Restriction map for
pFN21AA1091
(Circular) (Six-base) MAPSORT of: pFN21AA1091.seq Check: 341 from: 1 to: 8566
pFN21AA1091 8566 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4137 4137
Size: 8566
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5843 6376 278
Size: 53 83 186 936 4307 533 2468
Fragments arranged by size:
4307 2468 936 533 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5909 1937
Size: 3972 4594
Acc65I G'GTAC_C
Cuts at: 5887 5887
Size: 8566
AclI AA'CG_TT
Cuts at: 1563 6693 7066 1563
Size: 5130 373 3063
Fragments arranged by size:
5130 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2202 3291 3329 4289 4727 6629 8013 8281
Size: 570 1089 38 960 438 1902 1384 268
Cuts at: 8281 1632
Size: 1917
Fragments arranged by size:
1917 1902 1384 1089 960 570 438 268
38
AfeI AGC'GCT
Cuts at: 2573 4754 2573
Size: 2181 6385
AflII C'TTAA_G
Cuts at: 820 1017 3392 820
Size: 197 2375 5994
Fragments arranged by size:
5994 2375 197
AflIII A'CryG_T
Cuts at: 1203 5742 7465 8442 1203
Size: 4539 1723 977 1327
Fragments arranged by size:
4539 1723 1327 977
AgeI A'CCGG_T
Cuts at: 3114 7397 3114
Size: 4283 4283
AhdI GACnn_n'nnGTC
Cuts at: 2107 7295 2107
Size: 5188 3378
AleI CACnn'nnGTG
Cuts at: 1166 2555 4361 1166
Size: 1389 1806 5371
Fragments arranged by size:
5371 1806 1389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2468 1184
Size: 1284 7282
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5439 5471 5439
Size: 32 8534
AlwNI CAG_nnn'CTG
Cuts at: 2965 5665 6309 7881 2965
Size: 2700 644 1572 3650
Fragments arranged by size:
3650 2700 1572 644
ApaLI G'TGCA_C
Cuts at: 6623 7779 6623
Size: 1156 7410
ApoI r'AATT_y
Cuts at: 1493 2191 3261 5875 6101 6253 8362 1493
Size: 698 1070 2614 226 152 2109 1697
Fragments arranged by size:
2614 2109 1697 1070 698 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7120 160
Size: 872 6088 1606
Fragments arranged by size:
6088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8566
AvaI C'yCGr_G
Cuts at: 1870 1943 2882 3822 5891 1870
Size: 73 939 940 2069 4545
Fragments arranged by size:
4545 2069 940 939 73
BamHI G'GATC_C
Cuts at: 5181 5896 6263 5181
Size: 715 367 7484
Fragments arranged by size:
7484 715 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2153 2755 5887 7342
Size: 325 217 627 248 118 602 3132 1455
Cuts at: 7342 618
Size: 1842
Fragments arranged by size:
3132 1842 1455 627 602 325 248 217
118
BanII G_rGCy'C
Cuts at: 1391 2033 2349 3705 4734 4910 5590 5885 1391
Size: 642 316 1356 1029 176 680 295 4072
Fragments arranged by size:
4072 1356 1029 680 642 316 295 176
BbeI G_GCGC'C
Cuts at: 2039 2039
Size: 8566
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2553 3480 4840 5488 928
Size: 971 654 927 1360 648 4006
Fragments arranged by size:
4006 1360 971 927 654 648
BbvCI CC'TCA_GC
Cuts at: 2703 2703
Size: 8566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5845 5879 6757 6791 1903
Size: 34 3908 34 878 34 3678
Fragments arranged by size:
3908 3678 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6458 7668 6458
Size: 1210 7356
BclI T'GATC_A
Cuts at: 1548 5763 1548
Size: 4215 4351
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2311 5717 7177
Size: 107 122 71 631 605 639 3406 1460
Cuts at: 7177 136
Size: 1525
Fragments arranged by size:
3406 1525 1460 639 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2439 8561 2439
Size: 6122 2444
BlpI GC'TnA_GC
Cuts at: 5983 5983
Size: 8566
Bme1580I G_kGCm'C
Cuts at: 1790 2920 6627 7783 1790
Size: 1130 3707 1156 2573
Fragments arranged by size:
3707 2573 1156 1130
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8566
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2034 3450 4053
Size: 416 53 83 399 24 617 1416 603
Cuts at: 4053 4081 5256 5328 6297 7255 442
Size: 28 1175 72 969 958 1753
Fragments arranged by size:
1753 1416 1175 969 958 617 603 416
399 83 72 53 28 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8566
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 3316 3352 5708 2401
Size: 915 36 2356 5259
Fragments arranged by size:
5259 2356 915 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2945 3146 3752 4435 5195 5855 7226
Size: 875 831 201 606 683 760 660 1371
Cuts at: 7226 1239
Size: 2579
Fragments arranged by size:
2579 1371 875 831 760 683 660 606
201
Bpu10I CC'TnA_GC
Cuts at: 2703 3699 4017 2703
Size: 996 318 7252
Fragments arranged by size:
7252 996 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2744 3120 3706 6691 7556 7854 2744
Size: 376 586 2985 865 298 3456
Fragments arranged by size:
3456 2985 865 586 376 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2078 2514 2815 3728 7229 882
Size: 617 579 436 301 913 3501 2219
Fragments arranged by size:
3501 2219 913 617 579 436 301
BsaAI yAC'GTr
Cuts at: 493 1204 6357 493
Size: 711 5153 2702
Fragments arranged by size:
5153 2702 711
BsaBI GATnn'nnATC
Cuts at: 5470 8560 5470
Size: 3090 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 5840 6373 6755
Size: 53 83 186 936 503 3804 533 382
Cuts at: 6755 7390 275
Size: 635 1451
Fragments arranged by size:
3804 1451 936 635 533 503 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3114 5271 6999 7397 7671 7818 8229 3114
Size: 2157 1728 398 274 147 411 3451
Fragments arranged by size:
3451 2157 1728 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2882 2912 3849 3879 1178
Size: 30 165 30 1479 30 937 30 5865
Fragments arranged by size:
5865 1479 937 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4706 1186
Size: 3520 5046
BseYI C'CCAG_C
Cuts at: 3040 3479 3617 4900 5208 7769 8265 3040
Size: 439 138 1283 308 2561 496 3341
Fragments arranged by size:
3341 2561 1283 496 439 308 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5721 1163
Size: 573 3985 4008
Fragments arranged by size:
4008 3985 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6016 6777 7805 1540
Size: 275 186 4015 761 1028 2301
Fragments arranged by size:
4015 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4734 6627 6712 7783 4734
Size: 1893 85 1071 5517
Fragments arranged by size:
5517 1893 1071 85
BsmI GAATG_Cn'
Cuts at: 2298 2318 3094 3376 4081 4280 6077 6170 2298
Size: 20 776 282 705 199 1797 93 4694
Fragments arranged by size:
4694 1797 776 705 282 199 93 20
BsmBI CGTCTCn'nnnn_
Cuts at: 2617 2975 5831 2617
Size: 358 2856 5352
Fragments arranged by size:
5352 2856 358
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2349 2920 3705 4734 4910 5590
Size: 399 243 316 571 785 1029 176 680
Cuts at: 5590 5885 6627 6712 7783 1391
Size: 295 742 85 1071 2174
Fragments arranged by size:
2174 1071 1029 785 742 680 571 399
316 295 243 176 85
BspEI T'CCGG_A
Cuts at: 5271 8229 5271
Size: 2958 5608
BspHI T'CATG_A
Cuts at: 2649 3778 6455 8185 2649
Size: 1129 2677 1730 3030
Fragments arranged by size:
3030 2677 1730 1129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3717 4137 5921 844
Size: 2873 420 1784 3489
Fragments arranged by size:
3489 2873 1784 420
BsrBI CCG'CTC
Cuts at: 1394 1823 2761 5179 6453 1394
Size: 429 938 2418 1274 3507
Fragments arranged by size:
3507 2418 1274 938 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2457 3283 3357 5810 7061 7235 65
Size: 1183 1209 826 74 2453 1251 174 1396
Fragments arranged by size:
2453 1396 1251 1209 1183 826 174 74
BsrFI r'CCGG_y
Cuts at: 1700 1810 2775 3114 4485 5139 7210 7397 1700
Size: 110 965 339 1371 654 2071 187 2869
Fragments arranged by size:
2869 2071 1371 965 654 339 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8566
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8566
BssSI C'ACGA_G
Cuts at: 6626 7638 6626
Size: 1012 7554
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5812 794
Size: 5018 3548
BstBI TT'CG_AA
Cuts at: 7370 7370
Size: 8566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8566
BstXI CCAn_nnnn'nTGG
Cuts at: 3106 3217 4180 5077 3106
Size: 111 963 897 6595
Fragments arranged by size:
6595 963 897 111
BstYI r'GATC_y
Cuts at: 1972 2439 3493 3549 4410 4587 4932 5181 5325
Size: 467 1054 56 861 177 345 249 144
Cuts at: 5325 5896 6263 6648 6665 8117 8203 8215 8561
Size: 571 367 385 17 1452 86 12 346
Cuts at: 8561 1972
Size: 1977
Fragments arranged by size:
1977 1452 1054 861 571 467 385 367
346 345 249 177 144 86 56 17
12
Bsu36I CC'TnA_GG
