Restriction map for
pFN21AA0985
(Circular) (Six-base) MAPSORT of: pFN21AA0985.seq Check: 5086 from: 1 to: 6787
pFN21AA0985 6787 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3616 3616
Size: 6787
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4597 278
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4130 1937
Size: 2193 4594
Acc65I G'GTAC_C
Cuts at: 4108 4108
Size: 6787
AclI AA'CG_TT
Cuts at: 1563 4914 5287 1563
Size: 3351 373 3063
Fragments arranged by size:
3351 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3281 3536 4850 6234 6502 1632
Size: 1649 255 1314 1384 268 1917
Fragments arranged by size:
1917 1649 1384 1314 268 255
AfeI AGC'GCT
Cuts at: 3995 4046 3995
Size: 51 6736
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6590
AflIII A'CryG_T
Cuts at: 1203 4074 5686 6663 1203
Size: 2871 1612 977 1327
Fragments arranged by size:
2871 1612 1327 977
AgeI A'CCGG_T
Cuts at: 5618 5618
Size: 6787
AhdI GACnn_n'nnGTC
Cuts at: 5516 5516
Size: 6787
AleI CACnn'nnGTG
Cuts at: 1166 2115 1166
Size: 949 5838
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3753 1184
Size: 2569 4218
AlwNI CAG_nnn'CTG
Cuts at: 2657 2669 4530 6102 2657
Size: 12 1861 1572 3342
Fragments arranged by size:
3342 1861 1572 12
ApaI G_GGCC'C
Cuts at: 2639 2639
Size: 6787
ApaLI G'TGCA_C
Cuts at: 3235 3728 4844 6000 3235
Size: 493 1116 1156 4022
Fragments arranged by size:
4022 1156 1116 493
ApoI r'AATT_y
Cuts at: 1493 3194 3464 3479 3535 3851 4096 4322 4474
Size: 1701 270 15 56 316 245 226 152
Cuts at: 4474 6583 1493
Size: 2109 1697
Fragments arranged by size:
2109 1701 1697 316 270 245 226 152
56 15
AseI AT'TA_AT
Cuts at: 160 1032 5341 160
Size: 872 4309 1606
Fragments arranged by size:
4309 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
AvaI C'yCGr_G
Cuts at: 1870 1943 2193 2598 2610 2638 2675 2988 3597
Size: 73 250 405 12 28 37 313 609
Cuts at: 3597 4112 1870
Size: 515 4545
Fragments arranged by size:
4545 609 515 405 313 250 73 37
28 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4000 4033 4000
Size: 33 6754
BamHI G'GATC_C
Cuts at: 4117 4484 4117
Size: 367 6420
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3185 4050 4108 5563 618
Size: 325 217 627 1398 865 58 1455 1842
Fragments arranged by size:
1842 1455 1398 865 627 325 217 58
BanII G_rGCy'C
Cuts at: 1391 2333 2586 2605 2639 2732 2795 2861 4106
Size: 942 253 19 34 93 63 66 1245
Cuts at: 4106 1391
Size: 4072
Fragments arranged by size:
4072 1245 942 253 93 66 63 34
19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2531 2654 3437 928
Size: 971 632 123 783 4278
Fragments arranged by size:
4278 971 783 632 123
BbvCI CC'TCA_GC
Cuts at: 2871 2871
Size: 6787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4978 5012 1903
Size: 34 3041 34 3678
Fragments arranged by size:
3678 3041 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4679 5889 4679
Size: 1210 5577
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6787
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2131 2717 5398
Size: 107 122 71 631 605 459 586 2681
Cuts at: 5398 136
Size: 1525
Fragments arranged by size:
2681 1525 631 605 586 459 122 107
71
BglII A'GATC_T
Cuts at: 6782 6782
Size: 6787
BlpI GC'TnA_GC
Cuts at: 4204 4204
Size: 6787
Bme1580I G_kGCm'C
Cuts at: 1790 2639 3190 3239 3732 4848 6004 1790
Size: 849 551 49 493 1116 1156 2573
Fragments arranged by size:
2573 1156 1116 849 551 493 49
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6787
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2046 4518 5476
Size: 416 53 83 399 24 629 2472 958
Cuts at: 5476 442
Size: 1753
Fragments arranged by size:
2472 1753 958 629 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2298 2484 2589 2767 2796 2815 3572
Size: 836 223 186 105 178 29 19 757
Cuts at: 3572 5447 1239
Size: 1875 2579
Fragments arranged by size:
2579 1875 836 757 223 186 178 105
29 19
