Restriction map for
pFN21AA0933
(Circular) (Six-base) MAPSORT of: pFN21AA0933.seq Check: 9744 from: 1 to: 11446
pFN21AA0933 11446 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4278 5019 6178 6662 4278
Size: 741 1159 484 9062
Fragments arranged by size:
9062 1159 741 484
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9256 278
Size: 53 83 186 936 7720 2468
Fragments arranged by size:
7720 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4299 7011 8789 1937
Size: 2362 2712 1778 4594
Fragments arranged by size:
4594 2712 2362 1778
Acc65I G'GTAC_C
Cuts at: 8767 8767
Size: 11446
AclI AA'CG_TT
Cuts at: 1563 9573 9946 1563
Size: 8010 373 3063
Fragments arranged by size:
8010 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2043 2132 3107 3776 4406 5022 6197 7482
Size: 411 89 975 669 630 616 1175 1285
Cuts at: 7482 7948 9509 10893 11161 1632
Size: 466 1561 1384 268 1917
Fragments arranged by size:
1917 1561 1384 1285 1175 975 669 630
616 466 411 268 89
AfeI AGC'GCT
Cuts at: 5324 6053 8328 5324
Size: 729 2275 8442
Fragments arranged by size:
8442 2275 729
AflII C'TTAA_G
Cuts at: 820 1017 3562 820
Size: 197 2545 8704
Fragments arranged by size:
8704 2545 197
AflIII A'CryG_T
Cuts at: 1203 2871 3596 5223 5763 10345 11322 1203
Size: 1668 725 1627 540 4582 977 1327
Fragments arranged by size:
4582 1668 1627 1327 977 725 540
AgeI A'CCGG_T
Cuts at: 10277 10277
Size: 11446
AhdI GACnn_n'nnGTC
Cuts at: 2974 3649 6618 10175 2974
Size: 675 2969 3557 4245
Fragments arranged by size:
4245 3557 2969 675
AleI CACnn'nnGTG
Cuts at: 1166 5033 1166
Size: 3867 7579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3558 4347 6104 1184
Size: 2374 789 1757 6526
Fragments arranged by size:
6526 2374 1757 789
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3655 3687 4833 4865 5213 5245 3655
Size: 32 1146 32 348 32 9856
Fragments arranged by size:
9856 1146 348 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2419 2945 4015 4090 4483 5263 6193 6920 7714
Size: 526 1070 75 393 780 930 727 794
Cuts at: 7714 9189 10761 2419
Size: 1475 1572 3104
Fragments arranged by size:
3104 1572 1475 1070 930 794 780 727
526 393 75
ApaI G_GGCC'C
Cuts at: 6088 6226 8408 6088
Size: 138 2182 9126
Fragments arranged by size:
9126 2182 138
ApaLI G'TGCA_C
Cuts at: 5286 7023 9503 10659 5286
Size: 1737 2480 1156 6073
Fragments arranged by size:
6073 2480 1737 1156
ApoI r'AATT_y
Cuts at: 1493 2083 2956 3151 3241 3301 5621 6864 8312
Size: 590 873 195 90 60 2320 1243 1448
Cuts at: 8312 8364 8466 8534 8755 8981 9133 11242 1493
Size: 52 102 68 221 226 152 2109 1697
Fragments arranged by size:
2320 2109 1697 1448 1243 873 590 226
221 195 152 102 90 68 60 52
AseI AT'TA_AT
Cuts at: 160 1032 10000 160
Size: 872 8968 1606
Fragments arranged by size:
8968 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11446
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 3667 3825 4817 4979 5075 6258
Size: 73 152 1572 158 992 162 96 1183
Cuts at: 6258 8771 1870
Size: 2513 4545
Fragments arranged by size:
4545 2513 1572 1183 992 162 158 152
96 73
AvrII C'CTAG_G
Cuts at: 6802 6802
Size: 11446
BamHI G'GATC_C
Cuts at: 2008 6818 8776 9143 2008
Size: 4810 1958 367 4311
Fragments arranged by size:
4810 4311 1958 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4769 5156 8767 10222 618
Size: 325 217 627 2982 387 3611 1455 1842
Fragments arranged by size:
3611 2982 1842 1455 627 387 325 217
BanII G_rGCy'C
Cuts at: 1391 2028 2480 3438 3783 3804 4056 4526 5076
Size: 637 452 958 345 21 252 470 550
Cuts at: 5076 5457 6088 6226 6277 7721 8408 8765 1391
Size: 381 631 138 51 1444 687 357 4072
Fragments arranged by size:
4072 1444 958 687 637 631 550 470
452 381 357 345 252 138 51 21
BbeI G_GCGC'C
Cuts at: 5160 5160
Size: 11446
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2599 2893 2973 3445 3599 4146 4506
Size: 971 700 294 80 472 154 547 360
Cuts at: 4506 5937 6335 7503 7954 928
Size: 1431 398 1168 451 4420
Fragments arranged by size:
4420 1431 1168 971 700 547 472 451
398 360 294 154 80
BbvCI CC'TCA_GC
Cuts at: 3546 7467 3546
Size: 3921 7525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3962 3996 9637 9671 1903
Size: 34 2025 34 5641 34 3678
Fragments arranged by size:
5641 3678 2025 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5647 7798 9338 10548 5647
Size: 2151 1540 1210 6545
Fragments arranged by size:
6545 2151 1540 1210
BclI T'GATC_A
Cuts at: 1548 5907 7978 1548
Size: 4359 2071 5016
Fragments arranged by size:
5016 4359 2071
BfrBI ATG'CAT
Cuts at: 2897 3080 6397 8712 2897
Size: 183 3317 2315 5631
Fragments arranged by size:
5631 3317 2315 183
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2182 5054 5336
Size: 107 122 71 631 605 510 2872 282
Cuts at: 5336 5516 10057 136
Size: 180 4541 1525
Fragments arranged by size:
4541 2872 1525 631 605 510 282 180
122 107 71
BglII A'GATC_T
Cuts at: 2484 2816 6141 8176 11441 2484
Size: 332 3325 2035 3265 2489
Fragments arranged by size:
3325 3265 2489 2035 332
BlpI GC'TnA_GC
Cuts at: 3611 5102 5264 7409 7707 7715 8863 3611
Size: 1491 162 2145 298 8 1148 6194
Fragments arranged by size:
6194 2145 1491 1148 298 162 8
Bme1580I G_kGCm'C
Cuts at: 1790 4980 5290 5771 6088 6226 7027 8408 9507
Size: 3190 310 481 317 138 801 1381 1099
Cuts at: 9507 10663 1790
Size: 1156 2573
Fragments arranged by size:
3190 2573 1381 1156 1099 801 481 317
310 138
BmgBI CAC'GTC
Cuts at: 1318 3061 3597 4825 5226 5881 1318
Size: 1743 536 1228 401 655 6883
Fragments arranged by size:
6883 1743 1228 655 536 401
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2226 2424 3496
Size: 416 53 83 399 24 809 198 1072
Cuts at: 3496 4471 7166 9177 10135 442
Size: 975 2695 2011 958 1753
Fragments arranged by size:
2695 2011 1753 1072 975 958 809 416
399 198 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11446
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3840 5014 5443 5882 3840
Size: 1174 429 439 9404
Fragments arranged by size:
9404 1174 439 429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4415 4566 5413 5441 5612 5729 5807 6166
Size: 3176 151 847 28 171 117 78 359
Cuts at: 6166 6561 7774 7790 10106 1239
Size: 395 1213 16 2316 2579
Fragments arranged by size:
3176 2579 2316 1213 847 395 359 171
151 117 78 28 16
Bpu10I CC'TnA_GC
Cuts at: 3546 5810 7467 3546
Size: 2264 1657 7525
Fragments arranged by size:
7525 2264 1657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3123 3835 4883 6709 7897 9571 10436 10734 3123
Size: 712 1048 1826 1188 1674 865 298 3835
Fragments arranged by size:
3835 1826 1674 1188 1048 865 712 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3433 4122 4310 5854 5935 6355 6781
Size: 617 1934 689 188 1544 81 420 426
Cuts at: 6781 8143 10109 882
Size: 1362 1966 2219
Fragments arranged by size:
2219 1966 1934 1544 1362 689 617 426
420 188 81
BsaAI yAC'GTr
Cuts at: 493 1204 5128 6313 9237 493
Size: 711 3924 1185 2924 2702
Fragments arranged by size:
3924 2924 2702 1185 711
