Restriction map for
pFN21AA0987
(Circular) (Six-base) MAPSORT of: pFN21AA0987.seq Check: 1413 from: 1 to: 7966
pFN21AA0987 7966 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3660 4542 3660
Size: 882 7084
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5776 278
Size: 53 83 186 936 4240 2468
Fragments arranged by size:
4240 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5309 1937
Size: 3372 4594
Acc65I G'GTAC_C
Cuts at: 5287 5287
Size: 7966
AclI AA'CG_TT
Cuts at: 1563 3313 6093 6466 1563
Size: 1750 2780 373 3063
Fragments arranged by size:
3063 2780 1750 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2616 4368 4384 6029 7413 7681 1632
Size: 984 1752 16 1645 1384 268 1917
Fragments arranged by size:
1917 1752 1645 1384 984 268 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7769
AflIII A'CryG_T
Cuts at: 1203 5202 6865 7842 1203
Size: 3999 1663 977 1327
Fragments arranged by size:
3999 1663 1327 977
AgeI A'CCGG_T
Cuts at: 6797 6797
Size: 7966
AhdI GACnn_n'nnGTC
Cuts at: 2644 6695 2644
Size: 4051 3915
AleI CACnn'nnGTG
Cuts at: 1166 2174 3404 4602 1166
Size: 1008 1230 1198 4530
Fragments arranged by size:
4530 1230 1198 1008
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2192 4335 1184
Size: 1008 2143 4815
Fragments arranged by size:
4815 2143 1008
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4326 4358 4326
Size: 32 7934
AlwNI CAG_nnn'CTG
Cuts at: 2137 2582 4250 4766 5161 5709 7281 2137
Size: 445 1668 516 395 548 1572 2822
Fragments arranged by size:
2822 1668 1572 548 516 445 395
ApaLI G'TGCA_C
Cuts at: 4526 4595 5019 6023 7179 4526
Size: 69 424 1004 1156 5313
Fragments arranged by size:
5313 1156 1004 424 69
ApoI r'AATT_y
Cuts at: 1493 2564 3197 4433 4911 5275 5501 5653 7762
Size: 1071 633 1236 478 364 226 152 2109
Cuts at: 7762 1493
Size: 1697
Fragments arranged by size:
2109 1697 1236 1071 633 478 364 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6520 160
Size: 872 5488 1606
Fragments arranged by size:
5488 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7966
AvaI C'yCGr_G
Cuts at: 1870 1943 4048 5291 1870
Size: 73 2105 1243 4545
Fragments arranged by size:
4545 2105 1243 73
AvrII C'CTAG_G
Cuts at: 3700 3700
Size: 7966
BamHI G'GATC_C
Cuts at: 5296 5663 5296
Size: 367 7599
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 5287 6742 618
Size: 325 217 627 308 3192 1455 1842
Fragments arranged by size:
3192 1842 1455 627 325 308 217
BanII G_rGCy'C
Cuts at: 1391 2061 2693 2715 3588 3666 4011 4152 4688
Size: 670 632 22 873 78 345 141 536
Cuts at: 4688 5285 1391
Size: 597 4072
Fragments arranged by size:
4072 873 670 632 597 536 345 141
78 22
BbeI G_GCGC'C
Cuts at: 2099 2099
Size: 7966
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2259 2802 4356 4865 928
Size: 971 360 543 1554 509 4029
Fragments arranged by size:
4029 1554 971 543 509 360
BbvCI CC'TCA_GC
Cuts at: 3426 3426
Size: 7966
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4909 4943 6157 6191 1903
Size: 34 2972 34 1214 34 3678
Fragments arranged by size:
3678 2972 1214 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5858 7068 3809
Size: 2049 1210 4707
Fragments arranged by size:
4707 2049 1210
BclI T'GATC_A
Cuts at: 1548 3396 1548
Size: 1848 6118
BfrBI ATG'CAT
Cuts at: 2857 2857
Size: 7966
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6577 136
Size: 107 122 71 631 605 4905 1525
Fragments arranged by size:
4905 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4993 7961 4993
Size: 2968 4998
BlpI GC'TnA_GC
Cuts at: 3645 4541 5383 3645
Size: 896 842 6228
Fragments arranged by size:
6228 896 842
Bme1580I G_kGCm'C
Cuts at: 1790 3568 3906 4530 4599 5023 6027 7183 1790
Size: 1778 338 624 69 424 1004 1156 2573
Fragments arranged by size:
2573 1778 1156 1004 624 424 338 69
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7966
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5697 6655 442
Size: 416 53 83 399 24 4280 958 1753
Fragments arranged by size:
4280 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7966
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 7966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2377 3678 4025 5018 6626 1239
Size: 920 218 1301 347 993 1608 2579
Fragments arranged by size:
2579 1608 1301 993 920 347 218
Bpu10I CC'TnA_GC
Cuts at: 2508 2913 3426 5166 2508
Size: 405 513 1740 5308
Fragments arranged by size:
5308 1740 513 405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4045 4136 4161 6091 6956 7254 4045
Size: 91 25 1930 865 298 4757
Fragments arranged by size:
4757 1930 865 298 91 25
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2304 4103 4559 6629 882
Size: 617 805 1799 456 2070 2219
Fragments arranged by size:
2219 2070 1799 805 617 456
BsaAI yAC'GTr
Cuts at: 493 1204 2466 5757 493
Size: 711 1262 3291 2702
Fragments arranged by size:
3291 2702 1262 711
BsaBI GATnn'nnATC
Cuts at: 2904 7960 2904
Size: 5056 2910
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 2837 5773 6155
Size: 53 83 186 936 563 741 2936 382
Cuts at: 6155 6790 275
Size: 635 1451
Fragments arranged by size:
2936 1451 936 741 635 563 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2648 3412 6399 6797 7071 7218 7629 2648
Size: 764 2987 398 274 147 411 2985
Fragments arranged by size:
2987 2985 764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3394 3424 4556 4586 5250
Size: 30 165 30 1991 30 1132 30 664
Cuts at: 5250 5280 1178
Size: 30 3864
Fragments arranged by size:
3864 1991 1132 664 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 3443 3446 4595 4739 4802 4911 1186
Size: 949 1308 3 1149 144 63 109 4241
Fragments arranged by size:
4241 1308 1149 949 144 109 63 3
BseYI C'CCAG_C
Cuts at: 2686 3280 4467 7169 7665 2686
Size: 594 1187 2702 496 2987
Fragments arranged by size:
2987 2702 1187 594 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2798 4580 4818 1163
Size: 573 1062 1782 238 4311
Fragments arranged by size:
4311 1782 1062 573 238
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2980 3062 5416 6177 7205
Size: 275 186 12 967 82 2354 761 1028
Cuts at: 7205 1540
Size: 2301
Fragments arranged by size:
2354 2301 1028 967 761 275 186 82
12
BsiHKAI G_wGCw'C
Cuts at: 2715 3588 3651 4152 4530 4599 5023 6027 6112
Size: 873 63 501 378 69 424 1004 85
Cuts at: 6112 7183 2715
Size: 1071 3498
Fragments arranged by size:
3498 1071 1004 873 501 424 378 85
69 63
BsmI GAATG_Cn'
Cuts at: 4658 5477 5570 4658
Size: 819 93 7054
Fragments arranged by size:
7054 819 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4505 4505
Size: 7966
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2693 2715 3568 3588 3651 3666
Size: 399 271 632 22 853 20 63 15
Cuts at: 3666 3906 4011 4152 4530 4599 4688 5023 5285
Size: 240 105 141 378 69 89 335 262
Cuts at: 5285 6027 6112 7183 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 853 742 632 399 378 335
271 262 240 141 105 89 85 69
63 22 20 15
BspEI T'CCGG_A
Cuts at: 2648 3412 7629 2648
Size: 764 4217 2985
Fragments arranged by size:
4217 2985 764
BspHI T'CATG_A
Cuts at: 5855 7585 5855
Size: 1730 6236
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3660 4542 5321 844
Size: 2816 882 779 3489
Fragments arranged by size:
3489 2816 882 779
BsrBI CCG'CTC
Cuts at: 1394 1823 3436 3888 4585 5853 1394
Size: 429 1613 452 697 1268 3507
Fragments arranged by size:
3507 1613 1268 697 452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6461 6635 65
Size: 1183 5213 174 1396
Fragments arranged by size:
5213 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2979 6610 6797 1700
Size: 110 1169 3631 187 2869
Fragments arranged by size:
3631 2869 1169 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7966
BssHII G'CGCG_C
Cuts at: 1925 2086 1925
Size: 161 7805
BssSI C'ACGA_G
Cuts at: 3500 6026 7038 3500
Size: 2526 1012 4428
Fragments arranged by size:
4428 2526 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2337 4534 794
Size: 1543 2197 4226
Fragments arranged by size:
4226 2197 1543
BstBI TT'CG_AA
Cuts at: 2530 6770 2530
Size: 4240 3726
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7966
BstXI CCAn_nnnn'nTGG
Cuts at: 5221 5221
Size: 7966
BstYI r'GATC_y
Cuts at: 1972 3054 4993 5296 5663 6048 6065 7517 7603
Size: 1082 1939 303 367 385 17 1452 86
Cuts at: 7603 7615 7961 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1939 1452 1082 385 367 346 303
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7227
Fragments arranged by size:
7227 683 56
BtsI GCAGTG_nn'
Cuts at: 2342 4248 4888 5482 6276 6296 2342
Size: 1906 640 594 794 20 4012
Fragments arranged by size:
4012 1906 794 640 594 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7966
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7931
DraI TTT'AAA
Cuts at: 5270 5270
Size: 7966
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4360 6973 809
Size: 507 3044 2613 1802
Fragments arranged by size:
3044 2613 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3059 3355 3489
Size: 54 1425 50 275 18 1229 296 134
Cuts at: 3489 4302 5413 6302 7931 8
Size: 813 1111 889 1629 43
Fragments arranged by size:
1629 1425 1229 1111 889 813 296 275
134 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3059 5413 1537
Size: 275 1247 2354 4090
Fragments arranged by size:
4090 2354 1247 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3250 3441 3920 4062 4268 4344 4749 5896
Size: 1897 191 479 142 206 76 405 1147
Cuts at: 5896 1353
Size: 3423
Fragments arranged by size:
3423 1897 1147 479 405 206 191 142
76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6553 6927 7073 387
Size: 1064 138 4964 374 146 1280
Fragments arranged by size:
4964 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2713 3586 4150 2713
Size: 873 564 6529
Fragments arranged by size:
6529 873 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2377 2616 3678 4025 4368 4384
Size: 393 527 218 239 1062 347 343 16
Cuts at: 4384 5018 6029 6626 7413 7681 1239
Size: 634 1011 597 787 268 1524
Fragments arranged by size:
1524 1062 1011 787 634 597 527 393
347 343 268 239 218 16
EcoNI CCTnn'n_nnAGG
Cuts at: 3699 4748 3699
Size: 1049 6917
EcoO109I rG'GnC_Cy
Cuts at: 2134 2134
Size: 7966
EcoRI G'AATT_C
Cuts at: 4433 5275 5653 4433
Size: 842 378 6746
Fragments arranged by size:
6746 842 378
EcoRV GAT'ATC
Cuts at: 2602 2602
Size: 7966
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2366 2556 2366
Size: 190 7776
FspI TGC'GCA
Cuts at: 6472 6472
Size: 7966
HaeII r_GCGC'y
Cuts at: 1678 2099 4032 5162 7113 1678
Size: 421 1933 1130 1951 2531
Fragments arranged by size:
2531 1951 1933 1130 421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2625 2630 2657
Size: 32 135 32 446 32 575 5 27
Cuts at: 2657 2662 3494 3526 4180 4212 6607 6639 6681
Size: 5 832 32 654 32 2395 32 42
Cuts at: 6681 6713 1373
Size: 32 2626
Fragments arranged by size:
2626 2395 832 654 575 446 135 42
32 32 32 32 32 32 32 27
5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 5310 5556 669
Size: 988 281 3372 246 3079
Fragments arranged by size:
3372 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7966
HpaI GTT'AAC
Cuts at: 5556 5556
Size: 7966
KasI G'GCGC_C
Cuts at: 2095 2095
Size: 7966
KpnI G_GTAC'C
Cuts at: 5291 5291
Size: 7966
MfeI C'AATT_G
Cuts at: 5565 5565
Size: 7966
MluI A'CGCG_T
Cuts at: 7842 7842
Size: 7966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2478 4107 7080 7264 1305
Size: 1173 1629 2973 184 2007
Fragments arranged by size:
2973 2007 1629 1173 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3357 10
Size: 54 1425 343 1525 4619
Fragments arranged by size:
4619 1525 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 3404 4602 5207 5924 6283 6442
Size: 648 1008 1230 1198 605 717 359 159
Cuts at: 6442 518
Size: 2042
Fragments arranged by size:
2042 1230 1198 1008 717 648 605 359
159
MspA1I CmG'CkG
Cuts at: 1606 2156 4635 4766 5382 6059 7207 7452 1606
Size: 550 2479 131 616 677 1148 245 2120
Fragments arranged by size:
2479 2120 1148 677 616 550 245 131
NarI GG'CG_CC
Cuts at: 2096 2096
Size: 7966
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7414
NdeI CA'TA_TG
Cuts at: 387 4963 387
Size: 4576 3390
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7966
NotI GC'GGCC_GC
Cuts at: 5413 5413
Size: 7966
NruI TCG'CGA
Cuts at: 2976 2976
Size: 7966
NsiI A_TGCA'T
Cuts at: 2859 2859
Size: 7966
NspI r_CATG'y
Cuts at: 1132 1229 2335 5206 5324 6869 1132
Size: 97 1106 2871 118 1545 2229
Fragments arranged by size:
2871 2229 1545 1106 118 97
PasI CC'CwG_GG
Cuts at: 1348 3686 1348
Size: 2338 5628
PciI A'CATG_T
Cuts at: 5202 6865 5202
Size: 1663 6303
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5161 5255 1108
Size: 4053 94 3819
Fragments arranged by size:
4053 3819 94
PfoI T'CCnGG_A
Cuts at: 4743 4743
Size: 7966
PmeI GTTT'AAAC
Cuts at: 5270 5270
Size: 7966
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4042 4074 6035 6067 7548 7580 4042
Size: 32 1961 32 1481 32 4428
Fragments arranged by size:
4428 1961 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3534 1613
Size: 1921 6045
PsiI TTA'TAA
Cuts at: 5536 5536
Size: 7966
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 7934
PstI C_TGCA'G
Cuts at: 830 2161 4648 5318 830
Size: 1331 2487 670 3478
Fragments arranged by size:
3478 2487 1331 670
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7966
SacI G_AGCT'C
Cuts at: 2715 3588 4152 2715
Size: 873 564 6529
Fragments arranged by size:
6529 873 564
SalI G'TCGA_C
Cuts at: 1936 5308 1936
Size: 3372 4594
SapI GCTCTTCn'nnn_
Cuts at: 3250 3250
Size: 7966
SbfI CC_TGCA'GG
Cuts at: 5318 5318
Size: 7966
ScaI AGT'ACT
Cuts at: 6214 7926 6214
Size: 1712 6254
SexAI A'CCwGG_T
Cuts at: 2606 2606
Size: 7966
SfcI C'TryA_G
Cuts at: 826 1045 2157 3727 4644 5314 6449 7130 7321
Size: 219 1112 1570 917 670 1135 681 191
Cuts at: 7321 826
Size: 1471
Fragments arranged by size:
1570 1471 1135 1112 917 681 670 219
191
SfoI GGC'GCC
Cuts at: 2097 2097
Size: 7966
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7966
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7966
SmaI CCC'GGG
Cuts at: 1872 5293 1872
Size: 3421 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4024 4151 4176 6070 6971 7233
Size: 197 926 2081 127 25 1894 901 262
Cuts at: 7233 820
Size: 1553
Fragments arranged by size:
2081 1894 1553 926 901 262 197 127
25
SnaBI TAC'GTA
Cuts at: 493 2466 5757 493
Size: 1973 3291 2702
Fragments arranged by size:
3291 2702 1973
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7966
SphI G_CATG'C
Cuts at: 1132 2335 5324 1132
Size: 1203 2989 3774
Fragments arranged by size:
3774 2989 1203
SspI AAT'ATT
Cuts at: 5 52 5890 5
Size: 47 5838 2081
Fragments arranged by size:
5838 2081 47
StuI AGG'CCT
Cuts at: 1517 3694 1517
Size: 2177 5789
StyI C'CwwG_G
Cuts at: 513 1065 2115 3009 3207 3669 3700 4011 5405
Size: 552 1050 894 198 462 31 311 1394
Cuts at: 5405 513
Size: 3074
Fragments arranged by size:
3074 1394 1050 894 552 462 311 198
31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 2286 3572 5993 6010 6163 6348
Size: 1255 447 259 1286 2421 17 153 185
Cuts at: 6348 6793 325
Size: 445 1498
Fragments arranged by size:
2421 1498 1286 1255 447 445 259 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6212 7924 96
Size: 274 80 33 51 1443 4235 1712 138
Fragments arranged by size:
4235 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3127 3159 3394 3426 7926 7958 3127
Size: 32 235 32 4500 32 3135
Fragments arranged by size:
4500 3135 235 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4495 1316
Size: 3179 4787
XbaI T'CTAG_A
Cuts at: 5302 5302
Size: 7966
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7966
XmaI C'CCGG_G
Cuts at: 1870 5291 1870
Size: 3421 4545
XmnI GAAnn'nnTTC
Cuts at: 6095 6095
Size: 7966
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5774 276
Size: 53 83 186 936 4240 2468
Fragments arranged by size:
4240 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BsiWI BstZ17I Bsu36I DraIII
FseI FspAI NaeI NgoMIV PacI PmlI PpuMI PspOMI
PspXI PvuII RsrII SacII SanDI SfiI SrfI SwaI
XcmI