 1348
Size: 1562 6254
PciI A'CATG_T
Cuts at: 2939 6715 2939
Size: 3776 4040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7816
PfoI T'CCnGG_A
Cuts at: 2730 5102 2730
Size: 2372 5444
PmeI GTTT'AAAC
Cuts at: 5120 5120
Size: 7816
PmlI CAC'GTG
Cuts at: 3958 3958
Size: 7816
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5885 5917 7398 7430 5885
Size: 32 1481 32 6271
Fragments arranged by size:
6271 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3382 4207 4777 5095 3382
Size: 825 570 318 6103
Fragments arranged by size:
6103 825 570 318
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7816
PsiI TTA'TAA
Cuts at: 5386 5386
Size: 7816
PspOMI G'GGCC_C
Cuts at: 3071 3071
Size: 7816
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3693 3725 3693
Size: 32 7784
PstI C_TGCA'G
Cuts at: 830 2121 2197 3441 3863 4215 5168 830
Size: 1291 76 1244 422 352 953 3478
Fragments arranged by size:
3478 1291 1244 953 422 352 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7816
PvuII CAG'CTG
Cuts at: 3743 3743
Size: 7816
SacI G_AGCT'C
Cuts at: 3396 4134 3396
Size: 738 7078
SalI G'TCGA_C
Cuts at: 1936 4820 5158 1936
Size: 2884 338 4594
Fragments arranged by size:
4594 2884 338
SanDI GG'GwC_CC
Cuts at: 3382 4777 3382
Size: 1395 6421
SapI GCTCTTCn'nnn_
Cuts at: 5036 5036
Size: 7816
SbfI CC_TGCA'GG
Cuts at: 3441 4215 5168 3441
Size: 774 953 6089
Fragments arranged by size:
6089 953 774
ScaI AGT'ACT
Cuts at: 2302 3764 6064 7776 2302
Size: 1462 2300 1712 2342
Fragments arranged by size:
2342 2300 1712 1462
SexAI A'CCwGG_T
Cuts at: 2160 2160
Size: 7816
SfcI C'TryA_G
Cuts at: 826 1045 2117 2193 2961 3437 3859 4211 5164
Size: 219 1072 76 768 476 422 352 953
Cuts at: 5164 6299 6980 7171 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 953 768 681 476 422
352 219 191 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7816
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7816
SmaI CCC'GGG
Cuts at: 1872 5143 1872
Size: 3271 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2321 2753 3569 3657 5920 6821
Size: 197 926 378 432 816 88 2263 901
Cuts at: 6821 7083 820
Size: 262 1553
Fragments arranged by size:
2263 1553 926 901 816 432 378 262
197 88
SnaBI TAC'GTA
Cuts at: 493 5607 493
Size: 5114 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7816
SphI G_CATG'C
Cuts at: 1132 5174 1132
Size: 4042 3774
SspI AAT'ATT
Cuts at: 5 52 4065 5740 5
Size: 47 4013 1675 2081
Fragments arranged by size:
4013 2081 1675 47
StuI AGG'CCT
Cuts at: 1517 3164 4438 1517
Size: 1647 1274 4895
Fragments arranged by size:
4895 1647 1274
StyI C'CwwG_G
Cuts at: 513 1065 2143 2292 2463 2589 2921 3112 3184
Size: 552 1078 149 171 126 332 191 72
Cuts at: 3184 4504 5091 5255 513
Size: 1320 587 164 3074
Fragments arranged by size:
3074 1320 1078 587 552 332 191 171
164 149 126 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2222 2796 4403 4592 5843 5860 6013
Size: 1255 642 574 1607 189 1251 17 153
Cuts at: 6013 6198 6643 325
Size: 185 445 1498
Fragments arranged by size:
1607 1498 1255 1251 642 574 445 189
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2300 3762 6062
Size: 274 80 33 51 1443 323 1462 2300
Cuts at: 6062 7774 96
Size: 1712 138
Fragments arranged by size:
2300 1712 1462 1443 323 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2443 2475 2712 2744 7776 7808 2443
Size: 32 237 32 5032 32 2451
Fragments arranged by size:
5032 2451 237 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3731 1316
Size: 2415 5401
XbaI T'CTAG_A
Cuts at: 3628 5152 3628
Size: 1524 6292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2939 3779 3784 4502 2939
Size: 840 5 718 6253
Fragments arranged by size:
6253 840 718 5
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7816
XmaI C'CCGG_G
Cuts at: 1870 5141 1870
Size: 3271 4545
XmnI GAAnn'nnTTC
Cuts at: 2414 5945 2414
Size: 3531 4285
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5624 276
Size: 53 83 186 936 4090 2468
Fragments arranged by size:
4090 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BstZ17I DraIII
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspXI RsrII SacII SfiI SfoI SrfI SwaI