Restriction map for
pFN21AA1070
(Circular) (Six-base) MAPSORT of: pFN21AA1070.seq Check: 5150 from: 1 to: 7174
pFN21AA1070 7174 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4984 278
Size: 53 83 186 936 3448 2468
Fragments arranged by size:
3448 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 3869 4517 1937
Size: 1386 546 648 4594
Fragments arranged by size:
4594 1386 648 546
Acc65I G'GTAC_C
Cuts at: 3832 4495 3832
Size: 663 6511
AclI AA'CG_TT
Cuts at: 1563 5301 5674 1563
Size: 3738 373 3063
Fragments arranged by size:
3738 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 2853 3303 5237 6621 6889 1632
Size: 470 751 450 1934 1384 268 1917
Fragments arranged by size:
1934 1917 1384 751 470 450 268
AflII C'TTAA_G
Cuts at: 820 1017 2761 820
Size: 197 1744 5233
Fragments arranged by size:
5233 1744 197
AflIII A'CryG_T
Cuts at: 1203 6073 7050 1203
Size: 4870 977 1327
Fragments arranged by size:
4870 1327 977
AgeI A'CCGG_T
Cuts at: 6005 6005
Size: 7174
AhdI GACnn_n'nnGTC
Cuts at: 4275 5903 4275
Size: 1628 5546
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7174
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7174
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3513 3545 3513
Size: 32 7142
AlwNI CAG_nnn'CTG
Cuts at: 4917 6489 4917
Size: 1572 5602
ApaLI G'TGCA_C
Cuts at: 5231 6387 5231
Size: 1156 6018
ApoI r'AATT_y
Cuts at: 1493 2220 2572 3185 3253 3637 3686 3928 4483
Size: 727 352 613 68 384 49 242 555
Cuts at: 4483 4709 4861 6970 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 727 613 555 384 352 242
226 152 68 49
AseI AT'TA_AT
Cuts at: 160 1032 5728 160
Size: 872 4696 1606
Fragments arranged by size:
4696 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7174
AvaI C'yCGr_G
Cuts at: 1870 1943 4499 1870
Size: 73 2556 4545
Fragments arranged by size:
4545 2556 73
BamHI G'GATC_C
Cuts at: 4504 4871 4504
Size: 367 6807
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 3403 3832 4495 5950
Size: 325 217 627 294 1322 429 663 1455
Cuts at: 5950 618
Size: 1842
Fragments arranged by size:
1842 1455 1322 663 627 429 325 294
217
BanII G_rGCy'C
Cuts at: 1391 4493 1391
Size: 3102 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2463 928
Size: 971 564 5639
Fragments arranged by size:
5639 971 564
BbvCI CC'TCA_GC
Cuts at: 4146 4146
Size: 7174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2596 2630 2657 2691 5365 5399 1903
Size: 34 659 34 27 34 2674 34 3678
Fragments arranged by size:
3678 2674 659 34 34 34 34 27
BciVI GTATCCnnnnn_n'
Cuts at: 5066 6276 5066
Size: 1210 5964
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7174
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5785 136
Size: 107 122 71 631 605 4113 1525
Fragments arranged by size:
4113 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7169 7169
Size: 7174
BlpI GC'TnA_GC
Cuts at: 4591 4591
Size: 7174
Bme1580I G_kGCm'C
Cuts at: 1790 2086 5235 6391 1790
Size: 296 3149 1156 2573
Fragments arranged by size:
3149 2573 1156 296
BmgBI CAC'GTC
Cuts at: 1318 3945 1318
Size: 2627 4547
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3340 3551 4905
Size: 416 53 83 399 24 1923 211 1354
Cuts at: 4905 5863 442
Size: 958 1753
Fragments arranged by size:
1923 1753 1354 958 416 399 211 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3155
Size: 7174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2280 4428 5834 1239
Size: 1041 2148 1406 2579
Fragments arranged by size:
2579 2148 1406 1041
Bpu10I CC'TnA_GC
Cuts at: 4146 4146
Size: 7174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2677 3660 5299 6164 6462 2677
Size: 983 1639 865 298 3389
Fragments arranged by size:
3389 1639 983 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5837 882
Size: 617 4338 2219
Fragments arranged by size:
4338 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2025 3453 4965 493
Size: 711 821 1428 1512 2702
Fragments arranged by size:
2702 1512 1428 821 711
BsaBI GATnn'nnATC
Cuts at: 2618 4224 7168 2618
Size: 1606 2944 2624
Fragments arranged by size:
2944 2624 1606
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4981 5363 5998 275
Size: 53 83 186 936 3448 382 635 1451
Fragments arranged by size:
3448 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5607 6005 6279 6426 6837 5607
Size: 398 274 147 411 5944
Fragments arranged by size:
5944 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3513 3543 3744 3774 1178
Size: 30 165 30 2110 30 201 30 4578
Fragments arranged by size:
4578 2110 201 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4129 4318 1186
Size: 2943 189 4042
Fragments arranged by size:
4042 2943 189
BseYI C'CCAG_C
Cuts at: 2789 2910 6377 6873 2789
Size: 121 3467 496 3090
Fragments arranged by size:
3467 3090 496 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3633 1163
Size: 573 1897 4704
Fragments arranged by size:
4704 1897 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4624 5385 6413 1540
Size: 275 186 2623 761 1028 2301
Fragments arranged by size:
2623 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5235 5320 6391 5235
Size: 85 1071 6018
Fragments arranged by size:
6018 1071 85
BsmI GAATG_Cn'
Cuts at: 3005 4685 4778 3005
Size: 1680 93 5401
Fragments arranged by size:
5401 1680 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3516 3516
Size: 7174
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 4493 5235 5320 6391 1391
Size: 399 296 2407 742 85 1071 2174
Fragments arranged by size:
2407 2174 1071 742 399 296 85
BspEI T'CCGG_A
Cuts at: 6837 6837
Size: 7174
BspHI T'CATG_A
Cuts at: 4228 5063 6793 4228
Size: 835 1730 4609
Fragments arranged by size:
4609 1730 835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2817 2938 4529 844
Size: 1973 121 1591 3489
Fragments arranged by size:
3489 1973 1591 121
BsrBI CCG'CTC
Cuts at: 1394 1823 5061 1394
Size: 429 3238 3507
Fragments arranged by size:
3507 3238 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2614 2977 3475 5669 5843 65
Size: 1183 1366 363 498 2194 174 1396
Fragments arranged by size:
2194 1396 1366 1183 498 363 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5818 6005 1700
Size: 110 4008 187 2869
Fragments arranged by size:
4008 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7174
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7174
BssSI C'ACGA_G
Cuts at: 5234 6246 5234
Size: 1012 6162
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2939 794
Size: 2145 5029
BstBI TT'CG_AA
Cuts at: 5978 5978
Size: 7174
BstEII G'GTnAC_C
Cuts at: 1184 2753 2837 3653 4308 1184
Size: 1569 84 816 655 4050
Fragments arranged by size:
4050 1569 816 655 84
BstXI CCAn_nnnn'nTGG
Cuts at: 2313 2313
Size: 7174
BstYI r'GATC_y
Cuts at: 1972 2780 3424 4504 4871 5256 5273 6725 6811
Size: 808 644 1080 367 385 17 1452 86
Cuts at: 6811 6823 7169 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1080 808 644 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3324 3870 3324
Size: 546 6628
BtgI C'CryG_G
Cuts at: 513 1065 2004 2542 3517 513
Size: 552 939 538 975 4170
Fragments arranged by size:
4170 975 939 552 538
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3225 487
Size: 683 56 1999 4436
Fragments arranged by size:
4436 1999 683 56
BtsI GCAGTG_nn'
Cuts at: 2009 2055 3957 4058 4690 5484 5504 2009
Size: 46 1902 101 632 794 20 3679
Fragments arranged by size:
3679 1902 794 632 101 46 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7174
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2323 2358 2944 2979 529
Size: 35 1759 35 586 35 4724
Fragments arranged by size:
4724 1759 586 35 35 35
DraI TTT'AAA
Cuts at: 2472 4478 2472
Size: 2006 5168
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6181 809
Size: 507 4865 1802
Fragments arranged by size:
4865 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 2125 2952
Size: 54 1425 50 275 18 197 98 827
Cuts at: 2952 4621 5510 7139 8
Size: 1669 889 1629 43
Fragments arranged by size:
1669 1629 1425 889 827 275 197 98
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4621 1537
Size: 275 2809 4090
Fragments arranged by size:
4090 2809 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4179 5104 1353
Size: 2826 925 3423
Fragments arranged by size:
3423 2826 925
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5761 6135 6281 387
Size: 1064 138 4172 374 146 1280
Fragments arranged by size:
4172 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2280 2853 3303 4428 5237 5834
Size: 393 470 178 573 450 1125 809 597
Cuts at: 5834 6621 6889 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1125 809 787 597 573 470 450
393 268 178
EcoO109I rG'GnC_Cy
Cuts at: 2228 2516 2228
Size: 288 6886
EcoRI G'AATT_C
Cuts at: 4483 4861 4483
Size: 378 6796
EcoRV GAT'ATC
Cuts at: 2323 3278 2323
Size: 955 6219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2411 3770 2411
Size: 1359 5815
FspI TGC'GCA
Cuts at: 5680 5680
Size: 7174
HaeII r_GCGC'y
Cuts at: 1678 6321 1678
Size: 4643 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3206 3238 3416 3448 4261
Size: 32 135 32 1634 32 178 32 813
Cuts at: 4261 4293 5815 5847 5889 5921 1373
Size: 32 1522 32 42 32 2626
Fragments arranged by size:
2626 1634 1522 813 178 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2629 2806 3034 4518 4764 669
Size: 988 281 691 177 228 1484 246 3079
Fragments arranged by size:
3079 1484 988 691 281 246 228 177
HindIII A'AGCT_T
Cuts at: 748 2712 748
Size: 1964 5210
HpaI GTT'AAC
Cuts at: 4764 4764
Size: 7174
KpnI G_GTAC'C
Cuts at: 3836 4499 3836
Size: 663 6511
MfeI C'AATT_G
Cuts at: 4773 4773
Size: 7174
MluI A'CGCG_T
Cuts at: 7050 7050
Size: 7174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2825 3804 4030 6288 6472 1305
Size: 1520 979 226 2258 184 2007
Fragments arranged by size:
2258 2007 1520 979 226 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2029 2127 2954 10
Size: 54 1425 343 197 98 827 4230
Fragments arranged by size:
4230 1425 827 343 197 98 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4233 4251 4349 5132 5491 5650 518
Size: 648 3067 18 98 783 359 159 2042
Fragments arranged by size:
3067 2042 783 648 359 159 98 18
MspA1I CmG'CkG
Cuts at: 1606 2910 3410 3422 4590 5267 6415 6660 1606
Size: 1304 500 12 1168 677 1148 245 2120
Fragments arranged by size:
2120 1304 1168 1148 677 500 245 12
NcoI C'CATG_G
Cuts at: 513 1065 2004 2542 513
Size: 552 939 538 5145
Fragments arranged by size:
5145 939 552 538
NdeI CA'TA_TG
Cuts at: 387 2257 2437 387
Size: 1870 180 5124
Fragments arranged by size:
5124 1870 180
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7174
NotI GC'GGCC_GC
Cuts at: 4621 4621
Size: 7174
NspI r_CATG'y
Cuts at: 1132 1229 2061 4532 6077 1132
Size: 97 832 2471 1545 2229
Fragments arranged by size:
2471 2229 1545 832 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7174
PciI A'CATG_T
Cuts at: 6073 6073
Size: 7174
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7174
PmeI GTTT'AAAC
Cuts at: 4478 4478
Size: 7174
PmlI CAC'GTG
Cuts at: 2025 2025
Size: 7174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3513 3545 5243 5275 6756 6788 3513
Size: 32 1698 32 1481 32 3899
Fragments arranged by size:
3899 1698 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2516 2516
Size: 7174
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7174
PsiI TTA'TAA
Cuts at: 4744 4744
Size: 7174
PstI C_TGCA'G
Cuts at: 830 4526 830
Size: 3696 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7174
PvuII CAG'CTG
Cuts at: 2910 3410 2910
Size: 500 6674
RsrII CG'GwC_CG
Cuts at: 4269 4269
Size: 7174
SalI G'TCGA_C
Cuts at: 1936 4516 1936
Size: 2580 4594
SanDI GG'GwC_CC
Cuts at: 2516 2516
Size: 7174
SbfI CC_TGCA'GG
Cuts at: 4526 4526
Size: 7174
ScaI AGT'ACT
Cuts at: 2646 5422 7134 2646
Size: 2776 1712 2686
Fragments arranged by size:
2776 2686 1712
SfcI C'TryA_G
Cuts at: 826 1045 3005 3411 3564 4522 5657 6338 6529
Size: 219 1960 406 153 958 1135 681 191
Cuts at: 6529 826
Size: 1471
Fragments arranged by size:
1960 1471 1135 958 681 406 219 191
153
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7174
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7174
SmaI CCC'GGG
Cuts at: 1872 4501 1872
Size: 2629 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2656 2761 3675 5278 6179 6441
Size: 197 926 713 105 914 1603 901 262
Cuts at: 6441 820
Size: 1553
Fragments arranged by size:
1603 1553 926 914 901 713 262 197
105
SnaBI TAC'GTA
Cuts at: 493 3453 4965 493
Size: 2960 1512 2702
Fragments arranged by size:
2960 2702 1512
SpeI A'CTAG_T
Cuts at: 152 2726 152
Size: 2574 4600
SphI G_CATG'C
Cuts at: 1132 2061 4532 1132
Size: 929 2471 3774
Fragments arranged by size:
3774 2471 929
SspI AAT'ATT
Cuts at: 5 52 5098 5
Size: 47 5046 2081
Fragments arranged by size:
5046 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7174
StyI C'CwwG_G
Cuts at: 513 1065 2004 2542 3169 4613 513
Size: 552 939 538 627 1444 3074
Fragments arranged by size:
3074 1444 939 627 552 538
SwaI ATTT'AAAT
Cuts at: 2472 2472
Size: 7174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 3388 4351 5201 5218 5371 5556
Size: 1255 489 1319 963 850 17 153 185
Cuts at: 5556 6001 325
Size: 445 1498
Fragments arranged by size:
1498 1319 1255 963 850 489 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2644 2660 3538
Size: 274 80 33 51 1443 667 16 878
Cuts at: 3538 4095 5420 7132 96
Size: 557 1325 1712 138
Fragments arranged by size:
1712 1443 1325 878 667 557 274 138
80 51 33 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3380 3412 7134 7166 3380
Size: 32 3722 32 3388
Fragments arranged by size:
3722 3388 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7174
XbaI T'CTAG_A
Cuts at: 4510 4510
Size: 7174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2037 2310 2527 3068 2037
Size: 273 217 541 6143
Fragments arranged by size:
6143 541 273 217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7174
XmaI C'CCGG_G
Cuts at: 1870 4499 1870
Size: 2629 4545
XmnI GAAnn'nnTTC
Cuts at: 3928 5303 3928
Size: 1375 5799
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4982 276
Size: 53 83 186 936 3448 2468
Fragments arranged by size:
3448 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
RsrII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI
PspXI PsrI SacI SacII SapI SexAI SfiI SfoI
SrfI