Restriction map for
pFN21AA1041
(Circular) (Six-base) MAPSORT of: pFN21AA1041.seq Check: 2000 from: 1 to: 6571
pFN21AA1041 6571 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3438 3914 1937
Size: 1501 476 4594
Fragments arranged by size:
4594 1501 476
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3672 4634 6018 6286 1632
Size: 2040 962 1384 268 1917
Fragments arranged by size:
2040 1917 1384 962 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 5470 6447 1203
Size: 4267 977 1327
Fragments arranged by size:
4267 1327 977
AgeI A'CCGG_T
Cuts at: 2761 5402 2761
Size: 2641 3930
AhdI GACnn_n'nnGTC
Cuts at: 5300 5300
Size: 6571
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6571
AlwNI CAG_nnn'CTG
Cuts at: 4314 5886 4314
Size: 1572 4999
ApaLI G'TGCA_C
Cuts at: 4628 5784 4628
Size: 1156 5415
ApoI r'AATT_y
Cuts at: 1493 2160 2313 2374 3652 3880 4106 4258 6367
Size: 667 153 61 1278 228 226 152 2109
Cuts at: 6367 1493
Size: 1697
Fragments arranged by size:
2109 1697 1278 667 228 226 153 152
61
AseI AT'TA_AT
Cuts at: 160 1032 2277 5125 160
Size: 872 1245 2848 1606
Fragments arranged by size:
2848 1606 1245 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 3896 1870
Size: 73 1953 4545
Fragments arranged by size:
4545 1953 73
BamHI G'GATC_C
Cuts at: 3901 4268 3901
Size: 367 6204
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3319 3608 3892 5347 618
Size: 325 217 627 1532 289 284 1455 1842
Fragments arranged by size:
1842 1532 1455 627 325 289 284 217
BanII G_rGCy'C
Cuts at: 1391 3890 1391
Size: 2499 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5600
BbvCI CC'TCA_GC
Cuts at: 2805 2805
Size: 6571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4762 4796 1903
Size: 34 2825 34 3678
Fragments arranged by size:
3678 2825 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3352 4463 5673 3352
Size: 1111 1210 4250
Fragments arranged by size:
4250 1210 1111
BclI T'GATC_A
Cuts at: 1548 2653 1548
Size: 1105 5466
BfrBI ATG'CAT
Cuts at: 3616 3616
Size: 6571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2500 5182 136
Size: 107 122 71 631 605 828 2682 1525
Fragments arranged by size:
2682 1525 828 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2875 3484 6566 2875
Size: 609 3082 2880
Fragments arranged by size:
3082 2880 609
BlpI GC'TnA_GC
Cuts at: 3988 3988
Size: 6571
Bme1580I G_kGCm'C
Cuts at: 1790 2518 4632 5788 1790
Size: 728 2114 1156 2573
Fragments arranged by size:
2573 2114 1156 728
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6571
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2467 4302 5260
Size: 416 53 83 399 24 1050 1835 958
Cuts at: 5260 442
Size: 1753
Fragments arranged by size:
1835 1753 1050 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 3218 3723 3813 5231 1239
Size: 845 1134 505 90 1418 2579
Fragments arranged by size:
2579 1418 1134 845 505 90
Bpu10I CC'TnA_GC
Cuts at: 2805 2805
Size: 6571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3473 4696 5561 5859 3473
Size: 1223 865 298 4185
Fragments arranged by size:
4185 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3112 5234 882
Size: 617 1613 2122 2219
Fragments arranged by size:
2219 2122 1613 617
BsaAI yAC'GTr
Cuts at: 493 1204 4362 493
Size: 711 3158 2702
Fragments arranged by size:
3158 2702 711
BsaBI GATnn'nnATC
Cuts at: 2948 6565 2948
Size: 3617 2954
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4378 4760 5395 275
Size: 53 83 186 936 2845 382 635 1451
Fragments arranged by size:
2845 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2761 5004 5402 5676 5823 6234 2761
Size: 2243 398 274 147 411 3098
Fragments arranged by size:
3098 2243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6346
Fragments arranged by size:
6346 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2793 1186
Size: 1607 4964
BseYI C'CCAG_C
Cuts at: 2967 3005 3202 3249 5774 6270 2967
Size: 38 197 47 2525 496 3268
Fragments arranged by size:
3268 2525 496 197 47 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5998
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3164 4021 4782 5810 1540
Size: 275 186 1163 857 761 1028 2301
Fragments arranged by size:
2301 1163 1028 857 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3023 4632 4717 5788 3023
Size: 1609 85 1071 3806
Fragments arranged by size:
3806 1609 1071 85
BsmI GAATG_Cn'
Cuts at: 2179 4082 4175 2179
Size: 1903 93 4575
Fragments arranged by size:
4575 1903 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2518 3023 3890 4632 4717 5788 1391
Size: 399 728 505 867 742 85 1071 2174
Fragments arranged by size:
2174 1071 867 742 728 505 399 85
BspEI T'CCGG_A
Cuts at: 6234 6234
Size: 6571
BspHI T'CATG_A
Cuts at: 4460 6190 4460
Size: 1730 4841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3926 844
Size: 3082 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4458 1394
Size: 429 2635 3507
Fragments arranged by size:
3507 2635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5066 5240 65
Size: 1183 3818 174 1396
Fragments arranged by size:
3818 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2761 5215 5402 1700
Size: 110 951 2454 187 2869
Fragments arranged by size:
2869 2454 951 187 110
BsrGI T'GTAC_A
Cuts at: 96 3657 96
Size: 3561 3010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 4631 5643 4631
Size: 1012 5559
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3790 794
Size: 2996 3575
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 2091 1184
Size: 907 5664
BstXI CCAn_nnnn'nTGG
Cuts at: 3641 3641
Size: 6571
BstYI r'GATC_y
Cuts at: 1972 2875 3484 3901 4268 4653 4670 6122 6208
Size: 903 609 417 367 385 17 1452 86
Cuts at: 6208 6220 6566 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 903 609 417 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5080
Fragments arranged by size:
5080 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5832
Fragments arranged by size:
5832 683 56
BtsI GCAGTG_nn'
Cuts at: 2368 3073 3424 4087 4881 4901 2368
Size: 705 351 663 794 20 4038
Fragments arranged by size:
4038 794 705 663 351 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6536
DraI TTT'AAA
Cuts at: 3875 3875
Size: 6571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2025 5578 809
Size: 507 709 3553 1802
Fragments arranged by size:
3553 1802 709 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2501 4018 4907
Size: 54 1425 50 275 18 671 1517 889
Cuts at: 4907 6536 8
Size: 1629 43
Fragments arranged by size:
1629 1517 1425 889 671 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4018 1537
Size: 275 2206 4090
Fragments arranged by size:
4090 2206 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2504 4501 1353
Size: 1151 1997 3423
Fragments arranged by size:
3423 1997 1151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5158 5532 5678 387
Size: 1064 138 3569 374 146 1280
Fragments arranged by size:
3569 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 3218 3672 3723 3813 4634 5231
Size: 393 452 1134 454 51 90 821 597
Cuts at: 5231 6018 6286 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1134 821 787 597 454 452 393
268 90 51
EcoO109I rG'GnC_Cy
Cuts at: 2451 2451
Size: 6571
EcoRI G'AATT_C
Cuts at: 2374 3880 4258 2374
Size: 1506 378 4687
Fragments arranged by size:
4687 1506 378
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 5718 1678
Size: 4040 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2103 2135 2758 2790 5212
Size: 32 135 32 531 32 623 32 2422
Cuts at: 5212 5244 5286 5318 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2422 623 531 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2933 3309 3915 4161 669
Size: 988 281 995 376 606 246 3079
Fragments arranged by size:
3079 995 988 606 376 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6571
HpaI GTT'AAC
Cuts at: 3309 4161 3309
Size: 852 5719
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 3864 4170 3864
Size: 306 6265
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2242 2348 2627 2719 3078 3644 5685 5869
Size: 937 106 279 92 359 566 2041 184
Cuts at: 5869 1305
Size: 2007
Fragments arranged by size:
2041 2007 937 566 359 279 184 106
92
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4749
Fragments arranged by size:
4749 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2229 3066 4529 4888 5047 518
Size: 648 1063 837 1463 359 159 2042
Fragments arranged by size:
2042 1463 1063 837 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3987 4664 5812 6057 1606
Size: 2381 677 1148 245 2120
Fragments arranged by size:
2381 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5080
Fragments arranged by size:
5080 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6571
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NsiI A_TGCA'T
Cuts at: 3618 3618
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 3929 5474 1132
Size: 97 2700 1545 2229
Fragments arranged by size:
2700 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6571
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2713 3704 1108
Size: 1605 991 3975
Fragments arranged by size:
3975 1605 991
PfoI T'CCnGG_A
Cuts at: 3739 3840 3739
Size: 101 6470
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6153 6185 4640
Size: 32 1481 32 5026
Fragments arranged by size:
5026 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2451 2451
Size: 6571
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 2904 4141 2904
Size: 1237 5334
PstI C_TGCA'G
Cuts at: 830 3923 830
Size: 3093 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SbfI CC_TGCA'GG
Cuts at: 3923 3923
Size: 6571
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SexAI A'CCwGG_T
Cuts at: 3507 3507
Size: 6571
SfcI C'TryA_G
Cuts at: 826 1045 2523 3919 5054 5735 5926 826
Size: 219 1478 1396 1135 681 191 1471
Fragments arranged by size:
1478 1471 1396 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 2761 1700
Size: 1061 5510
SmaI CCC'GGG
Cuts at: 1872 3898 1872
Size: 2026 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3488 4675 5576 5838 820
Size: 197 926 1545 1187 901 262 1553
Fragments arranged by size:
1553 1545 1187 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3929 1132
Size: 2797 3774
SspI AAT'ATT
Cuts at: 5 52 4495 5
Size: 47 4443 2081
Fragments arranged by size:
4443 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6571
StyI C'CwwG_G
Cuts at: 513 1065 2004 4010 513
Size: 552 939 2006 3074
Fragments arranged by size:
3074 2006 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2302 3042 3147 3216 3228 4598 4615
Size: 1255 722 740 105 69 12 1370 17
Cuts at: 4615 4768 4953 5398 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1370 1255 740 722 445 185 153
105 69 17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2820 3011 3657
Size: 274 80 33 51 1443 843 191 646
Cuts at: 3657 4817 6529 96
Size: 1160 1712 138
Fragments arranged by size:
1712 1443 1160 843 646 274 191 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6531 6563 6531
Size: 32 6539
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6571
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3164 3164
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6571
XmaI C'CCGG_G
Cuts at: 1870 3896 1870
Size: 2026 4545
XmnI GAAnn'nnTTC
Cuts at: 4700 4700
Size: 6571
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI