Restriction map for
pFN21AA1031
(Circular) (Six-base) MAPSORT of: pFN21AA1031.seq Check: 8552 from: 1 to: 7636
pFN21AA1031 7636 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4108 4108
Size: 7636
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5446 278
Size: 53 83 186 936 3910 2468
Fragments arranged by size:
3910 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2060 3104 4979 1937
Size: 123 1044 1875 4594
Fragments arranged by size:
4594 1875 1044 123
Acc65I G'GTAC_C
Cuts at: 4957 4957
Size: 7636
AclI AA'CG_TT
Cuts at: 1563 5763 6136 1563
Size: 4200 373 3063
Fragments arranged by size:
4200 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3653 4802 5699 7083 7351 1632
Size: 2021 1149 897 1384 268 1917
Fragments arranged by size:
2021 1917 1384 1149 897 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7439
AflIII A'CryG_T
Cuts at: 1203 3534 3849 4359 4413 6535 7512 1203
Size: 2331 315 510 54 2122 977 1327
Fragments arranged by size:
2331 2122 1327 977 510 315 54
AgeI A'CCGG_T
Cuts at: 6467 6467
Size: 7636
AhdI GACnn_n'nnGTC
Cuts at: 2818 3721 6365 2818
Size: 903 2644 4089
Fragments arranged by size:
4089 2644 903
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7636
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2870 4829 1184
Size: 1686 1959 3991
Fragments arranged by size:
3991 1959 1686
AlwNI CAG_nnn'CTG
Cuts at: 2119 2170 2491 4641 5379 6951 2119
Size: 51 321 2150 738 1572 2804
Fragments arranged by size:
2804 2150 1572 738 321 51
ApaI G_GGCC'C
Cuts at: 3035 4062 3035
Size: 1027 6609
ApaLI G'TGCA_C
Cuts at: 3779 5693 6849 3779
Size: 1914 1156 4566
Fragments arranged by size:
4566 1914 1156
ApoI r'AATT_y
Cuts at: 1493 4945 5171 5323 7432 1493
Size: 3452 226 152 2109 1697
Fragments arranged by size:
3452 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6190 160
Size: 872 5158 1606
Fragments arranged by size:
5158 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7636
AvaI C'yCGr_G
Cuts at: 1870 1943 2235 2646 3028 3306 4470 4961 1870
Size: 73 292 411 382 278 1164 491 4545
Fragments arranged by size:
4545 1164 491 411 382 292 278 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2870 2903 2870
Size: 33 7603
BamHI G'GATC_C
Cuts at: 3408 4966 5333 3408
Size: 1558 367 5711
Fragments arranged by size:
5711 1558 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3557 3707 4886 4957 6412
Size: 325 217 627 1770 150 1179 71 1455
Cuts at: 6412 618
Size: 1842
Fragments arranged by size:
1842 1770 1455 1179 627 325 217 150
71
BanII G_rGCy'C
Cuts at: 1391 3035 3210 3386 3446 3924 4062 4752 4955
Size: 1644 175 176 60 478 138 690 203
Cuts at: 4955 1391
Size: 4072
Fragments arranged by size:
4072 1644 690 478 203 176 175 138
60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3972 4606 928
Size: 971 2073 634 3958
Fragments arranged by size:
3958 2073 971 634
BbvCI CC'TCA_GC
Cuts at: 2127 2127
Size: 7636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2192 2226 5827 5861 1903
Size: 34 255 34 3601 34 3678
Fragments arranged by size:
3678 3601 255 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2360 5528 6738 2360
Size: 3168 1210 3258
Fragments arranged by size:
3258 3168 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7636
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2844 6247 136
Size: 107 122 71 631 605 1172 3403 1525
Fragments arranged by size:
3403 1525 1172 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3669 7631 3669
Size: 3962 3674
BlpI GC'TnA_GC
Cuts at: 5053 5053
Size: 7636
Bme1580I G_kGCm'C
Cuts at: 1790 3035 3560 3783 4062 4826 5697 6853 1790
Size: 1245 525 223 279 764 871 1156 2573
Fragments arranged by size:
2573 1245 1156 871 764 525 279 223
BmgBI CAC'GTC
Cuts at: 1318 3535 3850 4620 1318
Size: 2217 315 770 4334
Fragments arranged by size:
4334 2217 770 315
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2399 2501 4938
Size: 416 53 83 399 24 982 102 2437
Cuts at: 4938 5367 6325 442
Size: 429 958 1753
Fragments arranged by size:
2437 1753 982 958 429 416 399 102
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7636
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3169 3169
Size: 7636
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 2926 4126 4467 4673 6296 1239
Size: 1091 596 1200 341 206 1623 2579
Fragments arranged by size:
2579 1623 1200 1091 596 341 206
Bpu10I CC'TnA_GC
Cuts at: 2127 2127
Size: 7636
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2087 3359 3788 3908 4127 5761 6626 6924 2087
Size: 1272 429 120 219 1634 865 298 2799
Fragments arranged by size:
2799 1634 1272 865 429 298 219 120
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3065 3140 6299 882
Size: 617 1566 75 3159 2219
Fragments arranged by size:
3159 2219 1566 617 75
BsaAI yAC'GTr
Cuts at: 493 1204 2638 5427 493
Size: 711 1434 2789 2702
Fragments arranged by size:
2789 2702 1434 711
BsaBI GATnn'nnATC
Cuts at: 7630 7630
Size: 7636
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2395 4632 5443 5825
Size: 53 83 186 936 862 2237 811 382
Cuts at: 5825 6460 275
Size: 635 1451
Fragments arranged by size:
2237 1451 936 862 811 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3378 6069 6467 6741 6888 7299 3378
Size: 2691 398 274 147 411 3715
Fragments arranged by size:
3715 2691 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 1178
Size: 30 165 30 828 30 6553
Fragments arranged by size:
6553 828 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3158 3539 3560 4364 4760 1186
Size: 1972 381 21 804 396 4062
Fragments arranged by size:
4062 1972 804 396 381 21
BseYI C'CCAG_C
Cuts at: 2329 2656 2744 2871 3589 4061 4641 6839 7335
Size: 327 88 127 718 472 580 2198 496
Cuts at: 7335 2329
Size: 2630
Fragments arranged by size:
2630 2198 718 580 496 472 327 127
88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2132 2195 2437 3487 1163
Size: 573 396 63 242 1050 5312
Fragments arranged by size:
5312 1050 573 396 242 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4303 5086 5847 6875 1540
Size: 275 186 1372 930 783 761 1028 2301
Fragments arranged by size:
2301 1372 1028 930 783 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3184 3345 3386 3642 3783 3837 3924 4045 4752
Size: 161 41 256 141 54 87 121 707
Cuts at: 4752 5697 5782 6853 3184
Size: 945 85 1071 3967
Fragments arranged by size:
3967 1071 945 707 256 161 141 121
87 85 54 41
BsmI GAATG_Cn'
Cuts at: 5147 5240 5147
Size: 93 7543
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3035 3184 3210 3345 3386 3446 3560
Size: 399 1245 149 26 135 41 60 114
Cuts at: 3560 3642 3783 3837 3924 4045 4062 4752 4826
Size: 82 141 54 87 121 17 690 74
Cuts at: 4826 4955 5697 5782 6853 1391
Size: 129 742 85 1071 2174
Fragments arranged by size:
2174 1245 1071 742 690 399 149 141
135 129 121 114 87 85 82 74
60 54 41 26 17
BspEI T'CCGG_A
Cuts at: 3378 7299 3378
Size: 3921 3715
BspHI T'CATG_A
Cuts at: 4371 4518 5525 7255 4371
Size: 147 1007 1730 4752
Fragments arranged by size:
4752 1730 1007 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2718 3063 3582 4083 4108 4991 844
Size: 1874 345 519 501 25 883 3489
Fragments arranged by size:
3489 1874 883 519 501 345 25
BsrBI CCG'CTC
Cuts at: 1394 1823 3550 3792 4186 5523 1394
Size: 429 1727 242 394 1337 3507
Fragments arranged by size:
3507 1727 1337 429 394 242
BsrDI GCAATG_nn'
Cuts at: 65 1248 2140 6131 6305 65
Size: 1183 892 3991 174 1396
Fragments arranged by size:
3991 1396 1183 892 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2424 3372 4767 4883 6280 6467 1700
Size: 110 614 948 1395 116 1397 187 2869
Fragments arranged by size:
2869 1397 1395 948 614 187 116 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7636
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7636
BssSI C'ACGA_G
Cuts at: 3108 3260 5696 6708 3108
Size: 152 2436 1012 4036
Fragments arranged by size:
4036 2436 1012 152
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4704 794
Size: 3910 3726
BstBI TT'CG_AA
Cuts at: 6440 6440
Size: 7636
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7636
BstXI CCAn_nnnn'nTGG
Cuts at: 2995 3439 2995
Size: 444 7192
BstYI r'GATC_y
Cuts at: 1972 3408 3669 3816 4386 4488 4966 5333 5718
Size: 1436 261 147 570 102 478 367 385
Cuts at: 5718 5735 7187 7273 7285 7631 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1436 570 478 385 367 346
261 147 102 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2286 4500 4659 4735 4826 513
Size: 552 939 282 2214 159 76 91 3323
Fragments arranged by size:
3323 2214 939 552 282 159 91 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3604 487
Size: 683 56 2378 4519
Fragments arranged by size:
4519 2378 683 56
BtsI GCAGTG_nn'
Cuts at: 2562 2903 2948 3222 4168 4241 4531 4577 5152
Size: 341 45 274 946 73 290 46 575
Cuts at: 5152 5946 5966 2562
Size: 794 20 4232
Fragments arranged by size:
4232 946 794 575 341 290 274 73
46 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7636
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7601
DraI TTT'AAA
Cuts at: 4940 4940
Size: 7636
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2780 6643 809
Size: 507 1464 3863 1802
Fragments arranged by size:
3863 1802 1464 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2997 3370
Size: 54 1425 50 275 18 369 798 373
Cuts at: 3370 4096 4300 4794 5083 5972 7601 8
Size: 726 204 494 289 889 1629 43
Fragments arranged by size:
1629 1425 889 798 726 494 373 369
289 275 204 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 4300 5083 1537
Size: 275 1558 930 783 4090
Fragments arranged by size:
4090 1558 930 783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3584 5566 1353
Size: 2231 1982 3423
Fragments arranged by size:
3423 2231 1982
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 3809 6223 6597 6743 387
Size: 1064 138 453 1767 2414 374 146 1280
Fragments arranged by size:
2414 1767 1280 1064 453 374 146 138
EcoICRI GAG'CTC
Cuts at: 3384 3922 4750 3384
Size: 538 828 6270
Fragments arranged by size:
6270 828 538
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2926 3653 4126 4467 4673 4802
Size: 393 698 596 727 473 341 206 129
Cuts at: 4802 5699 6296 7083 7351 1239
Size: 897 597 787 268 1524
Fragments arranged by size:
1524 897 787 727 698 597 596 473
393 341 268 206 129
EcoNI CCTnn'n_nnAGG
Cuts at: 3884 4449 3884
Size: 565 7071
EcoO109I rG'GnC_Cy
Cuts at: 2523 2736 2898 3032 3302 3334 4138 4192 4455
Size: 213 162 134 270 32 804 54 263
Cuts at: 4455 4876 2523
Size: 421 5283
Fragments arranged by size:
5283 804 421 270 263 213 162 134
54 32
EcoRI G'AATT_C
Cuts at: 4945 5323 4945
Size: 378 7258
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3262 3451 3772 3262
Size: 189 321 7126
Fragments arranged by size:
7126 321 189
FspI TGC'GCA
Cuts at: 6142 6142
Size: 7636
HaeII r_GCGC'y
Cuts at: 1678 2309 2330 6783 1678
Size: 631 21 4453 2531
Fragments arranged by size:
4453 2531 631 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2753 2785 2804 2836 3707
Size: 32 135 32 1181 32 19 32 871
Cuts at: 3707 3739 6277 6309 6351 6383 1373
Size: 32 2538 32 42 32 2626
Fragments arranged by size:
2626 2538 1181 871 135 42 32 32
32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 2061 3105 4980 5226 669
Size: 988 281 123 1044 1875 246 3079
Fragments arranged by size:
3079 1875 1044 988 281 246 123
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7636
HpaI GTT'AAC
Cuts at: 5226 5226
Size: 7636
KpnI G_GTAC'C
Cuts at: 4961 4961
Size: 7636
MfeI C'AATT_G
Cuts at: 5235 5235
Size: 7636
MluI A'CGCG_T
Cuts at: 7512 7512
Size: 7636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3759 4647 6750 6934 1305
Size: 2454 888 2103 184 2007
Fragments arranged by size:
2454 2103 2007 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4098 4796 10
Size: 54 1425 343 2266 698 2850
Fragments arranged by size:
2850 2266 1425 698 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4520 5594 5953 6112 518
Size: 648 3354 1074 359 159 2042
Fragments arranged by size:
3354 2042 1074 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2850 2875 3589 3894 4638 4728 5052 5729
Size: 1244 25 714 305 744 90 324 677
Cuts at: 5729 6877 7122 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1244 1148 744 714 677 324 305
245 90 25
NaeI GCC'GGC
Cuts at: 3374 3374
Size: 7636
NcoI C'CATG_G
Cuts at: 513 1065 2004 2286 4659 4735 513
Size: 552 939 282 2373 76 3414
Fragments arranged by size:
3414 2373 939 552 282 76
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7636
NgoMIV G'CCGG_C
Cuts at: 3372 3372
Size: 7636
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7636
NotI GC'GGCC_GC
Cuts at: 5083 5083
Size: 7636
NspI r_CATG'y
Cuts at: 1132 1229 2261 3217 4016 4363 4399 4417 4816
Size: 97 1032 956 799 347 36 18 399
Cuts at: 4816 4994 6539 1132
Size: 178 1545 2229
Fragments arranged by size:
2229 1545 1032 956 799 399 347 178
97 36 18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7636
PciI A'CATG_T
Cuts at: 4359 4413 6535 4359
Size: 54 2122 5460
Fragments arranged by size:
5460 2122 54
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2491 2533 4318 4641 1108
Size: 1383 42 1785 323 4103
Fragments arranged by size:
4103 1785 1383 323 42
PfoI T'CCnGG_A
Cuts at: 2145 2808 3828 4389 2145
Size: 663 1020 561 5392
Fragments arranged by size:
5392 1020 663 561
PmeI GTTT'AAAC
Cuts at: 4940 4940
Size: 7636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5705 5737 7218 7250 5705
Size: 32 1481 32 6091
Fragments arranged by size:
6091 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2736 2898 4138 4192 4455 4876 2736
Size: 162 1240 54 263 421 5496
Fragments arranged by size:
5496 1240 421 263 162 54
PshAI GACnn'nnGTC
Cuts at: 1613 3165 1613
Size: 1552 6084
PsiI TTA'TAA
Cuts at: 5206 5206
Size: 7636
PspOMI G'GGCC_C
Cuts at: 3031 4058 3031
Size: 1027 6609
PspXI vC'TCGA_Gb
Cuts at: 2646 2646
Size: 7636
PstI C_TGCA'G
Cuts at: 830 2049 3942 4080 4988 830
Size: 1219 1893 138 908 3478
Fragments arranged by size:
3478 1893 1219 908 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7636
PvuII CAG'CTG
Cuts at: 2875 3894 2875
Size: 1019 6617
SacI G_AGCT'C
Cuts at: 3386 3924 4752 3386
Size: 538 828 6270
Fragments arranged by size:
6270 828 538
SalI G'TCGA_C
Cuts at: 1936 2059 3103 4978 1936
Size: 123 1044 1875 4594
Fragments arranged by size:
4594 1875 1044 123
SanDI GG'GwC_CC
Cuts at: 2898 4455 2898
Size: 1557 6079
SapI GCTCTTCn'nnn_
Cuts at: 3584 3584
Size: 7636
SbfI CC_TGCA'GG
Cuts at: 4988 4988
Size: 7636
ScaI AGT'ACT
Cuts at: 3809 3947 3992 4148 5884 7596 3809
Size: 138 45 156 1736 1712 3849
Fragments arranged by size:
3849 1736 1712 156 138 45
SexAI A'CCwGG_T
Cuts at: 2220 2220
Size: 7636
SfcI C'TryA_G
Cuts at: 826 1045 2045 3938 4076 4150 4984 6119 6800
Size: 219 1000 1893 138 74 834 1135 681
Cuts at: 6800 6991 826
Size: 191 1471
Fragments arranged by size:
1893 1471 1135 1000 834 681 219 191
138 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7636
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7636
SmaI CCC'GGG
Cuts at: 1872 2237 3030 3308 4963 1872
Size: 365 793 278 1655 4545
Fragments arranged by size:
4545 1655 793 365 278
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2102 2646 3338 3803 3923 4142
Size: 197 926 159 544 692 465 120 219
Cuts at: 4142 5740 6641 6903 820
Size: 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 692 544 465 262
219 197 159 120
SnaBI TAC'GTA
Cuts at: 493 5427 493
Size: 4934 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7636
SphI G_CATG'C
Cuts at: 1132 3217 4016 4994 1132
Size: 2085 799 978 3774
Fragments arranged by size:
3774 2085 978 799
SspI AAT'ATT
Cuts at: 5 52 5560 5
Size: 47 5508 2081
Fragments arranged by size:
5508 2081 47
StuI AGG'CCT
Cuts at: 1517 2093 2408 3275 1517
Size: 576 315 867 5878
Fragments arranged by size:
5878 867 576 315
StyI C'CwwG_G
Cuts at: 513 1065 2004 2286 3246 3711 4158 4179 4209
Size: 552 939 282 960 465 447 21 30
Cuts at: 4209 4659 4735 4797 5075 513
Size: 450 76 62 278 3074
Fragments arranged by size:
3074 960 939 552 465 450 447 282
278 76 62 30 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2912 3272 3557 4028 4216 5663 5680
Size: 1255 1332 360 285 471 188 1447 17
Cuts at: 5680 5833 6018 6463 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1447 1332 1255 471 445 360 285
188 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 3807 3945
Size: 274 80 33 51 1443 201 1629 138
Cuts at: 3945 3990 4146 5882 7594 96
Size: 45 156 1736 1712 138
Fragments arranged by size:
1736 1712 1629 1443 274 201 156 138
138 80 51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2231 2263 4574 4606 7596 7628 2231
Size: 32 2311 32 2990 32 2239
Fragments arranged by size:
2990 2311 2239 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7636
XbaI T'CTAG_A
Cuts at: 4972 4972
Size: 7636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4033 4033
Size: 7636
XhoI C'TCGA_G
Cuts at: 1943 2646 1943
Size: 703 6933
XmaI C'CCGG_G
Cuts at: 1870 2235 3028 3306 4961 1870
Size: 365 793 278 1655 4545
Fragments arranged by size:
4545 1655 793 365 278
XmnI GAAnn'nnTTC
Cuts at: 4613 5765 4613
Size: 1152 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5444 276
Size: 53 83 186 936 3910 2468
Fragments arranged by size:
3910 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PsrI RsrII SacII SfiI
SfoI SrfI SwaI