Bpu10I CC'TnA_GC
Cuts at: 2580 2871 2580
Size: 291 6496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3491 4912 5777 6075 3491
Size: 1421 865 298 4203
Fragments arranged by size:
4203 1421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2384 3380 3485 3676 5450 882
Size: 617 885 996 105 191 1774 2219
Fragments arranged by size:
2219 1774 996 885 617 191 105
BsaAI yAC'GTr
Cuts at: 493 1204 4075 4578 493
Size: 711 2871 503 2702
Fragments arranged by size:
2871 2702 711 503
BsaBI GATnn'nnATC
Cuts at: 6781 6781
Size: 6787
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2236 4594 4976 5611
Size: 53 83 186 936 703 2358 382 635
Cuts at: 5611 275
Size: 1451
Fragments arranged by size:
2358 1451 936 703 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3062 3790 5220 5618 5892 6039 6450 3062
Size: 728 1430 398 274 147 411 3399
Fragments arranged by size:
3399 1430 728 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2271 2301 2484 2514 3209
Size: 30 165 30 868 30 183 30 695
Cuts at: 3209 3239 1178
Size: 30 4726
Fragments arranged by size:
4726 868 695 183 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 3008 3146 3149 3665 3875 1186
Size: 991 831 138 3 516 210 4098
Fragments arranged by size:
4098 991 831 516 210 138 3
BseYI C'CCAG_C
Cuts at: 2317 2326 2535 2662 2754 2794 2860 3022 3096
Size: 9 209 127 92 40 66 162 74
Cuts at: 3096 3578 3970 5990 6486 2317
Size: 482 392 2020 496 2618
Fragments arranged by size:
2618 2020 496 482 392 209 162 127
92 74 66 40 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2356 2873 3290 1163
Size: 573 620 517 417 4660
Fragments arranged by size:
4660 620 573 517 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4237 4998 6026 1540
Size: 275 186 2236 761 1028 2301
Fragments arranged by size:
2301 2236 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2732 3239 3732 4848 4933 6004 2732
Size: 507 493 1116 85 1071 3515
Fragments arranged by size:
3515 1116 1071 507 493 85
BsmI GAATG_Cn'
Cuts at: 2407 4298 4391 2407
Size: 1891 93 4803
Fragments arranged by size:
4803 1891 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2800 2800
Size: 6787
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2586 2605 2639 2732 2795 2861
Size: 399 543 253 19 34 93 63 66
Cuts at: 2861 3190 3239 3732 4106 4848 4933 6004 1391
Size: 329 49 493 374 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 543 493 399 374 329
253 93 85 66 63 49 34 19
BspEI T'CCGG_A
Cuts at: 3062 6450 3062
Size: 3388 3399
BspHI T'CATG_A
Cuts at: 4676 6406 4676
Size: 1730 5057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2789 2845 3616 4142 844
Size: 1945 56 771 526 3489
Fragments arranged by size:
3489 1945 771 526 56
BsrBI CCG'CTC
Cuts at: 1394 1823 4674 1394
Size: 429 2851 3507
Fragments arranged by size:
3507 2851 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5282 5456 65
Size: 1183 4034 174 1396
Fragments arranged by size:
4034 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2058 5431 5618 1700
Size: 110 248 3373 187 2869
Fragments arranged by size:
3373 2869 248 187 110
BsrGI T'GTAC_A
Cuts at: 96 3681 96
Size: 3585 3202
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6787
BssSI C'ACGA_G
Cuts at: 3392 4847 5859 3392
Size: 1455 1012 4320
Fragments arranged by size:
4320 1455 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2379 2430 3313 794
Size: 1585 51 883 4268
Fragments arranged by size:
4268 1585 883 51
BstBI TT'CG_AA
Cuts at: 5591 5591
Size: 6787
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6787
BstXI CCAn_nnnn'nTGG
Cuts at: 2117 2117
Size: 6787
BstYI r'GATC_y
Cuts at: 1972 3441 3663 3975 4117 4484 4869 4886 6338
Size: 1469 222 312 142 367 385 17 1452
Cuts at: 6338 6424 6436 6782 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1469 1452 385 367 346 312 222
142 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3437 3437
Size: 6787
BtgI C'CryG_G
Cuts at: 513 1065 3267 3741 513
Size: 552 2202 474 3559
Fragments arranged by size:
3559 2202 552 474
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6048
Fragments arranged by size:
6048 683 56
BtsI GCAGTG_nn'
Cuts at: 2976 3239 4303 5097 5117 2976
Size: 263 1064 794 20 4646
Fragments arranged by size:
4646 1064 794 263 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6787
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6752
DraI TTT'AAA
Cuts at: 4091 4091
Size: 6787
DraIII CAC_nnn'GTG
Cuts at: 2379 2379
Size: 6787
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3589 3770 3917 5794 809
Size: 507 2273 181 147 1877 1802
Fragments arranged by size:
2273 1877 1802 507 181 147
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3469 4234 5123
Size: 54 1425 50 275 18 1639 765 889
Cuts at: 5123 6752 8
Size: 1629 43
Fragments arranged by size:
1639 1629 1425 889 765 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4234 1537
Size: 275 2422 4090
Fragments arranged by size:
4090 2422 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 2205 3135 3615 4717 1353
Size: 789 63 930 480 1102 3423
Fragments arranged by size:
3423 1102 930 789 480 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5374 5748 5894 387
Size: 1064 138 3785 374 146 1280
Fragments arranged by size:
3785 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2730 2730
Size: 6787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2298 2484 2589 2767 2796 2815
Size: 393 443 223 186 105 178 29 19
Cuts at: 2815 3281 3536 3572 4850 5447 6234 6502 1239
Size: 466 255 36 1278 597 787 268 1524
Fragments arranged by size:
1524 1278 787 597 466 443 393 268
255 223 186 178 105 36 29 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2841 3614 2841
Size: 773 6014
EcoO109I rG'GnC_Cy
Cuts at: 2497 2635 2654 2709 2889 2912 3256 2497
Size: 138 19 55 180 23 344 6028
Fragments arranged by size:
6028 344 180 138 55 23 19
EcoRI G'AATT_C
Cuts at: 3194 3851 4096 4474 3194
Size: 657 245 378 5507
Fragments arranged by size:
5507 657 378 245
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3856 3856
Size: 6787
FspI TGC'GCA
Cuts at: 2705 5293 2705
Size: 2588 4199
HaeII r_GCGC'y
Cuts at: 1678 2867 3997 4048 5934 1678
Size: 1189 1130 51 1886 2531
Fragments arranged by size:
2531 1886 1189 1130 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 3209 3241 3489
Size: 32 135 32 1079 32 526 32 248
Cuts at: 3489 3521 5428 5460 5502 5534 1373
Size: 32 1907 32 42 32 2626
Fragments arranged by size:
2626 1907 1079 526 248 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4131 4377 669
Size: 988 281 2193 246 3079
Fragments arranged by size:
3079 2193 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3338 748
Size: 2590 4197
HpaI GTT'AAC
Cuts at: 4377 4377
Size: 6787
KpnI G_GTAC'C
Cuts at: 4112 4112
Size: 6787
MfeI C'AATT_G
Cuts at: 4386 4386
Size: 6787
MluI A'CGCG_T
Cuts at: 6663 6663
Size: 6787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2021 3010 3357 5901 6085 1305
Size: 716 989 347 2544 184 2007
Fragments arranged by size:
2544 2007 989 716 347 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3471 10
Size: 54 1425 343 1639 3326
Fragments arranged by size:
3326 1639 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2115 4745 5104 5263 518
Size: 648 949 2630 359 159 2042
Fragments arranged by size:
2630 2042 949 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2295 2317 3089 3970 4203 4880 6028 6273
Size: 689 22 772 881 233 677 1148 245
Cuts at: 6273 1606
Size: 2120
Fragments arranged by size:
2120 1148 881 772 689 677 245 233
22
NcoI C'CATG_G
Cuts at: 513 1065 3267 3741 513
Size: 552 2202 474 3559
Fragments arranged by size:
3559 2202 552 474
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6787
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6787
NotI GC'GGCC_GC
Cuts at: 4234 4234
Size: 6787
NspI r_CATG'y
Cuts at: 1132 1229 3424 4145 5690 1132
Size: 97 2195 721 1545 2229
Fragments arranged by size:
2229 2195 1545 721 97
PasI CC'CwG_GG
Cuts at: 1348 2969 3181 1348
Size: 1621 212 4954
Fragments arranged by size:
4954 1621 212
PciI A'CATG_T
Cuts at: 5686 5686
Size: 6787
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3029 1108
Size: 1921 4866
PmeI GTTT'AAAC
Cuts at: 4091 4091
Size: 6787
PmlI CAC'GTG
Cuts at: 4075 4075
Size: 6787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4856 4888 6369 6401 4856
Size: 32 1481 32 5242
Fragments arranged by size:
5242 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2497 2889 2912 2497
Size: 392 23 6372
Fragments arranged by size:
6372 392 23
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6787
PsiI TTA'TAA
Cuts at: 4357 4357
Size: 6787
PspOMI G'GGCC_C
Cuts at: 2635 2635
Size: 6787
PspXI vC'TCGA_Gb
Cuts at: 2193 2610 2193
Size: 417 6370
PstI C_TGCA'G
Cuts at: 830 3088 3109 3234 4139 830
Size: 2258 21 125 905 3478
Fragments arranged by size:
3478 2258 905 125 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6787
PvuII CAG'CTG
Cuts at: 2317 3089 3970 2317
Size: 772 881 5134
Fragments arranged by size:
5134 881 772
SacI G_AGCT'C
Cuts at: 2732 2732
Size: 6787
SalI G'TCGA_C
Cuts at: 1936 4129 1936
Size: 2193 4594
SanDI GG'GwC_CC
Cuts at: 2889 2889
Size: 6787
SapI GCTCTTCn'nnn_
Cuts at: 2142 3615 2142
Size: 1473 5314
SbfI CC_TGCA'GG
Cuts at: 4139 4139
Size: 6787
ScaI AGT'ACT
Cuts at: 5035 6747 5035
Size: 1712 5075
SfcI C'TryA_G
Cuts at: 826 1045 3084 3105 3230 4058 4135 5270 5951
Size: 219 2039 21 125 828 77 1135 681
Cuts at: 5951 6142 826
Size: 191 1471
Fragments arranged by size:
2039 1471 1135 828 681 219 191 125
77 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6787
SmaI CCC'GGG
Cuts at: 1872 2600 2640 2677 4114 1872
Size: 728 40 37 1437 4545
Fragments arranged by size:
4545 1437 728 40 37
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2193 2610 3506 4891 5792 6054
Size: 197 926 250 417 896 1385 901 262
Cuts at: 6054 820
Size: 1553
Fragments arranged by size:
1553 1385 926 901 896 417 262 250
197
SnaBI TAC'GTA
Cuts at: 493 4578 493
Size: 4085 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6787
SphI G_CATG'C
Cuts at: 1132 4145 1132
Size: 3013 3774
SrfI GCCC'GGGC
Cuts at: 2600 2600
Size: 6787
SspI AAT'ATT
Cuts at: 5 52 4711 5
Size: 47 4659 2081
Fragments arranged by size:
4659 2081 47
StuI AGG'CCT
Cuts at: 1517 2828 3702 1517
Size: 1311 874 4602
Fragments arranged by size:
4602 1311 874
StyI C'CwwG_G
Cuts at: 513 1065 3252 3267 3741 3984 4226 513
Size: 552 2187 15 474 243 242 3074
Fragments arranged by size:
3074 2187 552 474 243 242 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3060 3173 4814 4831 4984 5169 5614
Size: 1255 1480 113 1641 17 153 185 445
Cuts at: 5614 325
Size: 1498
Fragments arranged by size:
1641 1498 1480 1255 445 185 153 113
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3681 5033 6745
Size: 274 80 33 51 1443 1704 1352 1712
Cuts at: 6745 96
Size: 138
Fragments arranged by size:
1712 1704 1443 1352 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2226 2258 2271 2303 6747 6779 2226
Size: 32 13 32 4444 32 2234
Fragments arranged by size:
4444 2234 32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 1316 2910 1316
Size: 1594 5193
XbaI T'CTAG_A
Cuts at: 4123 4123
Size: 6787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2383 2383
Size: 6787
XhoI C'TCGA_G
Cuts at: 1943 2193 2610 1943
Size: 250 417 6120
Fragments arranged by size:
6120 417 250
XmaI C'CCGG_G
Cuts at: 1870 2598 2638 2675 4112 1870
Size: 728 40 37 1437 4545
Fragments arranged by size:
4545 1437 728 40 37
XmnI GAAnn'nnTTC
Cuts at: 3536 4916 3536
Size: 1380 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4595 276
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PsrI RsrII SacII SexAI SfiI
SfoI SwaI