Cuts at: 4522 4583 5214 5565 4522
Size: 61 631 351 7523
Fragments arranged by size:
7523 631 351 61
BtgI C'CryG_G
Cuts at: 513 1065 4677 4928 513
Size: 552 3612 251 4151
Fragments arranged by size:
4151 3612 552 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7827
Fragments arranged by size:
7827 683 56
BtsI GCAGTG_nn'
Cuts at: 2452 2714 3295 4917 5610 5733 6082 6876 6896
Size: 262 581 1622 693 123 349 794 20
Cuts at: 6896 2452
Size: 4122
Fragments arranged by size:
4122 1622 794 693 581 349 262 123
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8531
DraI TTT'AAA
Cuts at: 5470 5870 5470
Size: 400 8166
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2060 2858 7573 809
Size: 507 744 798 4715 1802
Fragments arranged by size:
4715 1802 798 744 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2773 5044 5137
Size: 54 1425 50 275 18 943 2271 93
Cuts at: 5137 6013 6902 8531 8
Size: 876 889 1629 43
Fragments arranged by size:
2271 1629 1425 943 889 876 275 93
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6013 1537
Size: 275 4201 4090
Fragments arranged by size:
4201 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2415 3305 4362 5489 6496 1353
Size: 1062 890 1057 1127 1007 3423
Fragments arranged by size:
3423 1127 1062 1057 1007 890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2037 2993 4111 4184 7153 7527
Size: 1064 138 448 956 1118 73 2969 374
Cuts at: 7527 7673 387
Size: 146 1280
Fragments arranged by size:
2969 1280 1118 1064 956 448 374 146
138 73
EcoICRI GAG'CTC
Cuts at: 4732 4732
Size: 8566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2202 2945 3146 3291 3329 3752
Size: 393 482 88 743 201 145 38 423
Cuts at: 3752 4289 4435 4727 5195 5855 6629 7226 8013
Size: 537 146 292 468 660 774 597 787
Cuts at: 8013 8281 1239
Size: 268 1524
Fragments arranged by size:
1524 787 774 743 660 597 537 482
468 423 393 292 268 201 146 145
88 38
EcoNI CCTnn'n_nnAGG
Cuts at: 4268 4598 5849 4268
Size: 330 1251 6985
Fragments arranged by size:
6985 1251 330
EcoO109I rG'GnC_Cy
Cuts at: 2233 4261 2233
Size: 2028 6538
EcoRI G'AATT_C
Cuts at: 3261 5875 6253 3261
Size: 2614 378 5574
Fragments arranged by size:
5574 2614 378
EcoRV GAT'ATC
Cuts at: 2246 2246
Size: 8566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3296 3296
Size: 8566
FspI TGC'GCA
Cuts at: 7072 7072
Size: 8566
HaeII r_GCGC'y
Cuts at: 1678 2039 2575 4756 7713 1678
Size: 361 536 2181 2957 2531
Fragments arranged by size:
2957 2531 2181 536 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2977 3009 3101
Size: 32 135 32 521 32 852 32 92
Cuts at: 3101 3133 3617 3649 3662 3694 3847 3879 4402
Size: 32 484 32 13 32 153 32 523
Cuts at: 4402 4434 4550 4582 7207 7239 7281 7313 1373
Size: 32 116 32 2625 32 42 32 2626
Fragments arranged by size:
2626 2625 852 523 521 484 153 135
116 92 42 32 32 32 32 32
32 32 32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3829 5910 6156 669
Size: 988 281 1891 2081 246 3079
Fragments arranged by size:
3079 2081 1891 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5024 748
Size: 4276 4290
HpaI GTT'AAC
Cuts at: 6156 6156
Size: 8566
KasI G'GCGC_C
Cuts at: 2035 2035
Size: 8566
KpnI G_GTAC'C
Cuts at: 5891 5891
Size: 8566
MfeI C'AATT_G
Cuts at: 3918 4243 6165 3918
Size: 325 1922 6319
Fragments arranged by size:
6319 1922 325
MluI A'CGCG_T
Cuts at: 8442 8442
Size: 8566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2642 3441 3637 4236 7680 7864 1305
Size: 1337 799 196 599 3444 184 2007
Fragments arranged by size:
3444 2007 1337 799 599 196 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5046 10
Size: 54 1425 343 3214 3530
Fragments arranged by size:
3530 3214 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2555 2639 3024 3099 3288 3581 3783
Size: 648 1389 84 385 75 189 293 202
Cuts at: 3783 4361 5745 6524 6883 7042 518
Size: 578 1384 779 359 159 2042
Fragments arranged by size:
2042 1389 1384 779 648 578 385 359
293 202 189 159 84 75
MspA1I CmG'CkG
Cuts at: 1606 5248 5982 6659 7807 8052 1606
Size: 3642 734 677 1148 245 2120
Fragments arranged by size:
3642 2120 1148 734 677 245
NarI GG'CG_CC
Cuts at: 2036 2036
Size: 8566
NcoI C'CATG_G
Cuts at: 513 1065 4677 4928 513
Size: 552 3612 251 4151
Fragments arranged by size:
4151 3612 552 251
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8566
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8566
NotI GC'GGCC_GC
Cuts at: 6013 6013
Size: 8566
NspI r_CATG'y
Cuts at: 1132 1229 2473 2501 2661 3023 5746 5924 7469
Size: 97 1244 28 160 362 2723 178 1545
Cuts at: 7469 1132
Size: 2229
Fragments arranged by size:
2723 2229 1545 1244 362 178 160 97
28
PasI CC'CwG_GG
Cuts at: 1348 2340 1348
Size: 992 7574
PciI A'CATG_T
Cuts at: 5742 7465 5742
Size: 1723 6843
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2771 4662 4929 5017 1108
Size: 1663 1891 267 88 4657
Fragments arranged by size:
4657 1891 1663 267 88
PfoI T'CCnGG_A
Cuts at: 3228 3552 3729 4164 4401 5328 5511 3228
Size: 324 177 435 237 927 183 6283
Fragments arranged by size:
6283 927 435 324 237 183 177
PmeI GTTT'AAAC
Cuts at: 5870 5870
Size: 8566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6635 6667 8148 8180 6635
Size: 32 1481 32 7021
Fragments arranged by size:
7021 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2233 2233
Size: 8566
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8566
PsiI TTA'TAA
Cuts at: 6136 6136
Size: 8566
PstI C_TGCA'G
Cuts at: 830 2447 2571 2676 4026 5253 5808 5918 830
Size: 1617 124 105 1350 1227 555 110 3478
Fragments arranged by size:
3478 1617 1350 1227 555 124 110 105
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8566
SacI G_AGCT'C
Cuts at: 4734 4734
Size: 8566
SalI G'TCGA_C
Cuts at: 1936 5908 1936
Size: 3972 4594
SapI GCTCTTCn'nnn_
Cuts at: 5489 5489
Size: 8566
SbfI CC_TGCA'GG
Cuts at: 5918 5918
Size: 8566
ScaI AGT'ACT
Cuts at: 6814 8526 6814
Size: 1712 6854
SfcI C'TryA_G
Cuts at: 826 1045 2443 2567 2672 4022 5249 5804 5914
Size: 219 1398 124 105 1350 1227 555 110
Cuts at: 5914 7049 7730 7921 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1398 1350 1227 1135 681 555 219
191 124 110 105
SfoI GGC'GCC
Cuts at: 2037 2037
Size: 8566
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8566
SmaI CCC'GGG
Cuts at: 1872 5893 1872
Size: 4021 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2723 3135 3392 3721 6670 7571
Size: 197 926 780 412 257 329 2949 901
Cuts at: 7571 7833 820
Size: 262 1553
Fragments arranged by size:
2949 1553 926 901 780 412 329 262
257 197
SnaBI TAC'GTA
Cuts at: 493 6357 493
Size: 5864 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8566
SphI G_CATG'C
Cuts at: 1132 5924 1132
Size: 4792 3774
SspI AAT'ATT
Cuts at: 5 52 6490 5
Size: 47 6438 2081
Fragments arranged by size:
6438 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8566
StyI C'CwwG_G
Cuts at: 513 1065 3426 4677 4928 5442 6005 513
Size: 552 2361 1251 251 514 563 3074
Fragments arranged by size:
3074 2361 1251 563 552 514 251
SwaI ATTT'AAAT
Cuts at: 5470 5470
Size: 8566
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2653 3975 4654 4943 5308 6593 6610
Size: 1255 1073 1322 679 289 365 1285 17
Cuts at: 6610 6763 6948 7393 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1322 1285 1255 1073 679 445 365
289 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 3802 4033
Size: 274 80 33 51 1443 147 1678 231
Cuts at: 4033 4158 6812 8524 96
Size: 125 2654 1712 138
Fragments arranged by size:
2654 1712 1678 1443 274 231 147 138
125 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3849 3881 4416 4448 8526 8558 3849
Size: 32 535 32 4078 32 3857
Fragments arranged by size:
4078 3857 535 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8566
XbaI T'CTAG_A
Cuts at: 5902 5902
Size: 8566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4297 5054 5077 4297
Size: 757 23 7786
Fragments arranged by size:
7786 757 23
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8566
XmaI C'CCGG_G
Cuts at: 1870 5891 1870
Size: 4021 4545
XmnI GAAnn'nnTTC
Cuts at: 5369 6695 5369
Size: 1326 7240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5841 6374 276
Size: 53 83 186 936 4307 533 2468
Fragments arranged by size:
4307 2468 936 533 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SrfI