Restriction map for
pFN21AA1042
(Circular) (Six-base) MAPSORT of: pFN21AA1042.seq Check: 8884 from: 1 to: 7564
pFN21AA1042 7564 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2452 2871 2452
Size: 419 7145
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5374 278
Size: 53 83 186 936 3838 2468
Fragments arranged by size:
3838 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4907 1937
Size: 2970 4594
Acc65I G'GTAC_C
Cuts at: 4672 4885 4672
Size: 213 7351
AclI AA'CG_TT
Cuts at: 1563 5691 6064 1563
Size: 4128 373 3063
Fragments arranged by size:
4128 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 2726 3065 3679 4026 5627 7011 7279
Size: 881 213 339 614 347 1601 1384 268
Cuts at: 7279 1632
Size: 1917
Fragments arranged by size:
1917 1601 1384 881 614 347 339 268
213
AfeI AGC'GCT
Cuts at: 2545 2545
Size: 7564
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7367
AflIII A'CryG_T
Cuts at: 1203 4509 6463 7440 1203
Size: 3306 1954 977 1327
Fragments arranged by size:
3306 1954 1327 977
AgeI A'CCGG_T
Cuts at: 6395 6395
Size: 7564
AhdI GACnn_n'nnGTC
Cuts at: 6293 6293
Size: 7564
AleI CACnn'nnGTG
Cuts at: 1166 4127 4772 1166
Size: 2961 645 3958
Fragments arranged by size:
3958 2961 645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2920 3035 3176 1184
Size: 1736 115 141 5572
Fragments arranged by size:
5572 1736 141 115
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 7532
AlwNI CAG_nnn'CTG
Cuts at: 2050 2746 2938 3583 5307 6879 2050
Size: 696 192 645 1724 1572 2735
Fragments arranged by size:
2735 1724 1572 696 645 192
ApaI G_GGCC'C
Cuts at: 4635 4635
Size: 7564
ApaLI G'TGCA_C
Cuts at: 4405 5621 6777 4405
Size: 1216 1156 5192
Fragments arranged by size:
5192 1216 1156
ApoI r'AATT_y
Cuts at: 1493 4873 5099 5251 7360 1493
Size: 3380 226 152 2109 1697
Fragments arranged by size:
3380 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6118 160
Size: 872 5086 1606
Fragments arranged by size:
5086 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7564
AvaI C'yCGr_G
Cuts at: 1870 1943 2564 4056 4636 4816 4889 1870
Size: 73 621 1492 580 180 73 4545
Fragments arranged by size:
4545 1492 621 580 180 73 73
BamHI G'GATC_C
Cuts at: 4894 5261 4894
Size: 367 7197
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3467 4672 4885 6340 618
Size: 325 217 627 1680 1205 213 1455 1842
Fragments arranged by size:
1842 1680 1455 1205 627 325 217 213
BanII G_rGCy'C
Cuts at: 1391 2044 2565 4053 4224 4301 4415 4588 4635
Size: 653 521 1488 171 77 114 173 47
Cuts at: 4635 4883 1391
Size: 248 4072
Fragments arranged by size:
4072 1488 653 521 248 173 171 114
77 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2714 2828 928
Size: 971 815 114 5664
Fragments arranged by size:
5664 971 815 114
BbvCI CC'TCA_GC
Cuts at: 2771 3500 3964 2771
Size: 729 464 6371
Fragments arranged by size:
6371 729 464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5755 5789 1903
Size: 34 3818 34 3678
Fragments arranged by size:
3818 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5456 6666 5456
Size: 1210 6354
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7564
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4639 6175 136
Size: 107 122 71 631 605 2967 1536 1525
Fragments arranged by size:
2967 1536 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3251 7559 3251
Size: 4308 3256
BlpI GC'TnA_GC
Cuts at: 2043 4836 4981 2043
Size: 2793 145 4626
Fragments arranged by size:
4626 2793 145
Bme1580I G_kGCm'C
Cuts at: 1790 3470 3650 4409 4635 5625 6781 1790
Size: 1680 180 759 226 990 1156 2573
Fragments arranged by size:
2573 1680 1156 990 759 226 180
BmgBI CAC'GTC
Cuts at: 1318 3081 4510 1318
Size: 1763 1429 4372
Fragments arranged by size:
4372 1763 1429
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5295 6253 442
Size: 416 53 83 399 24 3878 958 1753
Fragments arranged by size:
3878 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7564
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 7564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2217 2474 3011 3168 3509 3560 3710
Size: 926 52 257 537 157 341 51 150
Cuts at: 3710 3927 4074 6224 1239
Size: 217 147 2150 2579
Fragments arranged by size:
2579 2150 926 537 341 257 217 157
150 147 52 51
Bpu10I CC'TnA_GC
Cuts at: 2771 2949 3500 3964 2771
Size: 178 551 464 6371
Fragments arranged by size:
6371 551 464 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 5689 6554 6852 2381
Size: 3308 865 298 3093
Fragments arranged by size:
3308 3093 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2708 3953 6227 882
Size: 617 1209 1245 2274 2219
Fragments arranged by size:
2274 2219 1245 1209 617
BsaAI yAC'GTr
Cuts at: 493 1204 5355 493
Size: 711 4151 2702
Fragments arranged by size:
4151 2702 711
BsaBI GATnn'nnATC
Cuts at: 7558 7558
Size: 7564
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3461 5371 5753 6388
Size: 53 83 186 936 1928 1910 382 635
Cuts at: 6388 275
Size: 1451
Fragments arranged by size:
1928 1910 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2119 3054 3840 5997 6395 6669 6816 7227 2119
Size: 935 786 2157 398 274 147 411 2456
Fragments arranged by size:
2456 2157 935 786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2471 2501 2984 3014 3149
Size: 30 165 30 1068 30 483 30 135
Cuts at: 3149 3179 3250 3280 3530 3560 3925 3955 4399
Size: 30 71 30 250 30 365 30 444
Cuts at: 4399 4429 1178
Size: 30 4313
Fragments arranged by size:
4313 1068 483 444 365 250 165 135
71 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2378 2471 2495 2567 2599 2684 2744
Size: 976 216 93 24 72 32 85 60
Cuts at: 2744 2864 3053 3556 3569 3572 4613 4622 1186
Size: 120 189 503 13 3 1041 9 4128
Fragments arranged by size:
4128 1041 976 503 216 189 120 93
85 72 60 32 24 13 9 3
BseYI C'CCAG_C
Cuts at: 2958 3100 4278 6767 7263 2958
Size: 142 1178 2489 496 3259
Fragments arranged by size:
3259 2489 1178 496 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4292 1163
Size: 573 2556 4435
Fragments arranged by size:
4435 2556 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4294 5014 5775 6803 1540
Size: 275 186 2293 720 761 1028 2301
Fragments arranged by size:
2301 2293 1028 761 720 275 186
BsiHKAI G_wGCw'C
Cuts at: 4053 4211 4409 5625 5710 6781 4053
Size: 158 198 1216 85 1071 4836
Fragments arranged by size:
4836 1216 1071 198 158 85
BsmI GAATG_Cn'
Cuts at: 5075 5168 5075
Size: 93 7471
BsmBI CGTCTCn'nnnn_
Cuts at: 2267 3087 3485 4522 2267
Size: 820 398 1037 5309
Fragments arranged by size:
5309 1037 820 398
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2565 3470 3650 4053 4211 4224
Size: 399 254 521 905 180 403 158 13
Cuts at: 4224 4301 4409 4415 4588 4635 4883 5625 5710
Size: 77 108 6 173 47 248 742 85
Cuts at: 5710 6781 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 905 742 521 403 399 254
248 180 173 158 108 85 77 47
