Restriction map for
pFN21AA0943
(Circular) (Six-base) MAPSORT of: pFN21AA0943.seq Check: 8633 from: 1 to: 5884
pFN21AA0943 5884 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3096 3694 278
Size: 53 83 186 936 1560 598 2468
Fragments arranged by size:
2468 1560 936 598 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2314 2480 2753 3000 3947 5331 5599 1632
Size: 682 166 273 247 947 1384 268 1917
Fragments arranged by size:
1917 1384 947 682 273 268 247 166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 4783 5760 1203
Size: 3580 977 1327
Fragments arranged by size:
3580 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 2525 1166
Size: 1359 4525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5884
AlwNI CAG_nnn'CTG
Cuts at: 2458 2761 3627 5199 2458
Size: 303 866 1572 3143
Fragments arranged by size:
3143 1572 866 303
ApaI G_GGCC'C
Cuts at: 2439 2439
Size: 5884
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 3193 3419 3571 5680 1493
Size: 1700 226 152 2109 1697
Fragments arranged by size:
2109 1700 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 3209 1870
Size: 73 1266 4545
Fragments arranged by size:
4545 1266 73
AvrII C'CTAG_G
Cuts at: 2278 2278
Size: 5884
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2274 2576 3000 3035 3205
Size: 325 217 627 487 302 424 35 170
Cuts at: 3205 4660 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 487 424 325 302 217
170 35
BanII G_rGCy'C
Cuts at: 1391 2079 2439 2492 2700 2826 2868 3203 1391
Size: 688 360 53 208 126 42 335 4072
Fragments arranged by size:
4072 688 360 335 208 126 53 42
BbeI G_GCGC'C
Cuts at: 2580 2580
Size: 5884
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2467 3168 928
Size: 971 568 701 3644
Fragments arranged by size:
3644 971 701 568
BbvCI CC'TCA_GC
Cuts at: 2634 2634
Size: 5884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2914 2948 4075 4109 1903
Size: 34 977 34 1127 34 3678
Fragments arranged by size:
3678 1127 977 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2079 3776 4986 2079
Size: 1697 1210 2977
Fragments arranged by size:
2977 1697 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4495 136
Size: 107 122 71 631 605 2823 1525
Fragments arranged by size:
2823 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2127 5879 2127
Size: 3752 2132
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2439 3040 3945 5101 1790
Size: 649 601 905 1156 2573
Fragments arranged by size:
2573 1156 905 649 601
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5884
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 2749 3615
Size: 416 53 83 399 24 682 650 866
Cuts at: 3615 4573 442
Size: 958 1753
Fragments arranged by size:
1753 958 866 682 650 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4544 1239
Size: 3305 2579
Bpu10I CC'TnA_GC
Cuts at: 2634 2634
Size: 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4009 4874 5172 4009
Size: 865 298 4721
Fragments arranged by size:
4721 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2060 2656 4547 882
Size: 617 561 596 1891 2219
Fragments arranged by size:
2219 1891 617 596 561
BsaAI yAC'GTr
Cuts at: 493 1204 2484 2722 3675 493
Size: 711 1280 238 953 2702
Fragments arranged by size:
2702 1280 953 711 238
BsaBI GATnn'nnATC
Cuts at: 5878 5878
Size: 5884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2577 2766 3093 3691
Size: 53 83 186 936 1044 189 327 598
Cuts at: 3691 4073 4708 275
Size: 382 635 1451
Fragments arranged by size:
1451 1044 936 635 598 382 327 189
186 83 53
BsaWI w'CCGG_w
Cuts at: 2037 4317 4715 4989 5136 5547 2037
Size: 2280 398 274 147 411 2374
Fragments arranged by size:
2374 2280 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2622 2652 1178
Size: 30 165 30 1219 30 4410
Fragments arranged by size:
4410 1219 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2834 1186
Size: 1648 4236
BseYI C'CCAG_C
Cuts at: 2217 5087 5583 2217
Size: 2870 496 2518
Fragments arranged by size:
2870 2518 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2572 2592 1163
Size: 573 836 20 4455
Fragments arranged by size:
4455 836 573 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2605 3334 4095 5123 1540
Size: 275 186 604 729 761 1028 2301
Fragments arranged by size:
2301 1028 761 729 604 275 186
BsiHKAI G_wGCw'C
Cuts at: 2492 2826 3945 4030 5101 2492
Size: 334 1119 85 1071 3275
Fragments arranged by size:
3275 1119 1071 334 85
BsmI GAATG_Cn'
Cuts at: 2345 3395 3488 2345
Size: 1050 93 4741
Fragments arranged by size:
4741 1050 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2720 2720
Size: 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2439 2492 2700 2826 2868 3040
Size: 399 289 360 53 208 126 42 172
Cuts at: 3040 3203 3945 4030 5101 1391
Size: 163 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 360 289 208 172
163 126 85 53 42
BspEI T'CCGG_A
Cuts at: 5547 5547
Size: 5884
BspHI T'CATG_A
Cuts at: 2745 3773 5503 2745
Size: 1028 1730 3126
Fragments arranged by size:
3126 1730 1028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3239 844
Size: 2395 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2505 2517 4379 4553 65
Size: 1183 1257 12 1862 174 1396
Fragments arranged by size:
1862 1396 1257 1183 174 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2271 2604 4528 4715 1700
Size: 110 461 333 1924 187 2869
Fragments arranged by size:
2869 1924 461 333 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 3944 4956 3944
Size: 1012 4872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5884
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 2638 1184
Size: 1454 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 2959 2959
Size: 5884
BstYI r'GATC_y
Cuts at: 1972 2127 3214 3581 3966 3983 5435 5521 5533
Size: 155 1087 367 385 17 1452 86 12
Cuts at: 5533 5879 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1087 385 367 346 155 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2657 2859 513
Size: 552 939 653 202 3538
Fragments arranged by size:
3538 939 653 552 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2917 2938 487
Size: 683 56 1691 21 3433
Fragments arranged by size:
3433 1691 683 56 21
BtsI GCAGTG_nn'
Cuts at: 2580 2608 2737 2902 3400 4194 4214 2580
Size: 28 129 165 498 794 20 4250
Fragments arranged by size:
4250 794 498 165 129 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3331 4220 5849
Size: 54 1425 50 275 18 1501 889 1629
Cuts at: 5849 8
Size: 43
Fragments arranged by size:
1629 1501 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2303 3814 1353
Size: 950 1511 3423
Fragments arranged by size:
3423 1511 950
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4471 4845 4991 387
Size: 1064 138 2882 374 146 1280
Fragments arranged by size:
2882 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2490 2824 2490
Size: 334 5550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2314 2480 2753 3000 3947 4544 5331
Size: 393 682 166 273 247 947 597 787
Cuts at: 5331 5599 1239
Size: 268 1524
Fragments arranged by size:
1524 947 787 682 597 393 273 268
247 166
EcoO109I rG'GnC_Cy
Cuts at: 2198 2455 2198
Size: 257 5627
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 5884
FspI TGC'GCA
Cuts at: 4390 4390
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 2580 2793 5031 1678
Size: 902 213 2238 2531
Fragments arranged by size:
2531 2238 902 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2129 2149 2161 2232
Size: 32 135 32 545 12 20 12 71
Cuts at: 2232 2264 2622 2654 4525 4557 4599 4631 1373
Size: 32 358 32 1871 32 42 32 2626
Fragments arranged by size:
2626 1871 545 358 135 71 42 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3228 3474 669
Size: 988 281 1290 246 3079
Fragments arranged by size:
3079 1290 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2465 748
Size: 1717 4167
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KasI G'GCGC_C
Cuts at: 2576 2576
Size: 5884
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2625 4998 5182 1305
Size: 1320 2373 184 2007
Fragments arranged by size:
2373 2007 1320 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4062
Fragments arranged by size:
4062 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2510 2525 2957 3842 4201 4360 518
Size: 648 1344 15 432 885 359 159 2042
Fragments arranged by size:
2042 1344 885 648 432 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 3300 3977 5125 5370 1606
Size: 1694 677 1148 245 2120
Fragments arranged by size:
2120 1694 1148 677 245
NaeI GCC'GGC
Cuts at: 2273 2273
Size: 5884
NarI GG'CG_CC
Cuts at: 2577 2577
Size: 5884
NcoI C'CATG_G
Cuts at: 513 1065 2004 2657 513
Size: 552 939 653 3740
Fragments arranged by size:
3740 939 653 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NgoMIV G'CCGG_C
Cuts at: 2271 2271
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 2230 3242 4787 1132
Size: 97 1001 1012 1545 2229
Fragments arranged by size:
2229 1545 1012 1001 97
PasI CC'CwG_GG
Cuts at: 1348 2761 1348
Size: 1413 4471
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2186 2263 2761 1108
Size: 1078 77 498 4231
Fragments arranged by size:
4231 1078 498 77
PfoI T'CCnGG_A
Cuts at: 2886 2886
Size: 5884
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2455 2455
Size: 5884
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PspOMI G'GGCC_C
Cuts at: 2435 2435
Size: 5884
PstI C_TGCA'G
Cuts at: 830 2596 3236 830
Size: 1766 640 3478
Fragments arranged by size:
3478 1766 640
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
SacI G_AGCT'C
Cuts at: 2492 2826 2492
Size: 334 5550
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SfcI C'TryA_G
Cuts at: 826 1045 2592 3232 4367 5048 5239 826
Size: 219 1547 640 1135 681 191 1471
Fragments arranged by size:
1547 1471 1135 681 640 219 191
SfoI GGC'GCC
Cuts at: 2578 2578
Size: 5884
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 3211 1872
Size: 1339 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3988 4889 5151 820
Size: 197 926 2045 901 262 1553
Fragments arranged by size:
2045 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 2230 3242 1132
Size: 1098 1012 3774
Fragments arranged by size:
3774 1098 1012
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 2717 1517
Size: 1200 4684
StyI C'CwwG_G
Cuts at: 513 1065 2004 2278 2493 2657 3323 513
Size: 552 939 274 215 164 666 3074
Fragments arranged by size:
3074 939 666 552 274 215 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3911 3928 4081 4266 4711 325
Size: 1255 2331 17 153 185 445 1498
Fragments arranged by size:
2331 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4130 5842 96
Size: 274 80 33 51 1443 2153 1712 138
Fragments arranged by size:
2153 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2572 2604 5844 5876 2572
Size: 32 3240 32 2580
Fragments arranged by size:
3240 2580 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 2147 3220 2147
Size: 1073 4811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3052 3052
Size: 5884
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 3209 1870
Size: 1339 4545
XmnI GAAnn'nnTTC
Cuts at: 2058 4013 2058
Size: 1955 3929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3094 3692 276
Size: 53 83 186 936 1560 598 2468
Fragments arranged by size:
2468 1560 936 598 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI