Restriction map for
pFN21AA1000
(Circular) (Six-base) MAPSORT of: pFN21AA1000.seq Check: 9813 from: 1 to: 10543
pFN21AA1000 10543 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6934 7039 6934
Size: 105 10438
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8353 278
Size: 53 83 186 936 6817 2468
Fragments arranged by size:
6817 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4087 7886 1937
Size: 2150 3799 4594
Fragments arranged by size:
4594 3799 2150
Acc65I G'GTAC_C
Cuts at: 3796 7864 3796
Size: 4068 6475
AclI AA'CG_TT
Cuts at: 1563 8670 9043 1563
Size: 7107 373 3063
Fragments arranged by size:
7107 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2393 3039 4287 4551 4616 4691 4697 5135
Size: 761 646 1248 264 65 75 6 438
Cuts at: 5135 5336 5357 6870 6902 6962 7061 7067 7284
Size: 201 21 1513 32 60 99 6 217
Cuts at: 7284 7367 7455 7637 8606 9990 10258 1632
Size: 83 88 182 969 1384 268 1917
Fragments arranged by size:
1917 1513 1384 1248 969 761 646 438
268 264 217 201 182 99 88 83
75 65 60 32 21 6 6
AfeI AGC'GCT
Cuts at: 5804 5804
Size: 10543
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10346
AflIII A'CryG_T
Cuts at: 1203 3516 9442 10419 1203
Size: 2313 5926 977 1327
Fragments arranged by size:
5926 2313 1327 977
AgeI A'CCGG_T
Cuts at: 9374 9374
Size: 10543
AhdI GACnn_n'nnGTC
Cuts at: 6325 9272 6325
Size: 2947 7596
AleI CACnn'nnGTG
Cuts at: 1166 2799 4325 1166
Size: 1633 1526 7384
Fragments arranged by size:
7384 1633 1526
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2817 4445 1184
Size: 1633 1628 7282
Fragments arranged by size:
7282 1633 1628
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3705 3737 3705
Size: 32 10511
AlwNI CAG_nnn'CTG
Cuts at: 2996 5059 5560 5689 8286 9858 2996
Size: 2063 501 129 2597 1572 3681
Fragments arranged by size:
3681 2597 2063 1572 501 129
ApaI G_GGCC'C
Cuts at: 3379 3379
Size: 10543
ApaLI G'TGCA_C
Cuts at: 4608 8600 9756 4608
Size: 3992 1156 5395
Fragments arranged by size:
5395 3992 1156
ApoI r'AATT_y
Cuts at: 1493 2780 2907 3128 3755 4439 4837 5311 5549
Size: 1287 127 221 627 684 398 474 238
Cuts at: 5549 6793 7852 8078 8230 10339 1493
Size: 1244 1059 226 152 2109 1697
Fragments arranged by size:
2109 1697 1287 1244 1059 684 627 474
398 238 226 221 152 127
AseI AT'TA_AT
Cuts at: 160 1032 3474 9097 160
Size: 872 2442 5623 1606
Fragments arranged by size:
5623 2442 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10543
AvaI C'yCGr_G
Cuts at: 1870 1943 5424 5648 6314 6335 7868 1870
Size: 73 3481 224 666 21 1533 4545
Fragments arranged by size:
4545 3481 1533 666 224 73 21
BamHI G'GATC_C
Cuts at: 5504 7873 8240 5504
Size: 2369 367 7807
Fragments arranged by size:
7807 2369 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2801 3314 3796 5147 6294
Size: 325 217 627 1014 513 482 1351 1147
Cuts at: 6294 7864 9319 618
Size: 1570 1455 1842
Fragments arranged by size:
1842 1570 1455 1351 1147 1014 627 513
482 325 217
BanII G_rGCy'C
Cuts at: 1391 3379 5649 6315 6483 6691 7158 7561 7862
Size: 1988 2270 666 168 208 467 403 301
Cuts at: 7862 1391
Size: 4072
Fragments arranged by size:
4072 2270 1988 666 467 403 301 208
168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 3867 4384 5363 5985 6198 6656
Size: 971 429 1539 517 979 622 213 458
Cuts at: 6656 7614 928
Size: 958 3857
Fragments arranged by size:
3857 1539 979 971 958 622 517 458
429 213
BbvCI CC'TCA_GC
Cuts at: 2202 5279 7211 2202
Size: 3077 1932 5534
Fragments arranged by size:
5534 3077 1932
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8734 8768 1903
Size: 34 6797 34 3678
Fragments arranged by size:
6797 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4684 8435 9645 4684
Size: 3751 1210 5582
Fragments arranged by size:
5582 3751 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10543
BfrBI ATG'CAT
Cuts at: 4056 7578 4056
Size: 3522 7021
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7310 7322 9154
Size: 107 122 71 631 605 5638 12 1832
Cuts at: 9154 136
Size: 1525
Fragments arranged by size:
5638 1832 1525 631 605 122 107 71
12
BglII A'GATC_T
Cuts at: 3604 10538 3604
Size: 6934 3609
BlpI GC'TnA_GC
Cuts at: 2118 4719 5090 5346 5517 5643 6048 6105 6926
Size: 2601 371 256 171 126 405 57 821
Cuts at: 6926 7062 7960 2118
Size: 136 898 4701
Fragments arranged by size:
4701 2601 898 821 405 371 256 171
136 126 57
Bme1580I G_kGCm'C
Cuts at: 1790 3319 3379 4612 5152 6025 8604 9760 1790
Size: 1529 60 1233 540 873 2579 1156 2573
Fragments arranged by size:
2579 2573 1529 1233 1156 873 540 60
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10543
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4297 8274 9232
Size: 416 53 83 399 24 2880 3977 958
Cuts at: 9232 442
Size: 1753
Fragments arranged by size:
3977 2880 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10543
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5471 5471
Size: 10543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2286 2898 3120 3548 3713 4805 5225 5510
Size: 1047 612 222 428 165 1092 420 285
Cuts at: 5510 6329 6905 7250 7538 9203 1239
Size: 819 576 345 288 1665 2579
Fragments arranged by size:
2579 1665 1092 1047 819 612 576 428
420 345 288 285 222 165
Bpu10I CC'TnA_GC
Cuts at: 2202 5099 5279 5763 7211 2202
Size: 2897 180 484 1448 5534
Fragments arranged by size:
5534 2897 1448 484 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3464 3479 5162 5174 6470 6476 7589 7770 8668
Size: 15 1683 12 1296 6 1113 181 898
Cuts at: 8668 9533 9831 3464
Size: 865 298 4176
Fragments arranged by size:
4176 1683 1296 1113 898 865 298 181
15 12 6
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2420 5999 9206 882
Size: 617 921 3579 3207 2219
Fragments arranged by size:
3579 3207 2219 921 617
BsaAI yAC'GTr
Cuts at: 493 1204 8334 493
Size: 711 7130 2702
Fragments arranged by size:
7130 2702 711
BsaBI GATnn'nnATC
Cuts at: 4433 6986 10537 4433
Size: 2553 3551 4439
Fragments arranged by size:
4439 3551 2553
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6321 8350 8732 9367
Size: 53 83 186 936 4788 2029 382 635
Cuts at: 9367 275
Size: 1451
Fragments arranged by size:
4788 2029 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6114 8976 9374 9648 9795 10206 6114
Size: 2862 398 274 147 411 6451
Fragments arranged by size:
6451 2862 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2963 2993 4569 4599 5875
Size: 30 165 30 1560 30 1576 30 1276
Cuts at: 5875 5905 1178
Size: 30 5816
Fragments arranged by size:
5816 1576 1560 1276 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2755 3205 4844 5093 5096 5507 6437 6473
Size: 1569 450 1639 249 3 411 930 36
Cuts at: 6473 6521 7199 1186
Size: 48 678 4530
Fragments arranged by size:
4530 1639 1569 930 678 450 411 249
48 36 3
BseYI C'CCAG_C
Cuts at: 4340 9746 10242 4340
Size: 5406 496 4641
Fragments arranged by size:
5406 4641 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5054 5057 5180 5546 5573 6095 7295
Size: 573 3318 3 123 366 27 522 1200
Cuts at: 7295 7560 1163
Size: 265 4146
Fragments arranged by size:
4146 3318 1200 573 522 366 265 123
27 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7993 8754 9782 1540
Size: 275 186 5992 761 1028 2301
Fragments arranged by size:
5992 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4417 4612 4959 5649 6315 6483 7158 8604 8689
Size: 195 347 690 666 168 675 1446 85
Cuts at: 8689 9760 4417
Size: 1071 5200
Fragments arranged by size:
5200 1446 1071 690 675 666 347 195
168 85
BsmI GAATG_Cn'
Cuts at: 2191 3414 4209 8054 8147 2191
Size: 1223 795 3845 93 4587
Fragments arranged by size:
4587 3845 1223 795 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3319 3379 4417 4612 4959 5152 5649
Size: 399 1529 60 1038 195 347 193 497
Cuts at: 5649 6025 6315 6483 6691 7158 7561 7862 8604
Size: 376 290 168 208 467 403 301 742
Cuts at: 8604 8689 9760 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1529 1071 1038 742 497 467 403
399 376 347 301 290 208 195 193
168 85 60
BspEI T'CCGG_A
Cuts at: 10206 10206
Size: 10543
BspHI T'CATG_A
Cuts at: 2932 8432 10162 2932
Size: 5500 1730 3313
Fragments arranged by size:
5500 3313 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2946 5076 5665 6934 7039 7898 844
Size: 2102 2130 589 1269 105 859 3489
Fragments arranged by size:
3489 2130 2102 1269 859 589 105
BsrBI CCG'CTC
Cuts at: 1394 1823 6348 7066 8430 1394
Size: 429 4525 718 1364 3507
Fragments arranged by size:
4525 3507 1364 718 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2337 6155 7365 9038 9212 65
Size: 1183 1089 3818 1210 1673 174 1396
Fragments arranged by size:
3818 1673 1396 1210 1183 1089 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6939 9187 9374 1700
Size: 110 5129 2248 187 2869
Fragments arranged by size:
5129 2869 2248 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10543
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10543
BssSI C'ACGA_G
Cuts at: 6419 8603 9615 6419
Size: 2184 1012 7347
Fragments arranged by size:
7347 2184 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6301 794
Size: 5507 5036
BstBI TT'CG_AA
Cuts at: 9347 9347
Size: 10543
BstEII G'GTnAC_C
Cuts at: 1184 3298 6871 1184
Size: 2114 3573 4856
Fragments arranged by size:
4856 3573 2114
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2876 6256 6967 2146
Size: 730 3380 711 5722
Fragments arranged by size:
5722 3380 730 711
BstYI r'GATC_y
Cuts at: 1972 2068 3604 4776 5504 7099 7873 8240 8625
Size: 96 1536 1172 728 1595 774 367 385
Cuts at: 8625 8642 10094 10180 10192 10538 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1595 1536 1452 1172 774 728 385
367 346 96 86 17 12
BstZ17I GTA'TAC
Cuts at: 4088 4088
Size: 10543
Bsu36I CC'TnA_GG
Cuts at: 3663 3663
Size: 10543
BtgI C'CryG_G
Cuts at: 513 1065 2004 3045 6249 513
Size: 552 939 1041 3204 4807
Fragments arranged by size:
4807 3204 1041 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7588 487
Size: 683 56 6362 3442
Fragments arranged by size:
6362 3442 683 56
BtsI GCAGTG_nn'
Cuts at: 4757 6818 7543 8059 8853 8873 4757
Size: 2061 725 516 794 20 6427
Fragments arranged by size:
6427 2061 794 725 516 20
ClaI AT'CG_AT
Cuts at: 1554 4902 1554
Size: 3348 7195
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10508
DraI TTT'AAA
Cuts at: 3133 4195 4336 5217 7847 3133
Size: 1062 141 881 2630 5829
Fragments arranged by size:
5829 2630 1062 881 141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9550 809
Size: 507 8234 1802
Fragments arranged by size:
8234 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2386 2475 3030
Size: 54 1425 50 275 18 556 89 555
Cuts at: 3030 6258 7132 7990 8879 10508 8
Size: 3228 874 858 889 1629 43
Fragments arranged by size:
3228 1629 1425 889 874 858 556 555
275 89 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7990 1537
Size: 275 6178 4090
Fragments arranged by size:
6178 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2423 2486 2588 3144 3642 4541 4596 4740
Size: 1070 63 102 556 498 899 55 144
Cuts at: 4740 4821 5268 5571 5615 5793 5910 6555 6639
Size: 81 447 303 44 178 117 645 84
Cuts at: 6639 6891 7083 7146 7260 7290 8473 1353
Size: 252 192 63 114 30 1183 3423
Fragments arranged by size:
3423 1183 1070 899 645 556 498 447
303 252 192 178 144 117 114 102
84 81 63 63 55 44 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4876 7709 9130 9504 9650 387
Size: 1064 138 3287 2833 1421 374 146 1280
Fragments arranged by size:
3287 2833 1421 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5647 6313 6481 7156 5647
Size: 666 168 675 9034
Fragments arranged by size:
9034 675 666 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2393 2898 3039 3120 3548 3713
Size: 393 654 107 505 141 81 428 165
Cuts at: 3713 4287 4551 4616 4691 4697 4805 5135 5225
Size: 574 264 65 75 6 108 330 90
Cuts at: 5225 5336 5357 5510 6329 6870 6902 6905 6962
Size: 111 21 153 819 541 32 3 57
Cuts at: 6962 7061 7067 7250 7284 7367 7455 7538 7637
Size: 99 6 183 34 83 88 83 99
Cuts at: 7637 8606 9203 9990 10258 1239
Size: 969 597 787 268 1524
Fragments arranged by size:
1524 969 819 787 654 597 574 541
505 428 393 330 268 264 183 165
153 141 111 108 107 99 99 90
88 83 83 81 75 65 57 34
32 21 6 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2348 4528 4682 5156 6041 6338 2348
Size: 2180 154 474 885 297 6553
Fragments arranged by size:
6553 2180 885 474 297 154
EcoO109I rG'GnC_Cy
Cuts at: 2034 3375 3376 4224 6394 6901 6994 7107 2034
Size: 1341 1 848 2170 507 93 113 5470
Fragments arranged by size:
5470 2170 1341 848 507 113 93 1
EcoRI G'AATT_C
Cuts at: 5311 7852 8230 5311
Size: 2541 378 7624
Fragments arranged by size:
7624 2541 378
EcoRV GAT'ATC
Cuts at: 2836 5005 2836
Size: 2169 8374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3857 4292 5725 5977 6770 3857
Size: 435 1433 252 793 7630
Fragments arranged by size:
7630 1433 793 435 252
FspI TGC'GCA
Cuts at: 9049 9049
Size: 10543
HaeII r_GCGC'y
Cuts at: 1678 2383 5806 9690 1678
Size: 705 3423 3884 2531
Fragments arranged by size:
3884 3423 2531 705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2284 2316 2750 2782 4006
Size: 32 135 32 712 32 434 32 1224
Cuts at: 4006 4038 4302 4334 4367 4399 4537 4569 5996
Size: 32 264 32 33 32 138 32 1427
Cuts at: 5996 6028 6311 6343 6556 6588 7091 7123 7621
Size: 32 283 32 213 32 503 32 498
Cuts at: 7621 7653 9184 9216 9258 9290 1373
Size: 32 1531 32 42 32 2626
Fragments arranged by size:
2626 1531 1427 1224 712 503 498 434
283 264 213 138 135 42 33 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 7887 8133 669
Size: 988 281 5949 246 3079
Fragments arranged by size:
5949 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3594 6764 748
Size: 2846 3170 4527
Fragments arranged by size:
4527 3170 2846
HpaI GTT'AAC
Cuts at: 8133 8133
Size: 10543
KpnI G_GTAC'C
Cuts at: 3800 7868 3800
Size: 4068 6475
MfeI C'AATT_G
Cuts at: 3508 8142 3508
Size: 4634 5909
MluI A'CGCG_T
Cuts at: 10419 10419
Size: 10543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3903 4788 9657 9841 1305
Size: 2598 885 4869 184 2007
Fragments arranged by size:
4869 2598 2007 885 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2388 3032 6260 10
Size: 54 1425 343 556 644 3228 4293
Fragments arranged by size:
4293 3228 1425 644 556 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2330 2346 2789 2799 4325 4508 6254
Size: 648 1164 16 443 10 1526 183 1746
Cuts at: 6254 8501 8860 9019 518
Size: 2247 359 159 2042
Fragments arranged by size:
2247 2042 1746 1526 1164 648 443 359
183 159 16 10
MspA1I CmG'CkG
Cuts at: 1606 4265 4783 5521 5995 6301 6307 6379 7009
Size: 2659 518 738 474 306 6 72 630
Cuts at: 7009 7959 8636 9784 10029 1606
Size: 950 677 1148 245 2120
Fragments arranged by size:
2659 2120 1148 950 738 677 630 518
474 306 245 72 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 3045 6249 513
Size: 552 939 1041 3204 4807
Fragments arranged by size:
4807 3204 1041 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10543
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10543
NotI GC'GGCC_GC
Cuts at: 7990 7990
Size: 10543
NsiI A_TGCA'T
Cuts at: 4058 7580 4058
Size: 3522 7021
NspI r_CATG'y
Cuts at: 1132 1229 2554 2815 3520 5630 7901 9446 1132
Size: 97 1325 261 705 2110 2271 1545 2229
Fragments arranged by size:
2271 2229 2110 1545 1325 705 261 97
PasI CC'CwG_GG
Cuts at: 1348 6967 1348
Size: 5619 4924
PciI A'CATG_T
Cuts at: 3516 9442 3516
Size: 5926 4617
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5467 1108
Size: 4359 6184
PfoI T'CCnGG_A
Cuts at: 2669 7158 2669
Size: 4489 6054
PmeI GTTT'AAAC
Cuts at: 7847 7847
Size: 10543
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8612 8644 10125 10157 8612
Size: 32 1481 32 8998
Fragments arranged by size:
8998 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4224 6901 6994 7107 4224
Size: 2677 93 113 7660
Fragments arranged by size:
7660 2677 113 93
PshAI GACnn'nnGTC
Cuts at: 1613 6656 1613
Size: 5043 5500
PsiI TTA'TAA
Cuts at: 3804 8113 3804
Size: 4309 6234
PspOMI G'GGCC_C
Cuts at: 3375 3375
Size: 10543
PspXI vC'TCGA_Gb
Cuts at: 5424 5648 5424
Size: 224 10319
PstI C_TGCA'G
Cuts at: 830 4182 6042 6306 6355 6384 6834 7895 830
Size: 3352 1860 264 49 29 450 1061 3478
Fragments arranged by size:
3478 3352 1860 1061 450 264 49 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10543
PvuII CAG'CTG
Cuts at: 4265 4783 5521 5995 6301 6307 6379 7009 4265
Size: 518 738 474 306 6 72 630 7799
Fragments arranged by size:
7799 738 630 518 474 306 72 6
SacI G_AGCT'C
Cuts at: 5649 6315 6483 7158 5649
Size: 666 168 675 9034
Fragments arranged by size:
9034 675 666 168
SalI G'TCGA_C
Cuts at: 1936 7885 1936
Size: 5949 4594
SapI GCTCTTCn'nnn_
Cuts at: 3144 4541 4740 5268 5793 7146 3144
Size: 1397 199 528 525 1353 6541
Fragments arranged by size:
6541 1397 1353 528 525 199
SbfI CC_TGCA'GG
Cuts at: 7895 7895
Size: 10543
ScaI AGT'ACT
Cuts at: 3919 8791 10503 3919
Size: 4872 1712 3959
Fragments arranged by size:
4872 3959 1712
SexAI A'CCwGG_T
Cuts at: 4081 6582 4081
Size: 2501 8042
SfcI C'TryA_G
Cuts at: 826 1045 2132 3288 3669 4109 4178 4950 5669
Size: 219 1087 1156 381 440 69 772 719
Cuts at: 5669 6038 6302 6351 6380 6830 7891 9026 9707
Size: 369 264 49 29 450 1061 1135 681
Cuts at: 9707 9898 826
Size: 191 1471
Fragments arranged by size:
1471 1156 1135 1087 1061 772 719 681
450 440 381 369 264 219 191 69
49 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10543
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10543
SmaI CCC'GGG
Cuts at: 1872 7870 1872
Size: 5998 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3443 3458 5141 5153 5424 5648
Size: 197 926 1500 15 1683 12 271 224
Cuts at: 5648 6485 6491 7568 7785 8647 9548 9810 820
Size: 837 6 1077 217 862 901 262 1553
Fragments arranged by size:
1683 1553 1500 1077 926 901 862 837
271 262 224 217 197 15 12 6
SnaBI TAC'GTA
Cuts at: 493 8334 493
Size: 7841 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10543
SphI G_CATG'C
Cuts at: 1132 2815 7901 1132
Size: 1683 5086 3774
Fragments arranged by size:
5086 3774 1683
SspI AAT'ATT
Cuts at: 5 52 8467 5
Size: 47 8415 2081
Fragments arranged by size:
8415 2081 47
StuI AGG'CCT
Cuts at: 1517 5337 1517
Size: 3820 6723
StyI C'CwwG_G
Cuts at: 513 1065 2004 2080 2145 3045 4220 4284 4626
Size: 552 939 76 65 900 1175 64 342
Cuts at: 4626 5028 6249 6279 7982 513
Size: 402 1221 30 1703 3074
Fragments arranged by size:
3074 1703 1221 1175 939 900 552 402
342 76 65 64 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3553 5474 8570 8587 8740 8925 9370
Size: 1255 1973 1921 3096 17 153 185 445
Cuts at: 9370 325
Size: 1498
Fragments arranged by size:
3096 1973 1921 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5786 6473
Size: 274 80 33 51 1443 1940 1869 687
Cuts at: 6473 8789 10501 96
Size: 2316 1712 138
Fragments arranged by size:
2316 1940 1869 1712 1443 687 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2598 2630 2963 2995 4141 4173 5875 5907 6410
Size: 32 333 32 1146 32 1702 32 503
Cuts at: 6410 6442 10503 10535 2598
Size: 32 4061 32 2606
Fragments arranged by size:
4061 2606 1702 1146 503 333 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10543
XbaI T'CTAG_A
Cuts at: 7879 7879
Size: 10543
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3052 3886 4310 4780 6214 7372 3052
Size: 834 424 470 1434 1158 6223
Fragments arranged by size:
6223 1434 1158 834 470 424
XhoI C'TCGA_G
Cuts at: 1943 5424 5648 1943
Size: 3481 224 6838
Fragments arranged by size:
6838 3481 224
XmaI C'CCGG_G
Cuts at: 1870 7868 1870
Size: 5998 4545
XmnI GAAnn'nnTTC
Cuts at: 4164 6766 8672 4164
Size: 2602 1906 6035
Fragments arranged by size:
6035 2602 1906
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8351 276
Size: 53 83 186 936 6817 2468
Fragments arranged by size:
6817 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BsiWI BsmBI DraIII FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI