Restriction map for
pFN21AA1003
(Circular) (Six-base) MAPSORT of: pFN21AA1003.seq Check: 9847 from: 1 to: 7444
pFN21AA1003 7444 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4075 4075
Size: 7444
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5254 278
Size: 53 83 186 936 3718 2468
Fragments arranged by size:
3718 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4787 1937
Size: 2850 4594
Acc65I G'GTAC_C
Cuts at: 4765 4765
Size: 7444
AclI AA'CG_TT
Cuts at: 1563 3413 5571 5944 1563
Size: 1850 2158 373 3063
Fragments arranged by size:
3063 2158 1850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4598 5507 6891 7159 1632
Size: 2966 909 1384 268 1917
Fragments arranged by size:
2966 1917 1384 909 268
AfeI AGC'GCT
Cuts at: 3320 3764 3320
Size: 444 7000
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7247
AflIII A'CryG_T
Cuts at: 1203 3669 4134 4335 6343 7320 1203
Size: 2466 465 201 2008 977 1327
Fragments arranged by size:
2466 2008 1327 977 465 201
AgeI A'CCGG_T
Cuts at: 6275 6275
Size: 7444
AhdI GACnn_n'nnGTC
Cuts at: 6173 6173
Size: 7444
AleI CACnn'nnGTG
Cuts at: 1166 3404 1166
Size: 2238 5206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3743 4481 1184
Size: 2559 738 4147
Fragments arranged by size:
4147 2559 738
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 7412
AlwNI CAG_nnn'CTG
Cuts at: 2290 2491 2587 3123 4687 5187 6759 2290
Size: 201 96 536 1564 500 1572 2975
Fragments arranged by size:
2975 1572 1564 536 500 201 96
ApaI G_GGCC'C
Cuts at: 4527 4694 4527
Size: 167 7277
ApaLI G'TGCA_C
Cuts at: 3859 5501 6657 3859
Size: 1642 1156 4646
Fragments arranged by size:
4646 1642 1156
ApoI r'AATT_y
Cuts at: 1493 4753 4979 5131 7240 1493
Size: 3260 226 152 2109 1697
Fragments arranged by size:
3260 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3957 5998 160
Size: 872 2925 2041 1606
Fragments arranged by size:
2925 2041 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
AvaI C'yCGr_G
Cuts at: 1870 1943 2427 2453 2784 2866 3737 3821 4520
Size: 73 484 26 331 82 871 84 699
Cuts at: 4520 4652 4769 1870
Size: 132 117 4545
Fragments arranged by size:
4545 871 699 484 331 132 117 84
82 73 26
BamHI G'GATC_C
Cuts at: 4774 5141 4774
Size: 367 7077
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3253 3503 4765 6220 618
Size: 325 217 627 1466 250 1262 1455 1842
Fragments arranged by size:
1842 1466 1455 1262 627 325 250 217
BanII G_rGCy'C
Cuts at: 1391 2312 2745 2837 3831 4116 4518 4527 4694
Size: 921 433 92 994 285 402 9 167
Cuts at: 4694 4763 1391
Size: 69 4072
Fragments arranged by size:
4072 994 921 433 402 285 167 92
69 9
BbeI G_GCGC'C
Cuts at: 3507 3507
Size: 7444
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3627 928
Size: 971 1728 4745
Fragments arranged by size:
4745 1728 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3188 3222 4369 4403 5635 5669 1903
Size: 34 1251 34 1147 34 1232 34 3678
Fragments arranged by size:
3678 1251 1232 1147 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3406 3586 5336 6546 3406
Size: 180 1750 1210 4304
Fragments arranged by size:
4304 1750 1210 180
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7444
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2439 2486 2768
Size: 107 122 71 631 605 767 47 282
Cuts at: 2768 3909 4427 4522 6055 136
Size: 1141 518 95 1533 1525
Fragments arranged by size:
1533 1525 1141 767 631 605 518 282
122 107 95 71 47
BglII A'GATC_T
Cuts at: 7439 7439
Size: 7444
BlpI GC'TnA_GC
Cuts at: 4861 4861
Size: 7444
Bme1580I G_kGCm'C
Cuts at: 1790 3863 3930 4527 4694 5505 6661 1790
Size: 2073 67 597 167 811 1156 2573
Fragments arranged by size:
2573 2073 1156 811 597 167 67
BmgBI CAC'GTC
Cuts at: 1318 3808 1318
Size: 2490 4954
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5175 6133 442
Size: 416 53 83 399 24 3758 958 1753
Fragments arranged by size:
3758 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 2300 3100 6104 1239
Size: 760 301 800 3004 2579
Fragments arranged by size:
3004 2579 800 760 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 3254 5569 6434 6732 2729
Size: 525 2315 865 298 3441
Fragments arranged by size:
3441 2315 865 525 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2596 4607 6107 882
Size: 617 1097 2011 1500 2219
Fragments arranged by size:
2219 2011 1500 1097 617
BsaAI yAC'GTr
Cuts at: 493 1204 2626 3265 4036 4135 4336 5235 493
Size: 711 1422 639 771 99 201 899 2702
Fragments arranged by size:
2702 1422 899 771 711 639 201 99
BsaBI GATnn'nnATC
Cuts at: 4391 4661 7438 4391
Size: 270 2777 4397
Fragments arranged by size:
4397 2777 270
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3131 3504 4446 5251
Size: 53 83 186 936 1598 373 942 805
Cuts at: 5251 5633 6268 275
Size: 382 635 1451
Fragments arranged by size:
1598 1451 942 936 805 635 382 373
186 83 53
BsaWI w'CCGG_w
Cuts at: 2111 2829 5877 6275 6549 6696 7107 2111
Size: 718 3048 398 274 147 411 2448
Fragments arranged by size:
3048 2448 718 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 4277 4307 4325
Size: 30 165 30 621 30 2223 30 18
Cuts at: 4325 4355 1178
Size: 30 4267
Fragments arranged by size:
4267 2223 621 165 30 30 30 30
30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2302 2753 3157 3878 4076 4448 4625 1186
Size: 1116 451 404 721 198 372 177 4005
Fragments arranged by size:
4005 1116 721 451 404 372 198 177
BseYI C'CCAG_C
Cuts at: 2305 2688 3093 3587 4115 4687 6647 7143 2305
Size: 383 405 494 528 572 1960 496 2606
Fragments arranged by size:
2606 1960 572 528 496 494 405 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2717 3392 4037 4688 1163
Size: 573 981 675 645 651 3919
Fragments arranged by size:
3919 981 675 651 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4894 5655 6683 1540
Size: 275 186 2893 761 1028 2301
Fragments arranged by size:
2893 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2745 3270 3863 4290 4343 5505 5590 6661 2745
Size: 525 593 427 53 1162 85 1071 3528
Fragments arranged by size:
3528 1162 1071 593 525 427 85 53
BsiWI C'GTAC_G
Cuts at: 3261 3261
Size: 7444
BsmI GAATG_Cn'
Cuts at: 3750 4955 5048 3750
Size: 1205 93 6146
Fragments arranged by size:
6146 1205 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2921 3814 4001 4724 2921
Size: 893 187 723 5641
Fragments arranged by size:
5641 893 723 187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2312 2745 2837 3270 3831 3863 3930
Size: 399 522 433 92 433 561 32 67
Cuts at: 3930 4116 4290 4343 4518 4527 4694 4763 5505
Size: 186 174 53 175 9 167 69 742
Cuts at: 5505 5590 6661 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 561 522 433 433 399
186 175 174 167 92 85 69 67
53 32 9
BspEI T'CCGG_A
Cuts at: 7107 7107
Size: 7444
BspHI T'CATG_A
Cuts at: 5333 7063 5333
Size: 1730 5714
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2128 3843 4075 4710 4799 844
Size: 1284 1715 232 635 89 3489
Fragments arranged by size:
3489 1715 1284 635 232 89
BsrBI CCG'CTC
Cuts at: 1394 1823 2980 3685 5331 1394
Size: 429 1157 705 1646 3507
Fragments arranged by size:
3507 1646 1157 705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5939 6113 65
Size: 1183 4691 174 1396
Fragments arranged by size:
4691 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2752 3490 6088 6275 1700
Size: 110 942 738 2598 187 2869
Fragments arranged by size:
2869 2598 942 738 187 110
BsrGI T'GTAC_A
Cuts at: 96 3672 4293 96
Size: 3576 621 3247
Fragments arranged by size:
3576 3247 621
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7444
BssSI C'ACGA_G
Cuts at: 2735 3197 3231 3368 3773 4004 5504 6516 2735
Size: 462 34 137 405 231 1500 1012 3663
Fragments arranged by size:
3663 1500 1012 462 405 231 137 34
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7444
BstBI TT'CG_AA
Cuts at: 6248 6248
Size: 7444
BstEII G'GTnAC_C
Cuts at: 1184 2053 2107 2825 1184
Size: 869 54 718 5803
Fragments arranged by size:
5803 869 718 54
BstXI CCAn_nnnn'nTGG
Cuts at: 2124 3406 2124
Size: 1282 6162
BstYI r'GATC_y
Cuts at: 1972 3741 4774 5141 5526 5543 6995 7081 7093
Size: 1769 1033 367 385 17 1452 86 12
Cuts at: 7093 7439 1972
Size: 346 1977
Fragments arranged by size:
1977 1769 1452 1033 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2763 3063 3084 3107 3907 4213
Size: 552 939 759 300 21 23 800 306
Cuts at: 4213 4740 513
Size: 527 3217
Fragments arranged by size:
3217 939 800 759 552 527 306 300
23 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3919 487
Size: 683 56 2693 4012
Fragments arranged by size:
4012 2693 683 56
BtsI GCAGTG_nn'
Cuts at: 2878 3057 3380 3428 3928 3971 4475 4960 5754
Size: 179 323 48 500 43 504 485 794
Cuts at: 5754 5774 2878
Size: 20 4548
Fragments arranged by size:
4548 794 504 500 485 323 179 48
43 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2607 2642 529
Size: 35 2043 35 5331
Fragments arranged by size:
5331 2043 35 35
DraI TTT'AAA
Cuts at: 4748 4748
Size: 7444
DraIII CAC_nnn'GTG
Cuts at: 3778 3778
Size: 7444
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3989 4259 6451 809
Size: 507 2673 270 2192 1802
Fragments arranged by size:
2673 2192 1802 507 270
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2615 2760 3456
Size: 54 1425 50 275 18 785 145 696
Cuts at: 3456 3492 4098 4371 4564 4891 5780 7409 8
Size: 36 606 273 193 327 889 1629 43
Fragments arranged by size:
1629 1425 889 785 696 606 327 275
273 193 145 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 4891 1537
Size: 275 3079 4090
Fragments arranged by size:
4090 3079 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2320 4046 5374 1353
Size: 967 1726 1328 3423
Fragments arranged by size:
3423 1726 1328 967
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3322 4655 6031 6405 6551 387
Size: 1064 138 1733 1333 1376 374 146 1280
Fragments arranged by size:
1733 1376 1333 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2743 2743
Size: 7444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2300 3100 4598 5507 6104 6891
Size: 393 367 301 800 1498 909 597 787
Cuts at: 6891 7159 1239
Size: 268 1524
Fragments arranged by size:
1524 1498 909 800 787 597 393 367
301 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3851 3851
Size: 7444
EcoO109I rG'GnC_Cy
Cuts at: 2020 2488 3451 3602 4181 2020
Size: 468 963 151 579 5283
Fragments arranged by size:
5283 963 579 468 151
EcoRI G'AATT_C
Cuts at: 4753 5131 4753
Size: 378 7066
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2726 3644 2233
Size: 493 918 6033
Fragments arranged by size:
6033 918 493
FspI TGC'GCA
Cuts at: 2546 5950 2546
Size: 3404 4040
FspAI rTGC'GCAy
Cuts at: 2546 2546
Size: 7444
HaeII r_GCGC'y
Cuts at: 1678 2774 3322 3507 3766 6591 1678
Size: 1096 548 185 259 2825 2531
Fragments arranged by size:
2825 2531 1096 548 259 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2387 2419 2767 2799 2840
Size: 32 135 32 815 32 348 32 41
Cuts at: 2840 2872 3380 3412 3848 3875 3880 3907 6085
Size: 32 508 32 436 27 5 27 2178
Cuts at: 6085 6117 6159 6191 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2178 815 508 436 348 135 42
41 32 32 32 32 32 32 32
32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2665 4788 5034 669
Size: 988 281 727 2123 246 3079
Fragments arranged by size:
3079 2123 988 727 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7444
HpaI GTT'AAC
Cuts at: 5034 5034
Size: 7444
KasI G'GCGC_C
Cuts at: 3503 3503
Size: 7444
KpnI G_GTAC'C
Cuts at: 4769 4769
Size: 7444
MfeI C'AATT_G
Cuts at: 2497 5043 2497
Size: 2546 4898
MluI A'CGCG_T
Cuts at: 7320 7320
Size: 7444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2266 3586 6558 6742 1305
Size: 961 1320 2972 184 2007
Fragments arranged by size:
2972 2007 1320 961 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2762 3458 4373 10
Size: 54 1425 343 930 696 915 3081
Fragments arranged by size:
3081 1425 930 915 696 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2441 3062 3404 4463 5402 5761 5920
Size: 648 1275 621 342 1059 939 359 159
Cuts at: 5920 518
Size: 2042
Fragments arranged by size:
2042 1275 1059 939 648 621 359 342
159
MspA1I CmG'CkG
Cuts at: 1606 2287 2509 2731 3086 3591 3647 3678 4576
Size: 681 222 222 355 505 56 31 898
Cuts at: 4576 4687 4860 5537 6685 6930 1606
Size: 111 173 677 1148 245 2120
Fragments arranged by size:
2120 1148 898 681 677 505 355 245
222 222 173 111 56 31
NaeI GCC'GGC
Cuts at: 3492 3492
Size: 7444
NarI GG'CG_CC
Cuts at: 3504 3504
Size: 7444
NcoI C'CATG_G
Cuts at: 513 1065 2004 3063 513
Size: 552 939 1059 4894
Fragments arranged by size:
4894 1059 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7444
NgoMIV G'CCGG_C
Cuts at: 3490 3490
Size: 7444
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7444
NotI GC'GGCC_GC
Cuts at: 4891 4891
Size: 7444
NspI r_CATG'y
Cuts at: 1132 1229 3673 4468 4802 6347 1132
Size: 97 2444 795 334 1545 2229
Fragments arranged by size:
2444 2229 1545 795 334 97
PasI CC'CwG_GG
Cuts at: 1348 3529 1348
Size: 2181 5263
PciI A'CATG_T
Cuts at: 3669 6343 3669
Size: 2674 4770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2587 4675 1108
Size: 1479 2088 3877
Fragments arranged by size:
3877 2088 1479
PfoI T'CCnGG_A
Cuts at: 2277 3417 3439 4476 2277
Size: 1140 22 1037 5245
Fragments arranged by size:
5245 1140 1037 22
PmeI GTTT'AAAC
Cuts at: 4748 4748
Size: 7444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5513 5545 7026 7058 5513
Size: 32 1481 32 5899
Fragments arranged by size:
5899 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 3451 2020
Size: 1431 6013
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7444
PsiI TTA'TAA
Cuts at: 5014 5014
Size: 7444
PspOMI G'GGCC_C
Cuts at: 4523 4690 4523
Size: 167 7277
PspXI vC'TCGA_Gb
Cuts at: 2427 2866 3821 2427
Size: 439 955 6050
Fragments arranged by size:
6050 955 439
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2450 2482 4316 4348 2450
Size: 32 1834 32 5546
Fragments arranged by size:
5546 1834 32 32
PstI C_TGCA'G
Cuts at: 830 2139 2487 3182 4796 830
Size: 1309 348 695 1614 3478
Fragments arranged by size:
3478 1614 1309 695 348
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7444
PvuII CAG'CTG
Cuts at: 2731 3591 3678 4576 2731
Size: 860 87 898 5599
Fragments arranged by size:
5599 898 860 87
SacI G_AGCT'C
Cuts at: 2745 2745
Size: 7444
SacII CC_GC'GG
Cuts at: 3087 3087
Size: 7444
SalI G'TCGA_C
Cuts at: 1936 4786 1936
Size: 2850 4594
SapI GCTCTTCn'nnn_
Cuts at: 4046 4046
Size: 7444
SbfI CC_TGCA'GG
Cuts at: 2487 4796 2487
Size: 2309 5135
ScaI AGT'ACT
Cuts at: 3716 5692 7404 3716
Size: 1976 1712 3756
Fragments arranged by size:
3756 1976 1712
SexAI A'CCwGG_T
Cuts at: 4254 4254
Size: 7444
SfcI C'TryA_G
Cuts at: 826 1045 2135 2483 3178 4045 4792 5927 6608
Size: 219 1090 348 695 867 747 1135 681
Cuts at: 6608 6799 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1090 867 747 695 681 348
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4427 4427
Size: 7444
SfoI GGC'GCC
Cuts at: 3505 3505
Size: 7444
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7444
SmaI CCC'GGG
Cuts at: 1872 4522 4771 1872
Size: 2650 249 4545
Fragments arranged by size:
4545 2650 249
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2427 2744 2866 3269 3821 5548
Size: 197 926 484 317 122 403 552 1727
Cuts at: 5548 6449 6711 820
Size: 901 262 1553
Fragments arranged by size:
1727 1553 926 901 552 484 403 317
262 197 122
SnaBI TAC'GTA
Cuts at: 493 4036 5235 493
Size: 3543 1199 2702
Fragments arranged by size:
3543 2702 1199
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7444
SphI G_CATG'C
Cuts at: 1132 4468 4802 1132
Size: 3336 334 3774
Fragments arranged by size:
3774 3336 334
SspI AAT'ATT
Cuts at: 5 52 5368 5
Size: 47 5316 2081
Fragments arranged by size:
5316 2081 47
StuI AGG'CCT
Cuts at: 1517 2433 4421 1517
Size: 916 1988 4540
Fragments arranged by size:
4540 1988 916
StyI C'CwwG_G
Cuts at: 513 1065 2004 3063 3852 4883 513
Size: 552 939 1059 789 1031 3074
Fragments arranged by size:
3074 1059 1031 939 789 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2714 3883 5471 5488 5641 5826 6271
Size: 1255 1134 1169 1588 17 153 185 445
Cuts at: 6271 325
Size: 1498
Fragments arranged by size:
1588 1498 1255 1169 1134 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3555 3672 3714
Size: 274 80 33 51 1443 1578 117 42
Cuts at: 3714 3783 4293 5690 7402 96
Size: 69 510 1397 1712 138
Fragments arranged by size:
1712 1578 1443 1397 510 274 138 117
80 69 51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 7404 7436 2024
Size: 32 5348 32 2032
Fragments arranged by size:
5348 2032 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7444
XbaI T'CTAG_A
Cuts at: 4780 4780
Size: 7444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4351 4351
Size: 7444
XhoI C'TCGA_G
Cuts at: 1943 2427 2866 3821 1943
Size: 484 439 955 5566
Fragments arranged by size:
5566 955 484 439
XmaI C'CCGG_G
Cuts at: 1870 4520 4769 1870
Size: 2650 249 4545
Fragments arranged by size:
4545 2650 249
XmnI GAAnn'nnTTC
Cuts at: 5573 5573
Size: 7444
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5252 276
Size: 53 83 186 936 3718 2468
Fragments arranged by size:
3718 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BstZ17I
Bsu36I EcoRV FseI NruI NsiI PacI PmlI RsrII
SanDI SrfI SwaI