BsaBI GATnn'nnATC
Cuts at: 4620 11440 4620
Size: 6820 4626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5157 6029 9253 9635
Size: 53 83 186 936 3624 872 3224 382
Cuts at: 9635 10270 275
Size: 635 1451
Fragments arranged by size:
3624 3224 1451 936 872 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3796 9879 10277 10551 10698 11109 3796
Size: 6083 398 274 147 411 4133
Fragments arranged by size:
6083 4133 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2639 2669 4555 4585 4700
Size: 30 165 30 1236 30 1886 30 115
Cuts at: 4700 4730 4833 4863 5213 5243 5669 5699 7282
Size: 30 103 30 350 30 426 30 1583
Cuts at: 7282 7312 7463 7493 1178
Size: 30 151 30 5131
Fragments arranged by size:
5131 1886 1583 1236 426 350 165 151
115 103 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2801 3989 5227 5453 5684 6125 6245
Size: 991 624 1188 1238 226 231 441 120
Cuts at: 6245 6788 7484 8094 8444 1186
Size: 543 696 610 350 4188
Fragments arranged by size:
4188 1238 1188 991 696 624 610 543
441 350 231 226 120
BseYI C'CCAG_C
Cuts at: 2662 4160 5366 6951 6996 7688 10649 11145 2662
Size: 1498 1206 1585 45 692 2961 496 2963
Fragments arranged by size:
2963 2961 1585 1498 1206 692 496 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3554 4744 5903 6335 7040 1163
Size: 573 1818 1190 1159 432 705 5569
Fragments arranged by size:
5569 1818 1190 1159 705 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2644 4917 8896 9657 10685 1540
Size: 275 186 643 2273 3979 761 1028 2301
Fragments arranged by size:
3979 2301 2273 1028 761 643 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 3438 3522 3612 3804 3813 4526 4851 5010
Size: 1410 84 90 192 9 713 325 159
Cuts at: 5010 5178 5290 5457 5604 6156 7027 7721 9507
Size: 168 112 167 147 552 871 694 1786
Cuts at: 9507 9592 10663 2028
Size: 85 1071 2811
Fragments arranged by size:
2811 1786 1410 1071 871 713 694 552
325 192 168 167 159 147 112 90
85 84 9
BsmI GAATG_Cn'
Cuts at: 2832 3371 6424 6700 8450 8714 8957 9050 2832
Size: 539 3053 276 1750 264 243 93 5228
Fragments arranged by size:
5228 3053 1750 539 276 264 243 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2480 3438 3522 3612 3783 3804
Size: 399 238 452 958 84 90 171 21
Cuts at: 3804 3813 4056 4526 4851 4980 5010 5076 5178
Size: 9 243 470 325 129 30 66 102
Cuts at: 5178 5290 5457 5604 5771 6088 6156 6226 6277
Size: 112 167 147 167 317 68 70 51
Cuts at: 6277 7027 7721 8408 8765 9507 9592 10663 1391
Size: 750 694 687 357 742 85 1071 2174
Fragments arranged by size:
2174 1071 958 750 742 694 687 470
452 399 357 325 317 243 238 171
167 167 147 129 112 102 90 85
84 70 68 66 51 30 21 9
BspEI T'CCGG_A
Cuts at: 11109 11109
Size: 11446
BspHI T'CATG_A
Cuts at: 4395 7852 8592 9335 11065 4395
Size: 3457 740 743 1730 4776
Fragments arranged by size:
4776 3457 1730 743 740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4278 5019 5262 5553 5875 6178 6662 7341
Size: 3434 741 243 291 322 303 484 679
Cuts at: 7341 8801 844
Size: 1460 3489
Fragments arranged by size:
3489 3434 1460 741 679 484 322 303
291 243
BsrBI CCG'CTC
Cuts at: 1394 1823 4995 9333 1394
Size: 429 3172 4338 3507
Fragments arranged by size:
4338 3507 3172 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4575 4599 7374 7509 9941 10115 65
Size: 1183 3327 24 2775 135 2432 174 1396
Fragments arranged by size:
3327 2775 2432 1396 1183 174 135 24
BsrFI r'CCGG_y
Cuts at: 1700 1810 2371 4358 7379 10090 10277 1700
