Restriction map for
pFN21AA1017
(Circular) (Six-base) MAPSORT of: pFN21AA1017.seq Check: 1161 from: 1 to: 7750
pFN21AA1017 7750 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5560 278
Size: 53 83 186 936 4024 2468
Fragments arranged by size:
4024 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5093 1937
Size: 3156 4594
Acc65I G'GTAC_C
Cuts at: 5071 5071
Size: 7750
AclI AA'CG_TT
Cuts at: 1563 5877 6250 1563
Size: 4314 373 3063
Fragments arranged by size:
4314 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2001 3263 4016 4389 5813 7197 7465 1632
Size: 369 1262 753 373 1424 1384 268 1917
Fragments arranged by size:
1917 1424 1384 1262 753 373 369 268
AfeI AGC'GCT
Cuts at: 2738 2738
Size: 7750
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7553
AflIII A'CryG_T
Cuts at: 1203 3940 6649 7626 1203
Size: 2737 2709 977 1327
Fragments arranged by size:
2737 2709 1327 977
AgeI A'CCGG_T
Cuts at: 6581 6581
Size: 7750
AhdI GACnn_n'nnGTC
Cuts at: 6479 6479
Size: 7750
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7750
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7750
AlwNI CAG_nnn'CTG
Cuts at: 2147 3647 3994 4956 5493 7065 2147
Size: 1500 347 962 537 1572 2832
Fragments arranged by size:
2832 1572 1500 962 537 347
ApaI G_GGCC'C
Cuts at: 4886 4886
Size: 7750
ApaLI G'TGCA_C
Cuts at: 5807 6963 5807
Size: 1156 6594
ApoI r'AATT_y
Cuts at: 1493 2592 2806 2930 3110 3913 3975 5059 5285
Size: 1099 214 124 180 803 62 1084 226
Cuts at: 5285 5437 7546 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1099 1084 803 226 214 180
152 124 62
AseI AT'TA_AT
Cuts at: 160 1032 6304 160
Size: 872 5272 1606
Fragments arranged by size:
5272 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7750
AvaI C'yCGr_G
Cuts at: 1870 1943 5075 1870
Size: 73 3132 4545
Fragments arranged by size:
4545 3132 73
BamHI G'GATC_C
Cuts at: 2516 4725 5080 5447 2516
Size: 2209 355 367 4819
Fragments arranged by size:
4819 2209 367 355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2885 3335 5071 6526 618
Size: 325 217 627 1098 450 1736 1455 1842
Fragments arranged by size:
1842 1736 1455 1098 627 450 325 217
BanII G_rGCy'C
Cuts at: 1391 3353 3378 4886 5069 1391
Size: 1962 25 1508 183 4072
Fragments arranged by size:
4072 1962 1508 183 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2007 3063 3177 3444 3489 3851 928
Size: 971 108 1056 114 267 45 362 4827
Fragments arranged by size:
4827 1056 971 362 267 114 108 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5941 5975 1903
Size: 34 4004 34 3678
Fragments arranged by size:
4004 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2050 4072 5642 6852 2050
Size: 2022 1570 1210 2948
Fragments arranged by size:
2948 2022 1570 1210
BclI T'GATC_A
Cuts at: 1548 2086 2503 1548
Size: 538 417 6795
Fragments arranged by size:
6795 538 417
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6361 136
Size: 107 122 71 631 605 4689 1525
Fragments arranged by size:
4689 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4147 7745 4147
Size: 3598 4152
BlpI GC'TnA_GC
Cuts at: 5167 5167
Size: 7750
Bme1580I G_kGCm'C
Cuts at: 1790 4886 5811 6967 1790
Size: 3096 925 1156 2573
Fragments arranged by size:
3096 2573 1156 925
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7750
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2518 5481 6439
Size: 416 53 83 399 24 1101 2963 958
Cuts at: 6439 442
Size: 1753
Fragments arranged by size:
2963 1753 1101 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3174 3459 4670 6410 1239
Size: 1935 285 1211 1740 2579
Fragments arranged by size:
2579 1935 1740 1211 285
Bpu10I CC'TnA_GC
Cuts at: 3377 4872 3377
Size: 1495 6255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4412 4949 5875 6740 7038 4412
Size: 537 926 865 298 5124
Fragments arranged by size:
5124 926 865 537 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3361 4727 6413 882
Size: 617 1862 1366 1686 2219
Fragments arranged by size:
2219 1862 1686 1366 617
BsaAI yAC'GTr
Cuts at: 493 1204 5541 493
Size: 711 4337 2702
Fragments arranged by size:
4337 2702 711
BsaBI GATnn'nnATC
Cuts at: 7744 7744
Size: 7750
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5557 5939 6574 275
Size: 53 83 186 936 4024 382 635 1451
Fragments arranged by size:
4024 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6183 6581 6855 7002 7413 6183
Size: 398 274 147 411 6520
Fragments arranged by size:
6520 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3050 3080 4807 4837 1178
Size: 30 165 30 1647 30 1727 30 4091
Fragments arranged by size:
4091 1727 1647 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 3255 3461 1186
Size: 1255 814 206 5475
Fragments arranged by size:
5475 1255 814 206
BseYI C'CCAG_C
Cuts at: 2052 2093 5040 6953 7449 2052
Size: 41 2947 1913 496 2353
Fragments arranged by size:
2947 2353 1913 496 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5026 5200 5961 6989 1540
Size: 275 186 3025 174 761 1028 2301
Fragments arranged by size:
3025 2301 1028 761 275 186 174
BsiHKAI G_wGCw'C
Cuts at: 2380 3378 4784 5811 5896 6967 2380
Size: 998 1406 1027 85 1071 3163
Fragments arranged by size:
3163 1406 1071 1027 998 85
BsmI GAATG_Cn'
Cuts at: 3237 5261 5354 3237
Size: 2024 93 5633
Fragments arranged by size:
5633 2024 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2380 3353 3378 4784 4886 5069 5811
Size: 399 590 973 25 1406 102 183 742
Cuts at: 5811 5896 6967 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1406 1071 973 742 590 399 183
102 85 25
BspEI T'CCGG_A
Cuts at: 7413 7413
Size: 7750
BspHI T'CATG_A
Cuts at: 5639 7369 5639
Size: 1730 6020
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3144 3431 4593 4964 5105 844
Size: 2300 287 1162 371 141 3489
Fragments arranged by size:
3489 2300 1162 371 287 141
BsrBI CCG'CTC
Cuts at: 1394 1823 5637 1394
Size: 429 3814 3507
Fragments arranged by size:
3814 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2814 3528 3760 3846 6245 6419 65
Size: 1183 1566 714 232 86 2399 174 1396
Fragments arranged by size:
2399 1566 1396 1183 714 232 174 86
BsrFI r'CCGG_y
Cuts at: 1700 1810 6394 6581 1700
Size: 110 4584 187 2869
Fragments arranged by size:
4584 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2200 96
Size: 2104 5646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7750
BssSI C'ACGA_G
Cuts at: 3346 4783 5810 6822 3346
Size: 1437 1027 1012 4274
Fragments arranged by size:
4274 1437 1027 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2147 2569 794
Size: 1353 422 5975
Fragments arranged by size:
5975 1353 422
BstBI TT'CG_AA
Cuts at: 6554 6554
Size: 7750
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7750
BstXI CCAn_nnnn'nTGG
Cuts at: 4477 4846 4477
Size: 369 7381
BstYI r'GATC_y
Cuts at: 1972 2516 4147 4725 5080 5447 5832 5849 7301
Size: 544 1631 578 355 367 385 17 1452
Cuts at: 7301 7387 7399 7745 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1631 1452 578 544 385 367 355
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4967 4967
Size: 7750
BtgI C'CryG_G
Cuts at: 513 1065 2881 4737 4878 513
Size: 552 1816 1856 141 3385
Fragments arranged by size:
3385 1856 1816 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7011
Fragments arranged by size:
7011 683 56
BtsI GCAGTG_nn'
Cuts at: 4449 4901 5266 6060 6080 4449
Size: 452 365 794 20 6119
Fragments arranged by size:
6119 794 452 365 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7750
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7715
DraI TTT'AAA
Cuts at: 3106 5054 3106
Size: 1948 5802
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6757 809
Size: 507 5441 1802
Fragments arranged by size:
5441 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5197 6086 7715
Size: 54 1425 50 275 18 3367 889 1629
Cuts at: 7715 8
Size: 43
Fragments arranged by size:
3367 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5197 1537
Size: 275 3385 4090
Fragments arranged by size:
4090 3385 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2029 2755 3031 3123 3282 3396 4257 4654
Size: 676 726 276 92 159 114 861 397
Cuts at: 4654 5680 1353
Size: 1026 3423
Fragments arranged by size:
3423 1026 861 726 676 397 276 159
114 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6337 6711 6857 387
Size: 1064 138 4748 374 146 1280
Fragments arranged by size:
4748 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3376 3376
Size: 7750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2001 3174 3263 3459 4016 4389 4670
Size: 393 369 1173 89 196 557 373 281
Cuts at: 4670 5813 6410 7197 7465 1239
Size: 1143 597 787 268 1524
Fragments arranged by size:
1524 1173 1143 787 597 557 393 373
369 281 268 196 89
EcoO109I rG'GnC_Cy
Cuts at: 3133 3538 4992 3133
Size: 405 1454 5891
Fragments arranged by size:
5891 1454 405
EcoRI G'AATT_C
Cuts at: 3110 3913 5059 5437 3110
Size: 803 1146 378 5423
Fragments arranged by size:
5423 1146 803 378
EcoRV GAT'ATC
Cuts at: 2727 4297 4628 4963 2727
Size: 1570 331 335 5514
Fragments arranged by size:
5514 1570 335 331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2645 2853 3762 3825 4021 2645
Size: 208 909 63 196 6374
Fragments arranged by size:
6374 909 208 196 63
FspI TGC'GCA
Cuts at: 6256 6256
Size: 7750
HaeII r_GCGC'y
Cuts at: 1678 2740 6897 1678
Size: 1062 4157 2531
Fragments arranged by size:
4157 2531 1062
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3044 3050 3076 3082 3340
Size: 32 135 32 1472 6 26 6 258
Cuts at: 3340 3360 3372 3377 3392 3409 3641 3673 4248
Size: 20 12 5 15 17 232 32 575
Cuts at: 4248 4280 4364 4396 4531 4563 6391 6423 6465
Size: 32 84 32 135 32 1828 32 42
Cuts at: 6465 6497 1373
Size: 32 2626
Fragments arranged by size:
2626 1828 1472 575 258 232 135 135
84 42 32 32 32 32 32 32
32 32 26 20 17 15 12 6
6 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 5094 5340 669
Size: 988 281 250 2906 246 3079
Fragments arranged by size:
3079 2906 988 281 250 246
HindIII A'AGCT_T
Cuts at: 748 4046 4751 748
Size: 3298 705 3747
Fragments arranged by size:
3747 3298 705
HpaI GTT'AAC
Cuts at: 5340 5340
Size: 7750
KpnI G_GTAC'C
Cuts at: 5075 5075
Size: 7750
MfeI C'AATT_G
Cuts at: 5349 5349
Size: 7750
MluI A'CGCG_T
Cuts at: 7626 7626
Size: 7750
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4404 4703 6864 7048 1305
Size: 3099 299 2161 184 2007
Fragments arranged by size:
3099 2161 2007 299 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5928
Fragments arranged by size:
5928 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2556 2697 3165 3784 4844 5708
Size: 648 843 547 141 468 619 1060 864
Cuts at: 5708 6067 6226 518
Size: 359 159 2042
Fragments arranged by size:
2042 1060 864 843 648 619 547 468
359 159 141
MspA1I CmG'CkG
Cuts at: 1606 2144 2513 2875 4378 4537 5166 5843 6991
Size: 538 369 362 1503 159 629 677 1148
Cuts at: 6991 7236 1606
Size: 245 2120
Fragments arranged by size:
2120 1503 1148 677 629 538 369 362
245 159
NcoI C'CATG_G
Cuts at: 513 1065 2881 4878 513
Size: 552 1816 1997 3385
Fragments arranged by size:
3385 1997 1816 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7750
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7750
NotI GC'GGCC_GC
Cuts at: 5197 5197
Size: 7750
NspI r_CATG'y
Cuts at: 1132 1229 2793 3783 3944 5011 5108 6653 1132
Size: 97 1564 990 161 1067 97 1545 2229
Fragments arranged by size:
2229 1564 1545 1067 990 161 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7750
PciI A'CATG_T
Cuts at: 3940 6649 3940
Size: 2709 5041
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7750
PfoI T'CCnGG_A
Cuts at: 2335 3492 2335
Size: 1157 6593
PmeI GTTT'AAAC
Cuts at: 5054 5054
Size: 7750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5819 5851 7332 7364 5819
Size: 32 1481 32 6205
Fragments arranged by size:
6205 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3133 3538 3133
Size: 405 7345
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7750
PsiI TTA'TAA
Cuts at: 2426 4574 5320 2426
Size: 2148 746 4856
Fragments arranged by size:
4856 2148 746
PspOMI G'GGCC_C
Cuts at: 4882 4882
Size: 7750
PstI C_TGCA'G
Cuts at: 830 2848 4567 4604 5102 830
Size: 2018 1719 37 498 3478
Fragments arranged by size:
3478 2018 1719 498 37
PvuI CG_AT'CG
Cuts at: 2001 5026 2001
Size: 3025 4725
PvuII CAG'CTG
Cuts at: 2144 2513 2875 4537 2144
Size: 369 362 1662 5357
Fragments arranged by size:
5357 1662 369 362
SacI G_AGCT'C
Cuts at: 3378 3378
Size: 7750
SalI G'TCGA_C
Cuts at: 1936 5092 1936
Size: 3156 4594
SapI GCTCTTCn'nnn_
Cuts at: 4257 4257
Size: 7750
SbfI CC_TGCA'GG
Cuts at: 5102 5102
Size: 7750
ScaI AGT'ACT
Cuts at: 3965 4004 5998 7710 3965
Size: 39 1994 1712 4005
Fragments arranged by size:
4005 1994 1712 39
SfcI C'TryA_G
Cuts at: 826 1045 2668 2844 3533 4563 4600 4907 5098
Size: 219 1623 176 689 1030 37 307 191
Cuts at: 5098 6233 6914 7105 826
Size: 1135 681 191 1471
Fragments arranged by size:
1623 1471 1135 1030 689 681 307 219
191 191 176 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7750
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7750
SmaI CCC'GGG
Cuts at: 1872 5077 1872
Size: 3205 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4391 4928 5854 6755 7017 820
Size: 197 926 2448 537 926 901 262 1553
Fragments arranged by size:
2448 1553 926 926 901 537 262 197
SnaBI TAC'GTA
Cuts at: 493 5541 493
Size: 5048 2702
SpeI A'CTAG_T
Cuts at: 152 3464 152
Size: 3312 4438
SphI G_CATG'C
Cuts at: 1132 5011 5108 1132
Size: 3879 97 3774
Fragments arranged by size:
3879 3774 97
SspI AAT'ATT
Cuts at: 5 52 3711 4678 5674 5
Size: 47 3659 967 996 2081
Fragments arranged by size:
3659 2081 996 967 47
StuI AGG'CCT
Cuts at: 1517 2766 4366 4501 4667 1517
Size: 1249 1600 135 166 4600
Fragments arranged by size:
4600 1600 1249 166 135
StyI C'CwwG_G
Cuts at: 513 1065 2881 3800 4878 5189 513
Size: 552 1816 919 1078 311 3074
Fragments arranged by size:
3074 1816 1078 919 552 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5777 5794 5947 6132 6577 325
Size: 1255 4197 17 153 185 445 1498
Fragments arranged by size:
4197 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2200 2429 3963
Size: 274 80 33 51 1443 223 229 1534
Cuts at: 3963 4002 5996 7708 96
Size: 39 1994 1712 138
Fragments arranged by size:
1994 1712 1534 1443 274 229 223 138
80 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4502 4534 4807 4839 7710 7742 4502
Size: 32 273 32 2871 32 4510
Fragments arranged by size:
4510 2871 273 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7750
XbaI T'CTAG_A
Cuts at: 5086 5086
Size: 7750
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3940 4893 3940
Size: 953 6797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7750
XmaI C'CCGG_G
Cuts at: 1870 5075 1870
Size: 3205 4545
XmnI GAAnn'nnTTC
Cuts at: 2344 3222 5879 2344
Size: 878 2657 4215
Fragments arranged by size:
4215 2657 878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5558 276
Size: 53 83 186 936 4024 2468
Fragments arranged by size:
4024 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI