Restriction map for
pFN21AA1082
(Circular) (Six-base) MAPSORT of: pFN21AA1082.seq Check: 9695 from: 1 to: 9988
pFN21AA1082 9988 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3148 7198 3148
Size: 4050 5938
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7798 278
Size: 53 83 186 936 6262 2468
Fragments arranged by size:
6262 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2931 3035 5389 6333 7331 1937
Size: 994 104 2354 944 998 4594
Fragments arranged by size:
4594 2354 998 994 944 104
Acc65I G'GTAC_C
Cuts at: 4767 6960 7309 4767
Size: 2193 349 7446
Fragments arranged by size:
7446 2193 349
AclI AA'CG_TT
Cuts at: 1563 8115 8488 1563
Size: 6552 373 3063
Fragments arranged by size:
6552 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4526 4697 5589 8051 9435 9703 1632
Size: 2894 171 892 2462 1384 268 1917
Fragments arranged by size:
2894 2462 1917 1384 892 268 171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9791
AflIII A'CryG_T
Cuts at: 1203 7129 8887 9864 1203
Size: 5926 1758 977 1327
Fragments arranged by size:
5926 1758 1327 977
AgeI A'CCGG_T
Cuts at: 8819 8819
Size: 9988
AhdI GACnn_n'nnGTC
Cuts at: 5417 8717 5417
Size: 3300 6688
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2057 6870 1184
Size: 873 4813 4302
Fragments arranged by size:
4813 4302 873
AlwNI CAG_nnn'CTG
Cuts at: 3320 3338 6354 7731 9303 3320
Size: 18 3016 1377 1572 4005
Fragments arranged by size:
4005 3016 1572 1377 18
ApaI G_GGCC'C
Cuts at: 2973 4148 4544 6617 2973
Size: 1175 396 2073 6344
Fragments arranged by size:
6344 2073 1175 396
ApaLI G'TGCA_C
Cuts at: 8045 9201 8045
Size: 1156 8832
ApoI r'AATT_y
Cuts at: 1493 2207 3385 3394 4512 6314 7160 7297 7523
Size: 714 1178 9 1118 1802 846 137 226
Cuts at: 7523 7675 9784 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1802 1697 1178 1118 846 714 226
152 137 9
AseI AT'TA_AT
Cuts at: 160 1032 8542 160
Size: 872 7510 1606
Fragments arranged by size:
7510 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9988
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 3376 3891 4028 5346 7313 1870
Size: 73 193 1240 515 137 1318 1967 4545
Fragments arranged by size:
4545 1967 1318 1240 515 193 137 73
AvrII C'CTAG_G
Cuts at: 7033 7033
Size: 9988
BamHI G'GATC_C
Cuts at: 7318 7685 7318
Size: 367 9621
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 2299 4015 4726 4767
Size: 325 217 627 392 120 1716 711 41
Cuts at: 4767 5506 6960 7250 7309 8764 618
Size: 739 1454 290 59 1455 1842
Fragments arranged by size:
1842 1716 1455 1454 739 711 627 392
325 290 217 120 59 41
BanII G_rGCy'C
Cuts at: 1391 2973 3701 4127 4148 4544 4695 6288 6617
Size: 1582 728 426 21 396 151 1593 329
Cuts at: 6617 7066 7307 1391
Size: 449 241 4072
Fragments arranged by size:
4072 1593 1582 728 449 426 396 329
241 151 21
BbeI G_GCGC'C
Cuts at: 2303 2303
Size: 9988
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5950 7116 928
Size: 971 4051 1166 3800
Fragments arranged by size:
4051 3800 1166 971
BbvCI CC'TCA_GC
Cuts at: 2071 2256 5971 2071
Size: 185 3715 6088
Fragments arranged by size:
6088 3715 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4727 4761 6767 6801 8179 8213 1903
Size: 34 2790 34 2006 34 1378 34 3678
Fragments arranged by size:
3678 2790 2006 1378 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7880 9090 7880
Size: 1210 8778
BclI T'GATC_A
Cuts at: 1548 2547 6931 1548
Size: 999 4384 4605
Fragments arranged by size:
4605 4384 999
BfrBI ATG'CAT
Cuts at: 3591 4716 3591
Size: 1125 8863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 8599 136
Size: 107 122 71 631 605 475 6452 1525
Fragments arranged by size:
6452 1525 631 605 475 122 107 71
BglII A'GATC_T
Cuts at: 4951 5564 6213 6514 9983 4951
Size: 613 649 301 3469 4956
Fragments arranged by size:
4956 3469 649 613 301
BlpI GC'TnA_GC
Cuts at: 4126 4563 7405 4126
Size: 437 2842 6709
Fragments arranged by size:
6709 2842 437
Bme1580I G_kGCm'C
Cuts at: 1790 2184 2973 4148 4544 6617 8049 9205 1790
Size: 394 789 1175 396 2073 1432 1156 2573
Fragments arranged by size:
2573 2073 1432 1175 1156 789 396 394
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9988
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2309 2399 5140
Size: 416 53 83 399 24 892 90 2741
Cuts at: 5140 7719 8677 442
Size: 2579 958 1753
Fragments arranged by size:
2741 2579 1753 958 892 416 399 90
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9988
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 4054 3446
Size: 608 9380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 3795 4162 4423 4707 4781 5505 7080
Size: 1070 1486 367 261 284 74 724 1575
Cuts at: 7080 7107 8648 1239
Size: 27 1541 2579
Fragments arranged by size:
2579 1575 1541 1486 1070 724 367 284
261 74 27
Bpu10I CC'TnA_GC
Cuts at: 2071 2256 3695 3700 5180 5971 2071
Size: 185 1439 5 1480 791 6088
Fragments arranged by size:
6088 1480 1439 791 185 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3707 6260 6443 6749 7241 8113 8978 9276 3707
Size: 2553 183 306 492 872 865 298 4419
Fragments arranged by size:
4419 2553 872 865 492 306 298 183
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2292 4952 5404 5806 5880 6315 8651
Size: 617 793 2660 452 402 74 435 2336
Cuts at: 8651 882
Size: 2219
Fragments arranged by size:
2660 2336 2219 793 617 452 435 402
74
BsaAI yAC'GTr
Cuts at: 493 1204 7779 493
Size: 711 6575 2702
Fragments arranged by size:
6575 2702 711
BsaBI GATnn'nnATC
Cuts at: 6731 9982 6731
Size: 3251 6737
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2300 7795 8177 8812
Size: 53 83 186 936 767 5495 382 635
Cuts at: 8812 275
Size: 1451
Fragments arranged by size:
5495 1451 936 767 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3774 4770 4830 8421 8819 9093 9240 9651 3774
Size: 996 60 3591 398 274 147 411 4111
Fragments arranged by size:
4111 3591 996 411 398 274 147 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2356 2386 2854 2884 3793
Size: 30 165 30 953 30 468 30 909
Cuts at: 3793 3813 3823 3843 4421 4451 5319 5349 1178
Size: 20 10 20 578 30 868 30 5817
Fragments arranged by size:
5817 953 909 868 578 468 165 30
30 30 30 30 30 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2875 3973 4030 4087 4219 4259 4277 4493
Size: 1689 1098 57 57 132 40 18 216
Cuts at: 4493 5511 5650 7010 1186
Size: 1018 139 1360 4164
Fragments arranged by size:
4164 1689 1360 1098 1018 216 139 132
57 57 40 18
BseYI C'CCAG_C
Cuts at: 4019 4537 4573 4887 5839 6191 7014 9191 9687
Size: 518 36 314 952 352 823 2177 496
Cuts at: 9687 4019
Size: 4320
Fragments arranged by size:
4320 2177 952 823 518 496 352 314
36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2505 3131 3522 4721 5654 7046 1163
Size: 573 769 626 391 1199 933 1392 4105
Fragments arranged by size:
4105 1392 1199 933 769 626 573 391
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3368 7438 8199 9227 1540
Size: 275 186 1367 4070 761 1028 2301
Fragments arranged by size:
4070 2301 1367 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3427 3701 4524 5745 6288 6453 6748 8049 8134
Size: 274 823 1221 543 165 295 1301 85
Cuts at: 8134 9205 3427
Size: 1071 4210
Fragments arranged by size:
4210 1301 1221 1071 823 543 295 274
165 85
BsmI GAATG_Cn'
Cuts at: 2331 2992 4757 7499 7592 2331
Size: 661 1765 2742 93 4727
Fragments arranged by size:
4727 2742 1765 661 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2973 3427 3701 4127 4148 4524
Size: 399 394 789 454 274 426 21 376
Cuts at: 4524 4544 4695 5745 6288 6453 6617 6748 7066
Size: 20 151 1050 543 165 164 131 318
Cuts at: 7066 7307 8049 8134 9205 1391
Size: 241 742 85 1071 2174
Fragments arranged by size:
2174 1071 1050 789 742 543 454 426
399 394 376 318 274 241 165 164
151 131 85 21 20
BspEI T'CCGG_A
Cuts at: 4830 9651 4830
Size: 4821 5167
BspHI T'CATG_A
Cuts at: 3856 6748 6814 6934 6946 7192 7264 7877 9607
Size: 2892 66 120 12 246 72 613 1730
Cuts at: 9607 3856
Size: 4237
Fragments arranged by size:
4237 2892 1730 613 246 120 72 66
12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3148 5422 7063 7198 7343 844
Size: 2304 2274 1641 135 145 3489
Fragments arranged by size:
3489 2304 2274 1641 145 135
BsrBI CCG'CTC
Cuts at: 1394 1823 2109 2172 4176 4765 7875 1394
Size: 429 286 63 2004 589 3110 3507
Fragments arranged by size:
3507 3110 2004 589 429 286 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 2343 6021 8483 8657 65
Size: 1183 1095 3678 2462 174 1396
Fragments arranged by size:
3678 2462 1396 1183 1095 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2150 3363 4608 5175 5190 5503 8632
Size: 110 340 1213 1245 567 15 313 3129
Cuts at: 8632 8819 1700
Size: 187 2869
Fragments arranged by size:
3129 2869 1245 1213 567 340 313 187
110 15
BsrGI T'GTAC_A
Cuts at: 96 6624 96
Size: 6528 3460
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9988
BssSI C'ACGA_G
Cuts at: 8048 9060 8048
Size: 1012 8976
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9988
BstBI TT'CG_AA
Cuts at: 8792 8792
Size: 9988
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9988
BstXI CCAn_nnnn'nTGG
Cuts at: 3482 5035 6317 3482
Size: 1553 1282 7153
Fragments arranged by size:
7153 1553 1282
BstYI r'GATC_y
Cuts at: 1972 2113 2731 3309 3719 4951 5564 6133 6213
Size: 141 618 578 410 1232 613 569 80
Cuts at: 6213 6514 6876 7318 7685 8070 8087 9539 9625
Size: 301 362 442 367 385 17 1452 86
Cuts at: 9625 9637 9983 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1232 618 613 578 569 442
410 385 367 362 346 301 141 86
80 17 12
Bsu36I CC'TnA_GG
Cuts at: 5732 5732
Size: 9988
BtgI C'CryG_G
Cuts at: 513 1065 2004 2091 3821 4161 513
Size: 552 939 87 1730 340 6340
Fragments arranged by size:
6340 1730 939 552 340 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2961 3426 487
Size: 683 56 1735 465 7049
Fragments arranged by size:
7049 1735 683 465 56
BtsI GCAGTG_nn'
Cuts at: 2061 4564 5206 5790 5847 7239 7504 8298 8318
Size: 2503 642 584 57 1392 265 794 20
Cuts at: 8318 2061
Size: 3731
Fragments arranged by size:
3731 2503 1392 794 642 584 265 57
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9988
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5117 5152 529
Size: 35 4553 35 5365
Fragments arranged by size:
5365 4553 35 35
DraI TTT'AAA
Cuts at: 7292 7292
Size: 9988
DraIII CAC_nnn'GTG
Cuts at: 5201 5201
Size: 9988
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8995 809
Size: 507 7679 1802
Fragments arranged by size:
7679 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2354 2854 3244
Size: 54 1425 50 275 18 524 500 390
Cuts at: 3244 4171 4636 4977 5192 7435 8324 9953 8
Size: 927 465 341 215 2243 889 1629 43
Fragments arranged by size:
2243 1629 1425 927 889 524 500 465
390 341 275 215 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7435 1537
Size: 275 5623 4090
Fragments arranged by size:
5623 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2822 3415 3891 3991 4085 4933 5123 5205
Size: 1469 593 476 100 94 848 190 82
Cuts at: 5205 5470 5565 5978 6655 6750 7918 1353
Size: 265 95 413 677 95 1168 3423
Fragments arranged by size:
3423 1469 1168 848 677 593 476 413
265 190 100 95 95 94 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5508 5653 7136 8575 8949
Size: 1064 138 435 3484 145 1483 1439 374
Cuts at: 8949 9095 387
Size: 146 1280
Fragments arranged by size:
3484 1483 1439 1280 1064 435 374 146
145 138
EcoICRI GAG'CTC
Cuts at: 3699 6286 3699
Size: 2587 7401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 3795 4162 4423 4526 4697 4707
Size: 393 677 1486 367 261 103 171 10
Cuts at: 4707 4781 5505 5589 7080 7107 8051 8648 9435
Size: 74 724 84 1491 27 944 597 787
Cuts at: 9435 9703 1239
Size: 268 1524
Fragments arranged by size:
1524 1491 1486 944 787 724 677 597
393 367 268 261 171 103 84 74
27 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 7154 2324
Size: 4830 5158
EcoO109I rG'GnC_Cy
Cuts at: 2120 2188 2311 2585 2969 2970 4144 4262 4541
Size: 68 123 274 384 1 1174 118 279
Cuts at: 4541 4597 5727 5843 6614 2120
Size: 56 1130 116 771 5494
Fragments arranged by size:
5494 1174 1130 771 384 279 274 123
118 116 68 56 1
EcoRI G'AATT_C
Cuts at: 3385 4512 7160 7297 7675 3385
Size: 1127 2648 137 378 5698
Fragments arranged by size:
5698 2648 1127 378 137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2306 4434 2306
Size: 2128 7860
FseI GG_CCGG'CC
Cuts at: 4612 4612
Size: 9988
FspI TGC'GCA
Cuts at: 2132 8494 2132
Size: 6362 3626
HaeII r_GCGC'y
Cuts at: 1678 2303 9135 1678
Size: 625 6832 2531
Fragments arranged by size:
6832 2531 625
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2098 2130 2356 2388 2882
Size: 32 135 32 526 32 226 32 494
Cuts at: 2882 2914 4040 4072 4207 4239 4421 4453 4831
Size: 32 1126 32 135 32 182 32 378
Cuts at: 4831 4863 5142 5174 5403 5435 5549 5581 7103
Size: 32 279 32 229 32 114 32 1522
Cuts at: 7103 7135 8629 8661 8703 8735 1373
Size: 32 1494 32 42 32 2626
Fragments arranged by size:
2626 1522 1494 1126 526 494 378 279
229 226 182 135 135 114 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5390 7332 7578 669
Size: 988 281 3452 1942 246 3079
Fragments arranged by size:
3452 3079 1942 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4411 5819 748
Size: 3663 1408 4917
Fragments arranged by size:
4917 3663 1408
HpaI GTT'AAC
Cuts at: 7578 7578
Size: 9988
KasI G'GCGC_C
Cuts at: 2299 2299
Size: 9988
KpnI G_GTAC'C
Cuts at: 4771 6964 7313 4771
Size: 2193 349 7446
Fragments arranged by size:
7446 2193 349
MfeI C'AATT_G
Cuts at: 6205 6771 7587 6205
Size: 566 816 8606
Fragments arranged by size:
8606 816 566
MluI A'CGCG_T
Cuts at: 9864 9864
Size: 9988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5880 6209 6880 9102 9286 1305
Size: 4575 329 671 2222 184 2007
Fragments arranged by size:
4575 2222 2007 671 329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2356 3246 4638 4979 10
Size: 54 1425 343 524 890 1392 341 5019
Fragments arranged by size:
5019 1425 1392 890 524 343 341 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4214 4721 5033 6687 6852 7946 8305
Size: 648 3048 507 312 1654 165 1094 359
Cuts at: 8305 8464 518
Size: 159 2042
Fragments arranged by size:
3048 2042 1654 1094 648 507 359 312
165 159
MspA1I CmG'CkG
Cuts at: 1606 2093 2102 3269 4023 4486 5809 7404 8081
Size: 487 9 1167 754 463 1323 1595 677
Cuts at: 8081 9229 9474 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1595 1323 1167 1148 754 677 487
463 245 9
NaeI GCC'GGC
Cuts at: 2152 4610 5192 2152
Size: 2458 582 6948
Fragments arranged by size:
6948 2458 582
NarI GG'CG_CC
Cuts at: 2300 2300
Size: 9988
NcoI C'CATG_G
Cuts at: 513 1065 2004 4161 513
Size: 552 939 2157 6340
Fragments arranged by size:
6340 2157 939 552
NdeI CA'TA_TG
Cuts at: 387 6410 387
Size: 6023 3965
NgoMIV G'CCGG_C
Cuts at: 2150 4608 5190 2150
Size: 2458 582 6948
Fragments arranged by size:
6948 2458 582
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9988
NotI GC'GGCC_GC
Cuts at: 7435 7435
Size: 9988
NsiI A_TGCA'T
Cuts at: 3593 4718 3593
Size: 1125 8863
NspI r_CATG'y
Cuts at: 1132 1229 2519 3595 5342 7133 7346 8891 1132
Size: 97 1290 1076 1747 1791 213 1545 2229
Fragments arranged by size:
2229 1791 1747 1545 1290 1076 213 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9988
PciI A'CATG_T
Cuts at: 7129 8887 7129
Size: 1758 8230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5056 1108
Size: 3948 6040
PfoI T'CCnGG_A
Cuts at: 6219 6879 6219
Size: 660 9328
PmeI GTTT'AAAC
Cuts at: 7292 7292
Size: 9988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2426 2458 8057 8089 9570 9602 2426
Size: 32 5599 32 1481 32 2812
Fragments arranged by size:
5599 2812 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2188 2311 2585 5727 5843 2188
Size: 123 274 3142 116 6333
Fragments arranged by size:
6333 3142 274 123 116
PshAI GACnn'nnGTC
Cuts at: 1613 6424 1613
Size: 4811 5177
PsiI TTA'TAA
Cuts at: 7558 7558
Size: 9988
PspOMI G'GGCC_C
Cuts at: 2969 4144 4540 6613 2969
Size: 1175 396 2073 6344
Fragments arranged by size:
6344 2073 1175 396
PspXI vC'TCGA_Gb
Cuts at: 2136 2136
Size: 9988
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4101 4133 7208 7240 4101
Size: 32 3075 32 6849
Fragments arranged by size:
6849 3075 32 32
PstI C_TGCA'G
Cuts at: 830 3790 4048 4491 4704 6350 7209 7340 830
Size: 2960 258 443 213 1646 859 131 3478
Fragments arranged by size:
3478 2960 1646 859 443 258 213 131
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9988
PvuII CAG'CTG
Cuts at: 3269 4023 4486 3269
Size: 754 463 8771
Fragments arranged by size:
8771 754 463
SacI G_AGCT'C
Cuts at: 3701 6288 3701
Size: 2587 7401
SacII CC_GC'GG
Cuts at: 2094 2094
Size: 9988
SalI G'TCGA_C
Cuts at: 1936 5388 7330 1936
Size: 3452 1942 4594
Fragments arranged by size:
4594 3452 1942
SanDI GG'GwC_CC
Cuts at: 5727 5843 5727
Size: 116 9872
SapI GCTCTTCn'nnn_
Cuts at: 3891 5565 5978 3891
Size: 1674 413 7901
Fragments arranged by size:
7901 1674 413
SbfI CC_TGCA'GG
Cuts at: 4704 7340 4704
Size: 2636 7352
ScaI AGT'ACT
Cuts at: 8236 9948 8236
Size: 1712 8276
SexAI A'CCwGG_T
Cuts at: 6503 7071 6503
Size: 568 9420
SfcI C'TryA_G
Cuts at: 826 1045 2590 3123 3162 3529 3786 4044 4487
Size: 219 1545 533 39 367 257 258 443
Cuts at: 4487 4700 4806 6346 7205 7336 8471 9152 9343
Size: 213 106 1540 859 131 1135 681 191
Cuts at: 9343 826
Size: 1471
Fragments arranged by size:
1545 1540 1471 1135 859 681 533 443
367 258 257 219 213 191 131 106
39
SfoI GGC'GCC
Cuts at: 2301 2301
Size: 9988
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9988
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9988
SmaI CCC'GGG
Cuts at: 1872 5348 7315 1872
Size: 3476 1967 4545
Fragments arranged by size:
4545 3476 1967
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2136 3686 6275 6458 6764 7256
Size: 197 926 193 1550 2589 183 306 492
Cuts at: 7256 8092 8993 9255 820
Size: 836 901 262 1553
Fragments arranged by size:
2589 1553 1550 926 901 836 492 306
262 197 193 183
SnaBI TAC'GTA
Cuts at: 493 7779 493
Size: 7286 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9988
SphI G_CATG'C
Cuts at: 1132 7346 1132
Size: 6214 3774
SspI AAT'ATT
Cuts at: 5 52 5323 7912 5
Size: 47 5271 2589 2081
Fragments arranged by size:
5271 2589 2081 47
StuI AGG'CCT
Cuts at: 1517 3374 3705 4503 5632 6604 1517
Size: 1857 331 798 1129 972 4901
Fragments arranged by size:
4901 1857 1129 972 798 331
StyI C'CwwG_G
Cuts at: 513 1065 2004 4008 4161 4602 5928 6861 7033
Size: 552 939 2004 153 441 1326 933 172
Cuts at: 7033 7427 513
Size: 394 3074
Fragments arranged by size:
3074 2004 1326 939 933 552 441 394
172 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2874 3188 4033 5207 8015 8032 8185
Size: 1255 1294 314 845 1174 2808 17 153
Cuts at: 8185 8370 8815 325
Size: 185 445 1498
Fragments arranged by size:
2808 1498 1294 1255 1174 845 445 314
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6624 8234 9946
Size: 274 80 33 51 1443 4647 1610 1712
Cuts at: 9946 96
Size: 138
Fragments arranged by size:
4647 1712 1610 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2627 2659 2854 2870 2886 2902 2977 3009 3793
Size: 32 195 16 16 16 75 32 784
Cuts at: 3793 3813 3825 3845 9948 9980 2627
Size: 20 12 20 6103 32 2635
Fragments arranged by size:
6103 2635 784 195 75 32 32 32
20 20 16 16 16 12
Tth111I GACn'n_nGTC
Cuts at: 1316 2315 1316
Size: 999 8989
XbaI T'CTAG_A
Cuts at: 4954 7324 4954
Size: 2370 7618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5035 6840 5035
Size: 1805 8183
XhoI C'TCGA_G
Cuts at: 1943 2136 1943
Size: 193 9795
XmaI C'CCGG_G
Cuts at: 1870 5346 7313 1870
Size: 3476 1967 4545
Fragments arranged by size:
4545 3476 1967
XmnI GAAnn'nnTTC
Cuts at: 2421 4391 6215 6483 8117 2421
Size: 1970 1824 268 1634 4292
Fragments arranged by size:
4292 1970 1824 1634 268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7796 276
Size: 53 83 186 936 6262 2468
Fragments arranged by size:
6262 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BsiWI BsmBI BstZ17I EcoRV
FspAI NruI PacI PmlI RsrII SfiI SrfI SwaI