Restriction map for
pFN21AA0937
(Circular) (Six-base) MAPSORT of: pFN21AA0937.seq Check: 408 from: 1 to: 6562
pFN21AA0937 6562 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2761 2761
Size: 6562
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4372 278
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3333 3905 1937
Size: 1396 572 4594
Fragments arranged by size:
4594 1396 572
Acc65I G'GTAC_C
Cuts at: 3883 3883
Size: 6562
AclI AA'CG_TT
Cuts at: 1563 4689 5062 1563
Size: 3126 373 3063
Fragments arranged by size:
3126 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2774 3656 4625 6009 6277 1632
Size: 1142 882 969 1384 268 1917
Fragments arranged by size:
1917 1384 1142 969 882 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6365
AflIII A'CryG_T
Cuts at: 1203 5461 6438 1203
Size: 4258 977 1327
Fragments arranged by size:
4258 1327 977
AgeI A'CCGG_T
Cuts at: 5393 5393
Size: 6562
AhdI GACnn_n'nnGTC
Cuts at: 2185 5291 2185
Size: 3106 3456
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6562
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2153 2681 1184
Size: 969 528 5065
Fragments arranged by size:
5065 969 528
AlwNI CAG_nnn'CTG
Cuts at: 2708 2902 4305 5877 2708
Size: 194 1403 1572 3393
Fragments arranged by size:
3393 1572 1403 194
ApaI G_GGCC'C
Cuts at: 2637 3278 2637
Size: 641 5921
ApaLI G'TGCA_C
Cuts at: 4619 5775 4619
Size: 1156 5406
ApoI r'AATT_y
Cuts at: 1493 3871 4097 4249 6358 1493
Size: 2378 226 152 2109 1697
Fragments arranged by size:
2378 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5116 160
Size: 872 4084 1606
Fragments arranged by size:
4084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
AvaI C'yCGr_G
Cuts at: 1870 1943 2919 3579 3887 1870
Size: 73 976 660 308 4545
Fragments arranged by size:
4545 976 660 308 73
BamHI G'GATC_C
Cuts at: 3012 3892 4259 3012
Size: 880 367 5315
Fragments arranged by size:
5315 880 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2469 2735 3210 3431 3683
Size: 325 217 627 682 266 475 221 252
Cuts at: 3683 3883 5338 618
Size: 200 1455 1842
Fragments arranged by size:
1842 1455 682 627 475 325 266 252
221 217 200
BanII G_rGCy'C
Cuts at: 1391 2637 2822 3278 3881 1391
Size: 1246 185 456 603 4072
Fragments arranged by size:
4072 1246 603 456 185
BbeI G_GCGC'C
Cuts at: 2473 2473
Size: 6562
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2762 3431 928
Size: 971 863 669 4059
Fragments arranged by size:
4059 971 863 669
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4753 4787 1903
Size: 34 50 34 2732 34 3678
Fragments arranged by size:
3678 2732 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4454 5664 4454
Size: 1210 5352
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6562
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5173 136
Size: 107 122 71 631 605 3501 1525
Fragments arranged by size:
3501 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6557 6557
Size: 6562
BlpI GC'TnA_GC
Cuts at: 3979 3979
Size: 6562
Bme1580I G_kGCm'C
Cuts at: 1790 2637 3278 4623 5779 1790
Size: 847 641 1345 1156 2573
Fragments arranged by size:
2573 1345 1156 847 641
BmgBI CAC'GTC
Cuts at: 1318 3331 1318
Size: 2013 4549
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2222 2575 2640
Size: 416 53 83 399 24 805 353 65
Cuts at: 2640 2814 2908 2948 3018 4293 5251 442
Size: 174 94 40 70 1275 958 1753
Fragments arranged by size:
1753 1275 958 805 416 399 353 174
94 83 70 65 53 40 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3769 3769
Size: 6562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2697 2776 3426 3673 5222 1239
Size: 1458 79 650 247 1549 2579
Fragments arranged by size:
2579 1549 1458 650 247 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4687 5552 5850 4687
Size: 865 298 5399
Fragments arranged by size:
5399 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5225 882
Size: 617 3726 2219
Fragments arranged by size:
3726 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2404 4353 493
Size: 711 1200 1949 2702
Fragments arranged by size:
2702 1949 1200 711
BsaBI GATnn'nnATC
Cuts at: 6556 6556
Size: 6562
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2062 2312 2470 4369
Size: 53 83 186 936 529 250 158 1899
Cuts at: 4369 4751 5386 275
Size: 382 635 1451
Fragments arranged by size:
1899 1451 936 635 529 382 250 186
158 83 53
BsaWI w'CCGG_w
Cuts at: 2778 2855 3507 3544 3607 4995 5393 5667 5814
Size: 77 652 37 63 1388 398 274 147
Cuts at: 5814 6225 2778
Size: 411 3115
Fragments arranged by size:
3115 1388 652 411 398 274 147 77
63 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2288 2318 3424
Size: 30 165 30 834 30 21 30 1106
Cuts at: 3424 3454 1178
Size: 30 4286
Fragments arranged by size:
4286 1106 834 165 30 30 30 30
30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2245 1186
Size: 1059 5503
BseYI C'CCAG_C
Cuts at: 2074 2140 2445 5765 6261 2074
Size: 66 305 3320 496 2375
Fragments arranged by size:
3320 2375 496 305 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3045 3214 1163
Size: 573 1309 169 4511
Fragments arranged by size:
4511 1309 573 169
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 2056 4012 4773 5801 1540
Size: 275 186 21 34 1956 761 1028 2301
Fragments arranged by size:
2301 1956 1028 761 275 186 34 21
BsiHKAI G_wGCw'C
Cuts at: 2052 4623 4708 5779 2052
Size: 2571 85 1071 2835
Fragments arranged by size:
2835 2571 1071 85
BsmI GAATG_Cn'
Cuts at: 3534 4073 4166 3534
Size: 539 93 5930
Fragments arranged by size:
5930 539 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2165 2165
Size: 6562
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2637 2822 3278 3881 4623 4708
Size: 399 262 585 185 456 603 742 85
Cuts at: 4708 5779 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 603 585 456 399 262
185 85
BspEI T'CCGG_A
Cuts at: 2778 3507 3607 6225 2778
Size: 729 100 2618 3115
Fragments arranged by size:
3115 2618 729 100
BspHI T'CATG_A
Cuts at: 3018 4451 6181 3018
Size: 1433 1730 3399
Fragments arranged by size:
3399 1730 1433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2190 2761 2901 3410 3917 844
Size: 1346 571 140 509 507 3489
Fragments arranged by size:
3489 1346 571 509 507 140
BsrBI CCG'CTC
Cuts at: 1394 1823 4449 1394
Size: 429 2626 3507
Fragments arranged by size:
3507 2626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2907 5057 5231 65
Size: 1183 1659 2150 174 1396
Fragments arranged by size:
2150 1659 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 2466 5206 5393 1700
Size: 110 301 355 2740 187 2869
Fragments arranged by size:
2869 2740 355 301 187 110
BsrGI T'GTAC_A
Cuts at: 96 3360 96
Size: 3264 3298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6562
BssSI C'ACGA_G
Cuts at: 3647 4622 5634 3647
Size: 975 1012 4575
Fragments arranged by size:
4575 1012 975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6562
BstBI TT'CG_AA
Cuts at: 5366 5366
Size: 6562
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6562
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2722 3241 3793 2011
Size: 711 519 552 4780
Fragments arranged by size:
4780 711 552 519
BstYI r'GATC_y
Cuts at: 1972 3012 3892 4259 4644 4661 6113 6199 6211
Size: 1040 880 367 385 17 1452 86 12
Cuts at: 6211 6557 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1040 880 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2511 3262 2511
Size: 751 5811
BtgI C'CryG_G
Cuts at: 513 1065 2022 2421 2490 2613 2814 3769 513
Size: 552 957 399 69 123 201 955 3306
Fragments arranged by size:
3306 957 955 552 399 201 123 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2751 487
Size: 683 56 1525 4298
Fragments arranged by size:
4298 1525 683 56
BtsI GCAGTG_nn'
Cuts at: 2696 3398 3843 4078 4872 4892 2696
Size: 702 445 235 794 20 4366
Fragments arranged by size:
4366 794 702 445 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6527
DraI TTT'AAA
Cuts at: 3866 3866
Size: 6562
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5569 809
Size: 507 4253 1802
Fragments arranged by size:
4253 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2053 2525
Size: 54 1425 50 275 18 189 34 472
Cuts at: 2525 2871 2904 3325 3484 3766 4009 4898 6527
Size: 346 33 421 159 282 243 889 1629
Cuts at: 6527 8
Size: 43
Fragments arranged by size:
1629 1425 889 472 421 346 282 275
243 189 159 54 50 43 34 33
18
EagI C'GGCC_G
Cuts at: 1537 1812 2019 2053 4009 1537
Size: 275 207 34 1956 4090
Fragments arranged by size:
4090 1956 275 207 34
EarI CTCTTCn'nnn_
Cuts at: 1353 3560 4492 1353
Size: 2207 932 3423
Fragments arranged by size:
3423 2207 932
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2192 2213 2453 5149 5523 5669
Size: 1064 138 603 21 240 2696 374 146
Cuts at: 5669 387
Size: 1280
Fragments arranged by size:
2696 1280 1064 603 374 240 146 138
21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2697 2774 2776 3426 3656 3673 4625
Size: 393 1065 77 2 650 230 17 952
Cuts at: 4625 5222 6009 6277 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1065 952 787 650 597 393 268
230 77 17 2
EcoO109I rG'GnC_Cy
Cuts at: 2502 2634 2840 2941 3274 2502
Size: 132 206 101 333 5790
Fragments arranged by size:
5790 333 206 132 101
EcoRI G'AATT_C
Cuts at: 3871 4249 3871
Size: 378 6184
FspI TGC'GCA
Cuts at: 5068 5068
Size: 6562
HaeII r_GCGC'y
Cuts at: 1678 2452 2473 3579 5709 1678
Size: 774 21 1106 2130 2531
Fragments arranged by size:
2531 2130 1106 774 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2240 2272 2288
Size: 32 135 32 599 32 37 32 16
Cuts at: 2288 2320 2422 2454 5203 5235 5277 5309 1373
Size: 32 102 32 2749 32 42 32 2626
Fragments arranged by size:
2749 2626 599 135 102 42 37 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2986 3906 4152 669
Size: 988 281 1048 920 246 3079
Fragments arranged by size:
3079 1048 988 920 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6562
HpaI GTT'AAC
Cuts at: 4152 4152
Size: 6562
KasI G'GCGC_C
Cuts at: 2469 2469
Size: 6562
KpnI G_GTAC'C
Cuts at: 3887 3887
Size: 6562
MfeI C'AATT_G
Cuts at: 4161 4161
Size: 6562
MluI A'CGCG_T
Cuts at: 6438 6438
Size: 6562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3147 3422 5676 5860 1305
Size: 1842 275 2254 184 2007
Fragments arranged by size:
2254 2007 1842 275 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2527 2873 2906 3486 3768
Size: 54 1425 343 695 346 33 580 282
Cuts at: 3768 10
Size: 2804
Fragments arranged by size:
2804 1425 695 580 346 343 282 54
33
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 2352 3239 4520 4879 5038 518
Size: 648 1161 25 887 1281 359 159 2042
Fragments arranged by size:
2042 1281 1161 887 648 359 159 25
MspA1I CmG'CkG
Cuts at: 1606 2058 2078 2644 3029 3978 4655 5803 6048
Size: 452 20 566 385 949 677 1148 245
Cuts at: 6048 1606
Size: 2120
Fragments arranged by size:
2120 1148 949 677 566 452 385 245
20
NaeI GCC'GGC
Cuts at: 2113 2468 2113
Size: 355 6207
NarI GG'CG_CC
Cuts at: 2470 2470
Size: 6562
NcoI C'CATG_G
Cuts at: 513 1065 2421 2490 2814 3769 513
Size: 552 1356 69 324 955 3306
Fragments arranged by size:
3306 1356 955 552 324 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6562
NgoMIV G'CCGG_C
Cuts at: 2111 2466 2111
Size: 355 6207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6562
NotI GC'GGCC_GC
Cuts at: 4009 4009
Size: 6562
NspI r_CATG'y
Cuts at: 1132 1229 2351 2668 2800 3920 5465 1132
Size: 97 1122 317 132 1120 1545 2229
Fragments arranged by size:
2229 1545 1122 1120 317 132 97
PasI CC'CwG_GG
Cuts at: 1348 2264 2537 3113 3823 1348
Size: 916 273 576 710 4087
Fragments arranged by size:
4087 916 710 576 273
PciI A'CATG_T
Cuts at: 5461 5461
Size: 6562
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2708 1108
Size: 1600 4962
PfoI T'CCnGG_A
Cuts at: 2306 2674 2306
Size: 368 6194
PmeI GTTT'AAAC
Cuts at: 3866 3866
Size: 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 6144 6176 4631
Size: 32 1481 32 5017
Fragments arranged by size:
5017 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2502 2840 2941 2502
Size: 338 101 6123
Fragments arranged by size:
6123 338 101
PshAI GACnn'nnGTC
Cuts at: 1613 2157 1613
Size: 544 6018
PsiI TTA'TAA
Cuts at: 4132 4132
Size: 6562
PspOMI G'GGCC_C
Cuts at: 2633 3274 2633
Size: 641 5921
PstI C_TGCA'G
Cuts at: 830 2187 2898 3914 830
Size: 1357 711 1016 3478
Fragments arranged by size:
3478 1357 1016 711
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6562
PvuII CAG'CTG
Cuts at: 2644 2644
Size: 6562
SalI G'TCGA_C
Cuts at: 1936 3904 1936
Size: 1968 4594
SanDI GG'GwC_CC
Cuts at: 2941 2941
Size: 6562
SbfI CC_TGCA'GG
Cuts at: 3914 3914
Size: 6562
ScaI AGT'ACT
Cuts at: 4810 6522 4810
Size: 1712 4850
SexAI A'CCwGG_T
Cuts at: 3300 3300
Size: 6562
SfcI C'TryA_G
Cuts at: 826 1045 2068 2183 2639 2894 3197 3517 3910
Size: 219 1023 115 456 255 303 320 393
Cuts at: 3910 5045 5726 5917 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1023 681 456 393 320 303
255 219 191 115
SfoI GGC'GCC
Cuts at: 2471 2471
Size: 6562
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
SgrAI Cr'CCGG_yG
Cuts at: 1700 2466 1700
Size: 766 5796
SmaI CCC'GGG
Cuts at: 1872 2921 3889 1872
Size: 1049 968 4545
Fragments arranged by size:
4545 1049 968
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4666 5567 5829 820
Size: 197 926 2723 901 262 1553
Fragments arranged by size:
2723 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2404 4353 493
Size: 1911 1949 2702
Fragments arranged by size:
2702 1949 1911
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6562
SphI G_CATG'C
Cuts at: 1132 2351 2800 3920 1132
Size: 1219 449 1120 3774
Fragments arranged by size:
3774 1219 1120 449
SspI AAT'ATT
Cuts at: 5 52 4486 5
Size: 47 4434 2081
Fragments arranged by size:
4434 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6562
StyI C'CwwG_G
Cuts at: 513 1065 2421 2490 2814 2994 3769 4001 513
Size: 552 1356 69 324 180 775 232 3074
Fragments arranged by size:
3074 1356 775 552 324 232 180 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3090 3228 3449 3504 4589 4606 4759
Size: 1255 1510 138 221 55 1085 17 153
Cuts at: 4759 4944 5389 325
Size: 185 445 1498
Fragments arranged by size:
1510 1498 1255 1085 445 221 185 153
138 55 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3360 4808 6520
Size: 274 80 33 51 1443 1383 1448 1712
Cuts at: 6520 96
Size: 138
Fragments arranged by size:
1712 1448 1443 1383 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3424 3456 6522 6554 3424
Size: 32 3066 32 3432
Fragments arranged by size:
3432 3066 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3713 1316
Size: 2397 4165
XbaI T'CTAG_A
Cuts at: 3898 3898
Size: 6562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3479 3479
Size: 6562
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6562
XmaI C'CCGG_G
Cuts at: 1870 2919 3887 1870
Size: 1049 968 4545
Fragments arranged by size:
4545 1049 968
XmnI GAAnn'nnTTC
Cuts at: 2198 3569 4691 2198
Size: 1371 1122 4069
Fragments arranged by size:
4069 1371 1122
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4370 276
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SapI SfiI SrfI SwaI