Size: 110 561 1987 3021 2711 187 2869
Fragments arranged by size:
3021 2869 2711 1987 561 187 110
BsrGI T'GTAC_A
Cuts at: 96 2319 6036 7228 7530 96
Size: 2223 3717 1192 302 4012
Fragments arranged by size:
4012 3717 2223 1192 302
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11446
BssSI C'ACGA_G
Cuts at: 4110 6040 9506 10518 4110
Size: 1930 3466 1012 5038
Fragments arranged by size:
5038 3466 1930 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2419 5536 6160 6313 6430 8581 794
Size: 1625 3117 624 153 117 2151 3659
Fragments arranged by size:
3659 3117 2151 1625 624 153 117
BstBI TT'CG_AA
Cuts at: 10250 10250
Size: 11446
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11446
BstXI CCAn_nnnn'nTGG
Cuts at: 2299 4456 5299 2299
Size: 2157 843 8446
Fragments arranged by size:
8446 2157 843
BstYI r'GATC_y
Cuts at: 1972 2008 2110 2484 2816 3004 4683 6141 6818
Size: 36 102 374 332 188 1679 1458 677
Cuts at: 6818 8176 8688 8776 9143 9528 9545 10997 11083
Size: 1358 512 88 367 385 17 1452 86
Cuts at: 11083 11095 11441 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1679 1458 1452 1358 677 512 385
374 367 346 332 188 102 88 86
36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2159 3513 4986 5616 7440 2159
Size: 1354 1473 630 1824 6165
Fragments arranged by size:
6165 1824 1473 1354 630
BtgI C'CryG_G
Cuts at: 513 1065 2004 3423 5152 5298 6076 6663 6741
Size: 552 939 1419 1729 146 778 587 78
Cuts at: 6741 513
Size: 5218
Fragments arranged by size:
5218 1729 1419 939 778 587 552 146
78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10707
Fragments arranged by size:
10707 683 56
BtsI GCAGTG_nn'
Cuts at: 2408 3723 4891 4980 6936 8962 9756 9776 2408
Size: 1315 1168 89 1956 2026 794 20 4078
Fragments arranged by size:
4078 2026 1956 1315 1168 794 89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11446
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11411
DraI TTT'AAA
Cuts at: 2278 6838 8750 2278
Size: 4560 1912 4974
Fragments arranged by size:
4974 4560 1912
DraIII CAC_nnn'GTG
Cuts at: 7063 7063
Size: 11446
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5885 8669 10453 809
Size: 507 4569 2784 1784 1802
Fragments arranged by size:
4569 2784 1802 1784 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2574 2641
Size: 54 1425 50 275 18 471 273 67
Cuts at: 2641 4440 4914 5203 5722 5937 6063 8126 8893
Size: 1799 474 289 519 215 126 2063 767
Cuts at: 8893 9782 11411 8
Size: 889 1629 43
Fragments arranged by size:
2063 1799 1629 1425 889 767 519 474
471 289 275 273 215 126 67 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2641 4914 8893 1537
Size: 275 829 2273 3979 4090
Fragments arranged by size:
4090 3979 2273 829 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2062 2151 2466 3983 4308 4346 4998
Size: 657 52 89 315 1517 325 38 652
Cuts at: 4998 5106 5268 6711 6829 7073 7390 8522 9376
Size: 108 162 1443 118 244 317 1132 854
Cuts at: 9376 1353
Size: 3423
Fragments arranged by size:
3423 1517 1443 1132 854 657 652 325
317 315 244 162 118 108 89 52
38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2123 2579 5459 6251 7507 10033
Size: 1064 138 534 456 2880 792 1256 2526
Cuts at: 10033 10407 10553 387
Size: 374 146 1280
Fragments arranged by size:
2880 2526 1280 1256 1064 792 534 456
374 146 138
EcoICRI GAG'CTC
Cuts at: 2026 3436 3802 4524 5455 7719 2026
Size: 1410 366 722 931 2264 5753
Fragments arranged by size:
5753 2264 1410 931 722 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2132 3107 3776 4406 4415 4566
Size: 393 411 89 975 669 630 9 151
Cuts at: 4566 5022 5413 5441 5612 5729 5807 6166 6197
Size: 456 391 28 171 117 78 359 31
Cuts at: 6197 6561 7482 7774 7790 7948 9509 10106 10893
Size: 364 921 292 16 158 1561 597 787
Cuts at: 10893 11161 1239
Size: 268 1524
Fragments arranged by size:
1561 1524 975 921 787 669 630 597
456 411 393 391 364 359 292 268
171 158 151 117 89 78 31 28
16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2471 4307 4723 4901 6092 6107 6559 7064 7559
Size: 1836 416 178 1191 15 452 505 495
Cuts at: 7559 2471
Size: 6358
Fragments arranged by size:
6358 1836 1191 505 495 452 416 178
15
EcoO109I rG'GnC_Cy
Cuts at: 3134 3174 4728 5461 5569 5713 5755 6084 6085
Size: 40 1554 733 108 144 42 329 1
Cuts at: 6085 7474 7765 8404 8405 3134
Size: 1389 291 639 1 6175
Fragments arranged by size:
6175 1554 1389 733 639 329 291 144
108 42 40 1 1
EcoRI G'AATT_C
Cuts at: 5621 8466 8534 8755 9133 5621
Size: 2845 68 221 378 7934
Fragments arranged by size:
7934 2845 378 221 68
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2073 2073
Size: 11446
FspI TGC'GCA
Cuts at: 2831 4972 9952 2831
Size: 2141 4980 4325
Fragments arranged by size:
4980 4325 2141
FspAI rTGC'GCAy
Cuts at: 2831 2831
Size: 11446
HaeII r_GCGC'y
Cuts at: 1678 4490 5160 5326 6055 6303 7707 7948 8330
Size: 2812 670 166 729 248 1404 241 382
Cuts at: 8330 10593 1678
Size: 2263 2531
Fragments arranged by size:
2812 2531 2263 1404 729 670 382 248
241 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2172 2204 2808
Size: 32 135 32 530 32 38 32 604
Cuts at: 2808 2840 2960 2992 3022 3054 3301 3333 3635
Size: 32 120 32 30 32 247 32 302
Cuts at: 3635 3667 4133 4165 4555 4587 4654 4686 5183
Size: 32 466 32 390 32 67 32 497
Cuts at: 5183 5210 5215 5242 5621 5645 5653 5677 6604
Size: 27 5 27 379 24 8 24 927
Cuts at: 6604 6636 6880 6912 7097 7129 8011 8043 10087
Size: 32 244 32 185 32 882 32 2044
Cuts at: 10087 10119 10161 10193 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2044 927 882 604 530 497 466
390 379 302 247 244 185 135 120
67 42 38 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 30 27 27
24 24 8 5
HincII GTy'rAC
Cuts at: 669 1657 1938 6736 6912 7012 8790 9036 669
Size: 988 281 4798 176 100 1778 246 3079
Fragments arranged by size:
4798 3079 1778 988 281 246 176 100
HindIII A'AGCT_T
Cuts at: 748 5356 748
Size: 4608 6838
HpaI GTT'AAC
Cuts at: 6736 9036 6736
Size: 2300 9146
KasI G'GCGC_C
Cuts at: 5156 5156
Size: 11446
KpnI G_GTAC'C
Cuts at: 8771 8771
Size: 11446
MfeI C'AATT_G
Cuts at: 9045 9045
Size: 11446
MluI A'CGCG_T
Cuts at: 11322 11322
Size: 11446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 2691 5712 8017 8400 10560 10744 1305
Size: 1209 177 3021 2305 383 2160 184 2007
Fragments arranged by size:
3021 2305 2160 2007 1209 383 184 177
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 2576 4442 5724 5939
Size: 54 1425 343 471 273 1866 1282 215
Cuts at: 5939 6065 8128 10
Size: 126 2063 3328
Fragments arranged by size:
3328 2063 1866 1425 1282 471 343 273
215 126 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4067 5033 5297 7133 9404 9763 9922
Size: 648 2901 966 264 1836 2271 359 159
Cuts at: 9922 518
Size: 2042
Fragments arranged by size:
2901 2271 2042 1836 966 648 359 264
159
MspA1I CmG'CkG
Cuts at: 1606 2325 3660 3943 4348 5089 5494 5926 6160
Size: 719 1335 283 405 741 405 432 234
Cuts at: 6160 6917 7404 7711 8629 8862 9539 10687 10932
Size: 757 487 307 918 233 677 1148 245
Cuts at: 10932 1606
Size: 2120
Fragments arranged by size:
2120 1335 1148 918 757 741 719 677
487 432 405 405 307 283 245 234
233
NaeI GCC'GGC
Cuts at: 7381 7381
Size: 11446
NarI GG'CG_CC
Cuts at: 5157 5157
Size: 11446
NcoI C'CATG_G
Cuts at: 513 1065 2004 5298 6663 6741 513
Size: 552 939 3294 1365 78 5218
Fragments arranged by size:
5218 3294 1365 939 552 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11446
NgoMIV G'CCGG_C
Cuts at: 7379 7379
Size: 11446
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11446
NotI GC'GGCC_GC
Cuts at: 8893 8893
Size: 11446
NsiI A_TGCA'T
Cuts at: 2899 3082 6399 8714 2899
Size: 183 3317 2315 5631
Fragments arranged by size:
5631 3317 2315 183
NspI r_CATG'y
Cuts at: 1132 1229 2662 2875 3587 3874 4971 6397 7276
Size: 97 1433 213 712 287 1097 1426 879
Cuts at: 7276 7888 8804 10349 1132
Size: 612 916 1545 2229
Fragments arranged by size:
2229 1545 1433 1426 1097 916 879 712
612 287 213 97
PasI CC'CwG_GG
Cuts at: 1348 4789 6337 6451 1348
Size: 3441 1548 114 6343
Fragments arranged by size:
6343 3441 1548 114
PciI A'CATG_T
Cuts at: 2871 10345 2871
Size: 7474 3972
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4438 4483 1108
Size: 3330 45 8071
Fragments arranged by size:
8071 3330 45
PfoI T'CCnGG_A
Cuts at: 5639 5784 7578 7767 8241 5639
Size: 145 1794 189 474 8844
Fragments arranged by size:
8844 1794 474 189 145
PmeI GTTT'AAAC
Cuts at: 8750 8750
Size: 11446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2520 4833 4865 5213 5245 5507 5539 9515
Size: 32 2313 32 348 32 262 32 3976
Cuts at: 9515 9547 11028 11060 2488
Size: 32 1481 32 2874
Fragments arranged by size:
3976 2874 2313 1481 348 262 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4728 5569 5755 7474 7765 4728
Size: 841 186 1719 291 8409
Fragments arranged by size:
8409 1719 841 291 186
PshAI GACnn'nnGTC
Cuts at: 1613 5753 1613
Size: 4140 7306
PsiI TTA'TAA
Cuts at: 9016 9016
Size: 11446
PspOMI G'GGCC_C
Cuts at: 6084 6222 8404 6084
Size: 138 2182 9126
Fragments arranged by size:
9126 2182 138
PspXI vC'TCGA_Gb
Cuts at: 2095 3667 3825 2095
Size: 1572 158 9716
Fragments arranged by size:
9716 1572 158
PstI C_TGCA'G
Cuts at: 830 2415 2439 3194 3985 4557 4866 5191 5259
Size: 1585 24 755 791 572 309 325 68
Cuts at: 5259 5550 5988 6165 6189 6309 6375 6673 7041
Size: 291 438 177 24 120 66 298 368
Cuts at: 7041 7338 7495 8798 830
Size: 297 157 1303 3478
Fragments arranged by size:
3478 1585 1303 791 755 572 438 368
325 309 298 297 291 177 157 120
68 66 24 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11446
PvuII CAG'CTG
Cuts at: 2325 3943 4348 5926 7404 7711 8629 2325
Size: 1618 405 1578 1478 307 918 5142
Fragments arranged by size:
5142 1618 1578 1478 918 405 307
SacI G_AGCT'C
Cuts at: 2028 3438 3804 4526 5457 7721 2028
Size: 1410 366 722 931 2264 5753
Fragments arranged by size:
5753 2264 1410 931 722 366
SalI G'TCGA_C
Cuts at: 1936 7010 8788 1936
Size: 5074 1778 4594
Fragments arranged by size:
5074 4594 1778
SapI GCTCTTCn'nnn_
Cuts at: 2010 2151 4346 4998 5106 6711 7390 2010
Size: 141 2195 652 108 1605 679 6066
Fragments arranged by size:
6066 2195 1605 679 652 141 108
SbfI CC_TGCA'GG
Cuts at: 3985 5259 7338 8798 3985
Size: 1274 2079 1460 6633
Fragments arranged by size:
6633 2079 1460 1274
ScaI AGT'ACT
Cuts at: 2399 9694 11406 2399
Size: 7295 1712 2439
Fragments arranged by size:
7295 2439 1712
SexAI A'CCwGG_T
Cuts at: 7059 7059
Size: 11446
SfcI C'TryA_G
Cuts at: 826 1045 2411 2435 3190 3981 4553 4862 5187
Size: 219 1366 24 755 791 572 309 325
Cuts at: 5187 5255 5546 5984 6161 6185 6305 6371 6669
Size: 68 291 438 177 24 120 66 298
Cuts at: 6669 7037 7334 7491 8794 9929 10610 10801 826
Size: 368 297 157 1303 1135 681 191 1471
Fragments arranged by size:
1471 1366 1303 1135 791 755 681 572
438 368 325 309 298 297 291 219
191 177 157 120 68 66 24 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 5516 5516
Size: 11446
SfoI GGC'GCC
Cuts at: 5158 5158
Size: 11446
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11446
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11446
SmaI CCC'GGG
Cuts at: 1872 6260 8773 1872
Size: 4388 2513 4545
Fragments arranged by size:
4545 4388 2513
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2095 3138 3562 3667 3814 3825
Size: 197 926 152 1043 424 105 147 11
Cuts at: 3825 4898 6688 7912 9550 10451 10713 820
Size: 1073 1790 1224 1638 901 262 1553
Fragments arranged by size:
1790 1638 1553 1224 1073 1043 926 901
424 262 197 152 147 105 11
SnaBI TAC'GTA
Cuts at: 493 9237 493
Size: 8744 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11446
SphI G_CATG'C
Cuts at: 1132 3587 3874 4971 6397 7276 8804 1132
Size: 2455 287 1097 1426 879 1528 3774
Fragments arranged by size:
3774 2455 1528 1426 1097 879 287
SspI AAT'ATT
Cuts at: 5 52 9370 5
Size: 47 9318 2081
Fragments arranged by size:
9318 2081 47
StuI AGG'CCT
Cuts at: 1517 2441 3560 4250 5519 7445 1517
Size: 924 1119 690 1269 1926 5518
Fragments arranged by size:
5518 1926 1269 1119 924 690
StyI C'CwwG_G
Cuts at: 513 1065 2004 2304 3000 3202 4687 5298 6663
Size: 552 939 300 696 202 1485 611 1365
Cuts at: 6663 6741 6802 7389 8684 8885 513
Size: 78 61 587 1295 201 3074
Fragments arranged by size:
3074 1485 1365 1295 939 696 611 587
552 300 202 201 78 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2864 3245 4334 5305 6397 9473 9490
Size: 1255 1284 381 1089 971 1092 3076 17
Cuts at: 9490 9643 9828 10273 325
Size: 153 185 445 1498
Fragments arranged by size:
3076 1498 1284 1255 1092 1089 971 445
381 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 2397 2655
Size: 274 80 33 51 1443 342 78 258
Cuts at: 2655 3937 6036 7228 7530 9692 11404 96
Size: 1282 2099 1192 302 2162 1712 138
Fragments arranged by size:
2162 2099 1712 1443 1282 1192 342 302
274 258 138 80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4700 4732 8247 8279 11406 11438 4700
Size: 32 3515 32 3127 32 4708
Fragments arranged by size:
4708 3515 3127 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3949 6506 1316
Size: 2633 2557 6256
Fragments arranged by size:
6256 2633 2557
XbaI T'CTAG_A
Cuts at: 6598 7450 8470 8782 6598
Size: 852 1020 312 9262
Fragments arranged by size:
9262 1020 852 312
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5380 5647 5380
Size: 267 11179
XhoI C'TCGA_G
Cuts at: 1943 2095 3667 3825 1943
Size: 152 1572 158 9564
Fragments arranged by size:
9564 1572 158 152
XmaI C'CCGG_G
Cuts at: 1870 6258 8771 1870
Size: 4388 2513 4545
Fragments arranged by size:
4545 4388 2513
XmnI GAAnn'nnTTC
Cuts at: 2399 2751 7907 8148 9575 2399
Size: 352 5156 241 1427 4270
Fragments arranged by size:
5156 4270 1427 352 241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9254 276
Size: 53 83 186 936 7720 2468
Fragments arranged by size:
7720 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BsiWI BsmBI BstZ17I EcoRV FseI NruI
PacI PmlI PsrI RsrII SacII SanDI SrfI SwaI