Restriction map for
pFN21AA1081
(Circular) (Six-base) MAPSORT of: pFN21AA1081.seq Check: 9652 from: 1 to: 7681
pFN21AA1081 7681 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2296 5491 278
Size: 53 83 186 936 760 3195 2468
Fragments arranged by size:
3195 2468 936 760 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5024 1937
Size: 3087 4594
Acc65I G'GTAC_C
Cuts at: 5002 5002
Size: 7681
AclI AA'CG_TT
Cuts at: 1563 5808 6181 1563
Size: 4245 373 3063
Fragments arranged by size:
4245 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 2606 3588 3632 4340 4835 5744 7128
Size: 866 108 982 44 708 495 909 1384
Cuts at: 7128 7396 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 982 909 866 708 495 268
108 44
AflII C'TTAA_G
Cuts at: 820 1017 4007 4847 820
Size: 197 2990 840 3654
Fragments arranged by size:
3654 2990 840 197
AflIII A'CryG_T
Cuts at: 1203 3612 6580 7557 1203
Size: 2409 2968 977 1327
Fragments arranged by size:
2968 2409 1327 977
AgeI A'CCGG_T
Cuts at: 6512 6512
Size: 7681
AhdI GACnn_n'nnGTC
Cuts at: 6410 6410
Size: 7681
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7681
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3486 1184
Size: 2302 5379
AlwNI CAG_nnn'CTG
Cuts at: 3226 3343 4636 5424 6996 3226
Size: 117 1293 788 1572 3911
Fragments arranged by size:
3911 1572 1293 788 117
ApaLI G'TGCA_C
Cuts at: 4353 5738 6894 4353
Size: 1385 1156 5140
Fragments arranged by size:
5140 1385 1156
ApoI r'AATT_y
Cuts at: 1493 3174 3216 3305 3552 4990 5216 5368 7477
Size: 1681 42 89 247 1438 226 152 2109
Cuts at: 7477 1493
Size: 1697
Fragments arranged by size:
2109 1697 1681 1438 247 226 152 89
42
AseI AT'TA_AT
Cuts at: 160 1032 6235 160
Size: 872 5203 1606
Fragments arranged by size:
5203 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7681
AvaI C'yCGr_G
Cuts at: 1870 1943 2916 5006 1870
Size: 73 973 2090 4545
Fragments arranged by size:
4545 2090 973 73
BamHI G'GATC_C
Cuts at: 2715 5011 5378 2715
Size: 2296 367 5018
Fragments arranged by size:
5018 2296 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5002 6457 618
Size: 325 217 627 3215 1455 1842
Fragments arranged by size:
3215 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2057 2579 2602 5000 1391
Size: 666 522 23 2398 4072
Fragments arranged by size:
4072 2398 666 522 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2994 4796 928
Size: 971 1095 1802 3813
Fragments arranged by size:
3813 1802 1095 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5872 5906 1903
Size: 34 3935 34 3678
Fragments arranged by size:
3935 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5573 6783 5573
Size: 1210 6471
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7681
BfrBI ATG'CAT
Cuts at: 3097 3097
Size: 7681
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6292 136
Size: 107 122 71 631 605 4620 1525
Fragments arranged by size:
4620 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3877 7676 3877
Size: 3799 3882
BlpI GC'TnA_GC
Cuts at: 2820 5098 2820
Size: 2278 5403
Bme1580I G_kGCm'C
Cuts at: 1790 2020 4357 5742 6898 1790
Size: 230 2337 1385 1156 2573
Fragments arranged by size:
2573 2337 1385 1156 230
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7681
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5412 6370 442
Size: 416 53 83 399 24 3995 958 1753
Fragments arranged by size:
3995 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2349 4514 1056
Size: 1293 2165 4223
Fragments arranged by size:
4223 2165 1293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2593 2684 3609 4724 6341 1239
Size: 1354 91 925 1115 1617 2579
Fragments arranged by size:
2579 1617 1354 1115 925 91
Bpu10I CC'TnA_GC
Cuts at: 3456 3456
Size: 7681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3590 4264 5806 6671 6969 3590
Size: 674 1542 865 298 4302
Fragments arranged by size:
4302 1542 865 674 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3195 6344 882
Size: 617 1696 3149 2219
Fragments arranged by size:
3149 2219 1696 617
BsaAI yAC'GTr
Cuts at: 493 1204 5472 493
Size: 711 4268 2702
Fragments arranged by size:
4268 2702 711
BsaBI GATnn'nnATC
Cuts at: 2687 7675 2687
Size: 4988 2693
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2293 4749 5488 5870
Size: 53 83 186 936 760 2456 739 382
Cuts at: 5870 6505 275
Size: 635 1451
Fragments arranged by size:
2456 1451 936 760 739 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6114 6512 6786 6933 7344 6114
Size: 398 274 147 411 6451
Fragments arranged by size:
6451 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4760 4790 4928 4958 1178
Size: 30 165 30 3357 30 138 30 3901
Fragments arranged by size:
3901 3357 165 138 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2810 3104 3791 4058 4382 4571 4976 4979
Size: 1624 294 687 267 324 189 405 3
Cuts at: 4979 1186
Size: 3888
Fragments arranged by size:
3888 1624 687 405 324 294 267 189
3
BseYI C'CCAG_C
Cuts at: 4626 6884 7380 4626
Size: 2258 496 4927
Fragments arranged by size:
4927 2258 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5131 5892 6920 1540
Size: 275 186 3130 761 1028 2301
Fragments arranged by size:
3130 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4357 5742 5827 6898 4357
Size: 1385 85 1071 5140
Fragments arranged by size:
5140 1385 1071 85
BsmI GAATG_Cn'
Cuts at: 3118 5192 5285 3118
Size: 2074 93 5514
Fragments arranged by size:
5514 2074 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2057 2579 2602 4357 5000 5742
Size: 399 230 37 522 23 1755 643 742
Cuts at: 5742 5827 6898 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1755 1071 742 643 522 399 230
85 37 23
BspEI T'CCGG_A
Cuts at: 7344 7344
Size: 7681
BspHI T'CATG_A
Cuts at: 5570 7300 5570
Size: 1730 5951
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5036 844
Size: 4192 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2022 2830 3631 3799 4498 5568 1394
Size: 429 199 808 801 168 699 1070 3507
Fragments arranged by size:
3507 1070 808 801 699 429 199 168
BsrDI GCAATG_nn'
Cuts at: 65 1248 6176 6350 65
Size: 1183 4928 174 1396
Fragments arranged by size:
4928 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6325 6512 1700
Size: 110 4515 187 2869
Fragments arranged by size:
4515 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2656 4480 96
Size: 2560 1824 3297
Fragments arranged by size:
3297 2560 1824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7681
BssSI C'ACGA_G
Cuts at: 5741 6753 5741
Size: 1012 6669
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7681
BstBI TT'CG_AA
Cuts at: 2857 6485 2857
Size: 3628 4053
BstEII G'GTnAC_C
Cuts at: 1184 2096 1184
Size: 912 6769
BstXI CCAn_nnnn'nTGG
Cuts at: 2057 2057
Size: 7681
BstYI r'GATC_y
Cuts at: 1972 2461 2715 3877 3949 5011 5378 5763 5780
Size: 489 254 1162 72 1062 367 385 17
Cuts at: 5780 7232 7318 7330 7676 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1162 1062 489 385 367 346
254 86 72 17 12
Bsu36I CC'TnA_GG
Cuts at: 3982 3982
Size: 7681
BtgI C'CryG_G
Cuts at: 513 1065 4572 513
Size: 552 3507 3622
Fragments arranged by size:
3622 3507 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6942
Fragments arranged by size:
6942 683 56
BtsI GCAGTG_nn'
Cuts at: 2143 2158 2356 3756 5197 5991 6011 2143
Size: 15 198 1400 1441 794 20 3813
Fragments arranged by size:
3813 1441 1400 794 198 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7681
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7646
DraI TTT'AAA
Cuts at: 2190 4985 2190
Size: 2795 4886
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6688 809
Size: 507 5372 1802
Fragments arranged by size:
5372 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2278 3325 4569
Size: 54 1425 50 275 18 448 1047 1244
Cuts at: 4569 4881 5128 6017 7646 8
Size: 312 247 889 1629 43
Fragments arranged by size:
1629 1425 1244 1047 889 448 312 275
247 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5128 1537
Size: 275 3316 4090
Fragments arranged by size:
4090 3316 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3204 3510 3564 4343 4446 4728 5611 1353
Size: 1851 306 54 779 103 282 883 3423
Fragments arranged by size:
3423 1851 883 779 306 282 103 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6268 6642 6788 387
Size: 1064 138 4679 374 146 1280
Fragments arranged by size:
4679 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2498 2593 2606 2684 3588 3609 3632
Size: 393 866 95 13 78 904 21 23
Cuts at: 3632 4340 4724 4835 5744 6341 7128 7396 1239
Size: 708 384 111 909 597 787 268 1524
Fragments arranged by size:
1524 909 904 866 787 708 597 393
384 268 111 95 78 23 21 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2435 3209 2435
Size: 774 6907
EcoO109I rG'GnC_Cy
Cuts at: 2725 3781 2725
Size: 1056 6625
EcoRI G'AATT_C
Cuts at: 4990 5368 4990
Size: 378 7303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3463 3695 3938 4739 3463
Size: 232 243 801 6405
Fragments arranged by size:
6405 801 243 232
FspI TGC'GCA
Cuts at: 6187 6187
Size: 7681
HaeII r_GCGC'y
Cuts at: 1678 6828 1678
Size: 5150 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3649 3681 4760 4792 4849
Size: 32 135 32 2077 32 1079 32 57
Cuts at: 4849 4881 6322 6354 6396 6428 1373
Size: 32 1441 32 42 32 2626
Fragments arranged by size:
2626 2077 1441 1079 135 57 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5025 5271 669
Size: 988 281 3087 246 3079
Fragments arranged by size:
3087 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2616 2936 748
Size: 1868 320 5493
Fragments arranged by size:
5493 1868 320
HpaI GTT'AAC
Cuts at: 5271 5271
Size: 7681
KpnI G_GTAC'C
Cuts at: 5006 5006
Size: 7681
MfeI C'AATT_G
Cuts at: 3804 4067 5280 3804
Size: 263 1213 6205
Fragments arranged by size:
6205 1213 263
MluI A'CGCG_T
Cuts at: 7557 7557
Size: 7681
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2122 3597 4245 6795 6979 1305
Size: 817 1475 648 2550 184 2007
Fragments arranged by size:
2550 2007 1475 817 648 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3327 4571 10
Size: 54 1425 343 1495 1244 3120
Fragments arranged by size:
3120 1495 1425 1244 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 2456 2681 3431 5639 5998 6157
Size: 648 1072 218 225 750 2208 359 159
Cuts at: 6157 518
Size: 2042
Fragments arranged by size:
2208 2042 1072 750 648 359 225 218
159
MspA1I CmG'CkG
Cuts at: 1606 2476 2737 5097 5774 6922 7167 1606
Size: 870 261 2360 677 1148 245 2120
Fragments arranged by size:
2360 2120 1148 870 677 261 245
NcoI C'CATG_G
Cuts at: 513 1065 4572 513
Size: 552 3507 3622
Fragments arranged by size:
3622 3507 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7681
NheI G'CTAG_C
Cuts at: 1052 2345 4510 1052
Size: 1293 2165 4223
Fragments arranged by size:
4223 2165 1293
NotI GC'GGCC_GC
Cuts at: 5128 5128
Size: 7681
NsiI A_TGCA'T
Cuts at: 3099 3099
Size: 7681
NspI r_CATG'y
Cuts at: 1132 1229 2680 3616 3965 5039 6584 1132
Size: 97 1451 936 349 1074 1545 2229
Fragments arranged by size:
2229 1545 1451 1074 936 349 97
PasI CC'CwG_GG
Cuts at: 1348 2057 1348
Size: 709 6972
PciI A'CATG_T
Cuts at: 3612 6580 3612
Size: 2968 4713
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7681
PfoI T'CCnGG_A
Cuts at: 2700 2700
Size: 7681
PmeI GTTT'AAAC
Cuts at: 4985 4985
Size: 7681
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5750 5782 7263 7295 5750
Size: 32 1481 32 6136
Fragments arranged by size:
6136 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 2725
Size: 7681
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7681
PsiI TTA'TAA
Cuts at: 5251 5251
Size: 7681
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2098 2130 2098
Size: 32 7649
PstI C_TGCA'G
Cuts at: 830 2469 3378 3483 4495 5033 830
Size: 1639 909 105 1012 538 3478
Fragments arranged by size:
3478 1639 1012 909 538 105
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7681
PvuII CAG'CTG
Cuts at: 2476 2737 2476
Size: 261 7420
SalI G'TCGA_C
Cuts at: 1936 5023 1936
Size: 3087 4594
SapI GCTCTTCn'nnn_
Cuts at: 4446 4728 4446
Size: 282 7399
SbfI CC_TGCA'GG
Cuts at: 5033 5033
Size: 7681
ScaI AGT'ACT
Cuts at: 3250 5929 7641 3250
Size: 2679 1712 3290
Fragments arranged by size:
3290 2679 1712
SexAI A'CCwGG_T
Cuts at: 4154 4154
Size: 7681
SfcI C'TryA_G
Cuts at: 826 1045 2465 3374 3479 4415 4491 5029 6164
Size: 219 1420 909 105 936 76 538 1135
Cuts at: 6164 6845 7036 826
Size: 681 191 1471
Fragments arranged by size:
1471 1420 1135 936 909 681 538 219
191 105 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7681
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7681
SmaI CCC'GGG
Cuts at: 1872 5008 1872
Size: 3136 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3605 4007 4279 4847 5785 6686
Size: 197 926 1662 402 272 568 938 901
Cuts at: 6686 6948 820
Size: 262 1553
Fragments arranged by size:
1662 1553 938 926 901 568 402 272
262 197
SnaBI TAC'GTA
Cuts at: 493 5472 493
Size: 4979 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7681
SphI G_CATG'C
Cuts at: 1132 3965 5039 1132
Size: 2833 1074 3774
Fragments arranged by size:
3774 2833 1074
SspI AAT'ATT
Cuts at: 5 52 5605 5
Size: 47 5553 2081
Fragments arranged by size:
5553 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7681
StyI C'CwwG_G
Cuts at: 513 1065 2973 4572 5120 513
Size: 552 1908 1599 548 3074
Fragments arranged by size:
3074 1908 1599 552 548
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2083 4140 4640 5708 5725 5878 6063
Size: 1255 503 2057 500 1068 17 153 185
Cuts at: 6063 6508 325
Size: 445 1498
Fragments arranged by size:
2057 1498 1255 1068 503 500 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2646 2656 3248
Size: 274 80 33 51 1443 669 10 592
Cuts at: 3248 4480 5927 7639 96
Size: 1232 1447 1712 138
Fragments arranged by size:
1712 1447 1443 1232 669 592 274 138
80 51 33 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4895 4927 4928 4960 7641 7673 4895
Size: 32 1 32 2681 32 4903
Fragments arranged by size:
4903 2681 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7681
XbaI T'CTAG_A
Cuts at: 5017 5017
Size: 7681
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7681
XmaI C'CCGG_G
Cuts at: 1870 5006 1870
Size: 3136 4545
XmnI GAAnn'nnTTC
Cuts at: 3666 4722 5810 3666
Size: 1056 1088 5537
Fragments arranged by size:
5537 1088 1056
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2294 5489 276
Size: 53 83 186 936 760 3195 2468
Fragments arranged by size:
3195 2468 936 760 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI