Restriction map for
pFN21AA1032
(Circular) (Six-base) MAPSORT of: pFN21AA1032.seq Check: 3130 from: 1 to: 9814
pFN21AA1032 9814 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6847 6847
Size: 9814
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7624 278
Size: 53 83 186 936 6088 2468
Fragments arranged by size:
6088 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7157 1937
Size: 5220 4594
Acc65I G'GTAC_C
Cuts at: 7135 7135
Size: 9814
AclI AA'CG_TT
Cuts at: 1563 7941 8314 1563
Size: 6378 373 3063
Fragments arranged by size:
6378 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2427 3039 5277 5855 5865 5882 6137 6149
Size: 795 612 2238 578 10 17 255 12
Cuts at: 6149 7877 9261 9529 1632
Size: 1728 1384 268 1917
Fragments arranged by size:
2238 1917 1728 1384 795 612 578 268
255 17 12 10
AfeI AGC'GCT
Cuts at: 4799 5254 4799
Size: 455 9359
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9617
AflIII A'CryG_T
Cuts at: 1203 2067 4344 4752 8713 9690 1203
Size: 864 2277 408 3961 977 1327
Fragments arranged by size:
3961 2277 1327 977 864 408
AgeI A'CCGG_T
Cuts at: 8645 8645
Size: 9814
AhdI GACnn_n'nnGTC
Cuts at: 3334 4015 5146 8543 3334
Size: 681 1131 3397 4605
Fragments arranged by size:
4605 3397 1131 681
AleI CACnn'nnGTG
Cuts at: 1166 2072 2225 4410 4751 6429 1166
Size: 906 153 2185 341 1678 4551
Fragments arranged by size:
4551 2185 1678 906 341 153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2466 3840 4238 1184
Size: 1282 1374 398 6760
Fragments arranged by size:
6760 1374 1282 398
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 5795 5827 2138
Size: 32 3625 32 6125
Fragments arranged by size:
6125 3625 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3796 5233 5461 6012 6629 7557 9129 3796
Size: 1437 228 551 617 928 1572 4481
Fragments arranged by size:
4481 1572 1437 928 617 551 228
ApaI G_GGCC'C
Cuts at: 2282 5124 6732 2282
Size: 2842 1608 5364
Fragments arranged by size:
5364 2842 1608
ApaLI G'TGCA_C
Cuts at: 3652 7871 9027 3652
Size: 4219 1156 4439
Fragments arranged by size:
4439 4219 1156
ApoI r'AATT_y
Cuts at: 1493 2060 4180 5194 7123 7349 7501 9610 1493
Size: 567 2120 1014 1929 226 152 2109 1697
Fragments arranged by size:
2120 2109 1929 1697 1014 567 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8368 160
Size: 872 7336 1606
Fragments arranged by size:
7336 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9814
AvaI C'yCGr_G
Cuts at: 1870 1943 2709 3399 3920 4974 5484 5621 6839
Size: 73 766 690 521 1054 510 137 1218
Cuts at: 6839 7139 1870
Size: 300 4545
Fragments arranged by size:
4545 1218 1054 766 690 521 510 300
137 73
BamHI G'GATC_C
Cuts at: 2241 4088 7144 7511 2241
Size: 1847 3056 367 4544
Fragments arranged by size:
4544 3056 1847 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2339 3055 3175 3235
Size: 325 217 627 261 291 716 120 60
Cuts at: 3235 4391 4681 5501 7097 7135 8590 618
Size: 1156 290 820 1596 38 1455 1842
Fragments arranged by size:
1842 1596 1455 1156 820 716 627 325
291 290 261 217 120 60 38
BanII G_rGCy'C
Cuts at: 1391 2282 2708 2873 3111 3839 3936 4970 5124
Size: 891 426 165 238 728 97 1034 154
Cuts at: 5124 5904 6470 6732 6790 7133 1391
Size: 780 566 262 58 343 4072
Fragments arranged by size:
4072 1034 891 780 728 566 426 343
262 238 165 154 97 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3096 3626 4130 5193 5572 6113 6455
Size: 971 1197 530 504 1063 379 541 342
Cuts at: 6455 6582 928
Size: 127 4160
Fragments arranged by size:
4160 1197 1063 971 541 530 504 379
342 127
BbvCI CC'TCA_GC
Cuts at: 2768 2768
Size: 9814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2092 2126 4687 4721 8005 8039 1903
Size: 34 155 34 2561 34 3284 34 3678
Fragments arranged by size:
3678 3284 2561 155 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7706 8916 7706
Size: 1210 8604
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9814
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2868 3173 3206
Size: 107 122 71 631 605 1196 305 33
Cuts at: 3206 3450 8425 136
Size: 244 4975 1525
Fragments arranged by size:
4975 1525 1196 631 605 305 244 122
107 71 33
BglII A'GATC_T
Cuts at: 2331 3924 3945 9809 2331
Size: 1593 21 5864 2336
Fragments arranged by size:
5864 2336 1593 21
BlpI GC'TnA_GC
Cuts at: 2810 2831 3105 3246 3264 4328 5903 6737 6821
Size: 21 274 141 18 1064 1575 834 84
Cuts at: 6821 7231 2810
Size: 410 5393
Fragments arranged by size:
5393 1575 1064 834 410 274 141 84
21 18
Bme1580I G_kGCm'C
Cuts at: 1790 2053 2234 2282 3656 4676 5124 5506 5652
Size: 263 181 48 1374 1020 448 382 146
Cuts at: 5652 5760 5990 6732 7875 9031 1790
Size: 108 230 742 1143 1156 2573
Fragments arranged by size:
2573 1374 1156 1143 1020 742 448 382
263 230 181 146 108 48
BmgBI CAC'GTC
Cuts at: 1318 4017 4345 4501 1318
Size: 2699 328 156 6631
Fragments arranged by size:
6631 2699 328 156
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2434 3164 6639
Size: 416 53 83 399 24 1017 730 3475
Cuts at: 6639 7545 8503 442
Size: 906 958 1753
Fragments arranged by size:
3475 1753 1017 958 906 730 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2767 3853 2767
Size: 1086 8728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2304 2459 2996 3008 3020 3056 3559 4877
Size: 1065 155 537 12 12 36 503 1318
Cuts at: 4877 4946 5528 5678 5822 6479 8474 1239
Size: 69 582 150 144 657 1995 2579
Fragments arranged by size:
2579 1995 1318 1065 657 582 537 503
155 150 144 69 36 12 12
Bpu10I CC'TnA_GC
Cuts at: 2768 5972 2768
Size: 3204 6610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3869 6238 6314 6428 6659 7049 7939 8804 9102
Size: 2369 76 114 231 390 890 865 298
Cuts at: 9102 3869
Size: 4581
Fragments arranged by size:
4581 2369 890 865 390 298 231 114
76
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2213 3461 8477 882
Size: 617 714 1248 5016 2219
Fragments arranged by size:
5016 2219 1248 714 617
BsaAI yAC'GTr
Cuts at: 493 1204 2070 2074 7605 493
Size: 711 866 4 5531 2702
Fragments arranged by size:
5531 2702 866 711 4
BsaBI GATnn'nnATC
Cuts at: 4040 9808 4040
Size: 5768 4046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3659 4614 7621 8003
Size: 53 83 186 936 2126 955 3007 382
Cuts at: 8003 8638 275
Size: 635 1451
Fragments arranged by size:
3007 2126 1451 955 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3858 4857 8247 8645 8919 9066 9477 3858
Size: 999 3390 398 274 147 411 4195
Fragments arranged by size:
4195 3390 999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2711 2741 2885 2915 4190
Size: 30 165 30 1308 30 144 30 1275
Cuts at: 4190 4220 4944 4974 5696 5711 5726 5741 5795
Size: 30 724 30 722 15 15 15 54
Cuts at: 5795 5825 7064 7094 1178
Size: 30 1239 30 3898
Fragments arranged by size:
3898 1308 1275 1239 724 722 165 144
54 30 30 30 30 30 30 30
30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2426 2759 2993 3017 3020 3023 3041
Size: 1102 138 333 234 24 3 3 18
Cuts at: 3041 3044 3053 3056 3059 3062 3065 3296 3608
Size: 3 9 3 3 3 3 231 312
Cuts at: 3608 4190 4445 4648 5318 5489 5681 7103 1186
Size: 582 255 203 670 171 192 1422 3897
Fragments arranged by size:
3897 1422 1102 670 582 333 312 255
234 231 203 192 171 138 24 18
9 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2132 2136 2513 2547 2952 3081 4870 5123 6310
Size: 4 377 34 405 129 1789 253 1187
Cuts at: 6310 6732 6783 6936 9017 9513 2132
Size: 422 51 153 2081 496 2433
Fragments arranged by size:
2433 2081 1789 1187 496 422 405 377
253 153 129 51 34 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 2601 3739 4523 5480 1163
Size: 573 372 493 1138 784 957 5497
Fragments arranged by size:
5497 1138 957 784 573 493 372
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4213 4768 6704 6973 7264 8025
Size: 275 186 2212 555 1936 269 291 761
Cuts at: 8025 9053 1540
Size: 1028 2301
Fragments arranged by size:
2301 2212 1936 1028 761 555 291 275
269 186
BsiHKAI G_wGCw'C
Cuts at: 2105 2859 3656 3839 3885 3936 4700 5310 5691
Size: 754 797 183 46 51 764 610 381
Cuts at: 5691 5904 7875 7960 9031 2105
Size: 213 1971 85 1071 2888
Fragments arranged by size:
2888 1971 1071 797 764 754 610 381
213 183 85 51 46
BsiWI C'GTAC_G
Cuts at: 2070 2070
Size: 9814
BsmI GAATG_Cn'
Cuts at: 5763 7325 7418 5763
Size: 1562 93 8159
Fragments arranged by size:
8159 1562 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2105 2234 2282 2708 2859 2873
Size: 399 263 52 129 48 426 151 14
Cuts at: 2873 3111 3656 3839 3885 3936 4676 4700 4970
Size: 238 545 183 46 51 740 24 270
Cuts at: 4970 5124 5310 5506 5652 5691 5760 5904 5990
Size: 154 186 196 146 39 69 144 86
Cuts at: 5990 6470 6732 6790 7133 7875 7960 9031 1391
Size: 480 262 58 343 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 740 545 480 426 399
343 270 263 262 238 196 186 183
154 151 146 144 129 86 85 69
58 52 51 48 46 39 24 14
BspEI T'CCGG_A
Cuts at: 9477 9477
Size: 9814
BspHI T'CATG_A
Cuts at: 4692 5769 7703 9433 4692
Size: 1077 1934 1730 5073
Fragments arranged by size:
5073 1934 1730 1077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3804 6072 6483 6531 6847 7169 844
Size: 2960 2268 411 48 316 322 3489
Fragments arranged by size:
3489 2960 2268 411 322 316 48
BsrBI CCG'CTC
Cuts at: 1394 1823 2890 6967 7701 1394
Size: 429 1067 4077 734 3507
Fragments arranged by size:
4077 3507 1067 734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6001 8309 8483 65
Size: 1183 4753 2308 174 1396
Fragments arranged by size:
4753 2308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8458 8645 1700
Size: 110 6648 187 2869
Fragments arranged by size:
6648 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5058 6541 96
Size: 4962 1483 3369
Fragments arranged by size:
4962 3369 1483
BssHII G'CGCG_C
Cuts at: 1925 6884 1925
Size: 4959 4855
BssSI C'ACGA_G
Cuts at: 3509 3649 3779 7874 8886 3509
Size: 140 130 4095 1012 4437
Fragments arranged by size:
4437 4095 1012 140 130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2523 2863 3748 3753 3762 4003 6484 794
Size: 1729 340 885 5 9 241 2481 4124
Fragments arranged by size:
4124 2481 1729 885 340 241 9 5
BstBI TT'CG_AA
Cuts at: 3672 5814 8618 3672
Size: 2142 2804 4868
Fragments arranged by size:
4868 2804 2142
BstEII G'GTnAC_C
Cuts at: 1184 3955 1184
Size: 2771 7043
BstXI CCAn_nnnn'nTGG
Cuts at: 2437 3532 4987 6310 6431 2437
Size: 1095 1455 1323 121 5820
Fragments arranged by size:
5820 1455 1323 1095 121
BstYI r'GATC_y
Cuts at: 1972 2241 2331 3252 3900 3924 3945 4088 4524
Size: 269 90 921 648 24 21 143 436
Cuts at: 4524 7144 7511 7896 7913 9365 9451 9463 9809
Size: 2620 367 385 17 1452 86 12 346
Cuts at: 9809 1972
Size: 1977
Fragments arranged by size:
2620 1977 1452 921 648 436 385 367
346 269 143 90 86 24 21 17
12
Bsu36I CC'TnA_GG
Cuts at: 3258 3258
Size: 9814
BtgI C'CryG_G
Cuts at: 513 1065 2775 4047 5298 5568 5820 6159 513
Size: 552 1710 1272 1251 270 252 339 4168
Fragments arranged by size:
4168 1710 1272 1251 552 339 270 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2095 3718 487
Size: 683 56 869 1623 6583
Fragments arranged by size:
6583 1623 869 683 56
BtsI GCAGTG_nn'
Cuts at: 2589 2617 6383 7330 8124 8144 2589
Size: 28 3766 947 794 20 4259
Fragments arranged by size:
4259 3766 947 794 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9814
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9779
DraI TTT'AAA
Cuts at: 7118 7118
Size: 9814
DraIII CAC_nnn'GTG
Cuts at: 2238 3762 4492 2238
Size: 1524 730 7560
Fragments arranged by size:
7560 1524 730
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2309 4103 8821 809
Size: 507 993 1794 4718 1802
Fragments arranged by size:
4718 1802 1794 993 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3198 3522 3684
Size: 54 1425 50 275 18 1368 324 162
Cuts at: 3684 5085 6701 6909 6970 7044 7261 8150 9779
Size: 1401 1616 208 61 74 217 889 1629
Cuts at: 9779 8
Size: 43
Fragments arranged by size:
1629 1616 1425 1401 1368 889 324 275
217 208 162 74 61 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6701 6970 7261 1537
Size: 275 4889 269 291 4090
Fragments arranged by size:
4889 4090 291 275 269
EarI CTCTTCn'nnn_
Cuts at: 1353 2403 2803 2964 3827 5937 6458 7744 1353
Size: 1050 400 161 863 2110 521 1286 3423
Fragments arranged by size:
3423 2110 1286 1050 863 521 400 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 3866 4018 7072 8401 8775
Size: 1064 138 660 1617 152 3054 1329 374
Cuts at: 8775 8921 387
Size: 146 1280
Fragments arranged by size:
3054 1617 1329 1280 1064 660 374 152
146 138
EcoICRI GAG'CTC
Cuts at: 3837 3934 5902 3837
Size: 97 1968 7749
Fragments arranged by size:
7749 1968 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2304 2427 2459 2996 3008 3020 3039
Size: 393 672 123 32 537 12 12 19
Cuts at: 3039 3056 3559 4877 4946 5277 5528 5678 5822
Size: 17 503 1318 69 331 251 150 144
Cuts at: 5822 5855 5865 5882 6137 6149 6479 7877 8474
Size: 33 10 17 255 12 330 1398 597
Cuts at: 8474 9261 9529 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1398 1318 787 672 597 537 503
393 331 330 268 255 251 150 144
123 69 33 32 19 17 17 12
12 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 3002 5975 3002
Size: 2973 6841
EcoO109I rG'GnC_Cy
Cuts at: 2278 2279 2347 2983 3166 3187 3793 4897 5120
Size: 1 68 636 183 21 606 1104 223
Cuts at: 5120 6729 6836 2278
Size: 1609 107 5256
Fragments arranged by size:
5256 1609 1104 636 606 223 183 107
68 21 1
EcoRI G'AATT_C
Cuts at: 5194 7123 7501 5194
Size: 1929 378 7507
Fragments arranged by size:
7507 1929 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5030 6469 5030
Size: 1439 8375
FspI TGC'GCA
Cuts at: 8320 8320
Size: 9814
HaeII r_GCGC'y
Cuts at: 1678 4801 5256 5665 6828 6951 8961 1678
Size: 3123 455 409 1163 123 2010 2531
Fragments arranged by size:
3123 2531 2010 1163 455 409 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2152 2184 2885 2917 3320
Size: 32 135 32 580 32 701 32 403
Cuts at: 3320 3352 4001 4033 4238 4270 4889 4921 5132
Size: 32 649 32 205 32 619 32 211
Cuts at: 5132 5164 5254 5286 5465 5497 5567 5599 5696
Size: 32 90 32 179 32 70 32 97
Cuts at: 5696 5711 5728 5743 6464 6496 7064 7096 8455
Size: 15 17 15 721 32 568 32 1359
Cuts at: 8455 8487 8529 8561 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1359 721 701 649 619 580 568
403 211 205 179 135 97 90 70
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 6718 7158 7404 669
Size: 988 281 4780 440 246 3079
Fragments arranged by size:
4780 3079 988 440 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9814
HpaI GTT'AAC
Cuts at: 7404 7404
Size: 9814
KpnI G_GTAC'C
Cuts at: 7139 7139
Size: 9814
MfeI C'AATT_G
Cuts at: 7413 7413
Size: 9814
MluI A'CGCG_T
Cuts at: 9690 9690
Size: 9814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2002 2898 4233 4788 4806 7050 8928 9112
Size: 697 896 1335 555 18 2244 1878 184
Cuts at: 9112 1305
Size: 2007
Fragments arranged by size:
2244 2007 1878 1335 896 697 555 184
18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3200 3524 7046 10
Size: 54 1425 343 1368 324 3522 2778
Fragments arranged by size:
3522 2778 1425 1368 343 324 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2114 2225 2565 3875 3878 4046
Size: 648 906 42 111 340 1310 3 168
Cuts at: 4046 4220 4316 4410 4412 4751 5768 6429 6983
Size: 174 96 94 2 339 1017 661 554
Cuts at: 6983 7772 8131 8290 518
Size: 789 359 159 2042
Fragments arranged by size:
2042 1310 1017 906 789 661 648 554
359 340 339 174 168 159 111 96
94 42 3 2
MspA1I CmG'CkG
Cuts at: 1606 2136 2830 3143 3245 3388 3820 4332 4822
Size: 530 694 313 102 143 432 512 490
Cuts at: 4822 5800 6319 6922 7230 7907 9055 9300 1606
Size: 978 519 603 308 677 1148 245 2120
Fragments arranged by size:
2120 1148 978 694 677 603 530 519
512 490 432 313 308 245 143 102
NcoI C'CATG_G
Cuts at: 513 1065 5298 5820 6159 513
Size: 552 4233 522 339 4168
Fragments arranged by size:
4233 4168 552 522 339
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9814
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9814
NotI GC'GGCC_GC
Cuts at: 7261 7261
Size: 9814
NruI TCG'CGA
Cuts at: 3737 3737
Size: 9814
NspI r_CATG'y
Cuts at: 1132 1229 3478 3751 5552 7172 8717 1132
Size: 97 2249 273 1801 1620 1545 2229
Fragments arranged by size:
2249 2229 1801 1620 1545 273 97
PasI CC'CwG_GG
Cuts at: 1348 2701 3149 3446 4060 4676 6559 1348
Size: 1353 448 297 614 616 1883 4603
Fragments arranged by size:
4603 1883 1353 616 614 448 297
PciI A'CATG_T
Cuts at: 8713 8713
Size: 9814
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5461 6565 6629 1108
Size: 4353 1104 64 4293
Fragments arranged by size:
4353 4293 1104 64
PfoI T'CCnGG_A
Cuts at: 2370 2997 3542 3903 3939 4812 4893 6456 6622
Size: 627 545 361 36 873 81 1563 166
Cuts at: 6622 2370
Size: 5562
Fragments arranged by size:
5562 1563 873 627 545 361 166 81
36
PmeI GTTT'AAAC
Cuts at: 7118 7118
Size: 9814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4466 4498 5795 5827 7883 7915 9396 9428 4466
Size: 32 1297 32 2056 32 1481 32 4852
Fragments arranged by size:
4852 2056 1481 1297 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2347 2983 3793 4897 6836 2347
Size: 636 810 1104 1939 5325
Fragments arranged by size:
5325 1939 1104 810 636
PshAI GACnn'nnGTC
Cuts at: 1613 6834 1613
Size: 5221 4593
PsiI TTA'TAA
Cuts at: 7384 7384
Size: 9814
PspOMI G'GGCC_C
Cuts at: 2278 5120 6728 2278
Size: 2842 1608 5364
Fragments arranged by size:
5364 2842 1608
PspXI vC'TCGA_Gb
Cuts at: 5621 5621
Size: 9814
PstI C_TGCA'G
Cuts at: 830 2868 3244 3393 3819 6017 6858 6921 7166
Size: 2038 376 149 426 2198 841 63 245
Cuts at: 7166 830
Size: 3478
Fragments arranged by size:
3478 2198 2038 841 426 376 245 149
63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9814
PvuII CAG'CTG
Cuts at: 2136 2830 3143 3245 3388 4822 5800 6319 6922
Size: 694 313 102 143 1434 978 519 603
Cuts at: 6922 2136
Size: 5028
Fragments arranged by size:
5028 1434 978 694 603 519 313 143
102
SacI G_AGCT'C
Cuts at: 3839 3936 5904 3839
Size: 97 1968 7749
Fragments arranged by size:
7749 1968 97
SalI G'TCGA_C
Cuts at: 1936 7156 1936
Size: 5220 4594
SanDI GG'GwC_CC
Cuts at: 6836 6836
Size: 9814
SapI GCTCTTCn'nnn_
Cuts at: 2964 3827 6458 2964
Size: 863 2631 6320
Fragments arranged by size:
6320 2631 863
SbfI CC_TGCA'GG
Cuts at: 2868 7166 2868
Size: 4298 5516
ScaI AGT'ACT
Cuts at: 8062 9774 8062
Size: 1712 8102
SfcI C'TryA_G
Cuts at: 826 1045 2719 2864 3240 3389 3815 6013 6854
Size: 219 1674 145 376 149 426 2198 841
Cuts at: 6854 6917 7162 8297 8978 9169 826
Size: 63 245 1135 681 191 1471
Fragments arranged by size:
2198 1674 1471 1135 841 681 426 376
245 219 191 149 145 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 3206 3206
Size: 9814
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9814
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9814
SmaI CCC'GGG
Cuts at: 1872 2711 3401 5486 7141 1872
Size: 839 690 2085 1655 4545
Fragments arranged by size:
4545 2085 1655 839 690
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3884 5621 6217 6329 6443 6674
Size: 197 926 1941 1737 596 112 114 231
Cuts at: 6674 7064 7918 8819 9081 820
Size: 390 854 901 262 1553
Fragments arranged by size:
1941 1737 1553 926 901 854 596 390
262 231 197 114 112
SnaBI TAC'GTA
Cuts at: 493 7605 493
Size: 7112 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9814
SphI G_CATG'C
Cuts at: 1132 3478 3751 5552 7172 1132
Size: 2346 273 1801 1620 3774
Fragments arranged by size:
3774 2346 1801 1620 273
SspI AAT'ATT
Cuts at: 5 52 2474 2681 6419 7738 5
Size: 47 2422 207 3738 1319 2081
Fragments arranged by size:
3738 2422 2081 1319 207 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9814
StyI C'CwwG_G
Cuts at: 513 1065 2343 3201 3273 3345 3567 4536 5298
Size: 552 1278 858 72 72 222 969 762
Cuts at: 5298 5715 5739 5820 6159 6303 7047 7253 513
Size: 417 24 81 339 144 744 206 3074
Fragments arranged by size:
3074 1278 969 858 762 744 552 417
339 222 206 144 81 72 72 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2914 3193 3286 4511 5951 6557 6575
Size: 1255 1334 279 93 1225 1440 606 18
Cuts at: 6575 7841 7858 8011 8196 8641 325
Size: 1266 17 153 185 445 1498
Fragments arranged by size:
1498 1440 1334 1266 1255 1225 606 445
279 185 153 93 18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4458 5031 5058
Size: 274 80 33 51 1443 2481 573 27
Cuts at: 5058 6541 6795 8060 9772 96
Size: 1483 254 1265 1712 138
Fragments arranged by size:
2481 1712 1483 1443 1265 573 274 254
138 80 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2172 2204 4190 4222 9774 9806 2172
Size: 32 1986 32 5552 32 2180
Fragments arranged by size:
5552 2180 1986 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9814
XbaI T'CTAG_A
Cuts at: 7150 7150
Size: 9814
XhoI C'TCGA_G
Cuts at: 1943 5621 1943
Size: 3678 6136
XmaI C'CCGG_G
Cuts at: 1870 2709 3399 5484 7139 1870
Size: 839 690 2085 1655 4545
Fragments arranged by size:
4545 2085 1655 839 690
XmnI GAAnn'nnTTC
Cuts at: 4481 4865 5810 6452 7943 4481
Size: 384 945 642 1491 6352
Fragments arranged by size:
6352 1491 945 642 384
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7622 276
Size: 53 83 186 936 6088 2468
Fragments arranged by size:
6088 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsmBI BstZ17I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI
PmlI PsrI RsrII SacII SexAI SfoI SrfI SwaI
XcmI