Restriction map for
pFN21AA0984
(Circular) (Six-base) MAPSORT of: pFN21AA0984.seq Check: 930 from: 1 to: 6646
pFN21AA0984 6646 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4456 278
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3989 1937
Size: 2052 4594
Acc65I G'GTAC_C
Cuts at: 3967 3967
Size: 6646
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2326 2381 3113 3558 3889 4709 6093 6361
Size: 694 55 732 445 331 820 1384 268
Cuts at: 6361 1632
Size: 1917
Fragments arranged by size:
1917 1384 820 732 694 445 331 268
55
AflII C'TTAA_G
Cuts at: 820 1017 3332 820
Size: 197 2315 4134
Fragments arranged by size:
4134 2315 197
AflIII A'CryG_T
Cuts at: 1203 5545 6522 1203
Size: 4342 977 1327
Fragments arranged by size:
4342 1327 977
AgeI A'CCGG_T
Cuts at: 5477 5477
Size: 6646
AhdI GACnn_n'nnGTC
Cuts at: 3402 5375 3402
Size: 1973 4673
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6646
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 6614
AlwNI CAG_nnn'CTG
Cuts at: 2386 2810 2974 4389 5961 2386
Size: 424 164 1415 1572 3071
Fragments arranged by size:
3071 1572 1415 424 164
ApaLI G'TGCA_C
Cuts at: 3683 3723 3878 4703 5859 3683
Size: 40 155 825 1156 4470
Fragments arranged by size:
4470 1156 825 155 40
ApoI r'AATT_y
Cuts at: 1493 2055 2624 3955 4181 4333 6442 1493
Size: 562 569 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 569 562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5200 160
Size: 872 4168 1606
Fragments arranged by size:
4168 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 3971 1870
Size: 73 2028 4545
Fragments arranged by size:
4545 2028 73
BamHI G'GATC_C
Cuts at: 3976 4343 3976
Size: 367 6279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2643 3967 5422 618
Size: 325 217 627 856 1324 1455 1842
Fragments arranged by size:
1842 1455 1324 856 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2439 3637 3678 3965 1391
Size: 1048 1198 41 287 4072
Fragments arranged by size:
4072 1198 1048 287 41
BbeI G_GCGC'C
Cuts at: 2647 2647
Size: 6646
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2332 2446 2524 3333 928
Size: 971 433 114 78 809 4241
Fragments arranged by size:
4241 971 809 433 114 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2849 2883 3089 3123 4837
Size: 34 50 34 828 34 206 34 1714
Cuts at: 4837 4871 1903
Size: 34 3678
Fragments arranged by size:
3678 1714 828 206 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2383 4538 5748 2383
Size: 2155 1210 3281
Fragments arranged by size:
3281 2155 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6646
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3540 5257 136
Size: 107 122 71 631 605 1868 1717 1525
Fragments arranged by size:
1868 1717 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2517 2978 6641 2517
Size: 461 3663 2522
Fragments arranged by size:
3663 2522 461
BlpI GC'TnA_GC
Cuts at: 2115 4063 2115
Size: 1948 4698
Bme1580I G_kGCm'C
Cuts at: 1790 3687 3727 3882 4707 5863 1790
Size: 1897 40 155 825 1156 2573
Fragments arranged by size:
2573 1897 1156 825 155 40
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6646
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4377 5335 442
Size: 416 53 83 399 24 2960 958 1753
Fragments arranged by size:
2960 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3192 5306 1239
Size: 1953 2114 2579
Fragments arranged by size:
2579 2114 1953
Bpu10I CC'TnA_GC
Cuts at: 3677 3677
Size: 6646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4771 5636 5934 4771
Size: 865 298 5483
Fragments arranged by size:
5483 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2340 5309 882
Size: 617 841 2969 2219
Fragments arranged by size:
2969 2219 841 617
BsaAI yAC'GTr
Cuts at: 493 1204 4437 493
Size: 711 3233 2702
Fragments arranged by size:
3233 2702 711
BsaBI GATnn'nnATC
Cuts at: 6640 6640
Size: 6646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2644 4453 4835 5470
Size: 53 83 186 936 1111 1809 382 635
Cuts at: 5470 275
Size: 1451
Fragments arranged by size:
1809 1451 1111 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3574 5079 5477 5751 5898 6309 3574
Size: 1505 398 274 147 411 3911
Fragments arranged by size:
3911 1505 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3569 3599 1178
Size: 30 165 30 2166 30 4225
Fragments arranged by size:
4225 2166 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2978 1186
Size: 1792 4854
BseYI C'CCAG_C
Cuts at: 2782 3472 3515 3800 5849 6345 2782
Size: 690 43 285 2049 496 3083