13 6
BspEI T'CCGG_A
Cuts at: 2119 3054 3840 7227 2119
Size: 935 786 3387 2456
Fragments arranged by size:
3387 2456 935 786
BspHI T'CATG_A
Cuts at: 5453 7183 5453
Size: 1730 5834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2452 2871 3720 4919 844
Size: 1608 419 849 1199 3489
Fragments arranged by size:
3489 1608 1199 849 419
BsrBI CCG'CTC
Cuts at: 1394 1823 2374 2393 3442 3705 4243 5451 1394
Size: 429 551 19 1049 263 538 1208 3507
Fragments arranged by size:
3507 1208 1049 551 538 429 263 19
BsrDI GCAATG_nn'
Cuts at: 65 1248 6059 6233 65
Size: 1183 4811 174 1396
Fragments arranged by size:
4811 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3674 4126 4289 6208 6395 1700
Size: 110 1864 452 163 1919 187 2869
Fragments arranged by size:
2869 1919 1864 452 187 163 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7564
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7564
BssSI C'ACGA_G
Cuts at: 4223 4408 4532 5624 6636 4223
Size: 185 124 1092 1012 5151
Fragments arranged by size:
5151 1092 1012 185 124
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7564
BstBI TT'CG_AA
Cuts at: 6368 6368
Size: 7564
BstEII G'GTnAC_C
Cuts at: 1184 3826 3905 1184
Size: 2642 79 4843
Fragments arranged by size:
4843 2642 79
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 4129 4669 4774 2011
Size: 2118 540 105 4801
Fragments arranged by size:
4801 2118 540 105
BstYI r'GATC_y
Cuts at: 1972 3251 4778 4894 5261 5646 5663 7115 7201
Size: 1279 1527 116 367 385 17 1452 86
Cuts at: 7201 7213 7559 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1527 1452 1279 385 367 346 116
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4460 4677 4460
Size: 217 7347
BtgI C'CryG_G
Cuts at: 513 1065 2004 2065 3114 4148 4770 513
Size: 552 939 61 1049 1034 622 3307
Fragments arranged by size:
3307 1049 1034 939 622 552 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6825
Fragments arranged by size:
6825 683 56
BtsI GCAGTG_nn'
Cuts at: 2916 5080 5874 5894 2916
Size: 2164 794 20 4586
Fragments arranged by size:
4586 2164 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7564
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7529
DraI TTT'AAA
Cuts at: 4868 4868
Size: 7564
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4510 4514 6571 809
Size: 507 3194 4 2057 1802
Fragments arranged by size:
3194 2057 1802 507 4
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2311 2388 2401
Size: 54 1425 50 275 18 481 77 13
Cuts at: 2401 2814 3300 4131 4291 4560 5011 5900 7529
Size: 413 486 831 160 269 451 889 1629
Cuts at: 7529 8
Size: 43
Fragments arranged by size:
1629 1425 889 831 486 481 451 413
275 269 160 77 54 50 43 18
13
EagI C'GGCC_G
Cuts at: 1537 1812 4291 5011 1537
Size: 275 2479 720 4090
Fragments arranged by size:
4090 2479 720 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 2585 2646 2661 3165 5494 1353
Size: 912 320 61 15 504 2329 3423
Fragments arranged by size:
3423 2329 912 504 320 61 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3602 4338 4765 6151 6525 6671
Size: 1064 138 2013 736 427 1386 374 146
Cuts at: 6671 387
Size: 1280
Fragments arranged by size:
2013 1386 1280 1064 736 427 374 146
138
EcoICRI GAG'CTC
Cuts at: 4051 4051
Size: 7564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2217 2474 2513 2726 3011 3065
Size: 393 533 52 257 39 213 285 54
Cuts at: 3065 3168 3509 3560 3679 3710 3927 4026 4074
Size: 103 341 51 119 31 217 99 48
Cuts at: 4074 5627 6224 7011 7279 1239
Size: 1553 597 787 268 1524
Fragments arranged by size:
1553 1524 787 597 533 393 341 285
268 257 217 213 119 103 99 54
52 51 48 39 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2052 2144 2666 2052
Size: 92 522 6950
Fragments arranged by size:
6950 522 92
EcoO109I rG'GnC_Cy
Cuts at: 4616 4632 4681 4616
Size: 16 49 7499
Fragments arranged by size:
7499 49 16
EcoRI G'AATT_C
Cuts at: 4873 5251 4873
Size: 378 7186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 7564
FseI GG_CCGG'CC
Cuts at: 4293 4293
Size: 7564
FspI TGC'GCA
Cuts at: 2908 3152 3605 6070 2908
Size: 244 453 2465 4402
Fragments arranged by size:
4402 2465 453 244
HaeII r_GCGC'y
Cuts at: 1678 2547 3091 3500 6711 1678
Size: 869 544 409 3211 2531
Fragments arranged by size:
3211 2531 869 544 409
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 2552 2584 3164
Size: 32 135 32 899 32 49 32 580
Cuts at: 3164 3196 3250 3282 4575 4607 4608 4640 4783
Size: 32 54 32 1293 32 1 32 143
Cuts at: 4783 4815 6205 6237 6279 6311 1373
Size: 32 1390 32 42 32 2626
Fragments arranged by size:
2626 1390 1293 899 580 143 135 54
49 42 32 32 32 32 32 32
32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3856 4908 5154 669
Size: 988 281 1918 1052 246 3079
Fragments arranged by size:
3079 1918 1052 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2901 748
Size: 2153 5411
HpaI GTT'AAC
Cuts at: 5154 5154
Size: 7564
KpnI G_GTAC'C
Cuts at: 4676 4889 4676
Size: 213 7351
MfeI C'AATT_G
Cuts at: 5163 5163
Size: 7564
MluI A'CGCG_T
Cuts at: 7440 7440
Size: 7564
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3147 3312 4545 6678 6862 1305
Size: 1842 165 1233 2133 184 2007
Fragments arranged by size:
2133 2007 1842 1233 184 165
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2313 2816 4133 4562 10
Size: 54 1425 343 481 503 1317 429 3012
Fragments arranged by size:
3012 1425 1317 503 481 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 4127 4772 4790 4826 5522 5881
Size: 648 1851 1110 645 18 36 696 359
Cuts at: 5881 6040 518
Size: 159 2042
Fragments arranged by size:
2042 1851 1110 696 648 645 359 159
36 18
MspA1I CmG'CkG
Cuts at: 1606 2155 2704 2746 2962 3324 3609 3742 4980
Size: 549 549 42 216 362 285 133 1238
Cuts at: 4980 5657 6805 7050 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1238 1148 677 549 549 362 285
245 216 133 42
NaeI GCC'GGC
Cuts at: 4291 4291
Size: 7564
NcoI C'CATG_G
Cuts at: 513 1065 2004 3114 4770 513
Size: 552 939 1110 1656 3307
Fragments arranged by size:
3307 1656 1110 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7564
NgoMIV G'CCGG_C
Cuts at: 4289 4289
Size: 7564
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7564
NotI GC'GGCC_GC
Cuts at: 5011 5011
Size: 7564
NspI r_CATG'y
Cuts at: 1132 1229 3691 3991 4789 4922 6467 1132
Size: 97 2462 300 798 133 1545 2229
Fragments arranged by size:
2462 2229 1545 798 300 133 97
PasI CC'CwG_GG
Cuts at: 1348 3643 4216 1348
Size: 2295 573 4696
Fragments arranged by size:
4696 2295 573
PciI A'CATG_T
Cuts at: 6463 6463
Size: 7564
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2938 3100 3487 3583 4097 1108
Size: 1830 162 387 96 514 4575
Fragments arranged by size:
4575 1830 514 387 162 96
PfoI T'CCnGG_A
Cuts at: 3576 3804 3576
Size: 228 7336
PmeI GTTT'AAAC
Cuts at: 4868 4868
Size: 7564
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3530 3550 3562 5633 5665 7146 7178 3518
Size: 12 20 12 2071 32 1481 32 3904
Fragments arranged by size:
3904 2071 1481 32 32 20 12 12
PpuMI rG'GwC_Cy
Cuts at: 4616 4616
Size: 7564
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7564
PsiI TTA'TAA
Cuts at: 2166 5134 2166
Size: 2968 4596
PspOMI G'GGCC_C
Cuts at: 4631 4631
Size: 7564
PstI C_TGCA'G
Cuts at: 830 3165 4731 4916 830
Size: 2335 1566 185 3478
Fragments arranged by size:
3478 2335 1566 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7564
PvuII CAG'CTG
Cuts at: 2155 2704 2746 3324 2155
Size: 549 42 578 6395
Fragments arranged by size:
6395 578 549 42
SacI G_AGCT'C
Cuts at: 4053 4053
Size: 7564
SalI G'TCGA_C
Cuts at: 1936 4906 1936
Size: 2970 4594
SanDI GG'GwC_CC
Cuts at: 4616 4616
Size: 7564
SbfI CC_TGCA'GG
Cuts at: 4916 4916
Size: 7564
ScaI AGT'ACT
Cuts at: 5812 7524 5812
Size: 1712 5852
SexAI A'CCwGG_T
Cuts at: 3234 3234
Size: 7564
SfcI C'TryA_G
Cuts at: 826 1045 3161 4727 4912 6047 6728 6919 826
Size: 219 2116 1566 185 1135 681 191 1471
Fragments arranged by size:
2116 1566 1471 1135 681 219 191 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 4639 4639
Size: 7564
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7564
SgrAI Cr'CCGG_yG
Cuts at: 1700 4126 1700
Size: 2426 5138
SmaI CCC'GGG
Cuts at: 1872 4638 4891 1872
Size: 2766 253 4545
Fragments arranged by size:
4545 2766 253
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 5668 6569 6831 820
Size: 197 926 417 3308 901 262 1553
Fragments arranged by size:
3308 1553 926 901 417 262 197
SnaBI TAC'GTA
Cuts at: 493 5355 493
Size: 4862 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7564
SphI G_CATG'C
Cuts at: 1132 4789 4922 1132
Size: 3657 133 3774
Fragments arranged by size:
3774 3657 133
SspI AAT'ATT
Cuts at: 5 52 5488 5
Size: 47 5436 2081
Fragments arranged by size:
5436 2081 47
StuI AGG'CCT
Cuts at: 1517 4668 1517
Size: 3151 4413
StyI C'CwwG_G
Cuts at: 513 1065 2004 3114 3123 3793 3831 4770 5003
Size: 552 939 1110 9 670 38 939 233
Cuts at: 5003 513
Size: 3074
Fragments arranged by size:
3074 1110 939 939 670 552 233 38
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3637 3969 4387 4520 4820 5591 5608
Size: 1255 2057 332 418 133 300 771 17
Cuts at: 5608 5761 5946 6391 325
Size: 153 185 445 1498
Fragments arranged by size:
2057 1498 1255 771 445 418 332 300
185 153 133 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2680 3870 5810
Size: 274 80 33 51 1443 703 1190 1940
Cuts at: 5810 7522 96
Size: 1712 138
Fragments arranged by size:
1940 1712 1443 1190 703 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2101 2133 4103 4135 4399 4431 7524 7556 2101
Size: 32 1970 32 264 32 3093 32 2109
Fragments arranged by size:
3093 2109 1970 264 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4541 1316
Size: 3225 4339
XbaI T'CTAG_A
Cuts at: 4900 4900
Size: 7564
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3121 3791 3121
Size: 670 6894
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7564
XmaI C'CCGG_G
Cuts at: 1870 4636 4889 1870
Size: 2766 253 4545
Fragments arranged by size:
4545 2766 253
XmnI GAAnn'nnTTC
Cuts at: 5693 5693
Size: 7564
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5372 276
Size: 53 83 186 936 3838 2468
Fragments arranged by size:
3838 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII
EcoRV FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SapI SfoI SrfI SwaI