Restriction map for
pFN21AA0944
(Circular) (Six-base) MAPSORT of: pFN21AA0944.seq Check: 8475 from: 1 to: 16777
pFN21AA0944 16777 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6212 10004 13208 6212
Size: 3792 3204 9781
Fragments arranged by size:
9781 3792 3204
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3300 14587 278
Size: 53 83 186 936 1764 11287 2468
Fragments arranged by size:
11287 2468 1764 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 8375 13454 14120 1937
Size: 6438 5079 666 4594
Fragments arranged by size:
6438 5079 4594 666
Acc65I G'GTAC_C
Cuts at: 4155 6367 10859 13320 14098 4155
Size: 2212 4492 2461 778 6834
Fragments arranged by size:
6834 4492 2461 2212 778
AclI AA'CG_TT
Cuts at: 1563 2269 14904 15277 1563
Size: 706 12635 373 3063
Fragments arranged by size:
12635 3063 706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4079 4124 4248 4907 6356 6569 7029 9014
Size: 2447 45 124 659 1449 213 460 1985
Cuts at: 9014 9540 10485 11753 12132 12449 13412 14840 16224
Size: 526 945 1268 379 317 963 1428 1384
Cuts at: 16224 16492 1632
Size: 268 1917
Fragments arranged by size:
2447 1985 1917 1449 1428 1384 1268 963
945 659 526 460 379 317 268 213
124 45
AflII C'TTAA_G
Cuts at: 820 1017 9154 10517 820
Size: 197 8137 1363 7080
Fragments arranged by size:
8137 7080 1363 197
AflIII A'CryG_T
Cuts at: 1203 2563 5089 5639 6272 7269 8724 9381 9600
Size: 1360 2526 550 633 997 1455 657 219
Cuts at: 9600 11189 12861 15676 16653 1203
Size: 1589 1672 2815 977 1327
Fragments arranged by size:
2815 2526 1672 1589 1455 1360 1327 997
977 657 633 550 219
AgeI A'CCGG_T
Cuts at: 13661 15608 13661
Size: 1947 14830
AhdI GACnn_n'nnGTC
Cuts at: 5002 15506 5002
Size: 10504 6273
AleI CACnn'nnGTG
Cuts at: 1166 5276 5672 8609 1166
Size: 4110 396 2937 9334
Fragments arranged by size:
9334 4110 2937 396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2427 4239 6774 9425 1184
Size: 1243 1812 2535 2651 8536
Fragments arranged by size:
8536 2651 2535 1812 1243
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5535 5567 5535
Size: 32 16745
AlwNI CAG_nnn'CTG
Cuts at: 3182 4563 6227 7943 8685 11347 12279 13223 13868
Size: 1381 1664 1716 742 2662 932 944 645
Cuts at: 13868 14520 16092 3182
Size: 652 1572 3867
Fragments arranged by size:
3867 2662 1716 1664 1572 1381 944 932
742 652 645
ApaI G_GGCC'C
Cuts at: 10217 12411 10217
Size: 2194 14583
ApaLI G'TGCA_C
Cuts at: 12285 14834 15990 12285
Size: 2549 1156 13072
Fragments arranged by size:
13072 2549 1156
ApoI r'AATT_y
Cuts at: 1493 2754 2900 3579 3770 3999 4118 4328 4751
Size: 1261 146 679 191 229 119 210 423
Cuts at: 4751 4809 5324 6098 6579 6620 6745 7343 7388
Size: 58 515 774 481 41 125 598 45
Cuts at: 7388 7607 7966 8423 8435 8709 8895 8979 11734
Size: 219 359 457 12 274 186 84 2755
Cuts at: 11734 11751 12200 12341 12583 12725 12785 12986 13012
Size: 17 449 141 242 142 60 201 26
Cuts at: 13012 13497 13580 13848 13987 14086 14312 14464 16573
Size: 485 83 268 139 99 226 152 2109
Cuts at: 16573 1493
Size: 1697
Fragments arranged by size:
2755 2109 1697 1261 774 679 598 515
485 481 457 449 423 359 274 268
242 229 226 219 210 201 191 186
152 146 142 141 139 125 119 99
84 83 60 58 45 41 26 17
12
AseI AT'TA_AT
Cuts at: 160 1032 3671 6216 7886 9701 11895 15331 160
Size: 872 2639 2545 1670 1815 2194 3436 1606
Fragments arranged by size:
3436 2639 2545 2194 1815 1670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 16777
AvaI C'yCGr_G
Cuts at: 1870 1943 2694 8154 14102 1870
Size: 73 751 5460 5948 4545
Fragments arranged by size:
5948 5460 4545 751 73
AvrII C'CTAG_G
Cuts at: 5900 12796 5900
Size: 6896 9881
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 9475 9508 11088 11121 9475
Size: 33 1580 33 15131
Fragments arranged by size:
15131 1580 33 33
BamHI G'GATC_C
Cuts at: 8988 14107 14474 8988
Size: 5119 367 11291
Fragments arranged by size:
11291 5119 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 3460 4155 6367 10016
Size: 325 217 627 326 1347 695 2212 3649
Cuts at: 10016 10859 13320 13664 14098 15553 618
Size: 843 2461 344 434 1455 1842
Fragments arranged by size:
3649 2461 2212 1842 1455 1347 843 695
627 434 344 326 325 217
BanII G_rGCy'C
Cuts at: 1391 4980 5160 5956 8173 10217 11679 12411 14096
Size: 3589 180 796 2217 2044 1462 732 1685
Cuts at: 14096 1391
Size: 4072
Fragments arranged by size:
4072 3589 2217 2044 1685 1462 796 732
180
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2590 2723 6011 9181 11454 13400 13612
Size: 971 691 133 3288 3170 2273 1946 212
Cuts at: 13612 13743 928
Size: 131 3962
Fragments arranged by size:
3962 3288 3170 2273 1946 971 691 212
133 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 14968 15002 1903
Size: 34 13031 34 3678
Fragments arranged by size:
13031 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5572 8836 11204 14669 15879 5572
Size: 3264 2368 3465 1210 6470
Fragments arranged by size:
6470 3465 3264 2368 1210
BclI T'GATC_A
Cuts at: 1548 5853 9391 13066 1548
Size: 4305 3538 3675 5259
Fragments arranged by size:
5259 4305 3675 3538
BfrBI ATG'CAT
Cuts at: 2921 4463 9446 10140 2921
Size: 1542 4983 694 9558
Fragments arranged by size:
9558 4983 1542 694
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2164 9074 15388
Size: 107 122 71 631 605 492 6910 6314
Cuts at: 15388 136
Size: 1525
Fragments arranged by size:
6910 6314 1525 631 605 492 122 107
71
BglII A'GATC_T
Cuts at: 4135 6593 12832 13459 16772 4135
Size: 2458 6239 627 3313 4140
Fragments arranged by size:
6239 4140 3313 2458 627
BlpI GC'TnA_GC
Cuts at: 3519 4104 14194 3519
Size: 585 10090 6102
Fragments arranged by size:
10090 6102 585
Bme1580I G_kGCm'C
Cuts at: 1790 10217 12289 12411 13669 14838 15994 1790
Size: 8427 2072 122 1258 1169 1156 2573
Fragments arranged by size:
8427 2573 2072 1258 1169 1156 122
BmgBI CAC'GTC
Cuts at: 1318 12224 1318
Size: 10906 5871
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4573 4788 5533
Size: 416 53 83 399 24 3156 215 745
Cuts at: 5533 6009 7079 7953 10728 12961 14508 15466 442
Size: 476 1070 874 2775 2233 1547 958 1753
Fragments arranged by size:
3156 2775 2233 1753 1547 1070 958 874
745 476 416 399 215 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4692 9864 1056
Size: 3636 5172 7969
Fragments arranged by size:
7969 5172 3636
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5768 5768
Size: 16777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3927 4435 4454 4977 4994 5502 6894 7038
Size: 2688 508 19 523 17 508 1392 144
Cuts at: 7038 7300 8271 8499 9515 10272 10709 12333 13599
Size: 262 971 228 1016 757 437 1624 1266
Cuts at: 13599 15437 1239
Size: 1838 2579
Fragments arranged by size:
2688 2579 1838 1624 1392 1266 1016 971
757 523 508 508 437 262 228 144
19 17
Bpu10I CC'TnA_GC
Cuts at: 5813 5813
Size: 16777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2509 5264 5642 7559 9789 11762 12187 12524 13018
Size: 2755 378 1917 2230 1973 425 337 494
Cuts at: 13018 14902 15767 16065 2509
Size: 1884 865 298 3221
Fragments arranged by size:
3221 2755 2230 1973 1917 1884 865 494
425 378 337 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7276 15440 882
Size: 617 5777 8164 2219
Fragments arranged by size:
8164 5777 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2566 4962 6456 14568 493
Size: 711 1362 2396 1494 8112 2702
Fragments arranged by size:
8112 2702 2396 1494 1362 711
BsaBI GATnn'nnATC
Cuts at: 6609 7596 16771 6609
Size: 987 9175 6615
Fragments arranged by size:
9175 6615 987
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3297 14584 14966 15601
Size: 53 83 186 936 1764 11287 382 635
Cuts at: 15601 275
Size: 1451
Fragments arranged by size:
11287 1764 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7443 10739 12691 13661 15210 15608 15882 16029 16440
Size: 3296 1952 970 1549 398 274 147 411
Cuts at: 16440 7443
Size: 7780
Fragments arranged by size:
7780 3296 1952 1549 970 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4559 4589 5361 5391 5417
Size: 30 165 30 3156 30 772 30 26
Cuts at: 5417 5447 5706 5736 10860 10890 11176 11206 12840
Size: 30 259 30 5124 30 286 30 1634
Cuts at: 12840 12870 12924 12954 1178
Size: 30 54 30 5001
Fragments arranged by size:
5124 5001 3156 1634 772 286 259 165
54 30 30 30 30 30 30 30
30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3795 4615 5988 7803 8304 12533 13330 13602
Size: 2609 820 1373 1815 501 4229 797 272
Cuts at: 13602 13971 1186
Size: 369 3992
Fragments arranged by size:
4229 3992 2609 1815 1373 820 797 501
369 272
BseYI C'CCAG_C
Cuts at: 6308 6539 9257 10216 13111 13668 15980 16476 6308
Size: 231 2718 959 2895 557 2312 496 6609
Fragments arranged by size:
6609 2895 2718 2312 959 557 496 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3029 3084 10050 1163
Size: 573 1293 55 6966 7890
Fragments arranged by size:
7890 6966 1293 573 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2463 10374 14227 14988 16016 1540
Size: 275 186 462 7911 3853 761 1028 2301
Fragments arranged by size:
7911 3853 2301 1028 761 462 275 186
BsiHKAI G_wGCw'C
Cuts at: 3474 5160 5719 5980 6880 8970 12289 12306 14838
Size: 1686 559 261 900 2090 3319 17 2532
Cuts at: 14838 14923 15994 3474
Size: 85 1071 4257
Fragments arranged by size:
4257 3319 2532 2090 1686 1071 900 559
261 85 17
BsmI GAATG_Cn'
Cuts at: 2824 5124 6761 10957 11337 12227 12926 12975 13141
Size: 2300 1637 4196 380 890 699 49 166
Cuts at: 13141 14288 14381 2824
Size: 1147 93 5220
Fragments arranged by size:
5220 4196 2300 1637 1147 890 699 380
166 93 49
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3474 4980 5160 5719 5956 5980 6880
Size: 399 1684 1506 180 559 237 24 900
Cuts at: 6880 8173 8970 10217 11679 12289 12306 12411 13669
Size: 1293 797 1247 1462 610 17 105 1258
Cuts at: 13669 14096 14838 14923 15994 1391
Size: 427 742 85 1071 2174
Fragments arranged by size:
2174 1684 1506 1462 1293 1258 1247 1071
900 797 742 610 559 427 399 237
180 105 85 24 17
BspEI T'CCGG_A
Cuts at: 10739 12691 16440 10739
Size: 1952 3749 11076
Fragments arranged by size:
11076 3749 1952
BspHI T'CATG_A
Cuts at: 2952 3213 3300 4447 8632 8740 10261 11313 14666
Size: 261 87 1147 4185 108 1521 1052 3353
Cuts at: 14666 16396 2952
Size: 1730 3333
Fragments arranged by size:
4185 3353 3333 1730 1521 1147 1052 261
108 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6212 7324 10004 13208 14132 844
Size: 5368 1112 2680 3204 924 3489
Fragments arranged by size:
5368 3489 3204 2680 1112 924
BsrBI CCG'CTC
Cuts at: 1394 1823 11887 13951 14664 1394
Size: 429 10064 2064 713 3507
Fragments arranged by size:
10064 3507 2064 713 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2829 4476 6366 8499 9394 10407 12411
Size: 1183 1581 1647 1890 2133 895 1013 2004
Cuts at: 12411 13598 15272 15446 65
Size: 1187 1674 174 1396
Fragments arranged by size:
2133 2004 1890 1674 1647 1581 1396 1187
1183 1013 895 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 11817 12957 13661 15421 15608 1700
Size: 110 10007 1140 704 1760 187 2869
Fragments arranged by size:
10007 2869 1760 1140 704 187 110
BsrGI T'GTAC_A
Cuts at: 96 7586 10704 11963 96
Size: 7490 3118 1259 4910
Fragments arranged by size:
7490 4910 3118 1259
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 16777
BssSI C'ACGA_G
Cuts at: 14837 15849 14837
Size: 1012 15765
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 16777
BstBI TT'CG_AA
Cuts at: 8881 10885 15581 8881
Size: 2004 4696 10077
Fragments arranged by size:
10077 4696 2004
BstEII G'GTnAC_C
Cuts at: 1184 4589 1184
Size: 3405 13372
BstXI CCAn_nnnn'nTGG
Cuts at: 2769 2839 3983 6025 11536 11605 12362 13509 13623
Size: 70 1144 2042 5511 69 757 1147 114
Cuts at: 13623 2769
Size: 5923
Fragments arranged by size:
5923 5511 2042 1147 1144 757 114 70
69
BstYI r'GATC_y
Cuts at: 1972 2689 4135 4859 5266 6593 8988 10978 11134
Size: 717 1446 724 407 1327 2395 1990 156
Cuts at: 11134 12319 12832 13459 14107 14474 14859 14876 16328
Size: 1185 513 627 648 367 385 17 1452
Cuts at: 16328 16414 16426 16772 1972
Size: 86 12 346 1977
Fragments arranged by size:
2395 1990 1977 1452 1446 1327 1185 724
717 648 627 513 407 385 367 346
156 86 17 12
BstZ17I GTA'TAC
Cuts at: 8376 8376
Size: 16777
Bsu36I CC'TnA_GG
Cuts at: 5016 5982 11325 12720 13761 5016
Size: 966 5343 1395 1041 8032
Fragments arranged by size:
8032 5343 1395 1041 966
BtgI C'CryG_G
Cuts at: 513 1065 2512 6957 7787 10485 12324 513
Size: 552 1447 4445 830 2698 1839 4966
Fragments arranged by size:
4966 4445 2698 1839 1447 830 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3780 4730 5796 10014 487
Size: 683 56 2554 950 1066 4218 7250
Fragments arranged by size:
7250 4218 2554 1066 950 683 56
BtsI GCAGTG_nn'
Cuts at: 2017 3070 4752 6420 7101 14293 15087 15107 2017
Size: 1053 1682 1668 681 7192 794 20 3687
Fragments arranged by size:
7192 3687 1682 1668 1053 794 681 20
ClaI AT'CG_AT
Cuts at: 1554 7600 1554
Size: 6046 10731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4943 4978 7275 7310 13972 14007 529
Size: 35 4379 35 2297 35 6662 35 3299
Fragments arranged by size:
6662 4379 3299 2297 35 35 35 35
DraI TTT'AAA
Cuts at: 2685 2872 6939 7672 7977 9144 14081 2685
Size: 187 4067 733 305 1167 4937 5381
Fragments arranged by size:
5381 4937 4067 1167 733 305 187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2429 4619 7370 15784 809
Size: 507 1113 2190 2751 8414 1802
Fragments arranged by size:
8414 2751 2190 1802 1113 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 10044 10371
Size: 54 1425 50 275 18 198 8016 327
Cuts at: 10371 10539 13913 13975 14224 15113 16742 8
Size: 168 3374 62 249 889 1629 43
Fragments arranged by size:
8016 3374 1629 1425 889 327 275 249
198 168 62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 10371 14224 1537
Size: 275 8559 3853 4090
Fragments arranged by size:
8559 4090 3853 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4224 5351 7048 9213 10091 10816 11157 11568
Size: 2871 1127 1697 2165 878 725 341 411
Cuts at: 11568 14013 14707 1353
Size: 2445 694 3423
Fragments arranged by size:
3423 2871 2445 2165 1697 1127 878 725
694 411 341
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 15364 15738 15884 387
Size: 1064 138 13775 374 146 1280
Fragments arranged by size:
13775 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5158 5158
Size: 16777
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3927 4079 4124 4248 4435 4454 4907
Size: 393 2295 152 45 124 187 19 453
Cuts at: 4907 4977 4994 5502 6356 6569 6894 7029 7038
Size: 70 17 508 854 213 325 135 9
Cuts at: 7038 7300 8271 8499 9014 9515 9540 10272 10485
Size: 262 971 228 515 501 25 732 213
Cuts at: 10485 10709 11753 12132 12333 12449 13412 13599 14840
Size: 224 1044 379 201 116 963 187 1241
Cuts at: 14840 15437 16224 16492 1239
Size: 597 787 268 1524
Fragments arranged by size:
2295 1524 1241 1044 971 963 854 787
732 597 515 508 501 453 393 379
325 268 262 228 224 213 213 201
187 187 152 135 124 116 70 45
25 19 17 9
EcoNI CCTnn'n_nnAGG
Cuts at: 4163 5019 7731 13891 4163
Size: 856 2712 6160 7049
Fragments arranged by size:
7049 6160 2712 856
EcoO109I rG'GnC_Cy
Cuts at: 3124 7012 8282 8638 10213 12407 3124
Size: 3888 1270 356 1575 2194 7494
Fragments arranged by size:
7494 3888 2194 1575 1270 356
EcoRI G'AATT_C
Cuts at: 7343 12725 14086 14464 7343
Size: 5382 1361 378 9656
Fragments arranged by size:
9656 5382 1361 378
EcoRV GAT'ATC
Cuts at: 2893 3277 10726 10828 13150 2893
Size: 384 7449 102 2322 6520
Fragments arranged by size:
7449 6520 2322 384 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3514 6361 8521 10490 10574 11360 11416 11803 11863
Size: 2847 2160 1969 84 786 56 387 60
Cuts at: 11863 3514
Size: 8428
Fragments arranged by size:
8428 2847 2160 1969 786 387 84 60
56
FspI TGC'GCA
Cuts at: 12971 15283 12971
Size: 2312 14465
HaeII r_GCGC'y
Cuts at: 1678 15924 1678
Size: 14246 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2408 2440 4525 4557 4559
Size: 32 135 32 836 32 2085 32 2
Cuts at: 4559 4591 4988 5020 5237 5251 5269 5283 5602
Size: 32 397 32 217 14 18 14 319
Cuts at: 5602 5634 5738 5770 6464 6496 6793 6825 6974
Size: 32 104 32 694 32 297 32 149
Cuts at: 6974 7006 7501 7533 7682 7714 7891 7923 8605
Size: 32 495 32 149 32 177 32 682
Cuts at: 8605 8637 9256 9288 9887 9919 10832 10864 10963
Size: 32 619 32 599 32 913 32 99
Cuts at: 10963 10966 10995 10998 12654 12668 12673 12686 12700
Size: 3 29 3 1656 14 5 13 14
Cuts at: 12700 12705 15418 15450 15492 15524 1373
Size: 5 2713 32 42 32 2626
Fragments arranged by size:
2713 2626 2085 1656 913 836 694 682
619 599 495 397 319 297 217 177
149 149 135 104 99 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 29 18 14 14 14 14
13 5 5 3 3 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3934 9484 10603 12537 14066 14121
Size: 988 281 1996 5550 1119 1934 1529 55
Cuts at: 14121 14367 669
Size: 246 3079
Fragments arranged by size:
5550 3079 1996 1934 1529 1119 988 281
246 55
HindIII A'AGCT_T
Cuts at: 748 9610 10118 748
Size: 8862 508 7407
Fragments arranged by size:
8862 7407 508
HpaI GTT'AAC
Cuts at: 9484 14367 9484
Size: 4883 11894
KpnI G_GTAC'C
Cuts at: 4159 6371 10863 13324 14102 4159
Size: 2212 4492 2461 778 6834
Fragments arranged by size:
6834 4492 2461 2212 778
MfeI C'AATT_G
Cuts at: 3044 6730 11214 14376 3044
Size: 3686 4484 3162 5445
Fragments arranged by size:
5445 4484 3686 3162
MluI A'CGCG_T
Cuts at: 16653 16653
Size: 16777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2299 4170 4803 6057 6808 9010 9810 15891
Size: 994 1871 633 1254 751 2202 800 6081
Cuts at: 15891 16075 1305
Size: 184 2007
Fragments arranged by size:
6081 2202 2007 1871 1254 994 800 751
633 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10541 13977 10
Size: 54 1425 343 8709 3436 2810
Fragments arranged by size:
8709 3436 2810 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2193 2817 2951 3558 4529 5276 5672
Size: 648 1027 624 134 607 971 747 396
Cuts at: 5672 8609 10266 10400 11603 12416 14059 14735 15094
Size: 2937 1657 134 1203 813 1643 676 359
Cuts at: 15094 15253 518
Size: 159 2042
Fragments arranged by size:
2937 2042 1657 1643 1203 1027 971 813
747 676 648 624 607 396 359 159
134 134
MspA1I CmG'CkG
Cuts at: 1606 2074 2137 2429 3118 4153 5039 5993 6478
Size: 468 63 292 689 1035 886 954 485
Cuts at: 6478 9334 10370 10463 10702 12708 13177 14193 14870
Size: 2856 1036 93 239 2006 469 1016 677
Cuts at: 14870 16018 16263 1606
Size: 1148 245 2120
Fragments arranged by size:
2856 2120 2006 1148 1036 1035 1016 954
886 689 677 485 469 468 292 245
239 93 63
NcoI C'CATG_G
Cuts at: 513 1065 2512 6957 7787 10485 12324 513
Size: 552 1447 4445 830 2698 1839 4966
Fragments arranged by size:
4966 4445 2698 1839 1447 830 552
NdeI CA'TA_TG
Cuts at: 387 4459 10411 387
Size: 4072 5952 6753
Fragments arranged by size:
6753 5952 4072
NheI G'CTAG_C
Cuts at: 1052 4688 9860 1052
Size: 3636 5172 7969
Fragments arranged by size:
7969 5172 3636
NotI GC'GGCC_GC
Cuts at: 14224 14224
Size: 16777
NsiI A_TGCA'T
Cuts at: 2923 4465 9448 10142 2923
Size: 1542 4983 694 9558
Fragments arranged by size:
9558 4983 1542 694
NspI r_CATG'y
Cuts at: 1132 1229 5093 5643 6276 7273 8326 8728 9385
Size: 97 3864 550 633 997 1053 402 657
Cuts at: 9385 9416 9604 11193 12865 14135 15680 1132
Size: 31 188 1589 1672 1270 1545 2229
Fragments arranged by size:
3864 2229 1672 1589 1545 1270 1053 997
657 633 550 402 188 97 31
PasI CC'CwG_GG
Cuts at: 1348 6299 10864 1348
Size: 4951 4565 7261
Fragments arranged by size:
7261 4951 4565
PciI A'CATG_T
Cuts at: 5089 5639 6272 7269 8724 9381 9600 11189 12861
Size: 550 633 997 1455 657 219 1589 1672
Cuts at: 12861 15676 5089
Size: 2815 6190
Fragments arranged by size:
6190 2815 1672 1589 1455 997 657 633
550 219
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4563 8294 9501 12489 13369 1108
Size: 3455 3731 1207 2988 880 4516
Fragments arranged by size:
4516 3731 3455 2988 1207 880
PfoI T'CCnGG_A
Cuts at: 8984 12310 13805 8984
Size: 3326 1495 11956
Fragments arranged by size:
11956 3326 1495
PmeI GTTT'AAAC
Cuts at: 14081 14081
Size: 16777
PmlI CAC'GTG
Cuts at: 4962 4962
Size: 16777
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 14846 14878 16359 16391 14846
Size: 32 1481 32 15232
Fragments arranged by size:
15232 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3124 7012 8282 8638 3124
Size: 3888 1270 356 11263
Fragments arranged by size:
11263 3888 1270 356
PshAI GACnn'nnGTC
Cuts at: 1613 6433 1613
Size: 4820 11957
PsiI TTA'TAA
Cuts at: 6287 8342 8421 12206 14347 6287
Size: 2055 79 3785 2141 8717
Fragments arranged by size:
8717 3785 2141 2055 79
PspOMI G'GGCC_C
Cuts at: 10213 12407 10213
Size: 2194 14583
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7648 7680 11139 11171 7648
Size: 32 3459 32 13254
Fragments arranged by size:
13254 3459 32 32
PstI C_TGCA'G
Cuts at: 830 3123 4204 10254 10462 12606 14129 830
Size: 2293 1081 6050 208 2144 1523 3478
Fragments arranged by size:
6050 3478 2293 2144 1523 1081 208
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 16777
PvuII CAG'CTG
Cuts at: 2074 2137 2429 3118 5039 5993 6478 10463 10702
Size: 63 292 689 1921 954 485 3985 239
Cuts at: 10702 12708 13177 2074
Size: 2006 469 5674
Fragments arranged by size:
5674 3985 2006 1921 954 689 485 469
292 239 63
SacI G_AGCT'C
Cuts at: 5160 5160
Size: 16777
SalI G'TCGA_C
Cuts at: 1936 14119 1936
Size: 12183 4594
SapI GCTCTTCn'nnn_
Cuts at: 5351 5351
Size: 16777
SbfI CC_TGCA'GG
Cuts at: 14129 14129
Size: 16777
ScaI AGT'ACT
Cuts at: 2383 6171 12600 15025 16737 2383
Size: 3788 6429 2425 1712 2423
Fragments arranged by size:
6429 3788 2425 2423 1712
SexAI A'CCwGG_T
Cuts at: 10596 11699 10596
Size: 1103 15674
SfcI C'TryA_G
Cuts at: 826 1045 2472 3119 4200 4342 6036 8034 8851
Size: 219 1427 647 1081 142 1694 1998 817
Cuts at: 8851 9627 9906 10250 10365 10458 10777 12602 13455
Size: 776 279 344 115 93 319 1825 853
Cuts at: 13455 14125 15260 15941 16132 826
Size: 670 1135 681 191 1471
Fragments arranged by size:
1998 1825 1694 1471 1427 1135 1081 853
817 776 681 670 647 344 319 279
219 191 142 115 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 16777
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 16777
SmaI CCC'GGG
Cuts at: 1872 14104 1872
Size: 12232 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2488 2694 5243 5621 7538 9154
Size: 197 926 545 206 2549 378 1917 1616
Cuts at: 9154 9804 10517 11777 12166 12503 13033 14881 15782
Size: 650 713 1260 389 337 530 1848 901
Cuts at: 15782 16044 820
Size: 262 1553
Fragments arranged by size:
2549 1917 1848 1616 1553 1260 926 901
713 650 545 530 389 378 337 262
206 197
SnaBI TAC'GTA
Cuts at: 493 6456 14568 493
Size: 5963 8112 2702
Fragments arranged by size:
8112 5963 2702
SpeI A'CTAG_T
Cuts at: 152 6851 8251 152
Size: 6699 1400 8678
Fragments arranged by size:
8678 6699 1400
SphI G_CATG'C
Cuts at: 1132 14135 1132
Size: 13003 3774
SspI AAT'ATT
Cuts at: 5 52 3016 4700 5183 5776 7923 8056 8338
Size: 47 2964 1684 483 593 2147 133 282
Cuts at: 8338 10029 10282 10897 12808 13420 14701 5
Size: 1691 253 615 1911 612 1281 2081
Fragments arranged by size:
2964 2147 2081 1911 1691 1684 1281 615
612 593 483 282 253 133 47
StuI AGG'CCT
Cuts at: 1517 10650 11534 1517
Size: 9133 884 6760
Fragments arranged by size:
9133 6760 884
StyI C'CwwG_G
Cuts at: 513 1065 2512 2768 3025 5900 5968 6018 6957
Size: 552 1447 256 257 2875 68 50 939
Cuts at: 6957 7787 10485 10651 11529 11535 12258 12324 12403
Size: 830 2698 166 878 6 723 66 79
Cuts at: 12403 12796 14025 14216 513
Size: 393 1229 191 3074
Fragments arranged by size:
3074 2875 2698 1447 1229 939 878 830
723 552 393 257 256 191 166 79
68 66 50 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4677 7291 7919 12959 14804 14821 14974
Size: 1255 3097 2614 628 5040 1845 17 153
Cuts at: 14974 15159 15604 325
Size: 185 445 1498
Fragments arranged by size:
5040 3097 2614 1845 1498 1255 628 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2150 2381 2845
Size: 274 80 33 51 1443 173 231 464
Cuts at: 2845 3615 5587 6169 7586 10704 11186 11963 12598
Size: 770 1972 582 1417 3118 482 777 635
Cuts at: 12598 13354 15023 16735 96
Size: 756 1669 1712 138
Fragments arranged by size:
3118 1972 1712 1669 1443 1417 777 770
756 635 582 482 464 274 231 173
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5706 5738 7701 7733 10860 10892 11217 11249 11343
Size: 32 1963 32 3127 32 325 32 94
Cuts at: 11343 11375 12924 12956 13638 13670 16737 16769 5706
Size: 32 1549 32 682 32 3067 32 5714
Fragments arranged by size:
5714 3127 3067 1963 1549 682 325 94
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 11063 1316
Size: 9747 7030
XbaI T'CTAG_A
Cuts at: 8387 8440 14113 8387
Size: 53 5673 11051
Fragments arranged by size:
11051 5673 53
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5419 5477 7062 9820 5419
Size: 58 1585 2758 12376
Fragments arranged by size:
12376 2758 1585 58
XhoI C'TCGA_G
Cuts at: 1943 2694 1943
Size: 751 16026
XmaI C'CCGG_G
Cuts at: 1870 14102 1870
Size: 12232 4545
XmnI GAAnn'nnTTC
Cuts at: 2597 6658 8736 10360 11151 13562 14906 2597
Size: 4061 2078 1624 791 2411 1344 4468
Fragments arranged by size:
4468 4061 2411 2078 1624 1344 791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3298 14585 276
Size: 53 83 186 936 1764 11287 2468
Fragments arranged by size:
11287 2468 1764 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BbeI BbvCI BsiWI BsmBI DraIII FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI
RsrII SacII SanDI SfiI SfoI SrfI SwaI