Fragments arranged by size:
3083 2049 690 496 285 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2193 1163
Size: 573 457 5616
Fragments arranged by size:
5616 573 457
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3561 4096 4857 5885 1540
Size: 275 186 1560 535 761 1028 2301
Fragments arranged by size:
2301 1560 1028 761 535 275 186
BsiHKAI G_wGCw'C
Cuts at: 3678 3687 3727 3882 4707 4792 5863 3678
Size: 9 40 155 825 85 1071 4461
Fragments arranged by size:
4461 1071 825 155 85 40 9
BsmI GAATG_Cn'
Cuts at: 3180 3274 3488 4157 4250 3180
Size: 94 214 669 93 5576
Fragments arranged by size:
5576 669 214 94 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3195 3195
Size: 6646
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 3637 3678 3687 3727 3882 3965
Size: 399 649 1198 41 9 40 155 83
Cuts at: 3965 4707 4792 5863 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1198 1071 742 649 399 155 85
83 41 40 9
BspEI T'CCGG_A
Cuts at: 3574 6309 3574
Size: 2735 3911
BspHI T'CATG_A
Cuts at: 4535 6265 4535
Size: 1730 4916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3124 4001 844
Size: 2280 877 3489
Fragments arranged by size:
3489 2280 877
BsrBI CCG'CTC
Cuts at: 1394 1823 4533 1394
Size: 429 2710 3507
Fragments arranged by size:
3507 2710 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2242 2988 5141 5315 65
Size: 1183 994 746 2153 174 1396
Fragments arranged by size:
2153 1396 1183 994 746 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3580 5290 5477 1700
Size: 110 1770 1710 187 2869
Fragments arranged by size:
2869 1770 1710 187 110
BsrGI T'GTAC_A
Cuts at: 96 3147 3503 96
Size: 3051 356 3239
Fragments arranged by size:
3239 3051 356
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 4706 5718 4706
Size: 1012 5634
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6646
BstBI TT'CG_AA
Cuts at: 5450 5450
Size: 6646
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6646
BstXI CCAn_nnnn'nTGG
Cuts at: 3287 3472 3841 3287
Size: 185 369 6092
Fragments arranged by size:
6092 369 185
BstYI r'GATC_y
Cuts at: 1972 2517 2978 3976 4343 4728 4745 6197 6283
Size: 545 461 998 367 385 17 1452 86
Cuts at: 6283 6295 6641 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 998 545 461 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2558 2558
Size: 6646
BtgI C'CryG_G
Cuts at: 513 1065 2049 2637 2712 3353 513
Size: 552 984 588 75 641 3806
Fragments arranged by size:
3806 984 641 588 552 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5907
Fragments arranged by size:
5907 683 56
BtsI GCAGTG_nn'
Cuts at: 2471 2889 3703 4162 4956 4976 2471
Size: 418 814 459 794 20 4141
Fragments arranged by size:
4141 814 794 459 418 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2401 2436 529
Size: 35 1837 35 4739
Fragments arranged by size:
4739 1837 35 35
DraI TTT'AAA
Cuts at: 2514 3950 2514
Size: 1436 5210
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5653 809
Size: 507 4337 1802
Fragments arranged by size:
4337 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 4093 4982
Size: 54 1425 50 275 18 274 1989 889
Cuts at: 4982 6611 8
Size: 1629 43
Fragments arranged by size:
1989 1629 1425 889 275 274 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4093 1537
Size: 275 2281 4090
Fragments arranged by size:
4090 2281 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2400 2838 4576 1353
Size: 1047 438 1738 3423
Fragments arranged by size:
3423 1738 1047 438
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5233 5607 5753 387
Size: 1064 138 3644 374 146 1280
Fragments arranged by size:
3644 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3676 3676
Size: 6646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2326 2381 3113 3192 3558 3889 4709
Size: 393 694 55 732 79 366 331 820
Cuts at: 4709 5306 6093 6361 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 820 787 732 694 597 393 366
331 268 79 55
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2651
Size: 6646
EcoO109I rG'GnC_Cy
Cuts at: 2071 2071
Size: 6646
EcoRI G'AATT_C
Cuts at: 2055 2624 3955 4333 2055
Size: 569 1331 378 4368
Fragments arranged by size:
4368 1331 569 378
EcoRV GAT'ATC
Cuts at: 3082 3082
Size: 6646
FspI TGC'GCA
Cuts at: 5152 5152
Size: 6646
HaeII r_GCGC'y
Cuts at: 1678 2647 5793 1678
Size: 969 3146 2531
Fragments arranged by size:
3146 2531 969
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 3388 3420 3554
Size: 32 135 32 458 32 1326 32 134
Cuts at: 3554 3586 5287 5319 5361 5393 1373
Size: 32 1701 32 42 32 2626
Fragments arranged by size:
2626 1701 1326 458 135 134 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3990 4236 669
Size: 988 281 2052 246 3079
Fragments arranged by size:
3079 2052 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6646
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KasI G'GCGC_C
Cuts at: 2643 2643
Size: 6646
KpnI G_GTAC'C
Cuts at: 3971 3971
Size: 6646
MfeI C'AATT_G
Cuts at: 2735 4245 2735
Size: 1510 5136
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5760 5944 1305
Size: 4455 184 2007
Fragments arranged by size:
4455 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 10
Size: 54 1425 343 274 4550
Fragments arranged by size:
4550 1425 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4604 4963 5122 518
Size: 648 3438 359 159 2042
Fragments arranged by size:
3438 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3515 3665 3700 4062 4739 5887 6132 1606
Size: 1909 150 35 362 677 1148 245 2120
Fragments arranged by size:
2120 1909 1148 677 362 245 150 35
NarI GG'CG_CC
Cuts at: 2644 2644
Size: 6646
NcoI C'CATG_G
Cuts at: 513 1065 2049 2637 2712 513
Size: 552 984 588 75 4447
Fragments arranged by size:
4447 984 588 552 75
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6646
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6646
NotI GC'GGCC_GC
Cuts at: 4093 4093
Size: 6646
NspI r_CATG'y
Cuts at: 1132 1229 2493 3391 4004 5549 1132
Size: 97 1264 898 613 1545 2229
Fragments arranged by size:
2229 1545 1264 898 613 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6646
PciI A'CATG_T
Cuts at: 5545 5545
Size: 6646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6646
PfoI T'CCnGG_A
Cuts at: 3004 3004
Size: 6646
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 4715 4747 6228 6260 3569
Size: 32 1114 32 1481 32 3955
Fragments arranged by size:
3955 1481 1114 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2071
Size: 6646
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6646
PsiI TTA'TAA
Cuts at: 3215 4216 3215
Size: 1001 5645
PstI C_TGCA'G
Cuts at: 830 2931 3616 3664 3998 830
Size: 2101 685 48 334 3478
Fragments arranged by size:
3478 2101 685 334 48
PvuI CG_AT'CG
Cuts at: 2001 3561 2001
Size: 1560 5086
PvuII CAG'CTG
Cuts at: 3515 3665 3515
Size: 150 6496
SacI G_AGCT'C
Cuts at: 3678 3678
Size: 6646
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SbfI CC_TGCA'GG
Cuts at: 3998 3998
Size: 6646
ScaI AGT'ACT
Cuts at: 3104 4894 6606 3104
Size: 1790 1712 3144
Fragments arranged by size:
3144 1790 1712
SfcI C'TryA_G
Cuts at: 826 1045 2089 2629 2927 3612 3660 3858 3994
Size: 219 1044 540 298 685 48 198 136
Cuts at: 3994 5129 5810 6001 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1044 685 681 540 298 219
198 191 136 48
SfoI GGC'GCC
Cuts at: 2645 2645
Size: 6646
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 3973 1872
Size: 2101 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3332 4750 5651 5913 820
Size: 197 926 1389 1418 901 262 1553
Fragments arranged by size:
1553 1418 1389 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4437 493
Size: 3944 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6646
SphI G_CATG'C
Cuts at: 1132 4004 1132
Size: 2872 3774
SspI AAT'ATT
Cuts at: 5 52 4570 5
Size: 47 4518 2081
Fragments arranged by size:
4518 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6646
StyI C'CwwG_G
Cuts at: 513 1065 2049 2188 2637 2712 3392 3760 4085
Size: 552 984 139 449 75 680 368 325
Cuts at: 4085 513
Size: 3074
Fragments arranged by size:
3074 984 680 552 449 368 325 139
75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3702 4673 4690 4843 5028 5473 325
Size: 1255 2122 971 17 153 185 445 1498
Fragments arranged by size:
2122 1498 1255 971 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3102 3147 3503
Size: 274 80 33 51 1443 1125 45 356
Cuts at: 3503 4892 6604 96
Size: 1389 1712 138
Fragments arranged by size:
1712 1443 1389 1125 356 274 138 80
51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6606 6638 6606
Size: 32 6614
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6646
XbaI T'CTAG_A
Cuts at: 3982 3982
Size: 6646
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6646
XmaI C'CCGG_G
Cuts at: 1870 3971 1870
Size: 2101 4545
XmnI GAAnn'nnTTC
Cuts at: 4775 4775
Size: 6646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4454 276
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I DraIII FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI