Restriction map for
pFN21AA0968
(Circular) (Six-base) MAPSORT of: pFN21AA0968.seq Check: 8034 from: 1 to: 6139
pFN21AA0968 6139 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2027 2027
Size: 6139
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3482 1937
Size: 1545 4594
Acc65I G'GTAC_C
Cuts at: 2631 3460 2631
Size: 829 5310
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 2432 2894 2921 4202 5586 5854 1632
Size: 593 207 462 27 1281 1384 268 1917
Fragments arranged by size:
1917 1384 1281 593 462 268 207 27
AflII C'TTAA_G
Cuts at: 820 1017 2801 820
Size: 197 1784 4158
Fragments arranged by size:
4158 1784 197
AflIII A'CryG_T
Cuts at: 1203 5038 6015 1203
Size: 3835 977 1327
Fragments arranged by size:
3835 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 3035 1166
Size: 1869 4270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3248 1184
Size: 2064 4075
AlwNI CAG_nnn'CTG
Cuts at: 2173 2346 2362 3316 3882 5454 2173
Size: 173 16 954 566 1572 2858
Fragments arranged by size:
2858 1572 954 566 173 16
ApaLI G'TGCA_C
Cuts at: 2399 3233 4196 5352 2399
Size: 834 963 1156 3186
Fragments arranged by size:
3186 1156 963 834
ApoI r'AATT_y
Cuts at: 1493 3448 3674 3826 5935 1493
Size: 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 2313 3464 1870
Size: 73 370 1151 4545
Fragments arranged by size:
4545 1151 370 73
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2450 2631 2687 3369 3418
Size: 325 217 627 663 181 56 682 49
Cuts at: 3418 3460 4915 618
Size: 42 1455 1842
Fragments arranged by size:
1842 1455 682 663 627 325 217 181
56 49 42
BanII G_rGCy'C
Cuts at: 1391 3458 1391
Size: 2067 4072
BbeI G_GCGC'C
Cuts at: 2691 3422 2691
Size: 731 5408
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3033 928
Size: 971 1134 4034
Fragments arranged by size:
4034 1134 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4330 4364 1903
Size: 34 2393 34 3678
Fragments arranged by size:
3678 2393 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2360 4031 5241 2360
Size: 1671 1210 3258
Fragments arranged by size:
3258 1671 1210
BclI T'GATC_A
Cuts at: 1548 2745 1548
Size: 1197 4942
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3381 4750 136
Size: 107 122 71 631 605 1709 1369 1525
Fragments arranged by size:
1709 1525 1369 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3410 6134 3410
Size: 2724 3415
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 2403 3237 4200 5356 1790
Size: 613 834 963 1156 2573
Fragments arranged by size:
2573 1156 963 834 613
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3340 3870 4828
Size: 416 53 83 399 24 1923 530 958
Cuts at: 4828 442
Size: 1753
Fragments arranged by size:
1923 1753 958 530 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2930
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2384 4799 1239
Size: 956 189 2415 2579
Fragments arranged by size:
2579 2415 956 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2429 4264 5129 5427 2429
Size: 1835 865 298 3141
Fragments arranged by size:
3141 1835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2154 2852 3350 4802 882
Size: 617 655 698 498 1452 2219
Fragments arranged by size:
2219 1452 698 655 617 498
BsaAI yAC'GTr
Cuts at: 493 1204 3930 493
Size: 711 2726 2702
Fragments arranged by size:
2726 2702 711
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2688 3419 3946 4328
Size: 53 83 186 936 1155 731 527 382
Cuts at: 4328 4963 275
Size: 635 1451
Fragments arranged by size:
1451 1155 936 731 635 527 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2945 4572 4970 5244 5391 5802 2945
Size: 1627 398 274 147 411 3282
Fragments arranged by size:
3282 1627 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5914
Fragments arranged by size:
5914 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 3368 1186
Size: 1075 1107 3957
Fragments arranged by size:
3957 1107 1075
BseYI C'CCAG_C
Cuts at: 5342 5838 5342
Size: 496 5643
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2005 2360 1163
Size: 573 269 355 4942
Fragments arranged by size:
4942 573 355 269
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3589 4350 5378 1540
Size: 275 186 1588 761 1028 2301
Fragments arranged by size:
2301 1588 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 3005 3237 4200 4285 5356 2403
Size: 602 232 963 85 1071 3186
Fragments arranged by size:
3186 1071 963 602 232 85
BsiWI C'GTAC_G
Cuts at: 2571 2571
Size: 6139
BsmI GAATG_Cn'
Cuts at: 3650 3743 3650
Size: 93 6046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2403 3005 3237 3458 4200 4285 5356
Size: 399 613 602 232 221 742 85 1071
Cuts at: 5356 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 613 602 399 232 221
85
BspEI T'CCGG_A
Cuts at: 2945 5802 2945
Size: 2857 3282
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2027 3494 844
Size: 1183 1467 3489
Fragments arranged by size:
3489 1467 1183
BsrBI CCG'CTC
Cuts at: 1394 1823 2826 4026 1394
Size: 429 1003 1200 3507
Fragments arranged by size:
3507 1200 1003 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3097 4634 4808 65
Size: 1183 1849 1537 174 1396
Fragments arranged by size:
1849 1537 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2684 4783 4970 1700
Size: 110 874 2099 187 2869
Fragments arranged by size:
2869 2099 874 187 110
BsrGI T'GTAC_A
Cuts at: 96 2613 96
Size: 2517 3622
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6139
BstBI TT'CG_AA
Cuts at: 3144 4943 3144
Size: 1799 4340
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstXI CCAn_nnnn'nTGG
Cuts at: 2491 2926 3413 2491
Size: 435 487 5217
Fragments arranged by size:
5217 487 435
BstYI r'GATC_y
Cuts at: 1972 2358 2740 2814 3410 3469 3836 4221 4238
Size: 386 382 74 596 59 367 385 17
Cuts at: 4238 5690 5776 5788 6134 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 596 386 385 382 367 346
86 74 59 17 12
Bsu36I CC'TnA_GG
Cuts at: 3150 3150
Size: 6139
BtgI C'CryG_G
Cuts at: 513 1065 2004 2832 2862 513
Size: 552 939 828 30 3790
Fragments arranged by size:
3790 939 828 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2688 2991 3274 487
Size: 683 56 1462 303 283 3352
Fragments arranged by size:
3352 1462 683 303 283 56
BtsI GCAGTG_nn'
Cuts at: 2377 2463 3655 4449 4469 2377
Size: 86 1192 794 20 4047
Fragments arranged by size:
4047 1192 794 86 20
ClaI AT'CG_AT
Cuts at: 1554 3190 1554
Size: 1636 4503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2110 2481
Size: 54 1425 50 275 18 177 103 371
Cuts at: 2481 2928 3586 4475 6104 8
Size: 447 658 889 1629 43
Fragments arranged by size:
1629 1425 889 658 447 371 275 177
103 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3586 1537
Size: 275 1774 4090
Fragments arranged by size:
4090 1774 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2054 2948 2966 4069 1353
Size: 678 23 894 18 1103 3423
Fragments arranged by size:
3423 1103 894 678 23 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2348 4726 5100 5246 387
Size: 1064 138 759 2378 374 146 1280
Fragments arranged by size:
2378 1280 1064 759 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2225 2384 2432 2894 2921 4202
Size: 393 563 30 159 48 462 27 1281
Cuts at: 4202 4799 5586 5854 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1281 787 597 563 462 393 268
159 48 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3170 3170
Size: 6139
EcoO109I rG'GnC_Cy
Cuts at: 2407 2566 3154 3175 2407
Size: 159 588 21 5371
Fragments arranged by size:
5371 588 159 21
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
EcoRV GAT'ATC
Cuts at: 2305 2540 2305
Size: 235 5904
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 6139
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 2691 3422 5286 1678
Size: 1013 731 1864 2531
Fragments arranged by size:
2531 1864 1013 731
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2687 2719 3089
Size: 32 135 32 653 32 430 32 370
Cuts at: 3089 3121 4780 4812 4854 4886 1373
Size: 32 1659 32 42 32 2626
Fragments arranged by size:
2626 1659 653 430 370 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3483 3729 669
Size: 988 281 1545 246 3079
Fragments arranged by size:
3079 1545 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KasI G'GCGC_C
Cuts at: 2687 3418 2687
Size: 731 5408
KpnI G_GTAC'C
Cuts at: 2635 3464 2635
Size: 829 5310
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2248 3330 5253 5437 1305
Size: 943 1082 1923 184 2007
Fragments arranged by size:
2007 1923 1082 943 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2112 2483 2930 10
Size: 54 1425 343 177 103 371 447 3219
Fragments arranged by size:
3219 1425 447 371 343 177 103 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 2921 3035 3116 4097 4456 4615
Size: 648 1323 432 114 81 981 359 159
Cuts at: 4615 518
Size: 2042
Fragments arranged by size:
2042 1323 981 648 432 359 159 114
81
MspA1I CmG'CkG
Cuts at: 1606 2789 3073 3555 4232 5380 5625 1606
Size: 1183 284 482 677 1148 245 2120
Fragments arranged by size:
2120 1183 1148 677 482 284 245
NaeI GCC'GGC
Cuts at: 2686 2686
Size: 6139
NarI GG'CG_CC
Cuts at: 2688 3419 2688
Size: 731 5408
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4648
Fragments arranged by size:
4648 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NgoMIV G'CCGG_C
Cuts at: 2684 2684
Size: 6139
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 2848 3497 5042 1132
Size: 97 1619 649 1545 2229
Fragments arranged by size:
2229 1619 1545 649 97
PasI CC'CwG_GG
Cuts at: 1348 3157 1348
Size: 1809 4330
PciI A'CATG_T
Cuts at: 5038 5038
Size: 6139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2389 3268 1108
Size: 1281 879 3979
Fragments arranged by size:
3979 1281 879
PfoI T'CCnGG_A
Cuts at: 2361 2533 3215 2361
Size: 172 682 5285
Fragments arranged by size:
5285 682 172
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2407 2566 2407
Size: 159 5980
PshAI GACnn'nnGTC
Cuts at: 1613 3362 1613
Size: 1749 4390
PsiI TTA'TAA
Cuts at: 3056 3709 3056
Size: 653 5486
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2624 2656 2624
Size: 32 6107
PstI C_TGCA'G
Cuts at: 830 3491 830
Size: 2661 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
PvuII CAG'CTG
Cuts at: 2789 3073 2789
Size: 284 5855
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2966 2054
Size: 912 5227
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SexAI A'CCwGG_T
Cuts at: 2274 3165 2274
Size: 891 5248
SfcI C'TryA_G
Cuts at: 826 1045 3487 4622 5303 5494 826
Size: 219 2442 1135 681 191 1471
Fragments arranged by size:
2442 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2689 3420 2689
Size: 731 5408
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 2315 3466 1872
Size: 443 1151 4545
Fragments arranged by size:
4545 1151 443
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 2801 4243 5144 5406 820
Size: 197 926 501 357 1442 901 262 1553
Fragments arranged by size:
1553 1442 926 901 501 357 262 197
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 2848 3497 1132
Size: 1716 649 3774
Fragments arranged by size:
3774 1716 649
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6139
StyI C'CwwG_G
Cuts at: 513 1065 2004 2736 3578 513
Size: 552 939 732 842 3074
Fragments arranged by size:
3074 939 842 732 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4166 4183 4336 4521 4966 325
Size: 1255 2586 17 153 185 445 1498
Fragments arranged by size:
2586 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2613 4385 6097
Size: 274 80 33 51 1443 636 1772 1712
Cuts at: 6097 96
Size: 138
Fragments arranged by size:
1772 1712 1443 636 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6099 6131 6099
Size: 32 6107
Tth111I GACn'n_nGTC
Cuts at: 1316 2276 2720 1316
Size: 960 444 4735
Fragments arranged by size:
4735 960 444
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2709 2709
Size: 6139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 2313 3464 1870
Size: 443 1151 4545
Fragments arranged by size:
4545 1151 443
XmnI GAAnn'nnTTC
Cuts at: 2879 4268 2879
Size: 1389 4750
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsmBI BstZ17I DraIII EcoICRI FseI FspAI NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SfiI SrfI SwaI